Rh7BG305900

Protein of unknown function (DUF2996)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0006674
Physical Location & Seq
Reverse (-)
29176742 .. 29177985
1244 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG305900.1

Sequence Viewer

Length: 651 bp
ATGGCTTCGGCTAATATTCTTTCTATGGCCAAACCATCTCCTCAGGAAAATGGGAAGGGGTTTGTAGAATTCTCAGGTACTCTGCGGAACTCATCAGCTAATTGCCTTCAGTTTGGAAGGAAGAACTCGGACGACTTTGCCTTCCATAGTTCCTCTGCTCATAAGGTAAGTAAACCAGTAGCACCTAAAAGAATTATTGCCTGCTCTGCTCTGCAAGAATCATCTACTTCATCTACTTCCACTGTTGCCGCTGAAACAAAAGAGGTGAAAGCAGCTCCAGCAAAGCCTAAAGCTCCGGCAAAAGCTCCAGTCAAGCCGCTACCTCAAATGATGGAAGAGGATATTATCCCATCGCTGAAAGCAATACTCGAAACTCCTCAAAATGAGCTCTCTGACCTAGAACTATTCTTTGAAGACAACAAGCTTGAAGGTTCGTTTCTGAAGAAGGGCAATTCGTACTCATTTTGGGCGTTCTTTCCCAATGGAGTCCTCACAGGTCCAAAAGGGTTTTCTTTATCTTCCTGCGGCTTGGGCGCAAGCACTGTTGAACCTTTTCTTGTTGACGAGAAGAAGATCACGGCAAGACACATTATCTTTTGGGTTGAAAAACGTTTGGCAGCTCAAGGAATCATTCCTGTGTGGAAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2996

Protein Analysis

216

Amino Acids

23.36

Weight (kDa)

8.91

Isoelectric Point (pI)

49.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2996 PF11210 105 - 208 1.3e-12 Protein of unknown function (DUF2996)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 85, 249, 317, 525
AclI AACGTT 1 cut(s) 610
AcoI YGGCCR 1 cut(s) 27
AcsI RAATTY 1 cut(s) 68
AcuI CTGAAG 2 cut(s) 92, 461
AfaI GTAC 2 cut(s) 79, 458
AgsI TTSAA 4 cut(s) 413, 428, 548, 605
AjuI GAANNNNNNNTTGG 2 cut(s) 493, 525
AluBI AGCT 7 cut(s) 98, 275, 293, 305, 388, 424, 620
AluI AGCT 7 cut(s) 98, 275, 293, 305, 388, 424, 620
Alw21I GWGCWC 1 cut(s) 390
AoxI GGCC 1 cut(s) 27
ApeKI GCWGC 2 cut(s) 272, 617
ApoI RAATTY 1 cut(s) 68
Asp700I GAANNNNTTC 1 cut(s) 552
AspLEI GCGC 1 cut(s) 536
AspS9I GGNCC 1 cut(s) 497
AsuHPI GGTGA 1 cut(s) 277
AvaII GGWCC 1 cut(s) 497
AxyI CCTNAGG 1 cut(s) 42
BalI TGGCCA 1 cut(s) 29
BanII GRGCYC 1 cut(s) 390
BbsI GAAGAC 1 cut(s) 420
Bbv12I GWGCWC 1 cut(s) 390
BbvI GCAGC 2 cut(s) 284, 629
BccI CCATC 3 cut(s) 43, 325, 358
BceAI ACGGC 1 cut(s) 594
BfaI CTAG 1 cut(s) 398
BisI GCNGC 5 cut(s) 249, 273, 317, 526, 618
BlsI GCNGC 5 cut(s) 250, 274, 318, 527, 619
Bme18I GGWCC 1 cut(s) 497
BmgT120I GGNCC 1 cut(s) 497
BpiI GAAGAC 1 cut(s) 420
BpmI CTGGAG 2 cut(s) 261, 291
BpuEI CTTGAG 1 cut(s) 606
Bse1I ACTGG 2 cut(s) 176, 308
Bse21I CCTNAGG 1 cut(s) 42
BseMII CTCAG 2 cut(s) 56, 87
BseNI ACTGG 2 cut(s) 176, 308
BseRI GAGGAG 2 cut(s) 30, 366
BseXI GCAGC 2 cut(s) 284, 629
BshFI GGCC 1 cut(s) 29
BsiHKAI GWGCWC 1 cut(s) 390
BsiSI CCGG 1 cut(s) 296
BsnI GGCC 1 cut(s) 29
Bsp1286I GDGCHC 1 cut(s) 390
Bsp143I GATC 1 cut(s) 573
BspACI CCGC 4 cut(s) 85, 249, 317, 525
BspANI GGCC 1 cut(s) 29
BspCNI CTCAG 2 cut(s) 55, 86
BsrI ACTGG 2 cut(s) 176, 308
BssMI GATC 1 cut(s) 573
Bst4CI ACNGT 2 cut(s) 244, 544
Bst6I CTCTTC 1 cut(s) 330
BstC8I GCNNGC 2 cut(s) 202, 538
BstDEI CTNAG 2 cut(s) 42, 73
BstHHI GCGC 1 cut(s) 536
BstKTI GATC 1 cut(s) 576
BstMBI GATC 1 cut(s) 573
BstMWI GCNNNNNNNGC 3 cut(s) 206, 278, 531
BstV1I GCAGC 2 cut(s) 284, 629
BstV2I GAAGAC 1 cut(s) 420
Bsu36I CCTNAGG 1 cut(s) 42
BsuRI GGCC 1 cut(s) 29
BtgZI GCGATG 1 cut(s) 336
BtsIMutI CAGTG 2 cut(s) 240, 540
Cac8I GCNNGC 2 cut(s) 202, 538
CfoI GCGC 1 cut(s) 536
Cfr13I GGNCC 1 cut(s) 497
Csp6I GTAC 2 cut(s) 78, 457
CviQI GTAC 2 cut(s) 78, 457
DdeI CTNAG 2 cut(s) 42, 73
DpnI GATC 1 cut(s) 575
DpnII GATC 1 cut(s) 573
EaeI YGGCCR 1 cut(s) 27
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
Ecl136II GAGCTC 1 cut(s) 388
Eco24I GRGCYC 1 cut(s) 390
Eco47I GGWCC 1 cut(s) 497
Eco53kI GAGCTC 1 cut(s) 388
Eco57I CTGAAG 2 cut(s) 92, 461
Eco81I CCTNAGG 1 cut(s) 42
EcoICRI GAGCTC 1 cut(s) 388
EcoRI GAATTC 1 cut(s) 68
EcoT38I GRGCYC 1 cut(s) 390
FaiI YATR 3 cut(s) 26, 147, 162
Fnu4HI GCNGC 5 cut(s) 249, 273, 317, 526, 618
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 5 cut(s) 249, 273, 317, 526, 618
FspBI CTAG 1 cut(s) 398
GlaI GCGC 1 cut(s) 535
GluI GCNGC 5 cut(s) 249, 273, 317, 526, 618
GsuI CTGGAG 2 cut(s) 261, 291
HaeIII GGCC 1 cut(s) 29
HapII CCGG 1 cut(s) 296
HhaI GCGC 1 cut(s) 536
Hin6I GCGC 1 cut(s) 534
HinP1I GCGC 1 cut(s) 534
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HindIII AAGCTT 1 cut(s) 422
HinfI GANTC 3 cut(s) 218, 486, 627
HpaII CCGG 1 cut(s) 296
HphI GGTGA 1 cut(s) 277
Hpy166II GTNNAC 2 cut(s) 173, 562
Hpy188I TCNGA 3 cut(s) 130, 394, 441
Hpy188III TCNNGA 1 cut(s) 44
Hpy8I GTNNAC 2 cut(s) 173, 562
HpyAV CCTTC 6 cut(s) 49, 111, 116, 151, 422, 439
HpyCH4III ACNGT 2 cut(s) 244, 544
HpyCH4IV ACGT 1 cut(s) 610
HpyCH4V TGCA 1 cut(s) 214
HpyF10VI GCNNNNNNNGC 3 cut(s) 206, 278, 531
HpyF3I CTNAG 2 cut(s) 42, 73
HpySE526I ACGT 1 cut(s) 610
HspAI GCGC 1 cut(s) 534
Kzo9I GATC 1 cut(s) 573
LmnI GCTCC 3 cut(s) 280, 298, 310
LpnPI CCDG 9 cut(s) 29, 60, 189, 214, 291, 309, 321, 480, 535
Lsp1109I GCAGC 2 cut(s) 284, 629
MaeI CTAG 1 cut(s) 398
MaeII ACGT 1 cut(s) 610
MalI GATC 1 cut(s) 575
MboI GATC 1 cut(s) 573
MboII GAAGA 7 cut(s) 133, 347, 425, 454, 510, 580, 583
MhlI GDGCHC 1 cut(s) 390
MlsI TGGCCA 1 cut(s) 29
MluCI AATT 4 cut(s) 68, 100, 192, 451
MluNI TGGCCA 1 cut(s) 29
MlyI GAGTC 1 cut(s) 495
MnlI CCTC 7 cut(s) 51, 163, 256, 331, 333, 387, 500
Mox20I TGGCCA 1 cut(s) 29
MroXI GAANNNNTTC 1 cut(s) 552
MscI TGGCCA 1 cut(s) 29
MslI CAYNNNNRTG 1 cut(s) 635
Msp20I TGGCCA 1 cut(s) 29
MspA1I CMGCKG 1 cut(s) 251
MspI CCGG 1 cut(s) 296
MwoI GCNNNNNNNGC 3 cut(s) 206, 278, 531
NdeII GATC 1 cut(s) 573
PdmI GAANNNNTTC 1 cut(s) 552
PfeI GAWTC 2 cut(s) 218, 627
PkrI GCNGC 5 cut(s) 250, 274, 318, 527, 619
PleI GAGTC 1 cut(s) 494
PpsI GAGTC 1 cut(s) 494
Psp124BI GAGCTC 1 cut(s) 390
Psp1406I AACGTT 1 cut(s) 610
PspPI GGNCC 1 cut(s) 497
RsaI GTAC 2 cut(s) 79, 458
RsaNI GTAC 2 cut(s) 78, 457
RseI CAYNNNNRTG 1 cut(s) 635
SacI GAGCTC 1 cut(s) 390
SatI GCNGC 5 cut(s) 249, 273, 317, 526, 618
Sau3AI GATC 1 cut(s) 573
Sau96I GGNCC 1 cut(s) 497
SchI GAGTC 1 cut(s) 495
SduI GDGCHC 1 cut(s) 390
SinI GGWCC 1 cut(s) 497
SmiMI CAYNNNNRTG 1 cut(s) 635
SmlI CTYRAG 1 cut(s) 621
SmoI CTYRAG 1 cut(s) 621
Sse9I AATT 4 cut(s) 68, 100, 192, 451
SsiI CCGC 4 cut(s) 85, 249, 317, 525
SspI AATATT 1 cut(s) 16
SspMI CTAG 1 cut(s) 398
SstI GAGCTC 1 cut(s) 390
TaaI ACNGT 2 cut(s) 244, 544
TaiI ACGT 1 cut(s) 613
TaqI TCGA 1 cut(s) 369
TasI AATT 4 cut(s) 68, 100, 192, 451
TauI GCSGC 3 cut(s) 251, 319, 528
TfiI GAWTC 2 cut(s) 218, 627
TscAI CASTG 2 cut(s) 247, 547
TseI GCWGC 2 cut(s) 272, 617
TspDTI ATGAA 1 cut(s) 219
TspRI CASTG 2 cut(s) 247, 547
VpaK11BI GGWCC 1 cut(s) 497
XapI RAATTY 1 cut(s) 68
XmnI GAANNNNTTC 1 cut(s) 552
XspI CTAG 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.