RLG00000009099

Afadin- and alpha-actinin-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0006767
Physical Location & Seq
Forward (+)
46507478 .. 46512720
5243 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009099

Sequence Viewer

Length: 1170 bp
ATGCCAGCGACTGATGCAGACTTCGATCTCAGACCTTCTCCTCAGTCCGCGTTCTCAATCGGAGAGTACGCATTTGCCGATGTGGGGAATTTGGAGCACTGTATCAAGTATTTGAACCAGACGCTCGTTACTTTCGGATTCCCGGCTTCGCTTGATCTCTTCGCCAATGATCCGGTTTCGGTAGCTCGGACTTGCAATTGTATGTATTCTTTGATTCAGCAGAGACAACGCGATGTAGAATTTCGAGAGTCTGCTAACGAACAGAGACAGCGGCTATTGTCAGACATATCAAGGTTAGAAGCTAAAGTTGAGCGGCTTGAGGGGCATATTCAAGCCAAAGATAGGGAAATAGCAACAATGACACGAACGGAAGCTAAAGCTACAGCGGCTTTTAAGTCTCAAATTGAGAAGCTGCAGAAGGAGCGAGATGAGTTTCAGAGAATGGTCATTGGGAATCAGCAAGTTAAGACTCAACAAATGCATGAGATGAAGAAAAAAGAAAAGGAGTACATAAAGCTGCAGGAGAGGCTAAACCAAGTGTTGATGGAGAAAAAGAAGGAATCTAGATCAGGCATGGAGATAATGAACTTGCTTCAGAAAGAAGGGAGGCAGCAGCGTGGAACATGGAACGGAAAGAAGACTGATAATGATTTTTATAAAAAGATTGTGGATGCTTATGAGGCCAAAAATCAAGACTTGATGGCGGAGAATTCCGATCTGAAGGCATTATTACGATCAATGCAGATGGATATGCGTGATTTCATAAATCATCCAAATGGGCAGTCTAAGCAGTCTCTAGCTGCCAATGAACGGGTTGAAACTGACCACACACAGTCTCCATTGGGTGGAAGAACGGATGTCTTTGATCTGCCCTTTCACATGGCCAGAAATCAAATAGAAGAAAGTCTCCGAAATAAGATGGCTTCGATAAAGGAGCGTATGGGTCAATTGCAAGATGCACAAAAAGAAGCAGAGGTTACTTCTGAAGCAACTGAGAGGGAGCTTGAGCTTGAAGCTCAACTTGTTGAGGCGAGGAGCATAATCCAAGAGCAGGCGTCCATAATGTCCAAACAATTTACCAAGCCTGAGAGGCAAAGGAGTCTGAATGGCCATTTTAATTCTGGCAGGGAATCTATCATTTCGTCACCTGCGGAGGGGGTAAGAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04520 map04520
Pfam Domains
Protein Families
ADIP

Protein Analysis

390

Amino Acids

44.79

Weight (kDa)

6.45

Isoelectric Point (pI)

51.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADIP PF11559 32 - 179 4.1e-47 Afadin- and alpha -actinin-Binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 657
AarI CACCTGC 1 cut(s) 1156
Acc36I ACCTGC 1 cut(s) 1156
AccB7I CCANNNNNTGG 1 cut(s) 845
AccBSI CCGCTC 1 cut(s) 313
AccII CGCG 2 cut(s) 50, 231
AciI CCGC 6 cut(s) 48, 271, 313, 386, 704, 1151
AclWI GGATC 1 cut(s) 164
AcoI YGGCCR 2 cut(s) 882, 1108
AcsI RAATTY 3 cut(s) 88, 239, 709
AcuI CTGAAG 3 cut(s) 578, 740, 1005
AcyI GRCGYC 1 cut(s) 1055
AfaI GTAC 2 cut(s) 68, 509
AfiI CCNNNNNNNGG 6 cut(s) 84, 342, 810, 845, 1051, 1154
AgsI TTSAA 4 cut(s) 115, 332, 818, 1013
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 6 cut(s) 217, 259, 402, 798, 840, 911
AlwI GGATC 1 cut(s) 164
AlwNI CAGNNNCTG 1 cut(s) 11
AoxI GGCC 3 cut(s) 681, 882, 1108
ApeKI GCWGC 5 cut(s) 412, 517, 610, 613, 800
ApoI RAATTY 3 cut(s) 88, 239, 709
ArsI GACNNNNNNTTYG 2 cut(s) 767, 799
AsuC2I CCSGG 1 cut(s) 143
AsuHPI GGTGA 1 cut(s) 1137
BalI TGGCCA 2 cut(s) 884, 1110
BbsI GAAGAC 1 cut(s) 644
Bbv12I GWGCWC 1 cut(s) 99
BbvI GCAGC 5 cut(s) 399, 504, 622, 625, 787
BccI CCATC 4 cut(s) 538, 694, 739, 913
BcnI CCSGG 1 cut(s) 143
BcoDI GTCTC 6 cut(s) 217, 259, 402, 798, 840, 911
BfaI CTAG 2 cut(s) 564, 797
BfmI CTRYAG 3 cut(s) 381, 413, 518
BfuAI ACCTGC 1 cut(s) 1156
BglI GCCNNNNNGGC 1 cut(s) 1090
BisI GCNGC 8 cut(s) 272, 314, 387, 413, 518, 611, 614, 801
BlsI GCNGC 8 cut(s) 273, 315, 388, 414, 519, 612, 615, 802
Bme1390I CCNGG 1 cut(s) 143
BmrFI CCNGG 1 cut(s) 143
BmsI GCATC 3 cut(s) 4, 661, 946
BpiI GAAGAC 1 cut(s) 644
BpuEI CTTGAG 2 cut(s) 338, 1025
BpuMI CCSGG 1 cut(s) 143
BsaHI GRCGYC 1 cut(s) 1055
BsaWI WCCGGW 1 cut(s) 172
BsaXI ACNNNNNCTCC 2 cut(s) 820, 850
Bsc4I CCNNNNNNNGG 6 cut(s) 84, 342, 810, 845, 1051, 1154
BseGI GGATG 3 cut(s) 676, 769, 862
BseLI CCNNNNNNNGG 6 cut(s) 84, 342, 810, 845, 1051, 1154
BseMII CTCAG 4 cut(s) 43, 56, 984, 1077
BseRI GAGGAG 2 cut(s) 30, 1048
BseXI GCAGC 5 cut(s) 399, 504, 622, 625, 787
Bsh1236I CGCG 2 cut(s) 50, 231
BshFI GGCC 3 cut(s) 683, 884, 1110
BsiHKAI GWGCWC 1 cut(s) 99
BsiSI CCGG 2 cut(s) 143, 173
BslI CCNNNNNNNGG 6 cut(s) 84, 342, 810, 845, 1051, 1154
BsmAI GTCTC 6 cut(s) 217, 259, 402, 798, 840, 911
BsnI GGCC 3 cut(s) 683, 884, 1110
Bsp1286I GDGCHC 1 cut(s) 99
Bsp143I GATC 7 cut(s) 25, 154, 169, 566, 715, 734, 865
BspACI CCGC 6 cut(s) 48, 271, 313, 386, 704, 1151
BspANI GGCC 3 cut(s) 683, 884, 1110
BspCNI CTCAG 4 cut(s) 42, 55, 985, 1078
BspFNI CGCG 2 cut(s) 50, 231
BspMAI CTGCAG 2 cut(s) 417, 522
BspMI ACCTGC 1 cut(s) 1156
BspPI GGATC 1 cut(s) 164
BsrBI CCGCTC 1 cut(s) 313
BssMI GATC 7 cut(s) 25, 154, 169, 566, 715, 734, 865
BssNI GRCGYC 1 cut(s) 1055
Bst4CI ACNGT 2 cut(s) 101, 834
Bst6I CTCTTC 1 cut(s) 164
BstACI GRCGYC 1 cut(s) 1055
BstC8I GCNNGC 2 cut(s) 6, 1053
BstDEI CTNAG 5 cut(s) 29, 42, 786, 993, 1086
BstF5I GGATG 3 cut(s) 676, 769, 862
BstFNI CGCG 2 cut(s) 50, 231
BstKTI GATC 7 cut(s) 28, 157, 172, 569, 718, 737, 868
BstMAI GTCTC 6 cut(s) 217, 259, 402, 798, 840, 911
BstMBI GATC 7 cut(s) 25, 154, 169, 566, 715, 734, 865
BstMWI GCNNNNNNNGC 8 cut(s) 14, 322, 386, 421, 526, 680, 787, 1090
BstSCI CCNGG 1 cut(s) 141
BstSFI CTRYAG 3 cut(s) 381, 413, 518
BstUI CGCG 2 cut(s) 50, 231
BstV1I GCAGC 5 cut(s) 399, 504, 622, 625, 787
BstV2I GAAGAC 1 cut(s) 644
BsuRI GGCC 3 cut(s) 683, 884, 1110
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 3 cut(s) 676, 769, 862
BtsIMutI CAGTG 1 cut(s) 97
BveI ACCTGC 1 cut(s) 1156
Cac8I GCNNGC 2 cut(s) 6, 1053
CaiI CAGNNNCTG 1 cut(s) 11
CseI GACGC 2 cut(s) 130, 1044
Csp6I GTAC 2 cut(s) 67, 508
CviAII CATG 4 cut(s) 482, 574, 624, 880
CviQI GTAC 2 cut(s) 67, 508
DdeI CTNAG 5 cut(s) 29, 42, 786, 993, 1086
DpnI GATC 7 cut(s) 27, 156, 171, 568, 717, 736, 867
DpnII GATC 7 cut(s) 25, 154, 169, 566, 715, 734, 865
EaeI YGGCCR 2 cut(s) 882, 1108
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
EciI GGCGGA 1 cut(s) 719
Eco57I CTGAAG 3 cut(s) 578, 740, 1005
EcoRI GAATTC 1 cut(s) 709
EcoT22I ATGCAT 1 cut(s) 483
FaeI CATG 4 cut(s) 485, 577, 627, 883
FalI AAGNNNNNCTT 2 cut(s) 1005, 1037
FatI CATG 4 cut(s) 481, 573, 623, 879
Fnu4HI GCNGC 8 cut(s) 272, 314, 387, 413, 518, 611, 614, 801
FokI GGATG 3 cut(s) 683, 756, 869
Fsp4HI GCNGC 8 cut(s) 272, 314, 387, 413, 518, 611, 614, 801
FspBI CTAG 2 cut(s) 564, 797
GluI GCNGC 8 cut(s) 272, 314, 387, 413, 518, 611, 614, 801
HaeIII GGCC 3 cut(s) 683, 884, 1110
HapII CCGG 2 cut(s) 143, 173
HgaI GACGC 2 cut(s) 130, 1044
Hin1I GRCGYC 1 cut(s) 1055
Hin1II CATG 4 cut(s) 485, 577, 627, 883
HinfI GANTC 8 cut(s) 138, 214, 248, 454, 469, 560, 1099, 1130
HpaII CCGG 2 cut(s) 143, 173
HphI GGTGA 1 cut(s) 1137
Hpy188III TCNNGA 3 cut(s) 245, 564, 692
HpyAV CCTTC 5 cut(s) 45, 412, 550, 596, 715
HpyCH4III ACNGT 2 cut(s) 101, 834
HpyCH4V TGCA 8 cut(s) 17, 195, 415, 481, 520, 742, 952, 959
HpyF10VI GCNNNNNNNGC 8 cut(s) 14, 322, 386, 421, 526, 680, 787, 1090
HpyF3I CTNAG 5 cut(s) 29, 42, 786, 993, 1086
Hsp92I GRCGYC 1 cut(s) 1055
Hsp92II CATG 4 cut(s) 485, 577, 627, 883
Kzo9I GATC 7 cut(s) 25, 154, 169, 566, 715, 734, 865
LmnI GCTCC 5 cut(s) 94, 421, 934, 1000, 1035
Lsp1109I GCAGC 5 cut(s) 399, 504, 622, 625, 787
LweI GCATC 3 cut(s) 4, 661, 946
MaeI CTAG 2 cut(s) 564, 797
MaeIII GTNAC 3 cut(s) 127, 976, 1143
MalI GATC 7 cut(s) 27, 156, 171, 568, 717, 736, 867
MbiI CCGCTC 1 cut(s) 313
MboI GATC 7 cut(s) 25, 154, 169, 566, 715, 734, 865
MboII GAAGA 5 cut(s) 151, 502, 649, 861, 911
MfeI CAATTG 2 cut(s) 196, 947
MhlI GDGCHC 1 cut(s) 99
MlsI TGGCCA 2 cut(s) 884, 1110
MluCI AATT 8 cut(s) 88, 196, 239, 402, 709, 947, 1073, 1117
MluNI TGGCCA 2 cut(s) 884, 1110
MlyI GAGTC 3 cut(s) 257, 463, 1108
Mox20I TGGCCA 2 cut(s) 884, 1110
Mph1103I ATGCAT 1 cut(s) 483
MscI TGGCCA 2 cut(s) 884, 1110
MseI TTAA 3 cut(s) 393, 465, 1116
MslI CAYNNNNRTG 1 cut(s) 774
Msp20I TGGCCA 2 cut(s) 884, 1110
MspA1I CMGCKG 2 cut(s) 271, 386
MspI CCGG 2 cut(s) 143, 173
MspR9I CCNGG 1 cut(s) 143
MunI CAATTG 2 cut(s) 196, 947
MvnI CGCG 2 cut(s) 50, 231
MwoI GCNNNNNNNGC 8 cut(s) 14, 322, 386, 421, 526, 680, 787, 1090
NciI CCSGG 1 cut(s) 143
NdeII GATC 7 cut(s) 25, 154, 169, 566, 715, 734, 865
NlaIII CATG 4 cut(s) 485, 577, 627, 883
NmuCI GTSAC 1 cut(s) 1143
NsiI ATGCAT 1 cut(s) 483
PaqCI CACCTGC 1 cut(s) 1156
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PfeI GAWTC 5 cut(s) 138, 214, 454, 560, 1130
PflMI CCANNNNNTGG 1 cut(s) 845
PkrI GCNGC 8 cut(s) 273, 315, 388, 414, 519, 612, 615, 802
PleI GAGTC 3 cut(s) 256, 463, 1107
PpsI GAGTC 3 cut(s) 256, 463, 1107
PsiI TTATAA 1 cut(s) 657
PstI CTGCAG 2 cut(s) 417, 522
PstNI CAGNNNCTG 1 cut(s) 11
RsaI GTAC 2 cut(s) 68, 509
RsaNI GTAC 2 cut(s) 67, 508
RseI CAYNNNNRTG 1 cut(s) 774
SaqAI TTAA 3 cut(s) 393, 465, 1116
SatI GCNGC 8 cut(s) 272, 314, 387, 413, 518, 611, 614, 801
Sau3AI GATC 7 cut(s) 25, 154, 169, 566, 715, 734, 865
SchI GAGTC 3 cut(s) 257, 463, 1108
ScrFI CCNGG 1 cut(s) 143
SduI GDGCHC 1 cut(s) 99
SfaNI GCATC 3 cut(s) 4, 661, 946
SfcI CTRYAG 3 cut(s) 381, 413, 518
SmiMI CAYNNNNRTG 1 cut(s) 774
SmlI CTYRAG 2 cut(s) 317, 1004
SmoI CTYRAG 2 cut(s) 317, 1004
Sse9I AATT 8 cut(s) 88, 196, 239, 402, 709, 947, 1073, 1117
SsiI CCGC 6 cut(s) 48, 271, 313, 386, 704, 1151
SspMI CTAG 2 cut(s) 564, 797
StyD4I CCNGG 1 cut(s) 141
TaaI ACNGT 2 cut(s) 101, 834
TaqI TCGA 3 cut(s) 24, 244, 926
TasI AATT 8 cut(s) 88, 196, 239, 402, 709, 947, 1073, 1117
TatI WGTACW 1 cut(s) 507
TauI GCSGC 3 cut(s) 274, 316, 389
TfiI GAWTC 5 cut(s) 138, 214, 454, 560, 1130
Tru1I TTAA 3 cut(s) 393, 465, 1116
Tru9I TTAA 3 cut(s) 393, 465, 1116
TscAI CASTG 1 cut(s) 104
TseFI GTSAC 1 cut(s) 1143
TseI GCWGC 5 cut(s) 412, 517, 610, 613, 800
Tsp45I GTSAC 1 cut(s) 1143
TspDTI ATGAA 4 cut(s) 503, 599, 751, 822
TspGWI ACGGA 3 cut(s) 383, 645, 869
TspRI CASTG 1 cut(s) 104
Van91I CCANNNNNTGG 1 cut(s) 845
XapI RAATTY 3 cut(s) 88, 239, 709
XbaI TCTAGA 1 cut(s) 563
XcmI CCANNNNNNNNNTGG 1 cut(s) 1118
XspI CTAG 2 cut(s) 564, 797
Zsp2I ATGCAT 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.