Rh2CG157300

Pentatricopeptide repeat-containing protein At4g36680

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0014081
Physical Location & Seq
Reverse (-)
13660152 .. 13661820
1669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG157300.1

Sequence Viewer

Length: 1299 bp
ATGAAGATTATGGAGTTGGAAATTGGGATGAACAGAAAAATCGAAACGAATTGGGAGATTTATGGTGTCGGAAAAACCAGATACCATAACGGCATGGACCTCGCCGTCCGCCGCCTCGCCAAGTCCCACCGCTTCGCCGACATCGAGTCCTTCATCGAGTCCCACAAAAACGACCCCAAAATCAAACAAGAGCCGTACCTGTCCACCCTCATCCGATCCTACGGCCGAGCAGGCATGTTCGAGCACGCCTTGAAAACCTATGACCAAATGGACCAGTTGGGTACTCCGAGATCGGCCATTTCTTTCAATTCTTTGCTTACTGCTTGCAATCTTTCAAAACAGTTTGATAAAGTCCCTCAATTTTTCGCTGAAATTCCGAGCAAGTATGGTGTGTCTCCTGATAAAGTGTCGTACGGGATTCTGATCAAGTCGTTTTGCGAAGCTGGGAAGCCGGAGAAGGCCATTGAGACGGTGCGGATGATGGAGGAGAAGGGCATTGAGGTAACTGCGGTGACATTTACAACTATATTCAATGCTTTGTATAAGAAAGGAAATGGTGAAGAGGCTGACAAGTTGTGGAATGAGATGGTGGAGAAGGGTTGTGAGGTTGATGTTGCGGCGTATAATGTGAAGATTATGTATGCTCATGTCAATGGGAGCCCCGAAGATGTGAAGGCTTTGATTGATGAAATCATTGGCGCCGGGTTGAAGCCGGATGCTATTAGCTACAATTACTTGATGACTTGTTATTGCAGGTGTGGGATGATGGAGGAAGCCGAGAAGGTTTACAGGGGGTTGGAGGTGAATAAGCTTAATCCTAATGCGGCCACTTTCAGGACTTTGGTGTTTTATCTCTGTAAGAATGAGTATTTTGAGAAGGCGTACAGGGTTTTCAAGAAGAGTGTGGAGATGCATAAGATACCGGATTTTAATACTTTGAAGTTTTTGGCGGAGGGATTGATGAAGAAAAATATGAGGAAGGAGGCCAAGGGGTTGATTCGGACAGTTAAGAAGAAGTTTCCTCCTAATGTTGTGAATGCCTGGAAGAGGGTGGAGGAGGGTCTTGGTTTGGTCCCTGAAGAACTCGATGCTTCTTGTGTCCCTCATGATGATGGTGAAGAGGTTACACCATCAACAACAGGGATTGGTGGAGAGAACCAAGGATCTGTAAATAGAAAGCAGTGGAAGAAGCCCAAGGCTAAGAAGTGGTTTCCTCCTAATGCTGGGAATGACCGGAAGAAAGTGAGTACTGTTACTTCTTCGGTCCCTGGTGATAGTGCTAAAGAGGCTACAGGATAG

Protein Analysis

432

Amino Acids

48.74

Weight (kDa)

8.88

Isoelectric Point (pI)

32.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 69 - 109 6.1e-06 PPR repeat family
PPR_long PF17177 81 - 190 1.7e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 98 - 146 2.4e-06 PPR repeat family
TPR_24 PF23276 129 - 216 3.2e-10 Fungal tetratrico peptide repeats
PPR_1 PF12854 130 - 162 1.8e-08 PPR repeat
PPR_2 PF13041 133 - 182 8.9e-12 PPR repeat family
PPR PF01535 136 - 166 1.6e-06 PPR repeat
PPR_2 PF13041 170 - 210 2.2e-11 PPR repeat family
PPR PF01535 171 - 201 2.5e-07 PPR repeat
PPR_1 PF12854 235 - 264 5.8e-08 PPR repeat
PPR_2 PF13041 238 - 287 9.9e-11 PPR repeat family
PPR PF01535 241 - 265 4.4e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014081)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18520 AT4G36680
fragaria_vesca FvH4_1g13650
malus_domestica MD02G1151200.v1.1 MD15G1265700.v1.1
prunus_persica Prupe.7G151800_v2.0.a1
pyrus_communis pycom02g11870 pycom15g23140
rosa_chinensis RchiOBHm_Chr2g0102001
rosa_laevigata RLG00000017125
rosa_multiflora Rmu_co8487067.1_g000001
rosa_roxburghii Rroxscaffold_2G00140840
rosa_samantha Rh2AG151900 Rh2BG157300 Rh2CG157300 Rh2DG157400
rosa_wichuraiana Rw2G011860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 744
AasI GACNNNNNNGTC 1 cut(s) 104
Acc36I ACCTGC 1 cut(s) 744
AccB1I GGYRCC 1 cut(s) 698
AciI CCGC 8 cut(s) 109, 112, 130, 475, 509, 617, 824, 950
AclWI GGATC 2 cut(s) 210, 1171
AcoI YGGCCR 3 cut(s) 223, 294, 825
AcsI RAATTY 1 cut(s) 372
AcuI CTGAAG 1 cut(s) 1098
AcyI GRCGYC 1 cut(s) 699
AfaI GTAC 5 cut(s) 197, 283, 413, 884, 1249
AfiI CCNNNNNNNGG 3 cut(s) 834, 1047, 1223
AgsI TTSAA 7 cut(s) 253, 307, 336, 532, 709, 895, 940
AhdI GACNNNNNGTC 1 cut(s) 145
AjnI CCWGG 2 cut(s) 1040, 1267
AluBI AGCT 3 cut(s) 443, 726, 811
AluI AGCT 3 cut(s) 443, 726, 811
Alw21I GWGCWC 1 cut(s) 246
Alw26I GTCTC 2 cut(s) 399, 461
AlwI GGATC 2 cut(s) 210, 1171
AoxI GGCC 5 cut(s) 223, 294, 459, 825, 984
ApoI RAATTY 1 cut(s) 372
AspLEI GCGC 1 cut(s) 701
AspS9I GGNCC 4 cut(s) 97, 271, 1072, 1264
AsuC2I CCSGG 1 cut(s) 703
AsuHPI GGTGA 5 cut(s) 523, 569, 814, 1127, 1283
AvaII GGWCC 4 cut(s) 97, 271, 1072, 1264
BanI GGYRCC 1 cut(s) 698
BanII GRGCYC 1 cut(s) 662
BarI GAAGNNNNNNTAC 2 cut(s) 1240, 1272
Bbv12I GWGCWC 1 cut(s) 246
BccI CCATC 5 cut(s) 475, 580, 760, 1106, 1138
BceAI ACGGC 4 cut(s) 89, 106, 178, 238
BcgI CGANNNNNNTGC 2 cut(s) 82, 116
BciT130I CCWGG 2 cut(s) 1042, 1269
BclI TGATCA 1 cut(s) 423
BcnI CCSGG 1 cut(s) 703
BcoDI GTCTC 2 cut(s) 399, 461
BfmI CTRYAG 1 cut(s) 1290
BfoI RGCGCY 1 cut(s) 702
BfuAI ACCTGC 1 cut(s) 744
BglI GCCNNNNNGGC 1 cut(s) 231
BisI GCNGC 3 cut(s) 112, 618, 825
BlsI GCNGC 3 cut(s) 113, 619, 826
BmcAI AGTACT 1 cut(s) 1249
Bme1390I CCNGG 3 cut(s) 703, 1042, 1269
Bme18I GGWCC 4 cut(s) 97, 271, 1072, 1264
BmeRI GACNNNNNGTC 1 cut(s) 145
BmgT120I GGNCC 4 cut(s) 97, 271, 1072, 1264
BmiI GGNNCC 4 cut(s) 659, 700, 1074, 1266
BmrFI CCNGG 3 cut(s) 703, 1042, 1269
BmsI GCATC 3 cut(s) 706, 900, 1078
BpuMI CCSGG 1 cut(s) 703
BsaBI GATNNNNATC 1 cut(s) 422
BsaHI GRCGYC 1 cut(s) 699
BsaJI CCNNGG 4 cut(s) 987, 1159, 1194, 1267
BsaWI WCCGGW 2 cut(s) 922, 1233
Bsc4I CCNNNNNNNGG 3 cut(s) 834, 1047, 1223
Bse1I ACTGG 1 cut(s) 274
Bse8I GATNNNNATC 1 cut(s) 422
BseBI CCWGG 2 cut(s) 1042, 1269
BseDI CCNNGG 4 cut(s) 987, 1159, 1194, 1267
BseGI GGATG 5 cut(s) 33, 210, 483, 721, 768
BseJI GATNNNNATC 1 cut(s) 422
BseLI CCNNNNNNNGG 3 cut(s) 834, 1047, 1223
BseNI ACTGG 1 cut(s) 274
BseRI GAGGAG 2 cut(s) 500, 1070
BseX3I CGGCCG 1 cut(s) 223
BseYI CCCAGC 2 cut(s) 443, 1223
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 5 cut(s) 225, 296, 461, 827, 986
BshNI GGYRCC 1 cut(s) 698
BsiEI CGRYCG 1 cut(s) 226
BsiHKAI GWGCWC 1 cut(s) 246
BsiSI CCGG 5 cut(s) 452, 702, 713, 923, 1234
BsiWI CGTACG 1 cut(s) 411
BslFI GGGAC 6 cut(s) 109, 145, 338, 1058, 1085, 1250
BslI CCNNNNNNNGG 3 cut(s) 834, 1047, 1223
BsmAI GTCTC 2 cut(s) 399, 461
BsmBI CGTCTC 1 cut(s) 461
BsmFI GGGAC 6 cut(s) 109, 145, 338, 1058, 1085, 1250
BsmI GAATGC 1 cut(s) 1042
BsnI GGCC 5 cut(s) 225, 296, 461, 827, 986
Bsp1286I GDGCHC 2 cut(s) 246, 662
Bsp143I GATC 4 cut(s) 215, 290, 423, 1163
BspACI CCGC 8 cut(s) 109, 112, 130, 475, 509, 617, 824, 950
BspANI GGCC 5 cut(s) 225, 296, 461, 827, 986
BspHI TCATGA 1 cut(s) 1105
BspLI GGNNCC 4 cut(s) 659, 700, 1074, 1266
BspMI ACCTGC 1 cut(s) 744
BspPI GGATC 2 cut(s) 210, 1171
BspT107I GGYRCC 1 cut(s) 698
BsrI ACTGG 1 cut(s) 274
BssECI CCNNGG 4 cut(s) 987, 1159, 1194, 1267
BssMI GATC 4 cut(s) 215, 290, 423, 1163
BssNI GRCGYC 1 cut(s) 699
BssT1I CCWWGG 3 cut(s) 987, 1159, 1194
Bst2UI CCWGG 2 cut(s) 1042, 1269
Bst4CI ACNGT 4 cut(s) 342, 472, 1006, 1252
Bst6I CTCTTC 4 cut(s) 555, 893, 1040, 1113
BstACI GRCGYC 1 cut(s) 699
BstC8I GCNNGC 3 cut(s) 232, 246, 325
BstDEI CTNAG 1 cut(s) 1200
BstENI CCTNNNNNAGG 1 cut(s) 1045
BstF5I GGATG 5 cut(s) 33, 210, 483, 721, 768
BstH2I RGCGCY 1 cut(s) 702
BstHHI GCGC 1 cut(s) 701
BstKTI GATC 4 cut(s) 218, 293, 426, 1166
BstMAI GTCTC 2 cut(s) 399, 461
BstMBI GATC 4 cut(s) 215, 290, 423, 1163
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 2 cut(s) 231, 1286
BstNI CCWGG 2 cut(s) 1042, 1269
BstNSI RCATGY 1 cut(s) 238
BstSCI CCNGG 3 cut(s) 701, 1040, 1267
BstSFI CTRYAG 1 cut(s) 1290
BstX2I RGATCY 1 cut(s) 1163
BstYI RGATCY 1 cut(s) 1163
BstZI CGGCCG 1 cut(s) 223
BsuRI GGCC 5 cut(s) 225, 296, 461, 827, 986
BtsCI GGATG 5 cut(s) 33, 210, 483, 721, 768
BtsI GCAGTG 1 cut(s) 1187
BtsIMutI CAGTG 1 cut(s) 1187
BveI ACCTGC 1 cut(s) 744
Cac8I GCNNGC 3 cut(s) 232, 246, 325
CciI TCATGA 1 cut(s) 1105
CfoI GCGC 1 cut(s) 701
Cfr13I GGNCC 4 cut(s) 97, 271, 1072, 1264
Csp6I GTAC 5 cut(s) 196, 282, 412, 883, 1248
CviAII CATG 4 cut(s) 94, 235, 647, 1106
CviQI GTAC 5 cut(s) 196, 282, 412, 883, 1248
DdeI CTNAG 1 cut(s) 1200
DinI GGCGCC 1 cut(s) 700
DpnI GATC 4 cut(s) 217, 292, 425, 1165
DpnII GATC 4 cut(s) 215, 290, 423, 1163
DrdI GACNNNNNNGTC 1 cut(s) 104
DriI GACNNNNNGTC 1 cut(s) 145
DseDI GACNNNNNNGTC 1 cut(s) 104
EaeI YGGCCR 3 cut(s) 223, 294, 825
EagI CGGCCG 1 cut(s) 223
Eam1104I CTCTTC 4 cut(s) 555, 893, 1040, 1113
Eam1105I GACNNNNNGTC 1 cut(s) 145
EarI CTCTTC 4 cut(s) 555, 893, 1040, 1113
EciI GGCGGA 2 cut(s) 98, 965
EclXI CGGCCG 1 cut(s) 223
Eco130I CCWWGG 3 cut(s) 987, 1159, 1194
Eco24I GRGCYC 1 cut(s) 662
Eco47I GGWCC 4 cut(s) 97, 271, 1072, 1264
Eco52I CGGCCG 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 1098
EcoNI CCTNNNNNAGG 1 cut(s) 1045
EcoRII CCWGG 2 cut(s) 1040, 1267
EcoT14I CCWWGG 3 cut(s) 987, 1159, 1194
EcoT22I ATGCAT 1 cut(s) 915
EcoT38I GRGCYC 1 cut(s) 662
EgeI GGCGCC 1 cut(s) 700
EheI GGCGCC 1 cut(s) 700
ErhI CCWWGG 3 cut(s) 987, 1159, 1194
Esp3I CGTCTC 1 cut(s) 461
FaeI CATG 4 cut(s) 97, 238, 650, 1109
FaqI GGGAC 6 cut(s) 109, 145, 338, 1058, 1085, 1250
FatI CATG 4 cut(s) 93, 234, 646, 1105
FbaI TGATCA 1 cut(s) 423
Fnu4HI GCNGC 3 cut(s) 112, 618, 825
FokI GGATG 5 cut(s) 40, 197, 490, 728, 775
FriOI GRGCYC 1 cut(s) 662
Fsp4HI GCNGC 3 cut(s) 112, 618, 825
GlaI GCGC 1 cut(s) 700
GluI GCNGC 3 cut(s) 112, 618, 825
GsaI CCCAGC 2 cut(s) 447, 1227
HaeII RGCGCY 1 cut(s) 702
HaeIII GGCC 5 cut(s) 225, 296, 461, 827, 986
HapII CCGG 5 cut(s) 452, 702, 713, 923, 1234
HhaI GCGC 1 cut(s) 701
Hin1I GRCGYC 1 cut(s) 699
Hin1II CATG 4 cut(s) 97, 238, 650, 1109
Hin6I GCGC 1 cut(s) 699
HinP1I GCGC 1 cut(s) 699
HindIII AAGCTT 1 cut(s) 809
HinfI GANTC 4 cut(s) 146, 158, 418, 997
HpaII CCGG 5 cut(s) 452, 702, 713, 923, 1234
HphI GGTGA 5 cut(s) 523, 569, 814, 1127, 1283
Hpy166II GTNNAC 2 cut(s) 204, 787
Hpy188I TCNGA 6 cut(s) 71, 215, 288, 378, 423, 1002
Hpy188III TCNNGA 4 cut(s) 398, 835, 895, 1106
Hpy8I GTNNAC 2 cut(s) 204, 787
HpyAV CCTTC 8 cut(s) 160, 451, 484, 589, 667, 775, 871, 973
HpyCH4III ACNGT 4 cut(s) 342, 472, 1006, 1252
HpyCH4V TGCA 3 cut(s) 327, 753, 913
HpyF10VI GCNNNNNNNGC 2 cut(s) 231, 1286
HpyF3I CTNAG 1 cut(s) 1200
Hsp92I GRCGYC 1 cut(s) 699
Hsp92II CATG 4 cut(s) 97, 238, 650, 1109
HspAI GCGC 1 cut(s) 699
KasI GGCGCC 1 cut(s) 698
Ksp22I TGATCA 1 cut(s) 423
Kzo9I GATC 4 cut(s) 215, 290, 423, 1163
LmnI GCTCC 1 cut(s) 657
LweI GCATC 3 cut(s) 706, 900, 1078
MaeIII GTNAC 4 cut(s) 502, 511, 1123, 1252
MalI GATC 4 cut(s) 217, 292, 425, 1165
MboI GATC 4 cut(s) 215, 290, 423, 1163
MflI RGATCY 1 cut(s) 1163
MhlI GDGCHC 2 cut(s) 246, 662
MluCI AATT 6 cut(s) 21, 49, 307, 359, 372, 730
Mly113I GGCGCC 1 cut(s) 699
MlyI GAGTC 2 cut(s) 155, 167
MmeI TCCRAC 2 cut(s) 49, 777
Mph1103I ATGCAT 1 cut(s) 915
MseI TTAA 3 cut(s) 813, 930, 1008
MslI CAYNNNNRTG 2 cut(s) 651, 1110
MspI CCGG 5 cut(s) 452, 702, 713, 923, 1234
MspR9I CCNGG 3 cut(s) 703, 1042, 1269
Mva1269I GAATGC 1 cut(s) 1042
MvaI CCWGG 2 cut(s) 1042, 1269
MwoI GCNNNNNNNGC 2 cut(s) 231, 1286
NarI GGCGCC 1 cut(s) 699
NciI CCSGG 1 cut(s) 703
NdeII GATC 4 cut(s) 215, 290, 423, 1163
NlaIII CATG 4 cut(s) 97, 238, 650, 1109
NlaIV GGNNCC 4 cut(s) 659, 700, 1074, 1266
NmeAIII GCCGAG 2 cut(s) 251, 802
NmuCI GTSAC 1 cut(s) 511
NsiI ATGCAT 1 cut(s) 915
NspI RCATGY 1 cut(s) 238
PagI TCATGA 1 cut(s) 1105
PaqCI CACCTGC 1 cut(s) 744
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PctI GAATGC 1 cut(s) 1042
PfeI GAWTC 2 cut(s) 418, 997
Pfl23II CGTACG 1 cut(s) 411
PkrI GCNGC 3 cut(s) 113, 619, 826
PleI GAGTC 2 cut(s) 154, 166
PluTI GGCGCC 1 cut(s) 702
PpsI GAGTC 2 cut(s) 154, 166
Psp6I CCWGG 2 cut(s) 1040, 1267
PspFI CCCAGC 2 cut(s) 443, 1223
PspGI CCWGG 2 cut(s) 1040, 1267
PspLI CGTACG 1 cut(s) 411
PspN4I GGNNCC 4 cut(s) 659, 700, 1074, 1266
PspPI GGNCC 4 cut(s) 97, 271, 1072, 1264
PsuI RGATCY 1 cut(s) 1163
RsaI GTAC 5 cut(s) 197, 283, 413, 884, 1249
RsaNI GTAC 5 cut(s) 196, 282, 412, 883, 1248
RseI CAYNNNNRTG 2 cut(s) 651, 1110
SaqAI TTAA 3 cut(s) 813, 930, 1008
SatI GCNGC 3 cut(s) 112, 618, 825
Sau3AI GATC 4 cut(s) 215, 290, 423, 1163
Sau96I GGNCC 4 cut(s) 97, 271, 1072, 1264
ScaI AGTACT 1 cut(s) 1249
SchI GAGTC 2 cut(s) 155, 167
ScrFI CCNGG 3 cut(s) 703, 1042, 1269
SduI GDGCHC 2 cut(s) 246, 662
SfaNI GCATC 3 cut(s) 706, 900, 1078
SfcI CTRYAG 1 cut(s) 1290
SfoI GGCGCC 1 cut(s) 700
SinI GGWCC 4 cut(s) 97, 271, 1072, 1264
SmiMI CAYNNNNRTG 2 cut(s) 651, 1110
Sse9I AATT 6 cut(s) 21, 49, 307, 359, 372, 730
SsiI CCGC 8 cut(s) 109, 112, 130, 475, 509, 617, 824, 950
SspDI GGCGCC 1 cut(s) 698
StyD4I CCNGG 3 cut(s) 701, 1040, 1267
StyI CCWWGG 3 cut(s) 987, 1159, 1194
TaaI ACNGT 4 cut(s) 342, 472, 1006, 1252
TaqI TCGA 5 cut(s) 42, 144, 156, 240, 1086
TaqII GACCGA 1 cut(s) 1252
TasI AATT 6 cut(s) 21, 49, 307, 359, 372, 730
TatI WGTACW 1 cut(s) 1247
TauI GCSGC 3 cut(s) 114, 620, 827
TfiI GAWTC 2 cut(s) 418, 997
Tru1I TTAA 3 cut(s) 813, 930, 1008
Tru9I TTAA 3 cut(s) 813, 930, 1008
TscAI CASTG 1 cut(s) 1187
TseFI GTSAC 1 cut(s) 511
Tsp45I GTSAC 1 cut(s) 511
TspDTI ATGAA 5 cut(s) 17, 44, 142, 702, 977
TspRI CASTG 1 cut(s) 1187
VpaK11BI GGWCC 4 cut(s) 97, 271, 1072, 1264
XagI CCTNNNNNAGG 1 cut(s) 1045
XapI RAATTY 1 cut(s) 372
XceI RCATGY 1 cut(s) 238
ZrmI AGTACT 1 cut(s) 1249
Zsp2I ATGCAT 1 cut(s) 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.