MD17G1121100.v1.1

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0011855
Physical Location & Seq
Forward (+)
10566520 .. 10567507
988 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1121100.v1.1.491

Sequence Viewer

Length: 930 bp
ATGCAGTCTTTGAGTCGATCAATACGAGGTCAATCATTACATGAGCCTCCACCATTTGCAGAGAAGATCACAGAAGACCCTGTGACAACGAAAACCGCACAAAGCACGGTGACATGCGTCTACCTTGCAAATATTGCGACATGTTGGCGGAAGGTGACGATCATATGGTGTAAGAACCTCATGAACCATTCCCTCAGCATTCGGATTGATAGCTTAGACGGCGGTTTTCATTATACGTGCAAAATCGACCTAAAGCCATGGCATTTTTGGAGCAAAAAAGGGTATAAGTCATTCGAGGTCGAAGGGAATCAAGTGGAGGTTTATTGGGATCTTCGTTCTGCAAAATTTGGCGGCGGCCCAGAACCTTGTTCTGACTTTTATGTTGCTCTTGTTTGCGATCAGGAGATTGTGTTGTTATTGGGAGACATGAAGAAAAAGGCATACAAGAGAACAAAATCCAGACCAGCCCTTGTGGAGGCACTTTTATACTACAAAAAAGAAAACGTGTTTGCCAAGAAAAGTTTTGCCACAAGAGTGAAGTTTGATGAGAATAGAAAAGAGCATGACATTGTCGTGGAGAGCTCAACATCGGGCTCGAAAGACCCCGAAATGTGGATCAGCATAGATGGGATTGTGTTGATTCATGTCAAGAATTTGCAGTGGAAATTCAGAGGGAATCAGACCTTGCAGGTGAACATGCAACCGGTGCAAGTTTTCTGGGACGTGCACGATTGGCTTTTCAGCAGCCCCGGGACTGGGCACGGGCTGTTTATTTTCAAGCCAGGGCCGCCGGAATCAGAGAGCGATCACGACAGAGATGGCAACGGCGGCATAGACAGTGATCATAGCAGTCCTTATTATTCAACTCAGACTAGTAACTCAACATCTTCTGACTTCTGCCTGTTTCTTCATGCATATAAGATTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

310

Amino Acids

35.25

Weight (kDa)

7.16

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 33 - 307 1.8e-109 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011855)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04220 AT4G12690 AT4G12690 AT5G48270
fragaria_vesca FvH4_6g40240
malus_domestica MD09G1133700.v1.1 MD17G1121100.v1.1 MD17G1122500.v1.1
prunus_persica Prupe.3G192800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0155261
rosa_laevigata RLG00000020831
rosa_multiflora Rmu_sc0012066.1_g000008
rosa_roxburghii Rroxscaffold_2G00093820
rosa_rugosa Rorug02G0446400
rosa_samantha Rh2AG512900 Rh2BG523500 Rh2CG497900 Rh2DG533200
rosa_wichuraiana Rw2G042140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 679
Acc36I ACCTGC 1 cut(s) 679
AccI GTMKAC 1 cut(s) 120
AciI CCGC 7 cut(s) 96, 148, 222, 351, 354, 788, 828
AclWI GGATC 2 cut(s) 336, 623
AcsI RAATTY 3 cut(s) 344, 652, 665
AfiI CCNNNNNNNGG 4 cut(s) 475, 612, 755, 756
AflIII ACRYGT 2 cut(s) 140, 504
AgeI ACCGGT 1 cut(s) 703
AgsI TTSAA 2 cut(s) 778, 864
AhlI ACTAGT 1 cut(s) 872
AjiI CACGTC 1 cut(s) 724
AjnI CCWGG 1 cut(s) 781
AleI CACNNNNGTG 1 cut(s) 533
AluBI AGCT 2 cut(s) 213, 582
AluI AGCT 2 cut(s) 213, 582
Alw21I GWGCWC 2 cut(s) 584, 729
Alw26I GTCTC 1 cut(s) 417
Alw44I GTGCAC 1 cut(s) 725
AlwI GGATC 2 cut(s) 336, 623
Ama87I CYCGRG 1 cut(s) 749
AoxI GGCC 2 cut(s) 355, 785
ApaLI GTGCAC 1 cut(s) 725
ApeKI GCWGC 1 cut(s) 744
ApoI RAATTY 3 cut(s) 344, 652, 665
AsiGI ACCGGT 1 cut(s) 703
AspS9I GGNCC 2 cut(s) 356, 785
AsuC2I CCSGG 2 cut(s) 750, 751
AsuHPI GGTGA 3 cut(s) 121, 166, 703
AvaI CYCGRG 1 cut(s) 749
BaeGI GKGCMC 2 cut(s) 729, 762
BanII GRGCYC 2 cut(s) 584, 596
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 2 cut(s) 584, 729
BbvCI CCTCAGC 1 cut(s) 194
BbvI GCAGC 1 cut(s) 756
BccI CCATC 2 cut(s) 620, 812
BceAI ACGGC 2 cut(s) 235, 841
BciT130I CCWGG 1 cut(s) 783
BclI TGATCA 1 cut(s) 841
BcnI CCSGG 2 cut(s) 750, 751
BcoDI GTCTC 1 cut(s) 417
BcuI ACTAGT 1 cut(s) 872
BfaI CTAG 1 cut(s) 873
BfuAI ACCTGC 1 cut(s) 679
BisI GCNGC 5 cut(s) 352, 355, 745, 788, 829
BlsI GCNGC 5 cut(s) 353, 356, 746, 789, 830
Bme1390I CCNGG 3 cut(s) 750, 751, 783
BmeT110I CYCGRG 1 cut(s) 749
BmgBI CACGTC 1 cut(s) 724
BmgT120I GGNCC 2 cut(s) 356, 785
BmrFI CCNGG 3 cut(s) 750, 751, 783
BmrI ACTGGG 1 cut(s) 765
BmuI ACTGGG 1 cut(s) 765
BoxI GACNNNNGTC 1 cut(s) 116
BpiI GAAGAC 1 cut(s) 81
Bpu10I CCTNAGC 1 cut(s) 194
BpuMI CCSGG 2 cut(s) 750, 751
BsaAI YACGTR 1 cut(s) 237
BsaJI CCNNGG 4 cut(s) 257, 748, 749, 782
BsaWI WCCGGW 1 cut(s) 703
BsaXI ACNNNNNCTCC 2 cut(s) 395, 425
Bsc4I CCNNNNNNNGG 4 cut(s) 475, 612, 755, 756
Bse118I RCCGGY 1 cut(s) 703
Bse1I ACTGG 1 cut(s) 760
BseBI CCWGG 1 cut(s) 783
BseDI CCNNGG 4 cut(s) 257, 748, 749, 782
BseLI CCNNNNNNNGG 4 cut(s) 475, 612, 755, 756
BseMII CTCAG 2 cut(s) 208, 881
BseNI ACTGG 1 cut(s) 760
BseSI GKGCMC 2 cut(s) 729, 762
BseXI GCAGC 1 cut(s) 756
BshFI GGCC 2 cut(s) 357, 787
BshTI ACCGGT 1 cut(s) 703
BsiHKAI GWGCWC 2 cut(s) 584, 729
BsiHKCI CYCGRG 1 cut(s) 749
BsiSI CCGG 3 cut(s) 704, 750, 791
BslFI GGGAC 2 cut(s) 734, 766
BslI CCNNNNNNNGG 4 cut(s) 475, 612, 755, 756
BsmAI GTCTC 1 cut(s) 417
BsmFI GGGAC 2 cut(s) 734, 766
BsmI GAATGC 1 cut(s) 198
BsnI GGCC 2 cut(s) 357, 787
BsoBI CYCGRG 1 cut(s) 749
Bsp1286I GDGCHC 4 cut(s) 584, 596, 729, 762
Bsp143I GATC 8 cut(s) 17, 66, 159, 328, 397, 615, 805, 841
Bsp19I CCATGG 1 cut(s) 257
BspACI CCGC 7 cut(s) 96, 148, 222, 351, 354, 788, 828
BspANI GGCC 2 cut(s) 357, 787
BspCNI CTCAG 2 cut(s) 207, 880
BspHI TCATGA 1 cut(s) 180
BspMI ACCTGC 1 cut(s) 679
BspPI GGATC 2 cut(s) 336, 623
BsrFI RCCGGY 1 cut(s) 703
BsrI ACTGG 1 cut(s) 760
BssAI RCCGGY 1 cut(s) 703
BssECI CCNNGG 4 cut(s) 257, 748, 749, 782
BssMI GATC 8 cut(s) 17, 66, 159, 328, 397, 615, 805, 841
BssT1I CCWWGG 1 cut(s) 257
Bst2UI CCWGG 1 cut(s) 783
Bst4CI ACNGT 2 cut(s) 109, 839
BstAPI GCANNNNNTGC 2 cut(s) 134, 706
BstBAI YACGTR 1 cut(s) 237
BstDEI CTNAG 3 cut(s) 194, 214, 867
BstDSI CCRYGG 1 cut(s) 257
BstENI CCTNNNNNAGG 1 cut(s) 473
BstKTI GATC 8 cut(s) 20, 69, 162, 331, 400, 618, 808, 844
BstMAI GTCTC 1 cut(s) 417
BstMBI GATC 8 cut(s) 17, 66, 159, 328, 397, 615, 805, 841
BstMWI GCNNNNNNNGC 6 cut(s) 134, 219, 706, 733, 787, 828
BstNI CCWGG 1 cut(s) 783
BstNSI RCATGY 3 cut(s) 117, 144, 700
BstPAI GACNNNNGTC 1 cut(s) 116
BstSCI CCNGG 3 cut(s) 748, 749, 781
BstSLI GKGCMC 2 cut(s) 729, 762
BstV1I GCAGC 1 cut(s) 756
BstV2I GAAGAC 1 cut(s) 81
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
BsuRI GGCC 2 cut(s) 357, 787
BtgI CCRYGG 1 cut(s) 257
BtrI CACGTC 1 cut(s) 724
BtsI GCAGTG 1 cut(s) 665
BtsIMutI CAGTG 2 cut(s) 665, 844
BveI ACCTGC 1 cut(s) 679
CciI TCATGA 1 cut(s) 180
Cfr10I RCCGGY 1 cut(s) 703
Cfr13I GGNCC 2 cut(s) 356, 785
Cfr9I CCCGGG 1 cut(s) 749
CseI GACGC 1 cut(s) 106
CspAI ACCGGT 1 cut(s) 703
DdeI CTNAG 3 cut(s) 194, 214, 867
DpnI GATC 8 cut(s) 19, 68, 161, 330, 399, 617, 807, 843
DpnII GATC 8 cut(s) 17, 66, 159, 328, 397, 615, 805, 841
EciI GGCGGA 1 cut(s) 163
Ecl136II GAGCTC 1 cut(s) 582
Eco130I CCWWGG 1 cut(s) 257
Eco24I GRGCYC 2 cut(s) 584, 596
Eco53kI GAGCTC 1 cut(s) 582
Eco88I CYCGRG 1 cut(s) 749
EcoICRI GAGCTC 1 cut(s) 582
EcoNI CCTNNNNNAGG 1 cut(s) 473
EcoRII CCWGG 1 cut(s) 781
EcoT14I CCWWGG 1 cut(s) 257
EcoT22I ATGCAT 1 cut(s) 916
EcoT38I GRGCYC 2 cut(s) 584, 596
ErhI CCWWGG 1 cut(s) 257
FaqI GGGAC 2 cut(s) 734, 766
FauNDI CATATG 1 cut(s) 164
FbaI TGATCA 1 cut(s) 841
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 5 cut(s) 352, 355, 745, 788, 829
FriOI GRGCYC 2 cut(s) 584, 596
Fsp4HI GCNGC 5 cut(s) 352, 355, 745, 788, 829
FspBI CTAG 1 cut(s) 873
GluI GCNGC 5 cut(s) 352, 355, 745, 788, 829
HaeIII GGCC 2 cut(s) 357, 787
HapII CCGG 3 cut(s) 704, 750, 791
HgaI GACGC 1 cut(s) 106
HinfI GANTC 5 cut(s) 13, 307, 640, 676, 794
HpaII CCGG 3 cut(s) 704, 750, 791
HphI GGTGA 3 cut(s) 121, 166, 703
Hpy166II GTNNAC 3 cut(s) 121, 694, 727
Hpy188I TCNGA 7 cut(s) 204, 373, 671, 681, 799, 870, 892
Hpy188III TCNNGA 5 cut(s) 181, 401, 459, 649, 809
Hpy8I GTNNAC 3 cut(s) 121, 694, 727
HpyAV CCTTC 2 cut(s) 145, 296
HpyCH4III ACNGT 2 cut(s) 109, 839
HpyCH4IV ACGT 3 cut(s) 236, 504, 723
HpyF10VI GCNNNNNNNGC 6 cut(s) 134, 219, 706, 733, 787, 828
HpyF3I CTNAG 3 cut(s) 194, 214, 867
HpySE526I ACGT 3 cut(s) 236, 504, 723
Ksp22I TGATCA 1 cut(s) 841
Kzo9I GATC 8 cut(s) 17, 66, 159, 328, 397, 615, 805, 841
LmnI GCTCC 1 cut(s) 270
Lsp1109I GCAGC 1 cut(s) 756
MaeI CTAG 1 cut(s) 873
MaeII ACGT 3 cut(s) 236, 504, 723
MaeIII GTNAC 4 cut(s) 82, 109, 154, 875
MalI GATC 8 cut(s) 19, 68, 161, 330, 399, 617, 807, 843
MboI GATC 8 cut(s) 17, 66, 159, 328, 397, 615, 805, 841
MboII GAAGA 6 cut(s) 76, 86, 323, 442, 879, 899
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 4 cut(s) 584, 596, 729, 762
MluCI AATT 3 cut(s) 344, 652, 665
MlyI GAGTC 1 cut(s) 22
MnlI CCTC 8 cut(s) 20, 57, 188, 203, 289, 310, 469, 665
Mph1103I ATGCAT 1 cut(s) 916
MslI CAYNNNNRTG 2 cut(s) 533, 572
MspI CCGG 3 cut(s) 704, 750, 791
MspR9I CCNGG 3 cut(s) 750, 751, 783
Mva1269I GAATGC 1 cut(s) 198
MvaI CCWGG 1 cut(s) 783
MwoI GCNNNNNNNGC 6 cut(s) 134, 219, 706, 733, 787, 828
NciI CCSGG 2 cut(s) 750, 751
NcoI CCATGG 1 cut(s) 257
NdeI CATATG 1 cut(s) 164
NdeII GATC 8 cut(s) 17, 66, 159, 328, 397, 615, 805, 841
NmuCI GTSAC 3 cut(s) 82, 109, 154
NsiI ATGCAT 1 cut(s) 916
NspI RCATGY 3 cut(s) 117, 144, 700
OliI CACNNNNGTG 1 cut(s) 533
PagI TCATGA 1 cut(s) 180
PaqCI CACCTGC 1 cut(s) 679
PciI ACATGT 1 cut(s) 140
PcsI WCGNNNNNNNCGW 1 cut(s) 22
PctI GAATGC 1 cut(s) 198
PfeI GAWTC 4 cut(s) 307, 640, 676, 794
PflFI GACNNNGTC 1 cut(s) 569
PinAI ACCGGT 1 cut(s) 703
PkrI GCNGC 5 cut(s) 353, 356, 746, 789, 830
PleI GAGTC 1 cut(s) 21
PpsI GAGTC 1 cut(s) 21
Ppu21I YACGTR 1 cut(s) 237
PscI ACATGT 1 cut(s) 140
PshAI GACNNNNGTC 1 cut(s) 116
Psp124BI GAGCTC 1 cut(s) 584
Psp6I CCWGG 1 cut(s) 781
PspGI CCWGG 1 cut(s) 781
PspPI GGNCC 2 cut(s) 356, 785
PsuI RGATCY 1 cut(s) 328
PsyI GACNNNGTC 1 cut(s) 569
RseI CAYNNNNRTG 2 cut(s) 533, 572
SacI GAGCTC 1 cut(s) 584
SatI GCNGC 5 cut(s) 352, 355, 745, 788, 829
Sau3AI GATC 8 cut(s) 17, 66, 159, 328, 397, 615, 805, 841
Sau96I GGNCC 2 cut(s) 356, 785
SchI GAGTC 1 cut(s) 22
ScrFI CCNGG 3 cut(s) 750, 751, 783
SduI GDGCHC 4 cut(s) 584, 596, 729, 762
SmaI CCCGGG 1 cut(s) 751
SmiMI CAYNNNNRTG 2 cut(s) 533, 572
SpeI ACTAGT 1 cut(s) 872
Sse9I AATT 3 cut(s) 344, 652, 665
SsiI CCGC 7 cut(s) 96, 148, 222, 351, 354, 788, 828
SspI AATATT 1 cut(s) 133
SspMI CTAG 1 cut(s) 873
SstI GAGCTC 1 cut(s) 584
StyD4I CCNGG 3 cut(s) 748, 749, 781
StyI CCWWGG 1 cut(s) 257
TaaI ACNGT 2 cut(s) 109, 839
TaiI ACGT 3 cut(s) 239, 507, 726
TaqI TCGA 5 cut(s) 16, 246, 294, 300, 596
TasI AATT 3 cut(s) 344, 652, 665
TauI GCSGC 4 cut(s) 354, 357, 790, 831
TfiI GAWTC 4 cut(s) 307, 640, 676, 794
TscAI CASTG 2 cut(s) 665, 844
TseFI GTSAC 3 cut(s) 82, 109, 154
TseI GCWGC 1 cut(s) 744
Tsp45I GTSAC 3 cut(s) 82, 109, 154
TspDTI ATGAA 5 cut(s) 197, 218, 443, 632, 899
TspMI CCCGGG 1 cut(s) 749
TspRI CASTG 2 cut(s) 665, 844
Tth111I GACNNNGTC 1 cut(s) 569
VneI GTGCAC 1 cut(s) 725
XagI CCTNNNNNAGG 1 cut(s) 473
XapI RAATTY 3 cut(s) 344, 652, 665
XceI RCATGY 3 cut(s) 117, 144, 700
XcmI CCANNNNNNNNNTGG 1 cut(s) 264
XmaI CCCGGG 1 cut(s) 749
XmiI GTMKAC 1 cut(s) 120
XspI CTAG 1 cut(s) 873
Zsp2I ATGCAT 1 cut(s) 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.