Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0007704

Physical Location & Seq

Genomic Coordinates Forward (+)

24727211 .. 24740342

Gene Structure 13132 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1125000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2697 bp

ATGGCGGCAACCGGCCCGTGGCAGCCCAAGGAAGAAGGGTTCGCGGCGATCTGCGGGCTGCTGGAGCAGCAGATTTCGCATTCGGCTTCCTCCGCGGACAAGTCTCAGATTTGGCAGCAGCTCCAGCACTACTCTCAGTTCCCCGATTTCAACAACTACCTTGCGTTCATTCTCGCACGTGCCGAGGGTAAATCGGTTGAGGTTCGACAGGCAGCGGGATTGCTTTTGAAGAATAATCTTAAAAATGCTTATACTTCCATGGCTCCTGCATACCAGCAATATATAAAATCGGAATTGTTGCCTTGCTTAGGAGTAGCAGACAGACACGTCAGATCTACAGTTGGGACCATTGTAACTGTTGTTGTTCAGCTAGAGGGAATTTTGGGGTGGCCTGAACTATTGCAAGCTCTTGTAAATTGTTTAGACAGTAATGACCTAAATCACATGGAAGGTGCTATGGACGCATTATCCAAGATTTGTGAGGATATACCTCAACTGCTTGATTCAGATGTACCTGGATTGCCTGAACGTCCCATCAACGTTTTCTTTCCAAGATTACTTAAGTTTTTCCAATCGCCACATGCATCGCTTAGAAAGCTTTCCTTGGGTTCTGTAAATCAATATATTATGTTGATGCCAGCTGCTCTGCATGCATCTATGGATCAATATCTTCAAGGTTTGTTTATCCTTGCTAATGACCCGTCTTCAGAAGTGAGGAAATTGGTTAGTGCCGCATTTGTCCAACTAATTGAAGTCCGTCCGGCATTCTTGGAGCCACATTTGAGGAATGTAATTGAATACATGTTGCAAGTCAACAACGATACTGAGGAAGAAGTGGCTCTTGAAGCCTGTGAATTTTGGTCTGCATATTGTGAAGCTCAGTTACCGCCTGAGAACTTAATAGAGTTTTTGCCACGCCTAATTCCGATTTTGTTATCAAACATGGTTTATTCTGATGATGATGAGTCCCTTGCTGATGCTGAGGAGGATGGATCTGTCCCAGACCGTGATCAGGATATCAAACCTCGGTTTCATTCATCACGGCTTCATGGATCTGATAATGTGGAAGATGATGATGATGATATTGTTGGTATATGGAATTTACGCAAATGCAGTGCAGCAGCTCTCGATATCCTGTCAAATGTGTTTGGCGATGAGATTCTCCCAACGTTGATGCCGTTTGTTCAGGCCAAGTTGGCCACCAGTGATGATGAAACCTGGAAAGGAAGGGAAGCTGCTGTTTTGGCTCTAGGTGCCATAGCTGAAGGTTGCATTAGTGGTCTTTATCCTCATTTGAATGAGATTATTGCATTTTTAATCCCCCTTTTGGACGATAAGTTTCCACTTATAAGGAGTATTTCTTGTTGGACACTTTCTCGTTTCAGCAAGTTCATTGTTCAGGGTGCCGGACATCAAAAAGGTTATGAGCAATTTGACAAGGTTCTTGTGGGTCTATTAAGAAGAATATTAGATAATAACAAGCGGGTGCAAGAAGCTGCTTGCTCTGCTTTTGCAACACTAGAAGAGGAGGCAGCAGAAGAGTTGGCACCACGCTTGGAAATGATTTTACAACACCTTATGTGTGCTTTTGGGAAATATCAGAGGAGAAACCTCAGAATTGTATACGATGCTATTGGAACTCTAGCAGATGCTGTCGGGGGAGAGTTGAATCAGCCCACTTATCTTGAAATTCTGATGCCACCATTAATTGCAAAGTGGCAACAGCTTTCAAATTCAGATAAAGATCTGTTTCCATTGCTAGAGTGCTTTACTTCTATATCACAGGCATTGGGTGCTGGGTTCTCTCAGTTTGCTGAACCTGTATTTCAGAGATGCATAAGCATCATCGAGTCCCAACAACTCGCAAAGGTTCAGGCAAAGGTCAATCCTGTACCTTCAGGGGTCCCGTATGATAAAGAATTTATTGTTTGCTCTCTTGATCTGCTCTCTGGACTTGCAGAAGGTCTTGGCAGTGGGATAGAGAGTTTGGTTTCACAAAGCAATTTGAGGGACCTGCTTTTGCAATGTTGCACGGATGATGCTTCGGATGTCCGGCAGAGTGGATTTGCACTACTTGGGGATCTTGCAAGAGTATGCCCAGTTCACTTGCAACCTCGTTTGCCAGAATTTCTTGATATCGCAGCCAAGCAACTGAATACTCCTAAGCTGCAAGAAACTGTTTCAGTTGCAAACAATGCATGTTGGGCCATTGGAGAGTTAGCAGTTAAGGTTCGTCAAGAAATTTCTCCAATTGTTCTGACAGTTATCTCTTGCTTAGTTCAAATCCTTCAACACGCAGAGGAGCTGAACAAGTCACTAATTGAAAACAGTGCTATCACCCTTGGGAGGCTTGCGTGGGTCTGTCCAGAGCTTGTGGCTCCCCATATGGAGCATTTCATGCAACGATGGTGCATTGCTTTGTCAATGATACGTGATGATGTAGAAAAGGAGGATGCCTTCCGAGGTCTCTGTGCTTTGGTTAGAACCAATCCATCCGGAGCATTGAATTCACTCATTTATCTGTGCAATGCTATTGCAAGTTGGCATGAAATACGGAGCGAAGAGCTTCATAATGAAGTTCACCAGGTGTTGCATGGTTATAAGCAGATGCTTGCGAATGGAGGAGCTTGGGACCAATGTATGTCTGCCTTGGTGCCACCAGTGAAGGAAAAGCTTTCAAAAATATACCAAGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000060 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0017038 GO:0031090 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0033036 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0042277 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070013 GO:0070727 GO:0071702 GO:0071705 GO:0072594

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HEAT HEAT_EZ IBN_N Ribosomal_S17

Protein Analysis

899

Amino Acids

99.67

Weight (kDa)

4.87

Isoelectric Point (pI)

49.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IBN_N	PF03810	38 - 104	2.8e-12	Importin-beta N-terminal domain
TPR_IPO5	PF25780	122 - 266	2.5e-06	IPO5-like, TPR repeats
HEAT_EZ	PF13513	410 - 461	6.1e-14	HEAT-like repeat
TPR_IMB1	PF25574	485 - 700	2.8e-07	Importin subunit beta-1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007704)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16950 AT2G16950
fragaria_vesca	FvH4_2g15650 FvH4_2g15650 FvH4_2g15650 FvH4_2g15650 FvH4_2g15650 FvH4_2g15650
malus_domestica	MD05G1125000.v1.1 MD10G1127800.v1.1
prunus_persica	Prupe.8G169000_v2.0.a1 Prupe.8G169000_v2.0.a1 Prupe.8G169000_v2.0.a1
pyrus_communis	pycom05g11910 pycom10g10950
rosa_chinensis	RchiOBHm_Chr6g0278851
rosa_laevigata	RLG00000013189
rosa_multiflora	Rmu_sc0004380.1_g000009 Rmu_sc0024310.1_g000003
rosa_roxburghii	Rroxscaffold_7G00189590
rosa_rugosa	Rorug06G0119700
rosa_samantha	Rh6AG228200 Rh6BG233800
rosa_wichuraiana	Rw6G020020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1349, 2601
AasI	GACNNNNNNGTC	1 cut(s)	326
Acc36I	ACCTGC	1 cut(s)	2022
AccB1I	GGYRCC	4 cut(s)	1253, 1403, 1546, 2653
AccI	GTMKAC	1 cut(s)	1623
AccII	CGCG	2 cut(s)	44, 95
AccIII	TCCGGA	1 cut(s)	2495
AciI	CCGC	9 cut(s)	5, 44, 54, 93, 95, 215, 732, 887, 1483
AclI	AACGTT	2 cut(s)	540, 1169
AclWI	GGATC	4 cut(s)	669, 1000, 1060, 2088
AcoI	YGGCCR	1 cut(s)	1197
AcsI	RAATTY	9 cut(s)	378, 854, 1099, 1689, 1732, 1919, 2126, 2241, 2506
AcuI	CTGAAG	3 cut(s)	690, 1284, 1881
AcvI	CACGTG	1 cut(s)	179
AdeI	CACNNNGTG	1 cut(s)	2587
AfaI	GTAC	2 cut(s)	513, 1893
AfiI	CCNNNNNNNGG	5 cut(s)	18, 308, 1012, 1327, 2346
AflII	CTTAAG	1 cut(s)	560
AflIII	ACRYGT	2 cut(s)	325, 801
AjiI	CACGTC	1 cut(s)	328
AjnI	CCWGG	3 cut(s)	514, 1217, 2583
AloI	GAACNNNNNNTCC	2 cut(s)	23, 55
Alw26I	GTCTC	2 cut(s)	108, 2471
AlwI	GGATC	4 cut(s)	669, 1000, 1060, 2088
AlwNI	CAGNNNCTG	1 cut(s)	1652
Aor13HI	TCCGGA	1 cut(s)	2495
AoxI	GGCC	5 cut(s)	13, 389, 1188, 1197, 2205
ApoI	RAATTY	9 cut(s)	378, 854, 1099, 1689, 1732, 1919, 2126, 2241, 2506
AseI	ATTAAT	1 cut(s)	1706
Asp700I	GAANNNNTTC	2 cut(s)	598, 2565
AspS9I	GGNCC	6 cut(s)	14, 345, 1903, 2011, 2205, 2632
AsuHPI	GGTGA	2 cut(s)	2329, 2573
AvaII	GGWCC	4 cut(s)	345, 1903, 2011, 2632
BalI	TGGCCA	1 cut(s)	1199
BanI	GGYRCC	4 cut(s)	1253, 1403, 1546, 2653
BarI	GAAGNNNNNNTAC	2 cut(s)	867, 899
BbrPI	CACGTG	1 cut(s)	179
BbsI	GAAGAC	1 cut(s)	696
BbvCI	CCTCAGC	1 cut(s)	981
BccI	CCATC	4 cut(s)	542, 983, 2400, 2500
BceAI	ACGGC	2 cut(s)	1058, 1162
BciT130I	CCWGG	3 cut(s)	516, 1219, 2585
BclI	TGATCA	1 cut(s)	1009
BcoDI	GTCTC	2 cut(s)	108, 2471
BfaI	CTAG	5 cut(s)	371, 1250, 1520, 1643, 1760
BfmI	CTRYAG	1 cut(s)	336
BfrI	CTTAAG	1 cut(s)	560
BfuAI	ACCTGC	1 cut(s)	2022
BglI	GCCNNNNNGGC	1 cut(s)	1196
BglII	AGATCT	2 cut(s)	332, 1744
Bme1390I	CCNGG	3 cut(s)	516, 1219, 2585
Bme18I	GGWCC	4 cut(s)	345, 1903, 2011, 2632
BmgBI	CACGTC	1 cut(s)	328
BmgT120I	GGNCC	6 cut(s)	14, 345, 1903, 2011, 2205, 2632
BmrFI	CCNGG	3 cut(s)	516, 1219, 2585
BmrI	ACTGGG	1 cut(s)	2093
BmuI	ACTGGG	1 cut(s)	2093
BpiI	GAAGAC	1 cut(s)	696
BpmI	CTGGAG	2 cut(s)	83, 107
Bpu10I	CCTNAGC	3 cut(s)	307, 981, 2163
BsaAI	YACGTR	2 cut(s)	179, 2432
BsaBI	GATNNNNATC	2 cut(s)	666, 1743
BsaI	GGTCTC	1 cut(s)	2471
BsaWI	WCCGGW	1 cut(s)	2495
Bsc4I	CCNNNNNNNGG	5 cut(s)	18, 308, 1012, 1327, 2346
Bse118I	RCCGGY	1 cut(s)	11
Bse1I	ACTGG	3 cut(s)	1203, 2099, 2660
Bse3DI	GCAATG	4 cut(s)	1754, 2030, 2412, 2533
Bse8I	GATNNNNATC	2 cut(s)	666, 1743
BseAI	TCCGGA	1 cut(s)	2495
BseBI	CCWGG	3 cut(s)	516, 1219, 2585
BseGI	GGATG	5 cut(s)	994, 2041, 2053, 2458, 2492
BseJI	GATNNNNATC	2 cut(s)	666, 1743
BseLI	CCNNNNNNNGG	5 cut(s)	18, 308, 1012, 1327, 2346
BseMI	GCAATG	4 cut(s)	1754, 2030, 2412, 2533
BseMII	CTCAG	8 cut(s)	119, 149, 816, 882, 893, 972, 1627, 1820
BseNI	ACTGG	3 cut(s)	1203, 2099, 2660
BseRI	GAGGAG	5 cut(s)	998, 1541, 1618, 2315, 2637
BseYI	CCCAGC	1 cut(s)	1796
BsgI	GTGCAG	1 cut(s)	1137
Bsh1236I	CGCG	2 cut(s)	44, 95
BshFI	GGCC	5 cut(s)	15, 391, 1190, 1199, 2207
BshNI	GGYRCC	4 cut(s)	1253, 1403, 1546, 2653
BsiSI	CCGG	5 cut(s)	12, 761, 1407, 2053, 2496
BslFI	GGGAC	8 cut(s)	358, 516, 952, 983, 1837, 1889, 2024, 2645
BslI	CCNNNNNNNGG	5 cut(s)	18, 308, 1012, 1327, 2346
BsmAI	GTCTC	2 cut(s)	108, 2471
BsmFI	GGGAC	8 cut(s)	358, 516, 952, 983, 1837, 1889, 2024, 2645
BsmI	GAATGC	2 cut(s)	79, 764
BsnI	GGCC	5 cut(s)	15, 391, 1190, 1199, 2207
Bso31I	GGTCTC	1 cut(s)	2471
Bsp13I	TCCGGA	1 cut(s)	2495
Bsp143I	GATC	9 cut(s)	48, 332, 661, 992, 1009, 1052, 1744, 1939, 2080
Bsp19I	CCATGG	1 cut(s)	258
BspACI	CCGC	9 cut(s)	5, 44, 54, 93, 95, 215, 732, 887, 1483
BspANI	GGCC	5 cut(s)	15, 391, 1190, 1199, 2207
BspCNI	CTCAG	8 cut(s)	118, 148, 817, 883, 892, 973, 1626, 1819
BspEI	TCCGGA	1 cut(s)	2495
BspFNI	CGCG	2 cut(s)	44, 95
BspMI	ACCTGC	1 cut(s)	2022
BspPI	GGATC	4 cut(s)	669, 1000, 1060, 2088
BspQI	GCTCTTC	1 cut(s)	2556
BspT107I	GGYRCC	4 cut(s)	1253, 1403, 1546, 2653
BspTI	CTTAAG	1 cut(s)	560
BspTNI	GGTCTC	1 cut(s)	2471
BsrDI	GCAATG	4 cut(s)	1754, 2030, 2412, 2533
BsrFI	RCCGGY	1 cut(s)	11
BsrI	ACTGG	3 cut(s)	1203, 2099, 2660
BssAI	RCCGGY	1 cut(s)	11
BssMI	GATC	9 cut(s)	48, 332, 661, 992, 1009, 1052, 1744, 1939, 2080
BssNAI	GTATAC	1 cut(s)	1624
BssT1I	CCWWGG	5 cut(s)	27, 258, 603, 2341, 2649
Bst1107I	GTATAC	1 cut(s)	1624
Bst2UI	CCWGG	3 cut(s)	516, 1219, 2585
Bst4CI	ACNGT	7 cut(s)	340, 358, 428, 1007, 2179, 2263, 2330
Bst6I	CTCTTC	3 cut(s)	1518, 1533, 2556
BstAFI	CTTAAG	1 cut(s)	560
BstAPI	GCANNNNNTGC	3 cut(s)	1793, 2195, 2398
BstBAI	YACGTR	2 cut(s)	179, 2432
BstC8I	GCNNGC	7 cut(s)	56, 405, 639, 651, 1501, 2352, 2613
BstDSI	CCRYGG	3 cut(s)	17, 93, 258
BstENI	CCTNNNNNAGG	1 cut(s)	306
BstF5I	GGATG	5 cut(s)	994, 2041, 2053, 2458, 2492
BstFNI	CGCG	2 cut(s)	44, 95
BstKTI	GATC	9 cut(s)	51, 335, 664, 995, 1012, 1055, 1747, 1942, 2083
BstMAI	GTCTC	2 cut(s)	108, 2471
BstMBI	GATC	9 cut(s)	48, 332, 661, 992, 1009, 1052, 1744, 1939, 2080
BstNI	CCWGG	3 cut(s)	516, 1219, 2585
BstNSI	RCATGY	4 cut(s)	584, 653, 805, 2202
BstSCI	CCNGG	3 cut(s)	514, 1217, 2583
BstSFI	CTRYAG	1 cut(s)	336
BstUI	CGCG	2 cut(s)	44, 95
BstV2I	GAAGAC	1 cut(s)	696
BstX2I	RGATCY	5 cut(s)	332, 992, 1052, 1744, 2080
BstYI	RGATCY	5 cut(s)	332, 992, 1052, 1744, 2080
BstZ17I	GTATAC	1 cut(s)	1624
BsuRI	GGCC	5 cut(s)	15, 391, 1190, 1199, 2207
BtgI	CCRYGG	3 cut(s)	17, 93, 258
BtgZI	GCGATG	2 cut(s)	570, 1167
BtrI	CACGTC	1 cut(s)	328
BtsCI	GGATG	5 cut(s)	994, 2041, 2053, 2458, 2492
BtsI	GCAGTG	2 cut(s)	1120, 1978
BtsIMutI	CAGTG	5 cut(s)	1120, 1210, 1978, 2335, 2667
BveI	ACCTGC	1 cut(s)	2022
Cac8I	GCNNGC	7 cut(s)	56, 405, 639, 651, 1501, 2352, 2613
CaiI	CAGNNNCTG	1 cut(s)	1652
Cfr10I	RCCGGY	1 cut(s)	11
Cfr13I	GGNCC	6 cut(s)	14, 345, 1903, 2011, 2205, 2632
Cfr42I	CCGCGG	1 cut(s)	96
CseI	GACGC	1 cut(s)	470
CsiI	ACCWGGT	1 cut(s)	2583
Csp6I	GTAC	2 cut(s)	512, 1892
CspCI	CAANNNNNGTGG	2 cut(s)	1666, 1701
CviQI	GTAC	2 cut(s)	512, 1892
DpnI	GATC	9 cut(s)	50, 334, 663, 994, 1011, 1054, 1746, 1941, 2082
DpnII	GATC	9 cut(s)	48, 332, 661, 992, 1009, 1052, 1744, 1939, 2080
DraIII	CACNNNGTG	1 cut(s)	2587
DrdI	GACNNNNNNGTC	1 cut(s)	326
DseDI	GACNNNNNNGTC	1 cut(s)	326
EaeI	YGGCCR	1 cut(s)	1197
Eam1104I	CTCTTC	3 cut(s)	1518, 1533, 2556
EarI	CTCTTC	3 cut(s)	1518, 1533, 2556
Eco130I	CCWWGG	5 cut(s)	27, 258, 603, 2341, 2649
Eco31I	GGTCTC	1 cut(s)	2471
Eco32I	GATATC	3 cut(s)	1018, 1132, 2137
Eco47I	GGWCC	4 cut(s)	345, 1903, 2011, 2632
Eco57I	CTGAAG	3 cut(s)	690, 1284, 1881
Eco72I	CACGTG	1 cut(s)	179
EcoNI	CCTNNNNNAGG	1 cut(s)	306
EcoO109I	RGGNCCY	2 cut(s)	1903, 2011
EcoRI	GAATTC	1 cut(s)	2506
EcoRII	CCWGG	3 cut(s)	514, 1217, 2583
EcoRV	GATATC	3 cut(s)	1018, 1132, 2137
EcoT14I	CCWWGG	5 cut(s)	27, 258, 603, 2341, 2649
EcoT22I	ATGCAT	4 cut(s)	586, 655, 1838, 2200
ErhI	CCWWGG	5 cut(s)	27, 258, 603, 2341, 2649
FalI	AAGNNNNNCTT	4 cut(s)	587, 619, 825, 857
FaqI	GGGAC	8 cut(s)	358, 516, 952, 983, 1837, 1889, 2024, 2645
FauI	CCCGC	3 cut(s)	47, 208, 1476
FauNDI	CATATG	1 cut(s)	2385
FbaI	TGATCA	1 cut(s)	1009
FblI	GTMKAC	1 cut(s)	1623
FokI	GGATG	5 cut(s)	1001, 2048, 2060, 2465, 2479
FspBI	CTAG	5 cut(s)	371, 1250, 1520, 1643, 1760
GsaI	CCCAGC	1 cut(s)	1800
GsuI	CTGGAG	2 cut(s)	83, 107
HaeIII	GGCC	5 cut(s)	15, 391, 1190, 1199, 2207
HapII	CCGG	5 cut(s)	12, 761, 1407, 2053, 2496
HgaI	GACGC	1 cut(s)	470
HincII	GTYRAC	1 cut(s)	814
HindII	GTYRAC	1 cut(s)	814
HindIII	AAGCTT	2 cut(s)	596, 2672
HinfI	GANTC	5 cut(s)	503, 965, 1159, 1669, 1850
HpaII	CCGG	5 cut(s)	12, 761, 1407, 2053, 2496
HphI	GGTGA	2 cut(s)	2329, 2573
Hpy166II	GTNNAC	4 cut(s)	814, 1624, 2104, 2581
Hpy8I	GTNNAC	4 cut(s)	814, 1624, 2104, 2581
HpyAV	CCTTC	9 cut(s)	29, 443, 1221, 1259, 1905, 1955, 2297, 2467, 2659
HpyCH4III	ACNGT	7 cut(s)	340, 358, 428, 1007, 2179, 2263, 2330
HpyCH4IV	ACGT	6 cut(s)	178, 327, 529, 540, 1169, 2431
HpySE526I	ACGT	6 cut(s)	178, 327, 529, 540, 1169, 2431
KflI	GGGWCCC	1 cut(s)	1903
Kpn2I	TCCGGA	1 cut(s)	2495
Ksp22I	TGATCA	1 cut(s)	1009
KspI	CCGCGG	1 cut(s)	96
Kzo9I	GATC	9 cut(s)	48, 332, 661, 992, 1009, 1052, 1744, 1939, 2080
LguI	GCTCTTC	1 cut(s)	2556
MabI	ACCWGGT	1 cut(s)	2583
MaeI	CTAG	5 cut(s)	371, 1250, 1520, 1643, 1760
MaeII	ACGT	6 cut(s)	178, 327, 529, 540, 1169, 2431
MaeIII	GTNAC	3 cut(s)	352, 882, 2313
MalI	GATC	9 cut(s)	50, 334, 663, 994, 1011, 1054, 1746, 1941, 2082
MboI	GATC	9 cut(s)	48, 332, 661, 992, 1009, 1052, 1744, 1939, 2080
MfeI	CAATTG	1 cut(s)	2250
MflI	RGATCY	5 cut(s)	332, 992, 1052, 1744, 2080
MlsI	TGGCCA	1 cut(s)	1199
MluNI	TGGCCA	1 cut(s)	1199
MlyI	GAGTC	2 cut(s)	974, 1859
MmeI	TCCRAC	2 cut(s)	766, 1346
Mox20I	TGGCCA	1 cut(s)	1199
Mph1103I	ATGCAT	4 cut(s)	586, 655, 1838, 2200
MroI	TCCGGA	1 cut(s)	2495
MroXI	GAANNNNTTC	2 cut(s)	598, 2565
MscI	TGGCCA	1 cut(s)	1199
MseI	TTAA	7 cut(s)	240, 561, 899, 1316, 1457, 1706, 2226
MslI	CAYNNNNRTG	1 cut(s)	1296
Msp20I	TGGCCA	1 cut(s)	1199
MspA1I	CMGCKG	3 cut(s)	95, 215, 641
MspCI	CTTAAG	1 cut(s)	560
MspI	CCGG	5 cut(s)	12, 761, 1407, 2053, 2496
MspR9I	CCNGG	3 cut(s)	516, 1219, 2585
MunI	CAATTG	1 cut(s)	2250
Mva1269I	GAATGC	2 cut(s)	79, 764
MvaI	CCWGG	3 cut(s)	516, 1219, 2585
MvnI	CGCG	2 cut(s)	44, 95
NcoI	CCATGG	1 cut(s)	258
NdeI	CATATG	1 cut(s)	2385
NdeII	GATC	9 cut(s)	48, 332, 661, 992, 1009, 1052, 1744, 1939, 2080
NmeAIII	GCCGAG	1 cut(s)	208
NmuCI	GTSAC	1 cut(s)	2313
NsiI	ATGCAT	4 cut(s)	586, 655, 1838, 2200
NspI	RCATGY	4 cut(s)	584, 653, 805, 2202
PaeI	GCATGC	1 cut(s)	653
PciI	ACATGT	1 cut(s)	801
PciSI	GCTCTTC	1 cut(s)	2556
PcsI	WCGNNNNNNNCGW	2 cut(s)	180, 1175
PctI	GAATGC	2 cut(s)	79, 764
PdmI	GAANNNNTTC	2 cut(s)	598, 2565
PfeI	GAWTC	3 cut(s)	503, 1159, 1669
PleI	GAGTC	2 cut(s)	973, 1858
PmaCI	CACGTG	1 cut(s)	179
PmlI	CACGTG	1 cut(s)	179
PpsI	GAGTC	2 cut(s)	973, 1858
Ppu21I	YACGTR	2 cut(s)	179, 2432
PpuMI	RGGWCCY	2 cut(s)	1903, 2011
PscI	ACATGT	1 cut(s)	801
PshBI	ATTAAT	1 cut(s)	1706
PsiI	TTATAA	2 cut(s)	1349, 2601
Psp1406I	AACGTT	2 cut(s)	540, 1169
Psp5II	RGGWCCY	2 cut(s)	1903, 2011
Psp6I	CCWGG	3 cut(s)	514, 1217, 2583
PspCI	CACGTG	1 cut(s)	179
PspFI	CCCAGC	1 cut(s)	1796
PspGI	CCWGG	3 cut(s)	514, 1217, 2583
PspPI	GGNCC	6 cut(s)	14, 345, 1903, 2011, 2205, 2632
PspPPI	RGGWCCY	2 cut(s)	1903, 2011
PsrI	GAACNNNNNNTAC	2 cut(s)	2085, 2117
PstNI	CAGNNNCTG	1 cut(s)	1652
PsuI	RGATCY	5 cut(s)	332, 992, 1052, 1744, 2080
PvuII	CAGCTG	1 cut(s)	641
RsaI	GTAC	2 cut(s)	513, 1893
RsaNI	GTAC	2 cut(s)	512, 1892
RseI	CAYNNNNRTG	1 cut(s)	1296
SacII	CCGCGG	1 cut(s)	96
SapI	GCTCTTC	1 cut(s)	2556
SaqAI	TTAA	7 cut(s)	240, 561, 899, 1316, 1457, 1706, 2226
Sau3AI	GATC	9 cut(s)	48, 332, 661, 992, 1009, 1052, 1744, 1939, 2080
Sau96I	GGNCC	6 cut(s)	14, 345, 1903, 2011, 2205, 2632
SchI	GAGTC	2 cut(s)	974, 1859
ScrFI	CCNGG	3 cut(s)	516, 1219, 2585
SexAI	ACCWGGT	1 cut(s)	2583
SfcI	CTRYAG	1 cut(s)	336
SfiI	GGCCNNNNNGGCC	1 cut(s)	1196
Sfr303I	CCGCGG	1 cut(s)	96
SgrBI	CCGCGG	1 cut(s)	96
SinI	GGWCC	4 cut(s)	345, 1903, 2011, 2632
SmiMI	CAYNNNNRTG	1 cut(s)	1296
SmlI	CTYRAG	1 cut(s)	560
SmoI	CTYRAG	1 cut(s)	560
SphI	GCATGC	1 cut(s)	653
SsiI	CCGC	9 cut(s)	5, 44, 54, 93, 95, 215, 732, 887, 1483
SspI	AATATT	1 cut(s)	1467
SspMI	CTAG	5 cut(s)	371, 1250, 1520, 1643, 1760
StyD4I	CCNGG	3 cut(s)	514, 1217, 2583
StyI	CCWWGG	5 cut(s)	27, 258, 603, 2341, 2649
TaaI	ACNGT	7 cut(s)	340, 358, 428, 1007, 2179, 2263, 2330
TaiI	ACGT	6 cut(s)	181, 330, 532, 543, 1172, 2434
TaqI	TCGA	3 cut(s)	205, 1128, 1848
TauI	GCSGC	3 cut(s)	8, 47, 734
TfiI	GAWTC	3 cut(s)	503, 1159, 1669
Tru1I	TTAA	7 cut(s)	240, 561, 899, 1316, 1457, 1706, 2226
Tru9I	TTAA	7 cut(s)	240, 561, 899, 1316, 1457, 1706, 2226
TscAI	CASTG	5 cut(s)	1120, 1210, 1978, 2335, 2667
TseFI	GTSAC	1 cut(s)	2313
Tsp45I	GTSAC	1 cut(s)	2313
TspGWI	ACGGA	3 cut(s)	746, 2048, 2569
TspRI	CASTG	5 cut(s)	1120, 1210, 1978, 2335, 2667
Vha464I	CTTAAG	1 cut(s)	560
VpaK11BI	GGWCC	4 cut(s)	345, 1903, 2011, 2632
VspI	ATTAAT	1 cut(s)	1706
XagI	CCTNNNNNAGG	1 cut(s)	306
XapI	RAATTY	9 cut(s)	378, 854, 1099, 1689, 1732, 1919, 2126, 2241, 2506
XceI	RCATGY	4 cut(s)	584, 653, 805, 2202
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2591
XmiI	GTMKAC	1 cut(s)	1623
XmnI	GAANNNNTTC	2 cut(s)	598, 2565
XspI	CTAG	5 cut(s)	371, 1250, 1520, 1643, 1760
Zsp2I	ATGCAT	4 cut(s)	586, 655, 1838, 2200

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.