Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0007704

Physical Location & Seq

Genomic Coordinates Forward (+)

20796704 .. 20809529

Gene Structure 12826 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1127800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2697 bp

ATGGCGGCGACCGCCCCATGGCAGCCCAAGGAAGAAGGATTCGCGGCGATCTGCGGGCTGCTGGAGCAGCAGATTTCGCATTCGGCTTCCTCCGCTGACAAGTCTCAGATTTGGCAGCAGCTCCAACACTACTCTCAGTTCCCCGATTTCAATAACTACCTTGCGTTCATTCTTGCACGTGCGGAGGGTAAATCAGTGGAGGTTCGACAGGCAGCGGGATTGCTTTTGAAGAATAATCTTAAAAATGCTTATACTTCCATGGCTCCTGCATACCAGCAATATATAAAATCGGAATTATTGCCTTGCTTAGGAGTAGCAGACCGACACATCAGGTGTACAGTTGGGACCATAATAAGCGTTGTTGTTCAGCTAGGGGGAATTTTGGGGTGGCCTGAACTATTGCAAGCTCTTGTAAGTTGTTTAGACACTAATGACCTAAATCACATGGAAGGTGCTATGGACACATTATCCAAGATATGTGAGGATATACCTCAAGTGCTTGATTCAGATGTACCTGGATTGCCTGAACGTCCCATCAACGTATTCCTTCCGAGATTACTTAAGTTTTTCCAATCACCACATGCATCGCTTAGAAAGCTTTCCTTGGGTTCTGTAAATCAATACATTATGTTGTTGCCAGCTGCTTTGCATGCATCCATGGATCAGTATCTTCAAGGTTTGTTTATCCTTGCTAATGACCCGTCTTCAGAAGTGAGGAAATTGGTTAGTGCAGCATTTGTCCAGCTAATTGAAGTCCGTCCAGCATTCTTGGAGCCACATTTAAGGAATGTAATTGAATACATGTTGCAAGTCAACAACGACACTGAGGAAGAAGTGGCTCTTGAAGCCTGTGAATTTTGGTCTGCATATTGTGAAGCTCAGTTACCGTCTGAGAACTTAAGAGAGTTTTTGCCACGCCTAATTCCAATTTTGTTATCAAACATGGTTTATTCTGACGATGATGAGTCCCTTGCTGATGCTGAGGAGGATGGATCTGTCCCAGACCGTGATCAGGATATCAAACCTCGGTTTCATTCATCACGACTTCATGGATCTGAGAACGGGGAAGATGATGATGATAATATTGTTGGTATATGGAATTTACGCAAATGCAGTGCAGCAGCTCTCGATATCCTGTCAAATGTGTTTGGCGATGAGATTCTCCCAACGCTGATGCCCTTTGTTCAGGCCAAGTTGGCCACCAGTGGTGATGAAACCTGGAAAGGAAGGGAAGCTGCTGTTTTGGCTCTTGGTGCCATAGCTGAAGGTTGCATCAGTGGTCTTTATCCTCATTTGAATGAGATTGTTGCATTTTTAATCCCCCTTTTAGACGATAAGTTTCCACTTATAAGGAGTATTTCTTGTTGGACACTTTCTCGTTTCAGCAAGTTCATTGTTCAGAGTGCTGGACATCAAAAAGGTTATGAGCAATTTGACAAGGCTCTTGTGGGTCTATTAAGAAGAATACTAGATAATAACAAGCGGGTGCAAGAGGCTGCGTGCTCTGCTTTTGCAACTCTAGAAGAGGAGGCAGCAGAAGAGTTGGCACCGCGTTTGGAAATGATTTTACAACACCTTATGTGTGCTTTTGGGAAATATCAGAGGAGGAACCTCAGAATTGTATACGATGCTATTGGAACTCTAGCAGATGCTGTTGGTGGAGAGTTGAATCAGCCCACTTATCTTGAAATTCTGATGCCACCATTAATTGCAAAGTGGCAACAACTTTCAAATTCAGATAAAGATCTGTTTCCATTGCTAGAGTGCTTTACTTCTATATCACAGGCATTGGGTGCCGGGTTCTCTCAGTTTGCTGAACCTGTATTTCAGAGATGCATAAGCATCATTCAGTCCCAACAACTTGCAAAGGTTCAGGCAAAGGTCAATACTGTACCCTCCGGGGTCCTGTATGATAAAGAATTTATTGTTTGCTCTCTTGATCTGCTTTCTGGACTTGCGGAAGGTCTTGGCAGTGGGATAGAGAGTTTGGTTTCACAAAGCAATTTGAGGGACCTGCTTTTGCAATGTTGCACGGATGAGGCTTCGGATGTCCGACAGAGTGGATTTGCACTACTTGGGGATCTTGCAAGAGTATGCCCAGTTCATTTGCAACCTCGTTTGCCAGAATTTCTTGATGTTGCAGCCAAGCAACTGAATACTCCTAAGCTGCAAGAAACTGTTTCAGTTGCAAACAATGCATGTTGGGCCATTGGAGAGTTAGCAGTTAAGGTTCGTCAAGAAATTTCTCCAATTGTTCTGACAGTTATTTCCTGCTTAGTTCAAATCCTTCAACACGCAGAGGAGCTGAACAAGTCACTAATTGAAAACAGTGCTATCACCCTTGGGAGGATTGCGTGGGTGTGTCCAGAGCTTGTGGGTCCCCATATGGAGCATTTCATGCAACGATGGTGCATTGCTTTGTCAATGATACGTGATGATATAGAAAAGGAGGATGCCTTCCGAGGTCTATGTGCTTTGGTTAGAACCAATCCATCCGGAGCATTGAATTCACTCATTTATCTTTGCAATGCTATTGCAAGTTGGCATGAAATAAAGAGCGAAGAGCTTCATAATGAAGTTCACCAGGTGTTGCATGGTTATAAGCAGATGCTTGTGAATGGCGGAGCGTGGGACCAGTGTATGTCTGCCTTGGAGCCACCAGTGAGGGAAAAGCTTTCAAAAATATACCAAGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000060 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0017038 GO:0031090 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0033036 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0042277 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070013 GO:0070727 GO:0071702 GO:0071705 GO:0072594

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HEAT HEAT_EZ IBN_N Ribosomal_S17

Protein Analysis

899

Amino Acids

99.6

Weight (kDa)

4.95

Isoelectric Point (pI)

49.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IBN_N	PF03810	38 - 104	2.8e-12	Importin-beta N-terminal domain
HEAT_EZ	PF13513	410 - 461	6.4e-14	HEAT-like repeat
TPR_IMB1	PF25574	491 - 700	1.8e-07	Importin subunit beta-1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007704)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16950 AT2G16950
fragaria_vesca	FvH4_2g15650 FvH4_2g15650 FvH4_2g15650 FvH4_2g15650 FvH4_2g15650 FvH4_2g15650
malus_domestica	MD05G1125000.v1.1 MD10G1127800.v1.1
prunus_persica	Prupe.8G169000_v2.0.a1 Prupe.8G169000_v2.0.a1 Prupe.8G169000_v2.0.a1
pyrus_communis	pycom05g11910 pycom10g10950
rosa_chinensis	RchiOBHm_Chr6g0278851
rosa_laevigata	RLG00000013189
rosa_multiflora	Rmu_sc0004380.1_g000009 Rmu_sc0024310.1_g000003
rosa_roxburghii	Rroxscaffold_7G00189590
rosa_rugosa	Rorug06G0119700
rosa_samantha	Rh6AG228200 Rh6BG233800
rosa_wichuraiana	Rw6G020020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1349, 2601
Acc36I	ACCTGC	1 cut(s)	2022
AccB1I	GGYRCC	3 cut(s)	1253, 1546, 1793
AccI	GTMKAC	1 cut(s)	1623
AccII	CGCG	2 cut(s)	44, 1552
AccIII	TCCGGA	1 cut(s)	2495
AclWI	GGATC	4 cut(s)	669, 1000, 1060, 2088
AcoI	YGGCCR	1 cut(s)	1197
AcsI	RAATTY	9 cut(s)	378, 854, 1099, 1689, 1732, 1919, 2126, 2241, 2506
AcuI	CTGAAG	2 cut(s)	690, 1284
AcvI	CACGTG	1 cut(s)	179
AdeI	CACNNNGTG	1 cut(s)	2587
AfaI	GTAC	3 cut(s)	337, 513, 1893
AfiI	CCNNNNNNNGG	4 cut(s)	18, 308, 1012, 2346
AflII	CTTAAG	2 cut(s)	560, 898
AflIII	ACRYGT	1 cut(s)	801
AjnI	CCWGG	3 cut(s)	514, 1217, 2583
Alw21I	GWGCWC	1 cut(s)	1505
Alw26I	GTCTC	1 cut(s)	108
AlwI	GGATC	4 cut(s)	669, 1000, 1060, 2088
AlwNI	CAGNNNCTG	1 cut(s)	1652
Aor13HI	TCCGGA	1 cut(s)	2495
AoxI	GGCC	4 cut(s)	389, 1188, 1197, 2205
ApoI	RAATTY	9 cut(s)	378, 854, 1099, 1689, 1732, 1919, 2126, 2241, 2506
AseI	ATTAAT	1 cut(s)	1706
Asp700I	GAANNNNTTC	2 cut(s)	598, 2565
AspS9I	GGNCC	6 cut(s)	345, 1903, 2011, 2205, 2378, 2632
AsuC2I	CCSGG	2 cut(s)	1798, 1900
AsuHPI	GGTGA	4 cut(s)	567, 1220, 2329, 2573
AvaII	GGWCC	5 cut(s)	345, 1903, 2011, 2378, 2632
BalI	TGGCCA	1 cut(s)	1199
BanI	GGYRCC	3 cut(s)	1253, 1546, 1793
BarI	GAAGNNNNNNTAC	2 cut(s)	867, 899
BbrPI	CACGTG	1 cut(s)	179
BbsI	GAAGAC	1 cut(s)	696
Bbv12I	GWGCWC	1 cut(s)	1505
BbvCI	CCTCAGC	1 cut(s)	981
BccI	CCATC	4 cut(s)	542, 983, 2400, 2500
BciT130I	CCWGG	3 cut(s)	516, 1219, 2585
BclI	TGATCA	1 cut(s)	1009
BcnI	CCSGG	2 cut(s)	1798, 1900
BcoDI	GTCTC	1 cut(s)	108
BfaI	CTAG	5 cut(s)	371, 1469, 1520, 1643, 1760
BfrI	CTTAAG	2 cut(s)	560, 898
BfuAI	ACCTGC	1 cut(s)	2022
BglI	GCCNNNNNGGC	1 cut(s)	1196
BglII	AGATCT	1 cut(s)	1744
Bme1390I	CCNGG	5 cut(s)	516, 1219, 1798, 1900, 2585
Bme18I	GGWCC	5 cut(s)	345, 1903, 2011, 2378, 2632
BmgT120I	GGNCC	6 cut(s)	345, 1903, 2011, 2205, 2378, 2632
BmrFI	CCNGG	5 cut(s)	516, 1219, 1798, 1900, 2585
BmrI	ACTGGG	1 cut(s)	2093
BmuI	ACTGGG	1 cut(s)	2093
BpiI	GAAGAC	1 cut(s)	696
BpmI	CTGGAG	1 cut(s)	83
Bpu10I	CCTNAGC	3 cut(s)	307, 981, 2163
BpuEI	CTTGAG	1 cut(s)	477
BpuMI	CCSGG	2 cut(s)	1798, 1900
BsaAI	YACGTR	2 cut(s)	179, 2432
BsaBI	GATNNNNATC	2 cut(s)	666, 1743
BsaWI	WCCGGW	1 cut(s)	2495
Bsc4I	CCNNNNNNNGG	4 cut(s)	18, 308, 1012, 2346
Bse1I	ACTGG	4 cut(s)	1203, 2099, 2635, 2660
Bse3DI	GCAATG	4 cut(s)	1754, 2030, 2412, 2533
Bse8I	GATNNNNATC	2 cut(s)	666, 1743
BseAI	TCCGGA	1 cut(s)	2495
BseBI	CCWGG	3 cut(s)	516, 1219, 2585
BseGI	GGATG	6 cut(s)	653, 994, 2041, 2053, 2458, 2492
BseJI	GATNNNNATC	2 cut(s)	666, 1743
BseLI	CCNNNNNNNGG	4 cut(s)	18, 308, 1012, 2346
BseMI	GCAATG	4 cut(s)	1754, 2030, 2412, 2533
BseMII	CTCAG	9 cut(s)	119, 149, 816, 882, 893, 972, 1047, 1627, 1820
BseNI	ACTGG	4 cut(s)	1203, 2099, 2635, 2660
BseRI	GAGGAG	4 cut(s)	998, 1541, 1618, 2315
BsgI	GTGCAG	2 cut(s)	750, 1137
Bsh1236I	CGCG	2 cut(s)	44, 1552
Bsh1285I	CGRYCG	1 cut(s)	12
BshFI	GGCC	4 cut(s)	391, 1190, 1199, 2207
BshNI	GGYRCC	3 cut(s)	1253, 1546, 1793
BsiEI	CGRYCG	1 cut(s)	12
BsiHKAI	GWGCWC	1 cut(s)	1505
BsiSI	CCGG	3 cut(s)	1797, 1899, 2496
BslFI	GGGAC	8 cut(s)	358, 516, 952, 983, 1837, 2024, 2364, 2645
BslI	CCNNNNNNNGG	4 cut(s)	18, 308, 1012, 2346
BsmAI	GTCTC	1 cut(s)	108
BsmFI	GGGAC	8 cut(s)	358, 516, 952, 983, 1837, 2024, 2364, 2645
BsmI	GAATGC	2 cut(s)	79, 764
BsnI	GGCC	4 cut(s)	391, 1190, 1199, 2207
Bsp1286I	GDGCHC	1 cut(s)	1505
Bsp13I	TCCGGA	1 cut(s)	2495
Bsp1407I	TGTACA	1 cut(s)	335
Bsp143I	GATC	8 cut(s)	48, 661, 992, 1009, 1052, 1744, 1939, 2080
Bsp19I	CCATGG	3 cut(s)	17, 258, 657
BspANI	GGCC	4 cut(s)	391, 1190, 1199, 2207
BspCNI	CTCAG	9 cut(s)	118, 148, 817, 883, 892, 973, 1048, 1626, 1819
BspEI	TCCGGA	1 cut(s)	2495
BspFNI	CGCG	2 cut(s)	44, 1552
BspMI	ACCTGC	1 cut(s)	2022
BspPI	GGATC	4 cut(s)	669, 1000, 1060, 2088
BspQI	GCTCTTC	1 cut(s)	2556
BspT107I	GGYRCC	3 cut(s)	1253, 1546, 1793
BspTI	CTTAAG	2 cut(s)	560, 898
BsrDI	GCAATG	4 cut(s)	1754, 2030, 2412, 2533
BsrGI	TGTACA	1 cut(s)	335
BsrI	ACTGG	4 cut(s)	1203, 2099, 2635, 2660
BssMI	GATC	8 cut(s)	48, 661, 992, 1009, 1052, 1744, 1939, 2080
BssNAI	GTATAC	1 cut(s)	1624
BssT1I	CCWWGG	7 cut(s)	17, 27, 258, 603, 657, 2341, 2649
Bst1107I	GTATAC	1 cut(s)	1624
Bst2UI	CCWGG	3 cut(s)	516, 1219, 2585
Bst4CI	ACNGT	7 cut(s)	340, 888, 1007, 1891, 2179, 2263, 2330
Bst6I	CTCTTC	3 cut(s)	1518, 1533, 2556
BstAFI	CTTAAG	2 cut(s)	560, 898
BstAPI	GCANNNNNTGC	3 cut(s)	1793, 2195, 2398
BstAUI	TGTACA	1 cut(s)	335
BstBAI	YACGTR	2 cut(s)	179, 2432
BstC8I	GCNNGC	5 cut(s)	56, 405, 639, 651, 1501
BstDSI	CCRYGG	3 cut(s)	17, 258, 657
BstENI	CCTNNNNNAGG	1 cut(s)	306
BstF5I	GGATG	6 cut(s)	653, 994, 2041, 2053, 2458, 2492
BstFNI	CGCG	2 cut(s)	44, 1552
BstKTI	GATC	8 cut(s)	51, 664, 995, 1012, 1055, 1747, 1942, 2083
BstMAI	GTCTC	1 cut(s)	108
BstMBI	GATC	8 cut(s)	48, 661, 992, 1009, 1052, 1744, 1939, 2080
BstMCI	CGRYCG	1 cut(s)	12
BstNI	CCWGG	3 cut(s)	516, 1219, 2585
BstNSI	RCATGY	4 cut(s)	584, 653, 805, 2202
BstSCI	CCNGG	5 cut(s)	514, 1217, 1796, 1898, 2583
BstUI	CGCG	2 cut(s)	44, 1552
BstV2I	GAAGAC	1 cut(s)	696
BstX2I	RGATCY	4 cut(s)	992, 1052, 1744, 2080
BstYI	RGATCY	4 cut(s)	992, 1052, 1744, 2080
BstZ17I	GTATAC	1 cut(s)	1624
BsuRI	GGCC	4 cut(s)	391, 1190, 1199, 2207
BtgI	CCRYGG	3 cut(s)	17, 258, 657
BtgZI	GCGATG	2 cut(s)	570, 1167
BtsCI	GGATG	6 cut(s)	653, 994, 2041, 2053, 2458, 2492
BtsI	GCAGTG	2 cut(s)	1120, 1978
BtsIMutI	CAGTG	9 cut(s)	201, 822, 1120, 1210, 1282, 1978, 2335, 2642, 2667
BveI	ACCTGC	1 cut(s)	2022
Cac8I	GCNNGC	5 cut(s)	56, 405, 639, 651, 1501
CaiI	CAGNNNCTG	1 cut(s)	1652
Cfr13I	GGNCC	6 cut(s)	345, 1903, 2011, 2205, 2378, 2632
CsiI	ACCWGGT	1 cut(s)	2583
Csp6I	GTAC	3 cut(s)	336, 512, 1892
CspCI	CAANNNNNGTGG	2 cut(s)	1666, 1701
CviQI	GTAC	3 cut(s)	336, 512, 1892
DpnI	GATC	8 cut(s)	50, 663, 994, 1011, 1054, 1746, 1941, 2082
DpnII	GATC	8 cut(s)	48, 661, 992, 1009, 1052, 1744, 1939, 2080
DraIII	CACNNNGTG	1 cut(s)	2587
EaeI	YGGCCR	1 cut(s)	1197
Eam1104I	CTCTTC	3 cut(s)	1518, 1533, 2556
EarI	CTCTTC	3 cut(s)	1518, 1533, 2556
EciI	GGCGGA	1 cut(s)	2637
Eco130I	CCWWGG	7 cut(s)	17, 27, 258, 603, 657, 2341, 2649
Eco32I	GATATC	2 cut(s)	1018, 1132
Eco47I	GGWCC	5 cut(s)	345, 1903, 2011, 2378, 2632
Eco57I	CTGAAG	2 cut(s)	690, 1284
Eco72I	CACGTG	1 cut(s)	179
EcoNI	CCTNNNNNAGG	1 cut(s)	306
EcoO109I	RGGNCCY	3 cut(s)	1903, 2011, 2378
EcoRI	GAATTC	1 cut(s)	2506
EcoRII	CCWGG	3 cut(s)	514, 1217, 2583
EcoRV	GATATC	2 cut(s)	1018, 1132
EcoT14I	CCWWGG	7 cut(s)	17, 27, 258, 603, 657, 2341, 2649
EcoT22I	ATGCAT	4 cut(s)	586, 655, 1838, 2200
ErhI	CCWWGG	7 cut(s)	17, 27, 258, 603, 657, 2341, 2649
FalI	AAGNNNNNCTT	4 cut(s)	587, 619, 825, 857
FaqI	GGGAC	8 cut(s)	358, 516, 952, 983, 1837, 2024, 2364, 2645
FauI	CCCGC	3 cut(s)	47, 208, 1476
FauNDI	CATATG	1 cut(s)	2385
FbaI	TGATCA	1 cut(s)	1009
FblI	GTMKAC	1 cut(s)	1623
FokI	GGATG	6 cut(s)	640, 1001, 2048, 2060, 2465, 2479
FspBI	CTAG	5 cut(s)	371, 1469, 1520, 1643, 1760
GsuI	CTGGAG	1 cut(s)	83
HaeIII	GGCC	4 cut(s)	391, 1190, 1199, 2207
HapII	CCGG	3 cut(s)	1797, 1899, 2496
HincII	GTYRAC	1 cut(s)	814
HindII	GTYRAC	1 cut(s)	814
HindIII	AAGCTT	2 cut(s)	596, 2672
HinfI	GANTC	5 cut(s)	39, 503, 965, 1159, 1669
HpaII	CCGG	3 cut(s)	1797, 1899, 2496
HphI	GGTGA	4 cut(s)	567, 1220, 2329, 2573
Hpy166II	GTNNAC	4 cut(s)	336, 814, 1624, 2581
Hpy8I	GTNNAC	4 cut(s)	336, 814, 1624, 2581
HpyAV	CCTTC	8 cut(s)	29, 443, 557, 1221, 1259, 1955, 2297, 2467
HpyCH4III	ACNGT	7 cut(s)	340, 888, 1007, 1891, 2179, 2263, 2330
HpyCH4IV	ACGT	4 cut(s)	178, 529, 540, 2431
HpySE526I	ACGT	4 cut(s)	178, 529, 540, 2431
KflI	GGGWCCC	1 cut(s)	2378
Kpn2I	TCCGGA	1 cut(s)	2495
Ksp22I	TGATCA	1 cut(s)	1009
Kzo9I	GATC	8 cut(s)	48, 661, 992, 1009, 1052, 1744, 1939, 2080
LguI	GCTCTTC	1 cut(s)	2556
LmnI	GCTCC	9 cut(s)	64, 126, 268, 772, 2302, 2389, 2498, 2624, 2653
MabI	ACCWGGT	1 cut(s)	2583
MaeI	CTAG	5 cut(s)	371, 1469, 1520, 1643, 1760
MaeII	ACGT	4 cut(s)	178, 529, 540, 2431
MaeIII	GTNAC	2 cut(s)	882, 2313
MalI	GATC	8 cut(s)	50, 663, 994, 1011, 1054, 1746, 1941, 2082
MboI	GATC	8 cut(s)	48, 661, 992, 1009, 1052, 1744, 1939, 2080
MfeI	CAATTG	1 cut(s)	2250
MflI	RGATCY	4 cut(s)	992, 1052, 1744, 2080
MhlI	GDGCHC	1 cut(s)	1505
MlsI	TGGCCA	1 cut(s)	1199
MluNI	TGGCCA	1 cut(s)	1199
MlyI	GAGTC	1 cut(s)	974
MmeI	TCCRAC	3 cut(s)	148, 1346, 2077
Mox20I	TGGCCA	1 cut(s)	1199
Mph1103I	ATGCAT	4 cut(s)	586, 655, 1838, 2200
MroI	TCCGGA	1 cut(s)	2495
MroXI	GAANNNNTTC	2 cut(s)	598, 2565
MscI	TGGCCA	1 cut(s)	1199
MseI	TTAA	8 cut(s)	240, 561, 782, 899, 1316, 1457, 1706, 2226
MslI	CAYNNNNRTG	1 cut(s)	1296
Msp20I	TGGCCA	1 cut(s)	1199
MspA1I	CMGCKG	3 cut(s)	95, 215, 641
MspCI	CTTAAG	2 cut(s)	560, 898
MspI	CCGG	3 cut(s)	1797, 1899, 2496
MspR9I	CCNGG	5 cut(s)	516, 1219, 1798, 1900, 2585
MunI	CAATTG	1 cut(s)	2250
Mva1269I	GAATGC	2 cut(s)	79, 764
MvaI	CCWGG	3 cut(s)	516, 1219, 2585
MvnI	CGCG	2 cut(s)	44, 1552
NciI	CCSGG	2 cut(s)	1798, 1900
NcoI	CCATGG	3 cut(s)	17, 258, 657
NdeI	CATATG	1 cut(s)	2385
NdeII	GATC	8 cut(s)	48, 661, 992, 1009, 1052, 1744, 1939, 2080
NmuCI	GTSAC	1 cut(s)	2313
NsiI	ATGCAT	4 cut(s)	586, 655, 1838, 2200
NspI	RCATGY	4 cut(s)	584, 653, 805, 2202
PaeI	GCATGC	1 cut(s)	653
PciI	ACATGT	1 cut(s)	801
PciSI	GCTCTTC	1 cut(s)	2556
PctI	GAATGC	2 cut(s)	79, 764
PdmI	GAANNNNTTC	2 cut(s)	598, 2565
PfeI	GAWTC	4 cut(s)	39, 503, 1159, 1669
PleI	GAGTC	1 cut(s)	973
PmaCI	CACGTG	1 cut(s)	179
PmlI	CACGTG	1 cut(s)	179
PpsI	GAGTC	1 cut(s)	973
Ppu21I	YACGTR	2 cut(s)	179, 2432
PpuMI	RGGWCCY	3 cut(s)	1903, 2011, 2378
PscI	ACATGT	1 cut(s)	801
PshBI	ATTAAT	1 cut(s)	1706
PsiI	TTATAA	2 cut(s)	1349, 2601
Psp5II	RGGWCCY	3 cut(s)	1903, 2011, 2378
Psp6I	CCWGG	3 cut(s)	514, 1217, 2583
PspCI	CACGTG	1 cut(s)	179
PspGI	CCWGG	3 cut(s)	514, 1217, 2583
PspPI	GGNCC	6 cut(s)	345, 1903, 2011, 2205, 2378, 2632
PspPPI	RGGWCCY	3 cut(s)	1903, 2011, 2378
PsrI	GAACNNNNNNTAC	2 cut(s)	2085, 2117
PstNI	CAGNNNCTG	1 cut(s)	1652
PsuI	RGATCY	4 cut(s)	992, 1052, 1744, 2080
PvuII	CAGCTG	1 cut(s)	641
RsaI	GTAC	3 cut(s)	337, 513, 1893
RsaNI	GTAC	3 cut(s)	336, 512, 1892
RseI	CAYNNNNRTG	1 cut(s)	1296
SapI	GCTCTTC	1 cut(s)	2556
SaqAI	TTAA	8 cut(s)	240, 561, 782, 899, 1316, 1457, 1706, 2226
Sau3AI	GATC	8 cut(s)	48, 661, 992, 1009, 1052, 1744, 1939, 2080
Sau96I	GGNCC	6 cut(s)	345, 1903, 2011, 2205, 2378, 2632
SchI	GAGTC	1 cut(s)	974
ScrFI	CCNGG	5 cut(s)	516, 1219, 1798, 1900, 2585
SduI	GDGCHC	1 cut(s)	1505
SexAI	ACCWGGT	1 cut(s)	2583
SfiI	GGCCNNNNNGGCC	1 cut(s)	1196
SinI	GGWCC	5 cut(s)	345, 1903, 2011, 2378, 2632
SmiMI	CAYNNNNRTG	1 cut(s)	1296
SmlI	CTYRAG	3 cut(s)	492, 560, 898
SmoI	CTYRAG	3 cut(s)	492, 560, 898
SphI	GCATGC	1 cut(s)	653
SspI	AATATT	1 cut(s)	1084
SspMI	CTAG	5 cut(s)	371, 1469, 1520, 1643, 1760
StyD4I	CCNGG	5 cut(s)	514, 1217, 1796, 1898, 2583
StyI	CCWWGG	7 cut(s)	17, 27, 258, 603, 657, 2341, 2649
TaaI	ACNGT	7 cut(s)	340, 888, 1007, 1891, 2179, 2263, 2330
TaiI	ACGT	4 cut(s)	181, 532, 543, 2434
TaqI	TCGA	2 cut(s)	205, 1128
TaqII	GACCGA	1 cut(s)	336
TatI	WGTACW	1 cut(s)	335
TauI	GCSGC	2 cut(s)	8, 47
TfiI	GAWTC	4 cut(s)	39, 503, 1159, 1669
Tru1I	TTAA	8 cut(s)	240, 561, 782, 899, 1316, 1457, 1706, 2226
Tru9I	TTAA	8 cut(s)	240, 561, 782, 899, 1316, 1457, 1706, 2226
TscAI	CASTG	9 cut(s)	201, 829, 1120, 1210, 1282, 1978, 2335, 2642, 2667
TseFI	GTSAC	1 cut(s)	2313
Tsp45I	GTSAC	1 cut(s)	2313
TspGWI	ACGGA	2 cut(s)	746, 2048
TspRI	CASTG	9 cut(s)	201, 829, 1120, 1210, 1282, 1978, 2335, 2642, 2667
Vha464I	CTTAAG	2 cut(s)	560, 898
VpaK11BI	GGWCC	5 cut(s)	345, 1903, 2011, 2378, 2632
VspI	ATTAAT	1 cut(s)	1706
XagI	CCTNNNNNAGG	1 cut(s)	306
XapI	RAATTY	9 cut(s)	378, 854, 1099, 1689, 1732, 1919, 2126, 2241, 2506
XbaI	TCTAGA	1 cut(s)	1519
XceI	RCATGY	4 cut(s)	584, 653, 805, 2202
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2591
XmiI	GTMKAC	1 cut(s)	1623
XmnI	GAANNNNTTC	2 cut(s)	598, 2565
XspI	CTAG	5 cut(s)	371, 1469, 1520, 1643, 1760
Zsp2I	ATGCAT	4 cut(s)	586, 655, 1838, 2200

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.