Rh4DG365500

magnesium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004962
Physical Location & Seq
Forward (+)
58642598 .. 58646263
3666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG365500.1

Sequence Viewer

Length: 1314 bp
ATGGAAGGATCACGGAGTCCTTTAATTCATTCTGATCATCCAGAATCCACCTCCTCTAATAATACTGGAAACTTTCAGTTTTATGCAAATGGAATTCGTGAGTGTCCTGGTGGTATCCAGGGACTGGAGAAGAGAGGTCATGGAATTCGTGGTTGGCCTGGTGGTATCGAGGAACTGAAGAAGAGAGGTCATGGAAATCGATCTTGGATAAAGATTGATACAACTGGGGACTCAAAGGTTTTGGAGCTTGACAAGGCTACTATTATGAGACATTGTTCTTTGCCTGCCAGAGATCTCCGACTTTTGGACCCTTTGTTCATTTACCCTTCTACAATTTTAGGAAGGGAGAAAGCTATTGTTGTTAGTCTAGAACAGATAAGGTGTATAATCACAGCTGAAGAGGTTATCCTTATGAATTCCCTGGATCGATGTGTTGTTCAGTACAAATCTGAATTATGCAAACGCCTTCAGGCAAACAAAGATCAGTCTGATGACTTGCCCTTCGAATTTAGAGCACTGGAGCTGGCTTTGGAACTAACTTGCTCATCTCTAGATGCTCAGGTAAAAGAACTGGAACTGGAGATATACCCTGTGCTGGATGAACTAGCAACATCCATTAACACTCTGAATCTAGAGCGTGTGCGTAGACTCAAAGGTCACCTCCTTGCCTTGACACAACGAGTGCAGAAGGTCCGTGATGAAATAGAACATCTCATGGATGATGACGGCGACATGGCTGAGATGTACCTGACTGAGAAGAAACAAAGGTCGGAGTGTTTTCCCTCAAGTGACCTTAGCTTTCAAACGAACTCCTCGGGTGATGATAAAGTGGTTTCGAAATCTGCTCCTGTTTCACCAGTAGAGTCACTCGGTCGATCACAGAATTTGCGAAGGACTTTTAGCAGTATTGTGAATTCAAGCAGAACTGGAAGCTTCATTAGTTCATCTAATAGTGAAGAAGACGTTGATCAACTGGAAATGCTGCTTGAAGCATACTTTATTATCATTGACCATACTCTCAGCAAATTGTTATCGCTAAAAGAGTACATTGATGATACTGAAGATTTTATTAACATCAAACTGGGCAATGTTCAGAACCATCTGATAAAATTTGAGTTGCTTCTTACAGCAGCTACCTTTGTGGCTACAATGTTTGCTTCTGTTACAGCAGTATTTGGAATGAATTTTGCAGACTCTGTTTTTGATAATCCATCCACATTTAGTTGGGTTCTGATTATTTGTGGTTTCGTCTGTGGGTTCTTGTACTTCGCTTTCCTGATTTATTGTAGGCACAAAAGGATCTTTCCATTGTAA

Protein Analysis

437

Amino Acids

49.3

Weight (kDa)

5.36

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRS2-like PF22099 75 - 262 6.6e-32 Magnesium transporter MRS2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 654
AccB7I CCANNNNNTGG 1 cut(s) 124
AccI GTMKAC 1 cut(s) 646
AclWI GGATC 3 cut(s) 16, 432, 1307
AcsI RAATTY 8 cut(s) 93, 144, 415, 506, 883, 913, 1109, 1183
AcuI CTGAAG 4 cut(s) 197, 417, 452, 1080
AfaI GTAC 4 cut(s) 443, 746, 1046, 1265
AfiI CCNNNNNNNGG 3 cut(s) 124, 304, 595
AgsI TTSAA 3 cut(s) 803, 918, 989
AjnI CCWGG 4 cut(s) 106, 117, 157, 420
AjuI GAANNNNNNNTTGG 4 cut(s) 136, 168, 187, 219
AloI GAACNNNNNNTCC 2 cut(s) 299, 331
AluBI AGCT 7 cut(s) 247, 353, 395, 523, 798, 933, 1133
AluI AGCT 7 cut(s) 247, 353, 395, 523, 798, 933, 1133
Alw21I GWGCWC 1 cut(s) 517
Alw26I GTCTC 1 cut(s) 262
AlwI GGATC 3 cut(s) 16, 432, 1307
AlwNI CAGNNNCTG 2 cut(s) 124, 1196
Ama87I CYCGRG 1 cut(s) 814
AoxI GGCC 1 cut(s) 155
ApeKI GCWGC 2 cut(s) 982, 1130
ApoI RAATTY 8 cut(s) 93, 144, 415, 506, 883, 913, 1109, 1183
ArsI GACNNNNNNTTYG 4 cut(s) 485, 517, 1184, 1216
AspS9I GGNCC 2 cut(s) 307, 691
AsuHPI GGTGA 3 cut(s) 650, 830, 846
AsuII TTCGAA 2 cut(s) 504, 836
AvaI CYCGRG 1 cut(s) 814
AvaII GGWCC 2 cut(s) 307, 691
BbsI GAAGAC 1 cut(s) 966
Bbv12I GWGCWC 1 cut(s) 517
BbvI GCAGC 2 cut(s) 969, 1142
BccI CCATC 2 cut(s) 1107, 1219
BceAI ACGGC 1 cut(s) 742
BciT130I CCWGG 4 cut(s) 108, 119, 159, 422
BciVI GTATCC 1 cut(s) 125
BclI TGATCA 2 cut(s) 34, 967
BcoDI GTCTC 1 cut(s) 262
BfaI CTAG 4 cut(s) 368, 551, 605, 632
BfuI GTATCC 1 cut(s) 125
BglII AGATCT 1 cut(s) 292
BisI GCNGC 2 cut(s) 983, 1131
BlsI GCNGC 2 cut(s) 984, 1132
Bme1390I CCNGG 4 cut(s) 108, 119, 159, 422
Bme18I GGWCC 2 cut(s) 307, 691
BmeT110I CYCGRG 1 cut(s) 814
BmgT120I GGNCC 2 cut(s) 307, 691
BmiI GGNNCC 1 cut(s) 309
BmrFI CCNGG 4 cut(s) 108, 119, 159, 422
BmrI ACTGGG 2 cut(s) 234, 1091
BmsI GCATC 1 cut(s) 544
BmuI ACTGGG 2 cut(s) 234, 1091
BpiI GAAGAC 1 cut(s) 966
BpmI CTGGAG 3 cut(s) 146, 539, 599
Bpu10I CCTNAGC 2 cut(s) 558, 794
Bpu14I TTCGAA 2 cut(s) 504, 836
BpuEI CTTGAG 1 cut(s) 769
Bsa29I ATCGAT 2 cut(s) 199, 427
BsaJI CCNNGG 3 cut(s) 118, 420, 813
Bsc4I CCNNNNNNNGG 3 cut(s) 124, 304, 595
Bse3DI GCAATG 1 cut(s) 1093
BseBI CCWGG 4 cut(s) 108, 119, 159, 422
BseCI ATCGAT 2 cut(s) 199, 427
BseDI CCNNGG 3 cut(s) 118, 420, 813
BseGI GGATG 5 cut(s) 37, 604, 611, 724, 1211
BseLI CCNNNNNNNGG 3 cut(s) 124, 304, 595
BseMI GCAATG 1 cut(s) 1093
BseMII CTCAG 4 cut(s) 572, 729, 744, 1033
BseRI GAGGAG 2 cut(s) 43, 802
BseXI GCAGC 2 cut(s) 969, 1142
BsgI GTGCAG 1 cut(s) 704
Bsh1285I CGRYCG 1 cut(s) 874
BshFI GGCC 1 cut(s) 157
BshVI ATCGAT 2 cut(s) 199, 427
BsiEI CGRYCG 1 cut(s) 874
BsiHKAI GWGCWC 1 cut(s) 517
BsiHKCI CYCGRG 1 cut(s) 814
BslFI GGGAC 2 cut(s) 135, 242
BslI CCNNNNNNNGG 3 cut(s) 124, 304, 595
BsmAI GTCTC 1 cut(s) 262
BsmFI GGGAC 2 cut(s) 135, 242
BsnI GGCC 1 cut(s) 157
BsoBI CYCGRG 1 cut(s) 814
Bsp119I TTCGAA 2 cut(s) 504, 836
Bsp1286I GDGCHC 1 cut(s) 517
Bsp143I GATC 9 cut(s) 8, 34, 200, 292, 424, 481, 875, 967, 1299
BspANI GGCC 1 cut(s) 157
BspCNI CTCAG 4 cut(s) 571, 730, 745, 1032
BspDI ATCGAT 2 cut(s) 199, 427
BspLI GGNNCC 1 cut(s) 309
BspPI GGATC 3 cut(s) 16, 432, 1307
BspT104I TTCGAA 2 cut(s) 504, 836
BsrDI GCAATG 1 cut(s) 1093
BssECI CCNNGG 3 cut(s) 118, 420, 813
BssMI GATC 9 cut(s) 8, 34, 200, 292, 424, 481, 875, 967, 1299
Bst2UI CCWGG 4 cut(s) 108, 119, 159, 422
Bst6I CTCTTC 3 cut(s) 125, 176, 393
BstBI TTCGAA 2 cut(s) 504, 836
BstC8I GCNNGC 2 cut(s) 285, 525
BstDEI CTNAG 5 cut(s) 558, 738, 753, 794, 1019
BstEII GGTNACC 1 cut(s) 656
BstF5I GGATG 5 cut(s) 37, 604, 611, 724, 1211
BstKTI GATC 9 cut(s) 11, 37, 203, 295, 427, 484, 878, 970, 1302
BstMAI GTCTC 1 cut(s) 262
BstMBI GATC 9 cut(s) 8, 34, 200, 292, 424, 481, 875, 967, 1299
BstMCI CGRYCG 1 cut(s) 874
BstNI CCWGG 4 cut(s) 108, 119, 159, 422
BstPI GGTNACC 1 cut(s) 656
BstSCI CCNGG 4 cut(s) 106, 117, 157, 420
BstV1I GCAGC 2 cut(s) 969, 1142
BstV2I GAAGAC 1 cut(s) 966
BstX2I RGATCY 2 cut(s) 292, 1299
BstYI RGATCY 2 cut(s) 292, 1299
Bsu15I ATCGAT 2 cut(s) 199, 427
BsuI GTATCC 1 cut(s) 125
BsuRI GGCC 1 cut(s) 157
BsuTUI ATCGAT 2 cut(s) 199, 427
BtsCI GGATG 5 cut(s) 37, 604, 611, 724, 1211
BtsIMutI CAGTG 1 cut(s) 515
Cac8I GCNNGC 2 cut(s) 285, 525
CaiI CAGNNNCTG 2 cut(s) 124, 1196
Cfr13I GGNCC 2 cut(s) 307, 691
ClaI ATCGAT 2 cut(s) 199, 427
Csp6I GTAC 4 cut(s) 442, 745, 1045, 1264
CviAII CATG 4 cut(s) 140, 191, 715, 733
CviQI GTAC 4 cut(s) 442, 745, 1045, 1264
DdeI CTNAG 5 cut(s) 558, 738, 753, 794, 1019
DpnI GATC 9 cut(s) 10, 36, 202, 294, 426, 483, 877, 969, 1301
DpnII GATC 9 cut(s) 8, 34, 200, 292, 424, 481, 875, 967, 1299
DrdI GACNNNNNNGTC 1 cut(s) 654
DseDI GACNNNNNNGTC 1 cut(s) 654
Eam1104I CTCTTC 3 cut(s) 125, 176, 393
EarI CTCTTC 3 cut(s) 125, 176, 393
Eco47I GGWCC 2 cut(s) 307, 691
Eco57I CTGAAG 4 cut(s) 197, 417, 452, 1080
Eco88I CYCGRG 1 cut(s) 814
Eco91I GGTNACC 1 cut(s) 656
EcoO65I GGTNACC 1 cut(s) 656
EcoRI GAATTC 4 cut(s) 93, 144, 415, 913
EcoRII CCWGG 4 cut(s) 106, 117, 157, 420
FaeI CATG 4 cut(s) 143, 194, 718, 736
FaqI GGGAC 2 cut(s) 135, 242
FatI CATG 4 cut(s) 139, 190, 714, 732
FbaI TGATCA 2 cut(s) 34, 967
FblI GTMKAC 1 cut(s) 646
Fnu4HI GCNGC 2 cut(s) 983, 1131
FokI GGATG 5 cut(s) 24, 598, 611, 731, 1198
Fsp4HI GCNGC 2 cut(s) 983, 1131
FspBI CTAG 4 cut(s) 368, 551, 605, 632
GluI GCNGC 2 cut(s) 983, 1131
GsuI CTGGAG 3 cut(s) 146, 539, 599
HaeIII GGCC 1 cut(s) 157
Hin1II CATG 4 cut(s) 143, 194, 718, 736
HindIII AAGCTT 1 cut(s) 931
HinfI GANTC 7 cut(s) 16, 44, 230, 628, 648, 863, 1193
HphI GGTGA 3 cut(s) 650, 830, 846
Hpy166II GTNNAC 1 cut(s) 647
Hpy188I TCNGA 9 cut(s) 34, 299, 451, 490, 627, 772, 1095, 1104, 1233
Hpy188III TCNNGA 6 cut(s) 41, 98, 368, 551, 632, 1276
Hpy8I GTNNAC 1 cut(s) 647
HpyAV CCTTC 6 cut(s) 336, 336, 476, 511, 682, 885
HpyCH4IV ACGT 1 cut(s) 963
HpyCH4V TGCA 4 cut(s) 86, 459, 685, 1190
HpyF3I CTNAG 5 cut(s) 558, 738, 753, 794, 1019
HpySE526I ACGT 1 cut(s) 963
Hsp92II CATG 4 cut(s) 143, 194, 718, 736
Ksp22I TGATCA 2 cut(s) 34, 967
Kzo9I GATC 9 cut(s) 8, 34, 200, 292, 424, 481, 875, 967, 1299
LmnI GCTCC 3 cut(s) 244, 520, 850
Lsp1109I GCAGC 2 cut(s) 969, 1142
LweI GCATC 1 cut(s) 544
MaeI CTAG 4 cut(s) 368, 551, 605, 632
MaeII ACGT 1 cut(s) 963
MaeIII GTNAC 4 cut(s) 656, 788, 864, 1162
MalI GATC 9 cut(s) 10, 36, 202, 294, 426, 483, 877, 969, 1301
MboI GATC 9 cut(s) 8, 34, 200, 292, 424, 481, 875, 967, 1299
MboII GAAGA 8 cut(s) 142, 190, 193, 410, 769, 968, 971, 1073
MflI RGATCY 2 cut(s) 292, 1299
MhlI GDGCHC 1 cut(s) 517
MlyI GAGTC 5 cut(s) 25, 224, 642, 872, 1187
MmeI TCCRAC 2 cut(s) 322, 750
MnlI CCTC 9 cut(s) 61, 64, 128, 163, 179, 394, 671, 793, 823
MseI TTAA 3 cut(s) 23, 618, 1071
MspA1I CMGCKG 1 cut(s) 395
MspR9I CCNGG 4 cut(s) 108, 119, 159, 422
MvaI CCWGG 4 cut(s) 108, 119, 159, 422
NdeII GATC 9 cut(s) 8, 34, 200, 292, 424, 481, 875, 967, 1299
NlaIII CATG 4 cut(s) 143, 194, 718, 736
NlaIV GGNNCC 1 cut(s) 309
NmuCI GTSAC 3 cut(s) 656, 788, 864
NspV TTCGAA 2 cut(s) 504, 836
PfeI GAWTC 2 cut(s) 44, 628
PflMI CCANNNNNTGG 1 cut(s) 124
PkrI GCNGC 2 cut(s) 984, 1132
PleI GAGTC 5 cut(s) 24, 224, 642, 871, 1187
PpsI GAGTC 5 cut(s) 24, 224, 642, 871, 1187
Psp6I CCWGG 4 cut(s) 106, 117, 157, 420
PspEI GGTNACC 1 cut(s) 656
PspGI CCWGG 4 cut(s) 106, 117, 157, 420
PspN4I GGNNCC 1 cut(s) 309
PspPI GGNCC 2 cut(s) 307, 691
PstNI CAGNNNCTG 2 cut(s) 124, 1196
PsuI RGATCY 2 cut(s) 292, 1299
PvuII CAGCTG 1 cut(s) 395
RsaI GTAC 4 cut(s) 443, 746, 1046, 1265
RsaNI GTAC 4 cut(s) 442, 745, 1045, 1264
SaqAI TTAA 3 cut(s) 23, 618, 1071
SatI GCNGC 2 cut(s) 983, 1131
Sau3AI GATC 9 cut(s) 8, 34, 200, 292, 424, 481, 875, 967, 1299
Sau96I GGNCC 2 cut(s) 307, 691
SchI GAGTC 5 cut(s) 25, 224, 642, 872, 1187
ScrFI CCNGG 4 cut(s) 108, 119, 159, 422
SduI GDGCHC 1 cut(s) 517
SfaNI GCATC 1 cut(s) 544
SfuI TTCGAA 2 cut(s) 504, 836
SinI GGWCC 2 cut(s) 307, 691
SmlI CTYRAG 1 cut(s) 784
SmoI CTYRAG 1 cut(s) 784
SspMI CTAG 4 cut(s) 368, 551, 605, 632
StyD4I CCNGG 4 cut(s) 106, 117, 157, 420
TaiI ACGT 1 cut(s) 966
TaqI TCGA 6 cut(s) 168, 199, 427, 504, 836, 874
TaqII GACCGA 1 cut(s) 860
TatI WGTACW 3 cut(s) 441, 1044, 1263
TfiI GAWTC 2 cut(s) 44, 628
Tru1I TTAA 3 cut(s) 23, 618, 1071
Tru9I TTAA 3 cut(s) 23, 618, 1071
TscAI CASTG 1 cut(s) 522
TseFI GTSAC 3 cut(s) 656, 788, 864
TseI GCWGC 2 cut(s) 982, 1130
Tsp45I GTSAC 3 cut(s) 656, 788, 864
TspDTI ATGAA 8 cut(s) 17, 307, 428, 615, 714, 925, 933, 1196
TspGWI ACGGA 2 cut(s) 28, 683
TspRI CASTG 1 cut(s) 522
Van91I CCANNNNNTGG 1 cut(s) 124
VpaK11BI GGWCC 2 cut(s) 307, 691
XapI RAATTY 8 cut(s) 93, 144, 415, 506, 883, 913, 1109, 1183
XbaI TCTAGA 3 cut(s) 367, 550, 631
XmiI GTMKAC 1 cut(s) 646
XspI CTAG 4 cut(s) 368, 551, 605, 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.