Prupe.8G160500_v2.0.a1

Triosephosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0009314
Physical Location & Seq
Reverse (-)
16906114 .. 16910351
4238 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G160500.2

Sequence Viewer

Length: 1065 bp
ATGGTGGACTGTTCCTATATAGTGACTCACTCTCTCACTCGTGAAATACAAGTAAAAACACAGAGGAAGCAAACCCAAACTCGAAAGCTCCAACTCACTGAGTTAGGCATGTCGGTGGCCTCCACATCTCTCGCTTCCCAACTCTCTGGCCCTAAATCCGTCTCCTCTTACTCCGGTCTGCGACGATCGTGCTCCAAGTTGGACCACACCCAGTCTCTCTCCCTCTTCCAACATCTTCACTCCCAGCTCCGCCTCTCTTCCTCCTCCCGCAAAGCCTCCAGAGGCATCGTCGCCATGGCCGGCACTGGAAAGTTCTTTGTTGGTGGAAACTGGAAGTGTAATGGCACAAAAGACTCTATCAGCAAGCTAGTCTCTGACTTGAACAGTGCCAAATTGGAAGCAGATGTTGATGTTATTGTTGCACCACCATTTCTTTACATCGATCAGGTGAAGAACTCATTAACAGATCGTATTGAAATATCTGGTCAAAATTCTTGGGTTGGAAAAGGTGGCGCTTTCACGGGAGAAATCAGCGTTGAACAATTGAAGGATATTGGGGCCACATGGGTTATTCTTGGACACTCGGAACGGAGGCATGTAATTGGGGAAGACGATCAGTTTATAGGAAAGAAAGCTGCCTATGCCTTGAACGAGGGTCTTGGAGTTATTGCTTGCATTGGTGAGAAGCTAGAAGAAAGGGAAGCAGGGAAAACTTTTGACATCTGCTTTCAGCAACTGAAGGCTTTTGCAGATGCAGTACCCAGCTGGGATAATATAGTTATTGCTTATGAGCCTGTATGGGCCATTGGAACTGGTAAGGTGGCCAGTCCAGAACAAGCTCAGGAAGTACATGTAGCTGTTCGTGATTGGCTCAAAAAGAATGTGTCACCAGAAGTTGCATCCAAAACAAGAATTATTTATGGAGGGTCTGTAAATGGAGGCAATTCTGCTGAGCTTGCAAAGAAGGAAGATATTGATGGTTTTCTTGTTGGTGGTGCTTCCTTAAAGGGTCCTGAATTCGCTACCATCATCAATTCCGTAACAGCCAAGAAAGTTGCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003674 GO:0003824 GO:0004807 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005975 GO:0005996 GO:0006006 GO:0006066 GO:0006071 GO:0006081 GO:0006082 GO:0006090 GO:0006091 GO:0006094 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006642 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009657 GO:0009658 GO:0009941 GO:0009987 GO:0016043 GO:0016051 GO:0016052 GO:0016053 GO:0016310 GO:0016853 GO:0016860 GO:0016861 GO:0017144 GO:0018130 GO:0019318 GO:0019319 GO:0019359 GO:0019362 GO:0019363 GO:0019400 GO:0019405 GO:0019438 GO:0019439 GO:0019563 GO:0019637 GO:0019682 GO:0019693 GO:0019751 GO:0019752 GO:0022622 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044262 GO:0044270 GO:0044271 GO:0044275 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046164 GO:0046166 GO:0046174 GO:0046184 GO:0046364 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046486 GO:0046496 GO:0046700 GO:0046939 GO:0048046 GO:0048364 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0071840 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0080022 GO:0090407 GO:0099402 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901616
KEGG Pathways
Metabolic & Signaling
ko00010 ko00051 ko00562 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00051 map00562 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
TIM

Protein Analysis

355

Amino Acids

38.14

Weight (kDa)

8.81

Isoelectric Point (pI)

32.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 250, 268
AcoI YGGCCR 2 cut(s) 297, 822
AcsI RAATTY 2 cut(s) 490, 1016
AcuI CTGAAG 1 cut(s) 758
AfaI GTAC 2 cut(s) 759, 849
AflIII ACRYGT 1 cut(s) 850
AgsI TTSAA 5 cut(s) 382, 476, 539, 547, 649
Alw21I GWGCWC 1 cut(s) 194
Alw26I GTCTC 3 cut(s) 166, 219, 376
AlwNI CAGNNNCTG 1 cut(s) 736
AoxI GGCC 6 cut(s) 117, 148, 297, 558, 801, 822
ApeKI GCWGC 2 cut(s) 635, 1058
ApoI RAATTY 2 cut(s) 490, 1016
AspLEI GCGC 1 cut(s) 515
AspS9I GGNCC 5 cut(s) 149, 202, 558, 801, 1010
AsuHPI GGTGA 3 cut(s) 460, 692, 879
AvaII GGWCC 2 cut(s) 202, 1010
BalI TGGCCA 1 cut(s) 824
BauI CACGAG 1 cut(s) 39
BbsI GAAGAC 1 cut(s) 615
Bbv12I GWGCWC 1 cut(s) 194
BbvI GCAGC 1 cut(s) 622
BccI CCATC 2 cut(s) 971, 1034
BcgI CGANNNNNNTGC 2 cut(s) 171, 205
BcoDI GTCTC 3 cut(s) 166, 219, 376
BfaI CTAG 2 cut(s) 368, 689
BfoI RGCGCY 1 cut(s) 516
BisI GCNGC 2 cut(s) 636, 1059
BlpI GCTNAGC 1 cut(s) 951
BlsI GCNGC 2 cut(s) 637, 1060
Bme18I GGWCC 2 cut(s) 202, 1010
BmgT120I GGNCC 5 cut(s) 149, 202, 558, 801, 1010
BmiI GGNNCC 2 cut(s) 559, 1011
BmrI ACTGGG 1 cut(s) 205
BmsI GCATC 3 cut(s) 294, 742, 908
BmuI ACTGGG 1 cut(s) 205
BpiI GAAGAC 1 cut(s) 615
BpmI CTGGAG 1 cut(s) 262
Bpu10I CCTNAGC 1 cut(s) 840
Bpu1102I GCTNAGC 1 cut(s) 951
Bsa29I ATCGAT 1 cut(s) 441
BsaJI CCNNGG 1 cut(s) 294
BsaWI WCCGGW 1 cut(s) 173
BsaXI ACNNNNNCTCC 2 cut(s) 915, 945
Bse118I RCCGGY 1 cut(s) 299
Bse1I ACTGG 5 cut(s) 211, 310, 335, 817, 825
BseCI ATCGAT 1 cut(s) 441
BseDI CCNNGG 1 cut(s) 294
BseGI GGATG 1 cut(s) 899
BseMII CTCAG 3 cut(s) 90, 854, 942
BseNI ACTGG 5 cut(s) 211, 310, 335, 817, 825
BseRI GAGGAG 2 cut(s) 154, 253
BseXI GCAGC 1 cut(s) 622
BseYI CCCAGC 3 cut(s) 243, 761, 765
Bsh1285I CGRYCG 1 cut(s) 188
BshFI GGCC 6 cut(s) 119, 150, 299, 560, 803, 824
BshVI ATCGAT 1 cut(s) 441
BsiEI CGRYCG 1 cut(s) 188
BsiHKAI GWGCWC 1 cut(s) 194
BsiSI CCGG 2 cut(s) 174, 300
BsmAI GTCTC 3 cut(s) 166, 219, 376
BsmBI CGTCTC 1 cut(s) 166
BsnI GGCC 6 cut(s) 119, 150, 299, 560, 803, 824
Bsp1286I GDGCHC 1 cut(s) 194
Bsp143I GATC 4 cut(s) 185, 442, 466, 613
Bsp1720I GCTNAGC 1 cut(s) 951
Bsp19I CCATGG 1 cut(s) 294
BspACI CCGC 2 cut(s) 250, 268
BspANI GGCC 6 cut(s) 119, 150, 299, 560, 803, 824
BspCNI CTCAG 3 cut(s) 91, 853, 943
BspDI ATCGAT 1 cut(s) 441
BspLI GGNNCC 2 cut(s) 559, 1011
BsrFI RCCGGY 1 cut(s) 299
BsrI ACTGG 5 cut(s) 211, 310, 335, 817, 825
BssAI RCCGGY 1 cut(s) 299
BssECI CCNNGG 1 cut(s) 294
BssMI GATC 4 cut(s) 185, 442, 466, 613
BssSI CACGAG 1 cut(s) 39
BssT1I CCWWGG 1 cut(s) 294
Bst2BI CACGAG 1 cut(s) 39
Bst4CI ACNGT 2 cut(s) 11, 386
Bst6I CTCTTC 2 cut(s) 230, 262
BstC8I GCNNGC 4 cut(s) 301, 365, 673, 957
BstDEI CTNAG 3 cut(s) 99, 840, 951
BstDSI CCRYGG 1 cut(s) 294
BstF5I GGATG 1 cut(s) 899
BstH2I RGCGCY 1 cut(s) 516
BstHHI GCGC 1 cut(s) 515
BstKTI GATC 4 cut(s) 188, 445, 469, 616
BstMAI GTCTC 3 cut(s) 166, 219, 376
BstMBI GATC 4 cut(s) 185, 442, 466, 613
BstMCI CGRYCG 1 cut(s) 188
BstMWI GCNNNNNNNGC 2 cut(s) 641, 956
BstNSI RCATGY 3 cut(s) 112, 599, 854
BstV1I GCAGC 1 cut(s) 622
BstV2I GAAGAC 1 cut(s) 615
BstXI CCANNNNNNTGG 1 cut(s) 146
Bsu15I ATCGAT 1 cut(s) 441
BsuRI GGCC 6 cut(s) 119, 150, 299, 560, 803, 824
BsuTUI ATCGAT 1 cut(s) 441
BtgI CCRYGG 1 cut(s) 294
BtsCI GGATG 1 cut(s) 899
BtsIMutI CAGTG 3 cut(s) 96, 303, 391
Cac8I GCNNGC 4 cut(s) 301, 365, 673, 957
CaiI CAGNNNCTG 1 cut(s) 736
CfoI GCGC 1 cut(s) 515
Cfr10I RCCGGY 1 cut(s) 299
Cfr13I GGNCC 5 cut(s) 149, 202, 558, 801, 1010
ClaI ATCGAT 1 cut(s) 441
Csp6I GTAC 2 cut(s) 758, 848
CviAII CATG 5 cut(s) 109, 295, 564, 596, 851
CviQI GTAC 2 cut(s) 758, 848
DdeI CTNAG 3 cut(s) 99, 840, 951
DpnI GATC 4 cut(s) 187, 444, 468, 615
DpnII GATC 4 cut(s) 185, 442, 466, 613
EaeI YGGCCR 2 cut(s) 297, 822
Eam1104I CTCTTC 2 cut(s) 230, 262
EarI CTCTTC 2 cut(s) 230, 262
EciI GGCGGA 1 cut(s) 239
Eco130I CCWWGG 1 cut(s) 294
Eco47I GGWCC 2 cut(s) 202, 1010
Eco57I CTGAAG 1 cut(s) 758
EcoO109I RGGNCCY 1 cut(s) 1010
EcoRI GAATTC 1 cut(s) 1016
EcoT14I CCWWGG 1 cut(s) 294
ErhI CCWWGG 1 cut(s) 294
Esp3I CGTCTC 1 cut(s) 166
FaeI CATG 5 cut(s) 112, 298, 567, 599, 854
FatI CATG 5 cut(s) 108, 294, 563, 595, 850
FauI CCCGC 1 cut(s) 275
Fnu4HI GCNGC 2 cut(s) 636, 1059
FokI GGATG 1 cut(s) 886
Fsp4HI GCNGC 2 cut(s) 636, 1059
FspBI CTAG 2 cut(s) 368, 689
GlaI GCGC 1 cut(s) 514
GluI GCNGC 2 cut(s) 636, 1059
GsaI CCCAGC 3 cut(s) 247, 765, 769
GsuI CTGGAG 1 cut(s) 262
HaeII RGCGCY 1 cut(s) 516
HaeIII GGCC 6 cut(s) 119, 150, 299, 560, 803, 824
HapII CCGG 2 cut(s) 174, 300
HhaI GCGC 1 cut(s) 515
Hin1II CATG 5 cut(s) 112, 298, 567, 599, 854
Hin6I GCGC 1 cut(s) 513
HinP1I GCGC 1 cut(s) 513
HinfI GANTC 2 cut(s) 25, 353
HpaII CCGG 2 cut(s) 174, 300
HphI GGTGA 3 cut(s) 460, 692, 879
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 2 cut(s) 376, 586
Hpy188III TCNNGA 6 cut(s) 41, 279, 830, 842, 863, 1013
Hpy8I GTNNAC 1 cut(s) 7
Hpy99I CGWCG 2 cut(s) 186, 293
HpyAV CCTTC 3 cut(s) 541, 733, 958
HpyCH4III ACNGT 2 cut(s) 11, 386
HpyCH4V TGCA 7 cut(s) 422, 675, 749, 755, 899, 959, 1058
HpyF10VI GCNNNNNNNGC 2 cut(s) 641, 956
HpyF3I CTNAG 3 cut(s) 99, 840, 951
Hsp92II CATG 5 cut(s) 112, 298, 567, 599, 854
HspAI GCGC 1 cut(s) 513
KroI GCCGGC 1 cut(s) 299
KroNI GCCGGC 1 cut(s) 301
Kzo9I GATC 4 cut(s) 185, 442, 466, 613
LmnI GCTCC 3 cut(s) 93, 197, 252
Lsp1109I GCAGC 1 cut(s) 622
LweI GCATC 3 cut(s) 294, 742, 908
MaeI CTAG 2 cut(s) 368, 689
MaeIII GTNAC 3 cut(s) 22, 885, 1039
MalI GATC 4 cut(s) 187, 444, 468, 615
MboI GATC 4 cut(s) 185, 442, 466, 613
MboII GAAGA 7 cut(s) 217, 227, 249, 463, 620, 704, 980
MfeI CAATTG 1 cut(s) 542
MhlI GDGCHC 1 cut(s) 194
MlsI TGGCCA 1 cut(s) 824
MluCI AATT 8 cut(s) 392, 490, 542, 600, 912, 943, 1016, 1033
MluNI TGGCCA 1 cut(s) 824
MlyI GAGTC 2 cut(s) 19, 347
MmeI TCCRAC 4 cut(s) 115, 180, 253, 481
Mox20I TGGCCA 1 cut(s) 824
MroNI GCCGGC 1 cut(s) 299
MscI TGGCCA 1 cut(s) 824
MseI TTAA 2 cut(s) 461, 1004
MslI CAYNNNNRTG 1 cut(s) 113
Msp20I TGGCCA 1 cut(s) 824
MspA1I CMGCKG 1 cut(s) 765
MspI CCGG 2 cut(s) 174, 300
MunI CAATTG 1 cut(s) 542
MwoI GCNNNNNNNGC 2 cut(s) 641, 956
NaeI GCCGGC 1 cut(s) 301
NcoI CCATGG 1 cut(s) 294
NdeII GATC 4 cut(s) 185, 442, 466, 613
NgoMIV GCCGGC 1 cut(s) 299
NlaIII CATG 5 cut(s) 112, 298, 567, 599, 854
NlaIV GGNNCC 2 cut(s) 559, 1011
NmuCI GTSAC 2 cut(s) 22, 885
NspI RCATGY 3 cut(s) 112, 599, 854
PciI ACATGT 1 cut(s) 850
PdiI GCCGGC 1 cut(s) 301
PkrI GCNGC 2 cut(s) 637, 1060
Ple19I CGATCG 1 cut(s) 188
PleI GAGTC 2 cut(s) 19, 347
PpsI GAGTC 2 cut(s) 19, 347
PpuMI RGGWCCY 1 cut(s) 1010
PscI ACATGT 1 cut(s) 850
Psp5II RGGWCCY 1 cut(s) 1010
PspFI CCCAGC 3 cut(s) 243, 761, 765
PspN4I GGNNCC 2 cut(s) 559, 1011
PspPI GGNCC 5 cut(s) 149, 202, 558, 801, 1010
PspPPI RGGWCCY 1 cut(s) 1010
PstNI CAGNNNCTG 1 cut(s) 736
PvuI CGATCG 1 cut(s) 188
PvuII CAGCTG 1 cut(s) 765
RsaI GTAC 2 cut(s) 759, 849
RsaNI GTAC 2 cut(s) 758, 848
RseI CAYNNNNRTG 1 cut(s) 113
SaqAI TTAA 2 cut(s) 461, 1004
SatI GCNGC 2 cut(s) 636, 1059
Sau3AI GATC 4 cut(s) 185, 442, 466, 613
Sau96I GGNCC 5 cut(s) 149, 202, 558, 801, 1010
SchI GAGTC 2 cut(s) 19, 347
SduI GDGCHC 1 cut(s) 194
SfaNI GCATC 3 cut(s) 294, 742, 908
SinI GGWCC 2 cut(s) 202, 1010
SmiMI CAYNNNNRTG 1 cut(s) 113
Sse9I AATT 8 cut(s) 392, 490, 542, 600, 912, 943, 1016, 1033
SsiI CCGC 2 cut(s) 250, 268
SspMI CTAG 2 cut(s) 368, 689
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 2 cut(s) 11, 386
TaqI TCGA 2 cut(s) 82, 441
TasI AATT 8 cut(s) 392, 490, 542, 600, 912, 943, 1016, 1033
TatI WGTACW 1 cut(s) 847
Tru1I TTAA 2 cut(s) 461, 1004
Tru9I TTAA 2 cut(s) 461, 1004
TscAI CASTG 3 cut(s) 103, 310, 391
TseFI GTSAC 2 cut(s) 22, 885
TseI GCWGC 2 cut(s) 635, 1058
Tsp45I GTSAC 2 cut(s) 22, 885
TspGWI ACGGA 3 cut(s) 148, 604, 1027
TspRI CASTG 3 cut(s) 103, 310, 391
VpaK11BI GGWCC 2 cut(s) 202, 1010
XapI RAATTY 2 cut(s) 490, 1016
XceI RCATGY 3 cut(s) 112, 599, 854
XspI CTAG 2 cut(s) 368, 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.