AT5G57410

Afadin- and alpha-actinin-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006767
Physical Location & Seq
Reverse (-)
23264314 .. 23267905
3592 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G57410.2

Sequence Viewer

Length: 1122 bp
ATGCCGGCGAATGATGCTGAATACGATCTCAAAGTTTTATCTCAATCTGCGTCAGCAGGGGATTACACATTTGCGAATGAGGATAACTTGGAGCATTGTACGAAGTATTTGAATCAGACGATGGTTACATTTGGATTTCCCGCCTCGCTCGATCTCTTTTCGAATGATCCTGTTTCTATATCAAGGACCTGCAATTGTATGTACTCGTTGCTGCAGCAGCGACAACGTGATATAGAGTTTAGAGAATCTGCTAATGAGCTGAGACAGAGACAGCAATCGGATATAGCTAGACTTGAAGCTAAAGTTGAGAGGCTTGAAGCGCTACTCCAACAGAAGGACAGAGAAATTGCAACAATTACCAGAACCGAAGCCAAAAACACTGCAGCTTTGAAGTCTCAGATTGAAAAGTTACAGCAGGAGAGGGATGAATTCCAAAGGATGGTGATTGGTAACCAGCAAGTGAAGGCTCAACAGATACACGAAATGAAGAAAAAGGAGAAGGATTACATTAAGTTGCAGGAACGGCTGAATCAAGTGTTAATGGAGAAAAAGAAAGAATCAAGATCTGGAATGGAGATTATGAATTTGCTACAGAAAGAAGGGAGGCAACGTGGTACCTGGAATGGCAAGAAGACTGATACCGACTTCTATAAAAAGATAGTGGATGCGTACGAAGCGAAAAATCAAGAATTGATGGCTGAGAACACTAGTCTAAGAGCATTACTTCGATCCATGCAGACAGATATGCGTGATTTCTTAAATGCTCCAAATGGGTCAGCTACATTGGCTGGAAGTGAGAAACGTGAGGCTGATCCTTCACAATCTCCACTGGGTGGGAAGACGGACGTGTTTGATCTACCTTACCGGATGGCTAGAGGTCAAATAGAAGAAAGTTTGCGTACTAAGATGGCTTCCATAAAGGAAAGCATGGTCCAGTTACAAGATGCACCTAAAAGAGCATCTGTTACATCTGAAGCAACAGAGAGAGAGCTTGAACTCGAAGCTCAGCTTGTCGAGGCAAGAAGCATAATCCAAGAACAGGAGTCCATAATGTCGAAACATCTCCCAAAATCAGAGCAGCGAAGGGAATCTGTAGCTTCGTTGGCAGCTGAGGGAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04520 map04520
Pfam Domains
Protein Families
ADIP

Protein Analysis

373

Amino Acids

42.66

Weight (kDa)

5.94

Isoelectric Point (pI)

56.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADIP PF11559 2 - 138 6.3e-39 Afadin- and alpha -actinin-Binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 197
Acc65I GGTACC 1 cut(s) 614
AccB1I GGYRCC 1 cut(s) 614
AccB7I CCANNNNNTGG 2 cut(s) 439, 833
AciI CCGC 1 cut(s) 141
AclWI GGATC 3 cut(s) 161, 723, 806
AcsI RAATTY 2 cut(s) 428, 583
AcuI CTGAAG 1 cut(s) 993
AdeI CACNNNGTG 1 cut(s) 833
AfaI GTAC 5 cut(s) 100, 203, 616, 671, 901
AfeI AGCGCT 1 cut(s) 321
AfiI CCNNNNNNNGG 4 cut(s) 334, 439, 833, 1039
AflIII ACRYGT 1 cut(s) 846
AgsI TTSAA 6 cut(s) 112, 296, 317, 391, 404, 995
AhlI ACTAGT 1 cut(s) 707
AjiI CACGTC 1 cut(s) 847
AjnI CCWGG 1 cut(s) 617
Alw26I GTCTC 3 cut(s) 256, 262, 399
AlwI GGATC 3 cut(s) 161, 723, 806
Aor51HI AGCGCT 1 cut(s) 321
ApeKI GCWGC 6 cut(s) 211, 214, 217, 383, 1078, 1106
ApoI RAATTY 2 cut(s) 428, 583
Asp718I GGTACC 1 cut(s) 614
AspLEI GCGC 1 cut(s) 322
AspS9I GGNCC 2 cut(s) 186, 931
AsuHPI GGTGA 1 cut(s) 454
AsuII TTCGAA 1 cut(s) 161
AvaII GGWCC 2 cut(s) 186, 931
BanI GGYRCC 1 cut(s) 614
BbsI GAAGAC 2 cut(s) 638, 845
BbvCI CCTCAGC 1 cut(s) 1110
BbvI GCAGC 6 cut(s) 198, 226, 229, 395, 1090, 1118
BccI CCATC 5 cut(s) 115, 433, 688, 862, 901
BceAI ACGGC 1 cut(s) 539
BciT130I CCWGG 1 cut(s) 619
BcoDI GTCTC 3 cut(s) 256, 262, 399
BcuI ACTAGT 1 cut(s) 707
BfaI CTAG 3 cut(s) 288, 708, 873
BfmI CTRYAG 4 cut(s) 212, 381, 590, 1092
BfoI RGCGCY 1 cut(s) 323
BfuAI ACCTGC 1 cut(s) 197
BglII AGATCT 1 cut(s) 563
BisI GCNGC 6 cut(s) 212, 215, 218, 384, 1079, 1107
BlpI GCTNAGC 1 cut(s) 1005
BlsI GCNGC 6 cut(s) 213, 216, 219, 385, 1080, 1108
Bme1390I CCNGG 1 cut(s) 619
Bme18I GGWCC 2 cut(s) 186, 931
BmgBI CACGTC 1 cut(s) 847
BmgT120I GGNCC 2 cut(s) 186, 931
BmiI GGNNCC 1 cut(s) 616
BmrFI CCNGG 1 cut(s) 619
BmrI ACTGGG 1 cut(s) 839
BmsI GCATC 4 cut(s) 4, 655, 934, 968
BmuI ACTGGG 1 cut(s) 839
BpiI GAAGAC 2 cut(s) 638, 845
Bpu10I CCTNAGC 1 cut(s) 1110
Bpu1102I GCTNAGC 1 cut(s) 1005
Bpu14I TTCGAA 1 cut(s) 161
BsaWI WCCGGW 1 cut(s) 864
BsaXI ACNNNNNCTCC 2 cut(s) 595, 625
Bsc4I CCNNNNNNNGG 4 cut(s) 334, 439, 833, 1039
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 2 cut(s) 834, 934
BseBI CCWGG 1 cut(s) 619
BseGI GGATG 4 cut(s) 430, 444, 670, 873
BseLI CCNNNNNNNGG 4 cut(s) 334, 439, 833, 1039
BseMII CTCAG 5 cut(s) 251, 410, 690, 1019, 1101
BseNI ACTGG 2 cut(s) 834, 934
BseXI GCAGC 6 cut(s) 198, 226, 229, 395, 1090, 1118
BshNI GGYRCC 1 cut(s) 614
BsiSI CCGG 2 cut(s) 5, 865
BsiWI CGTACG 1 cut(s) 669
BslI CCNNNNNNNGG 4 cut(s) 334, 439, 833, 1039
BsmAI GTCTC 3 cut(s) 256, 262, 399
Bsp119I TTCGAA 1 cut(s) 161
Bsp143I GATC 7 cut(s) 25, 151, 166, 563, 728, 811, 853
Bsp1720I GCTNAGC 1 cut(s) 1005
BspACI CCGC 1 cut(s) 141
BspCNI CTCAG 5 cut(s) 252, 409, 691, 1018, 1102
BspLI GGNNCC 1 cut(s) 616
BspMAI CTGCAG 2 cut(s) 216, 385
BspMI ACCTGC 1 cut(s) 197
BspPI GGATC 3 cut(s) 161, 723, 806
BspT104I TTCGAA 1 cut(s) 161
BspT107I GGYRCC 1 cut(s) 614
BsrFI RCCGGY 1 cut(s) 4
BsrI ACTGG 2 cut(s) 834, 934
BssAI RCCGGY 1 cut(s) 4
BssMI GATC 7 cut(s) 25, 151, 166, 563, 728, 811, 853
Bst2UI CCWGG 1 cut(s) 619
BstBI TTCGAA 1 cut(s) 161
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 7 cut(s) 260, 396, 699, 713, 903, 1005, 1110
BstEII GGTNACC 1 cut(s) 449
BstF5I GGATG 4 cut(s) 430, 444, 670, 873
BstH2I RGCGCY 1 cut(s) 323
BstHHI GCGC 1 cut(s) 322
BstKTI GATC 7 cut(s) 28, 154, 169, 566, 731, 814, 856
BstMAI GTCTC 3 cut(s) 256, 262, 399
BstMBI GATC 7 cut(s) 25, 151, 166, 563, 728, 811, 853
BstMWI GCNNNNNNNGC 7 cut(s) 14, 217, 319, 523, 674, 785, 1103
BstNI CCWGG 1 cut(s) 619
BstPI GGTNACC 1 cut(s) 449
BstSCI CCNGG 1 cut(s) 617
BstSFI CTRYAG 4 cut(s) 212, 381, 590, 1092
BstV1I GCAGC 6 cut(s) 198, 226, 229, 395, 1090, 1118
BstV2I GAAGAC 2 cut(s) 638, 845
BstX2I RGATCY 1 cut(s) 563
BstYI RGATCY 1 cut(s) 563
BtrI CACGTC 1 cut(s) 847
BtsCI GGATG 4 cut(s) 430, 444, 670, 873
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 2 cut(s) 378, 827
BveI ACCTGC 1 cut(s) 197
Cac8I GCNNGC 1 cut(s) 6
CfoI GCGC 1 cut(s) 322
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 2 cut(s) 186, 931
CseI GACGC 1 cut(s) 39
Csp6I GTAC 5 cut(s) 99, 202, 615, 670, 900
CviAII CATG 2 cut(s) 733, 928
CviQI GTAC 5 cut(s) 99, 202, 615, 670, 900
DdeI CTNAG 7 cut(s) 260, 396, 699, 713, 903, 1005, 1110
DpnI GATC 7 cut(s) 27, 153, 168, 565, 730, 813, 855
DpnII GATC 7 cut(s) 25, 151, 166, 563, 728, 811, 853
DraIII CACNNNGTG 1 cut(s) 833
Eco47I GGWCC 2 cut(s) 186, 931
Eco47III AGCGCT 1 cut(s) 321
Eco57I CTGAAG 1 cut(s) 993
Eco91I GGTNACC 1 cut(s) 449
EcoO109I RGGNCCY 1 cut(s) 186
EcoO65I GGTNACC 1 cut(s) 449
EcoRI GAATTC 1 cut(s) 428
EcoRII CCWGG 1 cut(s) 617
FaeI CATG 2 cut(s) 736, 931
FalI AAGNNNNNCTT 2 cut(s) 993, 1025
FatI CATG 2 cut(s) 732, 927
FauI CCCGC 1 cut(s) 148
Fnu4HI GCNGC 6 cut(s) 212, 215, 218, 384, 1079, 1107
FokI GGATG 4 cut(s) 437, 451, 677, 880
Fsp4HI GCNGC 6 cut(s) 212, 215, 218, 384, 1079, 1107
FspBI CTAG 3 cut(s) 288, 708, 873
GlaI GCGC 1 cut(s) 321
GluI GCNGC 6 cut(s) 212, 215, 218, 384, 1079, 1107
HaeII RGCGCY 1 cut(s) 323
HapII CCGG 2 cut(s) 5, 865
HgaI GACGC 1 cut(s) 39
HhaI GCGC 1 cut(s) 322
Hin1II CATG 2 cut(s) 736, 931
Hin6I GCGC 1 cut(s) 320
HinP1I GCGC 1 cut(s) 320
HinfI GANTC 6 cut(s) 112, 245, 529, 557, 1043, 1088
HpaII CCGG 2 cut(s) 5, 865
HphI GGTGA 1 cut(s) 454
Hpy188I TCNGA 5 cut(s) 117, 280, 399, 973, 1075
Hpy188III TCNNGA 3 cut(s) 561, 567, 686
HpyAV CCTTC 6 cut(s) 328, 457, 493, 593, 825, 1077
HpyCH4IV ACGT 4 cut(s) 226, 610, 802, 846
HpyCH4V TGCA 7 cut(s) 192, 214, 350, 383, 517, 736, 947
HpyF10VI GCNNNNNNNGC 7 cut(s) 14, 217, 319, 523, 674, 785, 1103
HpyF3I CTNAG 7 cut(s) 260, 396, 699, 713, 903, 1005, 1110
HpySE526I ACGT 4 cut(s) 226, 610, 802, 846
Hsp92II CATG 2 cut(s) 736, 931
HspAI GCGC 1 cut(s) 320
KpnI GGTACC 1 cut(s) 618
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Kzo9I GATC 7 cut(s) 25, 151, 166, 563, 728, 811, 853
LmnI GCTCC 2 cut(s) 91, 769
Lsp1109I GCAGC 6 cut(s) 198, 226, 229, 395, 1090, 1118
LweI GCATC 4 cut(s) 4, 655, 934, 968
MaeI CTAG 3 cut(s) 288, 708, 873
MaeII ACGT 4 cut(s) 226, 610, 802, 846
MaeIII GTNAC 5 cut(s) 124, 408, 449, 936, 964
MalI GATC 7 cut(s) 27, 153, 168, 565, 730, 813, 855
MboI GATC 7 cut(s) 25, 151, 166, 563, 728, 811, 853
MboII GAAGA 4 cut(s) 499, 643, 850, 899
MfeI CAATTG 1 cut(s) 193
MflI RGATCY 1 cut(s) 563
MluCI AATT 6 cut(s) 193, 345, 354, 428, 583, 689
MlyI GAGTC 1 cut(s) 1052
MmeI TCCRAC 1 cut(s) 352
MnlI CCTC 9 cut(s) 73, 154, 303, 414, 597, 799, 869, 1009, 1105
MroNI GCCGGC 1 cut(s) 4
MseI TTAA 3 cut(s) 510, 539, 758
MspA1I CMGCKG 1 cut(s) 1109
MspI CCGG 2 cut(s) 5, 865
MspR9I CCNGG 1 cut(s) 619
MunI CAATTG 1 cut(s) 193
MvaI CCWGG 1 cut(s) 619
MwoI GCNNNNNNNGC 7 cut(s) 14, 217, 319, 523, 674, 785, 1103
NaeI GCCGGC 1 cut(s) 6
NdeII GATC 7 cut(s) 25, 151, 166, 563, 728, 811, 853
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 2 cut(s) 736, 931
NlaIV GGNNCC 1 cut(s) 616
NspV TTCGAA 1 cut(s) 161
PdiI GCCGGC 1 cut(s) 6
PfeI GAWTC 5 cut(s) 112, 245, 529, 557, 1088
Pfl23II CGTACG 1 cut(s) 669
PflMI CCANNNNNTGG 2 cut(s) 439, 833
PkrI GCNGC 6 cut(s) 213, 216, 219, 385, 1080, 1108
PleI GAGTC 1 cut(s) 1051
PpsI GAGTC 1 cut(s) 1051
PpuMI RGGWCCY 1 cut(s) 186
Psp5II RGGWCCY 1 cut(s) 186
Psp6I CCWGG 1 cut(s) 617
PspEI GGTNACC 1 cut(s) 449
PspGI CCWGG 1 cut(s) 617
PspLI CGTACG 1 cut(s) 669
PspN4I GGNNCC 1 cut(s) 616
PspPI GGNCC 2 cut(s) 186, 931
PspPPI RGGWCCY 1 cut(s) 186
PstI CTGCAG 2 cut(s) 216, 385
PsuI RGATCY 1 cut(s) 563
PvuII CAGCTG 1 cut(s) 1109
RsaI GTAC 5 cut(s) 100, 203, 616, 671, 901
RsaNI GTAC 5 cut(s) 99, 202, 615, 670, 900
SaqAI TTAA 3 cut(s) 510, 539, 758
SatI GCNGC 6 cut(s) 212, 215, 218, 384, 1079, 1107
Sau3AI GATC 7 cut(s) 25, 151, 166, 563, 728, 811, 853
Sau96I GGNCC 2 cut(s) 186, 931
SchI GAGTC 1 cut(s) 1052
ScrFI CCNGG 1 cut(s) 619
SfaNI GCATC 4 cut(s) 4, 655, 934, 968
SfcI CTRYAG 4 cut(s) 212, 381, 590, 1092
SfuI TTCGAA 1 cut(s) 161
SinI GGWCC 2 cut(s) 186, 931
SpeI ACTAGT 1 cut(s) 707
Sse9I AATT 6 cut(s) 193, 345, 354, 428, 583, 689
SsiI CCGC 1 cut(s) 141
SspMI CTAG 3 cut(s) 288, 708, 873
StyD4I CCNGG 1 cut(s) 617
TaiI ACGT 4 cut(s) 229, 613, 805, 849
TaqI TCGA 6 cut(s) 150, 161, 727, 999, 1014, 1055
TasI AATT 6 cut(s) 193, 345, 354, 428, 583, 689
TatI WGTACW 1 cut(s) 201
TfiI GAWTC 5 cut(s) 112, 245, 529, 557, 1088
Tru1I TTAA 3 cut(s) 510, 539, 758
Tru9I TTAA 3 cut(s) 510, 539, 758
TscAI CASTG 2 cut(s) 385, 834
TseI GCWGC 6 cut(s) 211, 214, 217, 383, 1078, 1106
TspDTI ATGAA 3 cut(s) 441, 500, 596
TspGWI ACGGA 1 cut(s) 857
TspRI CASTG 2 cut(s) 385, 834
Van91I CCANNNNNTGG 2 cut(s) 439, 833
VpaK11BI GGWCC 2 cut(s) 186, 931
XapI RAATTY 2 cut(s) 428, 583
XspI CTAG 3 cut(s) 288, 708, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.