RLG00000008493

Triosephosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0009314
Physical Location & Seq
Forward (+)
31276300 .. 31279597
3298 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008493

Sequence Viewer

Length: 945 bp
ATGGCGGTGGCCTCCACATCTCTCGCCTCCCAACTCTCCGGCCCTAAATCTCTCTCCCAGTCCTACTCCGGCCTCCGACGATCGTGCCCCAAACTCGACCATTCCCACTCCCCTCGGTCCCTCTTCCAACACCTCCACTCCCACCTCAGCCTCTCCTCCTCCCGCAAGGCCTCCAGAGCCGTCGTCGCCATGGCCGGCACCGGAAAGTTTTTTGTTGGTGGAAACTGGAAGTGTAATGGCACAAAAGACTCAATCAGCAAGCTAGTGTCAGACTTAAACAGCGCAAAATTGGAACCTGATGTTGATGTTGTTGTAGCACCACCATTTCTTTACTTGGATCAGGTGAAGAGCTCACTAACAGATCGCATTGAGATATCTGGTCAAAATTCTTGGGTTGGAAAAGGTGGGGCTTTCACTGGGGAAATCAGTGTGGAACAATTGAAGGATATTGGCTGCAAATGGGTTATTCTTGGGCACTCTGAGCGGAGACATGTAATTGGTGAAGATGATCAGTTTATAGGAAAGAAAGCTGCCTATGCCTTGAATGAGGGTCTGGGAGTAATTGCTTGCATTGGTGAGAAGTTAGAAGAAAGGGAAGCCGGGAAAACTTTTGACGTCTGTTTCCAGCAACTGAAGGCTTTTGCAGATGCTGTACCTAGTTGGGAAAATATAGTTGTTGCTTACGAGCCTGTATGGGCCATTGGAACCGGTAAGGTGGCCTGTCCACAACAAGCTCAGGAAGTACATGTAGCAGTTCGTGATTGGCTCAAAAAGAATGTGTCAGCAGAAGTTGCATCTAAAACCAGAATTATTTATGGAGGATCTGTAAACGGAGGCAATTCTGCTGAGCTTGCAAAGGAGGAAGATATTGATGGGTTTCTAGTTGGTGGTGCTTCCTTGAAGGTATACTCCTTGCAACTTTTCACCTTGAAATTTCAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003674 GO:0003824 GO:0004807 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005975 GO:0005996 GO:0006006 GO:0006066 GO:0006071 GO:0006081 GO:0006082 GO:0006090 GO:0006091 GO:0006094 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006642 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009657 GO:0009658 GO:0009941 GO:0009987 GO:0016043 GO:0016051 GO:0016052 GO:0016053 GO:0016310 GO:0016853 GO:0016860 GO:0016861 GO:0017144 GO:0018130 GO:0019318 GO:0019319 GO:0019359 GO:0019362 GO:0019363 GO:0019400 GO:0019405 GO:0019438 GO:0019439 GO:0019563 GO:0019637 GO:0019682 GO:0019693 GO:0019751 GO:0019752 GO:0022622 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044262 GO:0044270 GO:0044271 GO:0044275 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046164 GO:0046166 GO:0046174 GO:0046184 GO:0046364 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046486 GO:0046496 GO:0046700 GO:0046939 GO:0048046 GO:0048364 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0071840 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0080022 GO:0090407 GO:0099402 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901616
KEGG Pathways
Metabolic & Signaling
ko00010 ko00051 ko00562 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00051 map00562 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
TIM

Protein Analysis

315

Amino Acids

33.9

Weight (kDa)

8.43

Isoelectric Point (pI)

38.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIM PF00121 71 - 304 6.1e-89 Triosephosphate isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 618
AccB1I GGYRCC 1 cut(s) 197
AccBSI CCGCTC 1 cut(s) 484
AccI GTMKAC 1 cut(s) 906
AciI CCGC 3 cut(s) 5, 163, 484
AclWI GGATC 2 cut(s) 345, 829
AcoI YGGCCR 1 cut(s) 192
AcsI RAATTY 2 cut(s) 385, 932
AcuI CTGAAG 1 cut(s) 653
AcyI GRCGYC 1 cut(s) 615
AfaI GTAC 2 cut(s) 654, 744
AflIII ACRYGT 2 cut(s) 490, 745
AgeI ACCGGT 1 cut(s) 707
AgsI TTSAA 5 cut(s) 442, 544, 901, 931, 938
AluBI AGCT 5 cut(s) 262, 351, 530, 734, 850
AluI AGCT 5 cut(s) 262, 351, 530, 734, 850
Alw21I GWGCWC 1 cut(s) 353
Alw26I GTCTC 1 cut(s) 481
AlwI GGATC 2 cut(s) 345, 829
AlwNI CAGNNNCTG 2 cut(s) 631, 650
AoxI GGCC 7 cut(s) 9, 40, 70, 168, 192, 696, 717
ApeKI GCWGC 2 cut(s) 453, 530
ApoI RAATTY 2 cut(s) 385, 932
AsiGI ACCGGT 1 cut(s) 707
AspLEI GCGC 1 cut(s) 284
AspS9I GGNCC 3 cut(s) 41, 117, 696
AsuC2I CCSGG 1 cut(s) 601
AsuHPI GGTGA 4 cut(s) 355, 512, 587, 916
AvaII GGWCC 1 cut(s) 117
BaeGI GKGCMC 2 cut(s) 89, 477
BanI GGYRCC 1 cut(s) 197
BanII GRGCYC 1 cut(s) 353
Bbv12I GWGCWC 1 cut(s) 353
BbvCI CCTCAGC 1 cut(s) 146
BbvI GCAGC 2 cut(s) 440, 517
BccI CCATC 1 cut(s) 866
BceAI ACGGC 1 cut(s) 164
BcgI CGANNNNNNTGC 2 cut(s) 66, 100
BclI TGATCA 1 cut(s) 508
BcnI CCSGG 1 cut(s) 601
BcoDI GTCTC 1 cut(s) 481
BfaI CTAG 3 cut(s) 263, 657, 881
BisI GCNGC 2 cut(s) 454, 531
BlpI GCTNAGC 1 cut(s) 846
BlsI GCNGC 2 cut(s) 455, 532
Bme1390I CCNGG 1 cut(s) 601
Bme18I GGWCC 1 cut(s) 117
BmgT120I GGNCC 3 cut(s) 41, 117, 696
BmiI GGNNCC 4 cut(s) 119, 199, 294, 706
BmrFI CCNGG 1 cut(s) 601
BmrI ACTGGG 2 cut(s) 52, 426
BmsI GCATC 2 cut(s) 637, 803
BmuI ACTGGG 2 cut(s) 52, 426
BpmI CTGGAG 1 cut(s) 157
Bpu10I CCTNAGC 2 cut(s) 146, 735
Bpu1102I GCTNAGC 1 cut(s) 846
BpuMI CCSGG 1 cut(s) 601
BsaHI GRCGYC 1 cut(s) 615
BsaJI CCNNGG 2 cut(s) 113, 189
BsaWI WCCGGW 2 cut(s) 200, 707
BsaXI ACNNNNNCTCC 4 cut(s) 122, 152, 810, 840
Bse118I RCCGGY 2 cut(s) 194, 707
Bse1I ACTGG 3 cut(s) 58, 230, 421
BseDI CCNNGG 2 cut(s) 113, 189
BseMII CTCAG 4 cut(s) 160, 471, 749, 837
BseNI ACTGG 3 cut(s) 58, 230, 421
BseRI GAGGAG 2 cut(s) 145, 148
BseSI GKGCMC 2 cut(s) 89, 477
BseXI GCAGC 2 cut(s) 440, 517
Bsh1285I CGRYCG 1 cut(s) 83
BshFI GGCC 7 cut(s) 11, 42, 72, 170, 194, 698, 719
BshNI GGYRCC 1 cut(s) 197
BshTI ACCGGT 1 cut(s) 707
BsiEI CGRYCG 1 cut(s) 83
BsiHKAI GWGCWC 1 cut(s) 353
BsiSI CCGG 6 cut(s) 39, 69, 195, 201, 600, 708
BslFI GGGAC 1 cut(s) 103
BsmAI GTCTC 1 cut(s) 481
BsmFI GGGAC 1 cut(s) 103
BsnI GGCC 7 cut(s) 11, 42, 72, 170, 194, 698, 719
Bsp1286I GDGCHC 3 cut(s) 89, 353, 477
Bsp143I GATC 5 cut(s) 80, 337, 361, 508, 821
Bsp1720I GCTNAGC 1 cut(s) 846
Bsp19I CCATGG 1 cut(s) 189
BspACI CCGC 3 cut(s) 5, 163, 484
BspANI GGCC 7 cut(s) 11, 42, 72, 170, 194, 698, 719
BspCNI CTCAG 4 cut(s) 159, 472, 748, 838
BspLI GGNNCC 4 cut(s) 119, 199, 294, 706
BspPI GGATC 2 cut(s) 345, 829
BspQI GCTCTTC 1 cut(s) 341
BspT107I GGYRCC 1 cut(s) 197
BsrBI CCGCTC 1 cut(s) 484
BsrFI RCCGGY 2 cut(s) 194, 707
BsrI ACTGG 3 cut(s) 58, 230, 421
BssAI RCCGGY 2 cut(s) 194, 707
BssECI CCNNGG 2 cut(s) 113, 189
BssMI GATC 5 cut(s) 80, 337, 361, 508, 821
BssNAI GTATAC 1 cut(s) 907
BssNI GRCGYC 1 cut(s) 615
BssT1I CCWWGG 1 cut(s) 189
Bst1107I GTATAC 1 cut(s) 907
Bst6I CTCTTC 2 cut(s) 128, 341
BstACI GRCGYC 1 cut(s) 615
BstAPI GCANNNNNTGC 1 cut(s) 791
BstC8I GCNNGC 4 cut(s) 196, 260, 568, 852
BstDEI CTNAG 4 cut(s) 146, 480, 735, 846
BstDSI CCRYGG 1 cut(s) 189
BstHHI GCGC 1 cut(s) 284
BstKTI GATC 5 cut(s) 83, 340, 364, 511, 824
BstMAI GTCTC 1 cut(s) 481
BstMBI GATC 5 cut(s) 80, 337, 361, 508, 821
BstMCI CGRYCG 1 cut(s) 83
BstMWI GCNNNNNNNGC 6 cut(s) 176, 185, 481, 536, 791, 851
BstNSI RCATGY 2 cut(s) 494, 749
BstSCI CCNGG 1 cut(s) 599
BstSLI GKGCMC 2 cut(s) 89, 477
BstV1I GCAGC 2 cut(s) 440, 517
BstX2I RGATCY 1 cut(s) 821
BstYI RGATCY 1 cut(s) 821
BstZ17I GTATAC 1 cut(s) 907
BsuRI GGCC 7 cut(s) 11, 42, 72, 170, 194, 698, 719
BtgI CCRYGG 1 cut(s) 189
BtsIMutI CAGTG 2 cut(s) 414, 433
Cac8I GCNNGC 4 cut(s) 196, 260, 568, 852
CaiI CAGNNNCTG 2 cut(s) 631, 650
CfoI GCGC 1 cut(s) 284
Cfr10I RCCGGY 2 cut(s) 194, 707
Cfr13I GGNCC 3 cut(s) 41, 117, 696
Csp6I GTAC 2 cut(s) 653, 743
CspAI ACCGGT 1 cut(s) 707
CviAII CATG 3 cut(s) 190, 491, 746
CviQI GTAC 2 cut(s) 653, 743
DdeI CTNAG 4 cut(s) 146, 480, 735, 846
DpnI GATC 5 cut(s) 82, 339, 363, 510, 823
DpnII GATC 5 cut(s) 80, 337, 361, 508, 821
EaeI YGGCCR 1 cut(s) 192
Eam1104I CTCTTC 2 cut(s) 128, 341
EarI CTCTTC 2 cut(s) 128, 341
Ecl136II GAGCTC 1 cut(s) 351
Eco130I CCWWGG 1 cut(s) 189
Eco147I AGGCCT 1 cut(s) 170
Eco24I GRGCYC 1 cut(s) 353
Eco32I GATATC 1 cut(s) 375
Eco47I GGWCC 1 cut(s) 117
Eco53kI GAGCTC 1 cut(s) 351
Eco57I CTGAAG 1 cut(s) 653
EcoICRI GAGCTC 1 cut(s) 351
EcoRV GATATC 1 cut(s) 375
EcoT14I CCWWGG 1 cut(s) 189
EcoT38I GRGCYC 1 cut(s) 353
ErhI CCWWGG 1 cut(s) 189
FaeI CATG 3 cut(s) 193, 494, 749
FaiI YATR 9 cut(s) 191, 492, 518, 537, 671, 694, 747, 816, 907
FaqI GGGAC 1 cut(s) 103
FatI CATG 3 cut(s) 189, 490, 745
FauI CCCGC 1 cut(s) 170
FbaI TGATCA 1 cut(s) 508
FblI GTMKAC 1 cut(s) 906
Fnu4HI GCNGC 2 cut(s) 454, 531
FriOI GRGCYC 1 cut(s) 353
Fsp4HI GCNGC 2 cut(s) 454, 531
FspBI CTAG 3 cut(s) 263, 657, 881
GlaI GCGC 1 cut(s) 283
GluI GCNGC 2 cut(s) 454, 531
GsuI CTGGAG 1 cut(s) 157
HaeIII GGCC 7 cut(s) 11, 42, 72, 170, 194, 698, 719
HapII CCGG 6 cut(s) 39, 69, 195, 201, 600, 708
HhaI GCGC 1 cut(s) 284
Hin1I GRCGYC 1 cut(s) 615
Hin1II CATG 3 cut(s) 193, 494, 749
Hin6I GCGC 1 cut(s) 282
HinP1I GCGC 1 cut(s) 282
HinfI GANTC 1 cut(s) 248
HpaII CCGG 6 cut(s) 39, 69, 195, 201, 600, 708
HphI GGTGA 4 cut(s) 355, 512, 587, 916
Hpy166II GTNNAC 3 cut(s) 725, 829, 907
Hpy188I TCNGA 3 cut(s) 77, 271, 481
Hpy188III TCNNGA 3 cut(s) 174, 737, 758
Hpy8I GTNNAC 3 cut(s) 725, 829, 907
Hpy99I CGWCG 3 cut(s) 81, 185, 188
HpyAV CCTTC 3 cut(s) 436, 628, 895
HpyCH4IV ACGT 1 cut(s) 615
HpyCH4V TGCA 6 cut(s) 456, 570, 644, 794, 854, 916
HpyF10VI GCNNNNNNNGC 6 cut(s) 176, 185, 481, 536, 791, 851
HpyF3I CTNAG 4 cut(s) 146, 480, 735, 846
HpySE526I ACGT 1 cut(s) 615
Hsp92I GRCGYC 1 cut(s) 615
Hsp92II CATG 3 cut(s) 193, 494, 749
HspAI GCGC 1 cut(s) 282
KroI GCCGGC 1 cut(s) 194
KroNI GCCGGC 1 cut(s) 196
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 5 cut(s) 80, 337, 361, 508, 821
LguI GCTCTTC 1 cut(s) 341
Lsp1109I GCAGC 2 cut(s) 440, 517
LweI GCATC 2 cut(s) 637, 803
MaeI CTAG 3 cut(s) 263, 657, 881
MaeII ACGT 1 cut(s) 615
MalI GATC 5 cut(s) 82, 339, 363, 510, 823
MbiI CCGCTC 1 cut(s) 484
MboI GATC 5 cut(s) 80, 337, 361, 508, 821
MboII GAAGA 5 cut(s) 115, 358, 515, 599, 875
MfeI CAATTG 1 cut(s) 437
MflI RGATCY 1 cut(s) 821
MhlI GDGCHC 3 cut(s) 89, 353, 477
MluCI AATT 8 cut(s) 287, 385, 437, 495, 561, 807, 838, 932
MlyI GAGTC 1 cut(s) 242
MmeI TCCRAC 3 cut(s) 100, 151, 376
MroNI GCCGGC 1 cut(s) 194
MseI TTAA 1 cut(s) 275
MspI CCGG 6 cut(s) 39, 69, 195, 201, 600, 708
MspR9I CCNGG 1 cut(s) 601
MunI CAATTG 1 cut(s) 437
MwoI GCNNNNNNNGC 6 cut(s) 176, 185, 481, 536, 791, 851
NaeI GCCGGC 1 cut(s) 196
NciI CCSGG 1 cut(s) 601
NcoI CCATGG 1 cut(s) 189
NdeII GATC 5 cut(s) 80, 337, 361, 508, 821
NgoMIV GCCGGC 1 cut(s) 194
NlaIII CATG 3 cut(s) 193, 494, 749
NlaIV GGNNCC 4 cut(s) 119, 199, 294, 706
NspI RCATGY 2 cut(s) 494, 749
PceI AGGCCT 1 cut(s) 170
PciI ACATGT 2 cut(s) 490, 745
PciSI GCTCTTC 1 cut(s) 341
PdiI GCCGGC 1 cut(s) 196
PinAI ACCGGT 1 cut(s) 707
PkrI GCNGC 2 cut(s) 455, 532
Ple19I CGATCG 1 cut(s) 83
PleI GAGTC 1 cut(s) 242
PpsI GAGTC 1 cut(s) 242
PscI ACATGT 2 cut(s) 490, 745
Psp124BI GAGCTC 1 cut(s) 353
PspN4I GGNNCC 4 cut(s) 119, 199, 294, 706
PspPI GGNCC 3 cut(s) 41, 117, 696
PstNI CAGNNNCTG 2 cut(s) 631, 650
PsuI RGATCY 1 cut(s) 821
PvuI CGATCG 1 cut(s) 83
RsaI GTAC 2 cut(s) 654, 744
RsaNI GTAC 2 cut(s) 653, 743
SacI GAGCTC 1 cut(s) 353
SapI GCTCTTC 1 cut(s) 341
SaqAI TTAA 1 cut(s) 275
SatI GCNGC 2 cut(s) 454, 531
Sau3AI GATC 5 cut(s) 80, 337, 361, 508, 821
Sau96I GGNCC 3 cut(s) 41, 117, 696
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 1 cut(s) 601
SduI GDGCHC 3 cut(s) 89, 353, 477
SfaNI GCATC 2 cut(s) 637, 803
SinI GGWCC 1 cut(s) 117
Sse9I AATT 8 cut(s) 287, 385, 437, 495, 561, 807, 838, 932
SseBI AGGCCT 1 cut(s) 170
SsiI CCGC 3 cut(s) 5, 163, 484
SspMI CTAG 3 cut(s) 263, 657, 881
SstI GAGCTC 1 cut(s) 353
StuI AGGCCT 1 cut(s) 170
StyD4I CCNGG 1 cut(s) 599
StyI CCWWGG 1 cut(s) 189
TaiI ACGT 1 cut(s) 618
TaqI TCGA 1 cut(s) 96
TaqII GACCGA 1 cut(s) 105
TasI AATT 8 cut(s) 287, 385, 437, 495, 561, 807, 838, 932
TatI WGTACW 1 cut(s) 742
Tru1I TTAA 1 cut(s) 275
Tru9I TTAA 1 cut(s) 275
TscAI CASTG 2 cut(s) 421, 433
TseI GCWGC 2 cut(s) 453, 530
TspGWI ACGGA 1 cut(s) 846
TspRI CASTG 2 cut(s) 421, 433
VpaK11BI GGWCC 1 cut(s) 117
XapI RAATTY 2 cut(s) 385, 932
XceI RCATGY 2 cut(s) 494, 749
XmiI GTMKAC 1 cut(s) 906
XspI CTAG 3 cut(s) 263, 657, 881
ZraI GACGTC 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.