Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0014081

Physical Location & Seq

Genomic Coordinates Reverse (-)

12477164 .. 12478381

Gene Structure 1218 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1151200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1218 bp

ATGTCATCTTCGATTCCCCTCCGCCACCTCCGCCATCTATCCACCACCACCGCCAAGTCTACCCTTTCCATCTCAAAAGCGAAATCAAAGCTCCGAACCGAGTACGACCCTGACAAAGCCCTCCAGATTTATTCCTCCGTCTCCGAACACTACTCTTCTCCCACCTCGTCTCGCTACACCCAGGATCTCACCGTCCGCCGCCTCGCCAAGTCCCACCGCTTTGCTGACATCGAATCCTTCATTGAATCCCACAAAAATGACCCCAAAATCACCCAGGAGCCGTTCCTGTCCACCCTCATCCGATCCTACGGCCGCGCCGGCATGTTTGACCAGGCATTGAGGACCTTTGATCAGATGGACCAGCTGGGTACTCCTAGAACCGCCATTTCCTTCAACACTCTGTTGTCTGCCTGCAACGATTCTAAGCATTTCGACAGAGTCCCCCAGCTTTTCGACGAAATTCCCAACAAGCATGGGGTCTCCCCTGATAAAGTGTCGTATGGTATACTGATTAAGTCGTATTGTGCTGCGGATAAGCCCGAGAAGGCGATGGAGACGCTGAGGTTGATGGAGGAGAAGGGTATTGAGATCACTGCGGTGACGTTCACTACAATTTTCAATGCTTTGTACAAGAAGGGCAATGGCGAAGAGGCAGAGAAGTTGTGGGATGAGATGGTGAAGAAGGGCGTTGAGGTTGATGCTGCGGCTTATAATGTGAAGATTATGTATGTCCATGGCGGGAGCGCGGACAATGTGAAAGCTTTGATAGAGGAAATGGCGAATGCCGGGCTGAAACCGGATACTATTAGTTACAATTACTTGATGACTTGCTATTGCAGGAATGGGATGATGGAAGAAGCTGTCAAGGTTTATGAGGGGTTGGAGGGAAATGCTTGTAATCCAAATGCTGCAACTTTTCGAACTTTGATATTTTATTTGTGTGAAAGTGGGGATTATGACAAGGCGTATAAAGTTTACAAGAGAAGTGTGGAGGTGCATAAGATCCCCGATTTCAATACAATGAAGTATTTGGCTGAAGGATTGGTGAAAAAGAAGAAGATGAAGGAGGCCAAGGGATTGATACGCACGATCAAGAAGAAGTTCCCTCCTAATGTTTTGGTTGCCTGGAAGAAATTGGAGCAGGGTCTAGGTTTGGCTTCTTCTGATACTGGTGCGTCTTCAGTCGCAGATGAGGAGGCTAAAGAGGCTACAGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016020 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0080156 GO:0090304 GO:0090305 GO:0097159 GO:1900864 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

406

Amino Acids

45.37

Weight (kDa)

8.72

Isoelectric Point (pI)

40.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	98 - 138	1.5e-06	PPR repeat family
PPR_long	PF17177	110 - 228	8.7e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	128 - 175	6.6e-08	PPR repeat family
TPR_24	PF23276	157 - 241	1.5e-10	Fungal tetratrico peptide repeats
PPR_1	PF12854	159 - 191	7.9e-07	PPR repeat
PPR_2	PF13041	162 - 211	1.1e-11	PPR repeat family
PPR	PF01535	165 - 195	1.6e-06	PPR repeat
PPR_2	PF13041	200 - 239	9.4e-11	PPR repeat family
PPR	PF01535	200 - 230	1.6e-07	PPR repeat
PPR_long	PF17177	220 - 340	1.8e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	255 - 310	2.4e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	263 - 292	1.3e-08	PPR repeat
PPR_2	PF13041	266 - 310	4.5e-12	PPR repeat family
PPR	PF01535	269 - 294	1.6e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014081)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G18520 AT4G36680
fragaria_vesca	FvH4_1g13650
malus_domestica	MD02G1151200.v1.1 MD15G1265700.v1.1
prunus_persica	Prupe.7G151800_v2.0.a1
pyrus_communis	pycom02g11870 pycom15g23140
rosa_chinensis	RchiOBHm_Chr2g0102001
rosa_laevigata	RLG00000017125
rosa_multiflora	Rmu_co8487067.1_g000001
rosa_roxburghii	Rroxscaffold_2G00140840
rosa_samantha	Rh2AG151900 Rh2BG157300 Rh2CG157300 Rh2DG157400
rosa_wichuraiana	Rw2G011860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	711
AccI	GTMKAC	2 cut(s)	59, 505
AccII	CGCG	2 cut(s)	315, 746
AclWI	GGATC	3 cut(s)	192, 297, 997
AcoI	YGGCCR	1 cut(s)	310
AcsI	RAATTY	1 cut(s)	459
AcuI	CTGAAG	2 cut(s)	1056, 1164
AfaI	GTAC	3 cut(s)	104, 370, 629
AgsI	TTSAA	4 cut(s)	245, 394, 619, 1015
AjnI	CCWGG	4 cut(s)	180, 273, 330, 1124
AleI	CACNNNNGTG	1 cut(s)	596
AluBI	AGCT	5 cut(s)	91, 364, 448, 761, 860
AluI	AGCT	5 cut(s)	91, 364, 448, 761, 860
Alw26I	GTCTC	4 cut(s)	145, 174, 484, 548
AlwI	GGATC	3 cut(s)	192, 297, 997
Ama87I	CYCGRG	1 cut(s)	539
AoxI	GGCC	2 cut(s)	310, 1068
ApeKI	GCWGC	3 cut(s)	527, 701, 908
ApoI	RAATTY	1 cut(s)	459
Asp700I	GAANNNNTTC	1 cut(s)	1100
AspLEI	GCGC	2 cut(s)	317, 746
AspS9I	GGNCC	2 cut(s)	342, 358
AsuC2I	CCSGG	1 cut(s)	787
AsuHPI	GGTGA	5 cut(s)	181, 262, 610, 688, 1057
AsuII	TTCGAA	1 cut(s)	919
AvaI	CYCGRG	1 cut(s)	539
AvaII	GGWCC	2 cut(s)	342, 358
BbsI	GAAGAC	1 cut(s)	1170
BbvCI	CCTCAGC	1 cut(s)	560
BbvI	GCAGC	3 cut(s)	514, 688, 895
BccI	CCATC	7 cut(s)	42, 77, 349, 544, 562, 667, 844
BceAI	ACGGC	2 cut(s)	265, 325
BciT130I	CCWGG	4 cut(s)	182, 275, 332, 1126
BciVI	GTATCC	1 cut(s)	793
BclI	TGATCA	1 cut(s)	349
BcnI	CCSGG	1 cut(s)	787
BcoDI	GTCTC	4 cut(s)	145, 174, 484, 548
BfaI	CTAG	2 cut(s)	375, 1148
BfmI	CTRYAG	1 cut(s)	1209
BfuI	GTATCC	1 cut(s)	793
BglI	GCCNNNNNGGC	1 cut(s)	318
BisI	GCNGC	6 cut(s)	199, 313, 528, 702, 705, 909
BlsI	GCNGC	6 cut(s)	200, 314, 529, 703, 706, 910
Bme1390I	CCNGG	5 cut(s)	182, 275, 332, 787, 1126
Bme18I	GGWCC	2 cut(s)	342, 358
BmeT110I	CYCGRG	1 cut(s)	539
BmgT120I	GGNCC	2 cut(s)	342, 358
BmiI	GGNNCC	1 cut(s)	279
BmrFI	CCNGG	5 cut(s)	182, 275, 332, 787, 1126
BmsI	GCATC	1 cut(s)	688
BpiI	GAAGAC	1 cut(s)	1170
BpmI	CTGGAG	1 cut(s)	107
Bpu10I	CCTNAGC	1 cut(s)	560
Bpu14I	TTCGAA	1 cut(s)	919
BpuMI	CCSGG	1 cut(s)	787
BsaI	GGTCTC	1 cut(s)	484
BsaJI	CCNNGG	4 cut(s)	180, 273, 733, 1071
BsaWI	WCCGGW	1 cut(s)	796
Bse118I	RCCGGY	1 cut(s)	317
Bse1I	ACTGG	1 cut(s)	1174
Bse3DI	GCAATG	1 cut(s)	646
BseBI	CCWGG	4 cut(s)	182, 275, 332, 1126
BseDI	CCNNGG	4 cut(s)	180, 273, 733, 1071
BseGI	GGATG	3 cut(s)	297, 673, 852
BseMI	GCAATG	1 cut(s)	646
BseMII	CTCAG	1 cut(s)	551
BseNI	ACTGG	1 cut(s)	1174
BseRI	GAGGAG	2 cut(s)	587, 1208
BseX3I	CGGCCG	1 cut(s)	310
BseXI	GCAGC	3 cut(s)	514, 688, 895
BseYI	CCCAGC	2 cut(s)	364, 444
Bsh1236I	CGCG	2 cut(s)	315, 746
Bsh1285I	CGRYCG	1 cut(s)	313
BshFI	GGCC	2 cut(s)	312, 1070
BsiEI	CGRYCG	1 cut(s)	313
BsiHKCI	CYCGRG	1 cut(s)	539
BsiSI	CCGG	3 cut(s)	318, 786, 797
BslFI	GGGAC	2 cut(s)	196, 425
BsmAI	GTCTC	4 cut(s)	145, 174, 484, 548
BsmBI	CGTCTC	3 cut(s)	145, 174, 548
BsmFI	GGGAC	2 cut(s)	196, 425
BsmI	GAATGC	1 cut(s)	787
BsnI	GGCC	2 cut(s)	312, 1070
Bso31I	GGTCTC	1 cut(s)	484
BsoBI	CYCGRG	1 cut(s)	539
Bsp119I	TTCGAA	1 cut(s)	919
Bsp1407I	TGTACA	1 cut(s)	627
Bsp143I	GATC	6 cut(s)	184, 302, 349, 588, 1002, 1089
Bsp19I	CCATGG	1 cut(s)	733
BspANI	GGCC	2 cut(s)	312, 1070
BspCNI	CTCAG	1 cut(s)	552
BspFNI	CGCG	2 cut(s)	315, 746
BspLI	GGNNCC	1 cut(s)	279
BspPI	GGATC	3 cut(s)	192, 297, 997
BspT104I	TTCGAA	1 cut(s)	919
BspTNI	GGTCTC	1 cut(s)	484
BsrDI	GCAATG	1 cut(s)	646
BsrFI	RCCGGY	1 cut(s)	317
BsrGI	TGTACA	1 cut(s)	627
BsrI	ACTGG	1 cut(s)	1174
BssAI	RCCGGY	1 cut(s)	317
BssECI	CCNNGG	4 cut(s)	180, 273, 733, 1071
BssMI	GATC	6 cut(s)	184, 302, 349, 588, 1002, 1089
BssNAI	GTATAC	1 cut(s)	506
BssT1I	CCWWGG	2 cut(s)	733, 1071
Bst1107I	GTATAC	1 cut(s)	506
Bst2UI	CCWGG	4 cut(s)	182, 275, 332, 1126
Bst4CI	ACNGT	1 cut(s)	193
Bst6I	CTCTTC	2 cut(s)	160, 642
BstAUI	TGTACA	1 cut(s)	627
BstBI	TTCGAA	1 cut(s)	919
BstC8I	GCNNGC	2 cut(s)	319, 412
BstDEI	CTNAG	2 cut(s)	423, 560
BstDSI	CCRYGG	1 cut(s)	733
BstF5I	GGATG	3 cut(s)	297, 673, 852
BstFNI	CGCG	2 cut(s)	315, 746
BstHHI	GCGC	2 cut(s)	317, 746
BstKTI	GATC	6 cut(s)	187, 305, 352, 591, 1005, 1092
BstMAI	GTCTC	4 cut(s)	145, 174, 484, 548
BstMBI	GATC	6 cut(s)	184, 302, 349, 588, 1002, 1089
BstMCI	CGRYCG	1 cut(s)	313
BstMWI	GCNNNNNNNGC	3 cut(s)	30, 318, 1205
BstNI	CCWGG	4 cut(s)	182, 275, 332, 1126
BstNSI	RCATGY	1 cut(s)	325
BstSCI	CCNGG	5 cut(s)	180, 273, 330, 785, 1124
BstSFI	CTRYAG	1 cut(s)	1209
BstUI	CGCG	2 cut(s)	315, 746
BstV1I	GCAGC	3 cut(s)	514, 688, 895
BstV2I	GAAGAC	1 cut(s)	1170
BstX2I	RGATCY	2 cut(s)	184, 1002
BstYI	RGATCY	2 cut(s)	184, 1002
BstZ17I	GTATAC	1 cut(s)	506
BstZI	CGGCCG	1 cut(s)	310
BsuI	GTATCC	1 cut(s)	793
BsuRI	GGCC	2 cut(s)	312, 1070
BtgI	CCRYGG	1 cut(s)	733
BtgZI	GCGATG	1 cut(s)	563
BtsCI	GGATG	3 cut(s)	297, 673, 852
BtsI	GCAGTG	1 cut(s)	591
BtsIMutI	CAGTG	1 cut(s)	591
Cac8I	GCNNGC	2 cut(s)	319, 412
CfoI	GCGC	2 cut(s)	317, 746
Cfr10I	RCCGGY	1 cut(s)	317
Cfr13I	GGNCC	2 cut(s)	342, 358
CseI	GACGC	2 cut(s)	565, 1164
Csp6I	GTAC	3 cut(s)	103, 369, 628
CviAII	CATG	3 cut(s)	322, 473, 734
CviQI	GTAC	3 cut(s)	103, 369, 628
DdeI	CTNAG	2 cut(s)	423, 560
DpnI	GATC	6 cut(s)	186, 304, 351, 590, 1004, 1091
DpnII	GATC	6 cut(s)	184, 302, 349, 588, 1002, 1089
EaeI	YGGCCR	1 cut(s)	310
EagI	CGGCCG	1 cut(s)	310
Eam1104I	CTCTTC	2 cut(s)	160, 642
EarI	CTCTTC	2 cut(s)	160, 642
EciI	GGCGGA	3 cut(s)	11, 20, 185
EclXI	CGGCCG	1 cut(s)	310
Eco130I	CCWWGG	2 cut(s)	733, 1071
Eco31I	GGTCTC	1 cut(s)	484
Eco47I	GGWCC	2 cut(s)	342, 358
Eco52I	CGGCCG	1 cut(s)	310
Eco57I	CTGAAG	2 cut(s)	1056, 1164
Eco88I	CYCGRG	1 cut(s)	539
EcoO109I	RGGNCCY	1 cut(s)	342
EcoRII	CCWGG	4 cut(s)	180, 273, 330, 1124
EcoT14I	CCWWGG	2 cut(s)	733, 1071
ErhI	CCWWGG	2 cut(s)	733, 1071
Esp3I	CGTCTC	3 cut(s)	145, 174, 548
FaeI	CATG	3 cut(s)	325, 476, 737
FaqI	GGGAC	2 cut(s)	196, 425
FatI	CATG	3 cut(s)	321, 472, 733
FauI	CCCGC	1 cut(s)	731
FbaI	TGATCA	1 cut(s)	349
FblI	GTMKAC	2 cut(s)	59, 505
Fnu4HI	GCNGC	6 cut(s)	199, 313, 528, 702, 705, 909
FokI	GGATG	3 cut(s)	284, 680, 859
Fsp4HI	GCNGC	6 cut(s)	199, 313, 528, 702, 705, 909
FspBI	CTAG	2 cut(s)	375, 1148
GlaI	GCGC	2 cut(s)	316, 745
GluI	GCNGC	6 cut(s)	199, 313, 528, 702, 705, 909
GsaI	CCCAGC	2 cut(s)	368, 448
GsuI	CTGGAG	1 cut(s)	107
HaeIII	GGCC	2 cut(s)	312, 1070
HapII	CCGG	3 cut(s)	318, 786, 797
HgaI	GACGC	2 cut(s)	565, 1164
HhaI	GCGC	2 cut(s)	317, 746
Hin1II	CATG	3 cut(s)	325, 476, 737
Hin6I	GCGC	2 cut(s)	315, 744
HinP1I	GCGC	2 cut(s)	315, 744
HindIII	AAGCTT	1 cut(s)	759
HinfI	GANTC	5 cut(s)	13, 233, 245, 419, 438
HpaII	CCGG	3 cut(s)	318, 786, 797
HphI	GGTGA	5 cut(s)	181, 262, 610, 688, 1057
Hpy166II	GTNNAC	5 cut(s)	60, 291, 506, 606, 976
Hpy188I	TCNGA	5 cut(s)	95, 145, 302, 354, 1165
Hpy188III	TCNNGA	2 cut(s)	124, 1093
Hpy8I	GTNNAC	5 cut(s)	60, 291, 506, 606, 976
Hpy99I	CGWCG	1 cut(s)	458
HpyAV	CCTTC	8 cut(s)	247, 400, 538, 571, 628, 676, 1031, 1057
HpyCH4III	ACNGT	1 cut(s)	193
HpyCH4IV	ACGT	1 cut(s)	602
HpyCH4V	TGCA	4 cut(s)	414, 837, 911, 997
HpyF10VI	GCNNNNNNNGC	3 cut(s)	30, 318, 1205
HpyF3I	CTNAG	2 cut(s)	423, 560
HpySE526I	ACGT	1 cut(s)	602
Hsp92II	CATG	3 cut(s)	325, 476, 737
HspAI	GCGC	2 cut(s)	315, 744
KroI	GCCGGC	1 cut(s)	317
KroNI	GCCGGC	1 cut(s)	319
Ksp22I	TGATCA	1 cut(s)	349
Kzo9I	GATC	6 cut(s)	184, 302, 349, 588, 1002, 1089
LmnI	GCTCC	4 cut(s)	96, 277, 741, 1138
Lsp1109I	GCAGC	3 cut(s)	514, 688, 895
LweI	GCATC	1 cut(s)	688
MaeI	CTAG	2 cut(s)	375, 1148
MaeII	ACGT	1 cut(s)	602
MaeIII	GTNAC	2 cut(s)	598, 809
MalI	GATC	6 cut(s)	186, 304, 351, 590, 1004, 1091
MboI	GATC	6 cut(s)	184, 302, 349, 588, 1002, 1089
MflI	RGATCY	2 cut(s)	184, 1002
MluCI	AATT	4 cut(s)	459, 612, 814, 1133
MlyI	GAGTC	1 cut(s)	447
MmeI	TCCRAC	1 cut(s)	861
MroNI	GCCGGC	1 cut(s)	317
MroXI	GAANNNNTTC	1 cut(s)	1100
MseI	TTAA	1 cut(s)	513
MslI	CAYNNNNRTG	2 cut(s)	255, 596
MspA1I	CMGCKG	1 cut(s)	364
MspI	CCGG	3 cut(s)	318, 786, 797
MspR9I	CCNGG	5 cut(s)	182, 275, 332, 787, 1126
Mva1269I	GAATGC	1 cut(s)	787
MvaI	CCWGG	4 cut(s)	182, 275, 332, 1126
MvnI	CGCG	2 cut(s)	315, 746
MwoI	GCNNNNNNNGC	3 cut(s)	30, 318, 1205
NaeI	GCCGGC	1 cut(s)	319
NciI	CCSGG	1 cut(s)	787
NcoI	CCATGG	1 cut(s)	733
NdeII	GATC	6 cut(s)	184, 302, 349, 588, 1002, 1089
NgoMIV	GCCGGC	1 cut(s)	317
NlaIII	CATG	3 cut(s)	325, 476, 737
NlaIV	GGNNCC	1 cut(s)	279
NmuCI	GTSAC	1 cut(s)	598
NspI	RCATGY	1 cut(s)	325
NspV	TTCGAA	1 cut(s)	919
OliI	CACNNNNGTG	1 cut(s)	596
PctI	GAATGC	1 cut(s)	787
PdiI	GCCGGC	1 cut(s)	319
PdmI	GAANNNNTTC	1 cut(s)	1100
PfeI	GAWTC	4 cut(s)	13, 233, 245, 419
PflFI	GACNNNGTC	1 cut(s)	437
PkrI	GCNGC	6 cut(s)	200, 314, 529, 703, 706, 910
PleI	GAGTC	1 cut(s)	446
PpsI	GAGTC	1 cut(s)	446
PpuMI	RGGWCCY	1 cut(s)	342
PsiI	TTATAA	1 cut(s)	711
Psp5II	RGGWCCY	1 cut(s)	342
Psp6I	CCWGG	4 cut(s)	180, 273, 330, 1124
PspFI	CCCAGC	2 cut(s)	364, 444
PspGI	CCWGG	4 cut(s)	180, 273, 330, 1124
PspN4I	GGNNCC	1 cut(s)	279
PspPI	GGNCC	2 cut(s)	342, 358
PspPPI	RGGWCCY	1 cut(s)	342
PsuI	RGATCY	2 cut(s)	184, 1002
PsyI	GACNNNGTC	1 cut(s)	437
PvuII	CAGCTG	1 cut(s)	364
RsaI	GTAC	3 cut(s)	104, 370, 629
RsaNI	GTAC	3 cut(s)	103, 369, 628
RseI	CAYNNNNRTG	2 cut(s)	255, 596
SaqAI	TTAA	1 cut(s)	513
SatI	GCNGC	6 cut(s)	199, 313, 528, 702, 705, 909
Sau3AI	GATC	6 cut(s)	184, 302, 349, 588, 1002, 1089
Sau96I	GGNCC	2 cut(s)	342, 358
SchI	GAGTC	1 cut(s)	447
ScrFI	CCNGG	5 cut(s)	182, 275, 332, 787, 1126
SfaNI	GCATC	1 cut(s)	688
SfcI	CTRYAG	1 cut(s)	1209
SfuI	TTCGAA	1 cut(s)	919
SinI	GGWCC	2 cut(s)	342, 358
SmiMI	CAYNNNNRTG	2 cut(s)	255, 596
Sse9I	AATT	4 cut(s)	459, 612, 814, 1133
SspMI	CTAG	2 cut(s)	375, 1148
StyD4I	CCNGG	5 cut(s)	180, 273, 330, 785, 1124
StyI	CCWWGG	2 cut(s)	733, 1071
TaaI	ACNGT	1 cut(s)	193
TaiI	ACGT	1 cut(s)	605
TaqI	TCGA	5 cut(s)	11, 231, 432, 453, 919
TasI	AATT	4 cut(s)	459, 612, 814, 1133
TatI	WGTACW	1 cut(s)	627
TauI	GCSGC	3 cut(s)	201, 315, 707
TfiI	GAWTC	4 cut(s)	13, 233, 245, 419
Tru1I	TTAA	1 cut(s)	513
Tru9I	TTAA	1 cut(s)	513
TscAI	CASTG	1 cut(s)	598
TseFI	GTSAC	1 cut(s)	598
TseI	GCWGC	3 cut(s)	527, 701, 908
Tsp45I	GTSAC	1 cut(s)	598
TspDTI	ATGAA	3 cut(s)	229, 1037, 1076
TspGWI	ACGGA	1 cut(s)	127
TspRI	CASTG	1 cut(s)	598
Tth111I	GACNNNGTC	1 cut(s)	437
VpaK11BI	GGWCC	2 cut(s)	342, 358
XapI	RAATTY	1 cut(s)	459
XceI	RCATGY	1 cut(s)	325
XmiI	GTMKAC	2 cut(s)	59, 505
XmnI	GAANNNNTTC	1 cut(s)	1100
XspI	CTAG	2 cut(s)	375, 1148

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.