MD13G1062600.v1.1

magnesium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004962
Physical Location & Seq
Forward (+)
4360378 .. 4363627
3250 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1062600.v1.1.491

Sequence Viewer

Length: 1266 bp
ATGGAAAGATCGCAATCTCGAACCCCTTTACTTCCTTCTGATCTTCTAGAATCCACCTCCTCTAACAACACTGAAAGCGCTCAGTTAAATGCACATGGAGTTCGTGGGCTGTCTGGTATTCAGGGACTGAAGAAGAGAGGTCACGGAAGTCGGTCTTGGATAAAGATTGATCCGCTTGGGAATATAAGCATTTTGGAGCTTGACAAGGCTACTATAATGAGACATTGTTCGTTGCCTGGAAGAGATCTCCGACTTTTGGATCCTTTGTTCATATACCCTTCTACAATATTAGGAAGGGAGAGAGCTATTGTTGTTAGTCTTGAACAGATCAGGTGTATAATCACCGCTGATGAGGTTATCCTGATGAATTCCCTGGATGGATGTGTTGTTCAGTACAAGTCTGAATTGTGCAAACGCCTAGAGTCAAACAAAGATCAATCTGATGACTTGCCTTTCGAGTTTAGAGCATTGGAGCTGGCTCTGGAACTAACCTGCATGTCTCTAGATGCTCAGGTAAGGGAACTGCAAATGGAGATATACCCTGTGCTGGATGAACTAGCATCATCAATAAACACTGTTAATCTGGAGCGTGTGCGTAGACTCAAAGGTCACCTTCTTGCATTGACTCAACGAGTTCAAAAGGTCCGTGACGAAATAGAACATCTCATGGATGATGACGGTGACATGGCTGAGATGTACCTTACTGAGAAGAAACAAAGGTCAGAGGGTTATCCCTTAAGTGACCTTTGTTTTCAAACTAACACATCAGGCGAGGAAACAATTGTTTCAAAATCTGCTCCTGTTTCTCCAGTGAGGTCACTCAGTGGAGCACATAAATTGCAAAGGGCTTTTACAAGTTTTGTGAGTTCAAGCAGGAATGGAAGCTTATTGAGTTCATCTAATTTTGGAGAAAACATTGATAAACTGGAAATGCTGCTTGAAGCGTACTTTGTTGCCATCGACCATACTCTCAGCAAGTTGTCATCGCTGAAAGAATACATAGATGATACCGAAGACTTGATTAACTTTAAACTGGGCAATGTTCAGAACCATCTGATACAGTTTGAGTTGCTCCTTACAGCGGCTACCTTCTTGGCTACTGTTTTTGCCGCTGTTACAGCAGTATTTGGAATGAATTTACCCTCCACTGTTTTCGATAATCCATCTACATTCAATTGGGTTTTGGTTTTCAGTGGTGTTGCCTGTGGGTTCCTGTACTTCTCTTTTCTGATTTATTTTAGGTATAAAAGGATCTTTCCCTTGTAA

Protein Analysis

422

Amino Acids

47.28

Weight (kDa)

5.42

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRS2-like PF22099 65 - 244 4.6e-32 Magnesium transporter MRS2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 606
Acc36I ACCTGC 1 cut(s) 500
AccI GTMKAC 1 cut(s) 598
AciI CCGC 4 cut(s) 173, 345, 1082, 1110
AclWI GGATC 4 cut(s) 164, 254, 267, 1259
AcsI RAATTY 2 cut(s) 367, 1135
AcuI CTGAAG 1 cut(s) 149
AdeI CACNNNGTG 1 cut(s) 824
AfaI GTAC 4 cut(s) 395, 698, 947, 1217
AfeI AGCGCT 1 cut(s) 79
AfiI CCNNNNNNNGG 3 cut(s) 256, 547, 1081
AflII CTTAAG 1 cut(s) 736
AgsI TTSAA 7 cut(s) 323, 638, 755, 789, 870, 941, 1174
AjnI CCWGG 2 cut(s) 235, 372
AjuI GAANNNNNNNTTGG 2 cut(s) 139, 171
AloI GAACNNNNNNTCC 4 cut(s) 251, 283, 372, 404
AluBI AGCT 4 cut(s) 199, 305, 475, 885
AluI AGCT 4 cut(s) 199, 305, 475, 885
Alw21I GWGCWC 1 cut(s) 832
Alw26I GTCTC 2 cut(s) 214, 504
AlwI GGATC 4 cut(s) 164, 254, 267, 1259
AlwNI CAGNNNCTG 1 cut(s) 127
Aor51HI AGCGCT 1 cut(s) 79
ApeKI GCWGC 1 cut(s) 934
ApoI RAATTY 2 cut(s) 367, 1135
ArsI GACNNNNNNTTYG 2 cut(s) 437, 469
AspLEI GCGC 1 cut(s) 80
AspS9I GGNCC 1 cut(s) 643
AsuHPI GGTGA 3 cut(s) 334, 602, 692
AvaII GGWCC 1 cut(s) 643
BamHI GGATCC 1 cut(s) 259
BbsI GAAGAC 1 cut(s) 1020
Bbv12I GWGCWC 1 cut(s) 832
BbvI GCAGC 1 cut(s) 921
BccI CCATC 4 cut(s) 371, 965, 1059, 1171
BciT130I CCWGG 2 cut(s) 237, 374
BcoDI GTCTC 2 cut(s) 214, 504
BfaI CTAG 4 cut(s) 47, 419, 503, 557
BfoI RGCGCY 1 cut(s) 81
BfrI CTTAAG 1 cut(s) 736
BfuAI ACCTGC 1 cut(s) 500
BglII AGATCT 1 cut(s) 244
BisI GCNGC 3 cut(s) 935, 1083, 1110
BlsI GCNGC 3 cut(s) 936, 1084, 1111
Bme1390I CCNGG 2 cut(s) 237, 374
Bme18I GGWCC 1 cut(s) 643
BmgT120I GGNCC 1 cut(s) 643
BmiI GGNNCC 2 cut(s) 261, 1211
BmrFI CCNGG 2 cut(s) 237, 374
BmrI ACTGGG 1 cut(s) 1043
BmsI GCATC 2 cut(s) 496, 569
BmuI ACTGGG 1 cut(s) 1043
BpiI GAAGAC 1 cut(s) 1020
BpmI CTGGAG 2 cut(s) 605, 792
Bpu10I CCTNAGC 1 cut(s) 510
BsaBI GATNNNNATC 1 cut(s) 13
BsaJI CCNNGG 1 cut(s) 372
Bsc4I CCNNNNNNNGG 3 cut(s) 256, 547, 1081
Bse1I ACTGG 3 cut(s) 809, 930, 1038
Bse3DI GCAATG 1 cut(s) 1045
Bse8I GATNNNNATC 1 cut(s) 13
BseBI CCWGG 2 cut(s) 237, 374
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 4 cut(s) 382, 386, 556, 676
BseJI GATNNNNATC 1 cut(s) 13
BseLI CCNNNNNNNGG 3 cut(s) 256, 547, 1081
BseMI GCAATG 1 cut(s) 1045
BseMII CTCAG 6 cut(s) 95, 524, 681, 696, 835, 985
BseNI ACTGG 3 cut(s) 809, 930, 1038
BseRI GAGGAG 1 cut(s) 49
BseXI GCAGC 1 cut(s) 921
BsiHKAI GWGCWC 1 cut(s) 832
BslFI GGGAC 1 cut(s) 138
BslI CCNNNNNNNGG 3 cut(s) 256, 547, 1081
BsmAI GTCTC 2 cut(s) 214, 504
BsmFI GGGAC 1 cut(s) 138
Bsp1286I GDGCHC 1 cut(s) 832
Bsp143I GATC 8 cut(s) 8, 40, 169, 244, 259, 327, 433, 1251
BspACI CCGC 4 cut(s) 173, 345, 1082, 1110
BspCNI CTCAG 6 cut(s) 94, 523, 682, 697, 834, 984
BspLI GGNNCC 2 cut(s) 261, 1211
BspMI ACCTGC 1 cut(s) 500
BspPI GGATC 4 cut(s) 164, 254, 267, 1259
BspTI CTTAAG 1 cut(s) 736
BsrDI GCAATG 1 cut(s) 1045
BsrI ACTGG 3 cut(s) 809, 930, 1038
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 8 cut(s) 8, 40, 169, 244, 259, 327, 433, 1251
Bst2UI CCWGG 2 cut(s) 237, 374
Bst4CI ACNGT 5 cut(s) 577, 680, 1062, 1102, 1150
Bst6I CTCTTC 2 cut(s) 128, 235
BstAFI CTTAAG 1 cut(s) 736
BstC8I GCNNGC 1 cut(s) 477
BstDEI CTNAG 6 cut(s) 81, 510, 690, 705, 821, 971
BstEII GGTNACC 1 cut(s) 608
BstF5I GGATG 4 cut(s) 382, 386, 556, 676
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 8 cut(s) 11, 43, 172, 247, 262, 330, 436, 1254
BstMAI GTCTC 2 cut(s) 214, 504
BstMBI GATC 8 cut(s) 8, 40, 169, 244, 259, 327, 433, 1251
BstMWI GCNNNNNNNGC 1 cut(s) 1118
BstNI CCWGG 2 cut(s) 237, 374
BstNSI RCATGY 1 cut(s) 499
BstPI GGTNACC 1 cut(s) 608
BstSCI CCNGG 2 cut(s) 235, 372
BstV1I GCAGC 1 cut(s) 921
BstV2I GAAGAC 1 cut(s) 1020
BstX2I RGATCY 3 cut(s) 244, 259, 1251
BstYI RGATCY 3 cut(s) 244, 259, 1251
BtgZI GCGATG 1 cut(s) 969
BtsCI GGATG 4 cut(s) 382, 386, 556, 676
BtsIMutI CAGTG 6 cut(s) 69, 573, 816, 829, 1146, 1198
BveI ACCTGC 1 cut(s) 500
Cac8I GCNNGC 1 cut(s) 477
CaiI CAGNNNCTG 1 cut(s) 127
CfoI GCGC 1 cut(s) 80
Cfr13I GGNCC 1 cut(s) 643
Csp6I GTAC 4 cut(s) 394, 697, 946, 1216
CviAII CATG 4 cut(s) 95, 496, 667, 685
CviQI GTAC 4 cut(s) 394, 697, 946, 1216
DdeI CTNAG 6 cut(s) 81, 510, 690, 705, 821, 971
DpnI GATC 8 cut(s) 10, 42, 171, 246, 261, 329, 435, 1253
DpnII GATC 8 cut(s) 8, 40, 169, 244, 259, 327, 433, 1251
DraI TTTAAA 1 cut(s) 1030
DraIII CACNNNGTG 1 cut(s) 824
DrdI GACNNNNNNGTC 1 cut(s) 606
DseDI GACNNNNNNGTC 1 cut(s) 606
Eam1104I CTCTTC 2 cut(s) 128, 235
EarI CTCTTC 2 cut(s) 128, 235
Eco47I GGWCC 1 cut(s) 643
Eco47III AGCGCT 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 149
Eco91I GGTNACC 1 cut(s) 608
EcoO65I GGTNACC 1 cut(s) 608
EcoRI GAATTC 1 cut(s) 367
EcoRII CCWGG 2 cut(s) 235, 372
FaeI CATG 4 cut(s) 98, 499, 670, 688
FalI AAGNNNNNCTT 4 cut(s) 139, 171, 597, 629
FaqI GGGAC 1 cut(s) 138
FatI CATG 4 cut(s) 94, 495, 666, 684
FblI GTMKAC 1 cut(s) 598
Fnu4HI GCNGC 3 cut(s) 935, 1083, 1110
FokI GGATG 4 cut(s) 389, 393, 563, 683
Fsp4HI GCNGC 3 cut(s) 935, 1083, 1110
FspBI CTAG 4 cut(s) 47, 419, 503, 557
GlaI GCGC 1 cut(s) 79
GluI GCNGC 3 cut(s) 935, 1083, 1110
GsuI CTGGAG 2 cut(s) 605, 792
HaeII RGCGCY 1 cut(s) 81
HhaI GCGC 1 cut(s) 80
Hin1II CATG 4 cut(s) 98, 499, 670, 688
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HindIII AAGCTT 1 cut(s) 883
HinfI GANTC 4 cut(s) 50, 422, 600, 625
HphI GGTGA 3 cut(s) 334, 602, 692
Hpy166II GTNNAC 1 cut(s) 599
Hpy188I TCNGA 8 cut(s) 40, 251, 403, 442, 724, 1047, 1056, 1230
Hpy188III TCNNGA 7 cut(s) 18, 47, 320, 361, 482, 503, 584
Hpy8I GTNNAC 1 cut(s) 599
HpyAV CCTTC 5 cut(s) 45, 288, 288, 623, 1099
HpyCH4III ACNGT 5 cut(s) 577, 680, 1062, 1102, 1150
HpyCH4V TGCA 6 cut(s) 92, 411, 495, 526, 620, 841
HpyF10VI GCNNNNNNNGC 1 cut(s) 1118
HpyF3I CTNAG 6 cut(s) 81, 510, 690, 705, 821, 971
Hsp92II CATG 4 cut(s) 98, 499, 670, 688
HspAI GCGC 1 cut(s) 78
Kzo9I GATC 8 cut(s) 8, 40, 169, 244, 259, 327, 433, 1251
LmnI GCTCC 6 cut(s) 196, 472, 586, 802, 827, 1077
Lsp1109I GCAGC 1 cut(s) 921
LweI GCATC 2 cut(s) 496, 569
MaeI CTAG 4 cut(s) 47, 419, 503, 557
MaeIII GTNAC 7 cut(s) 140, 608, 647, 680, 740, 816, 1114
MalI GATC 8 cut(s) 10, 42, 171, 246, 261, 329, 435, 1253
MboI GATC 8 cut(s) 8, 40, 169, 244, 259, 327, 433, 1251
MboII GAAGA 6 cut(s) 35, 142, 145, 252, 721, 1025
MfeI CAATTG 2 cut(s) 780, 1174
MflI RGATCY 3 cut(s) 244, 259, 1251
MhlI GDGCHC 1 cut(s) 832
MluCI AATT 7 cut(s) 367, 404, 780, 836, 901, 1135, 1174
MlyI GAGTC 3 cut(s) 431, 594, 619
MmeI TCCRAC 1 cut(s) 274
MnlI CCTC 8 cut(s) 67, 70, 131, 346, 718, 766, 807, 1153
MseI TTAA 5 cut(s) 86, 579, 737, 1023, 1029
MspA1I CMGCKG 3 cut(s) 347, 1082, 1112
MspCI CTTAAG 1 cut(s) 736
MspR9I CCNGG 2 cut(s) 237, 374
MunI CAATTG 2 cut(s) 780, 1174
MvaI CCWGG 2 cut(s) 237, 374
MwoI GCNNNNNNNGC 1 cut(s) 1118
NdeII GATC 8 cut(s) 8, 40, 169, 244, 259, 327, 433, 1251
NlaIII CATG 4 cut(s) 98, 499, 670, 688
NlaIV GGNNCC 2 cut(s) 261, 1211
NmuCI GTSAC 6 cut(s) 140, 608, 647, 680, 740, 816
NspI RCATGY 1 cut(s) 499
PfeI GAWTC 1 cut(s) 50
PkrI GCNGC 3 cut(s) 936, 1084, 1111
PleI GAGTC 3 cut(s) 430, 594, 619
PpsI GAGTC 3 cut(s) 430, 594, 619
Psp6I CCWGG 2 cut(s) 235, 372
PspEI GGTNACC 1 cut(s) 608
PspGI CCWGG 2 cut(s) 235, 372
PspN4I GGNNCC 2 cut(s) 261, 1211
PspPI GGNCC 1 cut(s) 643
PstNI CAGNNNCTG 1 cut(s) 127
PsuI RGATCY 3 cut(s) 244, 259, 1251
RsaI GTAC 4 cut(s) 395, 698, 947, 1217
RsaNI GTAC 4 cut(s) 394, 697, 946, 1216
SaqAI TTAA 5 cut(s) 86, 579, 737, 1023, 1029
SatI GCNGC 3 cut(s) 935, 1083, 1110
Sau3AI GATC 8 cut(s) 8, 40, 169, 244, 259, 327, 433, 1251
Sau96I GGNCC 1 cut(s) 643
SchI GAGTC 3 cut(s) 431, 594, 619
ScrFI CCNGG 2 cut(s) 237, 374
SduI GDGCHC 1 cut(s) 832
SfaNI GCATC 2 cut(s) 496, 569
SinI GGWCC 1 cut(s) 643
SmlI CTYRAG 1 cut(s) 736
SmoI CTYRAG 1 cut(s) 736
Sse9I AATT 7 cut(s) 367, 404, 780, 836, 901, 1135, 1174
SsiI CCGC 4 cut(s) 173, 345, 1082, 1110
SspI AATATT 1 cut(s) 288
SspMI CTAG 4 cut(s) 47, 419, 503, 557
StyD4I CCNGG 2 cut(s) 235, 372
TaaI ACNGT 5 cut(s) 577, 680, 1062, 1102, 1150
TaqI TCGA 4 cut(s) 19, 456, 960, 1155
TaqII GACCGA 1 cut(s) 141
TasI AATT 7 cut(s) 367, 404, 780, 836, 901, 1135, 1174
TatI WGTACW 2 cut(s) 393, 1215
TauI GCSGC 2 cut(s) 1085, 1112
TfiI GAWTC 1 cut(s) 50
Tru1I TTAA 5 cut(s) 86, 579, 737, 1023, 1029
Tru9I TTAA 5 cut(s) 86, 579, 737, 1023, 1029
TscAI CASTG 6 cut(s) 76, 580, 816, 829, 1153, 1198
TseFI GTSAC 6 cut(s) 140, 608, 647, 680, 740, 816
TseI GCWGC 1 cut(s) 934
Tsp45I GTSAC 6 cut(s) 140, 608, 647, 680, 740, 816
TspDTI ATGAA 5 cut(s) 259, 380, 567, 885, 1148
TspGWI ACGGA 2 cut(s) 159, 635
TspRI CASTG 6 cut(s) 76, 580, 816, 829, 1153, 1198
Vha464I CTTAAG 1 cut(s) 736
VpaK11BI GGWCC 1 cut(s) 643
XapI RAATTY 2 cut(s) 367, 1135
XbaI TCTAGA 2 cut(s) 46, 502
XceI RCATGY 1 cut(s) 499
XmiI GTMKAC 1 cut(s) 598
XspI CTAG 4 cut(s) 47, 419, 503, 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.