pycom13g05500

magnesium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0004962
Physical Location & Seq
Forward (+)
3656006 .. 3658000
1995 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g05500.1

Sequence Viewer

Length: 1050 bp
ATGAGACATTGTTCGTTGCCTGGAAGAGATCTCCGACTTTTGGATCCTTTGTTCATATACCCTTCTACAATATTAGGAAGGGAGAGAGCTATTGTTGTTAGTCTTGAACAGATCAGGTGTATAATCACCGCTGACGAGGTTATCCTGATGAATTCCCTGGATGGATGTGTTGTTCAGTACAAGTCTGAATTGTGCAAACGCCTAGAGTCAAACAAAGATCAATCTGATGACTTGCCTTTCGAGTTTAGAGCATTGGAGCTGGCTCTGGAACTAACCTGCATGTCTCTAGATGCTCAGGTAAAGGAACTGCAAATGGACATATACCCTGTGCTGGATGAACTAGCATCATCAATAAACACTGTTAATCTGGAGCGTGTGCGTAGACTCAAAGGTCACCTTCTTGCATTGACTCAACGAGTTCAAAAGGTCCGTGACGAAATAGAACATCTCATGGATGATGACGGTGACATGGCTGAGATGTACCTTACTGAGAAGAAACAAAGGTCAGAGGGTTATCCCTTAAGTGACCTTTGTTTTCAAACTAACACATCAGGCGAGGAAACAATTGTTTCAAAATCTGCTCCTGTTTCTCCAGTGGGGTCACTCAGTGGAGCACATCAATTGCAAAGGGCTTTTACTAGTTTTGTGAGTTCAAGCAGGAATGGAAGCTTGTTGAGTTCATCTAATTTCGGAGAAAACATTGATAAACTGGAAATGCTGCTTGAAGCCTACTTTGTTGGCATCGACCATACTCTCAACAAGTTGTTATCGCTGAAAGAATACATAGATGATACCGAAGACTTGATTAACTTTAAACTGGGCAATGTTCAGAACCATCTGATACAGTTTGAGTTGCTCCTTACAGCGGCTACCTTCTTGGCTACTGTTTTTGCCGCTGTTACAGCAGTATTTGGAATGAATTTACCCTCCACTGTTTTCGATAGTCCATCTACATTCAATTGGGTTTTGGTTTTCAGTGGTGTTGCCTGTGGGTTCCTGTACTTCTCTTTTCTGATTTATTTTAGGTATAAAAGGATCTTTCCCTTGTAA

Protein Analysis

350

Amino Acids

39.39

Weight (kDa)

4.93

Isoelectric Point (pI)

39.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRS2-like PF22099 2 - 172 2.2e-30 Magnesium transporter MRS2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 390
Acc36I ACCTGC 1 cut(s) 284
AccI GTMKAC 1 cut(s) 382
AciI CCGC 3 cut(s) 129, 866, 894
AclWI GGATC 3 cut(s) 38, 51, 1043
AcsI RAATTY 2 cut(s) 151, 919
AdeI CACNNNGTG 1 cut(s) 608
AfaI GTAC 3 cut(s) 179, 482, 1001
AfiI CCNNNNNNNGG 3 cut(s) 40, 331, 865
AflII CTTAAG 1 cut(s) 520
AgsI TTSAA 7 cut(s) 107, 422, 539, 573, 654, 725, 958
AhlI ACTAGT 1 cut(s) 638
AjnI CCWGG 2 cut(s) 19, 156
AloI GAACNNNNNNTCC 4 cut(s) 35, 67, 156, 188
AluBI AGCT 3 cut(s) 89, 259, 669
AluI AGCT 3 cut(s) 89, 259, 669
Alw21I GWGCWC 1 cut(s) 616
Alw26I GTCTC 1 cut(s) 288
AlwI GGATC 3 cut(s) 38, 51, 1043
ApeKI GCWGC 1 cut(s) 718
ApoI RAATTY 2 cut(s) 151, 919
ArsI GACNNNNNNTTYG 2 cut(s) 221, 253
AspS9I GGNCC 1 cut(s) 427
AsuHPI GGTGA 3 cut(s) 118, 386, 476
AvaII GGWCC 1 cut(s) 427
BamHI GGATCC 1 cut(s) 43
BbsI GAAGAC 1 cut(s) 804
Bbv12I GWGCWC 1 cut(s) 616
BbvI GCAGC 1 cut(s) 705
BccI CCATC 3 cut(s) 155, 843, 955
BciT130I CCWGG 2 cut(s) 21, 158
BcoDI GTCTC 1 cut(s) 288
BcuI ACTAGT 1 cut(s) 638
BfaI CTAG 4 cut(s) 203, 287, 341, 639
BfrI CTTAAG 1 cut(s) 520
BfuAI ACCTGC 1 cut(s) 284
BglII AGATCT 1 cut(s) 28
BisI GCNGC 3 cut(s) 719, 867, 894
BlsI GCNGC 3 cut(s) 720, 868, 895
Bme1390I CCNGG 2 cut(s) 21, 158
Bme18I GGWCC 1 cut(s) 427
BmgT120I GGNCC 1 cut(s) 427
BmiI GGNNCC 2 cut(s) 45, 995
BmrFI CCNGG 2 cut(s) 21, 158
BmrI ACTGGG 1 cut(s) 827
BmsI GCATC 3 cut(s) 280, 353, 750
BmuI ACTGGG 1 cut(s) 827
BpiI GAAGAC 1 cut(s) 804
BpmI CTGGAG 2 cut(s) 389, 576
Bpu10I CCTNAGC 1 cut(s) 294
BsaJI CCNNGG 1 cut(s) 156
Bsc4I CCNNNNNNNGG 3 cut(s) 40, 331, 865
Bse1I ACTGG 3 cut(s) 593, 714, 822
Bse3DI GCAATG 1 cut(s) 829
BseBI CCWGG 2 cut(s) 21, 158
BseDI CCNNGG 1 cut(s) 156
BseGI GGATG 4 cut(s) 166, 170, 340, 460
BseLI CCNNNNNNNGG 3 cut(s) 40, 331, 865
BseMI GCAATG 1 cut(s) 829
BseMII CTCAG 4 cut(s) 308, 465, 480, 619
BseNI ACTGG 3 cut(s) 593, 714, 822
BseXI GCAGC 1 cut(s) 705
BsiHKAI GWGCWC 1 cut(s) 616
BslI CCNNNNNNNGG 3 cut(s) 40, 331, 865
BsmAI GTCTC 1 cut(s) 288
Bsp1286I GDGCHC 1 cut(s) 616
Bsp143I GATC 5 cut(s) 28, 43, 111, 217, 1035
BspACI CCGC 3 cut(s) 129, 866, 894
BspCNI CTCAG 4 cut(s) 307, 466, 481, 618
BspLI GGNNCC 2 cut(s) 45, 995
BspMI ACCTGC 1 cut(s) 284
BspPI GGATC 3 cut(s) 38, 51, 1043
BspTI CTTAAG 1 cut(s) 520
BsrDI GCAATG 1 cut(s) 829
BsrI ACTGG 3 cut(s) 593, 714, 822
BssECI CCNNGG 1 cut(s) 156
BssMI GATC 5 cut(s) 28, 43, 111, 217, 1035
Bst2UI CCWGG 2 cut(s) 21, 158
Bst4CI ACNGT 5 cut(s) 361, 464, 846, 886, 934
Bst6I CTCTTC 1 cut(s) 19
BstAFI CTTAAG 1 cut(s) 520
BstC8I GCNNGC 1 cut(s) 261
BstDEI CTNAG 4 cut(s) 294, 474, 489, 605
BstEII GGTNACC 1 cut(s) 392
BstF5I GGATG 4 cut(s) 166, 170, 340, 460
BstKTI GATC 5 cut(s) 31, 46, 114, 220, 1038
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 5 cut(s) 28, 43, 111, 217, 1035
BstMWI GCNNNNNNNGC 1 cut(s) 902
BstNI CCWGG 2 cut(s) 21, 158
BstNSI RCATGY 1 cut(s) 283
BstPI GGTNACC 1 cut(s) 392
BstSCI CCNGG 2 cut(s) 19, 156
BstV1I GCAGC 1 cut(s) 705
BstV2I GAAGAC 1 cut(s) 804
BstX2I RGATCY 3 cut(s) 28, 43, 1035
BstYI RGATCY 3 cut(s) 28, 43, 1035
BtsCI GGATG 4 cut(s) 166, 170, 340, 460
BtsIMutI CAGTG 5 cut(s) 357, 600, 613, 930, 982
BveI ACCTGC 1 cut(s) 284
Cac8I GCNNGC 1 cut(s) 261
Cfr13I GGNCC 1 cut(s) 427
Csp6I GTAC 3 cut(s) 178, 481, 1000
CviAII CATG 3 cut(s) 280, 451, 469
CviJI RGCY 9 cut(s) 89, 259, 263, 473, 632, 669, 728, 869, 881
CviKI_1 RGCY 9 cut(s) 89, 259, 263, 473, 632, 669, 728, 869, 881
CviQI GTAC 3 cut(s) 178, 481, 1000
DdeI CTNAG 4 cut(s) 294, 474, 489, 605
DpnI GATC 5 cut(s) 30, 45, 113, 219, 1037
DpnII GATC 5 cut(s) 28, 43, 111, 217, 1035
DraI TTTAAA 1 cut(s) 814
DraIII CACNNNGTG 1 cut(s) 608
DrdI GACNNNNNNGTC 1 cut(s) 390
DseDI GACNNNNNNGTC 1 cut(s) 390
Eam1104I CTCTTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 19
Eco47I GGWCC 1 cut(s) 427
Eco91I GGTNACC 1 cut(s) 392
EcoO65I GGTNACC 1 cut(s) 392
EcoRI GAATTC 1 cut(s) 151
EcoRII CCWGG 2 cut(s) 19, 156
FaeI CATG 3 cut(s) 283, 454, 472
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FatI CATG 3 cut(s) 279, 450, 468
FblI GTMKAC 1 cut(s) 382
Fnu4HI GCNGC 3 cut(s) 719, 867, 894
FokI GGATG 4 cut(s) 173, 177, 347, 467
Fsp4HI GCNGC 3 cut(s) 719, 867, 894
FspBI CTAG 4 cut(s) 203, 287, 341, 639
GluI GCNGC 3 cut(s) 719, 867, 894
GsuI CTGGAG 2 cut(s) 389, 576
Hin1II CATG 3 cut(s) 283, 454, 472
HindIII AAGCTT 1 cut(s) 667
HinfI GANTC 3 cut(s) 206, 384, 409
HphI GGTGA 3 cut(s) 118, 386, 476
Hpy166II GTNNAC 1 cut(s) 383
Hpy188I TCNGA 8 cut(s) 35, 187, 226, 508, 692, 831, 840, 1014
Hpy188III TCNNGA 5 cut(s) 104, 145, 266, 287, 368
Hpy8I GTNNAC 1 cut(s) 383
HpyAV CCTTC 4 cut(s) 72, 72, 407, 883
HpyCH4III ACNGT 5 cut(s) 361, 464, 846, 886, 934
HpyCH4V TGCA 5 cut(s) 195, 279, 310, 404, 625
HpyF10VI GCNNNNNNNGC 1 cut(s) 902
HpyF3I CTNAG 4 cut(s) 294, 474, 489, 605
Hsp92II CATG 3 cut(s) 283, 454, 472
Kzo9I GATC 5 cut(s) 28, 43, 111, 217, 1035
LmnI GCTCC 5 cut(s) 256, 370, 586, 611, 861
Lsp1109I GCAGC 1 cut(s) 705
LweI GCATC 3 cut(s) 280, 353, 750
MaeI CTAG 4 cut(s) 203, 287, 341, 639
MaeIII GTNAC 6 cut(s) 392, 431, 464, 524, 600, 898
MalI GATC 5 cut(s) 30, 45, 113, 219, 1037
MboI GATC 5 cut(s) 28, 43, 111, 217, 1035
MboII GAAGA 3 cut(s) 36, 505, 809
MfeI CAATTG 3 cut(s) 564, 620, 958
MflI RGATCY 3 cut(s) 28, 43, 1035
MhlI GDGCHC 1 cut(s) 616
MluCI AATT 7 cut(s) 151, 188, 564, 620, 685, 919, 958
MlyI GAGTC 3 cut(s) 215, 378, 403
MmeI TCCRAC 1 cut(s) 58
MnlI CCTC 4 cut(s) 130, 502, 550, 937
MseI TTAA 4 cut(s) 363, 521, 807, 813
MspA1I CMGCKG 3 cut(s) 131, 866, 896
MspCI CTTAAG 1 cut(s) 520
MspR9I CCNGG 2 cut(s) 21, 158
MunI CAATTG 3 cut(s) 564, 620, 958
MvaI CCWGG 2 cut(s) 21, 158
MwoI GCNNNNNNNGC 1 cut(s) 902
NdeII GATC 5 cut(s) 28, 43, 111, 217, 1035
NlaIII CATG 3 cut(s) 283, 454, 472
NlaIV GGNNCC 2 cut(s) 45, 995
NmuCI GTSAC 5 cut(s) 392, 431, 464, 524, 600
NspI RCATGY 1 cut(s) 283
PkrI GCNGC 3 cut(s) 720, 868, 895
PleI GAGTC 3 cut(s) 214, 378, 403
PpsI GAGTC 3 cut(s) 214, 378, 403
Psp6I CCWGG 2 cut(s) 19, 156
PspEI GGTNACC 1 cut(s) 392
PspGI CCWGG 2 cut(s) 19, 156
PspN4I GGNNCC 2 cut(s) 45, 995
PspPI GGNCC 1 cut(s) 427
PsuI RGATCY 3 cut(s) 28, 43, 1035
RsaI GTAC 3 cut(s) 179, 482, 1001
RsaNI GTAC 3 cut(s) 178, 481, 1000
SaqAI TTAA 4 cut(s) 363, 521, 807, 813
SatI GCNGC 3 cut(s) 719, 867, 894
Sau3AI GATC 5 cut(s) 28, 43, 111, 217, 1035
Sau96I GGNCC 1 cut(s) 427
SchI GAGTC 3 cut(s) 215, 378, 403
ScrFI CCNGG 2 cut(s) 21, 158
SduI GDGCHC 1 cut(s) 616
SfaNI GCATC 3 cut(s) 280, 353, 750
SinI GGWCC 1 cut(s) 427
SmlI CTYRAG 1 cut(s) 520
SmoI CTYRAG 1 cut(s) 520
SpeI ACTAGT 1 cut(s) 638
Sse9I AATT 7 cut(s) 151, 188, 564, 620, 685, 919, 958
SsiI CCGC 3 cut(s) 129, 866, 894
SspI AATATT 1 cut(s) 72
SspMI CTAG 4 cut(s) 203, 287, 341, 639
StyD4I CCNGG 2 cut(s) 19, 156
TaaI ACNGT 5 cut(s) 361, 464, 846, 886, 934
TaqI TCGA 3 cut(s) 240, 744, 939
TasI AATT 7 cut(s) 151, 188, 564, 620, 685, 919, 958
TatI WGTACW 2 cut(s) 177, 999
TauI GCSGC 2 cut(s) 869, 896
Tru1I TTAA 4 cut(s) 363, 521, 807, 813
Tru9I TTAA 4 cut(s) 363, 521, 807, 813
TscAI CASTG 5 cut(s) 364, 600, 613, 937, 982
TseFI GTSAC 5 cut(s) 392, 431, 464, 524, 600
TseI GCWGC 1 cut(s) 718
Tsp45I GTSAC 5 cut(s) 392, 431, 464, 524, 600
TspDTI ATGAA 5 cut(s) 43, 164, 351, 669, 932
TspGWI ACGGA 1 cut(s) 419
TspRI CASTG 5 cut(s) 364, 600, 613, 937, 982
Vha464I CTTAAG 1 cut(s) 520
VpaK11BI GGWCC 1 cut(s) 427
XapI RAATTY 2 cut(s) 151, 919
XbaI TCTAGA 1 cut(s) 286
XceI RCATGY 1 cut(s) 283
XmiI GTMKAC 1 cut(s) 382
XspI CTAG 4 cut(s) 203, 287, 341, 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.