MD15G1141400.v1.1

Protein of unknown function (DUF2996)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006674
Physical Location & Seq
Forward (+)
10429297 .. 10433331
4035 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1141400.v1.1.491

Sequence Viewer

Length: 495 bp
ATGGCAGGGATTCATTGCCTTGTGATTACATCTTTTTGCCCCGTTTACGTACTAGCTGATGCTGATGCTGCTGATCCAAGTACGAAGGAGGTTAAAACAGCTGATCAAAAAGCAGCAGCTCCGGCGAAGCCTAAAGTTGCAGCGAAAGCTCCAGTGAAGGCGTTGCCTCAAATGATGGAGGAGGACGTTATCCCGCCATTGAAAGCAATACTCCAAACTCAAGATGAACTCTCTAATATTGAGCTTTGTTTTCAAGACAACAGGCTGGAAGGTTCCTTTATAAAGAAGGGCAATCCCTACTCGTTTTGGGTCTTCTTTCCCAGTGGAGTCCTCGCAGGTCCAAAGGGGTTTTCGCTGTCTTCGTATGGCTCAGAAGCGAGCACTGTTGAGCCTTTTCTCGTTGATGAGAAGAAAATCACAGCAAAACATATTGTCTTTTGGGTTGAAAAGCGTTTAGCCGCTCAAGGAATCATTCCTGTCTGGAAATTAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2996

Protein Analysis

165

Amino Acids

17.86

Weight (kDa)

6.13

Isoelectric Point (pI)

38.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2996 PF11210 56 - 155 2.3e-13 Protein of unknown function (DUF2996)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 281
Acc36I ACCTGC 1 cut(s) 326
AccBSI CCGCTC 1 cut(s) 461
AciI CCGC 2 cut(s) 194, 459
AclWI GGATC 1 cut(s) 68
AfaI GTAC 2 cut(s) 51, 82
AgsI TTSAA 3 cut(s) 202, 254, 446
AjuI GAANNNNNNNTTGG 2 cut(s) 334, 366
AluBI AGCT 5 cut(s) 56, 101, 119, 149, 244
AluI AGCT 5 cut(s) 56, 101, 119, 149, 244
Alw21I GWGCWC 1 cut(s) 383
AlwI GGATC 1 cut(s) 68
ApeKI GCWGC 4 cut(s) 68, 113, 116, 140
AspS9I GGNCC 1 cut(s) 338
AvaII GGWCC 1 cut(s) 338
BbsI GAAGAC 2 cut(s) 304, 351
Bbv12I GWGCWC 1 cut(s) 383
BbvI GCAGC 4 cut(s) 55, 125, 128, 152
BccI CCATC 1 cut(s) 169
BclI TGATCA 1 cut(s) 103
BfaI CTAG 1 cut(s) 53
BfuAI ACCTGC 1 cut(s) 326
BisI GCNGC 5 cut(s) 69, 114, 117, 141, 459
BlsI GCNGC 5 cut(s) 70, 115, 118, 142, 460
Bme18I GGWCC 1 cut(s) 338
BmgT120I GGNCC 1 cut(s) 338
BmiI GGNNCC 1 cut(s) 274
BmrI ACTGGG 1 cut(s) 315
BmsI GCATC 2 cut(s) 49, 55
BmuI ACTGGG 1 cut(s) 315
BpiI GAAGAC 2 cut(s) 304, 351
BpmI CTGGAG 1 cut(s) 135
BpuEI CTTGAG 2 cut(s) 204, 447
BsaAI YACGTR 1 cut(s) 49
Bse1I ACTGG 2 cut(s) 152, 321
Bse3DI GCAATG 1 cut(s) 13
BseMI GCAATG 1 cut(s) 13
BseMII CTCAG 1 cut(s) 384
BseNI ACTGG 2 cut(s) 152, 321
BseRI GAGGAG 1 cut(s) 194
BseXI GCAGC 4 cut(s) 55, 125, 128, 152
BsiHKAI GWGCWC 1 cut(s) 383
BsiSI CCGG 1 cut(s) 122
Bsp1286I GDGCHC 1 cut(s) 383
Bsp143I GATC 2 cut(s) 73, 103
BspACI CCGC 2 cut(s) 194, 459
BspCNI CTCAG 1 cut(s) 383
BspLI GGNNCC 1 cut(s) 274
BspMI ACCTGC 1 cut(s) 326
BspPI GGATC 1 cut(s) 68
BsrBI CCGCTC 1 cut(s) 461
BsrDI GCAATG 1 cut(s) 13
BsrI ACTGG 2 cut(s) 152, 321
BssMI GATC 2 cut(s) 73, 103
Bst4CI ACNGT 1 cut(s) 385
BstBAI YACGTR 1 cut(s) 49
BstC8I GCNNGC 1 cut(s) 379
BstDEI CTNAG 1 cut(s) 370
BstKTI GATC 2 cut(s) 76, 106
BstMBI GATC 2 cut(s) 73, 103
BstMWI GCNNNNNNNGC 3 cut(s) 68, 122, 146
BstSNI TACGTA 1 cut(s) 49
BstV1I GCAGC 4 cut(s) 55, 125, 128, 152
BstV2I GAAGAC 2 cut(s) 304, 351
BtsIMutI CAGTG 3 cut(s) 159, 328, 381
BveI ACCTGC 1 cut(s) 326
Cac8I GCNNGC 1 cut(s) 379
Cfr13I GGNCC 1 cut(s) 338
Csp6I GTAC 2 cut(s) 50, 81
CviQI GTAC 2 cut(s) 50, 81
DdeI CTNAG 1 cut(s) 370
DpnI GATC 2 cut(s) 75, 105
DpnII GATC 2 cut(s) 73, 103
Eco105I TACGTA 1 cut(s) 49
Eco47I GGWCC 1 cut(s) 338
FaiI YATR 3 cut(s) 281, 366, 429
FauI CCCGC 1 cut(s) 201
FbaI TGATCA 1 cut(s) 103
Fnu4HI GCNGC 5 cut(s) 69, 114, 117, 141, 459
Fsp4HI GCNGC 5 cut(s) 69, 114, 117, 141, 459
FspBI CTAG 1 cut(s) 53
GluI GCNGC 5 cut(s) 69, 114, 117, 141, 459
GsuI CTGGAG 1 cut(s) 135
HapII CCGG 1 cut(s) 122
HinfI GANTC 3 cut(s) 10, 327, 468
HpaII CCGG 1 cut(s) 122
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 1 cut(s) 373
Hpy188III TCNNGA 3 cut(s) 221, 254, 481
Hpy8I GTNNAC 1 cut(s) 46
HpyAV CCTTC 4 cut(s) 79, 151, 263, 280
HpyCH4III ACNGT 1 cut(s) 385
HpyCH4IV ACGT 2 cut(s) 48, 186
HpyCH4V TGCA 1 cut(s) 140
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 122, 146
HpyF3I CTNAG 1 cut(s) 370
HpySE526I ACGT 2 cut(s) 48, 186
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 2 cut(s) 73, 103
LmnI GCTCC 2 cut(s) 124, 154
LpnPI CCDG 8 cut(s) 135, 165, 247, 251, 321, 334, 466, 489
Lsp1109I GCAGC 4 cut(s) 55, 125, 128, 152
LweI GCATC 2 cut(s) 49, 55
MaeI CTAG 1 cut(s) 53
MaeII ACGT 2 cut(s) 48, 186
MalI GATC 2 cut(s) 75, 105
MbiI CCGCTC 1 cut(s) 461
MboI GATC 2 cut(s) 73, 103
MboII GAAGA 3 cut(s) 304, 351, 421
MhlI GDGCHC 1 cut(s) 383
MluCI AATT 1 cut(s) 485
MlyI GAGTC 1 cut(s) 336
MnlI CCTC 5 cut(s) 82, 172, 175, 177, 341
MseI TTAA 1 cut(s) 93
MspA1I CMGCKG 1 cut(s) 101
MspI CCGG 1 cut(s) 122
MwoI GCNNNNNNNGC 3 cut(s) 68, 122, 146
NdeII GATC 2 cut(s) 73, 103
NlaIV GGNNCC 1 cut(s) 274
PcsI WCGNNNNNNNCGW 1 cut(s) 359
PfeI GAWTC 2 cut(s) 10, 468
PkrI GCNGC 5 cut(s) 70, 115, 118, 142, 460
PleI GAGTC 1 cut(s) 335
PpsI GAGTC 1 cut(s) 335
Ppu21I YACGTR 1 cut(s) 49
PsiI TTATAA 1 cut(s) 281
PspN4I GGNNCC 1 cut(s) 274
PspPI GGNCC 1 cut(s) 338
PvuII CAGCTG 1 cut(s) 101
RsaI GTAC 2 cut(s) 51, 82
RsaNI GTAC 2 cut(s) 50, 81
SaqAI TTAA 1 cut(s) 93
SatI GCNGC 5 cut(s) 69, 114, 117, 141, 459
Sau3AI GATC 2 cut(s) 73, 103
Sau96I GGNCC 1 cut(s) 338
SchI GAGTC 1 cut(s) 336
SduI GDGCHC 1 cut(s) 383
SfaNI GCATC 2 cut(s) 49, 55
SinI GGWCC 1 cut(s) 338
SmlI CTYRAG 2 cut(s) 219, 462
SmoI CTYRAG 2 cut(s) 219, 462
SnaBI TACGTA 1 cut(s) 49
Sse9I AATT 1 cut(s) 485
SsiI CCGC 2 cut(s) 194, 459
SspI AATATT 1 cut(s) 238
SspMI CTAG 1 cut(s) 53
TaaI ACNGT 1 cut(s) 385
TaiI ACGT 2 cut(s) 51, 189
TasI AATT 1 cut(s) 485
TauI GCSGC 1 cut(s) 461
TfiI GAWTC 2 cut(s) 10, 468
Tru1I TTAA 1 cut(s) 93
Tru9I TTAA 1 cut(s) 93
TscAI CASTG 3 cut(s) 159, 328, 388
TseI GCWGC 4 cut(s) 68, 113, 116, 140
TspDTI ATGAA 1 cut(s) 240
TspRI CASTG 3 cut(s) 159, 328, 388
VpaK11BI GGWCC 1 cut(s) 338
XspI CTAG 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.