RchiOBHm_Chr6g0296521

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0004566
Physical Location & Seq
Forward (+)
58288500 .. 58291704
3205 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26616

Sequence Viewer

Length: 1461 bp
ATGACGATGGGTGATAACAAGAACCAGATGCTTCAACACAACCAAGTATTTGATGTCTCAGTCAATATGCCGCCACAGGGAGGCTCCAAGTGCTTTGATGATGATGGCCGTCTCAAAAGAACTGGAACTGTTTGGACTGCCAGTGCTCACATAATTACTGCTGTAATTGGGTCTGGAGTTCTATCCTTGGCTTGGGCCATAGCTCAGCTTGGATGGGTGGCTGGCCCTGCTGTCATGTTCTTGTTCTCTTTGGTGACTTACTACACTTCAACTCTGCTCTCTGCTTGCTACCGCTCCAATGATCCTGACACTGGAAAAAGGAACTACACTTACATGGATGCTGTTCAATCTAACCTAGGTGGAGCTAAGGTCAAGATTTGTGGATATGTTCAGTACTTGAACCTTTTTGGAGTTGCCATTGGATACACTATAGCATCATCTATTAGCATGATGGCAATCAAGAGGTCTAACTGCTTCCACAAAAGTGGTGGAAAAGATCCATGCCATGTCAACAGCAACCCCTACATGATTGCATTTGGCATAGCAGAAATTATATTCTCTCAGATTCCAGACTTTGATCAGTTGTGGTGGCTTTCCATTGTTGCTGCAGTCATGTCCTTCACTTACTCCACAATTGGACTTGCCCTTGGAATTGCTAAAGTTGCAGAAACTGGAACAATCATGGGAAGTATGACCGGAATAAGCATTGGCACTGTGACTGAAACCCAAAAGATGTGGAGGAGCTTCCAAGCTCTTGGTGACATAGCTTTTGCCTACTCTTACTCTCTTATTTTGATTGAAATTCAGGACACAGTTAGATCTCCACCATCTGAAGCCAAGACAATGAAGAAGGCCACTATAATTAGTGTTGCAGTGACAACCCTTTTCTACATGCTATGTGGCTGCATGGGCTATGCTGCTTTTGGAGACTTATCCCCTGGAAACTTGCTCACTGGTTTTGGCTTCTATAACCCATATTGGCTCTTAGACATTGCCAACGCTGCTATAGTAGTCCACCTTGTTGGTGCATACCAAGTTTACTGCCAACCCTTATTTGCCTTTGTTGAGAAAGCAGCAGCGAAAAAGTATCCGGAGAGCGAATTTATATCAAGAGAAATCAAAATCCCAATTCCCGGTCTTGGCCATTGCAATCTTAACCTCTTCAGATTGGTGTGGAGGACTGCCTTTGTGATTGTGACCACTGTAATCTCTATGCTCCTCCCATTCTTTAATGATGTGGTTGGACTTCTTGGAGCTTTGGGATTCTGGCCATTGACTGTTTACTTCCCAGTTGAGATGTACATTGTGCAGAAGAGGATACCAAAGTGGAGCACAAGATGGCTTTGCCTCCAAATCCTAAGTGTTGCTTGCCTTATAATAACCATAGCTGCTGCTGCTGGCTCAATTGCTGGGGTGATTTCTGACCTCAAGATCTACAAGCCATTCAAGACCAGCTATTGA

Protein Analysis

486

Amino Acids

53.25

Weight (kDa)

8.8

Isoelectric Point (pI)

37.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 40 - 473 1.1e-125 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1376
AccBSI CCGCTC 1 cut(s) 294
AccIII TCCGGA 1 cut(s) 1090
AciI CCGC 2 cut(s) 71, 292
AclWI GGATC 2 cut(s) 296, 491
AcoI YGGCCR 3 cut(s) 106, 1141, 1268
AcsI RAATTY 2 cut(s) 801, 1100
AcuI CTGAAG 2 cut(s) 852, 1147
AfaI GTAC 2 cut(s) 395, 1301
AfiI CCNNNNNNNGG 6 cut(s) 77, 80, 192, 311, 1133, 1139
AgsI TTSAA 6 cut(s) 35, 270, 347, 400, 800, 1447
AjnI CCWGG 1 cut(s) 937
AleI CACNNNNGTG 1 cut(s) 483
AluBI AGCT 9 cut(s) 203, 208, 365, 744, 752, 767, 1256, 1388, 1455
AluI AGCT 9 cut(s) 203, 208, 365, 744, 752, 767, 1256, 1388, 1455
Alw21I GWGCWC 2 cut(s) 148, 1334
Alw26I GTCTC 3 cut(s) 61, 116, 921
AlwI GGATC 2 cut(s) 296, 491
AlwNI CAGNNNCTG 1 cut(s) 671
Aor13HI TCCGGA 1 cut(s) 1090
AoxI GGCC 6 cut(s) 106, 195, 223, 852, 1141, 1268
ApeKI GCWGC 9 cut(s) 605, 903, 917, 1001, 1073, 1076, 1388, 1391, 1394
ApoI RAATTY 2 cut(s) 801, 1100
ArsI GACNNNNNNTTYG 2 cut(s) 752, 784
AspA2I CCTAGG 1 cut(s) 355
AspS9I GGNCC 2 cut(s) 195, 224
AsuC2I CCSGG 1 cut(s) 1134
AsuHPI GGTGA 4 cut(s) 23, 265, 770, 1426
AvrII CCTAGG 1 cut(s) 355
BaeI ACNNNNGTAYC 2 cut(s) 1310, 1343
BalI TGGCCA 2 cut(s) 1143, 1270
Bbv12I GWGCWC 2 cut(s) 148, 1334
BbvI GCAGC 9 cut(s) 592, 890, 904, 988, 1085, 1088, 1375, 1378, 1381
BccI CCATC 5 cut(s) 98, 207, 445, 835, 1332
BceAI ACGGC 1 cut(s) 93
BciT130I CCWGG 1 cut(s) 939
BciVI GTATCC 3 cut(s) 416, 1098, 1311
BclI TGATCA 1 cut(s) 577
BcnI CCSGG 1 cut(s) 1134
BcoDI GTCTC 3 cut(s) 61, 116, 921
BfaI CTAG 1 cut(s) 356
BfmI CTRYAG 3 cut(s) 429, 606, 1005
BfuI GTATCC 3 cut(s) 416, 1098, 1311
BglII AGATCT 2 cut(s) 818, 1431
BlnI CCTAGG 1 cut(s) 355
BlpI GCTNAGC 1 cut(s) 204
BmcAI AGTACT 1 cut(s) 395
Bme1390I CCNGG 2 cut(s) 939, 1134
BmgT120I GGNCC 2 cut(s) 195, 224
BmiI GGNNCC 1 cut(s) 85
BmrFI CCNGG 2 cut(s) 939, 1134
BmrI ACTGGG 1 cut(s) 1283
BmsI GCATC 3 cut(s) 18, 328, 443
BmuI ACTGGG 1 cut(s) 1283
BpmI CTGGAG 1 cut(s) 195
Bpu10I CCTNAGC 1 cut(s) 366
Bpu1102I GCTNAGC 1 cut(s) 204
BpuEI CTTGAG 1 cut(s) 1412
BpuMI CCSGG 1 cut(s) 1134
BsaBI GATNNNNATC 1 cut(s) 455
BsaJI CCNNGG 4 cut(s) 186, 355, 646, 937
BsaWI WCCGGW 2 cut(s) 695, 1090
BsaXI ACNNNNNCTCC 2 cut(s) 354, 384
Bsc4I CCNNNNNNNGG 6 cut(s) 77, 80, 192, 311, 1133, 1139
Bse1I ACTGG 6 cut(s) 127, 141, 316, 676, 958, 1289
Bse3DI GCAATG 2 cut(s) 990, 1144
Bse8I GATNNNNATC 1 cut(s) 455
BseAI TCCGGA 1 cut(s) 1090
BseBI CCWGG 1 cut(s) 939
BseDI CCNNGG 4 cut(s) 186, 355, 646, 937
BseGI GGATG 2 cut(s) 218, 343
BseJI GATNNNNATC 1 cut(s) 455
BseLI CCNNNNNNNGG 6 cut(s) 77, 80, 192, 311, 1133, 1139
BseMI GCAATG 2 cut(s) 990, 1144
BseMII CTCAG 3 cut(s) 72, 218, 575
BseNI ACTGG 6 cut(s) 127, 141, 316, 676, 958, 1289
BseRI GAGGAG 2 cut(s) 754, 1208
BseXI GCAGC 9 cut(s) 592, 890, 904, 988, 1085, 1088, 1375, 1378, 1381
BseYI CCCAGC 1 cut(s) 1409
BsgI GTGCAG 1 cut(s) 1328
BshFI GGCC 6 cut(s) 108, 197, 225, 854, 1143, 1270
BsiHKAI GWGCWC 2 cut(s) 148, 1334
BsiSI CCGG 3 cut(s) 696, 1091, 1134
BslI CCNNNNNNNGG 6 cut(s) 77, 80, 192, 311, 1133, 1139
BsmAI GTCTC 3 cut(s) 61, 116, 921
BsmBI CGTCTC 1 cut(s) 116
BsnI GGCC 6 cut(s) 108, 197, 225, 854, 1143, 1270
Bsp1286I GDGCHC 2 cut(s) 148, 1334
Bsp13I TCCGGA 1 cut(s) 1090
Bsp1407I TGTACA 1 cut(s) 1299
Bsp143I GATC 5 cut(s) 301, 496, 577, 818, 1431
Bsp1720I GCTNAGC 1 cut(s) 204
BspACI CCGC 2 cut(s) 71, 292
BspANI GGCC 6 cut(s) 108, 197, 225, 854, 1143, 1270
BspCNI CTCAG 3 cut(s) 71, 217, 574
BspEI TCCGGA 1 cut(s) 1090
BspLI GGNNCC 1 cut(s) 85
BspMAI CTGCAG 1 cut(s) 610
BspPI GGATC 2 cut(s) 296, 491
BsrBI CCGCTC 1 cut(s) 294
BsrDI GCAATG 2 cut(s) 990, 1144
BsrGI TGTACA 1 cut(s) 1299
BsrI ACTGG 6 cut(s) 127, 141, 316, 676, 958, 1289
BssECI CCNNGG 4 cut(s) 186, 355, 646, 937
BssMI GATC 5 cut(s) 301, 496, 577, 818, 1431
BssT1I CCWWGG 3 cut(s) 186, 355, 646
Bst2UI CCWGG 1 cut(s) 939
Bst4CI ACNGT 5 cut(s) 130, 715, 814, 1204, 1279
Bst6I CTCTTC 2 cut(s) 1166, 1307
BstAUI TGTACA 1 cut(s) 1299
BstC8I GCNNGC 4 cut(s) 223, 286, 1369, 1399
BstDEI CTNAG 6 cut(s) 58, 204, 366, 561, 985, 1358
BstF5I GGATG 2 cut(s) 218, 343
BstKTI GATC 5 cut(s) 304, 499, 580, 821, 1434
BstMAI GTCTC 3 cut(s) 61, 116, 921
BstMBI GATC 5 cut(s) 301, 496, 577, 818, 1431
BstMWI GCNNNNNNNGC 6 cut(s) 90, 227, 662, 909, 1001, 1394
BstNI CCWGG 1 cut(s) 939
BstNSI RCATGY 1 cut(s) 895
BstSCI CCNGG 2 cut(s) 937, 1132
BstSFI CTRYAG 3 cut(s) 429, 606, 1005
BstV1I GCAGC 9 cut(s) 592, 890, 904, 988, 1085, 1088, 1375, 1378, 1381
BstX2I RGATCY 3 cut(s) 496, 818, 1431
BstXI CCANNNNNNTGG 3 cut(s) 485, 755, 1022
BstYI RGATCY 3 cut(s) 496, 818, 1431
BsuI GTATCC 3 cut(s) 416, 1098, 1311
BsuRI GGCC 6 cut(s) 108, 197, 225, 854, 1143, 1270
BtsCI GGATG 2 cut(s) 218, 343
BtsI GCAGTG 1 cut(s) 879
BtsIMutI CAGTG 6 cut(s) 148, 309, 711, 879, 951, 1200
Cac8I GCNNGC 4 cut(s) 223, 286, 1369, 1399
CaiI CAGNNNCTG 1 cut(s) 671
Cfr13I GGNCC 2 cut(s) 195, 224
Csp6I GTAC 2 cut(s) 394, 1300
CspCI CAANNNNNGTGG 6 cut(s) 361, 396, 469, 504, 716, 751
CviQI GTAC 2 cut(s) 394, 1300
DdeI CTNAG 6 cut(s) 58, 204, 366, 561, 985, 1358
DpnI GATC 5 cut(s) 303, 498, 579, 820, 1433
DpnII GATC 5 cut(s) 301, 496, 577, 818, 1431
EaeI YGGCCR 3 cut(s) 106, 1141, 1268
Eam1104I CTCTTC 2 cut(s) 1166, 1307
EarI CTCTTC 2 cut(s) 1166, 1307
Eco130I CCWWGG 3 cut(s) 186, 355, 646
Eco57I CTGAAG 2 cut(s) 852, 1147
EcoRII CCWGG 1 cut(s) 937
EcoT14I CCWWGG 3 cut(s) 186, 355, 646
ErhI CCWWGG 3 cut(s) 186, 355, 646
Esp3I CGTCTC 1 cut(s) 116
FalI AAGNNNNNCTT 2 cut(s) 1351, 1383
FbaI TGATCA 1 cut(s) 577
FokI GGATG 2 cut(s) 225, 350
FspBI CTAG 1 cut(s) 356
GsaI CCCAGC 1 cut(s) 1413
GsuI CTGGAG 1 cut(s) 195
HaeIII GGCC 6 cut(s) 108, 197, 225, 854, 1143, 1270
HapII CCGG 3 cut(s) 696, 1091, 1134
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HinfI GANTC 2 cut(s) 565, 1263
HpaII CCGG 3 cut(s) 696, 1091, 1134
HphI GGTGA 4 cut(s) 23, 265, 770, 1426
Hpy166II GTNNAC 4 cut(s) 511, 1015, 1039, 1282
Hpy188I TCNGA 4 cut(s) 564, 832, 1166, 1423
Hpy8I GTNNAC 4 cut(s) 511, 1015, 1039, 1282
HpyAV CCTTC 2 cut(s) 628, 844
HpyCH4III ACNGT 5 cut(s) 130, 715, 814, 1204, 1279
HpyCH4V TGCA 8 cut(s) 533, 608, 665, 872, 906, 1028, 1149, 1309
HpyF10VI GCNNNNNNNGC 6 cut(s) 90, 227, 662, 909, 1001, 1394
HpyF3I CTNAG 6 cut(s) 58, 204, 366, 561, 985, 1358
Kpn2I TCCGGA 1 cut(s) 1090
Ksp22I TGATCA 1 cut(s) 577
Kzo9I GATC 5 cut(s) 301, 496, 577, 818, 1431
LmnI GCTCC 7 cut(s) 89, 299, 362, 741, 1221, 1253, 1329
Lsp1109I GCAGC 9 cut(s) 592, 890, 904, 988, 1085, 1088, 1375, 1378, 1381
LweI GCATC 3 cut(s) 18, 328, 443
MaeI CTAG 1 cut(s) 356
MaeIII GTNAC 5 cut(s) 253, 715, 758, 874, 1195
MalI GATC 5 cut(s) 303, 498, 579, 820, 1433
MbiI CCGCTC 1 cut(s) 294
MboI GATC 5 cut(s) 301, 496, 577, 818, 1431
MboII GAAGA 3 cut(s) 859, 1153, 1324
MfeI CAATTG 2 cut(s) 633, 1404
MflI RGATCY 3 cut(s) 496, 818, 1431
MhlI GDGCHC 2 cut(s) 148, 1334
MlsI TGGCCA 2 cut(s) 1143, 1270
MluNI TGGCCA 2 cut(s) 1143, 1270
MmeI TCCRAC 1 cut(s) 1222
MnlI CCTC 9 cut(s) 74, 456, 732, 1169, 1170, 1229, 1308, 1358, 1436
Mox20I TGGCCA 2 cut(s) 1143, 1270
MroI TCCGGA 1 cut(s) 1090
MscI TGGCCA 2 cut(s) 1143, 1270
MseI TTAA 2 cut(s) 1155, 1230
MslI CAYNNNNRTG 2 cut(s) 332, 483
Msp20I TGGCCA 2 cut(s) 1143, 1270
MspI CCGG 3 cut(s) 696, 1091, 1134
MspR9I CCNGG 2 cut(s) 939, 1134
MunI CAATTG 2 cut(s) 633, 1404
MvaI CCWGG 1 cut(s) 939
MwoI GCNNNNNNNGC 6 cut(s) 90, 227, 662, 909, 1001, 1394
NciI CCSGG 1 cut(s) 1134
NdeII GATC 5 cut(s) 301, 496, 577, 818, 1431
NlaIV GGNNCC 1 cut(s) 85
NmuCI GTSAC 5 cut(s) 253, 715, 758, 874, 1195
NspI RCATGY 1 cut(s) 895
OliI CACNNNNGTG 1 cut(s) 483
PfeI GAWTC 2 cut(s) 565, 1263
PsiI TTATAA 1 cut(s) 1376
Psp6I CCWGG 1 cut(s) 937
PspFI CCCAGC 1 cut(s) 1409
PspGI CCWGG 1 cut(s) 937
PspN4I GGNNCC 1 cut(s) 85
PspPI GGNCC 2 cut(s) 195, 224
PsrI GAACNNNNNNTAC 2 cut(s) 314, 346
PstI CTGCAG 1 cut(s) 610
PstNI CAGNNNCTG 1 cut(s) 671
PsuI RGATCY 3 cut(s) 496, 818, 1431
RsaI GTAC 2 cut(s) 395, 1301
RsaNI GTAC 2 cut(s) 394, 1300
RseI CAYNNNNRTG 2 cut(s) 332, 483
SaqAI TTAA 2 cut(s) 1155, 1230
Sau3AI GATC 5 cut(s) 301, 496, 577, 818, 1431
Sau96I GGNCC 2 cut(s) 195, 224
ScaI AGTACT 1 cut(s) 395
ScrFI CCNGG 2 cut(s) 939, 1134
SduI GDGCHC 2 cut(s) 148, 1334
SfaNI GCATC 3 cut(s) 18, 328, 443
SfcI CTRYAG 3 cut(s) 429, 606, 1005
SmiMI CAYNNNNRTG 2 cut(s) 332, 483
SmlI CTYRAG 1 cut(s) 1427
SmoI CTYRAG 1 cut(s) 1427
SsiI CCGC 2 cut(s) 71, 292
SspMI CTAG 1 cut(s) 356
StyD4I CCNGG 2 cut(s) 937, 1132
StyI CCWWGG 3 cut(s) 186, 355, 646
TaaI ACNGT 5 cut(s) 130, 715, 814, 1204, 1279
TatI WGTACW 2 cut(s) 393, 1299
TauI GCSGC 1 cut(s) 73
TfiI GAWTC 2 cut(s) 565, 1263
Tru1I TTAA 2 cut(s) 1155, 1230
Tru9I TTAA 2 cut(s) 1155, 1230
TscAI CASTG 6 cut(s) 148, 316, 718, 879, 958, 1207
TseFI GTSAC 5 cut(s) 253, 715, 758, 874, 1195
TseI GCWGC 9 cut(s) 605, 903, 917, 1001, 1073, 1076, 1388, 1391, 1394
Tsp45I GTSAC 5 cut(s) 253, 715, 758, 874, 1195
TspDTI ATGAA 1 cut(s) 860
TspRI CASTG 6 cut(s) 148, 316, 718, 879, 958, 1207
XapI RAATTY 2 cut(s) 801, 1100
XceI RCATGY 1 cut(s) 895
XcmI CCANNNNNNNNNTGG 1 cut(s) 485
XmaJI CCTAGG 1 cut(s) 355
XspI CTAG 1 cut(s) 356
ZrmI AGTACT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.