MD17G1122500.v1.1

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0011855
Physical Location & Seq
Forward (+)
10636915 .. 10640232
3318 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1122500.v1.1.491

Sequence Viewer

Length: 1632 bp
ATGGATTCTGATAACCATTACAACAGCAACAACAACGACAACAATAGCAGGAACAATGATTCAGCCACAATAAAAATAAATTCCCATGCTGAGATGGGATCAACACAGCCCAAATCAGTGTTTGAGTTTGAGGATCAGAGCCTAGTTGGGGGCGAGAAGCATTCATTGGACGAGGCTCCCCTCAGGGCTCTATATTCTGGTGGTGCAAATGACTGTTGTCCCCATGTCTTGAAGAATAGTGTTGTCGATAGCATCAGGATTGTAGTTTTCTCTGCCAAAATTAATTTGCTCATTCCTTTTGGCCCTTTAGCAATCGTAGCAATCGTGGTTGATAAATTAACCGGCCATCATGTCTTTCTTTTAAGCTTGTTGGGAATCATACATTTGGCGGAGCGATTAGTCTATGCCACAGAGCAGCTGGCTTGCTATACTGGACCAACAGTTGGAGGTGTTTTGAATGCCACCTTTGGAAACGCAACAGAATTGATAATATCAATTTACGCTCTGAAAAGAGGCATGATTCGTGTTGTTCAACAATCCTTAGGCTCAATCTTATCGAACGTGTTGCTCGTGCTTGGATGCGCATTCTTTGCTGGTGGACTTGTGCATTCACAGAGGGAGCAAGTGTTCAACAAGGCCATATCCAAAGGCACCGAGAATCCCAAAGATCCCAAGAGGTTAAGTTCATCTACATTTGCAAAGATGCAGTCTTTGAGTCGATCAATACGAGGTCAATCATTACATGAGCCTCCACCATTTGCAGAGAAGATCACAGAAGACCCTGTGACAACGAAAACCGCACAAAGCACGGTGACTTGCGTCTACCTTGCAAATATTGCGACATGTTGGCGGAAGGTGACGATCATATGGTGTAAGAACCTCATGAACCATTCCCTCAGCATTCGGATTGATAGCTTAGACGGCGGTTTTCATTATACGTGCAAAATCGACCTAAAGCCATGGAATTTTTGGAGCAAAAAAGGGTATAAGTCATTCGAGGTCGAAGGGAATCAAGGGGAGGTTTGTTGGGATCTTCATTCTGCAAAATTTGGCGGCGGCCCAGAACCTTGTTCTAACTTTTATGTTGCTCTTGTTTGCGATGAGGAGATTGTGTTGTTATTGGGAGACATGAAGAAAAAGGCATACAAGAGAACAAAATCCAGACCAGCCCTTGTGGAGGCACTTTTATACCACAAAAAAGAAAACGTGTTTGCCAAGAAAAGTTTTGCCACAAGAGTGAAGTTTGATGAGAATATAAAAGAGCATGACATTGTCGTGGAGAGCTCAACATCGGGCTCCATAGACCCCGAAATGTGGATCAGCATAGATGGGTTTGTGTTGATTCATGTCAAGAATCTGCAGTGGAAATTCAGAGGGAATCAGACCGTAATGGTGAACATGCAACCGGTTCAAGTTTTCTGGGACGTGCACGATTGGCTTTTCAGCAGCCCCGGGACTGGGCACGGGCTGTTTATTTTCAAGCCAGGGCCGCCGGAATCAGAGAGCGATCACGACAGAGATGGCAACGGCGGCATAGACAGTGATCATAGCAGTCCTTATTATTCAACTCAGACTAGTAACTCAACATCTTCTGACTTCTGCCTGTTTCTTCATGCATATAAGATTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

544

Amino Acids

60.25

Weight (kDa)

6.7

Isoelectric Point (pI)

38.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 118 - 207 8.9e-14 Sodium/calcium exchanger protein
DUF868 PF05910 267 - 541 1.2e-105 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011855)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04220 AT4G12690 AT4G12690 AT5G48270
fragaria_vesca FvH4_6g40240
malus_domestica MD09G1133700.v1.1 MD17G1121100.v1.1 MD17G1122500.v1.1
prunus_persica Prupe.3G192800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0155261
rosa_laevigata RLG00000020831
rosa_multiflora Rmu_sc0012066.1_g000008
rosa_roxburghii Rroxscaffold_2G00093820
rosa_rugosa Rorug02G0446400
rosa_samantha Rh2AG512900 Rh2BG523500 Rh2CG497900 Rh2DG533200
rosa_wichuraiana Rw2G042140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 583
AccB1I GGYRCC 1 cut(s) 650
AccB7I CCANNNNNTGG 1 cut(s) 443
AccI GTMKAC 1 cut(s) 822
AciI CCGC 8 cut(s) 389, 798, 850, 924, 1053, 1056, 1490, 1530
AclWI GGATC 5 cut(s) 106, 141, 662, 1038, 1325
AcoI YGGCCR 1 cut(s) 343
AcsI RAATTY 4 cut(s) 79, 964, 1046, 1367
AfiI CCNNNNNNNGG 6 cut(s) 148, 443, 1177, 1314, 1457, 1458
AflIII ACRYGT 3 cut(s) 561, 842, 1206
AgeI ACCGGT 1 cut(s) 1405
AgsI TTSAA 7 cut(s) 232, 457, 533, 631, 1412, 1480, 1566
AhlI ACTAGT 1 cut(s) 1574
AjiI CACGTC 1 cut(s) 1426
AjnI CCWGG 1 cut(s) 1483
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
AleI CACNNNNGTG 1 cut(s) 1235
AloI GAACNNNNNNTCC 2 cut(s) 611, 643
AluBI AGCT 4 cut(s) 366, 418, 915, 1284
AluI AGCT 4 cut(s) 366, 418, 915, 1284
Alw21I GWGCWC 2 cut(s) 1286, 1431
Alw26I GTCTC 1 cut(s) 1119
Alw44I GTGCAC 1 cut(s) 1427
AlwI GGATC 5 cut(s) 106, 141, 662, 1038, 1325
Ama87I CYCGRG 1 cut(s) 1451
AoxI GGCC 5 cut(s) 301, 343, 636, 1057, 1487
ApaLI GTGCAC 1 cut(s) 1427
ApeKI GCWGC 2 cut(s) 415, 1446
ApoI RAATTY 4 cut(s) 79, 964, 1046, 1367
ArsI GACNNNNNNTTYG 2 cut(s) 692, 724
AseI ATTAAT 1 cut(s) 282
AsiGI ACCGGT 1 cut(s) 1405
AspLEI GCGC 1 cut(s) 584
AspS9I GGNCC 4 cut(s) 302, 434, 1058, 1487
AsuC2I CCSGG 2 cut(s) 1452, 1453
AsuHPI GGTGA 3 cut(s) 823, 868, 1405
AvaI CYCGRG 1 cut(s) 1451
AvaII GGWCC 1 cut(s) 434
AxyI CCTNAGG 2 cut(s) 182, 541
BaeGI GKGCMC 2 cut(s) 1431, 1464
BanI GGYRCC 1 cut(s) 650
BanII GRGCYC 3 cut(s) 190, 1286, 1298
BauI CACGAG 1 cut(s) 569
BbsI GAAGAC 1 cut(s) 783
Bbv12I GWGCWC 2 cut(s) 1286, 1431
BbvCI CCTCAGC 1 cut(s) 896
BbvI GCAGC 2 cut(s) 427, 1458
BccI CCATC 4 cut(s) 88, 354, 1322, 1514
BceAI ACGGC 2 cut(s) 937, 1543
BcgI CGANNNNNNTGC 2 cut(s) 547, 581
BciT130I CCWGG 1 cut(s) 1485
BclI TGATCA 1 cut(s) 1543
BcnI CCSGG 2 cut(s) 1452, 1453
BcoDI GTCTC 1 cut(s) 1119
BcuI ACTAGT 1 cut(s) 1574
BfaI CTAG 2 cut(s) 143, 1575
BfmI CTRYAG 1 cut(s) 1358
BisI GCNGC 6 cut(s) 416, 1054, 1057, 1447, 1490, 1531
BlsI GCNGC 6 cut(s) 417, 1055, 1058, 1448, 1491, 1532
Bme1390I CCNGG 3 cut(s) 1452, 1453, 1485
Bme18I GGWCC 1 cut(s) 434
BmeT110I CYCGRG 1 cut(s) 1451
BmgBI CACGTC 1 cut(s) 1426
BmgT120I GGNCC 4 cut(s) 302, 434, 1058, 1487
BmiI GGNNCC 3 cut(s) 177, 652, 1297
BmrFI CCNGG 3 cut(s) 1452, 1453, 1485
BmrI ACTGGG 1 cut(s) 1467
BmsI GCATC 3 cut(s) 261, 569, 693
BmuI ACTGGG 1 cut(s) 1467
BoxI GACNNNNGTC 2 cut(s) 216, 818
BpiI GAAGAC 1 cut(s) 783
Bpu10I CCTNAGC 1 cut(s) 896
BpuMI CCSGG 2 cut(s) 1452, 1453
BsaAI YACGTR 1 cut(s) 939
BsaJI CCNNGG 4 cut(s) 959, 1450, 1451, 1484
BsaWI WCCGGW 1 cut(s) 1405
BsaXI ACNNNNNCTCC 4 cut(s) 611, 641, 1097, 1127
Bsc4I CCNNNNNNNGG 6 cut(s) 148, 443, 1177, 1314, 1457, 1458
Bse118I RCCGGY 2 cut(s) 341, 1405
Bse1I ACTGG 2 cut(s) 436, 1462
Bse21I CCTNAGG 2 cut(s) 182, 541
BseBI CCWGG 1 cut(s) 1485
BseDI CCNNGG 4 cut(s) 959, 1450, 1451, 1484
BseGI GGATG 1 cut(s) 584
BseLI CCNNNNNNNGG 6 cut(s) 148, 443, 1177, 1314, 1457, 1458
BseMII CTCAG 4 cut(s) 81, 196, 910, 1583
BseNI ACTGG 2 cut(s) 436, 1462
BseRI GAGGAG 1 cut(s) 1118
BseSI GKGCMC 2 cut(s) 1431, 1464
BseXI GCAGC 2 cut(s) 427, 1458
BshFI GGCC 5 cut(s) 303, 345, 638, 1059, 1489
BshNI GGYRCC 1 cut(s) 650
BshTI ACCGGT 1 cut(s) 1405
BsiHKAI GWGCWC 2 cut(s) 1286, 1431
BsiHKCI CYCGRG 1 cut(s) 1451
BsiSI CCGG 4 cut(s) 342, 1406, 1452, 1493
BslFI GGGAC 3 cut(s) 204, 1436, 1468
BslI CCNNNNNNNGG 6 cut(s) 148, 443, 1177, 1314, 1457, 1458
BsmAI GTCTC 1 cut(s) 1119
BsmFI GGGAC 3 cut(s) 204, 1436, 1468
BsmI GAATGC 5 cut(s) 160, 463, 584, 607, 900
BsnI GGCC 5 cut(s) 303, 345, 638, 1059, 1489
BsoBI CYCGRG 1 cut(s) 1451
Bsp1286I GDGCHC 5 cut(s) 190, 1286, 1298, 1431, 1464
Bsp19I CCATGG 1 cut(s) 959
BspACI CCGC 8 cut(s) 389, 798, 850, 924, 1053, 1056, 1490, 1530
BspANI GGCC 5 cut(s) 303, 345, 638, 1059, 1489
BspCNI CTCAG 4 cut(s) 82, 195, 909, 1582
BspHI TCATGA 1 cut(s) 882
BspLI GGNNCC 3 cut(s) 177, 652, 1297
BspMAI CTGCAG 1 cut(s) 1362
BspPI GGATC 5 cut(s) 106, 141, 662, 1038, 1325
BspT107I GGYRCC 1 cut(s) 650
BsrFI RCCGGY 2 cut(s) 341, 1405
BsrI ACTGG 2 cut(s) 436, 1462
BssAI RCCGGY 2 cut(s) 341, 1405
BssECI CCNNGG 4 cut(s) 959, 1450, 1451, 1484
BssSI CACGAG 1 cut(s) 569
BssT1I CCWWGG 1 cut(s) 959
Bst2BI CACGAG 1 cut(s) 569
Bst2UI CCWGG 1 cut(s) 1485
Bst4CI ACNGT 5 cut(s) 215, 442, 811, 1387, 1541
BstAPI GCANNNNNTGC 2 cut(s) 590, 836
BstBAI YACGTR 1 cut(s) 939
BstC8I GCNNGC 2 cut(s) 420, 424
BstDEI CTNAG 6 cut(s) 90, 182, 541, 896, 916, 1569
BstDSI CCRYGG 1 cut(s) 959
BstENI CCTNNNNNAGG 1 cut(s) 1175
BstF5I GGATG 1 cut(s) 584
BstHHI GCGC 1 cut(s) 584
BstMAI GTCTC 1 cut(s) 1119
BstMWI GCNNNNNNNGC 7 cut(s) 317, 590, 836, 921, 1435, 1489, 1530
BstNI CCWGG 1 cut(s) 1485
BstNSI RCATGY 2 cut(s) 846, 1402
BstPAI GACNNNNGTC 2 cut(s) 216, 818
BstSCI CCNGG 3 cut(s) 1450, 1451, 1483
BstSFI CTRYAG 1 cut(s) 1358
BstSLI GKGCMC 2 cut(s) 1431, 1464
BstV1I GCAGC 2 cut(s) 427, 1458
BstV2I GAAGAC 1 cut(s) 783
BstX2I RGATCY 2 cut(s) 667, 1030
BstYI RGATCY 2 cut(s) 667, 1030
Bsu36I CCTNAGG 2 cut(s) 182, 541
BsuRI GGCC 5 cut(s) 303, 345, 638, 1059, 1489
BtgI CCRYGG 1 cut(s) 959
BtgZI GCGATG 1 cut(s) 1113
BtrI CACGTC 1 cut(s) 1426
BtsCI GGATG 1 cut(s) 584
BtsI GCAGTG 1 cut(s) 1367
BtsIMutI CAGTG 3 cut(s) 123, 1367, 1546
Cac8I GCNNGC 2 cut(s) 420, 424
CciI TCATGA 1 cut(s) 882
CfoI GCGC 1 cut(s) 584
Cfr10I RCCGGY 2 cut(s) 341, 1405
Cfr13I GGNCC 4 cut(s) 302, 434, 1058, 1487
Cfr9I CCCGGG 1 cut(s) 1451
CseI GACGC 1 cut(s) 808
CspAI ACCGGT 1 cut(s) 1405
DdeI CTNAG 6 cut(s) 90, 182, 541, 896, 916, 1569
EaeI YGGCCR 1 cut(s) 343
EciI GGCGGA 2 cut(s) 404, 865
Ecl136II GAGCTC 1 cut(s) 1284
Eco130I CCWWGG 1 cut(s) 959
Eco24I GRGCYC 3 cut(s) 190, 1286, 1298
Eco47I GGWCC 1 cut(s) 434
Eco53kI GAGCTC 1 cut(s) 1284
Eco81I CCTNAGG 2 cut(s) 182, 541
Eco88I CYCGRG 1 cut(s) 1451
EcoICRI GAGCTC 1 cut(s) 1284
EcoNI CCTNNNNNAGG 1 cut(s) 1175
EcoRII CCWGG 1 cut(s) 1483
EcoT14I CCWWGG 1 cut(s) 959
EcoT22I ATGCAT 1 cut(s) 1618
EcoT38I GRGCYC 3 cut(s) 190, 1286, 1298
ErhI CCWWGG 1 cut(s) 959
FaqI GGGAC 3 cut(s) 204, 1436, 1468
FauNDI CATATG 1 cut(s) 866
FbaI TGATCA 1 cut(s) 1543
FblI GTMKAC 1 cut(s) 822
Fnu4HI GCNGC 6 cut(s) 416, 1054, 1057, 1447, 1490, 1531
FokI GGATG 1 cut(s) 591
FriOI GRGCYC 3 cut(s) 190, 1286, 1298
Fsp4HI GCNGC 6 cut(s) 416, 1054, 1057, 1447, 1490, 1531
FspAI RTGCGCAY 1 cut(s) 583
FspBI CTAG 2 cut(s) 143, 1575
FspI TGCGCA 1 cut(s) 583
GlaI GCGC 1 cut(s) 583
GluI GCNGC 6 cut(s) 416, 1054, 1057, 1447, 1490, 1531
HaeIII GGCC 5 cut(s) 303, 345, 638, 1059, 1489
HapII CCGG 4 cut(s) 342, 1406, 1452, 1493
HgaI GACGC 1 cut(s) 808
HhaI GCGC 1 cut(s) 584
Hin6I GCGC 1 cut(s) 582
HinP1I GCGC 1 cut(s) 582
HindIII AAGCTT 1 cut(s) 364
HpaII CCGG 4 cut(s) 342, 1406, 1452, 1493
HphI GGTGA 3 cut(s) 823, 868, 1405
Hpy166II GTNNAC 4 cut(s) 599, 823, 1396, 1429
Hpy188I TCNGA 9 cut(s) 10, 138, 507, 906, 1373, 1383, 1501, 1572, 1594
Hpy188III TCNNGA 6 cut(s) 229, 256, 883, 1161, 1351, 1511
Hpy8I GTNNAC 4 cut(s) 599, 823, 1396, 1429
HpyAV CCTTC 2 cut(s) 847, 998
HpyCH4III ACNGT 5 cut(s) 215, 442, 811, 1387, 1541
HpyCH4IV ACGT 4 cut(s) 561, 938, 1206, 1425
HpyF10VI GCNNNNNNNGC 7 cut(s) 317, 590, 836, 921, 1435, 1489, 1530
HpyF3I CTNAG 6 cut(s) 90, 182, 541, 896, 916, 1569
HpySE526I ACGT 4 cut(s) 561, 938, 1206, 1425
HspAI GCGC 1 cut(s) 582
Ksp22I TGATCA 1 cut(s) 1543
LmnI GCTCC 5 cut(s) 181, 391, 619, 972, 1301
Lsp1109I GCAGC 2 cut(s) 427, 1458
LweI GCATC 3 cut(s) 261, 569, 693
MaeI CTAG 2 cut(s) 143, 1575
MaeII ACGT 4 cut(s) 561, 938, 1206, 1425
MaeIII GTNAC 4 cut(s) 784, 811, 856, 1577
MboII GAAGA 7 cut(s) 244, 778, 788, 1025, 1144, 1581, 1601
MflI RGATCY 2 cut(s) 667, 1030
MhlI GDGCHC 5 cut(s) 190, 1286, 1298, 1431, 1464
MluCI AATT 9 cut(s) 79, 279, 283, 335, 482, 495, 964, 1046, 1367
MlyI GAGTC 1 cut(s) 724
MmeI TCCRAC 1 cut(s) 424
Mph1103I ATGCAT 1 cut(s) 1618
MseI TTAA 4 cut(s) 282, 338, 362, 680
MslI CAYNNNNRTG 2 cut(s) 1235, 1274
MspA1I CMGCKG 1 cut(s) 418
MspI CCGG 4 cut(s) 342, 1406, 1452, 1493
MspR9I CCNGG 3 cut(s) 1452, 1453, 1485
Mva1269I GAATGC 5 cut(s) 160, 463, 584, 607, 900
MvaI CCWGG 1 cut(s) 1485
MwoI GCNNNNNNNGC 7 cut(s) 317, 590, 836, 921, 1435, 1489, 1530
NciI CCSGG 2 cut(s) 1452, 1453
NcoI CCATGG 1 cut(s) 959
NdeI CATATG 1 cut(s) 866
NlaIV GGNNCC 3 cut(s) 177, 652, 1297
NmuCI GTSAC 3 cut(s) 784, 811, 856
NsbI TGCGCA 1 cut(s) 583
NsiI ATGCAT 1 cut(s) 1618
NspI RCATGY 2 cut(s) 846, 1402
OliI CACNNNNGTG 1 cut(s) 1235
PagI TCATGA 1 cut(s) 882
PciI ACATGT 1 cut(s) 842
PcsI WCGNNNNNNNCGW 3 cut(s) 321, 567, 724
PctI GAATGC 5 cut(s) 160, 463, 584, 607, 900
PflFI GACNNNGTC 1 cut(s) 1271
PflMI CCANNNNNTGG 1 cut(s) 443
PinAI ACCGGT 1 cut(s) 1405
PkrI GCNGC 6 cut(s) 417, 1055, 1058, 1448, 1491, 1532
PleI GAGTC 1 cut(s) 723
PpsI GAGTC 1 cut(s) 723
Ppu21I YACGTR 1 cut(s) 939
PscI ACATGT 1 cut(s) 842
PshAI GACNNNNGTC 2 cut(s) 216, 818
PshBI ATTAAT 1 cut(s) 282
Psp124BI GAGCTC 1 cut(s) 1286
Psp6I CCWGG 1 cut(s) 1483
PspGI CCWGG 1 cut(s) 1483
PspN4I GGNNCC 3 cut(s) 177, 652, 1297
PspPI GGNCC 4 cut(s) 302, 434, 1058, 1487
PstI CTGCAG 1 cut(s) 1362
PsuI RGATCY 2 cut(s) 667, 1030
PsyI GACNNNGTC 1 cut(s) 1271
PvuII CAGCTG 1 cut(s) 418
RseI CAYNNNNRTG 2 cut(s) 1235, 1274
SacI GAGCTC 1 cut(s) 1286
SaqAI TTAA 4 cut(s) 282, 338, 362, 680
SatI GCNGC 6 cut(s) 416, 1054, 1057, 1447, 1490, 1531
Sau96I GGNCC 4 cut(s) 302, 434, 1058, 1487
SchI GAGTC 1 cut(s) 724
ScrFI CCNGG 3 cut(s) 1452, 1453, 1485
SduI GDGCHC 5 cut(s) 190, 1286, 1298, 1431, 1464
SfaNI GCATC 3 cut(s) 261, 569, 693
SfcI CTRYAG 1 cut(s) 1358
SinI GGWCC 1 cut(s) 434
SmaI CCCGGG 1 cut(s) 1453
SmiMI CAYNNNNRTG 2 cut(s) 1235, 1274
SpeI ACTAGT 1 cut(s) 1574
Sse9I AATT 9 cut(s) 79, 279, 283, 335, 482, 495, 964, 1046, 1367
SsiI CCGC 8 cut(s) 389, 798, 850, 924, 1053, 1056, 1490, 1530
SspI AATATT 1 cut(s) 835
SspMI CTAG 2 cut(s) 143, 1575
SstI GAGCTC 1 cut(s) 1286
StyD4I CCNGG 3 cut(s) 1450, 1451, 1483
StyI CCWWGG 1 cut(s) 959
TaaI ACNGT 5 cut(s) 215, 442, 811, 1387, 1541
TaiI ACGT 4 cut(s) 564, 941, 1209, 1428
TaqI TCGA 6 cut(s) 246, 557, 718, 948, 996, 1002
TasI AATT 9 cut(s) 79, 279, 283, 335, 482, 495, 964, 1046, 1367
TauI GCSGC 4 cut(s) 1056, 1059, 1492, 1533
Tru1I TTAA 4 cut(s) 282, 338, 362, 680
Tru9I TTAA 4 cut(s) 282, 338, 362, 680
TscAI CASTG 3 cut(s) 123, 1367, 1546
TseFI GTSAC 3 cut(s) 784, 811, 856
TseI GCWGC 2 cut(s) 415, 1446
Tsp45I GTSAC 3 cut(s) 784, 811, 856
TspDTI ATGAA 8 cut(s) 153, 675, 899, 920, 1025, 1145, 1334, 1601
TspMI CCCGGG 1 cut(s) 1451
TspRI CASTG 3 cut(s) 123, 1367, 1546
Tth111I GACNNNGTC 1 cut(s) 1271
Van91I CCANNNNNTGG 1 cut(s) 443
VneI GTGCAC 1 cut(s) 1427
VpaK11BI GGWCC 1 cut(s) 434
VspI ATTAAT 1 cut(s) 282
XagI CCTNNNNNAGG 1 cut(s) 1175
XapI RAATTY 4 cut(s) 79, 964, 1046, 1367
XceI RCATGY 2 cut(s) 846, 1402
XcmI CCANNNNNNNNNTGG 2 cut(s) 415, 966
XmaI CCCGGG 1 cut(s) 1451
XmiI GTMKAC 1 cut(s) 822
XspI CTAG 2 cut(s) 143, 1575
Zsp2I ATGCAT 1 cut(s) 1618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.