AT2G04220

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0011855
Physical Location & Seq
Forward (+)
1445085 .. 1446507
1423 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G04220.1

Sequence Viewer

Length: 924 bp
ATGTCATTTAGATCACATTCGAGAGGGAGTAGTCAATTCGCAGAGAAGGTAACAGAGGATCCTGTAACGTACAAGACAGCACAAAGCACCGTGACGTGCATTTACCAAGCGCATATATCCGGATTCTGGAGGAATGTTACGGTATTGTGGTCAAAGAATCTCATGAATCATTCGCTTATGGTCATGGTGACAAACGTGGAAGGCGATATGAATTATTGTTGCAAGGTTGATCTGAAGCCTTGGCATTTTTGGAACAAGAAAGGCTACAAGTCGTTCGATGTGGAAGGTAACCCGGTAGAGGTGTATTGGGATTTTAGGTCTGCGAAATTCACGAGTAGTCCAGAGCCGAGCTCGGATTTCTACGTGGCTTTAGTCTCGGAGGAAGAGGTGGTTTTGCTTGTGGGTGATTACAAGAAGAAAGCTTTCAAGAGAACAAAGTCGAGGCCTGCTTTGGTCGAGGCTGCCTTGTTCTACAAGAAAGAAAACGTGTTTGGCAAGAAGTGTTTCACCACCAGGGCTAAGTTTTACGATAGGAAAAAAGAACACGAGATCATCGTGGAAAGCTCGACCTCCGGGCCTAAAGAGCCAGAGATGTGGATTAGCATAGATGGGATTGTGTTGATACAAGTCAAGAATTTGCAATGGAAATTCAGAGGGAATCAGACAGTGTTGGTAGATAAACAACCTGTTCAAGTGTTTTGGGACGTGTATGATTGGTTGTTCAGCATGCCTGGGACAGGCCATGGTTTGTTCATATTCAAACCAGGGACCACCGAAGACAGCGACATGGAAGGAAGTGGTCACGGTGGTGGTGGTGGTGGTGAGAGCGATACAAGTACAGGGAGCAGGTATTATTCGACCAAGAGTAGTAACCCTTGGCCGCCAGAGTTTTGCCTTTTTCTTCATGCATACAAACTTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

307

Amino Acids

35.08

Weight (kDa)

8.13

Isoelectric Point (pI)

40.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 27 - 305 1.1e-107 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011855)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04220 AT4G12690 AT4G12690 AT5G48270
fragaria_vesca FvH4_6g40240
malus_domestica MD09G1133700.v1.1 MD17G1121100.v1.1 MD17G1122500.v1.1
prunus_persica Prupe.3G192800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0155261
rosa_laevigata RLG00000020831
rosa_multiflora Rmu_sc0012066.1_g000008
rosa_roxburghii Rroxscaffold_2G00093820
rosa_rugosa Rorug02G0446400
rosa_samantha Rh2AG512900 Rh2BG523500 Rh2CG497900 Rh2DG533200
rosa_wichuraiana Rw2G042140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 837
AccIII TCCGGA 1 cut(s) 119
AciI CCGC 1 cut(s) 881
AclWI GGATC 2 cut(s) 53, 66
AcoI YGGCCR 1 cut(s) 878
AcsI RAATTY 3 cut(s) 326, 634, 647
AcuI CTGAAG 1 cut(s) 254
AfaI GTAC 2 cut(s) 71, 838
AfiI CCNNNNNNNGG 3 cut(s) 126, 298, 737
AflIII ACRYGT 2 cut(s) 486, 705
AgsI TTSAA 3 cut(s) 427, 692, 760
AjiI CACGTC 2 cut(s) 96, 706
AjnI CCWGG 3 cut(s) 512, 730, 763
AjuI GAANNNNNNNTTGG 2 cut(s) 474, 506
AleI CACNNNNGTG 1 cut(s) 807
AluBI AGCT 3 cut(s) 351, 422, 564
AluI AGCT 3 cut(s) 351, 422, 564
Alw21I GWGCWC 1 cut(s) 353
Alw26I GTCTC 1 cut(s) 379
AlwI GGATC 2 cut(s) 53, 66
Aor13HI TCCGGA 1 cut(s) 119
AoxI GGCC 4 cut(s) 443, 575, 739, 878
ApeKI GCWGC 1 cut(s) 461
ApoI RAATTY 3 cut(s) 326, 634, 647
Asp700I GAANNNNTTC 2 cut(s) 422, 503
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 2 cut(s) 575, 768
AsuC2I CCSGG 2 cut(s) 293, 574
AsuHPI GGTGA 4 cut(s) 199, 416, 499, 833
AvaII GGWCC 1 cut(s) 768
BamHI GGATCC 1 cut(s) 58
BanII GRGCYC 1 cut(s) 353
BauI CACGAG 2 cut(s) 331, 545
BbsI GAAGAC 1 cut(s) 783
Bbv12I GWGCWC 1 cut(s) 353
BbvI GCAGC 1 cut(s) 448
BccI CCATC 1 cut(s) 602
BciT130I CCWGG 3 cut(s) 514, 732, 765
BcnI CCSGG 2 cut(s) 293, 574
BcoDI GTCTC 1 cut(s) 379
BfuAI ACCTGC 1 cut(s) 837
BisI GCNGC 2 cut(s) 462, 881
BlsI GCNGC 2 cut(s) 463, 882
Bme1390I CCNGG 5 cut(s) 293, 514, 574, 732, 765
Bme18I GGWCC 1 cut(s) 768
BmgBI CACGTC 2 cut(s) 96, 706
BmgT120I GGNCC 2 cut(s) 575, 768
BmiI GGNNCC 2 cut(s) 60, 769
BmrFI CCNGG 5 cut(s) 293, 514, 574, 732, 765
BpiI GAAGAC 1 cut(s) 783
BplI GAGNNNNNCTC 2 cut(s) 335, 367
BpmI CTGGAG 1 cut(s) 148
BpuMI CCSGG 2 cut(s) 293, 574
BsaAI YACGTR 1 cut(s) 364
BsaJI CCNNGG 6 cut(s) 239, 513, 731, 742, 764, 875
BsaWI WCCGGW 1 cut(s) 119
Bsc4I CCNNNNNNNGG 3 cut(s) 126, 298, 737
Bse3DI GCAATG 1 cut(s) 647
BseAI TCCGGA 1 cut(s) 119
BseBI CCWGG 3 cut(s) 514, 732, 765
BseDI CCNNGG 6 cut(s) 239, 513, 731, 742, 764, 875
BseLI CCNNNNNNNGG 3 cut(s) 126, 298, 737
BseMI GCAATG 1 cut(s) 647
BseXI GCAGC 1 cut(s) 448
BshFI GGCC 4 cut(s) 445, 577, 741, 880
BsiHKAI GWGCWC 1 cut(s) 353
BsiSI CCGG 3 cut(s) 120, 293, 573
BslFI GGGAC 3 cut(s) 716, 748, 781
BslI CCNNNNNNNGG 3 cut(s) 126, 298, 737
BsmAI GTCTC 1 cut(s) 379
BsmFI GGGAC 3 cut(s) 716, 748, 781
BsnI GGCC 4 cut(s) 445, 577, 741, 880
Bsp1286I GDGCHC 1 cut(s) 353
Bsp13I TCCGGA 1 cut(s) 119
Bsp143I GATC 4 cut(s) 11, 58, 229, 549
Bsp19I CCATGG 1 cut(s) 742
BspACI CCGC 1 cut(s) 881
BspANI GGCC 4 cut(s) 445, 577, 741, 880
BspEI TCCGGA 1 cut(s) 119
BspHI TCATGA 1 cut(s) 162
BspLI GGNNCC 2 cut(s) 60, 769
BspMI ACCTGC 1 cut(s) 837
BspPI GGATC 2 cut(s) 53, 66
BsrDI GCAATG 1 cut(s) 647
BssECI CCNNGG 6 cut(s) 239, 513, 731, 742, 764, 875
BssMI GATC 4 cut(s) 11, 58, 229, 549
BssSI CACGAG 2 cut(s) 331, 545
BssT1I CCWWGG 3 cut(s) 239, 742, 875
Bst2BI CACGAG 2 cut(s) 331, 545
Bst2UI CCWGG 3 cut(s) 514, 732, 765
Bst4CI ACNGT 4 cut(s) 91, 142, 667, 806
Bst6I CTCTTC 1 cut(s) 378
BstBAI YACGTR 1 cut(s) 364
BstC8I GCNNGC 2 cut(s) 447, 728
BstDEI CTNAG 1 cut(s) 519
BstDSI CCRYGG 1 cut(s) 742
BstEII GGTNACC 1 cut(s) 287
BstENI CCTNNNNNAGG 1 cut(s) 735
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 4 cut(s) 14, 61, 232, 552
BstMAI GTCTC 1 cut(s) 379
BstMBI GATC 4 cut(s) 11, 58, 229, 549
BstMWI GCNNNNNNNGC 1 cut(s) 583
BstNI CCWGG 3 cut(s) 514, 732, 765
BstNSI RCATGY 1 cut(s) 730
BstPI GGTNACC 1 cut(s) 287
BstSCI CCNGG 5 cut(s) 291, 512, 572, 730, 763
BstV1I GCAGC 1 cut(s) 448
BstV2I GAAGAC 1 cut(s) 783
BstX2I RGATCY 1 cut(s) 58
BstXI CCANNNNNNTGG 1 cut(s) 594
BstYI RGATCY 1 cut(s) 58
BsuRI GGCC 4 cut(s) 445, 577, 741, 880
BtgI CCRYGG 1 cut(s) 742
BtrI CACGTC 2 cut(s) 96, 706
BtsIMutI CAGTG 1 cut(s) 672
BveI ACCTGC 1 cut(s) 837
Cac8I GCNNGC 2 cut(s) 447, 728
CciI TCATGA 1 cut(s) 162
CfoI GCGC 1 cut(s) 112
Cfr13I GGNCC 2 cut(s) 575, 768
Csp6I GTAC 2 cut(s) 70, 837
CviAII CATG 6 cut(s) 163, 184, 727, 743, 787, 905
CviQI GTAC 2 cut(s) 70, 837
DdeI CTNAG 1 cut(s) 519
DpnI GATC 4 cut(s) 13, 60, 231, 551
DpnII GATC 4 cut(s) 11, 58, 229, 549
EaeI YGGCCR 1 cut(s) 878
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
Ecl136II GAGCTC 1 cut(s) 351
Eco130I CCWWGG 3 cut(s) 239, 742, 875
Eco147I AGGCCT 1 cut(s) 445
Eco24I GRGCYC 1 cut(s) 353
Eco47I GGWCC 1 cut(s) 768
Eco53kI GAGCTC 1 cut(s) 351
Eco57I CTGAAG 1 cut(s) 254
Eco91I GGTNACC 1 cut(s) 287
EcoICRI GAGCTC 1 cut(s) 351
EcoNI CCTNNNNNAGG 1 cut(s) 735
EcoO65I GGTNACC 1 cut(s) 287
EcoRII CCWGG 3 cut(s) 512, 730, 763
EcoT14I CCWWGG 3 cut(s) 239, 742, 875
EcoT22I ATGCAT 1 cut(s) 910
EcoT38I GRGCYC 1 cut(s) 353
ErhI CCWWGG 3 cut(s) 239, 742, 875
FaeI CATG 6 cut(s) 166, 187, 730, 746, 790, 908
FaqI GGGAC 3 cut(s) 716, 748, 781
FatI CATG 6 cut(s) 162, 183, 726, 742, 786, 904
Fnu4HI GCNGC 2 cut(s) 462, 881
FriOI GRGCYC 1 cut(s) 353
Fsp4HI GCNGC 2 cut(s) 462, 881
GlaI GCGC 1 cut(s) 111
GluI GCNGC 2 cut(s) 462, 881
GsuI CTGGAG 1 cut(s) 148
HaeIII GGCC 4 cut(s) 445, 577, 741, 880
HapII CCGG 3 cut(s) 120, 293, 573
HhaI GCGC 1 cut(s) 112
Hin1II CATG 6 cut(s) 166, 187, 730, 746, 790, 908
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HindIII AAGCTT 1 cut(s) 420
HinfI GANTC 4 cut(s) 123, 157, 166, 658
HpaII CCGG 3 cut(s) 120, 293, 573
HphI GGTGA 4 cut(s) 199, 416, 499, 833
Hpy188I TCNGA 5 cut(s) 234, 355, 379, 653, 663
Hpy188III TCNNGA 8 cut(s) 21, 120, 127, 163, 331, 341, 427, 631
HpyAV CCTTC 4 cut(s) 40, 194, 278, 785
HpyCH4III ACNGT 4 cut(s) 91, 142, 667, 806
HpyCH4IV ACGT 6 cut(s) 68, 95, 195, 363, 486, 705
HpyCH4V TGCA 4 cut(s) 99, 222, 640, 908
HpyF10VI GCNNNNNNNGC 1 cut(s) 583
HpyF3I CTNAG 1 cut(s) 519
HpySE526I ACGT 6 cut(s) 68, 95, 195, 363, 486, 705
Hsp92II CATG 6 cut(s) 166, 187, 730, 746, 790, 908
HspAI GCGC 1 cut(s) 110
Kpn2I TCCGGA 1 cut(s) 119
Kzo9I GATC 4 cut(s) 11, 58, 229, 549
LmnI GCTCC 1 cut(s) 843
Lsp1109I GCAGC 1 cut(s) 448
MaeII ACGT 6 cut(s) 68, 95, 195, 363, 486, 705
MaeIII GTNAC 8 cut(s) 49, 64, 91, 136, 187, 287, 800, 869
MalI GATC 4 cut(s) 13, 60, 231, 551
MboI GATC 4 cut(s) 11, 58, 229, 549
MboII GAAGA 4 cut(s) 395, 427, 788, 893
MflI RGATCY 1 cut(s) 58
MhlI GDGCHC 1 cut(s) 353
MluCI AATT 5 cut(s) 35, 211, 326, 634, 647
Mph1103I ATGCAT 1 cut(s) 910
MroI TCCGGA 1 cut(s) 119
MroXI GAANNNNTTC 2 cut(s) 422, 503
MslI CAYNNNNRTG 1 cut(s) 807
MspI CCGG 3 cut(s) 120, 293, 573
MspR9I CCNGG 5 cut(s) 293, 514, 574, 732, 765
MvaI CCWGG 3 cut(s) 514, 732, 765
MwoI GCNNNNNNNGC 1 cut(s) 583
NciI CCSGG 2 cut(s) 293, 574
NcoI CCATGG 1 cut(s) 742
NdeII GATC 4 cut(s) 11, 58, 229, 549
NlaIII CATG 6 cut(s) 166, 187, 730, 746, 790, 908
NlaIV GGNNCC 2 cut(s) 60, 769
NmeAIII GCCGAG 1 cut(s) 372
NmuCI GTSAC 3 cut(s) 91, 187, 800
NsiI ATGCAT 1 cut(s) 910
NspI RCATGY 1 cut(s) 730
OliI CACNNNNGTG 1 cut(s) 807
PaeI GCATGC 1 cut(s) 730
PagI TCATGA 1 cut(s) 162
PceI AGGCCT 1 cut(s) 445
PcsI WCGNNNNNNNCGW 2 cut(s) 201, 552
PdmI GAANNNNTTC 2 cut(s) 422, 503
PfeI GAWTC 4 cut(s) 123, 157, 166, 658
PkrI GCNGC 2 cut(s) 463, 882
Ppu21I YACGTR 1 cut(s) 364
Psp124BI GAGCTC 1 cut(s) 353
Psp6I CCWGG 3 cut(s) 512, 730, 763
PspEI GGTNACC 1 cut(s) 287
PspGI CCWGG 3 cut(s) 512, 730, 763
PspN4I GGNNCC 2 cut(s) 60, 769
PspPI GGNCC 2 cut(s) 575, 768
PsuI RGATCY 1 cut(s) 58
RsaI GTAC 2 cut(s) 71, 838
RsaNI GTAC 2 cut(s) 70, 837
RseI CAYNNNNRTG 1 cut(s) 807
SacI GAGCTC 1 cut(s) 353
SatI GCNGC 2 cut(s) 462, 881
Sau3AI GATC 4 cut(s) 11, 58, 229, 549
Sau96I GGNCC 2 cut(s) 575, 768
ScrFI CCNGG 5 cut(s) 293, 514, 574, 732, 765
SduI GDGCHC 1 cut(s) 353
SinI GGWCC 1 cut(s) 768
SmiMI CAYNNNNRTG 1 cut(s) 807
SmlI CTYRAG 1 cut(s) 917
SmoI CTYRAG 1 cut(s) 917
SphI GCATGC 1 cut(s) 730
Sse9I AATT 5 cut(s) 35, 211, 326, 634, 647
SseBI AGGCCT 1 cut(s) 445
SsiI CCGC 1 cut(s) 881
SstI GAGCTC 1 cut(s) 353
StuI AGGCCT 1 cut(s) 445
StyD4I CCNGG 5 cut(s) 291, 512, 572, 730, 763
StyI CCWWGG 3 cut(s) 239, 742, 875
TaaI ACNGT 4 cut(s) 91, 142, 667, 806
TaiI ACGT 6 cut(s) 71, 98, 198, 366, 489, 708
TaqI TCGA 6 cut(s) 20, 276, 440, 456, 566, 857
TasI AATT 5 cut(s) 35, 211, 326, 634, 647
TatI WGTACW 1 cut(s) 836
TauI GCSGC 1 cut(s) 883
TfiI GAWTC 4 cut(s) 123, 157, 166, 658
TscAI CASTG 1 cut(s) 672
TseFI GTSAC 3 cut(s) 91, 187, 800
TseI GCWGC 1 cut(s) 461
Tsp45I GTSAC 3 cut(s) 91, 187, 800
TspDTI ATGAA 4 cut(s) 179, 224, 742, 893
TspRI CASTG 1 cut(s) 672
VpaK11BI GGWCC 1 cut(s) 768
XagI CCTNNNNNAGG 1 cut(s) 735
XapI RAATTY 3 cut(s) 326, 634, 647
XceI RCATGY 1 cut(s) 730
XmnI GAANNNNTTC 2 cut(s) 422, 503
Zsp2I ATGCAT 1 cut(s) 910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.