FvH4_5g27060

Protein of unknown function (DUF2996)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006674
Physical Location & Seq
Reverse (-)
18363827 .. 18365511
1685 bp
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UTR
Exon/CDS
Intron
FvH4_5g27060.t2

Sequence Viewer

Length: 654 bp
ATGGCTTCGGCTAATATTCTTTCTATGGCCAAACCATCTCCTCAGGAAAATGGGAAGGGGTTTGTAGAATTCTCAAGTACTCTGCGCAACACATCAGCTAATTGCCTTCAGTTTGGAAGGAAGAACTCCGATGATTCTGTCTCTGTTGTTGCCTTCCATAGTTCCCCTGCTCAAATGGTAAGTAAAGCGGTAGCATCTAAAAGAACTATCGCCTGCTCTGCTCTGCAAGAATCATCTACTTCCACTGTTGCTGCTGAAACGAAAGAAGTGAAAGCAGCTCCAGCAAAGCCTAAAGCTCCGGCAAAAGCTCCAGTCAAGCCGTTACCTCAAATGATGGAAGAGGATATAATCCCATCGCTGAAAGCAATACTCGAAACTCCTCAAAATCAGCTCTCTGATATTGAGCTATTTTTTGAAGACAACAAGCTTGAAGGTTCGTTTCTGAAGAAGGGCAATTCATATTCATTTTGGGCATTCTTTCCCAACGGAGACCTCACAGGTCCAAAAGGCTTTTCTTTATCTTCCTACGGCTTAGGAGCAAGCACTGTCGAACCTTTTCTCGTTGATGAGAAGAAGATTACGGCAAGACACATTATCTTTTGGGTTGAAAAGCGTTTGGCAGCTCAAGGAATCATTCCTGTGTGGAAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2996

Protein Analysis

218

Amino Acids

23.49

Weight (kDa)

8.78

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2996 PF11210 107 - 210 1.6e-13 Protein of unknown function (DUF2996)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 86
AciI CCGC 1 cut(s) 188
AcoI YGGCCR 1 cut(s) 27
AcsI RAATTY 1 cut(s) 68
AcuI CTGAAG 2 cut(s) 92, 464
AfaI GTAC 1 cut(s) 79
AgsI TTSAA 3 cut(s) 416, 431, 608
AjuI GAANNNNNNNTTGG 2 cut(s) 496, 528
AluBI AGCT 8 cut(s) 98, 278, 296, 308, 391, 406, 427, 623
AluI AGCT 8 cut(s) 98, 278, 296, 308, 391, 406, 427, 623
Alw26I GTCTC 2 cut(s) 145, 483
AoxI GGCC 1 cut(s) 27
ApeKI GCWGC 3 cut(s) 251, 275, 620
ApoI RAATTY 1 cut(s) 68
Asp700I GAANNNNTTC 1 cut(s) 555
AspLEI GCGC 1 cut(s) 87
AspS9I GGNCC 1 cut(s) 500
AvaII GGWCC 1 cut(s) 500
AxyI CCTNAGG 1 cut(s) 42
BalI TGGCCA 1 cut(s) 29
BbsI GAAGAC 1 cut(s) 423
BbvI GCAGC 3 cut(s) 238, 287, 632
BccI CCATC 3 cut(s) 43, 328, 361
BceAI ACGGC 3 cut(s) 304, 544, 597
BcoDI GTCTC 2 cut(s) 145, 483
BisI GCNGC 3 cut(s) 252, 276, 621
BlsI GCNGC 3 cut(s) 253, 277, 622
BmcAI AGTACT 1 cut(s) 79
Bme18I GGWCC 1 cut(s) 500
BmgT120I GGNCC 1 cut(s) 500
BmsI GCATC 1 cut(s) 203
BpiI GAAGAC 1 cut(s) 423
BpmI CTGGAG 2 cut(s) 264, 294
Bpu10I CCTNAGC 1 cut(s) 532
BpuEI CTTGAG 2 cut(s) 58, 609
BsaI GGTCTC 1 cut(s) 483
Bse1I ACTGG 1 cut(s) 311
Bse21I CCTNAGG 1 cut(s) 42
BseMII CTCAG 1 cut(s) 56
BseNI ACTGG 1 cut(s) 311
BseRI GAGGAG 2 cut(s) 30, 369
BseXI GCAGC 3 cut(s) 238, 287, 632
BshFI GGCC 1 cut(s) 29
BsiSI CCGG 1 cut(s) 299
BsmAI GTCTC 2 cut(s) 145, 483
BsmI GAATGC 1 cut(s) 473
BsnI GGCC 1 cut(s) 29
Bso31I GGTCTC 1 cut(s) 483
BspACI CCGC 1 cut(s) 188
BspANI GGCC 1 cut(s) 29
BspCNI CTCAG 1 cut(s) 55
BspTNI GGTCTC 1 cut(s) 483
BsrI ACTGG 1 cut(s) 311
Bst4CI ACNGT 2 cut(s) 247, 547
Bst6I CTCTTC 1 cut(s) 333
BstC8I GCNNGC 2 cut(s) 214, 541
BstDEI CTNAG 2 cut(s) 42, 532
BstHHI GCGC 1 cut(s) 87
BstMAI GTCTC 2 cut(s) 145, 483
BstMWI GCNNNNNNNGC 2 cut(s) 218, 281
BstV1I GCAGC 3 cut(s) 238, 287, 632
BstV2I GAAGAC 1 cut(s) 423
Bsu36I CCTNAGG 1 cut(s) 42
BsuRI GGCC 1 cut(s) 29
BtgZI GCGATG 1 cut(s) 339
BtsIMutI CAGTG 2 cut(s) 243, 543
Cac8I GCNNGC 2 cut(s) 214, 541
CfoI GCGC 1 cut(s) 87
Cfr13I GGNCC 1 cut(s) 500
Csp6I GTAC 1 cut(s) 78
CviQI GTAC 1 cut(s) 78
DdeI CTNAG 2 cut(s) 42, 532
EaeI YGGCCR 1 cut(s) 27
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
Eco31I GGTCTC 1 cut(s) 483
Eco47I GGWCC 1 cut(s) 500
Eco57I CTGAAG 2 cut(s) 92, 464
Eco81I CCTNAGG 1 cut(s) 42
EcoRI GAATTC 1 cut(s) 68
FaiI YATR 4 cut(s) 26, 159, 347, 460
Fnu4HI GCNGC 3 cut(s) 252, 276, 621
Fsp4HI GCNGC 3 cut(s) 252, 276, 621
FspI TGCGCA 1 cut(s) 86
GlaI GCGC 1 cut(s) 86
GluI GCNGC 3 cut(s) 252, 276, 621
GsuI CTGGAG 2 cut(s) 264, 294
HaeIII GGCC 1 cut(s) 29
HapII CCGG 1 cut(s) 299
HhaI GCGC 1 cut(s) 87
Hin6I GCGC 1 cut(s) 85
HinP1I GCGC 1 cut(s) 85
HindIII AAGCTT 1 cut(s) 425
HinfI GANTC 3 cut(s) 134, 230, 630
HpaII CCGG 1 cut(s) 299
Hpy188I TCNGA 3 cut(s) 130, 397, 444
Hpy188III TCNNGA 1 cut(s) 44
HpyAV CCTTC 6 cut(s) 49, 111, 116, 163, 425, 442
HpyCH4III ACNGT 2 cut(s) 247, 547
HpyCH4V TGCA 1 cut(s) 226
HpyF10VI GCNNNNNNNGC 2 cut(s) 218, 281
HpyF3I CTNAG 2 cut(s) 42, 532
HspAI GCGC 1 cut(s) 85
LmnI GCTCC 4 cut(s) 283, 301, 313, 536
LpnPI CCDG 7 cut(s) 29, 180, 226, 294, 312, 324, 483
Lsp1109I GCAGC 3 cut(s) 238, 287, 632
LweI GCATC 1 cut(s) 203
MaeIII GTNAC 1 cut(s) 321
MboII GAAGA 7 cut(s) 133, 350, 428, 457, 513, 583, 586
MlsI TGGCCA 1 cut(s) 29
MluCI AATT 3 cut(s) 68, 100, 454
MluNI TGGCCA 1 cut(s) 29
MnlI CCTC 5 cut(s) 51, 334, 336, 390, 503
Mox20I TGGCCA 1 cut(s) 29
MroXI GAANNNNTTC 1 cut(s) 555
MscI TGGCCA 1 cut(s) 29
MslI CAYNNNNRTG 1 cut(s) 638
Msp20I TGGCCA 1 cut(s) 29
MspI CCGG 1 cut(s) 299
Mva1269I GAATGC 1 cut(s) 473
MwoI GCNNNNNNNGC 2 cut(s) 218, 281
NsbI TGCGCA 1 cut(s) 86
PctI GAATGC 1 cut(s) 473
PdmI GAANNNNTTC 1 cut(s) 555
PfeI GAWTC 3 cut(s) 134, 230, 630
PkrI GCNGC 3 cut(s) 253, 277, 622
PspPI GGNCC 1 cut(s) 500
RsaI GTAC 1 cut(s) 79
RsaNI GTAC 1 cut(s) 78
RseI CAYNNNNRTG 1 cut(s) 638
SatI GCNGC 3 cut(s) 252, 276, 621
Sau96I GGNCC 1 cut(s) 500
ScaI AGTACT 1 cut(s) 79
SfaNI GCATC 1 cut(s) 203
SinI GGWCC 1 cut(s) 500
SmiMI CAYNNNNRTG 1 cut(s) 638
SmlI CTYRAG 2 cut(s) 73, 624
SmoI CTYRAG 2 cut(s) 73, 624
Sse9I AATT 3 cut(s) 68, 100, 454
SsiI CCGC 1 cut(s) 188
SspI AATATT 1 cut(s) 16
TaaI ACNGT 2 cut(s) 247, 547
TaqI TCGA 2 cut(s) 372, 549
TasI AATT 3 cut(s) 68, 100, 454
TatI WGTACW 1 cut(s) 77
TfiI GAWTC 3 cut(s) 134, 230, 630
TscAI CASTG 2 cut(s) 250, 550
TseI GCWGC 3 cut(s) 251, 275, 620
TspDTI ATGAA 2 cut(s) 447, 453
TspGWI ACGGA 1 cut(s) 501
TspRI CASTG 2 cut(s) 250, 550
VpaK11BI GGWCC 1 cut(s) 500
XapI RAATTY 1 cut(s) 68
XmnI GAANNNNTTC 1 cut(s) 555
ZrmI AGTACT 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.