MD09G1133700.v1.1

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011855
Physical Location & Seq
Forward (+)
10270977 .. 10271909
933 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1133700.v1.1.491

Sequence Viewer

Length: 933 bp
ATGCAGTCTTTGAGTCGATCGATGCGAGGTAAATCATTTCATGAGTCTCCGCCATTTGCAGAGAAGATCACAGACGACCCTGTGACAACGAAAACCGCACAAAGCACAGTGACTTGCGTCTTCCTTGCCAATATTGCGACATTTTGGCGGAAGGTGACGATCATCTGGTGTAAGAACCTCATGAACCATTCCCTCAGCATTCGGATTGATAGCTTAGACGGCGAGTTTCATTATACGTGCAAATTCGACCTAAAGCCATGGCATTTTTGGAGCAAAAAAGGGTACAAGTCATTCGAGTTCGAAGGGAATCAAGTGGAGGTTTATTGGGATCTTCGGTCTGCGAAATTTGGCGGCGGCCCAGAACCGTGTTCCGACTTTTATGTTGCTCTTGTTTGCGATGAGGAGGTTGTGTTGTTATTGGGAGACATGAAGAAAAAGGCATACAAGAGAACAAAATCCAGACCAGCCCTTGTGGAGGCACTTTTATACTACAAAAAAGAAAACGTGTTTGCCAAGAAAAGTTTTGCCACAAGAGTGAAGTTTGATGAGAAGAGAAAAGAGCATGACATTGTGGTGGAGAGCTCAACGTCCGGCCTGAAAGACCCAGAAATGTGGATCAGTGTAGACGGGATTGTGTTGATTCATGTCAAGAATTTGCAGTGGAAATTCAGAGGGAATCAGACCGTGCAGGTGAACATGCAACCGGTGCAAGTTTTCTGGGACGTGCACGATTGGCTTTTCAGCAGCCCGGGGACGGGGCATGGGCTGTTTATTTTCAAGCCAGGGGCACCGGAATCAGAGAGCGATCACGACAGAGATGGCAACGGCAGCATAAACAGTGATAATAGCAGTGCTTATTATTCAACCCAGACAAGTAATAACTCAACATCTTCTGACTTCTGCATGTTTCTTCATGCTTATAAGATTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

311

Amino Acids

35.54

Weight (kDa)

7.64

Isoelectric Point (pI)

35.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 33 - 308 2.3e-108 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011855)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04220 AT4G12690 AT4G12690 AT5G48270
fragaria_vesca FvH4_6g40240
malus_domestica MD09G1133700.v1.1 MD17G1121100.v1.1 MD17G1122500.v1.1
prunus_persica Prupe.3G192800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0155261
rosa_laevigata RLG00000020831
rosa_multiflora Rmu_sc0012066.1_g000008
rosa_roxburghii Rroxscaffold_2G00093820
rosa_rugosa Rorug02G0446400
rosa_samantha Rh2AG512900 Rh2BG523500 Rh2CG497900 Rh2DG533200
rosa_wichuraiana Rw2G042140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 921
AarI CACCTGC 1 cut(s) 679
Acc36I ACCTGC 1 cut(s) 679
AccB1I GGYRCC 1 cut(s) 787
AccI GTMKAC 1 cut(s) 624
AciI CCGC 5 cut(s) 50, 96, 148, 351, 354
AclWI GGATC 2 cut(s) 336, 623
AcsI RAATTY 4 cut(s) 242, 344, 652, 665
AfaI GTAC 1 cut(s) 284
AfiI CCNNNNNNNGG 3 cut(s) 475, 754, 755
AflIII ACRYGT 1 cut(s) 504
AgeI ACCGGT 1 cut(s) 703
AgsI TTSAA 2 cut(s) 778, 864
AjiI CACGTC 1 cut(s) 724
AjnI CCWGG 1 cut(s) 781
AleI CACNNNNGTG 1 cut(s) 533
AluBI AGCT 2 cut(s) 213, 582
AluI AGCT 2 cut(s) 213, 582
Alw21I GWGCWC 2 cut(s) 584, 729
Alw26I GTCTC 2 cut(s) 51, 417
Alw44I GTGCAC 1 cut(s) 725
AlwI GGATC 2 cut(s) 336, 623
Ama87I CYCGRG 1 cut(s) 748
AoxI GGCC 2 cut(s) 355, 592
ApaLI GTGCAC 1 cut(s) 725
ApeKI GCWGC 2 cut(s) 744, 828
ApoI RAATTY 4 cut(s) 242, 344, 652, 665
AsiGI ACCGGT 1 cut(s) 703
AspS9I GGNCC 1 cut(s) 356
AsuC2I CCSGG 2 cut(s) 749, 750
AsuHPI GGTGA 2 cut(s) 166, 703
AsuII TTCGAA 1 cut(s) 300
AvaI CYCGRG 1 cut(s) 748
BaeGI GKGCMC 2 cut(s) 729, 790
BanI GGYRCC 1 cut(s) 787
BanII GRGCYC 1 cut(s) 584
BbsI GAAGAC 1 cut(s) 112
Bbv12I GWGCWC 2 cut(s) 584, 729
BbvCI CCTCAGC 1 cut(s) 194
BbvI GCAGC 2 cut(s) 756, 840
BccI CCATC 1 cut(s) 812
BceAI ACGGC 2 cut(s) 235, 841
BciT130I CCWGG 1 cut(s) 783
BcnI CCSGG 2 cut(s) 749, 750
BcoDI GTCTC 2 cut(s) 51, 417
BfuAI ACCTGC 1 cut(s) 679
BisI GCNGC 4 cut(s) 352, 355, 745, 829
BlsI GCNGC 4 cut(s) 353, 356, 746, 830
Bme1390I CCNGG 3 cut(s) 749, 750, 783
BmeT110I CYCGRG 1 cut(s) 748
BmgBI CACGTC 1 cut(s) 724
BmgT120I GGNCC 1 cut(s) 356
BmiI GGNNCC 1 cut(s) 789
BmrFI CCNGG 3 cut(s) 749, 750, 783
BmsI GCATC 1 cut(s) 12
BoxI GACNNNNGTC 1 cut(s) 116
BpiI GAAGAC 1 cut(s) 112
Bpu10I CCTNAGC 1 cut(s) 194
Bpu14I TTCGAA 1 cut(s) 300
BpuMI CCSGG 2 cut(s) 749, 750
Bsa29I ATCGAT 1 cut(s) 20
BsaAI YACGTR 1 cut(s) 237
BsaJI CCNNGG 4 cut(s) 257, 748, 749, 782
BsaWI WCCGGW 2 cut(s) 703, 790
BsaXI ACNNNNNCTCC 2 cut(s) 395, 425
Bsc4I CCNNNNNNNGG 3 cut(s) 475, 754, 755
Bse118I RCCGGY 1 cut(s) 703
BseBI CCWGG 1 cut(s) 783
BseCI ATCGAT 1 cut(s) 20
BseDI CCNNGG 4 cut(s) 257, 748, 749, 782
BseLI CCNNNNNNNGG 3 cut(s) 475, 754, 755
BseMII CTCAG 1 cut(s) 208
BseRI GAGGAG 1 cut(s) 416
BseSI GKGCMC 2 cut(s) 729, 790
BseXI GCAGC 2 cut(s) 756, 840
BsgI GTGCAG 1 cut(s) 707
Bsh1285I CGRYCG 1 cut(s) 20
BshFI GGCC 2 cut(s) 357, 594
BshNI GGYRCC 1 cut(s) 787
BshTI ACCGGT 1 cut(s) 703
BshVI ATCGAT 1 cut(s) 20
BsiEI CGRYCG 1 cut(s) 20
BsiHKAI GWGCWC 2 cut(s) 584, 729
BsiHKCI CYCGRG 1 cut(s) 748
BsiSI CCGG 4 cut(s) 591, 704, 749, 791
BslFI GGGAC 2 cut(s) 734, 766
BslI CCNNNNNNNGG 3 cut(s) 475, 754, 755
BsmAI GTCTC 2 cut(s) 51, 417
BsmFI GGGAC 2 cut(s) 734, 766
BsmI GAATGC 1 cut(s) 198
BsnI GGCC 2 cut(s) 357, 594
BsoBI CYCGRG 1 cut(s) 748
Bsp119I TTCGAA 1 cut(s) 300
Bsp1286I GDGCHC 3 cut(s) 584, 729, 790
Bsp143I GATC 6 cut(s) 17, 66, 159, 328, 615, 805
Bsp19I CCATGG 1 cut(s) 257
BspACI CCGC 5 cut(s) 50, 96, 148, 351, 354
BspANI GGCC 2 cut(s) 357, 594
BspCNI CTCAG 1 cut(s) 207
BspDI ATCGAT 1 cut(s) 20
BspHI TCATGA 2 cut(s) 40, 180
BspLI GGNNCC 1 cut(s) 789
BspMI ACCTGC 1 cut(s) 679
BspPI GGATC 2 cut(s) 336, 623
BspT104I TTCGAA 1 cut(s) 300
BspT107I GGYRCC 1 cut(s) 787
BsrFI RCCGGY 1 cut(s) 703
BssAI RCCGGY 1 cut(s) 703
BssECI CCNNGG 4 cut(s) 257, 748, 749, 782
BssMI GATC 6 cut(s) 17, 66, 159, 328, 615, 805
BssT1I CCWWGG 1 cut(s) 257
Bst2UI CCWGG 1 cut(s) 783
Bst4CI ACNGT 4 cut(s) 109, 366, 685, 839
Bst6I CTCTTC 1 cut(s) 545
BstAPI GCANNNNNTGC 1 cut(s) 706
BstBAI YACGTR 1 cut(s) 237
BstBI TTCGAA 1 cut(s) 300
BstDEI CTNAG 2 cut(s) 194, 214
BstDSI CCRYGG 1 cut(s) 257
BstENI CCTNNNNNAGG 1 cut(s) 473
BstKTI GATC 6 cut(s) 20, 69, 162, 331, 618, 808
BstMAI GTCTC 2 cut(s) 51, 417
BstMBI GATC 6 cut(s) 17, 66, 159, 328, 615, 805
BstMCI CGRYCG 1 cut(s) 20
BstMWI GCNNNNNNNGC 5 cut(s) 134, 219, 706, 733, 828
BstNI CCWGG 1 cut(s) 783
BstNSI RCATGY 2 cut(s) 700, 907
BstPAI GACNNNNGTC 1 cut(s) 116
BstSCI CCNGG 3 cut(s) 747, 748, 781
BstSLI GKGCMC 2 cut(s) 729, 790
BstV1I GCAGC 2 cut(s) 756, 840
BstV2I GAAGAC 1 cut(s) 112
BstX2I RGATCY 1 cut(s) 328
BstXI CCANNNNNNTGG 1 cut(s) 612
BstYI RGATCY 1 cut(s) 328
Bsu15I ATCGAT 1 cut(s) 20
BsuRI GGCC 2 cut(s) 357, 594
BsuTUI ATCGAT 1 cut(s) 20
BtgI CCRYGG 1 cut(s) 257
BtgZI GCGATG 1 cut(s) 411
BtrI CACGTC 1 cut(s) 724
BtsI GCAGTG 2 cut(s) 665, 856
BtsIMutI CAGTG 5 cut(s) 114, 625, 665, 844, 856
BveI ACCTGC 1 cut(s) 679
CciI TCATGA 2 cut(s) 40, 180
Cfr10I RCCGGY 1 cut(s) 703
Cfr13I GGNCC 1 cut(s) 356
Cfr9I CCCGGG 1 cut(s) 748
ClaI ATCGAT 1 cut(s) 20
CseI GACGC 1 cut(s) 106
Csp6I GTAC 1 cut(s) 283
CspAI ACCGGT 1 cut(s) 703
CviQI GTAC 1 cut(s) 283
DdeI CTNAG 2 cut(s) 194, 214
DpnI GATC 6 cut(s) 19, 68, 161, 330, 617, 807
DpnII GATC 6 cut(s) 17, 66, 159, 328, 615, 805
Eam1104I CTCTTC 1 cut(s) 545
EarI CTCTTC 1 cut(s) 545
EciI GGCGGA 2 cut(s) 39, 163
Ecl136II GAGCTC 1 cut(s) 582
Eco130I CCWWGG 1 cut(s) 257
Eco24I GRGCYC 1 cut(s) 584
Eco53kI GAGCTC 1 cut(s) 582
Eco88I CYCGRG 1 cut(s) 748
EcoICRI GAGCTC 1 cut(s) 582
EcoNI CCTNNNNNAGG 1 cut(s) 473
EcoRII CCWGG 1 cut(s) 781
EcoT14I CCWWGG 1 cut(s) 257
EcoT38I GRGCYC 1 cut(s) 584
ErhI CCWWGG 1 cut(s) 257
FaqI GGGAC 2 cut(s) 734, 766
FblI GTMKAC 1 cut(s) 624
Fnu4HI GCNGC 4 cut(s) 352, 355, 745, 829
FriOI GRGCYC 1 cut(s) 584
Fsp4HI GCNGC 4 cut(s) 352, 355, 745, 829
GluI GCNGC 4 cut(s) 352, 355, 745, 829
HaeIII GGCC 2 cut(s) 357, 594
HapII CCGG 4 cut(s) 591, 704, 749, 791
HgaI GACGC 1 cut(s) 106
HinfI GANTC 6 cut(s) 13, 44, 307, 640, 676, 794
HpaII CCGG 4 cut(s) 591, 704, 749, 791
HphI GGTGA 2 cut(s) 166, 703
Hpy166II GTNNAC 3 cut(s) 625, 694, 727
Hpy188I TCNGA 6 cut(s) 204, 373, 671, 681, 799, 895
Hpy188III TCNNGA 5 cut(s) 41, 181, 459, 649, 809
Hpy8I GTNNAC 3 cut(s) 625, 694, 727
HpyAV CCTTC 2 cut(s) 145, 296
HpyCH4III ACNGT 4 cut(s) 109, 366, 685, 839
HpyCH4IV ACGT 4 cut(s) 236, 504, 587, 723
HpyCH4V TGCA 9 cut(s) 4, 59, 240, 658, 688, 700, 709, 727, 903
HpyF10VI GCNNNNNNNGC 5 cut(s) 134, 219, 706, 733, 828
HpyF3I CTNAG 2 cut(s) 194, 214
HpySE526I ACGT 4 cut(s) 236, 504, 587, 723
Kzo9I GATC 6 cut(s) 17, 66, 159, 328, 615, 805
LmnI GCTCC 1 cut(s) 270
Lsp1109I GCAGC 2 cut(s) 756, 840
LweI GCATC 1 cut(s) 12
MaeII ACGT 4 cut(s) 236, 504, 587, 723
MaeIII GTNAC 3 cut(s) 82, 109, 154
MalI GATC 6 cut(s) 19, 68, 161, 330, 617, 807
MboI GATC 6 cut(s) 17, 66, 159, 328, 615, 805
MboII GAAGA 7 cut(s) 76, 112, 323, 442, 562, 882, 902
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 3 cut(s) 584, 729, 790
MluCI AATT 4 cut(s) 242, 344, 652, 665
MlyI GAGTC 2 cut(s) 22, 53
MmeI TCCRAC 1 cut(s) 396
MnlI CCTC 8 cut(s) 20, 188, 203, 310, 394, 397, 469, 665
MslI CAYNNNNRTG 2 cut(s) 533, 572
MspI CCGG 4 cut(s) 591, 704, 749, 791
MspR9I CCNGG 3 cut(s) 749, 750, 783
Mva1269I GAATGC 1 cut(s) 198
MvaI CCWGG 1 cut(s) 783
MwoI GCNNNNNNNGC 5 cut(s) 134, 219, 706, 733, 828
NciI CCSGG 2 cut(s) 749, 750
NcoI CCATGG 1 cut(s) 257
NdeII GATC 6 cut(s) 17, 66, 159, 328, 615, 805
NlaIV GGNNCC 1 cut(s) 789
NmuCI GTSAC 3 cut(s) 82, 109, 154
NspI RCATGY 2 cut(s) 700, 907
NspV TTCGAA 1 cut(s) 300
OliI CACNNNNGTG 1 cut(s) 533
PagI TCATGA 2 cut(s) 40, 180
PaqCI CACCTGC 1 cut(s) 679
PcsI WCGNNNNNNNCGW 1 cut(s) 22
PctI GAATGC 1 cut(s) 198
PfeI GAWTC 4 cut(s) 307, 640, 676, 794
PinAI ACCGGT 1 cut(s) 703
PkrI GCNGC 4 cut(s) 353, 356, 746, 830
Ple19I CGATCG 1 cut(s) 20
PleI GAGTC 2 cut(s) 21, 52
PpsI GAGTC 2 cut(s) 21, 52
Ppu21I YACGTR 1 cut(s) 237
PshAI GACNNNNGTC 1 cut(s) 116
PsiI TTATAA 1 cut(s) 921
Psp124BI GAGCTC 1 cut(s) 584
Psp6I CCWGG 1 cut(s) 781
PspGI CCWGG 1 cut(s) 781
PspN4I GGNNCC 1 cut(s) 789
PspPI GGNCC 1 cut(s) 356
PsuI RGATCY 1 cut(s) 328
PvuI CGATCG 1 cut(s) 20
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
RseI CAYNNNNRTG 2 cut(s) 533, 572
SacI GAGCTC 1 cut(s) 584
SatI GCNGC 4 cut(s) 352, 355, 745, 829
Sau3AI GATC 6 cut(s) 17, 66, 159, 328, 615, 805
Sau96I GGNCC 1 cut(s) 356
SchI GAGTC 2 cut(s) 22, 53
ScrFI CCNGG 3 cut(s) 749, 750, 783
SduI GDGCHC 3 cut(s) 584, 729, 790
SfaNI GCATC 1 cut(s) 12
SfuI TTCGAA 1 cut(s) 300
SmaI CCCGGG 1 cut(s) 750
SmiMI CAYNNNNRTG 2 cut(s) 533, 572
Sse9I AATT 4 cut(s) 242, 344, 652, 665
SsiI CCGC 5 cut(s) 50, 96, 148, 351, 354
SspI AATATT 1 cut(s) 133
SstI GAGCTC 1 cut(s) 584
StyD4I CCNGG 3 cut(s) 747, 748, 781
StyI CCWWGG 1 cut(s) 257
TaaI ACNGT 4 cut(s) 109, 366, 685, 839
TaiI ACGT 4 cut(s) 239, 507, 590, 726
TaqI TCGA 5 cut(s) 16, 20, 246, 294, 300
TaqII GACCGA 1 cut(s) 324
TasI AATT 4 cut(s) 242, 344, 652, 665
TauI GCSGC 2 cut(s) 354, 357
TfiI GAWTC 4 cut(s) 307, 640, 676, 794
TscAI CASTG 5 cut(s) 114, 625, 665, 844, 856
TseFI GTSAC 3 cut(s) 82, 109, 154
TseI GCWGC 2 cut(s) 744, 828
Tsp45I GTSAC 3 cut(s) 82, 109, 154
TspDTI ATGAA 6 cut(s) 29, 197, 218, 443, 632, 902
TspMI CCCGGG 1 cut(s) 748
TspRI CASTG 5 cut(s) 114, 625, 665, 844, 856
VneI GTGCAC 1 cut(s) 725
XagI CCTNNNNNAGG 1 cut(s) 473
XapI RAATTY 4 cut(s) 242, 344, 652, 665
XceI RCATGY 2 cut(s) 700, 907
XcmI CCANNNNNNNNNTGG 1 cut(s) 264
XmaI CCCGGG 1 cut(s) 748
XmiI GTMKAC 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.