Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0007704

Physical Location & Seq

Genomic Coordinates Forward (+)

7353773 .. 7361256

Gene Structure 7484 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G16950.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2688 bp

ATGGCGGCGACGGCGGTGGTCTGGCAACCCCGAGACGATGGTCTCGCTGAGATCTGTTCTCTTCTCGAACAACAGATTTCTCCTTCCTCCGTCGTTGACAAGTCTCAGATTTGGAAGCAGCTTCAACACTTCTCTCAGTTTCCTGATTTCAACAACTATCTCGTCTTCATCCTCGTTCGCGCTGAGGGAAAGTCTGTTGAGGTTCGTCAAGCTGCTGGATTGCTTTTGAAAAATAATTTGAGGGGTGCTTATCCATCCATGACTCAAGAAAACCAAAAATATATCAAGTCAGAGTTACTGCCTTGTCTGGGAGCAGCGGACAGGAATATACGGACTACAGTTGGAACTATCATCAGTGTTATTGTTAATATTGAGGGAGTTTCTGGATGGCATGAGCTTTTGCCAGCTCTTGTTACTTGTTTAGACAGTAATGACCTTAATCACATGGATGGTGCTATGGATGCATTGTCAAAGATTTGTGAGGATATTCCGCATGTCTTGGATACAGAAGTGCCTGGCTTAGCAGAACGCCCTATCAACATTTTCCTGCCTAGGCTATTACAGTTTTTTCAGTCACCCCATGCTTCACTGAGGAAGCTTGCCCTGGGTTCTGTGAACCAGTATATCATTATAATGCCTGCTGTAATTTGGCAGGCTTTGTACAATTCATTGGATAAGTACCTTCAGGGGTTGTTTGTCCTAGCCAATGATCCTGTACCAGAAGTCAGAAAATTGGTCTGCGCAGCATTTGTGCATCTCACGGAAGTACTCCCCTCATCTATAGAGCCGCATCTCAGGAATGTCATGGAATACATGTTACAAGTTAATAGAGACCCTGACGAAGAAGTGTCGCTCGAAGCATGTGAATTTTGGTCTGCATACTGTGATGCTCAGCTACCTCCAGAGAACTTGAAAGAGTTTCTGCCACGCCTAATTCCAGTGTTGCTCGAAAACATGGCTTATGCAGATGACGACGAGTCACTTTTGGATGCAGAGGAAGATGAGTCTCAACCAGACAGAGATCAGGATTTAAAACCTCGTTTTCACACATCAAGACTTCATGGATCAGAAGATTTTGATGATGATGATGATGACTCGTTTAATGTGTGGAATTTAAGAAAGTGTAGTGCAGCAGCTATTGACGTTCTCTCTAATGTTTTTGGAGATGAAATCCTTCCAGCACTCATGCCCCTAATTCAGAAAAATTTATCAGCTTCTGGTGATGAGGCCTGGAAACAAAGGGAAGCTGCTGTTTTGGCTCTCGGCGCTATTGCTGAAGGCTGCATGAATGGTCTTTACCCTCACTTGTCTGAGATCGTGGCATTTCTTCTTCCTCTTTTAGATGATAAATTCCCGCTCATACGGAGCATATCTTGCTGGACGCTTTCTCGATTTGGCAAGTATCTTATCCAGGAAAGTGGCAATCCGAAGGGTTATGAACAATTTGAGAAAGTTCTTATGGGTCTTCTCCGCAGACTCTTGGATACGAACAAGCGGGTTCAGGAGGCTGCCTGTTCAGCTTTTGCAACTGTTGAAGAGGATGCTGCTGAAGAGTTAGTGCCACACCTGGGTGTAATACTGCAGCATCTGATGTGCGCTTTTGGAAAGTATCAGAGACGGAATTTAAGAATTGTTTATGATGCTATTGGAACACTGGCAGATTCAGTTAGAGAAGAGTTGAACAAGCCTGCTTATCTTGAGATTCTGATGCCTCCACTGGTTGCAAAGTGGCAACAACTTTCAAATTCAGATAAAGATCTATTTCCATTGCTAGAGTGCTTTACATCCATTTCTCAGGCTTTAGGTGTAGGATTCGCTCCATTTGCTCAGCCTGTTTTCCAGAGATGCATGGATATCATCCAATTGCAGCAGCTGGCTAAGGTTAATCCTGCTTCTGCTGGGGCTCAGTATGACAGAGAATTTATTGTTTGTTCTCTTGATTTGCTTTCTGGACTTGCTGAAGGGCTTGGCAGTGGGATAGAGTCTCTGGTTCAACAAAGTAACCTAAGGGATCTGCTTCTCAATTGTTGCATTGATGAAGCTGCTGATGTCAGACAAAGTGCTTTTGCCTTGATGGGTGATCTAGCAAGAGTTTTCCCAGTCTATCTACAACCCCGCCTGCTTGATTTCCTTGAAATTGCAAGCCAGCAACTGAGTGCAAACCTTAATAGAGAAAACCTTTCTGTCGCAAATAATGCTTGTTGGGCCATTGGAGAATTAGCGGTCAAGGTTCGTCAAGAAGTCTCACCAATTGTGGCCAAAGTTGTCTCTTCCCTAGGCCTGATTCTTCAACATGGAGAGGGTGTCAATAAGGCACTAGTTGAGAACAGTGCAATAACCCTTGGGAGACTAGCATGGATTCGTCCAGACCTCGTTGCACCACACATGGACCACTTTATGAAGCCATGGTGCATGGCATTGTCAATGGTACGTGATGACATTGAGAAAGAAGATGCGTTTAGAGGATTATGTGCAGTGGTTAAAGTGAATCCATCAGGAGGTGTCAGCTCACTTGTTTTCATATGCCAAGCCATTGCGAGTTGGCATGAAATAAGAAGCGAGGATGTCCAGACCGAAGTCTCCCAAGTACTGAACGGTTATAAACATATGCTGGGAAACTCATGGGCGGAATGTTTATCTGCTTTGGATCCTCCTGTAAAGGAAAGGCTTGCGAGATATCAAGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000060 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005829 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0009892 GO:0009987 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016441 GO:0016458 GO:0017038 GO:0019222 GO:0022607 GO:0022613 GO:0022618 GO:0031047 GO:0031090 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0033036 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0034622 GO:0035194 GO:0035195 GO:0035280 GO:0040029 GO:0042277 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048519 GO:0050789 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0070727 GO:0070922 GO:0071702 GO:0071705 GO:0071826 GO:0071840 GO:0072594

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HEAT HEAT_EZ IBN_N Ribosomal_S17

Protein Analysis

895

Amino Acids

99.63

Weight (kDa)

4.81

Isoelectric Point (pI)

48.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IBN_N	PF03810	38 - 104	9.2e-15	Importin-beta N-terminal domain
HEAT_EZ	PF13513	411 - 465	4.4e-16	HEAT-like repeat
TPR_IMB1	PF25574	486 - 700	7.5e-09	Importin subunit beta-1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007704)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16950 AT2G16950
fragaria_vesca	FvH4_2g15650 FvH4_2g15650 FvH4_2g15650 FvH4_2g15650 FvH4_2g15650 FvH4_2g15650
malus_domestica	MD05G1125000.v1.1 MD10G1127800.v1.1
prunus_persica	Prupe.8G169000_v2.0.a1 Prupe.8G169000_v2.0.a1 Prupe.8G169000_v2.0.a1
pyrus_communis	pycom05g11910 pycom10g10950
rosa_chinensis	RchiOBHm_Chr6g0278851
rosa_laevigata	RLG00000013189
rosa_multiflora	Rmu_sc0004380.1_g000009 Rmu_sc0024310.1_g000003
rosa_roxburghii	Rroxscaffold_7G00189590
rosa_rugosa	Rorug06G0119700
rosa_samantha	Rh6AG228200 Rh6BG233800
rosa_wichuraiana	Rw6G020020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	632, 2601
Acc16I	TGCGCA	1 cut(s)	742
AccBSI	CCGCTC	1 cut(s)	1357
AccII	CGCG	1 cut(s)	180
AclWI	GGATC	5 cut(s)	704, 1072, 2019, 2642, 2655
AcoI	YGGCCR	1 cut(s)	2256
AcsI	RAATTY	7 cut(s)	866, 1111, 1204, 1349, 1621, 1744, 1919
AcuI	CTGAAG	4 cut(s)	668, 1296, 1569, 1980
AfaI	GTAC	6 cut(s)	662, 680, 717, 768, 2430, 2589
AfiI	CCNNNNNNNGG	3 cut(s)	308, 1568, 2498
AflIII	ACRYGT	1 cut(s)	813
AhdI	GACNNNNNGTC	1 cut(s)	976
AhlI	ACTAGT	1 cut(s)	2317
AjnI	CCWGG	5 cut(s)	514, 603, 1229, 1410, 1566
AleI	CACNNNNGTG	1 cut(s)	1569
AlwI	GGATC	5 cut(s)	704, 1072, 2019, 2642, 2655
AlwNI	CAGNNNCTG	4 cut(s)	1217, 1588, 1873, 2152
Ama87I	CYCGRG	1 cut(s)	30
AoxI	GGCC	4 cut(s)	1227, 2205, 2256, 2278
ApoI	RAATTY	7 cut(s)	866, 1111, 1204, 1349, 1621, 1744, 1919
Asp700I	GAANNNNTTC	1 cut(s)	1173
AspA2I	CCTAGG	2 cut(s)	551, 2275
AspLEI	GCGC	4 cut(s)	182, 743, 1268, 1598
AspS9I	GGNCC	2 cut(s)	2205, 2389
AsuHPI	GGTGA	4 cut(s)	567, 1232, 2090, 2238
AvaI	CYCGRG	1 cut(s)	30
AvaII	GGWCC	1 cut(s)	2389
AvrII	CCTAGG	2 cut(s)	551, 2275
AxyI	CCTNAGG	1 cut(s)	2006
BaeI	ACNNNNGTAYC	2 cut(s)	495, 528
BalI	TGGCCA	1 cut(s)	2258
BamHI	GGATCC	1 cut(s)	2647
BanII	GRGCYC	1 cut(s)	1906
BbsI	GAAGAC	2 cut(s)	157, 1457
BbvCI	CCTCAGC	1 cut(s)	183
BccI	CCATC	6 cut(s)	32, 262, 381, 443, 2068, 2500
BceAI	ACGGC	1 cut(s)	27
BciT130I	CCWGG	5 cut(s)	516, 605, 1231, 1412, 1568
BciVI	GTATCC	2 cut(s)	496, 1477
BcuI	ACTAGT	1 cut(s)	2317
BfaI	CTAG	7 cut(s)	552, 701, 1772, 2084, 2276, 2318, 2351
BfmI	CTRYAG	3 cut(s)	336, 780, 1580
BfoI	RGCGCY	1 cut(s)	1269
BfuI	GTATCC	2 cut(s)	496, 1477
BglII	AGATCT	2 cut(s)	51, 1756
BlnI	CCTAGG	2 cut(s)	551, 2275
BlpI	GCTNAGC	3 cut(s)	520, 891, 1827
BmcAI	AGTACT	2 cut(s)	768, 2589
Bme1390I	CCNGG	5 cut(s)	516, 605, 1231, 1412, 1568
Bme18I	GGWCC	1 cut(s)	2389
BmeRI	GACNNNNNGTC	1 cut(s)	976
BmeT110I	CYCGRG	1 cut(s)	30
BmgT120I	GGNCC	2 cut(s)	2205, 2389
BmiI	GGNNCC	1 cut(s)	2649
BmrFI	CCNGG	5 cut(s)	516, 605, 1231, 1412, 1568
BmrI	ACTGGG	1 cut(s)	2093
BmuI	ACTGGG	1 cut(s)	2093
BoxI	GACNNNNGTC	2 cut(s)	39, 2576
BpiI	GAAGAC	2 cut(s)	157, 1457
BpmI	CTGGAG	1 cut(s)	885
Bpu10I	CCTNAGC	2 cut(s)	183, 1878
Bpu1102I	GCTNAGC	3 cut(s)	520, 891, 1827
BpuEI	CTTGAG	2 cut(s)	249, 1718
BsaAI	YACGTR	1 cut(s)	2432
BsaBI	GATNNNNATC	1 cut(s)	1755
BsaI	GGTCTC	2 cut(s)	47, 825
BsaJI	CCNNGG	7 cut(s)	551, 603, 604, 1567, 2275, 2341, 2405
BsaXI	ACNNNNNCTCC	2 cut(s)	2289, 2319
Bsc4I	CCNNNNNNNGG	3 cut(s)	308, 1568, 2498
Bse1I	ACTGG	5 cut(s)	619, 938, 1659, 1722, 2099
Bse21I	CCTNAGG	1 cut(s)	2006
Bse3DI	GCAATG	2 cut(s)	1766, 2532
Bse8I	GATNNNNATC	1 cut(s)	1755
BseBI	CCWGG	5 cut(s)	516, 605, 1231, 1412, 1568
BseDI	CCNNGG	7 cut(s)	551, 603, 604, 1567, 2275, 2341, 2405
BseJI	GATNNNNATC	1 cut(s)	1755
BseLI	CCNNNNNNNGG	3 cut(s)	308, 1568, 2498
BseMI	GCAATG	2 cut(s)	1766, 2532
BseNI	ACTGG	5 cut(s)	619, 938, 1659, 1722, 2099
BseYI	CCCAGC	2 cut(s)	1898, 2611
BsgI	GTGCAG	2 cut(s)	1149, 2493
Bsh1236I	CGCG	1 cut(s)	180
BshFI	GGCC	4 cut(s)	1229, 2207, 2258, 2280
BsiHKCI	CYCGRG	1 cut(s)	30
BslI	CCNNNNNNNGG	3 cut(s)	308, 1568, 2498
BsmBI	CGTCTC	2 cut(s)	27, 1609
BsnI	GGCC	4 cut(s)	1229, 2207, 2258, 2280
Bso31I	GGTCTC	2 cut(s)	47, 825
BsoBI	CYCGRG	1 cut(s)	30
Bsp1286I	GDGCHC	1 cut(s)	1906
Bsp1407I	TGTACA	1 cut(s)	660
Bsp143I	GATC	9 cut(s)	51, 709, 1021, 1064, 1314, 1756, 2011, 2080, 2647
Bsp1720I	GCTNAGC	3 cut(s)	520, 891, 1827
Bsp19I	CCATGG	1 cut(s)	2405
BspANI	GGCC	4 cut(s)	1229, 2207, 2258, 2280
BspFNI	CGCG	1 cut(s)	180
BspLI	GGNNCC	1 cut(s)	2649
BspMAI	CTGCAG	1 cut(s)	1584
BspPI	GGATC	5 cut(s)	704, 1072, 2019, 2642, 2655
BspTNI	GGTCTC	2 cut(s)	47, 825
BsrBI	CCGCTC	1 cut(s)	1357
BsrDI	GCAATG	2 cut(s)	1766, 2532
BsrGI	TGTACA	1 cut(s)	660
BsrI	ACTGG	5 cut(s)	619, 938, 1659, 1722, 2099
BssECI	CCNNGG	7 cut(s)	551, 603, 604, 1567, 2275, 2341, 2405
BssMI	GATC	9 cut(s)	51, 709, 1021, 1064, 1314, 1756, 2011, 2080, 2647
BssT1I	CCWWGG	4 cut(s)	551, 2275, 2341, 2405
Bst2UI	CCWGG	5 cut(s)	516, 605, 1231, 1412, 1568
Bst4CI	ACNGT	7 cut(s)	340, 428, 564, 884, 1531, 2330, 2597
Bst6I	CTCTTC	5 cut(s)	66, 1530, 1545, 1668, 2275
BstAPI	GCANNNNNTGC	2 cut(s)	1374, 2195
BstAUI	TGTACA	1 cut(s)	660
BstBAI	YACGTR	1 cut(s)	2432
BstDSI	CCRYGG	1 cut(s)	2405
BstFNI	CGCG	1 cut(s)	180
BstH2I	RGCGCY	1 cut(s)	1269
BstHHI	GCGC	4 cut(s)	182, 743, 1268, 1598
BstKTI	GATC	9 cut(s)	54, 712, 1024, 1067, 1317, 1759, 2014, 2083, 2650
BstMBI	GATC	9 cut(s)	51, 709, 1021, 1064, 1314, 1756, 2011, 2080, 2647
BstMWI	GCNNNNNNNGC	9 cut(s)	11, 461, 1256, 1265, 1374, 1517, 1823, 2195, 2204
BstNI	CCWGG	5 cut(s)	516, 605, 1231, 1412, 1568
BstNSI	RCATGY	3 cut(s)	497, 817, 864
BstPAI	GACNNNNGTC	2 cut(s)	39, 2576
BstSCI	CCNGG	5 cut(s)	514, 603, 1229, 1410, 1566
BstSFI	CTRYAG	3 cut(s)	336, 780, 1580
BstUI	CGCG	1 cut(s)	180
BstV2I	GAAGAC	2 cut(s)	157, 1457
BstX2I	RGATCY	4 cut(s)	51, 1756, 2011, 2647
BstXI	CCANNNNNNTGG	1 cut(s)	1418
BstYI	RGATCY	4 cut(s)	51, 1756, 2011, 2647
Bsu36I	CCTNAGG	1 cut(s)	2006
BsuI	GTATCC	2 cut(s)	496, 1477
BsuRI	GGCC	4 cut(s)	1229, 2207, 2258, 2280
BtgI	CCRYGG	1 cut(s)	2405
BtsI	GCAGTG	2 cut(s)	1978, 2481
BtsIMutI	CAGTG	8 cut(s)	361, 587, 945, 1652, 1715, 1978, 2335, 2481
CaiI	CAGNNNCTG	4 cut(s)	1217, 1588, 1873, 2152
CfoI	GCGC	4 cut(s)	182, 743, 1268, 1598
Cfr13I	GGNCC	2 cut(s)	2205, 2389
CseI	GACGC	1 cut(s)	1390
Csp6I	GTAC	6 cut(s)	661, 679, 716, 767, 2429, 2588
CviQI	GTAC	6 cut(s)	661, 679, 716, 767, 2429, 2588
DpnI	GATC	9 cut(s)	53, 711, 1023, 1066, 1316, 1758, 2013, 2082, 2649
DpnII	GATC	9 cut(s)	51, 709, 1021, 1064, 1314, 1756, 2011, 2080, 2647
DraI	TTTAAA	1 cut(s)	1032
DriI	GACNNNNNGTC	1 cut(s)	976
EaeI	YGGCCR	1 cut(s)	2256
Eam1104I	CTCTTC	5 cut(s)	66, 1530, 1545, 1668, 2275
Eam1105I	GACNNNNNGTC	1 cut(s)	976
EarI	CTCTTC	5 cut(s)	66, 1530, 1545, 1668, 2275
EciI	GGCGGA	1 cut(s)	2642
Eco130I	CCWWGG	4 cut(s)	551, 2275, 2341, 2405
Eco147I	AGGCCT	2 cut(s)	1229, 2280
Eco24I	GRGCYC	1 cut(s)	1906
Eco31I	GGTCTC	2 cut(s)	47, 825
Eco32I	GATATC	2 cut(s)	1855, 2678
Eco47I	GGWCC	1 cut(s)	2389
Eco57I	CTGAAG	4 cut(s)	668, 1296, 1569, 1980
Eco81I	CCTNAGG	1 cut(s)	2006
Eco88I	CYCGRG	1 cut(s)	30
EcoRII	CCWGG	5 cut(s)	514, 603, 1229, 1410, 1566
EcoRV	GATATC	2 cut(s)	1855, 2678
EcoT14I	CCWWGG	4 cut(s)	551, 2275, 2341, 2405
EcoT22I	ATGCAT	2 cut(s)	466, 1850
EcoT38I	GRGCYC	1 cut(s)	1906
ErhI	CCWWGG	4 cut(s)	551, 2275, 2341, 2405
Esp3I	CGTCTC	2 cut(s)	27, 1609
FauI	CCCGC	3 cut(s)	1362, 1488, 2123
FauNDI	CATATG	2 cut(s)	2522, 2607
FriOI	GRGCYC	1 cut(s)	1906
FspBI	CTAG	7 cut(s)	552, 701, 1772, 2084, 2276, 2318, 2351
FspI	TGCGCA	1 cut(s)	742
GlaI	GCGC	4 cut(s)	181, 742, 1267, 1597
GsaI	CCCAGC	2 cut(s)	1902, 2615
GsuI	CTGGAG	1 cut(s)	885
HaeII	RGCGCY	1 cut(s)	1269
HaeIII	GGCC	4 cut(s)	1229, 2207, 2258, 2280
HgaI	GACGC	1 cut(s)	1390
HhaI	GCGC	4 cut(s)	182, 743, 1268, 1598
Hin6I	GCGC	4 cut(s)	180, 741, 1266, 1596
HinP1I	GCGC	4 cut(s)	180, 741, 1266, 1596
HincII	GTYRAC	1 cut(s)	97
HindII	GTYRAC	1 cut(s)	97
HindIII	AAGCTT	1 cut(s)	596
HphI	GGTGA	4 cut(s)	567, 1232, 2090, 2238
Hpy166II	GTNNAC	2 cut(s)	97, 616
Hpy8I	GTNNAC	2 cut(s)	97, 616
Hpy99I	CGWCG	3 cut(s)	13, 95, 977
HpyAV	CCTTC	6 cut(s)	93, 692, 1184, 1271, 1423, 1955
HpyCH4III	ACNGT	7 cut(s)	340, 428, 564, 884, 1531, 2330, 2597
HpyCH4IV	ACGT	2 cut(s)	1143, 2431
HpyF10VI	GCNNNNNNNGC	9 cut(s)	11, 461, 1256, 1265, 1374, 1517, 1823, 2195, 2204
HpySE526I	ACGT	2 cut(s)	1143, 2431
HspAI	GCGC	4 cut(s)	180, 741, 1266, 1596
Kzo9I	GATC	9 cut(s)	51, 709, 1021, 1064, 1314, 1756, 2011, 2080, 2647
LmnI	GCTCC	3 cut(s)	311, 1365, 1822
MaeI	CTAG	7 cut(s)	552, 701, 1772, 2084, 2276, 2318, 2351
MaeII	ACGT	2 cut(s)	1143, 2431
MaeIII	GTNAC	6 cut(s)	294, 412, 573, 816, 978, 2000
MalI	GATC	9 cut(s)	53, 711, 1023, 1066, 1316, 1758, 2013, 2082, 2649
MbiI	CCGCTC	1 cut(s)	1357
MboI	GATC	9 cut(s)	51, 709, 1021, 1064, 1314, 1756, 2011, 2080, 2647
MfeI	CAATTG	3 cut(s)	1862, 2023, 2250
MflI	RGATCY	4 cut(s)	51, 1756, 2011, 2647
MhlI	GDGCHC	1 cut(s)	1906
MlsI	TGGCCA	1 cut(s)	2258
MluNI	TGGCCA	1 cut(s)	2258
MlyI	GAGTC	6 cut(s)	256, 986, 1013, 1088, 1470, 1991
MmeI	TCCRAC	1 cut(s)	322
Mox20I	TGGCCA	1 cut(s)	2258
Mph1103I	ATGCAT	2 cut(s)	466, 1850
MroXI	GAANNNNTTC	1 cut(s)	1173
MscI	TGGCCA	1 cut(s)	2258
MslI	CAYNNNNRTG	3 cut(s)	447, 632, 1569
Msp20I	TGGCCA	1 cut(s)	2258
MspA1I	CMGCKG	2 cut(s)	317, 1873
MspR9I	CCNGG	5 cut(s)	516, 605, 1231, 1412, 1568
MunI	CAATTG	3 cut(s)	1862, 2023, 2250
MvaI	CCWGG	5 cut(s)	516, 605, 1231, 1412, 1568
MvnI	CGCG	1 cut(s)	180
MwoI	GCNNNNNNNGC	9 cut(s)	11, 461, 1256, 1265, 1374, 1517, 1823, 2195, 2204
NcoI	CCATGG	1 cut(s)	2405
NdeI	CATATG	2 cut(s)	2522, 2607
NdeII	GATC	9 cut(s)	51, 709, 1021, 1064, 1314, 1756, 2011, 2080, 2647
NlaIV	GGNNCC	1 cut(s)	2649
NmeAIII	GCCGAG	1 cut(s)	1242
NmuCI	GTSAC	2 cut(s)	573, 978
NsbI	TGCGCA	1 cut(s)	742
NsiI	ATGCAT	2 cut(s)	466, 1850
NspI	RCATGY	3 cut(s)	497, 817, 864
OliI	CACNNNNGTG	1 cut(s)	1569
PasI	CCCWGGG	1 cut(s)	604
PceI	AGGCCT	2 cut(s)	1229, 2280
PciI	ACATGT	1 cut(s)	813
PdmI	GAANNNNTTC	1 cut(s)	1173
PfeI	GAWTC	6 cut(s)	1661, 1702, 1812, 2284, 2359, 2488
PfoI	TCCNGGA	1 cut(s)	1410
PleI	GAGTC	6 cut(s)	256, 985, 1012, 1088, 1470, 1990
PpsI	GAGTC	6 cut(s)	256, 985, 1012, 1088, 1470, 1990
Ppu21I	YACGTR	1 cut(s)	2432
PscI	ACATGT	1 cut(s)	813
PshAI	GACNNNNGTC	2 cut(s)	39, 2576
PsiI	TTATAA	2 cut(s)	632, 2601
Psp6I	CCWGG	5 cut(s)	514, 603, 1229, 1410, 1566
PspFI	CCCAGC	2 cut(s)	1898, 2611
PspGI	CCWGG	5 cut(s)	514, 603, 1229, 1410, 1566
PspN4I	GGNNCC	1 cut(s)	2649
PspPI	GGNCC	2 cut(s)	2205, 2389
PstI	CTGCAG	1 cut(s)	1584
PstNI	CAGNNNCTG	4 cut(s)	1217, 1588, 1873, 2152
PsuI	RGATCY	4 cut(s)	51, 1756, 2011, 2647
PvuII	CAGCTG	1 cut(s)	1873
RsaI	GTAC	6 cut(s)	662, 680, 717, 768, 2430, 2589
RsaNI	GTAC	6 cut(s)	661, 679, 716, 767, 2429, 2588
RseI	CAYNNNNRTG	3 cut(s)	447, 632, 1569
Sau3AI	GATC	9 cut(s)	51, 709, 1021, 1064, 1314, 1756, 2011, 2080, 2647
Sau96I	GGNCC	2 cut(s)	2205, 2389
ScaI	AGTACT	2 cut(s)	768, 2589
SchI	GAGTC	6 cut(s)	256, 986, 1013, 1088, 1470, 1991
ScrFI	CCNGG	5 cut(s)	516, 605, 1231, 1412, 1568
SduI	GDGCHC	1 cut(s)	1906
SfcI	CTRYAG	3 cut(s)	336, 780, 1580
SinI	GGWCC	1 cut(s)	2389
SmiMI	CAYNNNNRTG	3 cut(s)	447, 632, 1569
SmlI	CTYRAG	2 cut(s)	264, 1697
SmoI	CTYRAG	2 cut(s)	264, 1697
SpeI	ACTAGT	1 cut(s)	2317
SseBI	AGGCCT	2 cut(s)	1229, 2280
SspI	AATATT	1 cut(s)	370
SspMI	CTAG	7 cut(s)	552, 701, 1772, 2084, 2276, 2318, 2351
StuI	AGGCCT	2 cut(s)	1229, 2280
StyD4I	CCNGG	5 cut(s)	514, 603, 1229, 1410, 1566
StyI	CCWWGG	4 cut(s)	551, 2275, 2341, 2405
TaaI	ACNGT	7 cut(s)	340, 428, 564, 884, 1531, 2330, 2597
TaiI	ACGT	2 cut(s)	1146, 2434
TaqI	TCGA	4 cut(s)	66, 855, 948, 1390
TaqII	GACCGA	1 cut(s)	2588
TatI	WGTACW	3 cut(s)	660, 766, 2587
TauI	GCSGC	2 cut(s)	8, 790
TfiI	GAWTC	6 cut(s)	1661, 1702, 1812, 2284, 2359, 2488
TscAI	CASTG	8 cut(s)	361, 594, 945, 1659, 1722, 1978, 2335, 2481
TseFI	GTSAC	2 cut(s)	573, 978
Tsp45I	GTSAC	2 cut(s)	573, 978
TspGWI	ACGGA	5 cut(s)	79, 346, 776, 1378, 1633
TspRI	CASTG	8 cut(s)	361, 594, 945, 1659, 1722, 1978, 2335, 2481
VpaK11BI	GGWCC	1 cut(s)	2389
XapI	RAATTY	7 cut(s)	866, 1111, 1204, 1349, 1621, 1744, 1919
XceI	RCATGY	3 cut(s)	497, 817, 864
XmaJI	CCTAGG	2 cut(s)	551, 2275
XmnI	GAANNNNTTC	1 cut(s)	1173
XspI	CTAG	7 cut(s)	552, 701, 1772, 2084, 2276, 2318, 2351
ZrmI	AGTACT	2 cut(s)	768, 2589
Zsp2I	ATGCAT	2 cut(s)	466, 1850

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.