MD16G1257500.v1.1

Afadin- and alpha-actinin-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006767
Physical Location & Seq
Reverse (-)
30161767 .. 30168802
7036 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1257500.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGGCAGCGACGGATGCAGACTTCCATCTTAGACCGTCGCCTCAATCTGCCATCCCAAACGGTGAGTTCGCATTTGCCGATGTGGGGAATTTGGAGCATTGCATTAAGTATTTGAATAAGACGCTTGTTACCTTCGGATTCCCGGCGTCGCTCGATCTCTTCGCAAAGGATCCGGTTTCTGTTGCTCGGACTTGCAATTGTATGTATGCCTTGGTTCAGCAGCGACAAAGGGATGTTGAATTTCGAGAGTCCGCTAACGAACAGAGACAGCGGCTATTGTCAGACATATCAAGATTAGAAGCTAAAGTTGAGAGGCTTGAGGGGCAGTTACAAGTCAAAGACAGGGAGATAGCAACCATTACACGAACGGAAGCTAAAGCTACAGCAGCTTTTAAATCCCAAATTGAGAAGCTACAGCAGGAGCGAGATGAGTTTCAGAGGATGGTCATTGGCAATCAGCAAGTTAGGACTCAACAAATGCATGAGATGAAGAAAAAAGAAAAGGAGTACATAAAGTTGCAGGAGAGGCTAAACCAAGTGTTGATGGAGAAAAAGAAGGAATCTAGATCAGGCATGGAGATAATGAATTTGCTTCAGAAAGAAGGGAGGCAGCGTGGAACATGGAATGGAAAGAGGGCTGATAATGATTTTCATAAAAAGATTGTGGATGCTTATGAGGCAAAAAATCAAGAATTAATGGCAGAGAATTCCGATTTAAAGGCATTATTACGATCCATGCAGGTTGATATGCGTGATTTCATAAATGCTCCAAATGGGCTGTCCAAGCAGTCCCGTGATGTAAATGAACGAGTTGAAACTGACCACACCCAGTCTCCACTGGGTGGGAGGACGGATGTCTTTGACTTGCCTTTTCACATGGCTAGAGATCAAATAGAAGAAAATCTTCGAAATAAGATGGCTTCCATAAAGGAGCGTATGGTTCAATTGCAAGATGCACAAAAAGAAGCAGAGGTTACATCTGAAGTAACCGAGAGGGAGCTTGAGCTTGAAGCTCAACTTGTCGAGGCAAGGAGCATAATTCAAGAGCAGGCATCCATAATGTCTAAACAATTGGCCAAGTCCGAGAGGCCAAGGGATTCTAATATTTCATCACCTGCTGAGGGGGTAAGAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04520 map04520
Pfam Domains
Protein Families
ADIP

Protein Analysis

379

Amino Acids

43.53

Weight (kDa)

6.4

Isoelectric Point (pI)

51.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADIP PF11559 32 - 179 2e-46 Afadin- and alpha -actinin-Binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1123
Acc36I ACCTGC 2 cut(s) 730, 1123
AccB7I CCANNNNNTGG 1 cut(s) 842
AciI CCGC 2 cut(s) 252, 271
AclWI GGATC 3 cut(s) 164, 177, 726
AcoI YGGCCR 1 cut(s) 1074
AcsI RAATTY 4 cut(s) 88, 239, 586, 706
AcuI CTGAAG 2 cut(s) 578, 1002
AcyI GRCGYC 1 cut(s) 146
AdeI CACNNNGTG 1 cut(s) 842
AfaI GTAC 1 cut(s) 509
AfiI CCNNNNNNNGG 3 cut(s) 84, 842, 1121
AgsI TTSAA 6 cut(s) 115, 239, 815, 944, 1010, 1043
AluBI AGCT 8 cut(s) 302, 374, 380, 389, 412, 1000, 1006, 1013
AluI AGCT 8 cut(s) 302, 374, 380, 389, 412, 1000, 1006, 1013
Alw26I GTCTC 2 cut(s) 259, 837
AlwI GGATC 3 cut(s) 164, 177, 726
AoxI GGCC 2 cut(s) 1074, 1088
ApeKI GCWGC 4 cut(s) 5, 220, 386, 610
ApoI RAATTY 4 cut(s) 88, 239, 586, 706
ArsI GACNNNNNNTTYG 2 cut(s) 764, 796
AseI ATTAAT 1 cut(s) 695
Asp700I GAANNNNTTC 1 cut(s) 903
AsuC2I CCSGG 1 cut(s) 143
AsuHPI GGTGA 2 cut(s) 74, 1104
AsuII TTCGAA 1 cut(s) 907
BalI TGGCCA 1 cut(s) 1076
BamHI GGATCC 1 cut(s) 169
BbvCI CCTCAGC 1 cut(s) 1119
BbvI GCAGC 4 cut(s) 17, 232, 398, 622
BccI CCATC 5 cut(s) 33, 59, 436, 538, 910
BcnI CCSGG 1 cut(s) 143
BcoDI GTCTC 2 cut(s) 259, 837
BfaI CTAG 2 cut(s) 564, 882
BfmI CTRYAG 2 cut(s) 381, 413
BfuAI ACCTGC 2 cut(s) 730, 1123
BisI GCNGC 5 cut(s) 6, 221, 272, 387, 611
BlsI GCNGC 5 cut(s) 7, 222, 273, 388, 612
Bme1390I CCNGG 1 cut(s) 143
BmiI GGNNCC 1 cut(s) 171
BmrFI CCNGG 1 cut(s) 143
BmrI ACTGGG 2 cut(s) 823, 848
BmsI GCATC 4 cut(s) 4, 658, 943, 1061
BmuI ACTGGG 2 cut(s) 823, 848
BoxI GACNNNNGTC 1 cut(s) 854
Bpu10I CCTNAGC 1 cut(s) 1119
Bpu14I TTCGAA 1 cut(s) 907
BpuEI CTTGAG 2 cut(s) 338, 1022
BpuMI CCSGG 1 cut(s) 143
BsaHI GRCGYC 1 cut(s) 146
BsaJI CCNNGG 2 cut(s) 210, 1091
BsaWI WCCGGW 1 cut(s) 172
BsaXI ACNNNNNCTCC 4 cut(s) 598, 628, 817, 847
Bsc4I CCNNNNNNNGG 3 cut(s) 84, 842, 1121
Bse1I ACTGG 2 cut(s) 829, 843
Bse3DI GCAATG 1 cut(s) 97
BseDI CCNNGG 2 cut(s) 210, 1091
BseGI GGATG 7 cut(s) 19, 51, 238, 447, 673, 859, 1052
BseLI CCNNNNNNNGG 3 cut(s) 84, 842, 1121
BseMI GCAATG 1 cut(s) 97
BseMII CTCAG 1 cut(s) 1110
BseNI ACTGG 2 cut(s) 829, 843
BseXI GCAGC 4 cut(s) 17, 232, 398, 622
BshFI GGCC 2 cut(s) 1076, 1090
BsiSI CCGG 2 cut(s) 143, 173
BslFI GGGAC 1 cut(s) 775
BslI CCNNNNNNNGG 3 cut(s) 84, 842, 1121
BsmAI GTCTC 2 cut(s) 259, 837
BsmFI GGGAC 1 cut(s) 775
BsnI GGCC 2 cut(s) 1076, 1090
Bsp119I TTCGAA 1 cut(s) 907
Bsp143I GATC 5 cut(s) 154, 169, 566, 731, 886
BspACI CCGC 2 cut(s) 252, 271
BspANI GGCC 2 cut(s) 1076, 1090
BspCNI CTCAG 1 cut(s) 1111
BspLI GGNNCC 1 cut(s) 171
BspMI ACCTGC 2 cut(s) 730, 1123
BspPI GGATC 3 cut(s) 164, 177, 726
BspT104I TTCGAA 1 cut(s) 907
BsrDI GCAATG 1 cut(s) 97
BsrI ACTGG 2 cut(s) 829, 843
BssECI CCNNGG 2 cut(s) 210, 1091
BssMI GATC 5 cut(s) 154, 169, 566, 731, 886
BssNI GRCGYC 1 cut(s) 146
BssT1I CCWWGG 2 cut(s) 210, 1091
Bst4CI ACNGT 2 cut(s) 36, 62
Bst6I CTCTTC 1 cut(s) 164
BstACI GRCGYC 1 cut(s) 146
BstBI TTCGAA 1 cut(s) 907
BstC8I GCNNGC 1 cut(s) 1050
BstDEI CTNAG 2 cut(s) 29, 1119
BstF5I GGATG 7 cut(s) 19, 51, 238, 447, 673, 859, 1052
BstKTI GATC 5 cut(s) 157, 172, 569, 734, 889
BstMAI GTCTC 2 cut(s) 259, 837
BstMBI GATC 5 cut(s) 154, 169, 566, 731, 886
BstMWI GCNNNNNNNGC 6 cut(s) 14, 322, 386, 526, 677, 784
BstPAI GACNNNNGTC 1 cut(s) 854
BstSCI CCNGG 1 cut(s) 141
BstSFI CTRYAG 2 cut(s) 381, 413
BstV1I GCAGC 4 cut(s) 17, 232, 398, 622
BstX2I RGATCY 1 cut(s) 169
BstYI RGATCY 1 cut(s) 169
BsuRI GGCC 2 cut(s) 1076, 1090
BtsCI GGATG 7 cut(s) 19, 51, 238, 447, 673, 859, 1052
BtsIMutI CAGTG 1 cut(s) 836
BveI ACCTGC 2 cut(s) 730, 1123
Cac8I GCNNGC 1 cut(s) 1050
CseI GACGC 2 cut(s) 130, 135
Csp6I GTAC 1 cut(s) 508
CviAII CATG 5 cut(s) 482, 574, 621, 736, 877
CviQI GTAC 1 cut(s) 508
DdeI CTNAG 2 cut(s) 29, 1119
DpnI GATC 5 cut(s) 156, 171, 568, 733, 888
DpnII GATC 5 cut(s) 154, 169, 566, 731, 886
DraI TTTAAA 2 cut(s) 394, 717
DraIII CACNNNGTG 1 cut(s) 842
EaeI YGGCCR 1 cut(s) 1074
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
Eco130I CCWWGG 2 cut(s) 210, 1091
Eco57I CTGAAG 2 cut(s) 578, 1002
EcoRI GAATTC 1 cut(s) 706
EcoT14I CCWWGG 2 cut(s) 210, 1091
EcoT22I ATGCAT 1 cut(s) 483
ErhI CCWWGG 2 cut(s) 210, 1091
FaeI CATG 5 cut(s) 485, 577, 624, 739, 880
FalI AAGNNNNNCTT 4 cut(s) 888, 920, 1002, 1034
FaqI GGGAC 1 cut(s) 775
FatI CATG 5 cut(s) 481, 573, 620, 735, 876
Fnu4HI GCNGC 5 cut(s) 6, 221, 272, 387, 611
FokI GGATG 7 cut(s) 26, 38, 245, 454, 680, 866, 1039
Fsp4HI GCNGC 5 cut(s) 6, 221, 272, 387, 611
FspBI CTAG 2 cut(s) 564, 882
GluI GCNGC 5 cut(s) 6, 221, 272, 387, 611
HaeIII GGCC 2 cut(s) 1076, 1090
HapII CCGG 2 cut(s) 143, 173
HgaI GACGC 2 cut(s) 130, 135
Hin1I GRCGYC 1 cut(s) 146
Hin1II CATG 5 cut(s) 485, 577, 624, 739, 880
HinfI GANTC 5 cut(s) 138, 248, 469, 560, 1097
HpaII CCGG 2 cut(s) 143, 173
HphI GGTGA 2 cut(s) 74, 1104
Hpy188I TCNGA 8 cut(s) 137, 189, 283, 438, 597, 712, 982, 1084
Hpy188III TCNNGA 5 cut(s) 245, 291, 564, 689, 1043
Hpy99I CGWCG 3 cut(s) 13, 40, 151
HpyAV CCTTC 3 cut(s) 142, 550, 596
HpyCH4III ACNGT 2 cut(s) 36, 62
HpyCH4V TGCA 8 cut(s) 17, 102, 195, 481, 520, 739, 949, 956
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 322, 386, 526, 677, 784
HpyF3I CTNAG 2 cut(s) 29, 1119
Hsp92I GRCGYC 1 cut(s) 146
Hsp92II CATG 5 cut(s) 485, 577, 624, 739, 880
Kzo9I GATC 5 cut(s) 154, 169, 566, 731, 886
LmnI GCTCC 6 cut(s) 94, 421, 772, 931, 997, 1032
Lsp1109I GCAGC 4 cut(s) 17, 232, 398, 622
LweI GCATC 4 cut(s) 4, 658, 943, 1061
MaeI CTAG 2 cut(s) 564, 882
MaeIII GTNAC 4 cut(s) 127, 327, 973, 985
MalI GATC 5 cut(s) 156, 171, 568, 733, 888
MboI GATC 5 cut(s) 154, 169, 566, 731, 886
MboII GAAGA 4 cut(s) 151, 502, 896, 908
MfeI CAATTG 3 cut(s) 196, 944, 1070
MflI RGATCY 1 cut(s) 169
MlsI TGGCCA 1 cut(s) 1076
MluNI TGGCCA 1 cut(s) 1076
MlyI GAGTC 2 cut(s) 257, 463
Mox20I TGGCCA 1 cut(s) 1076
Mph1103I ATGCAT 1 cut(s) 483
MroXI GAANNNNTTC 1 cut(s) 903
MscI TGGCCA 1 cut(s) 1076
MseI TTAA 5 cut(s) 105, 393, 695, 716, 1135
Msp20I TGGCCA 1 cut(s) 1076
MspA1I CMGCKG 1 cut(s) 271
MspI CCGG 2 cut(s) 143, 173
MspR9I CCNGG 1 cut(s) 143
MunI CAATTG 3 cut(s) 196, 944, 1070
MwoI GCNNNNNNNGC 6 cut(s) 14, 322, 386, 526, 677, 784
NciI CCSGG 1 cut(s) 143
NdeII GATC 5 cut(s) 154, 169, 566, 731, 886
NlaIII CATG 5 cut(s) 485, 577, 624, 739, 880
NlaIV GGNNCC 1 cut(s) 171
NsiI ATGCAT 1 cut(s) 483
NspV TTCGAA 1 cut(s) 907
PaqCI CACCTGC 1 cut(s) 1123
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PdmI GAANNNNTTC 1 cut(s) 903
PfeI GAWTC 3 cut(s) 138, 560, 1097
PflMI CCANNNNNTGG 1 cut(s) 842
PkrI GCNGC 5 cut(s) 7, 222, 273, 388, 612
PleI GAGTC 2 cut(s) 256, 463
PpsI GAGTC 2 cut(s) 256, 463
PshAI GACNNNNGTC 1 cut(s) 854
PshBI ATTAAT 1 cut(s) 695
PspN4I GGNNCC 1 cut(s) 171
PsuI RGATCY 1 cut(s) 169
RsaI GTAC 1 cut(s) 509
RsaNI GTAC 1 cut(s) 508
SaqAI TTAA 5 cut(s) 105, 393, 695, 716, 1135
SatI GCNGC 5 cut(s) 6, 221, 272, 387, 611
Sau3AI GATC 5 cut(s) 154, 169, 566, 731, 886
SchI GAGTC 2 cut(s) 257, 463
ScrFI CCNGG 1 cut(s) 143
SfaNI GCATC 4 cut(s) 4, 658, 943, 1061
SfcI CTRYAG 2 cut(s) 381, 413
SfuI TTCGAA 1 cut(s) 907
SmlI CTYRAG 2 cut(s) 317, 1001
SmoI CTYRAG 2 cut(s) 317, 1001
SsiI CCGC 2 cut(s) 252, 271
SspI AATATT 1 cut(s) 1105
SspMI CTAG 2 cut(s) 564, 882
StyD4I CCNGG 1 cut(s) 141
StyI CCWWGG 2 cut(s) 210, 1091
TaaI ACNGT 2 cut(s) 36, 62
TaqI TCGA 4 cut(s) 153, 244, 907, 1023
TatI WGTACW 1 cut(s) 507
TauI GCSGC 1 cut(s) 274
TfiI GAWTC 3 cut(s) 138, 560, 1097
Tru1I TTAA 5 cut(s) 105, 393, 695, 716, 1135
Tru9I TTAA 5 cut(s) 105, 393, 695, 716, 1135
TscAI CASTG 1 cut(s) 843
TseI GCWGC 4 cut(s) 5, 220, 386, 610
TspDTI ATGAA 6 cut(s) 503, 599, 641, 748, 819, 1098
TspGWI ACGGA 3 cut(s) 26, 383, 866
TspRI CASTG 1 cut(s) 843
Van91I CCANNNNNTGG 1 cut(s) 842
VspI ATTAAT 1 cut(s) 695
XapI RAATTY 4 cut(s) 88, 239, 586, 706
XbaI TCTAGA 1 cut(s) 563
XmnI GAANNNNTTC 1 cut(s) 903
XspI CTAG 2 cut(s) 564, 882
Zsp2I ATGCAT 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.