AT2G21170

triosephosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0009314
Physical Location & Seq
Reverse (-)
9070734 .. 9074470
3737 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G21170.2

Sequence Viewer

Length: 948 bp
ATGGCAGCTACCTCTCTCACTGCCCCTCCTTCTTTCTCCGGTCTCCGCCGCATTTCTCCCAAGCTCGACGCTGCCGCCGTCTCCTCCCACCAATCCTTCTTCCACCGCGTCAATTCCTCTACCCGTCTCGTTTCTTCCTCTTCTTCTTCTCATCGCTCCCCCAGAGGTGTTGTTGCCATGGCTGGATCCGGAAAGTTTTTCGTTGGAGGAAACTGGAAGTGTAACGGGACTAAGGACTCCATCGCCAAGCTTATCTCCGATCTCAACAGTGCAACCTTGGAAGCAGATGTAGATGTTGTTGTGTCACCTCCATTTGTCTACATCGACCAGGTCAAATCCTCGTTGACAGACCGTATTGACATATCAGGTCAGAACTCTTGGGTTGGGAAAGGTGGAGCCTTCACTGGTGAAATCAGCGTGGAACAGCTCAAAGACCTTGGCTGCAAGTGGGTCATTCTTGGGCATTCCGAACGGAGACATGTCATCGGAGAAAAAGATGAGTTTATCGGGAAGAAAGCTGCATATGCATTGAGTGAGGGTCTTGGAGTGATAGCTTGTATTGGGGAAAAGCTAGAAGAGAGGGAAGCAGGCAAGACGTTTGATGTTTGCTTCGCGCAACTGAAGGCGTTTGCTGATGCTGTGCCTAGCTGGGACAATATAGTTGTTGCATACGAGCCTGTATGGGCAATTGGAACTGGTAAAGTTGCATCTCCTCAGCAAGCACAAGAAGTCCATGTAGCTGTCCGCGGTTGGCTAAAGAAGAATGTCTCTGAGGAAGTTGCTTCCAAAACGAGAATCATATATGGAGGTTCTGTCAATGGAGGCAACAGTGCAGAGCTTGCCAAAGAAGAAGACATTGATGGATTTCTTGTTGGTGGTGCCTCCTTGAAGGGTCCTGAGTTTGCAACCATTGTGAACTCAGTCACGTCGAAGAAAGTTGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003674 GO:0003824 GO:0004807 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005975 GO:0005996 GO:0006006 GO:0006066 GO:0006071 GO:0006081 GO:0006082 GO:0006090 GO:0006091 GO:0006094 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006642 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009657 GO:0009658 GO:0009941 GO:0009987 GO:0016043 GO:0016051 GO:0016052 GO:0016053 GO:0016310 GO:0016853 GO:0016860 GO:0016861 GO:0017144 GO:0018130 GO:0019318 GO:0019319 GO:0019359 GO:0019362 GO:0019363 GO:0019400 GO:0019405 GO:0019438 GO:0019439 GO:0019563 GO:0019637 GO:0019682 GO:0019693 GO:0019751 GO:0019752 GO:0022622 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044262 GO:0044270 GO:0044271 GO:0044275 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046164 GO:0046166 GO:0046174 GO:0046184 GO:0046364 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046486 GO:0046496 GO:0046700 GO:0046939 GO:0048046 GO:0048364 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0071840 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0080022 GO:0090407 GO:0099402 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901616
KEGG Pathways
Metabolic & Signaling
ko00010 ko00051 ko00562 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00051 map00562 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
TIM

Protein Analysis

315

Amino Acids

33.35

Weight (kDa)

7.67

Isoelectric Point (pI)

37.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIM PF00121 66 - 298 7.9e-86 Triosephosphate isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 878
AccI GTMKAC 1 cut(s) 318
AccII CGCG 3 cut(s) 108, 614, 747
AccIII TCCGGA 1 cut(s) 188
AciI CCGC 6 cut(s) 46, 49, 75, 106, 745, 747
AclWI GGATC 2 cut(s) 180, 193
AcuI CTGAAG 1 cut(s) 641
AflIII ACRYGT 1 cut(s) 478
AgsI TTSAA 1 cut(s) 889
AjiI CACGTC 1 cut(s) 927
AjnI CCWGG 1 cut(s) 327
Alw26I GTCTC 5 cut(s) 47, 85, 131, 469, 772
AlwI GGATC 2 cut(s) 180, 193
Aor13HI TCCGGA 1 cut(s) 188
ApeKI GCWGC 5 cut(s) 5, 71, 441, 518, 941
AspLEI GCGC 1 cut(s) 616
AspS9I GGNCC 1 cut(s) 893
AsuHPI GGTGA 2 cut(s) 297, 419
AvaII GGWCC 1 cut(s) 893
BamHI GGATCC 1 cut(s) 185
BanI GGYRCC 1 cut(s) 878
BbsI GAAGAC 1 cut(s) 858
BbvCI CCTCAGC 1 cut(s) 714
BbvI GCAGC 5 cut(s) 17, 58, 428, 505, 928
BccI CCATC 2 cut(s) 248, 854
BceAI ACGGC 1 cut(s) 62
BciT130I CCWGG 1 cut(s) 329
BcoDI GTCTC 5 cut(s) 47, 85, 131, 469, 772
BfaI CTAG 2 cut(s) 572, 645
BisI GCNGC 7 cut(s) 6, 49, 72, 75, 442, 519, 942
BlsI GCNGC 7 cut(s) 7, 50, 73, 76, 443, 520, 943
Bme1390I CCNGG 1 cut(s) 329
Bme18I GGWCC 1 cut(s) 893
BmgBI CACGTC 1 cut(s) 927
BmgT120I GGNCC 1 cut(s) 893
BmiI GGNNCC 4 cut(s) 187, 397, 880, 894
BmrFI CCNGG 1 cut(s) 329
BmsI GCATC 2 cut(s) 625, 716
BpiI GAAGAC 1 cut(s) 858
Bpu10I CCTNAGC 1 cut(s) 714
BsaI GGTCTC 1 cut(s) 47
BsaJI CCNNGG 4 cut(s) 177, 276, 436, 745
BsaWI WCCGGW 2 cut(s) 38, 188
BsaXI ACNNNNNCTCC 4 cut(s) 10, 40, 798, 828
Bse1I ACTGG 3 cut(s) 218, 409, 700
BseAI TCCGGA 1 cut(s) 188
BseBI CCWGG 1 cut(s) 329
BseDI CCNNGG 4 cut(s) 177, 276, 436, 745
BseMII CTCAG 4 cut(s) 728, 762, 888, 933
BseNI ACTGG 3 cut(s) 218, 409, 700
BseRI GAGGAG 2 cut(s) 73, 702
BseXI GCAGC 5 cut(s) 17, 58, 428, 505, 928
BseYI CCCAGC 1 cut(s) 648
BsgI GTGCAG 1 cut(s) 852
Bsh1236I CGCG 3 cut(s) 108, 614, 747
BshNI GGYRCC 1 cut(s) 878
BsiSI CCGG 2 cut(s) 39, 189
BslFI GGGAC 2 cut(s) 241, 665
BsmAI GTCTC 5 cut(s) 47, 85, 131, 469, 772
BsmBI CGTCTC 2 cut(s) 85, 131
BsmFI GGGAC 2 cut(s) 241, 665
BsmI GAATGC 1 cut(s) 463
Bso31I GGTCTC 1 cut(s) 47
Bsp13I TCCGGA 1 cut(s) 188
Bsp143I GATC 2 cut(s) 185, 259
Bsp19I CCATGG 1 cut(s) 177
BspACI CCGC 6 cut(s) 46, 49, 75, 106, 745, 747
BspCNI CTCAG 4 cut(s) 727, 763, 889, 932
BspEI TCCGGA 1 cut(s) 188
BspFNI CGCG 3 cut(s) 108, 614, 747
BspLI GGNNCC 4 cut(s) 187, 397, 880, 894
BspPI GGATC 2 cut(s) 180, 193
BspT107I GGYRCC 1 cut(s) 878
BspTNI GGTCTC 1 cut(s) 47
BsrI ACTGG 3 cut(s) 218, 409, 700
BssECI CCNNGG 4 cut(s) 177, 276, 436, 745
BssMI GATC 2 cut(s) 185, 259
BssT1I CCWWGG 3 cut(s) 177, 276, 436
Bst2UI CCWGG 1 cut(s) 329
Bst4CI ACNGT 3 cut(s) 269, 353, 830
Bst6I CTCTTC 2 cut(s) 145, 570
BstAPI GCANNNNNTGC 1 cut(s) 839
BstC8I GCNNGC 3 cut(s) 589, 720, 840
BstDEI CTNAG 5 cut(s) 231, 714, 771, 897, 919
BstDSI CCRYGG 2 cut(s) 177, 745
BstFNI CGCG 3 cut(s) 108, 614, 747
BstHHI GCGC 1 cut(s) 616
BstKTI GATC 2 cut(s) 188, 262
BstMAI GTCTC 5 cut(s) 47, 85, 131, 469, 772
BstMBI GATC 2 cut(s) 185, 259
BstMWI GCNNNNNNNGC 2 cut(s) 524, 839
BstNI CCWGG 1 cut(s) 329
BstNSI RCATGY 1 cut(s) 482
BstSCI CCNGG 1 cut(s) 327
BstUI CGCG 3 cut(s) 108, 614, 747
BstV1I GCAGC 5 cut(s) 17, 58, 428, 505, 928
BstV2I GAAGAC 1 cut(s) 858
BstX2I RGATCY 1 cut(s) 185
BstYI RGATCY 1 cut(s) 185
BtgI CCRYGG 2 cut(s) 177, 745
BtgZI GCGATG 2 cut(s) 137, 226
BtrI CACGTC 1 cut(s) 927
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 4 cut(s) 18, 274, 402, 835
Cac8I GCNNGC 3 cut(s) 589, 720, 840
CfoI GCGC 1 cut(s) 616
Cfr13I GGNCC 1 cut(s) 893
Cfr42I CCGCGG 1 cut(s) 748
CseI GACGC 2 cut(s) 77, 97
CsiI ACCWGGT 1 cut(s) 327
CviAII CATG 3 cut(s) 178, 479, 734
DdeI CTNAG 5 cut(s) 231, 714, 771, 897, 919
DpnI GATC 2 cut(s) 187, 261
DpnII GATC 2 cut(s) 185, 259
Eam1104I CTCTTC 2 cut(s) 145, 570
EarI CTCTTC 2 cut(s) 145, 570
EciI GGCGGA 1 cut(s) 35
Eco130I CCWWGG 3 cut(s) 177, 276, 436
Eco31I GGTCTC 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 893
Eco57I CTGAAG 1 cut(s) 641
EcoO109I RGGNCCY 1 cut(s) 893
EcoRII CCWGG 1 cut(s) 327
EcoT14I CCWWGG 3 cut(s) 177, 276, 436
EcoT22I ATGCAT 1 cut(s) 529
ErhI CCWWGG 3 cut(s) 177, 276, 436
Esp3I CGTCTC 2 cut(s) 85, 131
FaeI CATG 3 cut(s) 181, 482, 737
FaqI GGGAC 2 cut(s) 241, 665
FatI CATG 3 cut(s) 177, 478, 733
FauNDI CATATG 1 cut(s) 523
FblI GTMKAC 1 cut(s) 318
Fnu4HI GCNGC 7 cut(s) 6, 49, 72, 75, 442, 519, 942
Fsp4HI GCNGC 7 cut(s) 6, 49, 72, 75, 442, 519, 942
FspBI CTAG 2 cut(s) 572, 645
GlaI GCGC 1 cut(s) 615
GluI GCNGC 7 cut(s) 6, 49, 72, 75, 442, 519, 942
GsaI CCCAGC 1 cut(s) 652
HapII CCGG 2 cut(s) 39, 189
HgaI GACGC 2 cut(s) 77, 97
HhaI GCGC 1 cut(s) 616
Hin1II CATG 3 cut(s) 181, 482, 737
Hin6I GCGC 1 cut(s) 614
HinP1I GCGC 1 cut(s) 614
HincII GTYRAC 1 cut(s) 345
HindII GTYRAC 1 cut(s) 345
HindIII AAGCTT 1 cut(s) 248
HinfI GANTC 2 cut(s) 236, 795
HpaII CCGG 2 cut(s) 39, 189
HphI GGTGA 2 cut(s) 297, 419
Hpy166II GTNNAC 3 cut(s) 319, 345, 916
Hpy188I TCNGA 5 cut(s) 259, 372, 469, 488, 772
Hpy188III TCNNGA 3 cut(s) 189, 508, 896
Hpy8I GTNNAC 3 cut(s) 319, 345, 916
Hpy99I CGWCG 2 cut(s) 71, 931
HpyAV CCTTC 5 cut(s) 39, 106, 409, 616, 883
HpyCH4III ACNGT 3 cut(s) 269, 353, 830
HpyCH4IV ACGT 2 cut(s) 596, 926
HpyCH4V TGCA 8 cut(s) 272, 444, 521, 527, 668, 707, 833, 905
HpyF10VI GCNNNNNNNGC 2 cut(s) 524, 839
HpyF3I CTNAG 5 cut(s) 231, 714, 771, 897, 919
HpySE526I ACGT 2 cut(s) 596, 926
Hsp92II CATG 3 cut(s) 181, 482, 737
HspAI GCGC 1 cut(s) 614
Kpn2I TCCGGA 1 cut(s) 188
KspI CCGCGG 1 cut(s) 748
Kzo9I GATC 2 cut(s) 185, 259
LmnI GCTCC 2 cut(s) 161, 395
Lsp1109I GCAGC 5 cut(s) 17, 58, 428, 505, 928
LweI GCATC 2 cut(s) 625, 716
MabI ACCWGGT 1 cut(s) 327
MaeI CTAG 2 cut(s) 572, 645
MaeII ACGT 2 cut(s) 596, 926
MaeIII GTNAC 3 cut(s) 221, 303, 922
MalI GATC 2 cut(s) 187, 261
MboI GATC 2 cut(s) 185, 259
MfeI CAATTG 1 cut(s) 687
MflI RGATCY 1 cut(s) 185
MluCI AATT 2 cut(s) 112, 687
MlyI GAGTC 1 cut(s) 230
MmeI TCCRAC 1 cut(s) 184
Mph1103I ATGCAT 1 cut(s) 529
MroI TCCGGA 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 747
MspI CCGG 2 cut(s) 39, 189
MspR9I CCNGG 1 cut(s) 329
MunI CAATTG 1 cut(s) 687
Mva1269I GAATGC 1 cut(s) 463
MvaI CCWGG 1 cut(s) 329
MvnI CGCG 3 cut(s) 108, 614, 747
MwoI GCNNNNNNNGC 2 cut(s) 524, 839
NcoI CCATGG 1 cut(s) 177
NdeI CATATG 1 cut(s) 523
NdeII GATC 2 cut(s) 185, 259
NlaIII CATG 3 cut(s) 181, 482, 737
NlaIV GGNNCC 4 cut(s) 187, 397, 880, 894
NmuCI GTSAC 2 cut(s) 303, 922
NsiI ATGCAT 1 cut(s) 529
NspI RCATGY 1 cut(s) 482
PciI ACATGT 1 cut(s) 478
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 463
PfeI GAWTC 1 cut(s) 795
PflFI GACNNNGTC 1 cut(s) 329
PkrI GCNGC 7 cut(s) 7, 50, 73, 76, 443, 520, 943
PleI GAGTC 1 cut(s) 230
PpsI GAGTC 1 cut(s) 230
PpuMI RGGWCCY 1 cut(s) 893
PscI ACATGT 1 cut(s) 478
Psp5II RGGWCCY 1 cut(s) 893
Psp6I CCWGG 1 cut(s) 327
PspFI CCCAGC 1 cut(s) 648
PspGI CCWGG 1 cut(s) 327
PspN4I GGNNCC 4 cut(s) 187, 397, 880, 894
PspPI GGNCC 1 cut(s) 893
PspPPI RGGWCCY 1 cut(s) 893
PsuI RGATCY 1 cut(s) 185
PsyI GACNNNGTC 1 cut(s) 329
SacII CCGCGG 1 cut(s) 748
SatI GCNGC 7 cut(s) 6, 49, 72, 75, 442, 519, 942
Sau3AI GATC 2 cut(s) 185, 259
Sau96I GGNCC 1 cut(s) 893
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 1 cut(s) 329
SexAI ACCWGGT 1 cut(s) 327
SfaNI GCATC 2 cut(s) 625, 716
Sfr303I CCGCGG 1 cut(s) 748
SgrBI CCGCGG 1 cut(s) 748
SinI GGWCC 1 cut(s) 893
Sse9I AATT 2 cut(s) 112, 687
SsiI CCGC 6 cut(s) 46, 49, 75, 106, 745, 747
SspMI CTAG 2 cut(s) 572, 645
StyD4I CCNGG 1 cut(s) 327
StyI CCWWGG 3 cut(s) 177, 276, 436
TaaI ACNGT 3 cut(s) 269, 353, 830
TaiI ACGT 2 cut(s) 599, 929
TaqI TCGA 3 cut(s) 66, 324, 929
TasI AATT 2 cut(s) 112, 687
TauI GCSGC 2 cut(s) 51, 77
TfiI GAWTC 1 cut(s) 795
TscAI CASTG 4 cut(s) 25, 274, 409, 835
TseFI GTSAC 2 cut(s) 303, 922
TseI GCWGC 5 cut(s) 5, 71, 441, 518, 941
Tsp45I GTSAC 2 cut(s) 303, 922
TspGWI ACGGA 1 cut(s) 487
TspRI CASTG 4 cut(s) 25, 274, 409, 835
Tth111I GACNNNGTC 1 cut(s) 329
VpaK11BI GGWCC 1 cut(s) 893
XceI RCATGY 1 cut(s) 482
XmiI GTMKAC 1 cut(s) 318
XspI CTAG 2 cut(s) 572, 645
Zsp2I ATGCAT 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.