AT2G18520

repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0014081
Physical Location & Seq
Reverse (-)
8033832 .. 8035412
1581 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G18520.1

Sequence Viewer

Length: 1257 bp
ATGACGTCATCTCGCCTCTATCTCCGTTTCCTCCGCCGCTTCTCCACCGCCACCGGTATAGATTCTCAAACCACTGCTTACCCCGGCGCAATCACTATGTCCAAAGCTAAATCCAAACTCCGCAAAGTACAAGATCCAGACAAAGCCTTAGCGATTTACAAATCCGTCTCCAACAATTCCACATCTCCACTCTCCTCTCGTTACGCAATGGAGCTTACAGTCCAACGTCTCGCTAAATCTCAACGCTTCTCCGACATAGAAGCTCTAATTGAGTCTCACAAAAACAACCCAAAGATCAAAACAGAGACTTTTCTCTCTACACTTATCAGATCTTATGGACGAGCTTCAATGTTTGATCATGCTATGAAGATGTTCGAGGAGATGGATAAGCTTGGCACACCCAGAACCGTTGTTTCCTTCAATGCCTTACTCGCTGCTTGTCTTCATTCTGATTTGTTTGAAAGAGTCCCCCAACTGTTCGACGAATTTCCTCAGAGGTATAATAATATCACTCCTGATAAAATCTCTTACGGTATGTTGATTAAGTCGTATTGTGACTCTGGTAAGCCTGAAAAAGCTATGGAGATTATGAGAGATATGGAGGTTAAAGGTGTGGAAGTTACTATCATTGCTTTCACTACCATTTTAGGATCTTTGTATAAGAATGGTTTAGTCGATGAAGCGGAGAGCTTGTGGATTGAGATGGTGAACAAAGGTTGTGACTTGGATAACACAGTTTATAATGTTAGACTTATGAATGCTGCGAAGGAAAGTCCCGAGAGAGTTAAGGAATTGATGGAAGAAATGAGTAGTGTAGGATTGAAACCTGATACTGTTAGCTATAACTATCTTATGACTGCGTACTGCGTGAAAGGAATGATGAGTGAAGCCAAGAAAGTGTATGAAGGGCTTGAGCAACCAAACGCAGCGACGTTCAGGACATTGATATTTCATCTGTGTATAAACGGGTTGTATGATCAAGGTTTAACGGTGTTTAAGAAGAGCGCGATAGTGCACAAGATTCCGGATTTCAAAACTTGTAAGCATTTGACTGAAGGATTGGTGAAGAATAACAGAATGGAAGATGCAAGAGGAGTGGCCAGGATCGTGAAGAAGAAATTCCCTCCGCGTTTGGTGACCGAGTGGAAGAAACTAGAGGAGAAGCTGGGATTGTATTCAAAGGGTAATGCAGCTGCTGTTTCATCTTCTTCTCAAACTAGAGAAGTGTTAGATCAAGAAAGAGAGGATGATGTATAA

Protein Analysis

418

Amino Acids

47.33

Weight (kDa)

9.01

Isoelectric Point (pI)

37.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 107 - 148 1.4e-06 PPR repeat family
PPR_2 PF13041 136 - 186 1.7e-06 PPR repeat family
PPR_1 PF12854 169 - 201 1.4e-07 PPR repeat
PPR_2 PF13041 172 - 216 1.7e-08 PPR repeat family
PPR PF01535 175 - 205 1.2e-06 PPR repeat
PPR_2 PF13041 208 - 249 1.6e-07 PPR repeat family
PPR PF01535 211 - 240 7.3e-06 PPR repeat
PPR_2 PF13041 276 - 320 6.1e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014081)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18520 AT4G36680
fragaria_vesca FvH4_1g13650
malus_domestica MD02G1151200.v1.1 MD15G1265700.v1.1
prunus_persica Prupe.7G151800_v2.0.a1
pyrus_communis pycom02g11870 pycom15g23140
rosa_chinensis RchiOBHm_Chr2g0102001
rosa_laevigata RLG00000017125
rosa_multiflora Rmu_co8487067.1_g000001
rosa_roxburghii Rroxscaffold_2G00140840
rosa_samantha Rh2AG151900 Rh2BG157300 Rh2CG157300 Rh2DG157400
rosa_wichuraiana Rw2G011860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 741
AatII GACGTC 1 cut(s) 8
AccII CGCG 2 cut(s) 1007, 1129
AccIII TCCGGA 1 cut(s) 1024
AciI CCGC 6 cut(s) 34, 37, 48, 121, 683, 1127
AclWI GGATC 3 cut(s) 128, 658, 1112
AcoI YGGCCR 1 cut(s) 1098
AcsI RAATTY 2 cut(s) 485, 1118
AcuI CTGAAG 1 cut(s) 1074
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 129, 863
AgeI ACCGGT 1 cut(s) 53
AgsI TTSAA 6 cut(s) 348, 421, 461, 823, 1033, 1179
AjnI CCWGG 1 cut(s) 1100
Alw21I GWGCWC 1 cut(s) 1017
Alw26I GTCTC 4 cut(s) 172, 233, 279, 299
Alw44I GTGCAC 1 cut(s) 1013
AlwI GGATC 3 cut(s) 128, 658, 1112
AlwNI CAGNNNCTG 1 cut(s) 1196
Ama87I CYCGRG 1 cut(s) 776
Aor13HI TCCGGA 1 cut(s) 1024
AoxI GGCC 1 cut(s) 1098
ApaLI GTGCAC 1 cut(s) 1013
ApeKI GCWGC 5 cut(s) 434, 761, 926, 1190, 1193
ApoI RAATTY 2 cut(s) 485, 1118
AsiGI ACCGGT 1 cut(s) 53
Asp700I GAANNNNTTC 2 cut(s) 371, 1118
AspLEI GCGC 2 cut(s) 89, 1007
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 3 cut(s) 718, 1075, 1147
AvaI CYCGRG 1 cut(s) 776
BaeGI GKGCMC 1 cut(s) 1017
BalI TGGCCA 1 cut(s) 1100
BbsI GAAGAC 1 cut(s) 434
Bbv12I GWGCWC 1 cut(s) 1017
BbvI GCAGC 5 cut(s) 421, 748, 938, 1180, 1202
BccI CCATC 3 cut(s) 376, 697, 790
BciT130I CCWGG 1 cut(s) 1102
BclI TGATCA 2 cut(s) 355, 976
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 4 cut(s) 172, 233, 279, 299
BfaI CTAG 2 cut(s) 1154, 1218
BglII AGATCT 1 cut(s) 329
BisI GCNGC 6 cut(s) 37, 435, 762, 927, 1191, 1194
BlsI GCNGC 6 cut(s) 38, 436, 763, 928, 1192, 1195
Bme1390I CCNGG 2 cut(s) 84, 1102
BmeT110I CYCGRG 1 cut(s) 776
BmrFI CCNGG 2 cut(s) 84, 1102
BmsI GCATC 1 cut(s) 1075
BpiI GAAGAC 1 cut(s) 434
Bpu10I CCTNAGC 1 cut(s) 148
BpuEI CTTGAG 1 cut(s) 932
BpuMI CCSGG 1 cut(s) 84
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 82
BsaWI WCCGGW 2 cut(s) 53, 1024
BsaXI ACNNNNNCTCC 2 cut(s) 677, 707
Bse118I RCCGGY 1 cut(s) 53
Bse3DI GCAATG 2 cut(s) 213, 627
BseAI TCCGGA 1 cut(s) 1024
BseBI CCWGG 1 cut(s) 1102
BseDI CCNNGG 1 cut(s) 82
BseGI GGATG 1 cut(s) 1252
BseMI GCAATG 2 cut(s) 213, 627
BseMII CTCAG 1 cut(s) 506
BseRI GAGGAG 4 cut(s) 184, 392, 1107, 1172
BseSI GKGCMC 1 cut(s) 1017
BseXI GCAGC 5 cut(s) 421, 748, 938, 1180, 1202
BseYI CCCAGC 1 cut(s) 1165
Bsh1236I CGCG 2 cut(s) 1007, 1129
BshFI GGCC 1 cut(s) 1100
BshTI ACCGGT 1 cut(s) 53
BsiHKAI GWGCWC 1 cut(s) 1017
BsiHKCI CYCGRG 1 cut(s) 776
BsiSI CCGG 3 cut(s) 54, 84, 1025
BslFI GGGAC 2 cut(s) 452, 759
BsmAI GTCTC 4 cut(s) 172, 233, 279, 299
BsmBI CGTCTC 2 cut(s) 172, 233
BsmFI GGGAC 2 cut(s) 452, 759
BsmI GAATGC 1 cut(s) 763
BsnI GGCC 1 cut(s) 1100
BsoBI CYCGRG 1 cut(s) 776
Bsp1286I GDGCHC 1 cut(s) 1017
Bsp13I TCCGGA 1 cut(s) 1024
Bsp143I GATC 8 cut(s) 133, 294, 329, 355, 650, 976, 1104, 1231
BspACI CCGC 6 cut(s) 34, 37, 48, 121, 683, 1127
BspANI GGCC 1 cut(s) 1100
BspCNI CTCAG 1 cut(s) 505
BspEI TCCGGA 1 cut(s) 1024
BspFNI CGCG 2 cut(s) 1007, 1129
BspPI GGATC 3 cut(s) 128, 658, 1112
BspQI GCTCTTC 1 cut(s) 995
BsrDI GCAATG 2 cut(s) 213, 627
BsrFI RCCGGY 1 cut(s) 53
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 1 cut(s) 82
BssMI GATC 8 cut(s) 133, 294, 329, 355, 650, 976, 1104, 1231
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 1 cut(s) 1102
Bst4CI ACNGT 7 cut(s) 220, 409, 477, 533, 736, 835, 991
Bst6I CTCTTC 1 cut(s) 995
BstACI GRCGYC 1 cut(s) 5
BstDEI CTNAG 2 cut(s) 148, 492
BstEII GGTNACC 1 cut(s) 1135
BstF5I GGATG 1 cut(s) 1252
BstFNI CGCG 2 cut(s) 1007, 1129
BstHHI GCGC 2 cut(s) 89, 1007
BstKTI GATC 8 cut(s) 136, 297, 332, 358, 653, 979, 1107, 1234
BstMAI GTCTC 4 cut(s) 172, 233, 279, 299
BstMBI GATC 8 cut(s) 133, 294, 329, 355, 650, 976, 1104, 1231
BstMWI GCNNNNNNNGC 1 cut(s) 431
BstNI CCWGG 1 cut(s) 1102
BstPI GGTNACC 1 cut(s) 1135
BstSCI CCNGG 2 cut(s) 82, 1100
BstSLI GKGCMC 1 cut(s) 1017
BstUI CGCG 2 cut(s) 1007, 1129
BstV1I GCAGC 5 cut(s) 421, 748, 938, 1180, 1202
BstV2I GAAGAC 1 cut(s) 434
BstX2I RGATCY 3 cut(s) 133, 329, 650
BstYI RGATCY 3 cut(s) 133, 329, 650
BsuRI GGCC 1 cut(s) 1100
BtsCI GGATG 1 cut(s) 1252
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 1 cut(s) 72
CaiI CAGNNNCTG 1 cut(s) 1196
CfoI GCGC 2 cut(s) 89, 1007
Cfr10I RCCGGY 1 cut(s) 53
Csp6I GTAC 2 cut(s) 128, 862
CspAI ACCGGT 1 cut(s) 53
CspCI CAANNNNNGTGG 2 cut(s) 1077, 1112
CviAII CATG 1 cut(s) 359
CviQI GTAC 2 cut(s) 128, 862
DdeI CTNAG 2 cut(s) 148, 492
DpnI GATC 8 cut(s) 135, 296, 331, 357, 652, 978, 1106, 1233
DpnII GATC 8 cut(s) 133, 294, 329, 355, 650, 976, 1104, 1231
EaeI YGGCCR 1 cut(s) 1098
Eam1104I CTCTTC 1 cut(s) 995
EarI CTCTTC 1 cut(s) 995
EciI GGCGGA 1 cut(s) 23
Eco57I CTGAAG 1 cut(s) 1074
Eco88I CYCGRG 1 cut(s) 776
Eco91I GGTNACC 1 cut(s) 1135
EcoO65I GGTNACC 1 cut(s) 1135
EcoRII CCWGG 1 cut(s) 1100
Esp3I CGTCTC 2 cut(s) 172, 233
FaeI CATG 1 cut(s) 362
FaqI GGGAC 2 cut(s) 452, 759
FatI CATG 1 cut(s) 358
FbaI TGATCA 2 cut(s) 355, 976
Fnu4HI GCNGC 6 cut(s) 37, 435, 762, 927, 1191, 1194
Fsp4HI GCNGC 6 cut(s) 37, 435, 762, 927, 1191, 1194
FspBI CTAG 2 cut(s) 1154, 1218
GlaI GCGC 2 cut(s) 88, 1006
GluI GCNGC 6 cut(s) 37, 435, 762, 927, 1191, 1194
GsaI CCCAGC 1 cut(s) 1169
HaeIII GGCC 1 cut(s) 1100
HapII CCGG 3 cut(s) 54, 84, 1025
HhaI GCGC 2 cut(s) 89, 1007
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 1 cut(s) 362
Hin6I GCGC 2 cut(s) 87, 1005
HinP1I GCGC 2 cut(s) 87, 1005
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 5 cut(s) 62, 272, 465, 557, 1021
HpaII CCGG 3 cut(s) 54, 84, 1025
HphI GGTGA 3 cut(s) 718, 1075, 1147
Hpy166II GTNNAC 2 cut(s) 709, 1015
Hpy188I TCNGA 4 cut(s) 253, 329, 451, 495
Hpy188III TCNNGA 7 cut(s) 137, 515, 776, 937, 1025, 1108, 1235
Hpy8I GTNNAC 2 cut(s) 709, 1015
Hpy99I CGWCG 2 cut(s) 485, 934
HpyAV CCTTC 4 cut(s) 427, 760, 899, 1049
HpyCH4III ACNGT 7 cut(s) 220, 409, 477, 533, 736, 835, 991
HpyCH4IV ACGT 3 cut(s) 5, 226, 932
HpyCH4V TGCA 3 cut(s) 1015, 1088, 1190
HpyF10VI GCNNNNNNNGC 1 cut(s) 431
HpyF3I CTNAG 2 cut(s) 148, 492
HpySE526I ACGT 3 cut(s) 5, 226, 932
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 1 cut(s) 362
HspAI GCGC 2 cut(s) 87, 1005
Kpn2I TCCGGA 1 cut(s) 1024
Ksp22I TGATCA 2 cut(s) 355, 976
Kzo9I GATC 8 cut(s) 133, 294, 329, 355, 650, 976, 1104, 1231
LguI GCTCTTC 1 cut(s) 995
LmnI GCTCC 1 cut(s) 211
Lsp1109I GCAGC 5 cut(s) 421, 748, 938, 1180, 1202
LweI GCATC 1 cut(s) 1075
MaeI CTAG 2 cut(s) 1154, 1218
MaeII ACGT 3 cut(s) 5, 226, 932
MaeIII GTNAC 5 cut(s) 200, 554, 619, 719, 1135
MalI GATC 8 cut(s) 135, 296, 331, 357, 652, 978, 1106, 1233
MboI GATC 8 cut(s) 133, 294, 329, 355, 650, 976, 1104, 1231
MflI RGATCY 3 cut(s) 133, 329, 650
MhlI GDGCHC 1 cut(s) 1017
MlsI TGGCCA 1 cut(s) 1100
MluCI AATT 5 cut(s) 175, 267, 485, 791, 1118
MluNI TGGCCA 1 cut(s) 1100
MlyI GAGTC 3 cut(s) 281, 474, 551
MmeI TCCRAC 3 cut(s) 195, 247, 276
Mox20I TGGCCA 1 cut(s) 1100
MroI TCCGGA 1 cut(s) 1024
MroXI GAANNNNTTC 2 cut(s) 371, 1118
MscI TGGCCA 1 cut(s) 1100
MseI TTAA 5 cut(s) 543, 606, 786, 986, 996
Msp20I TGGCCA 1 cut(s) 1100
MspA1I CMGCKG 1 cut(s) 1193
MspI CCGG 3 cut(s) 54, 84, 1025
MspR9I CCNGG 2 cut(s) 84, 1102
Mva1269I GAATGC 1 cut(s) 763
MvaI CCWGG 1 cut(s) 1102
MvnI CGCG 2 cut(s) 1007, 1129
MwoI GCNNNNNNNGC 1 cut(s) 431
NciI CCSGG 1 cut(s) 84
NdeII GATC 8 cut(s) 133, 294, 329, 355, 650, 976, 1104, 1231
NlaIII CATG 1 cut(s) 362
NmuCI GTSAC 3 cut(s) 554, 719, 1135
PciSI GCTCTTC 1 cut(s) 995
PctI GAATGC 1 cut(s) 763
PdmI GAANNNNTTC 2 cut(s) 371, 1118
PfeI GAWTC 2 cut(s) 62, 1021
PinAI ACCGGT 1 cut(s) 53
PkrI GCNGC 6 cut(s) 38, 436, 763, 928, 1192, 1195
PleI GAGTC 3 cut(s) 280, 473, 551
PpsI GAGTC 3 cut(s) 280, 473, 551
PsiI TTATAA 1 cut(s) 741
Psp6I CCWGG 1 cut(s) 1100
PspEI GGTNACC 1 cut(s) 1135
PspFI CCCAGC 1 cut(s) 1165
PspGI CCWGG 1 cut(s) 1100
PstNI CAGNNNCTG 1 cut(s) 1196
PsuI RGATCY 3 cut(s) 133, 329, 650
PvuII CAGCTG 1 cut(s) 1193
RsaI GTAC 2 cut(s) 129, 863
RsaNI GTAC 2 cut(s) 128, 862
SapI GCTCTTC 1 cut(s) 995
SaqAI TTAA 5 cut(s) 543, 606, 786, 986, 996
SatI GCNGC 6 cut(s) 37, 435, 762, 927, 1191, 1194
Sau3AI GATC 8 cut(s) 133, 294, 329, 355, 650, 976, 1104, 1231
SchI GAGTC 3 cut(s) 281, 474, 551
ScrFI CCNGG 2 cut(s) 84, 1102
SduI GDGCHC 1 cut(s) 1017
SfaNI GCATC 1 cut(s) 1075
SmlI CTYRAG 1 cut(s) 911
SmoI CTYRAG 1 cut(s) 911
Sse9I AATT 5 cut(s) 175, 267, 485, 791, 1118
SsiI CCGC 6 cut(s) 34, 37, 48, 121, 683, 1127
SspMI CTAG 2 cut(s) 1154, 1218
StyD4I CCNGG 2 cut(s) 82, 1100
TaaI ACNGT 7 cut(s) 220, 409, 477, 533, 736, 835, 991
TaiI ACGT 3 cut(s) 8, 229, 935
TaqI TCGA 3 cut(s) 375, 480, 675
TaqII GACCGA 1 cut(s) 1154
TasI AATT 5 cut(s) 175, 267, 485, 791, 1118
TatI WGTACW 1 cut(s) 127
TauI GCSGC 1 cut(s) 39
TfiI GAWTC 2 cut(s) 62, 1021
Tru1I TTAA 5 cut(s) 543, 606, 786, 986, 996
Tru9I TTAA 5 cut(s) 543, 606, 786, 986, 996
TscAI CASTG 1 cut(s) 79
TseFI GTSAC 3 cut(s) 554, 719, 1135
TseI GCWGC 5 cut(s) 434, 761, 926, 1190, 1193
Tsp45I GTSAC 3 cut(s) 554, 719, 1135
TspDTI ATGAA 7 cut(s) 380, 434, 693, 770, 918, 941, 1191
TspGWI ACGGA 2 cut(s) 14, 154
TspRI CASTG 1 cut(s) 79
VneI GTGCAC 1 cut(s) 1013
XapI RAATTY 2 cut(s) 485, 1118
XmnI GAANNNNTTC 2 cut(s) 371, 1118
XspI CTAG 2 cut(s) 1154, 1218
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.