AT2G03620

magnesium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004962
Physical Location & Seq
Reverse (-)
1100325 .. 1103495
3171 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G03620.1

Sequence Viewer

Length: 1266 bp
ATGGGAGAACAACTAGATCCATTTTCCGCTTCTAATCTTCCGGATTTCATATCTTCTCAAAAAATTGGTAGACCGGTTAATTTCGAAGGACAAACTAACCGTGGGCATCCTTTTTCTGGTCTTAAGAAGAGAGGTCAATCTAGTCGTTCTTGGGTGAAAATTGATCAAGATGGGAACTCTGCGGTTTTGGAGCTCGACAAGGCGACTATAATGAAGCGTTGTTCCTTACCATCCAGGGATTTGAGACTCCTTGACCCTTTGTTTATCTATCCCTCGAGTATTTTGGGGCGTGAGAGAGCGATAGTTGTCAGTCTTGAGAAGATCAGGTGTATTATCACAGCTGAAGAGGTCATTCTCATGAATGCTCGAGATGCATCTGTTGTTCAGTACCAGTCGGAACTGTGTAAACGCCTTCAATCAAACCACAACCTAAATGTTAAAGATGATTTGCCATTTGAATTTAAAGCGTTGGAGCTGGTTTTGGAACTATCTTGCTTGTCTTTAGATGCACAGGTGAATGAGCTTGAGATGGAGGTGTATCCCGTTCTTGATGAACTAGCCACAAACATCAGCACCCTTAATCTAGAACACGTGCGCAGGCTAAAAGGTCGTCTTCTTACCTTAACACAGAAAGTTCAGAAGGTTTGTGATGAAATAGAGCATTTAATGGATGATGATGATGACATGGCTGAGATGTATTTGACTGAGAAAAAAGAAAGAGCTGAAGCTCATGCGTCAGAGGAGCTAGAAGATAACATTGGTGAGGACTTTGAATCTTCTGGAATAGTTTCGAAGTCTGCGCCGGTTTCACCTGTGGGCTCAACAAGTGGAAACTTTGGGAAGCTTCAAAGAGCCTTTAGCAGCATTGTTGGCTCACATAAAAGCTTGTTAAGCTCCTCTAGCATCGGAGAGAACATTGATCAACTAGAGATGTTACTAGAAGCTTACTTTGTCGTTGTTGATAACACTCTGAGCAAATTATCATCGCTGAAAGAGTATATAGATGATACAGAGGATCTGATCAACATTAAGCTGGGGAATGTGCAGAATCAGTTGATTCAGTTTCAACTGCTTCTCACCGCGGCAACCTTTGTGGCAGCTATTTTTGCCGCTGTAACCGCTGTCTTCGGGATGAATTTGCAAGACTCTGTTTTCCAGAATCCGACGACTTTCCAGTATGTTTTACTGATAACAGGTATTGGATGCGGTTTTTTGTATTTTGGTTTTGTGTTATACTTCAAGCACAAGAAAGTATTCCCTCTCTGA

Protein Analysis

421

Amino Acids

47.03

Weight (kDa)

4.95

Isoelectric Point (pI)

40.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRS2-like PF22099 55 - 247 7.1e-32 Magnesium transporter MRS2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 596
AccI GTMKAC 1 cut(s) 70
AccII CGCG 1 cut(s) 1082
AccIII TCCGGA 1 cut(s) 40
AciI CCGC 7 cut(s) 27, 182, 1080, 1082, 1110, 1119, 1206
AclWI GGATC 2 cut(s) 11, 1023
AcsI RAATTY 2 cut(s) 458, 1135
AcuI CTGAAG 2 cut(s) 363, 744
AcvI CACGTG 1 cut(s) 592
AfaI GTAC 1 cut(s) 389
AfiI CCNNNNNNNGG 1 cut(s) 116
AflII CTTAAG 1 cut(s) 122
AflIII ACRYGT 1 cut(s) 589
AgeI ACCGGT 1 cut(s) 73
AgsI TTSAA 6 cut(s) 416, 458, 773, 848, 1067, 1240
AjnI CCWGG 1 cut(s) 233
AjuI GAANNNNNNNTTGG 2 cut(s) 741, 773
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 1 cut(s) 238
AlwI GGATC 2 cut(s) 11, 1023
Ama87I CYCGRG 2 cut(s) 274, 366
Aor13HI TCCGGA 1 cut(s) 40
ApeKI GCWGC 2 cut(s) 861, 1097
ApoI RAATTY 2 cut(s) 458, 1135
AsiGI ACCGGT 1 cut(s) 73
Asp700I GAANNNNTTC 2 cut(s) 787, 1253
AspLEI GCGC 2 cut(s) 597, 802
AsuHPI GGTGA 5 cut(s) 166, 526, 773, 801, 1069
AsuII TTCGAA 2 cut(s) 84, 791
AvaI CYCGRG 2 cut(s) 274, 366
BanII GRGCYC 2 cut(s) 195, 821
BbrPI CACGTG 1 cut(s) 592
BbsI GAAGAC 2 cut(s) 605, 1117
Bbv12I GWGCWC 1 cut(s) 195
BbvI GCAGC 2 cut(s) 873, 1109
BccI CCATC 3 cut(s) 164, 238, 523
BciT130I CCWGG 1 cut(s) 235
BciVI GTATCC 1 cut(s) 549
BclI TGATCA 3 cut(s) 163, 919, 1020
BcoDI GTCTC 1 cut(s) 238
BfaI CTAG 8 cut(s) 14, 141, 557, 584, 746, 900, 926, 938
BfrI CTTAAG 1 cut(s) 122
BfuI GTATCC 1 cut(s) 549
BisI GCNGC 4 cut(s) 862, 1083, 1098, 1110
BlsI GCNGC 4 cut(s) 863, 1084, 1099, 1111
Bme1390I CCNGG 1 cut(s) 235
BmeT110I CYCGRG 2 cut(s) 274, 366
BmrFI CCNGG 1 cut(s) 235
BmsI GCATC 6 cut(s) 115, 361, 383, 496, 912, 1193
BpiI GAAGAC 2 cut(s) 605, 1117
Bpu14I TTCGAA 2 cut(s) 84, 791
BpuEI CTTGAG 2 cut(s) 335, 545
BsaAI YACGTR 1 cut(s) 592
BsaJI CCNNGG 3 cut(s) 100, 234, 1080
BsaWI WCCGGW 2 cut(s) 40, 73
Bsc4I CCNNNNNNNGG 1 cut(s) 116
Bse118I RCCGGY 2 cut(s) 73, 802
Bse1I ACTGG 2 cut(s) 391, 1174
BseAI TCCGGA 1 cut(s) 40
BseBI CCWGG 1 cut(s) 235
BseDI CCNNGG 3 cut(s) 100, 234, 1080
BseGI GGATG 5 cut(s) 106, 230, 676, 1137, 1208
BseLI CCNNNNNNNGG 1 cut(s) 116
BseMII CTCAG 3 cut(s) 681, 696, 962
BseNI ACTGG 2 cut(s) 391, 1174
BseRI GAGGAG 2 cut(s) 755, 886
BseXI GCAGC 2 cut(s) 873, 1109
BseYI CCCAGC 1 cut(s) 1033
BsgI GTGCAG 1 cut(s) 1064
Bsh1236I CGCG 1 cut(s) 1082
BshTI ACCGGT 1 cut(s) 73
BsiHKAI GWGCWC 1 cut(s) 195
BsiHKCI CYCGRG 2 cut(s) 274, 366
BsiSI CCGG 3 cut(s) 41, 74, 803
BslI CCNNNNNNNGG 1 cut(s) 116
BsmAI GTCTC 1 cut(s) 238
BsmI GAATGC 1 cut(s) 367
BsoBI CYCGRG 2 cut(s) 274, 366
Bsp119I TTCGAA 2 cut(s) 84, 791
Bsp1286I GDGCHC 2 cut(s) 195, 821
Bsp13I TCCGGA 1 cut(s) 40
Bsp143I GATC 6 cut(s) 16, 163, 321, 919, 1015, 1020
BspACI CCGC 7 cut(s) 27, 182, 1080, 1082, 1110, 1119, 1206
BspCNI CTCAG 3 cut(s) 682, 697, 963
BspEI TCCGGA 1 cut(s) 40
BspFNI CGCG 1 cut(s) 1082
BspHI TCATGA 1 cut(s) 357
BspPI GGATC 2 cut(s) 11, 1023
BspT104I TTCGAA 2 cut(s) 84, 791
BspTI CTTAAG 1 cut(s) 122
BsrFI RCCGGY 2 cut(s) 73, 802
BsrI ACTGG 2 cut(s) 391, 1174
BssAI RCCGGY 2 cut(s) 73, 802
BssECI CCNNGG 3 cut(s) 100, 234, 1080
BssMI GATC 6 cut(s) 16, 163, 321, 919, 1015, 1020
Bst2UI CCWGG 1 cut(s) 235
Bst4CI ACNGT 2 cut(s) 101, 402
Bst6I CTCTTC 2 cut(s) 122, 339
BstAFI CTTAAG 1 cut(s) 122
BstBAI YACGTR 1 cut(s) 592
BstBI TTCGAA 2 cut(s) 84, 791
BstC8I GCNNGC 1 cut(s) 599
BstDEI CTNAG 3 cut(s) 690, 705, 971
BstDSI CCRYGG 2 cut(s) 100, 1080
BstF5I GGATG 5 cut(s) 106, 230, 676, 1137, 1208
BstFNI CGCG 1 cut(s) 1082
BstHHI GCGC 2 cut(s) 597, 802
BstKTI GATC 6 cut(s) 19, 166, 324, 922, 1018, 1023
BstMAI GTCTC 1 cut(s) 238
BstMBI GATC 6 cut(s) 16, 163, 321, 919, 1015, 1020
BstMWI GCNNNNNNNGC 6 cut(s) 371, 870, 891, 900, 1106, 1118
BstNI CCWGG 1 cut(s) 235
BstSCI CCNGG 1 cut(s) 233
BstUI CGCG 1 cut(s) 1082
BstV1I GCAGC 2 cut(s) 873, 1109
BstV2I GAAGAC 2 cut(s) 605, 1117
BstX2I RGATCY 2 cut(s) 16, 1015
BstYI RGATCY 2 cut(s) 16, 1015
BsuI GTATCC 1 cut(s) 549
BtgI CCRYGG 2 cut(s) 100, 1080
BtgZI GCGATG 1 cut(s) 969
BtsCI GGATG 5 cut(s) 106, 230, 676, 1137, 1208
Cac8I GCNNGC 1 cut(s) 599
CciI TCATGA 1 cut(s) 357
CfoI GCGC 2 cut(s) 597, 802
Cfr10I RCCGGY 2 cut(s) 73, 802
Cfr42I CCGCGG 1 cut(s) 1083
CseI GACGC 1 cut(s) 723
Csp6I GTAC 1 cut(s) 388
CspAI ACCGGT 1 cut(s) 73
CspCI CAANNNNNGTGG 2 cut(s) 1074, 1109
CviAII CATG 3 cut(s) 358, 685, 731
CviQI GTAC 1 cut(s) 388
DdeI CTNAG 3 cut(s) 690, 705, 971
DpnI GATC 6 cut(s) 18, 165, 323, 921, 1017, 1022
DpnII GATC 6 cut(s) 16, 163, 321, 919, 1015, 1020
DraI TTTAAA 1 cut(s) 463
Eam1104I CTCTTC 2 cut(s) 122, 339
EarI CTCTTC 2 cut(s) 122, 339
Ecl136II GAGCTC 1 cut(s) 193
Eco24I GRGCYC 2 cut(s) 195, 821
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 2 cut(s) 363, 744
Eco72I CACGTG 1 cut(s) 592
Eco88I CYCGRG 2 cut(s) 274, 366
EcoICRI GAGCTC 1 cut(s) 193
EcoRII CCWGG 1 cut(s) 233
EcoT22I ATGCAT 1 cut(s) 376
EcoT38I GRGCYC 2 cut(s) 195, 821
FaeI CATG 3 cut(s) 361, 688, 734
FalI AAGNNNNNCTT 2 cut(s) 597, 629
FatI CATG 3 cut(s) 357, 684, 730
FbaI TGATCA 3 cut(s) 163, 919, 1020
FblI GTMKAC 1 cut(s) 70
Fnu4HI GCNGC 4 cut(s) 862, 1083, 1098, 1110
FokI GGATG 5 cut(s) 93, 217, 683, 1144, 1215
FriOI GRGCYC 2 cut(s) 195, 821
Fsp4HI GCNGC 4 cut(s) 862, 1083, 1098, 1110
FspBI CTAG 8 cut(s) 14, 141, 557, 584, 746, 900, 926, 938
FspI TGCGCA 1 cut(s) 596
GlaI GCGC 2 cut(s) 596, 801
GluI GCNGC 4 cut(s) 862, 1083, 1098, 1110
GsaI CCCAGC 1 cut(s) 1037
HapII CCGG 3 cut(s) 41, 74, 803
HgaI GACGC 1 cut(s) 723
HhaI GCGC 2 cut(s) 597, 802
Hin1II CATG 3 cut(s) 361, 688, 734
Hin6I GCGC 2 cut(s) 595, 800
HinP1I GCGC 2 cut(s) 595, 800
HindIII AAGCTT 3 cut(s) 842, 883, 942
HinfI GANTC 6 cut(s) 246, 773, 1048, 1057, 1145, 1159
HpaII CCGG 3 cut(s) 41, 74, 803
HphI GGTGA 5 cut(s) 166, 526, 773, 801, 1069
Hpy166II GTNNAC 2 cut(s) 71, 407
Hpy188I TCNGA 8 cut(s) 397, 639, 739, 908, 972, 1020, 1164, 1265
Hpy8I GTNNAC 2 cut(s) 71, 407
Hpy99I CGWCG 1 cut(s) 1168
HpyAV CCTTC 3 cut(s) 80, 422, 634
HpyCH4III ACNGT 2 cut(s) 101, 402
HpyCH4IV ACGT 1 cut(s) 591
HpyCH4V TGCA 4 cut(s) 374, 509, 1045, 1141
HpyF10VI GCNNNNNNNGC 6 cut(s) 371, 870, 891, 900, 1106, 1118
HpyF3I CTNAG 3 cut(s) 690, 705, 971
HpySE526I ACGT 1 cut(s) 591
Hsp92II CATG 3 cut(s) 361, 688, 734
HspAI GCGC 2 cut(s) 595, 800
Kpn2I TCCGGA 1 cut(s) 40
Ksp22I TGATCA 3 cut(s) 163, 919, 1020
KspI CCGCGG 1 cut(s) 1083
Kzo9I GATC 6 cut(s) 16, 163, 321, 919, 1015, 1020
LmnI GCTCC 4 cut(s) 190, 472, 742, 899
Lsp1109I GCAGC 2 cut(s) 873, 1109
LweI GCATC 6 cut(s) 115, 361, 383, 496, 912, 1193
MaeI CTAG 8 cut(s) 14, 141, 557, 584, 746, 900, 926, 938
MaeII ACGT 1 cut(s) 591
MaeIII GTNAC 2 cut(s) 933, 1114
MalI GATC 6 cut(s) 18, 165, 323, 921, 1017, 1022
MboI GATC 6 cut(s) 16, 163, 321, 919, 1015, 1020
MboII GAAGA 9 cut(s) 29, 45, 139, 331, 356, 605, 761, 768, 1117
MflI RGATCY 2 cut(s) 16, 1015
MhlI GDGCHC 2 cut(s) 195, 821
MluCI AATT 6 cut(s) 63, 79, 159, 458, 977, 1135
MlyI GAGTC 2 cut(s) 240, 1139
MmeI TCCRAC 3 cut(s) 375, 450, 1187
MnlI CCTC 8 cut(s) 125, 283, 340, 526, 733, 757, 907, 1006
Mph1103I ATGCAT 1 cut(s) 376
MroI TCCGGA 1 cut(s) 40
MroXI GAANNNNTTC 2 cut(s) 787, 1253
MseI TTAA 9 cut(s) 78, 123, 438, 462, 579, 623, 665, 890, 1029
MslI CAYNNNNRTG 1 cut(s) 356
MspA1I CMGCKG 4 cut(s) 341, 1082, 1112, 1121
MspCI CTTAAG 1 cut(s) 122
MspI CCGG 3 cut(s) 41, 74, 803
MspR9I CCNGG 1 cut(s) 235
Mva1269I GAATGC 1 cut(s) 367
MvaI CCWGG 1 cut(s) 235
MvnI CGCG 1 cut(s) 1082
MwoI GCNNNNNNNGC 6 cut(s) 371, 870, 891, 900, 1106, 1118
NdeII GATC 6 cut(s) 16, 163, 321, 919, 1015, 1020
NlaIII CATG 3 cut(s) 361, 688, 734
NsbI TGCGCA 1 cut(s) 596
NsiI ATGCAT 1 cut(s) 376
NspV TTCGAA 2 cut(s) 84, 791
PaeR7I CTCGAG 2 cut(s) 274, 366
PagI TCATGA 1 cut(s) 357
PctI GAATGC 1 cut(s) 367
PdmI GAANNNNTTC 2 cut(s) 787, 1253
PfeI GAWTC 4 cut(s) 773, 1048, 1057, 1159
PinAI ACCGGT 1 cut(s) 73
PkrI GCNGC 4 cut(s) 863, 1084, 1099, 1111
PleI GAGTC 2 cut(s) 240, 1139
PmaCI CACGTG 1 cut(s) 592
PmlI CACGTG 1 cut(s) 592
PpsI GAGTC 2 cut(s) 240, 1139
Ppu21I YACGTR 1 cut(s) 592
Psp124BI GAGCTC 1 cut(s) 195
Psp6I CCWGG 1 cut(s) 233
PspCI CACGTG 1 cut(s) 592
PspFI CCCAGC 1 cut(s) 1033
PspGI CCWGG 1 cut(s) 233
PspXI VCTCGAGB 1 cut(s) 274
PsuI RGATCY 2 cut(s) 16, 1015
PvuII CAGCTG 1 cut(s) 341
RsaI GTAC 1 cut(s) 389
RsaNI GTAC 1 cut(s) 388
RseI CAYNNNNRTG 1 cut(s) 356
SacI GAGCTC 1 cut(s) 195
SacII CCGCGG 1 cut(s) 1083
SaqAI TTAA 9 cut(s) 78, 123, 438, 462, 579, 623, 665, 890, 1029
SatI GCNGC 4 cut(s) 862, 1083, 1098, 1110
Sau3AI GATC 6 cut(s) 16, 163, 321, 919, 1015, 1020
SchI GAGTC 2 cut(s) 240, 1139
ScrFI CCNGG 1 cut(s) 235
SduI GDGCHC 2 cut(s) 195, 821
SfaNI GCATC 6 cut(s) 115, 361, 383, 496, 912, 1193
Sfr274I CTCGAG 2 cut(s) 274, 366
Sfr303I CCGCGG 1 cut(s) 1083
SfuI TTCGAA 2 cut(s) 84, 791
SgrBI CCGCGG 1 cut(s) 1083
SlaI CTCGAG 2 cut(s) 274, 366
SmiMI CAYNNNNRTG 1 cut(s) 356
SmlI CTYRAG 5 cut(s) 122, 274, 314, 366, 524
SmoI CTYRAG 5 cut(s) 122, 274, 314, 366, 524
Sse9I AATT 6 cut(s) 63, 79, 159, 458, 977, 1135
SsiI CCGC 7 cut(s) 27, 182, 1080, 1082, 1110, 1119, 1206
SspMI CTAG 8 cut(s) 14, 141, 557, 584, 746, 900, 926, 938
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 1 cut(s) 233
TaaI ACNGT 2 cut(s) 101, 402
TaiI ACGT 1 cut(s) 594
TaqI TCGA 5 cut(s) 84, 195, 275, 367, 791
TasI AATT 6 cut(s) 63, 79, 159, 458, 977, 1135
TauI GCSGC 2 cut(s) 1085, 1112
TfiI GAWTC 4 cut(s) 773, 1048, 1057, 1159
Tru1I TTAA 9 cut(s) 78, 123, 438, 462, 579, 623, 665, 890, 1029
Tru9I TTAA 9 cut(s) 78, 123, 438, 462, 579, 623, 665, 890, 1029
TseI GCWGC 2 cut(s) 861, 1097
TspDTI ATGAA 6 cut(s) 37, 227, 374, 567, 666, 1148
Vha464I CTTAAG 1 cut(s) 122
XapI RAATTY 2 cut(s) 458, 1135
XbaI TCTAGA 1 cut(s) 583
XhoI CTCGAG 2 cut(s) 274, 366
XmiI GTMKAC 1 cut(s) 70
XmnI GAANNNNTTC 2 cut(s) 787, 1253
XspI CTAG 8 cut(s) 14, 141, 557, 584, 746, 900, 926, 938
Zsp2I ATGCAT 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.