MD13G1253000.v1.1

Afadin- and alpha-actinin-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0006767
Physical Location & Seq
Reverse (-)
27144364 .. 27150243
5880 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1253000.v1.1.491

Sequence Viewer

Length: 1170 bp
ATGCCTGCGACGGACACAGACTTCGATCTCAGACCGTTGCCTCAGTCCGCCATCCCAATCGGAGAGTTCGCATTTGCCGATGTGGGGAATTTGGAGCATTGCATTAAGTATTTGAATCAGACACTTGTTACCTTCGGATTCCCGGCGTCACTTGATCTCTTCGCAAATGATCCGGTTTCTGTTGCTCGGACTTGCAATTGTATGTATGCTTTGGTTCAGCAGAGACAAAGGGATGTTGAATTTCGAGAGTCCGCTAATGAACAGAGACAGCGGCTGGTGTCTGACATATCAAGATTAGAAGCTAAAGTTGAGAGGCTTGAGGGGCAGTTACAAGTGAAAGATAGGGAGATAGCAACAATGACACGAACGGAAGCTAAAGCTACAGCAGCTTTTAAGGCCCAAATTGAGAAGCTACAGCAGGAGCGAGACGAGTTTCAGAGGATGGTCATTGGCAATCAGCAAGTTAGGACTCAACAAATGCATGAGATGAAGAAAAAAGAAAAGGAGTACATAAAGTTGCAGGAGAGGCTAAACCAAGTGTTGATGGAGAAAAAGAAGGAATCTAGATCAGGCATGGAGATAATGAATTTGCTTCAGAAAGAAGGGAGGCAGCGTGGAACATGGAATGGAAAGAAGGCTGATAATGATTTTCATAAAAAGATTGTGGATGCTTATGAGGCAAAAAATCAAGAACTAATGGCAGAGAATTCCGATTTAAAGGCATTATTACGATCCATGCAGGTTGATATGCGCGATTTCATAAATGCTCCAAATGGGCTCTCCAAGCAGTCCCGGGATGTAAATGAACGAGTTGAAACTGACCACACTCTGTCTCCACTGGGCGGGAGGACGGATGTCTTTGACTTGCCTTTTCACATGGCTAGAGATCAAATAGAAGAAAATCTTCGAAATAAGATGGCTTCCATAAAGGAGCGCATGGTTCAATTGCAAGATGCACAAAAAGAAGCAGAGGTTACATCTGAAGTAACCGAGAGGGAGCTTGAGCTTGAAGCTCAACTTGTTGAGGCGAGGAGCATAATCCAAGAGCAGGCATCCATAATGGCTAAACAATTGGCTAAGTCCGAGAGGCCAAGGAATCTGAATGGCAGCCATTTTAATTCTGGCGGGGAATCTAATATTTCATCACCTGCTGAGGGGGTAAGAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04520 map04520
Pfam Domains
Protein Families
ADIP

Protein Analysis

390

Amino Acids

44.6

Weight (kDa)

5.89

Isoelectric Point (pI)

47.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADIP PF11559 32 - 179 2.7e-47 Afadin- and alpha -actinin-Binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1156
Acc36I ACCTGC 2 cut(s) 730, 1156
AccII CGCG 1 cut(s) 753
AciI CCGC 5 cut(s) 48, 252, 271, 843, 1125
AclWI GGATC 2 cut(s) 164, 726
AcsI RAATTY 4 cut(s) 88, 239, 586, 706
AcuI CTGAAG 2 cut(s) 578, 1002
AcyI GRCGYC 1 cut(s) 146
AfaI GTAC 1 cut(s) 509
AfiI CCNNNNNNNGG 4 cut(s) 84, 842, 1048, 1154
AgsI TTSAA 5 cut(s) 115, 239, 815, 944, 1010
AluBI AGCT 8 cut(s) 302, 374, 380, 389, 412, 1000, 1006, 1013
AluI AGCT 8 cut(s) 302, 374, 380, 389, 412, 1000, 1006, 1013
Alw26I GTCTC 4 cut(s) 217, 259, 420, 837
AlwI GGATC 2 cut(s) 164, 726
AlwNI CAGNNNCTG 1 cut(s) 274
Ama87I CYCGRG 1 cut(s) 792
AoxI GGCC 2 cut(s) 396, 1088
ApeKI GCWGC 3 cut(s) 386, 610, 1107
ApoI RAATTY 4 cut(s) 88, 239, 586, 706
ArsI GACNNNNNNTTYG 1 cut(s) 37
Asp700I GAANNNNTTC 1 cut(s) 903
AspLEI GCGC 2 cut(s) 753, 936
AspS9I GGNCC 1 cut(s) 397
AsuC2I CCSGG 3 cut(s) 143, 793, 794
AsuHPI GGTGA 1 cut(s) 1137
AsuII TTCGAA 1 cut(s) 907
AvaI CYCGRG 1 cut(s) 792
BanII GRGCYC 1 cut(s) 780
BbvCI CCTCAGC 1 cut(s) 1152
BbvI GCAGC 3 cut(s) 398, 622, 1119
BccI CCATC 4 cut(s) 59, 436, 538, 910
BcnI CCSGG 3 cut(s) 143, 793, 794
BcoDI GTCTC 4 cut(s) 217, 259, 420, 837
BfaI CTAG 2 cut(s) 564, 882
BfmI CTRYAG 2 cut(s) 381, 413
BfuAI ACCTGC 2 cut(s) 730, 1156
BisI GCNGC 4 cut(s) 272, 387, 611, 1108
BlsI GCNGC 4 cut(s) 273, 388, 612, 1109
Bme1390I CCNGG 3 cut(s) 143, 793, 794
BmeT110I CYCGRG 1 cut(s) 792
BmgT120I GGNCC 1 cut(s) 397
BmrFI CCNGG 3 cut(s) 143, 793, 794
BmrI ACTGGG 1 cut(s) 848
BmsI GCATC 3 cut(s) 658, 943, 1061
BmuI ACTGGG 1 cut(s) 848
BoxI GACNNNNGTC 1 cut(s) 854
Bpu10I CCTNAGC 1 cut(s) 1152
Bpu14I TTCGAA 1 cut(s) 907
BpuEI CTTGAG 2 cut(s) 338, 1022
BpuMI CCSGG 3 cut(s) 143, 793, 794
BsaHI GRCGYC 1 cut(s) 146
BsaJI CCNNGG 2 cut(s) 792, 1091
BsaWI WCCGGW 1 cut(s) 172
BsaXI ACNNNNNCTCC 4 cut(s) 598, 628, 817, 847
Bsc4I CCNNNNNNNGG 4 cut(s) 84, 842, 1048, 1154
Bse1I ACTGG 1 cut(s) 843
Bse3DI GCAATG 1 cut(s) 97
BseDI CCNNGG 2 cut(s) 792, 1091
BseGI GGATG 7 cut(s) 51, 238, 447, 673, 802, 859, 1052
BseLI CCNNNNNNNGG 4 cut(s) 84, 842, 1048, 1154
BseMI GCAATG 1 cut(s) 97
BseMII CTCAG 3 cut(s) 43, 56, 1143
BseNI ACTGG 1 cut(s) 843
BseRI GAGGAG 1 cut(s) 1045
BseXI GCAGC 3 cut(s) 398, 622, 1119
Bsh1236I CGCG 1 cut(s) 753
BshFI GGCC 2 cut(s) 398, 1090
BsiHKCI CYCGRG 1 cut(s) 792
BsiSI CCGG 3 cut(s) 143, 173, 793
BslFI GGGAC 1 cut(s) 775
BslI CCNNNNNNNGG 4 cut(s) 84, 842, 1048, 1154
BsmAI GTCTC 4 cut(s) 217, 259, 420, 837
BsmBI CGTCTC 1 cut(s) 420
BsmFI GGGAC 1 cut(s) 775
BsnI GGCC 2 cut(s) 398, 1090
BsoBI CYCGRG 1 cut(s) 792
Bsp119I TTCGAA 1 cut(s) 907
Bsp1286I GDGCHC 1 cut(s) 780
Bsp143I GATC 6 cut(s) 25, 154, 169, 566, 731, 886
BspACI CCGC 5 cut(s) 48, 252, 271, 843, 1125
BspANI GGCC 2 cut(s) 398, 1090
BspCNI CTCAG 3 cut(s) 42, 55, 1144
BspFNI CGCG 1 cut(s) 753
BspMI ACCTGC 2 cut(s) 730, 1156
BspPI GGATC 2 cut(s) 164, 726
BspT104I TTCGAA 1 cut(s) 907
BsrDI GCAATG 1 cut(s) 97
BsrI ACTGG 1 cut(s) 843
BssECI CCNNGG 2 cut(s) 792, 1091
BssMI GATC 6 cut(s) 25, 154, 169, 566, 731, 886
BssNI GRCGYC 1 cut(s) 146
BssT1I CCWWGG 1 cut(s) 1091
Bst4CI ACNGT 1 cut(s) 36
Bst6I CTCTTC 1 cut(s) 164
BstACI GRCGYC 1 cut(s) 146
BstBI TTCGAA 1 cut(s) 907
BstC8I GCNNGC 2 cut(s) 6, 1050
BstDEI CTNAG 4 cut(s) 29, 42, 1077, 1152
BstF5I GGATG 7 cut(s) 51, 238, 447, 673, 802, 859, 1052
BstFNI CGCG 1 cut(s) 753
BstHHI GCGC 2 cut(s) 753, 936
BstKTI GATC 6 cut(s) 28, 157, 172, 569, 734, 889
BstMAI GTCTC 4 cut(s) 217, 259, 420, 837
BstMBI GATC 6 cut(s) 25, 154, 169, 566, 731, 886
BstMWI GCNNNNNNNGC 6 cut(s) 322, 386, 395, 526, 677, 784
BstPAI GACNNNNGTC 1 cut(s) 854
BstSCI CCNGG 3 cut(s) 141, 791, 792
BstSFI CTRYAG 2 cut(s) 381, 413
BstUI CGCG 1 cut(s) 753
BstV1I GCAGC 3 cut(s) 398, 622, 1119
BsuRI GGCC 2 cut(s) 398, 1090
BtsCI GGATG 7 cut(s) 51, 238, 447, 673, 802, 859, 1052
BtsIMutI CAGTG 1 cut(s) 836
BveI ACCTGC 2 cut(s) 730, 1156
Cac8I GCNNGC 2 cut(s) 6, 1050
CaiI CAGNNNCTG 1 cut(s) 274
CfoI GCGC 2 cut(s) 753, 936
Cfr13I GGNCC 1 cut(s) 397
Cfr9I CCCGGG 1 cut(s) 792
CseI GACGC 1 cut(s) 135
Csp6I GTAC 1 cut(s) 508
CviAII CATG 6 cut(s) 482, 574, 621, 736, 877, 937
CviQI GTAC 1 cut(s) 508
DdeI CTNAG 4 cut(s) 29, 42, 1077, 1152
DpnI GATC 6 cut(s) 27, 156, 171, 568, 733, 888
DpnII GATC 6 cut(s) 25, 154, 169, 566, 731, 886
DraI TTTAAA 1 cut(s) 717
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
EciI GGCGGA 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 1091
Eco24I GRGCYC 1 cut(s) 780
Eco57I CTGAAG 2 cut(s) 578, 1002
Eco88I CYCGRG 1 cut(s) 792
EcoRI GAATTC 1 cut(s) 706
EcoT14I CCWWGG 1 cut(s) 1091
EcoT22I ATGCAT 1 cut(s) 483
EcoT38I GRGCYC 1 cut(s) 780
ErhI CCWWGG 1 cut(s) 1091
Esp3I CGTCTC 1 cut(s) 420
FaeI CATG 6 cut(s) 485, 577, 624, 739, 880, 940
FalI AAGNNNNNCTT 4 cut(s) 888, 920, 1002, 1034
FaqI GGGAC 1 cut(s) 775
FatI CATG 6 cut(s) 481, 573, 620, 735, 876, 936
FauI CCCGC 2 cut(s) 836, 1118
Fnu4HI GCNGC 4 cut(s) 272, 387, 611, 1108
FokI GGATG 7 cut(s) 38, 245, 454, 680, 809, 866, 1039
FriOI GRGCYC 1 cut(s) 780
Fsp4HI GCNGC 4 cut(s) 272, 387, 611, 1108
FspBI CTAG 2 cut(s) 564, 882
GlaI GCGC 2 cut(s) 752, 935
GluI GCNGC 4 cut(s) 272, 387, 611, 1108
HaeIII GGCC 2 cut(s) 398, 1090
HapII CCGG 3 cut(s) 143, 173, 793
HgaI GACGC 1 cut(s) 135
HhaI GCGC 2 cut(s) 753, 936
Hin1I GRCGYC 1 cut(s) 146
Hin1II CATG 6 cut(s) 485, 577, 624, 739, 880, 940
Hin6I GCGC 2 cut(s) 751, 934
HinP1I GCGC 2 cut(s) 751, 934
HinfI GANTC 7 cut(s) 115, 138, 248, 469, 560, 1096, 1130
HpaII CCGG 3 cut(s) 143, 173, 793
HphI GGTGA 1 cut(s) 1137
Hpy188III TCNNGA 4 cut(s) 245, 291, 564, 689
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 4 cut(s) 142, 550, 596, 628
HpyCH4III ACNGT 1 cut(s) 36
HpyCH4V TGCA 7 cut(s) 102, 195, 481, 520, 739, 949, 956
HpyF10VI GCNNNNNNNGC 6 cut(s) 322, 386, 395, 526, 677, 784
HpyF3I CTNAG 4 cut(s) 29, 42, 1077, 1152
Hsp92I GRCGYC 1 cut(s) 146
Hsp92II CATG 6 cut(s) 485, 577, 624, 739, 880, 940
HspAI GCGC 2 cut(s) 751, 934
Kzo9I GATC 6 cut(s) 25, 154, 169, 566, 731, 886
LmnI GCTCC 6 cut(s) 94, 421, 772, 931, 997, 1032
Lsp1109I GCAGC 3 cut(s) 398, 622, 1119
LweI GCATC 3 cut(s) 658, 943, 1061
MaeI CTAG 2 cut(s) 564, 882
MaeIII GTNAC 5 cut(s) 127, 147, 327, 973, 985
MalI GATC 6 cut(s) 27, 156, 171, 568, 733, 888
MboI GATC 6 cut(s) 25, 154, 169, 566, 731, 886
MboII GAAGA 4 cut(s) 151, 502, 896, 908
MfeI CAATTG 3 cut(s) 196, 944, 1070
MhlI GDGCHC 1 cut(s) 780
MluCI AATT 9 cut(s) 88, 196, 239, 402, 586, 706, 944, 1070, 1117
MlyI GAGTC 2 cut(s) 257, 463
Mph1103I ATGCAT 1 cut(s) 483
MroXI GAANNNNTTC 1 cut(s) 903
MseI TTAA 4 cut(s) 105, 393, 716, 1116
MspA1I CMGCKG 1 cut(s) 271
MspI CCGG 3 cut(s) 143, 173, 793
MspR9I CCNGG 3 cut(s) 143, 793, 794
MunI CAATTG 3 cut(s) 196, 944, 1070
MvnI CGCG 1 cut(s) 753
MwoI GCNNNNNNNGC 6 cut(s) 322, 386, 395, 526, 677, 784
NciI CCSGG 3 cut(s) 143, 793, 794
NdeII GATC 6 cut(s) 25, 154, 169, 566, 731, 886
NlaIII CATG 6 cut(s) 485, 577, 624, 739, 880, 940
NmuCI GTSAC 1 cut(s) 147
NsiI ATGCAT 1 cut(s) 483
NspV TTCGAA 1 cut(s) 907
PaqCI CACCTGC 1 cut(s) 1156
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PdmI GAANNNNTTC 1 cut(s) 903
PfeI GAWTC 5 cut(s) 115, 138, 560, 1096, 1130
PkrI GCNGC 4 cut(s) 273, 388, 612, 1109
PleI GAGTC 2 cut(s) 256, 463
PpsI GAGTC 2 cut(s) 256, 463
PshAI GACNNNNGTC 1 cut(s) 854
PspPI GGNCC 1 cut(s) 397
PstNI CAGNNNCTG 1 cut(s) 274
RsaI GTAC 1 cut(s) 509
RsaNI GTAC 1 cut(s) 508
SaqAI TTAA 4 cut(s) 105, 393, 716, 1116
SatI GCNGC 4 cut(s) 272, 387, 611, 1108
Sau3AI GATC 6 cut(s) 25, 154, 169, 566, 731, 886
Sau96I GGNCC 1 cut(s) 397
SchI GAGTC 2 cut(s) 257, 463
ScrFI CCNGG 3 cut(s) 143, 793, 794
SduI GDGCHC 1 cut(s) 780
SfaNI GCATC 3 cut(s) 658, 943, 1061
SfcI CTRYAG 2 cut(s) 381, 413
SfuI TTCGAA 1 cut(s) 907
SmaI CCCGGG 1 cut(s) 794
SmlI CTYRAG 2 cut(s) 317, 1001
SmoI CTYRAG 2 cut(s) 317, 1001
Sse9I AATT 9 cut(s) 88, 196, 239, 402, 586, 706, 944, 1070, 1117
SsiI CCGC 5 cut(s) 48, 252, 271, 843, 1125
SspI AATATT 1 cut(s) 1138
SspMI CTAG 2 cut(s) 564, 882
StyD4I CCNGG 3 cut(s) 141, 791, 792
StyI CCWWGG 1 cut(s) 1091
TaaI ACNGT 1 cut(s) 36
TaqI TCGA 3 cut(s) 24, 244, 907
TasI AATT 9 cut(s) 88, 196, 239, 402, 586, 706, 944, 1070, 1117
TatI WGTACW 1 cut(s) 507
TauI GCSGC 1 cut(s) 274
TfiI GAWTC 5 cut(s) 115, 138, 560, 1096, 1130
Tru1I TTAA 4 cut(s) 105, 393, 716, 1116
Tru9I TTAA 4 cut(s) 105, 393, 716, 1116
TscAI CASTG 1 cut(s) 843
TseFI GTSAC 1 cut(s) 147
TseI GCWGC 3 cut(s) 386, 610, 1107
Tsp45I GTSAC 1 cut(s) 147
TspDTI ATGAA 7 cut(s) 273, 503, 599, 641, 748, 819, 1131
TspGWI ACGGA 3 cut(s) 26, 383, 866
TspMI CCCGGG 1 cut(s) 792
TspRI CASTG 1 cut(s) 843
XapI RAATTY 4 cut(s) 88, 239, 586, 706
XbaI TCTAGA 1 cut(s) 563
XcmI CCANNNNNNNNNTGG 1 cut(s) 1118
XmaI CCCGGG 1 cut(s) 792
XmnI GAANNNNTTC 1 cut(s) 903
XspI CTAG 2 cut(s) 564, 882
Zsp2I ATGCAT 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.