Rroxscaffold_5G00347030

Afadin- and alpha-actinin-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006767
Physical Location & Seq
Reverse (-)
18191986 .. 18197635
5650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00347030.1

Sequence Viewer

Length: 1041 bp
ATGCCAGCGACTGATGCAGACTTCGATCTCAGACCTTCTCCTCAGTCCGCGTTCGCAATCGGAGAGTACGCATTTGCCGATGTGGGGAATTTGGAGCACTGTATCAAGTATTTGAACCAGACGCTCGTTACTTTCGGATTCCCAGCTTCGCTTGATCTCTTCGCCAATGATCCGGTTTCGGTAGCTCGGACTTGCAATTGTATGTATTCTTTGATTCAGCAGAGACAACGCGATGTAGAATTTCGAGAGTCTGCTAACGAACAGAGACAGCGGCTATTGTCAGACATATCAAGGTTAGAAGCTAAAGTTGAGCGGCTTGAGGGGCATATTCAAGCCAAAGATAGGGAAATAGCAACAATGACACGAACGGAAGCTAAAGCTACAGCGGCTTTTAAGACTCAAATTGAGAAGCTGCAGAAAGAGCGAGATGAGTTTCAGAGAATGGTCATTGGGAATCAGCAAGTTAAGACTCAACAAATGCACGAGATGAAGAAAAAGGAAAAGGAGTACATAAAGCTGCAGGAGAGGCTAAACCAAGTGTTGATGGAGAAAAAGAAGGAATCTAGATCAGGCATGGAGATAATGAACTTGCTTCAGAAAGAAGGGAGGCAGCAGCGTGGAACATGGAATGGAAAGAAGACTGATAATGATTTTTATAAAAAGATTGTGGATGCTTATGAGGCCAAAAATCAAGACTTGATGGCGGAGAATTCCGATCTGAAGGCATTATTACGATCAATGCAGATGGATATGCGTGATTTCATAAATCATCCAAATGGGCAGTCTAAGCAGTCTCTAGCTGCCAATGAACGGGTTGAAACTGACCACACACAATCTCCATTGGGTGGAAGAACGGATGTCTTTGATCTGCCCTTTCACATGGCCAGAAATCAAATAGAAGAAAGTCTCCGAAATAAGATGGCTTCGATAAAGGAGCGTATGGGTCAATTGCAAGATGCACAAAAAGAAGCGGAGGTTACTTCTGAAGCAACTGAGAGGAGCTTGAGCTTGAAGCTCAACTTGTTGAGGCGAGGAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04520 map04520
Pfam Domains
Protein Families
ADIP

Protein Analysis

346

Amino Acids

39.93

Weight (kDa)

8.27

Isoelectric Point (pI)

50.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADIP PF11559 32 - 179 2.1e-47 Afadin- and alpha -actinin-Binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 657
AccB7I CCANNNNNTGG 1 cut(s) 845
AccBSI CCGCTC 1 cut(s) 313
AccII CGCG 2 cut(s) 50, 231
AciI CCGC 6 cut(s) 48, 271, 313, 386, 704, 971
AclWI GGATC 1 cut(s) 164
AcoI YGGCCR 1 cut(s) 882
AcsI RAATTY 3 cut(s) 88, 239, 709
AcuI CTGAAG 3 cut(s) 578, 740, 1005
AfaI GTAC 2 cut(s) 68, 509
AfiI CCNNNNNNNGG 4 cut(s) 84, 342, 810, 845
AgsI TTSAA 4 cut(s) 115, 332, 818, 1012
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 4 cut(s) 217, 259, 798, 911
AlwI GGATC 1 cut(s) 164
AlwNI CAGNNNCTG 1 cut(s) 11
AoxI GGCC 2 cut(s) 681, 882
ApeKI GCWGC 5 cut(s) 412, 517, 610, 613, 800
ApoI RAATTY 3 cut(s) 88, 239, 709
ArsI GACNNNNNNTTYG 2 cut(s) 767, 799
BalI TGGCCA 1 cut(s) 884
BauI CACGAG 1 cut(s) 482
BbsI GAAGAC 1 cut(s) 644
Bbv12I GWGCWC 1 cut(s) 99
BbvI GCAGC 5 cut(s) 399, 504, 622, 625, 787
BccI CCATC 4 cut(s) 538, 694, 739, 913
BcoDI GTCTC 4 cut(s) 217, 259, 798, 911
BfaI CTAG 2 cut(s) 564, 797
BfmI CTRYAG 3 cut(s) 381, 413, 518
BisI GCNGC 8 cut(s) 272, 314, 387, 413, 518, 611, 614, 801
BlsI GCNGC 8 cut(s) 273, 315, 388, 414, 519, 612, 615, 802
BmsI GCATC 3 cut(s) 4, 661, 946
BpiI GAAGAC 1 cut(s) 644
BpuEI CTTGAG 2 cut(s) 338, 1024
BsaWI WCCGGW 1 cut(s) 172
BsaXI ACNNNNNCTCC 2 cut(s) 820, 850
Bsc4I CCNNNNNNNGG 4 cut(s) 84, 342, 810, 845
BseGI GGATG 3 cut(s) 676, 769, 862
BseLI CCNNNNNNNGG 4 cut(s) 84, 342, 810, 845
BseMII CTCAG 3 cut(s) 43, 56, 984
BseRI GAGGAG 2 cut(s) 30, 1012
BseXI GCAGC 5 cut(s) 399, 504, 622, 625, 787
BseYI CCCAGC 1 cut(s) 142
Bsh1236I CGCG 2 cut(s) 50, 231
BshFI GGCC 2 cut(s) 683, 884
BsiHKAI GWGCWC 1 cut(s) 99
BsiSI CCGG 1 cut(s) 173
BslI CCNNNNNNNGG 4 cut(s) 84, 342, 810, 845
BsmAI GTCTC 4 cut(s) 217, 259, 798, 911
BsnI GGCC 2 cut(s) 683, 884
Bsp1286I GDGCHC 1 cut(s) 99
Bsp143I GATC 7 cut(s) 25, 154, 169, 566, 715, 734, 865
BspACI CCGC 6 cut(s) 48, 271, 313, 386, 704, 971
BspANI GGCC 2 cut(s) 683, 884
BspCNI CTCAG 3 cut(s) 42, 55, 985
BspFNI CGCG 2 cut(s) 50, 231
BspMAI CTGCAG 2 cut(s) 417, 522
BspPI GGATC 1 cut(s) 164
BsrBI CCGCTC 1 cut(s) 313
BssMI GATC 7 cut(s) 25, 154, 169, 566, 715, 734, 865
BssSI CACGAG 1 cut(s) 482
Bst2BI CACGAG 1 cut(s) 482
Bst4CI ACNGT 1 cut(s) 101
Bst6I CTCTTC 1 cut(s) 164
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 4 cut(s) 29, 42, 786, 993
BstF5I GGATG 3 cut(s) 676, 769, 862
BstFNI CGCG 2 cut(s) 50, 231
BstKTI GATC 7 cut(s) 28, 157, 172, 569, 718, 737, 868
BstMAI GTCTC 4 cut(s) 217, 259, 798, 911
BstMBI GATC 7 cut(s) 25, 154, 169, 566, 715, 734, 865
BstMWI GCNNNNNNNGC 7 cut(s) 14, 322, 386, 421, 526, 680, 787
BstSFI CTRYAG 3 cut(s) 381, 413, 518
BstUI CGCG 2 cut(s) 50, 231
BstV1I GCAGC 5 cut(s) 399, 504, 622, 625, 787
BstV2I GAAGAC 1 cut(s) 644
BsuRI GGCC 2 cut(s) 683, 884
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 3 cut(s) 676, 769, 862
BtsIMutI CAGTG 1 cut(s) 97
Cac8I GCNNGC 1 cut(s) 6
CaiI CAGNNNCTG 1 cut(s) 11
CseI GACGC 1 cut(s) 130
Csp6I GTAC 2 cut(s) 67, 508
CviAII CATG 3 cut(s) 574, 624, 880
CviQI GTAC 2 cut(s) 67, 508
DdeI CTNAG 4 cut(s) 29, 42, 786, 993
DpnI GATC 7 cut(s) 27, 156, 171, 568, 717, 736, 867
DpnII GATC 7 cut(s) 25, 154, 169, 566, 715, 734, 865
EaeI YGGCCR 1 cut(s) 882
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
EciI GGCGGA 1 cut(s) 719
Eco57I CTGAAG 3 cut(s) 578, 740, 1005
EcoRI GAATTC 1 cut(s) 709
FaeI CATG 3 cut(s) 577, 627, 883
FalI AAGNNNNNCTT 2 cut(s) 1004, 1036
FatI CATG 3 cut(s) 573, 623, 879
Fnu4HI GCNGC 8 cut(s) 272, 314, 387, 413, 518, 611, 614, 801
FokI GGATG 3 cut(s) 683, 756, 869
Fsp4HI GCNGC 8 cut(s) 272, 314, 387, 413, 518, 611, 614, 801
FspBI CTAG 2 cut(s) 564, 797
GluI GCNGC 8 cut(s) 272, 314, 387, 413, 518, 611, 614, 801
GsaI CCCAGC 1 cut(s) 146
HaeIII GGCC 2 cut(s) 683, 884
HapII CCGG 1 cut(s) 173
HgaI GACGC 1 cut(s) 130
Hin1II CATG 3 cut(s) 577, 627, 883
HinfI GANTC 7 cut(s) 138, 214, 248, 397, 454, 469, 560
HpaII CCGG 1 cut(s) 173
Hpy188III TCNNGA 3 cut(s) 245, 564, 692
HpyAV CCTTC 4 cut(s) 45, 550, 596, 715
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4V TGCA 8 cut(s) 17, 195, 415, 481, 520, 742, 952, 959
HpyF10VI GCNNNNNNNGC 7 cut(s) 14, 322, 386, 421, 526, 680, 787
HpyF3I CTNAG 4 cut(s) 29, 42, 786, 993
Hsp92II CATG 3 cut(s) 577, 627, 883
Kzo9I GATC 7 cut(s) 25, 154, 169, 566, 715, 734, 865
LmnI GCTCC 4 cut(s) 94, 934, 999, 1034
LpnPI CCDG 7 cut(s) 18, 131, 156, 186, 506, 555, 898
Lsp1109I GCAGC 5 cut(s) 399, 504, 622, 625, 787
LweI GCATC 3 cut(s) 4, 661, 946
MaeI CTAG 2 cut(s) 564, 797
MaeIII GTNAC 2 cut(s) 127, 976
MalI GATC 7 cut(s) 27, 156, 171, 568, 717, 736, 867
MbiI CCGCTC 1 cut(s) 313
MboI GATC 7 cut(s) 25, 154, 169, 566, 715, 734, 865
MboII GAAGA 5 cut(s) 151, 502, 649, 861, 911
MfeI CAATTG 2 cut(s) 196, 947
MhlI GDGCHC 1 cut(s) 99
MlsI TGGCCA 1 cut(s) 884
MluCI AATT 6 cut(s) 88, 196, 239, 402, 709, 947
MluNI TGGCCA 1 cut(s) 884
MlyI GAGTC 3 cut(s) 257, 391, 463
MnlI CCTC 9 cut(s) 51, 313, 519, 600, 673, 967, 990, 1020, 1025
Mox20I TGGCCA 1 cut(s) 884
MscI TGGCCA 1 cut(s) 884
MseI TTAA 2 cut(s) 393, 465
MslI CAYNNNNRTG 1 cut(s) 774
Msp20I TGGCCA 1 cut(s) 884
MspA1I CMGCKG 2 cut(s) 271, 386
MspI CCGG 1 cut(s) 173
MunI CAATTG 2 cut(s) 196, 947
MvnI CGCG 2 cut(s) 50, 231
MwoI GCNNNNNNNGC 7 cut(s) 14, 322, 386, 421, 526, 680, 787
NdeII GATC 7 cut(s) 25, 154, 169, 566, 715, 734, 865
NlaIII CATG 3 cut(s) 577, 627, 883
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PfeI GAWTC 4 cut(s) 138, 214, 454, 560
PflMI CCANNNNNTGG 1 cut(s) 845
PkrI GCNGC 8 cut(s) 273, 315, 388, 414, 519, 612, 615, 802
PleI GAGTC 3 cut(s) 256, 391, 463
PpsI GAGTC 3 cut(s) 256, 391, 463
PsiI TTATAA 1 cut(s) 657
PspFI CCCAGC 1 cut(s) 142
PstI CTGCAG 2 cut(s) 417, 522
PstNI CAGNNNCTG 1 cut(s) 11
RsaI GTAC 2 cut(s) 68, 509
RsaNI GTAC 2 cut(s) 67, 508
RseI CAYNNNNRTG 1 cut(s) 774
SaqAI TTAA 2 cut(s) 393, 465
SatI GCNGC 8 cut(s) 272, 314, 387, 413, 518, 611, 614, 801
Sau3AI GATC 7 cut(s) 25, 154, 169, 566, 715, 734, 865
SchI GAGTC 3 cut(s) 257, 391, 463
SduI GDGCHC 1 cut(s) 99
SfaNI GCATC 3 cut(s) 4, 661, 946
SfcI CTRYAG 3 cut(s) 381, 413, 518
SmiMI CAYNNNNRTG 1 cut(s) 774
SmlI CTYRAG 2 cut(s) 317, 1003
SmoI CTYRAG 2 cut(s) 317, 1003
Sse9I AATT 6 cut(s) 88, 196, 239, 402, 709, 947
SsiI CCGC 6 cut(s) 48, 271, 313, 386, 704, 971
SspMI CTAG 2 cut(s) 564, 797
TaaI ACNGT 1 cut(s) 101
TaqI TCGA 3 cut(s) 24, 244, 926
TasI AATT 6 cut(s) 88, 196, 239, 402, 709, 947
TatI WGTACW 1 cut(s) 507
TauI GCSGC 3 cut(s) 274, 316, 389
TfiI GAWTC 4 cut(s) 138, 214, 454, 560
Tru1I TTAA 2 cut(s) 393, 465
Tru9I TTAA 2 cut(s) 393, 465
TscAI CASTG 1 cut(s) 104
TseI GCWGC 5 cut(s) 412, 517, 610, 613, 800
TspDTI ATGAA 4 cut(s) 503, 599, 751, 822
TspGWI ACGGA 2 cut(s) 383, 869
TspRI CASTG 1 cut(s) 104
Van91I CCANNNNNTGG 1 cut(s) 845
XapI RAATTY 3 cut(s) 88, 239, 709
XbaI TCTAGA 1 cut(s) 563
XspI CTAG 2 cut(s) 564, 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.