AT2G18876

Afadin- and alpha-actinin-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006767
Physical Location & Seq
Forward (+)
8169546 .. 8172523
2978 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G18876.1

Sequence Viewer

Length: 1149 bp
ATGTCATTGACTGTACCGGAGTTCGATTTCAGATTGACGCCACAGTTTGGTTTCTCACAGTCACCGATCGGGGAGCGTAATTTCGCGGATGTTGATAATCTGGAGAACTGTATTAAGTATTTGAATCAGAGCTTAGTCACCTCTGGATTCTCTGCTTCGCTTGACCTCTTCGCTACCGATCCTGTTTCGATTGCTAGAACTTGCAATTGCGTATACGCTTTGATTCAACAGAGGCAACGAGATGTGGAATTTAGAGAATCTGCTAATGATCAGAGACAACGACTTTTATCCGATATGGCGAGACTCGAAGCAAAAGTTGAAAGGCTTGAGACTCAATTGCAAGCTAAAGAAAGGGAACTTGGTTCGGTGACAAGAACGGAAGCAAAAAATACAGCGGCTTTAAAGACGCAGAATGAGAAGCTTCAAAAAGAGAGAGATGAGTTTCAACGAATGGTGATTGCTAACCAGCAAGTCAAGACCCAACAATTACATGAAACAAAAAAGAAAGAAAAAGAGTACATAAAGTTACAGGAGAGGTTAAACCAAGTGTTGATGGAGAAAAAGAAGGAAACGAGATCAGGCATGGAGATTATGAATCTACTCCAGAAAGAAGGGAGACAGCGTGGAACATGGAGTGGGAAGAAAACTGACTCTGATTTCTACAAAAAAATTGTGGATGCATATGAGGCGAAAAATCAAGAACTGATGGCAGAGAACACTGATCTGAGAGCGTTACTACGATCAACGCAGGGTGACATGCGTTCTTTCTTGAATGCTTCTGGTGGATTAACCAACCAATCTTTGGTAGCTAATGGAAGACACGGTGCAGACCCTTCTCAGTCTCCTTTGGGCGGGAAGACGGACGTTTTTGACCTACCTTTTCGTATGGCACGAGGTCAAATAGAAGATAGTTTACGCTCTAAGATGGTTTCCATCAAGGAACGTATGGGTCAGTTAGTAGATGCACAAAAAGAAGTATCCATTACTTCAGAAGCGTCAGAGAGGGAACTTGAACTTGAAGCACAACTCGTCGAGGCACGCAGCATTATTCAAGAACAGGAATCTATAATGTCCAAACATTTACCTAAGACAGATCGGAGAAGGAACTCAGCTCCTCCACATTCTTCTGTCAACGGACTGCGTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04520 map04520
Pfam Domains
Protein Families
ADIP

Protein Analysis

382

Amino Acids

43.51

Weight (kDa)

8.78

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADIP PF11559 35 - 182 1.6e-47 Afadin- and alpha -actinin-Binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 47, 802
AccI GTMKAC 1 cut(s) 213
AccII CGCG 1 cut(s) 86
AciI CCGC 3 cut(s) 86, 395, 852
AclWI GGATC 1 cut(s) 173
AcsI RAATTY 1 cut(s) 248
AcuI CTGAAG 1 cut(s) 972
AcyI GRCGYC 1 cut(s) 38
AfaI GTAC 2 cut(s) 15, 518
AfiI CCNNNNNNNGG 3 cut(s) 47, 802, 851
AgsI TTSAA 9 cut(s) 124, 227, 320, 425, 446, 772, 1013, 1019, 1052
AjuI GAANNNNNNNTTGG 2 cut(s) 342, 374
AluBI AGCT 5 cut(s) 132, 344, 421, 809, 1112
AluI AGCT 5 cut(s) 132, 344, 421, 809, 1112
Alw26I GTCTC 5 cut(s) 268, 295, 323, 610, 846
AlwI GGATC 1 cut(s) 173
ApeKI GCWGC 1 cut(s) 1041
ApoI RAATTY 1 cut(s) 248
AsuHPI GGTGA 5 cut(s) 54, 130, 379, 466, 764
BarI GAAGNNNNNNTAC 2 cut(s) 897, 929
BauI CACGAG 1 cut(s) 891
BbsI GAAGAC 2 cut(s) 823, 863
BbvI GCAGC 1 cut(s) 1053
BccI CCATC 4 cut(s) 547, 700, 919, 941
BciVI GTATCC 1 cut(s) 988
BclI TGATCA 1 cut(s) 268
BcoDI GTCTC 5 cut(s) 268, 295, 323, 610, 846
BfaI CTAG 1 cut(s) 195
BfuI GTATCC 1 cut(s) 988
BisI GCNGC 2 cut(s) 396, 1042
BlsI GCNGC 2 cut(s) 397, 1043
BmsI GCATC 2 cut(s) 667, 952
BpiI GAAGAC 2 cut(s) 823, 863
BpmI CTGGAG 2 cut(s) 122, 587
BpuEI CTTGAG 1 cut(s) 347
BsaHI GRCGYC 1 cut(s) 38
BsaWI WCCGGW 1 cut(s) 16
BsaXI ACNNNNNCTCC 2 cut(s) 607, 637
Bsc4I CCNNNNNNNGG 3 cut(s) 47, 802, 851
BseGI GGATG 2 cut(s) 94, 682
BseLI CCNNNNNNNGG 3 cut(s) 47, 802, 851
BseMII CTCAG 3 cut(s) 716, 851, 1122
BseRI GAGGAG 1 cut(s) 1104
BseXI GCAGC 1 cut(s) 1053
BsgI GTGCAG 1 cut(s) 846
Bsh1236I CGCG 1 cut(s) 86
Bsh1285I CGRYCG 1 cut(s) 69
BsiEI CGRYCG 1 cut(s) 69
BsiSI CCGG 1 cut(s) 17
BslI CCNNNNNNNGG 3 cut(s) 47, 802, 851
BsmAI GTCTC 5 cut(s) 268, 295, 323, 610, 846
BsmI GAATGC 1 cut(s) 778
Bsp143I GATC 7 cut(s) 66, 178, 268, 575, 721, 740, 1093
BspACI CCGC 3 cut(s) 86, 395, 852
BspCNI CTCAG 3 cut(s) 717, 850, 1121
BspFNI CGCG 1 cut(s) 86
BspPI GGATC 1 cut(s) 173
BssMI GATC 7 cut(s) 66, 178, 268, 575, 721, 740, 1093
BssNAI GTATAC 1 cut(s) 214
BssNI GRCGYC 1 cut(s) 38
BssSI CACGAG 1 cut(s) 891
Bst1107I GTATAC 1 cut(s) 214
Bst2BI CACGAG 1 cut(s) 891
Bst4CI ACNGT 5 cut(s) 13, 45, 60, 110, 824
Bst6I CTCTTC 1 cut(s) 173
BstACI GRCGYC 1 cut(s) 38
BstC8I GCNNGC 2 cut(s) 342, 1039
BstDEI CTNAG 6 cut(s) 133, 725, 837, 921, 1086, 1108
BstF5I GGATG 2 cut(s) 94, 682
BstFNI CGCG 1 cut(s) 86
BstKTI GATC 7 cut(s) 69, 181, 271, 578, 724, 743, 1096
BstMAI GTCTC 5 cut(s) 268, 295, 323, 610, 846
BstMBI GATC 7 cut(s) 66, 178, 268, 575, 721, 740, 1093
BstMCI CGRYCG 1 cut(s) 69
BstMWI GCNNNNNNNGC 1 cut(s) 686
BstNSI RCATGY 1 cut(s) 760
BstUI CGCG 1 cut(s) 86
BstV1I GCAGC 1 cut(s) 1053
BstV2I GAAGAC 2 cut(s) 823, 863
BstZ17I GTATAC 1 cut(s) 214
BsuI GTATCC 1 cut(s) 988
BtsCI GGATG 2 cut(s) 94, 682
BtsIMutI CAGTG 1 cut(s) 717
Cac8I GCNNGC 2 cut(s) 342, 1039
CseI GACGC 3 cut(s) 46, 415, 984
Csp6I GTAC 2 cut(s) 14, 517
CviAII CATG 4 cut(s) 491, 583, 630, 757
CviJI RGCY 8 cut(s) 132, 325, 344, 398, 421, 809, 1112, 1146
CviKI_1 RGCY 8 cut(s) 132, 325, 344, 398, 421, 809, 1112, 1146
CviQI GTAC 2 cut(s) 14, 517
DdeI CTNAG 6 cut(s) 133, 725, 837, 921, 1086, 1108
DpnI GATC 7 cut(s) 68, 180, 270, 577, 723, 742, 1095
DpnII GATC 7 cut(s) 66, 178, 268, 575, 721, 740, 1093
DraI TTTAAA 1 cut(s) 402
Eam1104I CTCTTC 1 cut(s) 173
EarI CTCTTC 1 cut(s) 173
Eco57I CTGAAG 1 cut(s) 972
EcoT22I ATGCAT 1 cut(s) 682
FaeI CATG 4 cut(s) 494, 586, 633, 760
FatI CATG 4 cut(s) 490, 582, 629, 756
FauI CCCGC 1 cut(s) 845
FauNDI CATATG 1 cut(s) 682
FbaI TGATCA 1 cut(s) 268
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 2 cut(s) 396, 1042
FokI GGATG 2 cut(s) 101, 689
Fsp4HI GCNGC 2 cut(s) 396, 1042
FspBI CTAG 1 cut(s) 195
GluI GCNGC 2 cut(s) 396, 1042
GsuI CTGGAG 2 cut(s) 122, 587
HapII CCGG 1 cut(s) 17
HgaI GACGC 3 cut(s) 46, 415, 984
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 4 cut(s) 494, 586, 633, 760
HincII GTYRAC 1 cut(s) 1132
HindII GTYRAC 1 cut(s) 1132
HindIII AAGCTT 1 cut(s) 419
HinfI GANTC 9 cut(s) 124, 147, 223, 257, 303, 331, 595, 650, 1061
HpaII CCGG 1 cut(s) 17
HphI GGTGA 5 cut(s) 54, 130, 379, 466, 764
Hpy166II GTNNAC 3 cut(s) 214, 914, 1132
Hpy188I TCNGA 9 cut(s) 32, 129, 273, 292, 655, 726, 991, 1000, 1098
Hpy188III TCNNGA 7 cut(s) 101, 144, 475, 604, 698, 769, 1052
Hpy8I GTNNAC 3 cut(s) 214, 914, 1132
Hpy99I CGWCG 1 cut(s) 1034
HpyAV CCTTC 4 cut(s) 559, 605, 843, 1095
HpyCH4III ACNGT 5 cut(s) 13, 45, 60, 110, 824
HpyCH4IV ACGT 2 cut(s) 864, 943
HpyCH4V TGCA 5 cut(s) 204, 340, 680, 827, 965
HpyF10VI GCNNNNNNNGC 1 cut(s) 686
HpyF3I CTNAG 6 cut(s) 133, 725, 837, 921, 1086, 1108
HpySE526I ACGT 2 cut(s) 864, 943
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 4 cut(s) 494, 586, 633, 760
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 7 cut(s) 66, 178, 268, 575, 721, 740, 1093
LmnI GCTCC 2 cut(s) 73, 1117
Lsp1109I GCAGC 1 cut(s) 1053
LweI GCATC 2 cut(s) 667, 952
MaeI CTAG 1 cut(s) 195
MaeII ACGT 2 cut(s) 864, 943
MaeIII GTNAC 6 cut(s) 60, 136, 367, 525, 732, 752
MalI GATC 7 cut(s) 68, 180, 270, 577, 723, 742, 1095
MboI GATC 7 cut(s) 66, 178, 268, 575, 721, 740, 1093
MboII GAAGA 6 cut(s) 160, 652, 828, 868, 917, 1116
MfeI CAATTG 2 cut(s) 205, 335
MluCI AATT 6 cut(s) 79, 205, 248, 335, 485, 669
MlyI GAGTC 3 cut(s) 297, 325, 644
MnlI CCTC 9 cut(s) 151, 176, 225, 528, 679, 887, 996, 1027, 1125
Mph1103I ATGCAT 1 cut(s) 682
MseI TTAA 4 cut(s) 114, 401, 539, 788
MspA1I CMGCKG 1 cut(s) 395
MspI CCGG 1 cut(s) 17
MunI CAATTG 2 cut(s) 205, 335
Mva1269I GAATGC 1 cut(s) 778
MvnI CGCG 1 cut(s) 86
MwoI GCNNNNNNNGC 1 cut(s) 686
NdeI CATATG 1 cut(s) 682
NdeII GATC 7 cut(s) 66, 178, 268, 575, 721, 740, 1093
NlaIII CATG 4 cut(s) 494, 586, 633, 760
NmuCI GTSAC 4 cut(s) 60, 136, 367, 752
NsiI ATGCAT 1 cut(s) 682
NspI RCATGY 1 cut(s) 760
PcsI WCGNNNNNNNCGW 1 cut(s) 889
PctI GAATGC 1 cut(s) 778
PfeI GAWTC 6 cut(s) 124, 147, 223, 257, 595, 1061
PflMI CCANNNNNTGG 2 cut(s) 47, 802
PkrI GCNGC 2 cut(s) 397, 1043
Ple19I CGATCG 1 cut(s) 69
PleI GAGTC 3 cut(s) 297, 325, 644
PpsI GAGTC 3 cut(s) 297, 325, 644
PvuI CGATCG 1 cut(s) 69
RsaI GTAC 2 cut(s) 15, 518
RsaNI GTAC 2 cut(s) 14, 517
SaqAI TTAA 4 cut(s) 114, 401, 539, 788
SatI GCNGC 2 cut(s) 396, 1042
Sau3AI GATC 7 cut(s) 66, 178, 268, 575, 721, 740, 1093
SchI GAGTC 3 cut(s) 297, 325, 644
SfaNI GCATC 2 cut(s) 667, 952
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
Sse9I AATT 6 cut(s) 79, 205, 248, 335, 485, 669
SsiI CCGC 3 cut(s) 86, 395, 852
SspMI CTAG 1 cut(s) 195
TaaI ACNGT 5 cut(s) 13, 45, 60, 110, 824
TaiI ACGT 2 cut(s) 867, 946
TaqI TCGA 4 cut(s) 24, 188, 306, 1032
TasI AATT 6 cut(s) 79, 205, 248, 335, 485, 669
TatI WGTACW 1 cut(s) 516
TauI GCSGC 1 cut(s) 398
TfiI GAWTC 6 cut(s) 124, 147, 223, 257, 595, 1061
Tru1I TTAA 4 cut(s) 114, 401, 539, 788
Tru9I TTAA 4 cut(s) 114, 401, 539, 788
TscAI CASTG 1 cut(s) 724
TseFI GTSAC 4 cut(s) 60, 136, 367, 752
TseI GCWGC 1 cut(s) 1041
Tsp45I GTSAC 4 cut(s) 60, 136, 367, 752
TspDTI ATGAA 2 cut(s) 507, 608
TspGWI ACGGA 3 cut(s) 392, 875, 1149
TspRI CASTG 1 cut(s) 724
Van91I CCANNNNNTGG 2 cut(s) 47, 802
XapI RAATTY 1 cut(s) 248
XceI RCATGY 1 cut(s) 760
XcmI CCANNNNNNNNNTGG 1 cut(s) 799
XmiI GTMKAC 1 cut(s) 213
XspI CTAG 1 cut(s) 195
Zsp2I ATGCAT 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.