Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0007704

Physical Location & Seq

Genomic Coordinates Forward (+)

39995371 .. 40007467

Gene Structure 12097 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G020020.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2637 bp

ATGGCGGCGGCGAGTGCCCCGTGGCAGCCACAGAAGGAGGGCCTCACGGCGATCTGTGGGCTGCTGGAGCAGCAGATTTCGCCGACTTCCTCCGCTGACAAGTCTCAGATTTGGCACCAACTCCAAGCCTACTCTCAGAACCCCGAGTTCCATAATTACCTTGTTTTCATTCTCACACGCGCCGAGGGTACATCAGTGGAGATTCGACAAGCGGCAGGGTTGCTTTTGAAAAACAACCTTAGAAATGCATACAGCTCCATGGATCCTGCATACCAGCAGTATATCAAGTCGGAATTACTGCCTTGCTTGGGATCACCAGATAGGCACATCAGGTCTACAGTAGGCACCATTATAAGTGTTGTTGTTCAACTCGGAGGAGTTATGGGATGGCCGGAATTACTGCAAGCTCTTGTAACTTGTTTGGACAGTAATGATGTAAATCACATGGAAGGTGCTATGGATGCATTATCCAAGGTTTGTGAGGATATACCTCAGGTGCTTGATTCAGATGTACCCGGGTTGCCTGAACGCCCCATCAACATATTCCTTCCTAGAATGCTTAAGCTTTTCCAGTCACCACATTCATCCCTCAGAAAGCTTTCCCTGGGTTCTGTAAATCAATATATTATGTTGATGCCAGCAGCTTTATATGCGTCTATGGATCAATATCTTCAGGGGTTGTTTGTCCTTGCTAATGACCCTTCTTCAGATGTGAGGAAATTGGTATGTGCAGCATTTGTTCAGCTGATTGAAGTTCGTCCAGGGTTCTTGGAGCCACAGTTGAGGAACTTAATTGAATACATGTTGCAAGTCAACAAGGACACCGATGAAGAAGTTGCCCTTGAAGCCTGTGAATTCTGGTCTGCATATTGTGAGGCTGAGTTACCACCTGAGATCTTAAGAGAGTTTTTACCCCGTCTAATTCCGATTTTGCTGTCAAACATGGCTTACGCTGAGGATGATGAGTCCCTTGTTGATGCTGAGGAGGATGGCTCTGTCCCAGACCGTGATCAGGATATCAAACCTCGTTTTCATTCATCGCGGTTTCATGGATCTGATGGTGCGGAAGAAGATGATGATGATATTGTCAATGTTTGGAATTTACGGAAATGCAGTGCAGCGGCCGTTGATATCCTGTCAACTGTGTTTGGGGATGAGATTCTCCCAACACTGATGGCCTTTGTTCAGGGAAGCTGCTGTTTTGGCTCTGGGGCGGTAGCTGAAGGTTGCATTATTGGTCTTTATCCTCATTTGAATGAGATCATTGCATATCTTATTCCTCTCTTAGACGATAAGTTTCCACTTATAAGGAGCATATCCTGTTGGACACTTTCACGTTTCAGCAAGTTCATTGTTGAGGGTGTTGGACATCAACAAGGTTATGAGCAGTTTGACAAGGTTCTTCTGGGTCTCTTAAGAAGAATATTAGATAATAACAAGCGGGTACAGGAGGCTGCTTGCTCAGCTTTTGCGACACTTGAAGAGGAGGCGGCTGATGCGTTGGCACCACGCCTGGAAACTATTTTACAACACCTTATGTGCGCTTTTGGGAAATATCAGAGACGGAACCTTAGAATTGTATATGATGCCATTGGAACTCTAGCAGATGCTGTCGGTGGGGAGTTGAATCGTCCCAATTATCTTGAAATTCTCATGCCACCATTAATTGCAAAGTGGCAACAACTTGCAAATTCAGATAAAGATTTGTTTCCATTGCTGGAGTGCTTTACTTCTATTTCGCAGGCATTGGGTGCCGGATTCTCTCCATTTGCAGAGCCAGTGTTTCAGAGGTGCATAAGCATCATACAGTCCCAACAAGTTGCCAAGGTTGATCCGGTCGCTTCAGGAGTCCAGTATGATAAAGAATTTATTGTGTGCTCTCTTGACTTGCTTTCTGGACTTACAGAAGGTCTTGGTAGTGGGATAGAAAGTTTGGTTTCACAAAGCAATTTGAAGGACCTGCTTTTGCACTGTTGCATGGATGATGCTTCGGATGTCCGACAGAGTGGCTTTGCCTTACTTGGGGATCTTGCAAGAGTATGCCCTGTTCATTTGCGTCCTCGTTTGCCGGAATTTCTTGATGTTGCAGCCAAACAACTGAATACTCCTAAGCTGAAGGAAACTGTTTCAGTGGCAAATAATGCGTGTTGGGCCATTGGTGAGTTAGCAGTCAAGGTTCATCAAGAAATTTCTCCAATTGTTTTGACGGTCATGTCTTGCTTAGTCCCCATACTGCAGCATCCAGAGGCGCTCAACAAGTCACTGATTGAGAACAGTGCTATCACGCTTGGGAGGCTTGCATATGTTTGTCCAGAGCTTGTGGCTCCGCATATGGAGCATTTCATGCAGCCATGGTGCATTGCTTTGTCAATGATACGTGATGATATAGAAAAGGAGGATGCATTCCGAGGTCTATGCGCATTGGTTAGAACAAATCCATCCGGAGCATTGAGTTCACTCATATATATGTGCAATGCTATTGCCAGTTGGCATGAAATAAGGAGTGAAGAGCTACATAATGCAGTTTGCCAGGTGTTGCACGGTTATAAGCAGATGCTTGTGAATGGAGCGTGGGAACAGTGTATGTCTGCTTTGGAGCCACAAGTCAAGAACAAGCTATCAAAATACCAAGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000060 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0017038 GO:0031090 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0033036 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0042277 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070013 GO:0070727 GO:0071702 GO:0071705 GO:0072594

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HEAT HEAT_EZ IBN_N Ribosomal_S17

Protein Analysis

878

Amino Acids

97.25

Weight (kDa)

4.88

Isoelectric Point (pI)

53.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IBN_N	PF03810	39 - 105	1.1e-11	Importin-beta N-terminal domain
TPR_IPO5	PF25780	126 - 266	1.9e-06	IPO5-like, TPR repeats
HEAT_EZ	PF13513	400 - 447	1.1e-08	HEAT-like repeat
TPR_IMB1	PF25574	467 - 682	1.7e-08	Importin subunit beta-1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007704)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16950 AT2G16950
fragaria_vesca	FvH4_2g15650 FvH4_2g15650 FvH4_2g15650 FvH4_2g15650 FvH4_2g15650 FvH4_2g15650
malus_domestica	MD05G1125000.v1.1 MD10G1127800.v1.1
prunus_persica	Prupe.8G169000_v2.0.a1 Prupe.8G169000_v2.0.a1 Prupe.8G169000_v2.0.a1
pyrus_communis	pycom05g11910 pycom10g10950
rosa_chinensis	RchiOBHm_Chr6g0278851
rosa_laevigata	RLG00000013189
rosa_multiflora	Rmu_sc0004380.1_g000009 Rmu_sc0024310.1_g000003
rosa_roxburghii	Rroxscaffold_7G00189590
rosa_rugosa	Rorug06G0119700
rosa_samantha	Rh6AG228200 Rh6BG233800
rosa_wichuraiana	Rw6G020020

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	353, 1307, 2547
AasI	GACNNNNNNGTC	1 cut(s)	2212
Acc16I	TGCGCA	1 cut(s)	2419
Acc36I	ACCTGC	1 cut(s)	1968
AccB1I	GGYRCC	4 cut(s)	114, 344, 1504, 1751
AccI	GTMKAC	1 cut(s)	335
AccII	CGCG	2 cut(s)	180, 1042
AccIII	TCCGGA	1 cut(s)	2441
AclWI	GGATC	7 cut(s)	257, 270, 319, 669, 1060, 1826, 2034
AcoI	YGGCCR	2 cut(s)	389, 1122
AcsI	RAATTY	7 cut(s)	854, 1099, 1647, 1690, 1865, 2072, 2187
AcuI	CTGAAG	5 cut(s)	656, 690, 1242, 1827, 2135
AfaI	GTAC	3 cut(s)	190, 513, 1446
AfiI	CCNNNNNNNGG	3 cut(s)	308, 1012, 2022
AflII	CTTAAG	3 cut(s)	560, 898, 1414
AflIII	ACRYGT	1 cut(s)	801
AjnI	CCWGG	4 cut(s)	603, 760, 1512, 2529
Alw21I	GWGCWC	1 cut(s)	1880
Alw26I	GTCTC	3 cut(s)	108, 1415, 1555
AlwI	GGATC	7 cut(s)	257, 270, 319, 669, 1060, 1826, 2034
AlwNI	CAGNNNCTG	1 cut(s)	1610
Ama87I	CYCGRG	2 cut(s)	143, 515
Aor13HI	TCCGGA	1 cut(s)	2441
AoxI	GGCC	5 cut(s)	40, 389, 1122, 1176, 2151
ApoI	RAATTY	7 cut(s)	854, 1099, 1647, 1690, 1865, 2072, 2187
AseI	ATTAAT	1 cut(s)	1664
Asp700I	GAANNNNTTC	1 cut(s)	598
AspLEI	GCGC	4 cut(s)	182, 1544, 2251, 2420
AspS9I	GGNCC	3 cut(s)	40, 1957, 2151
AsuC2I	CCSGG	2 cut(s)	516, 517
AsuHPI	GGTGA	3 cut(s)	306, 567, 2171
AvaI	CYCGRG	2 cut(s)	143, 515
AvaII	GGWCC	1 cut(s)	1957
AxyI	CCTNAGG	1 cut(s)	492
BaeGI	GKGCMC	1 cut(s)	19
BaeI	ACNNNNGTAYC	2 cut(s)	180, 213
BamHI	GGATCC	1 cut(s)	262
BanI	GGYRCC	4 cut(s)	114, 344, 1504, 1751
Bbv12I	GWGCWC	1 cut(s)	1880
BbvCI	CCTCAGC	2 cut(s)	954, 981
BccI	CCATC	6 cut(s)	381, 542, 983, 1052, 1168, 2446
BceAI	ACGGC	2 cut(s)	63, 1109
BcgI	CGANNNNNNTGC	2 cut(s)	2397, 2431
BciT130I	CCWGG	4 cut(s)	605, 762, 1514, 2531
BclI	TGATCA	1 cut(s)	1009
BcnI	CCSGG	2 cut(s)	516, 517
BcoDI	GTCTC	3 cut(s)	108, 1415, 1555
BfaI	CTAG	2 cut(s)	552, 1601
BfmI	CTRYAG	2 cut(s)	336, 2234
BfoI	RGCGCY	1 cut(s)	2252
BfrI	CTTAAG	3 cut(s)	560, 898, 1414
BfuAI	ACCTGC	1 cut(s)	1968
BglII	AGATCT	1 cut(s)	894
BlpI	GCTNAGC	1 cut(s)	1462
Bme1390I	CCNGG	6 cut(s)	516, 517, 605, 762, 1514, 2531
Bme18I	GGWCC	1 cut(s)	1957
BmeT110I	CYCGRG	2 cut(s)	143, 515
BmgT120I	GGNCC	3 cut(s)	40, 1957, 2151
BmiI	GGNNCC	9 cut(s)	116, 264, 346, 774, 1506, 1568, 1753, 2325, 2598
BmrFI	CCNGG	6 cut(s)	516, 517, 605, 762, 1514, 2531
BplI	GAGNNNNNCTC	2 cut(s)	977, 1009
BpmI	CTGGAG	2 cut(s)	86, 1739
Bpu10I	CCTNAGC	3 cut(s)	954, 981, 2109
Bpu1102I	GCTNAGC	1 cut(s)	1462
BpuMI	CCSGG	2 cut(s)	516, 517
BsaAI	YACGTR	1 cut(s)	2378
BsaBI	GATNNNNATC	2 cut(s)	438, 666
BsaI	GGTCTC	1 cut(s)	1415
BsaWI	WCCGGW	2 cut(s)	1834, 2441
BsaXI	ACNNNNNCTCC	2 cut(s)	764, 794
Bsc4I	CCNNNNNNNGG	3 cut(s)	308, 1012, 2022
Bse1I	ACTGG	4 cut(s)	571, 1778, 1852, 2484
Bse21I	CCTNAGG	1 cut(s)	492
Bse3DI	GCAATG	4 cut(s)	1263, 1712, 2358, 2479
Bse8I	GATNNNNATC	2 cut(s)	438, 666
BseAI	TCCGGA	1 cut(s)	2441
BseBI	CCWGG	4 cut(s)	605, 762, 1514, 2531
BseJI	GATNNNNATC	2 cut(s)	438, 666
BseLI	CCNNNNNNNGG	3 cut(s)	308, 1012, 2022
BseMI	GCAATG	4 cut(s)	1263, 1712, 2358, 2479
BseMII	CTCAG	9 cut(s)	119, 149, 506, 604, 870, 882, 945, 972, 1476
BseNI	ACTGG	4 cut(s)	571, 1778, 1852, 2484
BseRI	GAGGAG	3 cut(s)	390, 998, 1499
BseSI	GKGCMC	1 cut(s)	19
BseX3I	CGGCCG	1 cut(s)	1122
BsgI	GTGCAG	2 cut(s)	750, 1137
Bsh1236I	CGCG	2 cut(s)	180, 1042
Bsh1285I	CGRYCG	2 cut(s)	1125, 1839
BshFI	GGCC	5 cut(s)	42, 391, 1124, 1178, 2153
BshNI	GGYRCC	4 cut(s)	114, 344, 1504, 1751
BsiEI	CGRYCG	2 cut(s)	1125, 1839
BsiHKAI	GWGCWC	1 cut(s)	1880
BsiHKCI	CYCGRG	2 cut(s)	143, 515
BsiSI	CCGG	6 cut(s)	392, 516, 1755, 1835, 2069, 2442
BslFI	GGGAC	5 cut(s)	952, 983, 1617, 1795, 2210
BslI	CCNNNNNNNGG	3 cut(s)	308, 1012, 2022
BsmAI	GTCTC	3 cut(s)	108, 1415, 1555
BsmBI	CGTCTC	1 cut(s)	1555
BsmFI	GGGAC	5 cut(s)	952, 983, 1617, 1795, 2210
BsmI	GAATGC	2 cut(s)	561, 2402
BsnI	GGCC	5 cut(s)	42, 391, 1124, 1178, 2153
Bso31I	GGTCTC	1 cut(s)	1415
BsoBI	CYCGRG	2 cut(s)	143, 515
Bsp1286I	GDGCHC	2 cut(s)	19, 1880
Bsp13I	TCCGGA	1 cut(s)	2441
Bsp1720I	GCTNAGC	1 cut(s)	1462
Bsp19I	CCATGG	2 cut(s)	258, 2351
BspANI	GGCC	5 cut(s)	42, 391, 1124, 1178, 2153
BspCNI	CTCAG	9 cut(s)	118, 148, 505, 603, 871, 883, 946, 973, 1475
BspEI	TCCGGA	1 cut(s)	2441
BspFNI	CGCG	2 cut(s)	180, 1042
BspLI	GGNNCC	9 cut(s)	116, 264, 346, 774, 1506, 1568, 1753, 2325, 2598
BspMAI	CTGCAG	1 cut(s)	2238
BspMI	ACCTGC	1 cut(s)	1968
BspPI	GGATC	7 cut(s)	257, 270, 319, 669, 1060, 1826, 2034
BspQI	GCTCTTC	1 cut(s)	2502
BspT107I	GGYRCC	4 cut(s)	114, 344, 1504, 1751
BspTI	CTTAAG	3 cut(s)	560, 898, 1414
BspTNI	GGTCTC	1 cut(s)	1415
BsrDI	GCAATG	4 cut(s)	1263, 1712, 2358, 2479
BsrI	ACTGG	4 cut(s)	571, 1778, 1852, 2484
BssT1I	CCWWGG	4 cut(s)	258, 471, 1824, 2351
Bst2UI	CCWGG	4 cut(s)	605, 762, 1514, 2531
Bst6I	CTCTTC	2 cut(s)	1476, 2502
BstAFI	CTTAAG	3 cut(s)	560, 898, 1414
BstAPI	GCANNNNNTGC	3 cut(s)	1751, 2141, 2344
BstBAI	YACGTR	1 cut(s)	2378
BstC8I	GCNNGC	5 cut(s)	405, 639, 1459, 1743, 2298
BstDSI	CCRYGG	3 cut(s)	20, 258, 2351
BstFNI	CGCG	2 cut(s)	180, 1042
BstH2I	RGCGCY	1 cut(s)	2252
BstHHI	GCGC	4 cut(s)	182, 1544, 2251, 2420
BstMAI	GTCTC	3 cut(s)	108, 1415, 1555
BstMCI	CGRYCG	2 cut(s)	1125, 1839
BstNI	CCWGG	4 cut(s)	605, 762, 1514, 2531
BstNSI	RCATGY	1 cut(s)	805
BstSCI	CCNGG	6 cut(s)	514, 515, 603, 760, 1512, 2529
BstSFI	CTRYAG	2 cut(s)	336, 2234
BstSLI	GKGCMC	1 cut(s)	19
BstUI	CGCG	2 cut(s)	180, 1042
BstX2I	RGATCY	4 cut(s)	262, 894, 1052, 2026
BstYI	RGATCY	4 cut(s)	262, 894, 1052, 2026
BstZI	CGGCCG	1 cut(s)	1122
Bsu36I	CCTNAGG	1 cut(s)	492
BsuRI	GGCC	5 cut(s)	42, 391, 1124, 1178, 2153
BtgI	CCRYGG	3 cut(s)	20, 258, 2351
BtgZI	GCGATG	1 cut(s)	1023
BtsI	GCAGTG	1 cut(s)	1120
BtsIMutI	CAGTG	9 cut(s)	201, 1120, 1169, 1785, 1969, 2136, 2261, 2281, 2585
BveI	ACCTGC	1 cut(s)	1968
Cac8I	GCNNGC	5 cut(s)	405, 639, 1459, 1743, 2298
CaiI	CAGNNNCTG	1 cut(s)	1610
CfoI	GCGC	4 cut(s)	182, 1544, 2251, 2420
Cfr13I	GGNCC	3 cut(s)	40, 1957, 2151
Cfr9I	CCCGGG	1 cut(s)	515
CseI	GACGC	2 cut(s)	642, 2045
Csp6I	GTAC	3 cut(s)	189, 512, 1445
CviQI	GTAC	3 cut(s)	189, 512, 1445
DrdI	GACNNNNNNGTC	1 cut(s)	2212
DseDI	GACNNNNNNGTC	1 cut(s)	2212
EaeI	YGGCCR	2 cut(s)	389, 1122
EagI	CGGCCG	1 cut(s)	1122
Eam1104I	CTCTTC	2 cut(s)	1476, 2502
EarI	CTCTTC	2 cut(s)	1476, 2502
EclXI	CGGCCG	1 cut(s)	1122
Eco130I	CCWWGG	4 cut(s)	258, 471, 1824, 2351
Eco31I	GGTCTC	1 cut(s)	1415
Eco32I	GATATC	2 cut(s)	1018, 1132
Eco47I	GGWCC	1 cut(s)	1957
Eco52I	CGGCCG	1 cut(s)	1122
Eco57I	CTGAAG	5 cut(s)	656, 690, 1242, 1827, 2135
Eco81I	CCTNAGG	1 cut(s)	492
Eco88I	CYCGRG	2 cut(s)	143, 515
EcoO109I	RGGNCCY	2 cut(s)	40, 1957
EcoRI	GAATTC	1 cut(s)	854
EcoRII	CCWGG	4 cut(s)	603, 760, 1512, 2529
EcoRV	GATATC	2 cut(s)	1018, 1132
EcoT14I	CCWWGG	4 cut(s)	258, 471, 1824, 2351
EcoT22I	ATGCAT	3 cut(s)	250, 466, 2404
ErhI	CCWWGG	4 cut(s)	258, 471, 1824, 2351
Esp3I	CGTCTC	1 cut(s)	1555
FalI	AAGNNNNNCTT	2 cut(s)	825, 857
FaqI	GGGAC	5 cut(s)	952, 983, 1617, 1795, 2210
FauI	CCCGC	1 cut(s)	1434
FauNDI	CATATG	2 cut(s)	2302, 2331
FbaI	TGATCA	1 cut(s)	1009
FblI	GTMKAC	1 cut(s)	335
FspAI	RTGCGCAY	1 cut(s)	2419
FspBI	CTAG	2 cut(s)	552, 1601
FspI	TGCGCA	1 cut(s)	2419
GlaI	GCGC	4 cut(s)	181, 1543, 2250, 2419
GsuI	CTGGAG	2 cut(s)	86, 1739
HaeII	RGCGCY	1 cut(s)	2252
HaeIII	GGCC	5 cut(s)	42, 391, 1124, 1178, 2153
HapII	CCGG	6 cut(s)	392, 516, 1755, 1835, 2069, 2442
HgaI	GACGC	2 cut(s)	642, 2045
HhaI	GCGC	4 cut(s)	182, 1544, 2251, 2420
Hin6I	GCGC	4 cut(s)	180, 1542, 2249, 2418
HinP1I	GCGC	4 cut(s)	180, 1542, 2249, 2418
HincII	GTYRAC	2 cut(s)	814, 1140
HindII	GTYRAC	2 cut(s)	814, 1140
HindIII	AAGCTT	2 cut(s)	563, 596
HinfI	GANTC	7 cut(s)	202, 503, 965, 1159, 1627, 1758, 1848
HpaII	CCGG	6 cut(s)	392, 516, 1755, 1835, 2069, 2442
HphI	GGTGA	3 cut(s)	306, 567, 2171
Hpy166II	GTNNAC	4 cut(s)	336, 814, 1140, 2456
Hpy8I	GTNNAC	4 cut(s)	336, 814, 1140, 2456
HpyAV	CCTTC	8 cut(s)	28, 443, 557, 711, 1217, 1901, 1948, 2110
HpyCH4IV	ACGT	2 cut(s)	1336, 2377
HpySE526I	ACGT	2 cut(s)	1336, 2377
HspAI	GCGC	4 cut(s)	180, 1542, 2249, 2418
Kpn2I	TCCGGA	1 cut(s)	2441
Ksp22I	TGATCA	1 cut(s)	1009
LguI	GCTCTTC	1 cut(s)	2502
LmnI	GCTCC	9 cut(s)	67, 260, 772, 1311, 2329, 2335, 2444, 2567, 2596
MaeI	CTAG	2 cut(s)	552, 1601
MaeII	ACGT	2 cut(s)	1336, 2377
MaeIII	GTNAC	4 cut(s)	412, 573, 882, 2259
MboII	GAAGA	9 cut(s)	662, 696, 842, 1079, 1082, 1394, 1431, 1493, 2519
MfeI	CAATTG	1 cut(s)	2196
MflI	RGATCY	4 cut(s)	262, 894, 1052, 2026
MhlI	GDGCHC	2 cut(s)	19, 1880
MlyI	GAGTC	2 cut(s)	974, 1857
MmeI	TCCRAC	4 cut(s)	270, 1304, 1345, 2023
Mph1103I	ATGCAT	3 cut(s)	250, 466, 2404
MroI	TCCGGA	1 cut(s)	2441
MroXI	GAANNNNTTC	1 cut(s)	598
MseI	TTAA	5 cut(s)	561, 791, 899, 1415, 1664
MslI	CAYNNNNRTG	2 cut(s)	1254, 2465
MspA1I	CMGCKG	3 cut(s)	95, 745, 1121
MspCI	CTTAAG	3 cut(s)	560, 898, 1414
MspI	CCGG	6 cut(s)	392, 516, 1755, 1835, 2069, 2442
MspR9I	CCNGG	6 cut(s)	516, 517, 605, 762, 1514, 2531
MunI	CAATTG	1 cut(s)	2196
Mva1269I	GAATGC	2 cut(s)	561, 2402
MvaI	CCWGG	4 cut(s)	605, 762, 1514, 2531
MvnI	CGCG	2 cut(s)	180, 1042
NciI	CCSGG	2 cut(s)	516, 517
NcoI	CCATGG	2 cut(s)	258, 2351
NdeI	CATATG	2 cut(s)	2302, 2331
NlaIV	GGNNCC	9 cut(s)	116, 264, 346, 774, 1506, 1568, 1753, 2325, 2598
NmeAIII	GCCGAG	1 cut(s)	208
NmuCI	GTSAC	2 cut(s)	573, 2259
NsbI	TGCGCA	1 cut(s)	2419
NsiI	ATGCAT	3 cut(s)	250, 466, 2404
NspI	RCATGY	1 cut(s)	805
PasI	CCCWGGG	1 cut(s)	604
PciI	ACATGT	1 cut(s)	801
PciSI	GCTCTTC	1 cut(s)	2502
PctI	GAATGC	2 cut(s)	561, 2402
PdmI	GAANNNNTTC	1 cut(s)	598
PfeI	GAWTC	5 cut(s)	202, 503, 1159, 1627, 1758
PleI	GAGTC	2 cut(s)	973, 1856
PpsI	GAGTC	2 cut(s)	973, 1856
Ppu21I	YACGTR	1 cut(s)	2378
PpuMI	RGGWCCY	1 cut(s)	1957
PscI	ACATGT	1 cut(s)	801
PshBI	ATTAAT	1 cut(s)	1664
PsiI	TTATAA	3 cut(s)	353, 1307, 2547
Psp5II	RGGWCCY	1 cut(s)	1957
Psp6I	CCWGG	4 cut(s)	603, 760, 1512, 2529
PspGI	CCWGG	4 cut(s)	603, 760, 1512, 2529
PspN4I	GGNNCC	9 cut(s)	116, 264, 346, 774, 1506, 1568, 1753, 2325, 2598
PspPI	GGNCC	3 cut(s)	40, 1957, 2151
PspPPI	RGGWCCY	1 cut(s)	1957
PsrI	GAACNNNNNNTAC	2 cut(s)	2031, 2063
PstI	CTGCAG	1 cut(s)	2238
PstNI	CAGNNNCTG	1 cut(s)	1610
PsuI	RGATCY	4 cut(s)	262, 894, 1052, 2026
PvuII	CAGCTG	1 cut(s)	745
RsaI	GTAC	3 cut(s)	190, 513, 1446
RsaNI	GTAC	3 cut(s)	189, 512, 1445
RseI	CAYNNNNRTG	2 cut(s)	1254, 2465
SapI	GCTCTTC	1 cut(s)	2502
SaqAI	TTAA	5 cut(s)	561, 791, 899, 1415, 1664
Sau96I	GGNCC	3 cut(s)	40, 1957, 2151
SchI	GAGTC	2 cut(s)	974, 1857
ScrFI	CCNGG	6 cut(s)	516, 517, 605, 762, 1514, 2531
SduI	GDGCHC	2 cut(s)	19, 1880
SfcI	CTRYAG	2 cut(s)	336, 2234
SinI	GGWCC	1 cut(s)	1957
SmaI	CCCGGG	1 cut(s)	517
SmiMI	CAYNNNNRTG	2 cut(s)	1254, 2465
SmlI	CTYRAG	3 cut(s)	560, 898, 1414
SmoI	CTYRAG	3 cut(s)	560, 898, 1414
SspI	AATATT	1 cut(s)	1425
SspMI	CTAG	2 cut(s)	552, 1601
StyD4I	CCNGG	6 cut(s)	514, 515, 603, 760, 1512, 2529
StyI	CCWWGG	4 cut(s)	258, 471, 1824, 2351
TaiI	ACGT	2 cut(s)	1339, 2380
TaqI	TCGA	1 cut(s)	205
TauI	GCSGC	5 cut(s)	8, 11, 215, 1124, 1493
TfiI	GAWTC	5 cut(s)	202, 503, 1159, 1627, 1758
Tru1I	TTAA	5 cut(s)	561, 791, 899, 1415, 1664
Tru9I	TTAA	5 cut(s)	561, 791, 899, 1415, 1664
TscAI	CASTG	9 cut(s)	201, 1120, 1176, 1785, 1976, 2136, 2268, 2281, 2585
TseFI	GTSAC	2 cut(s)	573, 2259
Tsp45I	GTSAC	2 cut(s)	573, 2259
TspGWI	ACGGA	2 cut(s)	1120, 1579
TspMI	CCCGGG	1 cut(s)	515
TspRI	CASTG	9 cut(s)	201, 1120, 1176, 1785, 1976, 2136, 2268, 2281, 2585
Vha464I	CTTAAG	3 cut(s)	560, 898, 1414
VpaK11BI	GGWCC	1 cut(s)	1957
VspI	ATTAAT	1 cut(s)	1664
XapI	RAATTY	7 cut(s)	854, 1099, 1647, 1690, 1865, 2072, 2187
XceI	RCATGY	1 cut(s)	805
XmaI	CCCGGG	1 cut(s)	515
XmiI	GTMKAC	1 cut(s)	335
XmnI	GAANNNNTTC	1 cut(s)	598
XspI	CTAG	2 cut(s)	552, 1601
Zsp2I	ATGCAT	3 cut(s)	250, 466, 2404

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.