FvH4_4g30000

magnesium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004962
Physical Location & Seq
Forward (+)
29859550 .. 29863187
3638 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30000.t2

Sequence Viewer

Length: 1320 bp
ATGGATAGACCACAAAGTCCTTTAATTCAATTTTACCATCCAGAGTCCACCTCATCTAATAATACCGGAAGCGTTCGGTTTAATGCAAATGGAATTCGTGGTTGGGCTGGTGGTGTCCAGGGACTGAAGAAGAGAGGTGATGGGATTCGTGGTCGGCCAGGTGGTATTGAGGGACTGAAAAAGAGAGGTCATGTAAATCGTTCGTGGATAAAGATTGATACAATGGGGGAGTCAAAGATTTTGGAGCTTGACAAGGCTACTATTATGAGACACTGTTCTTTGCCTGCCAGAGATATCCGACTTTTGGACCCTTTGTTCATTTACCCTTCTTCAATTTTAGGAAGGGAGAAAGCTATTGTTGTTAGTCTTGAACAGATAAGGTGTATAATCACAGCTGAAGAGGTTATCCTTATGAATTCCCTGGATCGATGTGTTCTTCAGTACAATTCTGAATTATGCAAACGCCTTCAGGCAAACAAAGATCATTCTGATGACTTGCCCTTCGAATTTAGAGCACTGGAGCTGGCTTTGGAACTAACTTGCATATCTCTAGATGCTCAGGTAAGAGAACTCGAACTGGAGATATACCCGGTGCTGGATGAACTAGCAACATCCATTAACACTCTGAATCTAGAGCGTGTGCGTAGACTCAAAGGTCACCTCCTTGCCTTGACACAACGAGTGCAGAAGGTCCGTGATGAAATAGAACAACTCATGGATGACGACGGCGACATGGCTGAGATGTACCTGACTGAGAAGAAACAAAGATCGGAGTGTTTTCCCTTGACCTTAAGTGACCTTGGTTCTCAAACTAACTCCTCAGGTGATGAAAAAGTGGTTTCAAAATCTGCTCCTGTTTCACCAGTAGAGTCAATCAATGGATCACAGAATTTGCAAAGGGCTTTTAGCGGTACTGTGAATTCAAGCAGAACTGGAAGCTTAATTAATTCATCTGACAGTGAAGAAAACATTGATCAGCTGGAAATGCTGCTTGAAGCATACTTTGTTGTCATTGACCATACTCTCAGCAAATTGCTTTCGCTAAAAGAATACATTGACGATACTGAAGATTTTATTAACATCAAACTGGGCAATGTTCAGAACCATCTGATAAAATTTGAGTTGCTTCTTACAGCAGCTACCTTTGTGGCTACAATGTTTGCATCTGTTACAGCAGTATTCGGAATGAATTTTGCAGCCTCTGTTTTTCAAGATCCATCTTCATTCAGTTGGGTTTTGATTATTTGTGGTATTGTCTGTGGGTTCTTGTACTTTGCTTTCCTGATTTATTGTAGGCACAAAAGGATATTTCCATTGTAA

Protein Analysis

440

Amino Acids

49.28

Weight (kDa)

5.54

Isoelectric Point (pI)

42.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRS2-like PF22099 81 - 257 8.1e-29 Magnesium transporter MRS2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 15, 654
AccI GTMKAC 1 cut(s) 646
AciI CCGC 1 cut(s) 909
AclWI GGATC 3 cut(s) 432, 889, 1208
AcoI YGGCCR 1 cut(s) 155
AcsI RAATTY 7 cut(s) 93, 415, 506, 889, 919, 1115, 1189
AcuI CTGAAG 5 cut(s) 146, 417, 422, 452, 1086
AfaI GTAC 4 cut(s) 443, 746, 913, 1271
AfiI CCNNNNNNNGG 2 cut(s) 304, 595
AflII CTTAAG 1 cut(s) 790
AgsI TTSAA 7 cut(s) 29, 333, 371, 843, 924, 995, 1211
AjnI CCWGG 3 cut(s) 117, 157, 420
AjuI GAANNNNNNNTTGG 2 cut(s) 85, 117
AloI GAACNNNNNNTCC 2 cut(s) 299, 331
AluBI AGCT 7 cut(s) 247, 353, 395, 523, 939, 979, 1139
AluI AGCT 7 cut(s) 247, 353, 395, 523, 939, 979, 1139
Alw21I GWGCWC 1 cut(s) 517
Alw26I GTCTC 1 cut(s) 262
AlwI GGATC 3 cut(s) 432, 889, 1208
AlwNI CAGNNNCTG 2 cut(s) 124, 1202
AoxI GGCC 1 cut(s) 155
ApeKI GCWGC 3 cut(s) 988, 1136, 1196
ApoI RAATTY 7 cut(s) 93, 415, 506, 889, 919, 1115, 1189
ArsI GACNNNNNNTTYG 2 cut(s) 485, 517
AseI ATTAAT 1 cut(s) 945
AspS9I GGNCC 2 cut(s) 307, 691
AsuC2I CCSGG 1 cut(s) 590
AsuHPI GGTGA 4 cut(s) 149, 650, 836, 852
AsuII TTCGAA 1 cut(s) 504
AvaII GGWCC 2 cut(s) 307, 691
AxyI CCTNAGG 1 cut(s) 820
Bbv12I GWGCWC 1 cut(s) 517
BbvI GCAGC 3 cut(s) 975, 1148, 1208
BccI CCATC 4 cut(s) 45, 134, 1113, 1225
BceAI ACGGC 1 cut(s) 742
BciT130I CCWGG 3 cut(s) 119, 159, 422
BclI TGATCA 1 cut(s) 973
BcnI CCSGG 1 cut(s) 590
BcoDI GTCTC 1 cut(s) 262
BfaI CTAG 3 cut(s) 551, 605, 632
BfrI CTTAAG 1 cut(s) 790
BisI GCNGC 3 cut(s) 989, 1137, 1197
BlsI GCNGC 3 cut(s) 990, 1138, 1198
Bme1390I CCNGG 4 cut(s) 119, 159, 422, 590
Bme18I GGWCC 2 cut(s) 307, 691
BmgT120I GGNCC 2 cut(s) 307, 691
BmiI GGNNCC 1 cut(s) 309
BmrFI CCNGG 4 cut(s) 119, 159, 422, 590
BmrI ACTGGG 1 cut(s) 1097
BmsI GCATC 2 cut(s) 544, 1172
BmuI ACTGGG 1 cut(s) 1097
BplI GAGNNNNNCTC 2 cut(s) 35, 67
BpmI CTGGAG 2 cut(s) 539, 599
Bpu10I CCTNAGC 1 cut(s) 558
Bpu14I TTCGAA 1 cut(s) 504
BpuMI CCSGG 1 cut(s) 590
Bsa29I ATCGAT 1 cut(s) 427
BsaJI CCNNGG 3 cut(s) 118, 420, 799
BsaWI WCCGGW 1 cut(s) 65
Bsc4I CCNNNNNNNGG 2 cut(s) 304, 595
Bse1I ACTGG 5 cut(s) 522, 582, 863, 937, 1092
Bse21I CCTNAGG 1 cut(s) 820
Bse3DI GCAATG 1 cut(s) 1099
BseBI CCWGG 3 cut(s) 119, 159, 422
BseCI ATCGAT 1 cut(s) 427
BseDI CCNNGG 3 cut(s) 118, 420, 799
BseGI GGATG 4 cut(s) 37, 604, 611, 724
BseLI CCNNNNNNNGG 2 cut(s) 304, 595
BseMI GCAATG 1 cut(s) 1099
BseMII CTCAG 5 cut(s) 572, 729, 744, 834, 1039
BseNI ACTGG 5 cut(s) 522, 582, 863, 937, 1092
BseRI GAGGAG 1 cut(s) 808
BseXI GCAGC 3 cut(s) 975, 1148, 1208
BsgI GTGCAG 1 cut(s) 704
BshFI GGCC 1 cut(s) 157
BshVI ATCGAT 1 cut(s) 427
BsiHKAI GWGCWC 1 cut(s) 517
BsiSI CCGG 2 cut(s) 66, 590
BslFI GGGAC 2 cut(s) 135, 186
BslI CCNNNNNNNGG 2 cut(s) 304, 595
BsmAI GTCTC 1 cut(s) 262
BsmFI GGGAC 2 cut(s) 135, 186
BsnI GGCC 1 cut(s) 157
Bsp119I TTCGAA 1 cut(s) 504
Bsp1286I GDGCHC 1 cut(s) 517
Bsp143I GATC 6 cut(s) 424, 481, 767, 881, 973, 1213
BspACI CCGC 1 cut(s) 909
BspANI GGCC 1 cut(s) 157
BspCNI CTCAG 5 cut(s) 571, 730, 745, 833, 1038
BspDI ATCGAT 1 cut(s) 427
BspLI GGNNCC 1 cut(s) 309
BspPI GGATC 3 cut(s) 432, 889, 1208
BspT104I TTCGAA 1 cut(s) 504
BspTI CTTAAG 1 cut(s) 790
BsrDI GCAATG 1 cut(s) 1099
BsrI ACTGG 5 cut(s) 522, 582, 863, 937, 1092
BssECI CCNNGG 3 cut(s) 118, 420, 799
BssMI GATC 6 cut(s) 424, 481, 767, 881, 973, 1213
BssT1I CCWWGG 1 cut(s) 799
Bst2UI CCWGG 3 cut(s) 119, 159, 422
Bst4CI ACNGT 3 cut(s) 275, 916, 959
Bst6I CTCTTC 2 cut(s) 125, 393
BstAFI CTTAAG 1 cut(s) 790
BstBI TTCGAA 1 cut(s) 504
BstC8I GCNNGC 2 cut(s) 285, 525
BstDEI CTNAG 5 cut(s) 558, 738, 753, 820, 1025
BstEII GGTNACC 1 cut(s) 656
BstF5I GGATG 4 cut(s) 37, 604, 611, 724
BstKTI GATC 6 cut(s) 427, 484, 770, 884, 976, 1216
BstMAI GTCTC 1 cut(s) 262
BstMBI GATC 6 cut(s) 424, 481, 767, 881, 973, 1213
BstMWI GCNNNNNNNGC 1 cut(s) 985
BstNI CCWGG 3 cut(s) 119, 159, 422
BstPI GGTNACC 1 cut(s) 656
BstSCI CCNGG 4 cut(s) 117, 157, 420, 588
BstV1I GCAGC 3 cut(s) 975, 1148, 1208
BstX2I RGATCY 1 cut(s) 1213
BstYI RGATCY 1 cut(s) 1213
Bsu15I ATCGAT 1 cut(s) 427
Bsu36I CCTNAGG 1 cut(s) 820
BsuRI GGCC 1 cut(s) 157
BsuTUI ATCGAT 1 cut(s) 427
BtsCI GGATG 4 cut(s) 37, 604, 611, 724
BtsIMutI CAGTG 3 cut(s) 271, 515, 964
Cac8I GCNNGC 2 cut(s) 285, 525
CaiI CAGNNNCTG 2 cut(s) 124, 1202
Cfr13I GGNCC 2 cut(s) 307, 691
ClaI ATCGAT 1 cut(s) 427
Csp6I GTAC 4 cut(s) 442, 745, 912, 1270
CviAII CATG 3 cut(s) 191, 715, 733
CviQI GTAC 4 cut(s) 442, 745, 912, 1270
DdeI CTNAG 5 cut(s) 558, 738, 753, 820, 1025
DpnI GATC 6 cut(s) 426, 483, 769, 883, 975, 1215
DpnII GATC 6 cut(s) 424, 481, 767, 881, 973, 1213
DrdI GACNNNNNNGTC 2 cut(s) 15, 654
DseDI GACNNNNNNGTC 2 cut(s) 15, 654
EaeI YGGCCR 1 cut(s) 155
Eam1104I CTCTTC 2 cut(s) 125, 393
EarI CTCTTC 2 cut(s) 125, 393
Eco130I CCWWGG 1 cut(s) 799
Eco32I GATATC 1 cut(s) 295
Eco47I GGWCC 2 cut(s) 307, 691
Eco57I CTGAAG 5 cut(s) 146, 417, 422, 452, 1086
Eco81I CCTNAGG 1 cut(s) 820
Eco91I GGTNACC 1 cut(s) 656
EcoO65I GGTNACC 1 cut(s) 656
EcoRI GAATTC 3 cut(s) 93, 415, 919
EcoRII CCWGG 3 cut(s) 117, 157, 420
EcoRV GATATC 1 cut(s) 295
EcoT14I CCWWGG 1 cut(s) 799
ErhI CCWWGG 1 cut(s) 799
FaeI CATG 3 cut(s) 194, 718, 736
FaqI GGGAC 2 cut(s) 135, 186
FatI CATG 3 cut(s) 190, 714, 732
FbaI TGATCA 1 cut(s) 973
FblI GTMKAC 1 cut(s) 646
Fnu4HI GCNGC 3 cut(s) 989, 1137, 1197
FokI GGATG 4 cut(s) 24, 598, 611, 731
Fsp4HI GCNGC 3 cut(s) 989, 1137, 1197
FspBI CTAG 3 cut(s) 551, 605, 632
GluI GCNGC 3 cut(s) 989, 1137, 1197
GsuI CTGGAG 2 cut(s) 539, 599
HaeIII GGCC 1 cut(s) 157
HapII CCGG 2 cut(s) 66, 590
Hin1II CATG 3 cut(s) 194, 718, 736
HindIII AAGCTT 1 cut(s) 937
HinfI GANTC 6 cut(s) 44, 145, 230, 628, 648, 869
HpaII CCGG 2 cut(s) 66, 590
HphI GGTGA 4 cut(s) 149, 650, 836, 852
Hpy166II GTNNAC 2 cut(s) 48, 647
Hpy188I TCNGA 9 cut(s) 299, 451, 490, 627, 772, 955, 1101, 1110, 1184
Hpy188III TCNNGA 6 cut(s) 41, 368, 551, 632, 1211, 1282
Hpy8I GTNNAC 2 cut(s) 48, 647
Hpy99I CGWCG 1 cut(s) 728
HpyAV CCTTC 5 cut(s) 336, 336, 476, 511, 682
HpyCH4III ACNGT 3 cut(s) 275, 916, 959
HpyCH4V TGCA 7 cut(s) 86, 459, 543, 685, 895, 1163, 1196
HpyF10VI GCNNNNNNNGC 1 cut(s) 985
HpyF3I CTNAG 5 cut(s) 558, 738, 753, 820, 1025
Hsp92II CATG 3 cut(s) 194, 718, 736
Ksp22I TGATCA 1 cut(s) 973
Kzo9I GATC 6 cut(s) 424, 481, 767, 881, 973, 1213
LmnI GCTCC 3 cut(s) 244, 520, 856
Lsp1109I GCAGC 3 cut(s) 975, 1148, 1208
LweI GCATC 2 cut(s) 544, 1172
MaeI CTAG 3 cut(s) 551, 605, 632
MaeIII GTNAC 3 cut(s) 656, 794, 1168
MalI GATC 6 cut(s) 426, 483, 769, 883, 975, 1215
MboI GATC 6 cut(s) 424, 481, 767, 881, 973, 1213
MboII GAAGA 9 cut(s) 139, 142, 321, 410, 428, 769, 974, 1079, 1212
MflI RGATCY 1 cut(s) 1213
MhlI GDGCHC 1 cut(s) 517
MlyI GAGTC 4 cut(s) 53, 239, 642, 878
MmeI TCCRAC 1 cut(s) 322
MnlI CCTC 8 cut(s) 61, 128, 163, 179, 394, 671, 829, 1210
MseI TTAA 7 cut(s) 23, 81, 618, 791, 941, 945, 1077
MslI CAYNNNNRTG 1 cut(s) 489
MspA1I CMGCKG 2 cut(s) 395, 979
MspCI CTTAAG 1 cut(s) 790
MspI CCGG 2 cut(s) 66, 590
MspR9I CCNGG 4 cut(s) 119, 159, 422, 590
MvaI CCWGG 3 cut(s) 119, 159, 422
MwoI GCNNNNNNNGC 1 cut(s) 985
NciI CCSGG 1 cut(s) 590
NdeII GATC 6 cut(s) 424, 481, 767, 881, 973, 1213
NlaIII CATG 3 cut(s) 194, 718, 736
NlaIV GGNNCC 1 cut(s) 309
NmuCI GTSAC 2 cut(s) 656, 794
NspV TTCGAA 1 cut(s) 504
PacI TTAATTAA 1 cut(s) 945
PfeI GAWTC 2 cut(s) 145, 628
PkrI GCNGC 3 cut(s) 990, 1138, 1198
PleI GAGTC 4 cut(s) 52, 238, 642, 877
PpsI GAGTC 4 cut(s) 52, 238, 642, 877
PshBI ATTAAT 1 cut(s) 945
Psp6I CCWGG 3 cut(s) 117, 157, 420
PspEI GGTNACC 1 cut(s) 656
PspGI CCWGG 3 cut(s) 117, 157, 420
PspN4I GGNNCC 1 cut(s) 309
PspPI GGNCC 2 cut(s) 307, 691
PstNI CAGNNNCTG 2 cut(s) 124, 1202
PsuI RGATCY 1 cut(s) 1213
PvuII CAGCTG 2 cut(s) 395, 979
RsaI GTAC 4 cut(s) 443, 746, 913, 1271
RsaNI GTAC 4 cut(s) 442, 745, 912, 1270
RseI CAYNNNNRTG 1 cut(s) 489
SaqAI TTAA 7 cut(s) 23, 81, 618, 791, 941, 945, 1077
SatI GCNGC 3 cut(s) 989, 1137, 1197
Sau3AI GATC 6 cut(s) 424, 481, 767, 881, 973, 1213
Sau96I GGNCC 2 cut(s) 307, 691
SchI GAGTC 4 cut(s) 53, 239, 642, 878
ScrFI CCNGG 4 cut(s) 119, 159, 422, 590
SduI GDGCHC 1 cut(s) 517
SfaNI GCATC 2 cut(s) 544, 1172
SfuI TTCGAA 1 cut(s) 504
SinI GGWCC 2 cut(s) 307, 691
SmiMI CAYNNNNRTG 1 cut(s) 489
SmlI CTYRAG 1 cut(s) 790
SmoI CTYRAG 1 cut(s) 790
SsiI CCGC 1 cut(s) 909
SspMI CTAG 3 cut(s) 551, 605, 632
StyD4I CCNGG 4 cut(s) 117, 157, 420, 588
StyI CCWWGG 1 cut(s) 799
TaaI ACNGT 3 cut(s) 275, 916, 959
TaqI TCGA 3 cut(s) 427, 504, 573
TatI WGTACW 2 cut(s) 441, 1269
TfiI GAWTC 2 cut(s) 145, 628
Tru1I TTAA 7 cut(s) 23, 81, 618, 791, 941, 945, 1077
Tru9I TTAA 7 cut(s) 23, 81, 618, 791, 941, 945, 1077
TscAI CASTG 3 cut(s) 278, 522, 964
TseFI GTSAC 2 cut(s) 656, 794
TseI GCWGC 3 cut(s) 988, 1136, 1196
Tsp45I GTSAC 2 cut(s) 656, 794
TspDTI ATGAA 8 cut(s) 307, 428, 615, 714, 843, 939, 1202, 1212
TspGWI ACGGA 1 cut(s) 683
TspRI CASTG 3 cut(s) 278, 522, 964
Vha464I CTTAAG 1 cut(s) 790
VpaK11BI GGWCC 2 cut(s) 307, 691
VspI ATTAAT 1 cut(s) 945
XapI RAATTY 7 cut(s) 93, 415, 506, 889, 919, 1115, 1189
XbaI TCTAGA 2 cut(s) 550, 631
XmiI GTMKAC 1 cut(s) 646
XspI CTAG 3 cut(s) 551, 605, 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.