Rorug04G0308600

magnesium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004962
Physical Location & Seq
Reverse (-)
48400070 .. 48401776
1707 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0308600.1

Sequence Viewer

Length: 1413 bp
ATGGCTGGCACCGGATGGATTATTCAAATTAATGAAGATCTCAAGAACATGGAAGGTCTTTCAACAGAAAAGGATCGCTGGAATAAGGGGTCAATCTACAAACTACCAGCTAGCCTCACAGACATGAACAAGAAGGCCTACAAGCCTCAAACAGTCTCCTTTGGGCCTTACCACTACAATTCCTTAAATCCAACGGAGGAGCACAAGCACCGCGCGCTGCTTCATTTCCTCAAAAGATGTGGAAAGCCTGTTGAGTTGTTTGTAGATGCATTGGCTAAAGTAGAGAATGAATTGAAGGATTCATATACTCTGCTTCGTTCTGTGCCGAAAGAAATTACGGACATATTCCTGCAACTGATGATTTTAGATGGCTGTTTCATGCTGGAAATCATGAGGAGTGCTGCTCATGTACTGGGTGATTATGCTCCTAATGATCCCATCTTTGGAGAACATGGTAGGCTCCATGTCGTGCCATACATCAAGCGTGACATGTTAATGCTTGAGAATCAGTTGCCAATGCTTGTTCTTGAAAAGCTGGTGGCAGTTGAACATGATAAAGCTAAGGATGAAGGGTTTGTGACCAAGCTAATAGTCAAGTTCCTCTGCTCTAGTAACGTTTCTCCAAACATGGGCAAGTGTGTGCATGTGTTGGACATGTACAGGAAGAGTCTGCTTCAGCATGAGCCTGATCCCAAATATATTCGCTACAAAATAAGGTCAAGTGAGCATGACGATGAGATCATCTGGTCCGCAACAGCGCTCAATGAAGCAGGAATCAGGTTCAAGAAAAGTGCAACTACAAGCCTCAAAGACATCACATTCGCCCGGGGAGTGTTAAGGCTCCCTGTTGTTGTTGTGGATGACACCACCGAGTCCATGTTCTTGAATCTGATGGCGTTTGAAAGATTCCATGTAGGAGCAGGAAATGAGGTGACATCTTATTTACTCTTCATGGACAACATCATAGACAATGCTATGGATGTTGCCCTCTTACACTCGAAAGGGATCATCCAGAATGCTTTGGGGAGTGACAAAGCAGTGGCTAATCTGTTCAATTCACTGTCTAAGGATGTGACACAGGACCCGGATAGCAGCCTTCAAATTGTGCATAAGAGGGTGTATCACTACTGCAGAAAGCCGTGGAATGAGTGGCGCGCCAATCTTATTCACACTTACTTCCGAAATCCATGGGCTATTATCTCTGTCATTGCTGGTGTCTTCCTCTTTGTGTTCACTATAGCTCAGACTGGATATGCCATATATCCCTATTACCATCCACCAAATGATTCTTCTCCTTCCACTGTCATTCTCGTTCCAGCTCCTCCAAAACATTCATCCTCCGACTCCACCTCCCCATCGACACTTGTGATTTCTTTCATCCTTGTGACCATGGTATGCATGCTGACCAATTGA

Protein Analysis

470

Amino Acids

53.26

Weight (kDa)

7.01

Isoelectric Point (pI)

37.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 32 - 410 1.7e-114 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 8
AccII CGCG 3 cut(s) 213, 215, 1155
AciI CCGC 2 cut(s) 211, 750
AclI AACGTT 1 cut(s) 615
AclWI GGATC 4 cut(s) 81, 428, 683, 1013
AcuI CTGAAG 1 cut(s) 659
AfaI GTAC 2 cut(s) 411, 659
AfeI AGCGCT 1 cut(s) 759
AfiI CCNNNNNNNGG 2 cut(s) 443, 629
AflIII ACRYGT 2 cut(s) 489, 654
AjuI GAANNNNNNNTTGG 2 cut(s) 1273, 1305
AluBI AGCT 6 cut(s) 110, 535, 560, 586, 1241, 1319
AluI AGCT 6 cut(s) 110, 535, 560, 586, 1241, 1319
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 1 cut(s) 160
AlwI GGATC 4 cut(s) 81, 428, 683, 1013
Ama87I CYCGRG 1 cut(s) 825
Aor51HI AGCGCT 1 cut(s) 759
AoxI GGCC 2 cut(s) 135, 164
ApeKI GCWGC 3 cut(s) 217, 401, 1092
ArsI GACNNNNNNTTYG 2 cut(s) 803, 835
AscI GGCGCGCC 1 cut(s) 1153
AseI ATTAAT 1 cut(s) 30
AspLEI GCGC 5 cut(s) 215, 217, 760, 1155, 1157
AspS9I GGNCC 3 cut(s) 164, 747, 1081
AsuC2I CCSGG 3 cut(s) 826, 827, 1085
AsuHPI GGTGA 2 cut(s) 428, 943
AsuNHI GCTAGC 1 cut(s) 110
AvaI CYCGRG 1 cut(s) 825
AvaII GGWCC 2 cut(s) 747, 1081
BanI GGYRCC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 1210
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 3 cut(s) 204, 388, 1104
BccI CCATC 6 cut(s) 9, 362, 446, 886, 1281, 1363
BceAI ACGGC 1 cut(s) 1123
BcnI CCSGG 3 cut(s) 826, 827, 1085
BcoDI GTCTC 1 cut(s) 160
BfaI CTAG 2 cut(s) 111, 609
BfmI CTRYAG 2 cut(s) 1129, 1236
BfoI RGCGCY 1 cut(s) 761
BglII AGATCT 1 cut(s) 37
BisI GCNGC 3 cut(s) 218, 402, 1093
BlsI GCNGC 3 cut(s) 219, 403, 1094
Bme1390I CCNGG 3 cut(s) 826, 827, 1085
Bme18I GGWCC 2 cut(s) 747, 1081
BmeT110I CYCGRG 1 cut(s) 825
BmgT120I GGNCC 3 cut(s) 164, 747, 1081
BmiI GGNNCC 4 cut(s) 10, 461, 842, 1083
BmrFI CCNGG 3 cut(s) 826, 827, 1085
BmrI ACTGGG 1 cut(s) 422
BmsI GCATC 1 cut(s) 256
BmtI GCTAGC 1 cut(s) 114
BmuI ACTGGG 1 cut(s) 422
BpiI GAAGAC 1 cut(s) 1210
BplI GAGNNNNNCTC 2 cut(s) 388, 420
Bpu10I CCTNAGC 1 cut(s) 561
BpuEI CTTGAG 2 cut(s) 26, 521
BpuMI CCSGG 3 cut(s) 826, 827, 1085
BsaJI CCNNGG 5 cut(s) 825, 826, 1139, 1187, 1389
BsaWI WCCGGW 1 cut(s) 11
BsaXI ACNNNNNCTCC 2 cut(s) 1334, 1364
Bsc4I CCNNNNNNNGG 2 cut(s) 443, 629
Bse1I ACTGG 2 cut(s) 417, 1252
Bse3DI GCAATG 1 cut(s) 1206
BseDI CCNNGG 5 cut(s) 825, 826, 1139, 1187, 1389
BseGI GGATG 9 cut(s) 20, 571, 865, 985, 1008, 1075, 1273, 1334, 1377
BseLI CCNNNNNNNGG 2 cut(s) 443, 629
BseMI GCAATG 1 cut(s) 1206
BseMII CTCAG 1 cut(s) 1256
BseNI ACTGG 2 cut(s) 417, 1252
BsePI GCGCGC 2 cut(s) 213, 1153
BseRI GAGGAG 3 cut(s) 212, 409, 1311
BseXI GCAGC 3 cut(s) 204, 388, 1104
Bsh1236I CGCG 3 cut(s) 213, 215, 1155
BshFI GGCC 2 cut(s) 137, 166
BshNI GGYRCC 1 cut(s) 8
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 825
BsiSI CCGG 3 cut(s) 12, 826, 1085
BslI CCNNNNNNNGG 2 cut(s) 443, 629
BsmAI GTCTC 1 cut(s) 160
BsmI GAATGC 1 cut(s) 1021
BsnI GGCC 2 cut(s) 137, 166
BsoBI CYCGRG 1 cut(s) 825
Bsp1286I GDGCHC 1 cut(s) 204
Bsp1407I TGTACA 1 cut(s) 657
Bsp143I GATC 6 cut(s) 37, 73, 433, 688, 738, 1005
Bsp19I CCATGG 2 cut(s) 1187, 1389
BspACI CCGC 2 cut(s) 211, 750
BspANI GGCC 2 cut(s) 137, 166
BspCNI CTCAG 1 cut(s) 1255
BspFNI CGCG 3 cut(s) 213, 215, 1155
BspHI TCATGA 1 cut(s) 390
BspLI GGNNCC 4 cut(s) 10, 461, 842, 1083
BspMAI CTGCAG 1 cut(s) 1133
BspOI GCTAGC 1 cut(s) 114
BspPI GGATC 4 cut(s) 81, 428, 683, 1013
BspT107I GGYRCC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 1206
BsrGI TGTACA 1 cut(s) 657
BsrI ACTGG 2 cut(s) 417, 1252
BssECI CCNNGG 5 cut(s) 825, 826, 1139, 1187, 1389
BssHII GCGCGC 2 cut(s) 213, 1153
BssMI GATC 6 cut(s) 37, 73, 433, 688, 738, 1005
BssT1I CCWWGG 2 cut(s) 1187, 1389
Bst4CI ACNGT 3 cut(s) 154, 1062, 1303
Bst6I CTCTTC 2 cut(s) 659, 953
BstAUI TGTACA 1 cut(s) 657
BstC8I GCNNGC 5 cut(s) 7, 112, 215, 1155, 1400
BstDEI CTNAG 3 cut(s) 561, 1065, 1242
BstDSI CCRYGG 3 cut(s) 1139, 1187, 1389
BstF5I GGATG 9 cut(s) 20, 571, 865, 985, 1008, 1075, 1273, 1334, 1377
BstFNI CGCG 3 cut(s) 213, 215, 1155
BstH2I RGCGCY 1 cut(s) 761
BstHHI GCGC 5 cut(s) 215, 217, 760, 1155, 1157
BstKTI GATC 6 cut(s) 40, 76, 436, 691, 741, 1008
BstMAI GTCTC 1 cut(s) 160
BstMBI GATC 6 cut(s) 37, 73, 433, 688, 738, 1005
BstMWI GCNNNNNNNGC 1 cut(s) 214
BstNSI RCATGY 4 cut(s) 493, 647, 658, 1402
BstSCI CCNGG 3 cut(s) 824, 825, 1083
BstSFI CTRYAG 2 cut(s) 1129, 1236
BstUI CGCG 3 cut(s) 213, 215, 1155
BstV1I GCAGC 3 cut(s) 204, 388, 1104
BstV2I GAAGAC 1 cut(s) 1210
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuRI GGCC 2 cut(s) 137, 166
BtgI CCRYGG 3 cut(s) 1139, 1187, 1389
BtsCI GGATG 9 cut(s) 20, 571, 865, 985, 1008, 1075, 1273, 1334, 1377
BtsI GCAGTG 1 cut(s) 1044
BtsIMutI CAGTG 3 cut(s) 1044, 1058, 1299
Cac8I GCNNGC 5 cut(s) 7, 112, 215, 1155, 1400
CciI TCATGA 1 cut(s) 390
CfoI GCGC 5 cut(s) 215, 217, 760, 1155, 1157
Cfr13I GGNCC 3 cut(s) 164, 747, 1081
Cfr9I CCCGGG 1 cut(s) 825
Csp6I GTAC 2 cut(s) 410, 658
CspCI CAANNNNNGTGG 2 cut(s) 220, 255
CviQI GTAC 2 cut(s) 410, 658
DdeI CTNAG 3 cut(s) 561, 1065, 1242
DpnI GATC 6 cut(s) 39, 75, 435, 690, 740, 1007
DpnII GATC 6 cut(s) 37, 73, 433, 688, 738, 1005
Eam1104I CTCTTC 2 cut(s) 659, 953
EarI CTCTTC 2 cut(s) 659, 953
Eco130I CCWWGG 2 cut(s) 1187, 1389
Eco147I AGGCCT 1 cut(s) 137
Eco47I GGWCC 2 cut(s) 747, 1081
Eco47III AGCGCT 1 cut(s) 759
Eco57I CTGAAG 1 cut(s) 659
Eco88I CYCGRG 1 cut(s) 825
EcoO109I RGGNCCY 1 cut(s) 1081
EcoT14I CCWWGG 2 cut(s) 1187, 1389
EcoT22I ATGCAT 2 cut(s) 271, 1400
ErhI CCWWGG 2 cut(s) 1187, 1389
Fnu4HI GCNGC 3 cut(s) 218, 402, 1093
FokI GGATG 9 cut(s) 27, 578, 872, 992, 995, 1082, 1260, 1321, 1364
Fsp4HI GCNGC 3 cut(s) 218, 402, 1093
FspBI CTAG 2 cut(s) 111, 609
GlaI GCGC 5 cut(s) 214, 216, 759, 1154, 1156
GluI GCNGC 3 cut(s) 218, 402, 1093
HaeII RGCGCY 1 cut(s) 761
HaeIII GGCC 2 cut(s) 137, 166
HapII CCGG 3 cut(s) 12, 826, 1085
HhaI GCGC 5 cut(s) 215, 217, 760, 1155, 1157
Hin6I GCGC 5 cut(s) 213, 215, 758, 1153, 1155
HinP1I GCGC 5 cut(s) 213, 215, 758, 1153, 1155
HinfI GANTC 9 cut(s) 299, 505, 667, 774, 872, 886, 906, 1286, 1343
HpaII CCGG 3 cut(s) 12, 826, 1085
HphI GGTGA 2 cut(s) 428, 943
Hpy166II GTNNAC 1 cut(s) 1233
Hpy188I TCNGA 4 cut(s) 891, 1181, 1245, 1342
Hpy188III TCNNGA 6 cut(s) 43, 391, 527, 784, 883, 1012
Hpy8I GTNNAC 1 cut(s) 1233
HpyAV CCTTC 6 cut(s) 47, 127, 289, 563, 1106, 1305
HpyCH4III ACNGT 3 cut(s) 154, 1062, 1303
HpyCH4IV ACGT 1 cut(s) 615
HpyCH4V TGCA 7 cut(s) 269, 352, 643, 794, 1108, 1131, 1398
HpyF10VI GCNNNNNNNGC 1 cut(s) 214
HpyF3I CTNAG 3 cut(s) 561, 1065, 1242
HpySE526I ACGT 1 cut(s) 615
HspAI GCGC 5 cut(s) 213, 215, 758, 1153, 1155
Kzo9I GATC 6 cut(s) 37, 73, 433, 688, 738, 1005
LmnI GCTCC 6 cut(s) 199, 430, 465, 846, 917, 1324
Lsp1109I GCAGC 3 cut(s) 204, 388, 1104
LweI GCATC 1 cut(s) 256
MaeI CTAG 2 cut(s) 111, 609
MaeII ACGT 1 cut(s) 615
MaeIII GTNAC 7 cut(s) 485, 577, 611, 931, 1028, 1072, 1384
MalI GATC 6 cut(s) 39, 75, 435, 690, 740, 1007
MboI GATC 6 cut(s) 37, 73, 433, 688, 738, 1005
MboII GAAGA 5 cut(s) 47, 676, 940, 1210, 1281
MfeI CAATTG 1 cut(s) 1408
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 204
MluCI AATT 7 cut(s) 27, 178, 290, 333, 1054, 1101, 1408
MlyI GAGTC 3 cut(s) 676, 881, 1337
MmeI TCCRAC 3 cut(s) 215, 630, 1365
Mph1103I ATGCAT 2 cut(s) 271, 1400
MseI TTAA 4 cut(s) 30, 185, 494, 836
MslI CAYNNNNRTG 4 cut(s) 122, 494, 732, 1382
MspI CCGG 3 cut(s) 12, 826, 1085
MspR9I CCNGG 3 cut(s) 826, 827, 1085
MunI CAATTG 1 cut(s) 1408
Mva1269I GAATGC 1 cut(s) 1021
MvnI CGCG 3 cut(s) 213, 215, 1155
MwoI GCNNNNNNNGC 1 cut(s) 214
NciI CCSGG 3 cut(s) 826, 827, 1085
NcoI CCATGG 2 cut(s) 1187, 1389
NdeII GATC 6 cut(s) 37, 73, 433, 688, 738, 1005
NheI GCTAGC 1 cut(s) 110
NlaIV GGNNCC 4 cut(s) 10, 461, 842, 1083
NmuCI GTSAC 6 cut(s) 485, 577, 931, 1028, 1072, 1384
NsiI ATGCAT 2 cut(s) 271, 1400
NspI RCATGY 4 cut(s) 493, 647, 658, 1402
PaeI GCATGC 1 cut(s) 1402
PagI TCATGA 1 cut(s) 390
PalAI GGCGCGCC 1 cut(s) 1153
PauI GCGCGC 2 cut(s) 213, 1153
PceI AGGCCT 1 cut(s) 137
PciI ACATGT 2 cut(s) 489, 654
PctI GAATGC 1 cut(s) 1021
PfeI GAWTC 6 cut(s) 299, 505, 774, 886, 906, 1286
PkrI GCNGC 3 cut(s) 219, 403, 1094
PleI GAGTC 3 cut(s) 675, 880, 1337
PpsI GAGTC 3 cut(s) 675, 880, 1337
PpuMI RGGWCCY 1 cut(s) 1081
PscI ACATGT 2 cut(s) 489, 654
PshBI ATTAAT 1 cut(s) 30
Psp1406I AACGTT 1 cut(s) 615
Psp5II RGGWCCY 1 cut(s) 1081
PspN4I GGNNCC 4 cut(s) 10, 461, 842, 1083
PspPI GGNCC 3 cut(s) 164, 747, 1081
PspPPI RGGWCCY 1 cut(s) 1081
PstI CTGCAG 1 cut(s) 1133
PsuI RGATCY 1 cut(s) 37
PteI GCGCGC 2 cut(s) 213, 1153
RsaI GTAC 2 cut(s) 411, 659
RsaNI GTAC 2 cut(s) 410, 658
RseI CAYNNNNRTG 4 cut(s) 122, 494, 732, 1382
SaqAI TTAA 4 cut(s) 30, 185, 494, 836
SatI GCNGC 3 cut(s) 218, 402, 1093
Sau3AI GATC 6 cut(s) 37, 73, 433, 688, 738, 1005
Sau96I GGNCC 3 cut(s) 164, 747, 1081
SchI GAGTC 3 cut(s) 676, 881, 1337
ScrFI CCNGG 3 cut(s) 826, 827, 1085
SduI GDGCHC 1 cut(s) 204
SfaNI GCATC 1 cut(s) 256
SfcI CTRYAG 2 cut(s) 1129, 1236
SgsI GGCGCGCC 1 cut(s) 1153
SinI GGWCC 2 cut(s) 747, 1081
SmaI CCCGGG 1 cut(s) 827
SmiMI CAYNNNNRTG 4 cut(s) 122, 494, 732, 1382
SmlI CTYRAG 2 cut(s) 41, 500
SmoI CTYRAG 2 cut(s) 41, 500
SphI GCATGC 1 cut(s) 1402
Sse9I AATT 7 cut(s) 27, 178, 290, 333, 1054, 1101, 1408
SseBI AGGCCT 1 cut(s) 137
SsiI CCGC 2 cut(s) 211, 750
SspMI CTAG 2 cut(s) 111, 609
StuI AGGCCT 1 cut(s) 137
StyD4I CCNGG 3 cut(s) 824, 825, 1083
StyI CCWWGG 2 cut(s) 1187, 1389
TaaI ACNGT 3 cut(s) 154, 1062, 1303
TaiI ACGT 1 cut(s) 618
TaqI TCGA 2 cut(s) 998, 1358
TasI AATT 7 cut(s) 27, 178, 290, 333, 1054, 1101, 1408
TatI WGTACW 2 cut(s) 409, 657
TfiI GAWTC 6 cut(s) 299, 505, 774, 886, 906, 1286
Tru1I TTAA 4 cut(s) 30, 185, 494, 836
Tru9I TTAA 4 cut(s) 30, 185, 494, 836
TscAI CASTG 3 cut(s) 1044, 1065, 1306
TseFI GTSAC 6 cut(s) 485, 577, 931, 1028, 1072, 1384
TseI GCWGC 3 cut(s) 217, 401, 1092
Tsp45I GTSAC 6 cut(s) 485, 577, 931, 1028, 1072, 1384
TspGWI ACGGA 2 cut(s) 209, 353
TspMI CCCGGG 1 cut(s) 825
TspRI CASTG 3 cut(s) 1044, 1065, 1306
VpaK11BI GGWCC 2 cut(s) 747, 1081
VspI ATTAAT 1 cut(s) 30
XceI RCATGY 4 cut(s) 493, 647, 658, 1402
XmaI CCCGGG 1 cut(s) 825
XspI CTAG 2 cut(s) 111, 609
Zsp2I ATGCAT 2 cut(s) 271, 1400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.