FvH4_4g08170

Afadin- and alpha-actinin-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006767
Physical Location & Seq
Reverse (-)
7961944 .. 7967317
5374 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g08170.t2

Sequence Viewer

Length: 1200 bp
ATGCCAGCGACTGATGCAGACTTCGATCTCAGACCTTCTCCGCAGTCCGCCTTCGCAATCGGAGAGTACGCGTTTGCCGATGTGGGGAATTTGGAGCACTGTATCAAGTACTTGAACCAGACTCTTGTTATGTTTGGATTTCCGGCTTCGCTTGATCTCTTCGCCAATGATCCGGTTTCGGTGGCTCGAACTTGCAATTGTATGTATTCTTTGATCCAGCAGAGACAGCGCGATGTAGAGTTCCGAGAGTCTGCGAACGAACAGAGGCAGCGGTTCTTGTCAGACATATCGAGATTGGAAGCTAAAGTTGAGCGGCTTGAGGCGCAGATACAGGCCAAAGATAGGCAGATAGCAACAATGACACGGACGGAAGCTAAAGCTACATCAGCTTTTAAGTCTCAAATTGAGAAGCTACAGAAAGAGCGAGATGAGTTTCAGAAGATGGTCATTGGGAATCAGCAAGTTAAGACTCAACAAATGCATGAGATGAAGAAAAAAGAAAAGGAGTACATAAAGCTGCAGGAGAGGCTAAACCAAGTGTTGATGGAGAAAAAGAAGGAATCTAGATCAGGCATGGAGATAATGAACTTGCTTCAGAAAGAAGGGAGGCAGCAGCGTGGGACATGGAATGGAAAGAAGACAGATAATGATTTTTATAAAAAGATTGTGGATGCTTATGAGGCCAAAAATCAAGACTTGATGGCAGAGAATGCTGATCTGAAGGCCTTATTACGATCAATGCAGATGGATATGCGTGATTTCATTAATCATCCAAATGGGCAGTCCAAGCAATCTCTGGCTGCCAATGAACGGGTTGAAACTGACCACACACAATCCCCATTGGGTGGAAGGACGGATGTTTTTGATCTGCCCTTTCACATGGCCAGAAATCAAATAGAAGAAAGTCTCCGAAATAAGATGGCTTCCATAAAGGAACGGATGGGTCAATTGCAAGATGCACAAAAGGAAGCAGAGGTTACTTCTGAAGCAACTGAGAGGGAACTTGAGCTTGAAGCTCAACTTGTTGAGGCGAGGAGCATAATCCAAGAGCAGGCGTCCTTAATGTCCAAAAAATTTCCCAAGCCTGAGAGGCAAAGGAGTCTGAATGGCCATTTTAATTCTGGCAGGGAATCTATCATTTCGCCATCTGCAGAGGGGGTAAGAAACCTAATGACACCACAATTGTCGAAGATTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04520 map04520
Pfam Domains
Protein Families
ADIP

Protein Analysis

400

Amino Acids

45.93

Weight (kDa)

7.68

Isoelectric Point (pI)

50.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADIP PF11559 38 - 185 2.1e-46 Afadin- and alpha -actinin-Binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 657
AccB7I CCANNNNNTGG 1 cut(s) 845
AccBSI CCGCTC 1 cut(s) 313
AccII CGCG 2 cut(s) 71, 231
AciI CCGC 4 cut(s) 41, 48, 271, 313
AclWI GGATC 2 cut(s) 164, 208
AcoI YGGCCR 2 cut(s) 882, 1108
AcsI RAATTY 2 cut(s) 88, 1073
AcuI CTGAAG 3 cut(s) 578, 740, 1005
AcyI GRCGYC 1 cut(s) 1055
AfaI GTAC 3 cut(s) 68, 110, 509
AfiI CCNNNNNNNGG 5 cut(s) 84, 342, 810, 845, 1051
AflIII ACRYGT 1 cut(s) 69
AgsI TTSAA 3 cut(s) 115, 818, 1013
AluBI AGCT 8 cut(s) 302, 374, 380, 389, 412, 517, 1009, 1016
AluI AGCT 8 cut(s) 302, 374, 380, 389, 412, 517, 1009, 1016
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 3 cut(s) 217, 402, 911
AlwI GGATC 2 cut(s) 164, 208
AlwNI CAGNNNCTG 1 cut(s) 11
AoxI GGCC 5 cut(s) 333, 681, 723, 882, 1108
ApeKI GCWGC 5 cut(s) 268, 517, 610, 613, 800
ApoI RAATTY 2 cut(s) 88, 1073
ArsI GACNNNNNNTTYG 2 cut(s) 767, 799
AseI ATTAAT 1 cut(s) 765
AspLEI GCGC 2 cut(s) 231, 325
BalI TGGCCA 2 cut(s) 884, 1110
BbsI GAAGAC 1 cut(s) 644
Bbv12I GWGCWC 1 cut(s) 99
BbvI GCAGC 5 cut(s) 280, 504, 622, 625, 787
BccI CCATC 7 cut(s) 436, 538, 694, 739, 913, 934, 1153
BcoDI GTCTC 3 cut(s) 217, 402, 911
BfaI CTAG 1 cut(s) 564
BfmI CTRYAG 3 cut(s) 413, 518, 1149
BglI GCCNNNNNGGC 1 cut(s) 1090
BisI GCNGC 6 cut(s) 269, 314, 518, 611, 614, 801
BlsI GCNGC 6 cut(s) 270, 315, 519, 612, 615, 802
BmcAI AGTACT 1 cut(s) 110
BmsI GCATC 3 cut(s) 4, 661, 946
BpiI GAAGAC 1 cut(s) 644
BpuEI CTTGAG 2 cut(s) 338, 1025
BsaHI GRCGYC 1 cut(s) 1055
BsaWI WCCGGW 1 cut(s) 172
Bsc4I CCNNNNNNNGG 5 cut(s) 84, 342, 810, 845, 1051
BseGI GGATG 4 cut(s) 676, 769, 862, 945
BseLI CCNNNNNNNGG 5 cut(s) 84, 342, 810, 845, 1051
BseMII CTCAG 3 cut(s) 43, 984, 1077
BseRI GAGGAG 1 cut(s) 1048
BseXI GCAGC 5 cut(s) 280, 504, 622, 625, 787
Bsh1236I CGCG 2 cut(s) 71, 231
BshFI GGCC 5 cut(s) 335, 683, 725, 884, 1110
BsiHKAI GWGCWC 1 cut(s) 99
BsiSI CCGG 2 cut(s) 143, 173
BslFI GGGAC 1 cut(s) 634
BslI CCNNNNNNNGG 5 cut(s) 84, 342, 810, 845, 1051
BsmAI GTCTC 3 cut(s) 217, 402, 911
BsmFI GGGAC 1 cut(s) 634
BsmI GAATGC 1 cut(s) 715
BsnI GGCC 5 cut(s) 335, 683, 725, 884, 1110
Bsp1286I GDGCHC 1 cut(s) 99
Bsp143I GATC 8 cut(s) 25, 154, 169, 213, 566, 715, 734, 865
BspACI CCGC 4 cut(s) 41, 48, 271, 313
BspANI GGCC 5 cut(s) 335, 683, 725, 884, 1110
BspCNI CTCAG 3 cut(s) 42, 985, 1078
BspFNI CGCG 2 cut(s) 71, 231
BspMAI CTGCAG 2 cut(s) 522, 1153
BspPI GGATC 2 cut(s) 164, 208
BsrBI CCGCTC 1 cut(s) 313
BssMI GATC 8 cut(s) 25, 154, 169, 213, 566, 715, 734, 865
BssNI GRCGYC 1 cut(s) 1055
Bst4CI ACNGT 1 cut(s) 101
Bst6I CTCTTC 1 cut(s) 164
BstACI GRCGYC 1 cut(s) 1055
BstAPI GCANNNNNTGC 1 cut(s) 710
BstC8I GCNNGC 2 cut(s) 6, 1053
BstDEI CTNAG 3 cut(s) 29, 993, 1086
BstF5I GGATG 4 cut(s) 676, 769, 862, 945
BstFNI CGCG 2 cut(s) 71, 231
BstHHI GCGC 2 cut(s) 231, 325
BstKTI GATC 8 cut(s) 28, 157, 172, 216, 569, 718, 737, 868
BstMAI GTCTC 3 cut(s) 217, 402, 911
BstMBI GATC 8 cut(s) 25, 154, 169, 213, 566, 715, 734, 865
BstMWI GCNNNNNNNGC 9 cut(s) 14, 226, 322, 386, 526, 680, 710, 787, 1090
BstSFI CTRYAG 3 cut(s) 413, 518, 1149
BstUI CGCG 2 cut(s) 71, 231
BstV1I GCAGC 5 cut(s) 280, 504, 622, 625, 787
BstV2I GAAGAC 1 cut(s) 644
BsuRI GGCC 5 cut(s) 335, 683, 725, 884, 1110
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 4 cut(s) 676, 769, 862, 945
BtsIMutI CAGTG 1 cut(s) 97
Cac8I GCNNGC 2 cut(s) 6, 1053
CaiI CAGNNNCTG 1 cut(s) 11
CfoI GCGC 2 cut(s) 231, 325
CseI GACGC 1 cut(s) 1044
Csp6I GTAC 3 cut(s) 67, 109, 508
CviAII CATG 4 cut(s) 482, 574, 624, 880
CviQI GTAC 3 cut(s) 67, 109, 508
DdeI CTNAG 3 cut(s) 29, 993, 1086
DpnI GATC 8 cut(s) 27, 156, 171, 215, 568, 717, 736, 867
DpnII GATC 8 cut(s) 25, 154, 169, 213, 566, 715, 734, 865
EaeI YGGCCR 2 cut(s) 882, 1108
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
EciI GGCGGA 1 cut(s) 37
Eco147I AGGCCT 1 cut(s) 725
Eco57I CTGAAG 3 cut(s) 578, 740, 1005
EcoT22I ATGCAT 1 cut(s) 483
FaeI CATG 4 cut(s) 485, 577, 627, 883
FalI AAGNNNNNCTT 2 cut(s) 1005, 1037
FaqI GGGAC 1 cut(s) 634
FatI CATG 4 cut(s) 481, 573, 623, 879
Fnu4HI GCNGC 6 cut(s) 269, 314, 518, 611, 614, 801
FokI GGATG 4 cut(s) 683, 756, 869, 952
Fsp4HI GCNGC 6 cut(s) 269, 314, 518, 611, 614, 801
FspBI CTAG 1 cut(s) 564
GlaI GCGC 2 cut(s) 230, 324
GluI GCNGC 6 cut(s) 269, 314, 518, 611, 614, 801
HaeIII GGCC 5 cut(s) 335, 683, 725, 884, 1110
HapII CCGG 2 cut(s) 143, 173
HgaI GACGC 1 cut(s) 1044
HhaI GCGC 2 cut(s) 231, 325
Hin1I GRCGYC 1 cut(s) 1055
Hin1II CATG 4 cut(s) 485, 577, 627, 883
Hin6I GCGC 2 cut(s) 229, 323
HinP1I GCGC 2 cut(s) 229, 323
HinfI GANTC 7 cut(s) 121, 248, 454, 469, 560, 1099, 1130
HpaII CCGG 2 cut(s) 143, 173
Hpy188III TCNNGA 3 cut(s) 291, 564, 692
HpyAV CCTTC 6 cut(s) 45, 61, 550, 596, 715, 843
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4V TGCA 8 cut(s) 17, 195, 481, 520, 742, 952, 959, 1151
HpyF10VI GCNNNNNNNGC 9 cut(s) 14, 226, 322, 386, 526, 680, 710, 787, 1090
HpyF3I CTNAG 3 cut(s) 29, 993, 1086
Hsp92I GRCGYC 1 cut(s) 1055
Hsp92II CATG 4 cut(s) 485, 577, 627, 883
HspAI GCGC 2 cut(s) 229, 323
Kzo9I GATC 8 cut(s) 25, 154, 169, 213, 566, 715, 734, 865
LmnI GCTCC 2 cut(s) 94, 1035
Lsp1109I GCAGC 5 cut(s) 280, 504, 622, 625, 787
LweI GCATC 3 cut(s) 4, 661, 946
MaeI CTAG 1 cut(s) 564
MaeIII GTNAC 1 cut(s) 976
MalI GATC 8 cut(s) 27, 156, 171, 215, 568, 717, 736, 867
MbiI CCGCTC 1 cut(s) 313
MboI GATC 8 cut(s) 25, 154, 169, 213, 566, 715, 734, 865
MboII GAAGA 5 cut(s) 151, 451, 502, 649, 911
MfeI CAATTG 3 cut(s) 196, 947, 1181
MhlI GDGCHC 1 cut(s) 99
MlsI TGGCCA 2 cut(s) 884, 1110
MluCI AATT 7 cut(s) 88, 196, 402, 947, 1073, 1117, 1181
MluI ACGCGT 1 cut(s) 69
MluNI TGGCCA 2 cut(s) 884, 1110
MlyI GAGTC 4 cut(s) 115, 257, 463, 1108
Mox20I TGGCCA 2 cut(s) 884, 1110
Mph1103I ATGCAT 1 cut(s) 483
MscI TGGCCA 2 cut(s) 884, 1110
MseI TTAA 5 cut(s) 393, 465, 765, 1061, 1116
MslI CAYNNNNRTG 1 cut(s) 774
Msp20I TGGCCA 2 cut(s) 884, 1110
MspA1I CMGCKG 1 cut(s) 271
MspI CCGG 2 cut(s) 143, 173
MunI CAATTG 3 cut(s) 196, 947, 1181
Mva1269I GAATGC 1 cut(s) 715
MvnI CGCG 2 cut(s) 71, 231
MwoI GCNNNNNNNGC 9 cut(s) 14, 226, 322, 386, 526, 680, 710, 787, 1090
NdeII GATC 8 cut(s) 25, 154, 169, 213, 566, 715, 734, 865
NlaIII CATG 4 cut(s) 485, 577, 627, 883
NsiI ATGCAT 1 cut(s) 483
PceI AGGCCT 1 cut(s) 725
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 715
PfeI GAWTC 3 cut(s) 454, 560, 1130
PflMI CCANNNNNTGG 1 cut(s) 845
PkrI GCNGC 6 cut(s) 270, 315, 519, 612, 615, 802
PleI GAGTC 4 cut(s) 115, 256, 463, 1107
PpsI GAGTC 4 cut(s) 115, 256, 463, 1107
PshBI ATTAAT 1 cut(s) 765
PsiI TTATAA 1 cut(s) 657
PstI CTGCAG 2 cut(s) 522, 1153
PstNI CAGNNNCTG 1 cut(s) 11
RsaI GTAC 3 cut(s) 68, 110, 509
RsaNI GTAC 3 cut(s) 67, 109, 508
RseI CAYNNNNRTG 1 cut(s) 774
SaqAI TTAA 5 cut(s) 393, 465, 765, 1061, 1116
SatI GCNGC 6 cut(s) 269, 314, 518, 611, 614, 801
Sau3AI GATC 8 cut(s) 25, 154, 169, 213, 566, 715, 734, 865
ScaI AGTACT 1 cut(s) 110
SchI GAGTC 4 cut(s) 115, 257, 463, 1108
SduI GDGCHC 1 cut(s) 99
SfaNI GCATC 3 cut(s) 4, 661, 946
SfcI CTRYAG 3 cut(s) 413, 518, 1149
SmiMI CAYNNNNRTG 1 cut(s) 774
SmlI CTYRAG 2 cut(s) 317, 1004
SmoI CTYRAG 2 cut(s) 317, 1004
Sse9I AATT 7 cut(s) 88, 196, 402, 947, 1073, 1117, 1181
SseBI AGGCCT 1 cut(s) 725
SsiI CCGC 4 cut(s) 41, 48, 271, 313
SspMI CTAG 1 cut(s) 564
StuI AGGCCT 1 cut(s) 725
TaaI ACNGT 1 cut(s) 101
TaqI TCGA 4 cut(s) 24, 187, 290, 1187
TasI AATT 7 cut(s) 88, 196, 402, 947, 1073, 1117, 1181
TatI WGTACW 2 cut(s) 108, 507
TauI GCSGC 1 cut(s) 316
TfiI GAWTC 3 cut(s) 454, 560, 1130
Tru1I TTAA 5 cut(s) 393, 465, 765, 1061, 1116
Tru9I TTAA 5 cut(s) 393, 465, 765, 1061, 1116
TscAI CASTG 1 cut(s) 104
TseI GCWGC 5 cut(s) 268, 517, 610, 613, 800
TspDTI ATGAA 4 cut(s) 503, 599, 751, 822
TspGWI ACGGA 4 cut(s) 379, 383, 869, 952
TspRI CASTG 1 cut(s) 104
Van91I CCANNNNNTGG 1 cut(s) 845
VspI ATTAAT 1 cut(s) 765
XapI RAATTY 2 cut(s) 88, 1073
XbaI TCTAGA 1 cut(s) 563
XcmI CCANNNNNNNNNTGG 2 cut(s) 793, 1118
XspI CTAG 1 cut(s) 564
ZrmI AGTACT 1 cut(s) 110
Zsp2I ATGCAT 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.