MD00G1144800.v1.1

Protein of unknown function (DUF2996)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0006674
Physical Location & Seq
Forward (+)
31706088 .. 31707768
1681 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1144800.v1.1.491

Sequence Viewer

Length: 675 bp
ATGAAGCTCCAAATTAACAACCAAGCAAGCATGGCTTCGGCTATTCTTTCTCTGGCCAAACCATCTCCTCTGCAGCCAAATGGAAAACATTTTGCAGAATTCTCAGCTCTCCCGAAGCCATCAGCCTGCCTGCCTTTTGGCAGAAAGACCTCAAACGATCAAATGATGAAAAGTAAAGTGGTAGCATCTCAAAGAAATATTGCGTGCTTTGCTTTGCAAGAATCATCCACTTCCACTGCTGATGCTGCTGCTGCTGCTGATACAAGTACAAAGGAGGTTAAAACAGCTGATCAAAAAGCAGCAGCTCCAGCAAAGCCTAAAGTTGCTGCAAAAGCCCCAATGAAGGAGTTGCCTCAAATGATGGAGGAGGACGTTATCCCGCCGTTGAAAGCAATACTCGAAACTCAAGATGAACTCTCTGATATTGAGCTATGTTTTCAAGACAACAGGCTGGAAGGTTCCTTTATAAAGAAGGGCAATTCTTATTCGTTTTGGGCCTTCTTCCCCAGTGGAGTCCTCACAGGTCCAAAGGGGTTTTCTCTGTCTTCGTATGGCTCAGGAGTGAGCACTGTTGAGCCATTTCTCGTTGATGAGAAGAAAATAACTGCAAAACATATTGTCTTTTGGGTTGAAAAGCGTTTAGCCGCTCAAGGAATCATTCCTGTCTGGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2996

Protein Analysis

225

Amino Acids

24.21

Weight (kDa)

8.88

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2996 PF11210 118 - 217 5.2e-12 Protein of unknown function (DUF2996)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 467
AccBSI CCGCTC 1 cut(s) 647
AciI CCGC 2 cut(s) 380, 645
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 1 cut(s) 98
AfaI GTAC 1 cut(s) 268
AfiI CCNNNNNNNGG 1 cut(s) 343
AgsI TTSAA 3 cut(s) 388, 440, 632
AjuI GAANNNNNNNTTGG 2 cut(s) 520, 552
AluBI AGCT 5 cut(s) 7, 107, 287, 305, 430
AluI AGCT 5 cut(s) 7, 107, 287, 305, 430
Alw21I GWGCWC 1 cut(s) 569
AoxI GGCC 2 cut(s) 54, 495
ApeKI GCWGC 8 cut(s) 73, 245, 248, 251, 254, 299, 302, 326
ApoI RAATTY 1 cut(s) 98
AspS9I GGNCC 2 cut(s) 495, 524
AvaII GGWCC 1 cut(s) 524
BalI TGGCCA 1 cut(s) 56
BbsI GAAGAC 1 cut(s) 537
Bbv12I GWGCWC 1 cut(s) 569
BbvI GCAGC 8 cut(s) 85, 232, 235, 238, 241, 311, 313, 314
BccI CCATC 3 cut(s) 70, 127, 355
BceAI ACGGC 1 cut(s) 367
BclI TGATCA 1 cut(s) 289
BfmI CTRYAG 1 cut(s) 71
BisI GCNGC 9 cut(s) 74, 246, 249, 252, 255, 300, 303, 327, 645
BlsI GCNGC 9 cut(s) 75, 247, 250, 253, 256, 301, 304, 328, 646
Bme18I GGWCC 1 cut(s) 524
BmgT120I GGNCC 2 cut(s) 495, 524
BmiI GGNNCC 1 cut(s) 460
BmrI ACTGGG 1 cut(s) 501
BmsI GCATC 2 cut(s) 194, 232
BmuI ACTGGG 1 cut(s) 501
BpiI GAAGAC 1 cut(s) 537
BpmI CTGGAG 1 cut(s) 291
Bpu10I CCTNAGC 1 cut(s) 556
BpuEI CTTGAG 2 cut(s) 390, 633
Bsc4I CCNNNNNNNGG 1 cut(s) 343
Bse1I ACTGG 1 cut(s) 507
BseGI GGATG 1 cut(s) 224
BseLI CCNNNNNNNGG 1 cut(s) 343
BseMII CTCAG 2 cut(s) 117, 570
BseNI ACTGG 1 cut(s) 507
BseRI GAGGAG 2 cut(s) 57, 380
BseXI GCAGC 8 cut(s) 85, 232, 235, 238, 241, 311, 313, 314
BshFI GGCC 2 cut(s) 56, 497
BsiHKAI GWGCWC 1 cut(s) 569
BslI CCNNNNNNNGG 1 cut(s) 343
BsnI GGCC 2 cut(s) 56, 497
Bsp1286I GDGCHC 1 cut(s) 569
Bsp143I GATC 2 cut(s) 157, 289
BspACI CCGC 2 cut(s) 380, 645
BspANI GGCC 2 cut(s) 56, 497
BspCNI CTCAG 2 cut(s) 116, 569
BspLI GGNNCC 1 cut(s) 460
BspMAI CTGCAG 1 cut(s) 75
BsrBI CCGCTC 1 cut(s) 647
BsrI ACTGG 1 cut(s) 507
BssMI GATC 2 cut(s) 157, 289
Bst4CI ACNGT 1 cut(s) 571
BstC8I GCNNGC 4 cut(s) 28, 127, 131, 205
BstDEI CTNAG 2 cut(s) 103, 556
BstF5I GGATG 1 cut(s) 224
BstKTI GATC 2 cut(s) 160, 292
BstMBI GATC 2 cut(s) 157, 289
BstMWI GCNNNNNNNGC 7 cut(s) 32, 209, 245, 251, 254, 308, 332
BstSFI CTRYAG 1 cut(s) 71
BstV1I GCAGC 8 cut(s) 85, 232, 235, 238, 241, 311, 313, 314
BstV2I GAAGAC 1 cut(s) 537
BsuRI GGCC 2 cut(s) 56, 497
BtsCI GGATG 1 cut(s) 224
BtsI GCAGTG 1 cut(s) 234
BtsIMutI CAGTG 3 cut(s) 234, 514, 567
Cac8I GCNNGC 4 cut(s) 28, 127, 131, 205
Cfr13I GGNCC 2 cut(s) 495, 524
Csp6I GTAC 1 cut(s) 267
CviAII CATG 1 cut(s) 31
CviQI GTAC 1 cut(s) 267
DdeI CTNAG 2 cut(s) 103, 556
DpnI GATC 2 cut(s) 159, 291
DpnII GATC 2 cut(s) 157, 289
EaeI YGGCCR 1 cut(s) 54
Eco47I GGWCC 1 cut(s) 524
EcoRI GAATTC 1 cut(s) 98
FaeI CATG 1 cut(s) 34
FaiI YATR 5 cut(s) 32, 433, 467, 552, 615
FalI AAGNNNNNCTT 2 cut(s) 19, 51
FatI CATG 1 cut(s) 30
FauI CCCGC 1 cut(s) 387
FbaI TGATCA 1 cut(s) 289
Fnu4HI GCNGC 9 cut(s) 74, 246, 249, 252, 255, 300, 303, 327, 645
FokI GGATG 1 cut(s) 211
Fsp4HI GCNGC 9 cut(s) 74, 246, 249, 252, 255, 300, 303, 327, 645
GluI GCNGC 9 cut(s) 74, 246, 249, 252, 255, 300, 303, 327, 645
GsuI CTGGAG 1 cut(s) 291
HaeIII GGCC 2 cut(s) 56, 497
Hin1II CATG 1 cut(s) 34
HinfI GANTC 3 cut(s) 221, 513, 654
Hpy188I TCNGA 1 cut(s) 421
Hpy188III TCNNGA 5 cut(s) 112, 407, 440, 558, 667
HpyAV CCTTC 4 cut(s) 337, 449, 466, 508
HpyCH4III ACNGT 1 cut(s) 571
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 5 cut(s) 73, 95, 217, 329, 608
HpyF10VI GCNNNNNNNGC 7 cut(s) 32, 209, 245, 251, 254, 308, 332
HpyF3I CTNAG 2 cut(s) 103, 556
HpySE526I ACGT 1 cut(s) 372
Hsp92II CATG 1 cut(s) 34
Ksp22I TGATCA 1 cut(s) 289
Kzo9I GATC 2 cut(s) 157, 289
LmnI GCTCC 2 cut(s) 12, 310
Lsp1109I GCAGC 8 cut(s) 85, 232, 235, 238, 241, 311, 313, 314
LweI GCATC 2 cut(s) 194, 232
MaeII ACGT 1 cut(s) 372
MalI GATC 2 cut(s) 159, 291
MbiI CCGCTC 1 cut(s) 647
MboI GATC 2 cut(s) 157, 289
MboII GAAGA 3 cut(s) 493, 537, 607
MhlI GDGCHC 1 cut(s) 569
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 4 cut(s) 12, 98, 478, 670
MluNI TGGCCA 1 cut(s) 56
MlyI GAGTC 1 cut(s) 522
MnlI CCTC 7 cut(s) 78, 160, 268, 358, 361, 363, 527
Mox20I TGGCCA 1 cut(s) 56
MscI TGGCCA 1 cut(s) 56
MseI TTAA 2 cut(s) 15, 279
Msp20I TGGCCA 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 287
MwoI GCNNNNNNNGC 7 cut(s) 32, 209, 245, 251, 254, 308, 332
NdeII GATC 2 cut(s) 157, 289
NlaIII CATG 1 cut(s) 34
NlaIV GGNNCC 1 cut(s) 460
PfeI GAWTC 2 cut(s) 221, 654
PkrI GCNGC 9 cut(s) 75, 247, 250, 253, 256, 301, 304, 328, 646
PleI GAGTC 1 cut(s) 521
PpsI GAGTC 1 cut(s) 521
PsiI TTATAA 1 cut(s) 467
PspN4I GGNNCC 1 cut(s) 460
PspPI GGNCC 2 cut(s) 495, 524
PstI CTGCAG 1 cut(s) 75
PvuII CAGCTG 1 cut(s) 287
RsaI GTAC 1 cut(s) 268
RsaNI GTAC 1 cut(s) 267
SaqAI TTAA 2 cut(s) 15, 279
SatI GCNGC 9 cut(s) 74, 246, 249, 252, 255, 300, 303, 327, 645
Sau3AI GATC 2 cut(s) 157, 289
Sau96I GGNCC 2 cut(s) 495, 524
SchI GAGTC 1 cut(s) 522
SduI GDGCHC 1 cut(s) 569
SfaNI GCATC 2 cut(s) 194, 232
SfcI CTRYAG 1 cut(s) 71
SinI GGWCC 1 cut(s) 524
SmlI CTYRAG 2 cut(s) 405, 648
SmoI CTYRAG 2 cut(s) 405, 648
Sse9I AATT 4 cut(s) 12, 98, 478, 670
SsiI CCGC 2 cut(s) 380, 645
SspI AATATT 1 cut(s) 199
TaaI ACNGT 1 cut(s) 571
TaiI ACGT 1 cut(s) 375
TaqI TCGA 1 cut(s) 399
TasI AATT 4 cut(s) 12, 98, 478, 670
TatI WGTACW 1 cut(s) 266
TauI GCSGC 1 cut(s) 647
TfiI GAWTC 2 cut(s) 221, 654
Tru1I TTAA 2 cut(s) 15, 279
Tru9I TTAA 2 cut(s) 15, 279
TscAI CASTG 3 cut(s) 241, 514, 574
TseI GCWGC 8 cut(s) 73, 245, 248, 251, 254, 299, 302, 326
TspDTI ATGAA 4 cut(s) 17, 182, 356, 426
TspRI CASTG 3 cut(s) 241, 514, 574
VpaK11BI GGWCC 1 cut(s) 524
XapI RAATTY 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.