AT2G04039

Protein of unknown function (DUF2996)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006674
Physical Location & Seq
Forward (+)
1333133 .. 1334566
1434 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G04039.2

Sequence Viewer

Length: 600 bp
ATGGCAACAATAGCAGGAGGATCATTTGGAGTCCCAAGCTCTCGTATCTCTATAACAACACCAACTCTCTCTTCCTCTTCTTTGCTTCCACCACTAACATTGCAATCAGGTACTAGAAAGGACAACCTTTTGCGGTGTGCCTTGCAAGAGTCTTCAACTTCTGCTGTTGCTACTGAAAAGAAAAACAAAGAAGAAGGAGAAGAGTCAACAGTGGCGGTTCCGGCAAAGAAACCTAAACCCGCAGCTAAAGCGGCTGCAGTGGCGAAACCGCTGAGACAAATGATGGAAGAAGACGTGATTCCTCCTTTACAAGCCATTCTTGAATCTCAAGATGATATCTCTGACATAGATTTATCTTTCCAAGACGATAAGTTGGAAGGTTTTTTCTTAAAGAAAAGTATTCCATATTCCTTTTGGGCCTTTTTCCCTACTGGAAACCTTACAGGAGCAAAAGGATTTTCAATTTCCTCACACGGGTCAGGTCCGAGCACCGTGGAACCATTTCTTGTCGACGAGAGGAAACCAACTGCGAACCACGTTGTGTTTTGGGTCGAGAAGCGTCTTGCTGCACAAGGGATCATCCCCGTTTGGAACCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2996

Protein Analysis

199

Amino Acids

21.42

Weight (kDa)

6.15

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2996 PF11210 54 - 157 7.1e-15 Protein of unknown function (DUF2996)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 510
AciI CCGC 5 cut(s) 133, 215, 240, 251, 269
AclWI GGATC 2 cut(s) 28, 584
AdeI CACNNNGTG 1 cut(s) 541
AfaI GTAC 1 cut(s) 112
AfiI CCNNNNNNNGG 1 cut(s) 474
AgsI TTSAA 3 cut(s) 156, 323, 462
AjiI CACGTC 1 cut(s) 295
AjuI GAANNNNNNNTTGG 2 cut(s) 55, 87
AluBI AGCT 2 cut(s) 39, 245
AluI AGCT 2 cut(s) 39, 245
Alw21I GWGCWC 1 cut(s) 491
Alw26I GTCTC 1 cut(s) 268
AlwI GGATC 2 cut(s) 28, 584
AoxI GGCC 1 cut(s) 417
ApeKI GCWGC 3 cut(s) 242, 254, 566
Asp700I GAANNNNTTC 1 cut(s) 501
AspS9I GGNCC 2 cut(s) 417, 482
AvaII GGWCC 1 cut(s) 482
BbsI GAAGAC 2 cut(s) 144, 297
Bbv12I GWGCWC 1 cut(s) 491
BbvI GCAGC 3 cut(s) 241, 254, 553
BccI CCATC 1 cut(s) 277
BcoDI GTCTC 1 cut(s) 268
BfaI CTAG 1 cut(s) 114
BfmI CTRYAG 1 cut(s) 255
BisI GCNGC 4 cut(s) 243, 252, 255, 567
BlsI GCNGC 4 cut(s) 244, 253, 256, 568
Bme18I GGWCC 1 cut(s) 482
BmgBI CACGTC 1 cut(s) 295
BmgT120I GGNCC 2 cut(s) 417, 482
BmiI GGNNCC 3 cut(s) 219, 498, 593
BpiI GAAGAC 2 cut(s) 144, 297
BpuEI CTTGAG 1 cut(s) 312
BsaJI CCNNGG 1 cut(s) 492
Bsc4I CCNNNNNNNGG 1 cut(s) 474
Bse1I ACTGG 1 cut(s) 436
Bse3DI GCAATG 1 cut(s) 98
BseDI CCNNGG 1 cut(s) 492
BseGI GGATG 1 cut(s) 579
BseLI CCNNNNNNNGG 1 cut(s) 474
BseMI GCAATG 1 cut(s) 98
BseMII CTCAG 1 cut(s) 263
BseNI ACTGG 1 cut(s) 436
BseXI GCAGC 3 cut(s) 241, 254, 553
BsgI GTGCAG 1 cut(s) 552
BshFI GGCC 1 cut(s) 419
BsiHKAI GWGCWC 1 cut(s) 491
BsiSI CCGG 1 cut(s) 221
BslFI GGGAC 1 cut(s) 17
BslI CCNNNNNNNGG 1 cut(s) 474
BsmAI GTCTC 1 cut(s) 268
BsmFI GGGAC 1 cut(s) 17
BsnI GGCC 1 cut(s) 419
Bsp1286I GDGCHC 1 cut(s) 491
Bsp143I GATC 2 cut(s) 20, 576
BspACI CCGC 5 cut(s) 133, 215, 240, 251, 269
BspANI GGCC 1 cut(s) 419
BspCNI CTCAG 1 cut(s) 264
BspLI GGNNCC 3 cut(s) 219, 498, 593
BspMAI CTGCAG 1 cut(s) 259
BspPI GGATC 2 cut(s) 28, 584
BsrDI GCAATG 1 cut(s) 98
BsrI ACTGG 1 cut(s) 436
BssECI CCNNGG 1 cut(s) 492
BssMI GATC 2 cut(s) 20, 576
Bst4CI ACNGT 2 cut(s) 211, 493
Bst6I CTCTTC 3 cut(s) 76, 82, 195
BstDEI CTNAG 1 cut(s) 272
BstDSI CCRYGG 1 cut(s) 492
BstF5I GGATG 1 cut(s) 579
BstKTI GATC 2 cut(s) 23, 579
BstMAI GTCTC 1 cut(s) 268
BstMBI GATC 2 cut(s) 20, 576
BstMWI GCNNNNNNNGC 5 cut(s) 11, 221, 248, 251, 260
BstSFI CTRYAG 1 cut(s) 255
BstV1I GCAGC 3 cut(s) 241, 254, 553
BstV2I GAAGAC 2 cut(s) 144, 297
BsuRI GGCC 1 cut(s) 419
BtgI CCRYGG 1 cut(s) 492
BtrI CACGTC 1 cut(s) 295
BtsCI GGATG 1 cut(s) 579
BtsI GCAGTG 1 cut(s) 264
BtsIMutI CAGTG 2 cut(s) 216, 264
Cfr13I GGNCC 2 cut(s) 417, 482
CseI GACGC 1 cut(s) 548
Csp6I GTAC 1 cut(s) 111
CspCI CAANNNNNGTGG 2 cut(s) 81, 116
CviJI RGCY 5 cut(s) 39, 245, 254, 314, 419
CviKI_1 RGCY 5 cut(s) 39, 245, 254, 314, 419
CviQI GTAC 1 cut(s) 111
DdeI CTNAG 1 cut(s) 272
DpnI GATC 2 cut(s) 22, 578
DpnII GATC 2 cut(s) 20, 576
DraIII CACNNNGTG 1 cut(s) 541
Eam1104I CTCTTC 3 cut(s) 76, 82, 195
EarI CTCTTC 3 cut(s) 76, 82, 195
Eco32I GATATC 1 cut(s) 337
Eco47I GGWCC 1 cut(s) 482
EcoRV GATATC 1 cut(s) 337
FaiI YATR 3 cut(s) 53, 347, 406
FalI AAGNNNNNCTT 2 cut(s) 303, 335
FaqI GGGAC 1 cut(s) 17
FauI CCCGC 1 cut(s) 247
FblI GTMKAC 1 cut(s) 510
Fnu4HI GCNGC 4 cut(s) 243, 252, 255, 567
FokI GGATG 1 cut(s) 566
Fsp4HI GCNGC 4 cut(s) 243, 252, 255, 567
FspBI CTAG 1 cut(s) 114
GluI GCNGC 4 cut(s) 243, 252, 255, 567
HaeIII GGCC 1 cut(s) 419
HapII CCGG 1 cut(s) 221
HgaI GACGC 1 cut(s) 548
HincII GTYRAC 2 cut(s) 207, 511
HindII GTYRAC 2 cut(s) 207, 511
HinfI GANTC 5 cut(s) 30, 149, 203, 298, 323
HpaII CCGG 1 cut(s) 221
Hpy166II GTNNAC 2 cut(s) 207, 511
Hpy188I TCNGA 2 cut(s) 343, 486
Hpy188III TCNNGA 3 cut(s) 320, 329, 553
Hpy8I GTNNAC 2 cut(s) 207, 511
Hpy99I CGWCG 1 cut(s) 515
HpyAV CCTTC 2 cut(s) 188, 371
HpyCH4III ACNGT 2 cut(s) 211, 493
HpyCH4IV ACGT 2 cut(s) 294, 537
HpyCH4V TGCA 4 cut(s) 103, 145, 257, 569
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 221, 248, 251, 260
HpyF3I CTNAG 1 cut(s) 272
HpySE526I ACGT 2 cut(s) 294, 537
Kzo9I GATC 2 cut(s) 20, 576
LmnI GCTCC 1 cut(s) 446
LpnPI CCDG 5 cut(s) 93, 234, 417, 429, 465
Lsp1109I GCAGC 3 cut(s) 241, 254, 553
MaeI CTAG 1 cut(s) 114
MaeII ACGT 2 cut(s) 294, 537
MalI GATC 2 cut(s) 22, 578
MboI GATC 2 cut(s) 20, 576
MboII GAAGA 7 cut(s) 63, 69, 144, 203, 212, 299, 302
MhlI GDGCHC 1 cut(s) 491
MluCI AATT 1 cut(s) 462
MlyI GAGTC 3 cut(s) 39, 158, 212
MmeI TCCRAC 1 cut(s) 354
MnlI CCTC 5 cut(s) 11, 85, 312, 478, 510
MroXI GAANNNNTTC 1 cut(s) 501
MseI TTAA 1 cut(s) 389
MspA1I CMGCKG 1 cut(s) 271
MspI CCGG 1 cut(s) 221
MwoI GCNNNNNNNGC 5 cut(s) 11, 221, 248, 251, 260
NdeII GATC 2 cut(s) 20, 576
NlaIV GGNNCC 3 cut(s) 219, 498, 593
PdmI GAANNNNTTC 1 cut(s) 501
PfeI GAWTC 2 cut(s) 298, 323
PkrI GCNGC 4 cut(s) 244, 253, 256, 568
PleI GAGTC 3 cut(s) 38, 157, 211
PpsI GAGTC 3 cut(s) 38, 157, 211
PspN4I GGNNCC 3 cut(s) 219, 498, 593
PspPI GGNCC 2 cut(s) 417, 482
PstI CTGCAG 1 cut(s) 259
RsaI GTAC 1 cut(s) 112
RsaNI GTAC 1 cut(s) 111
SalI GTCGAC 1 cut(s) 509
SaqAI TTAA 1 cut(s) 389
SatI GCNGC 4 cut(s) 243, 252, 255, 567
Sau3AI GATC 2 cut(s) 20, 576
Sau96I GGNCC 2 cut(s) 417, 482
SchI GAGTC 3 cut(s) 39, 158, 212
SduI GDGCHC 1 cut(s) 491
SfcI CTRYAG 1 cut(s) 255
SinI GGWCC 1 cut(s) 482
SmlI CTYRAG 1 cut(s) 327
SmoI CTYRAG 1 cut(s) 327
Sse9I AATT 1 cut(s) 462
SsiI CCGC 5 cut(s) 133, 215, 240, 251, 269
SspMI CTAG 1 cut(s) 114
TaaI ACNGT 2 cut(s) 211, 493
TaiI ACGT 2 cut(s) 297, 540
TaqI TCGA 2 cut(s) 510, 552
TasI AATT 1 cut(s) 462
TauI GCSGC 1 cut(s) 254
TfiI GAWTC 2 cut(s) 298, 323
Tru1I TTAA 1 cut(s) 389
Tru9I TTAA 1 cut(s) 389
TscAI CASTG 2 cut(s) 216, 264
TseI GCWGC 3 cut(s) 242, 254, 566
TspRI CASTG 2 cut(s) 216, 264
VpaK11BI GGWCC 1 cut(s) 482
XcmI CCANNNNNNNNNTGG 1 cut(s) 411
XmiI GTMKAC 1 cut(s) 510
XmnI GAANNNNTTC 1 cut(s) 501
XspI CTAG 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.