MD10G1118200.v1.1

Triosephosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009314
Physical Location & Seq
Reverse (-)
19549477 .. 19552985
3509 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1118200.v1.1.491

Sequence Viewer

Length: 969 bp
ATGGCGGTGGCCTCCACATCTCTCGCCTCCCAACTCTCCGGCCCTAAATCCCTCTCCTCTTACTCCGGCCTCCGTCCGTCGTGCTGCAAACTCGAATTCTCTCACTCTCTCTCCGCCACCCAATCCCTCTTCCGGCACCTTCACTCCCTCCTCCGCCTCTCCTCCTCCTCTCGCAAGGCCTCCAGAGGCGTCGTCGCCATGGCCGGCACCGGAAAGTTTTTTGTTGGTGGAAACTGGAAGTGTAATGGCACAAAAGACTCCATAAGAAAGCTAGTCGCTGACTTGAACAGCGCAAAATTGGAAGCGGATGTTGATGTTGTTGTAGCACCACCATTTCTTTACTTAGATCAGGTGAAGAGCTCATTAACAGATTGTATTGAATTATCTGGTCAAAATTCTTGGGTTGGAAAAGGTGGGGCCTTCACGGGAGAAATCAGTGTGGAACAACTGAAGGATATTGGAGCCACATGGGTTATTCTTGGGCACTCAGAACGGAGACATGTGATTGGTGAAGACGATCAGTTTATAGGAAAAAAAGCTGCCTATGCCTTGAGTGAGGGCCTTGGAGTAATTGCTTGCATCGGTGAGAAGCTAGAAGAAAGGGAAGCGGGTAAAACTTTTGACGTCTGCTTTCAGCAACTGAAGGCTTTTGCTGACGCAGTACCAAGCTGGGATAAAATAGTTGTTGCTTATGAGCCTGTATGGGCCATTGGGACTGGTAAGGTGGCCAGTCCAGAACAAGCTCAGGAAGTACATGTAGCTGTTCGCGATTGGCTGAAACAGAACGTGTCAGCAGAAGTTGCATCTAAAACAAGAATTATTTATGGAGGGTCTGTAAATGGAGGCAATTCTGCTGAGCTCGCGAAGAAGGAAGATATTGATGGTTTTCTTGTTGGCGGTGCATCCTTAAAGGGTCCTGAATTCGCTACTATCGTCAACTCTGTGACAGCCAAGAAAGTTGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003674 GO:0003824 GO:0004807 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005975 GO:0005996 GO:0006006 GO:0006066 GO:0006071 GO:0006081 GO:0006082 GO:0006090 GO:0006091 GO:0006094 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006642 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009657 GO:0009658 GO:0009941 GO:0009987 GO:0016043 GO:0016051 GO:0016052 GO:0016053 GO:0016310 GO:0016853 GO:0016860 GO:0016861 GO:0017144 GO:0018130 GO:0019318 GO:0019319 GO:0019359 GO:0019362 GO:0019363 GO:0019400 GO:0019405 GO:0019438 GO:0019439 GO:0019563 GO:0019637 GO:0019682 GO:0019693 GO:0019751 GO:0019752 GO:0022622 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044262 GO:0044270 GO:0044271 GO:0044275 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046164 GO:0046166 GO:0046174 GO:0046184 GO:0046364 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046486 GO:0046496 GO:0046700 GO:0046939 GO:0048046 GO:0048364 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0071840 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0080022 GO:0090407 GO:0099402 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901616
KEGG Pathways
Metabolic & Signaling
ko00010 ko00051 ko00562 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00051 map00562 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
TIM

Protein Analysis

323

Amino Acids

34.17

Weight (kDa)

8.13

Isoelectric Point (pI)

29.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIM PF00121 74 - 314 1.6e-90 Triosephosphate isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 627
AccB1I GGYRCC 2 cut(s) 135, 206
AccII CGCG 2 cut(s) 768, 863
AciI CCGC 6 cut(s) 5, 114, 154, 305, 608, 897
AcoI YGGCCR 2 cut(s) 201, 726
AcsI RAATTY 3 cut(s) 95, 394, 920
AcuI CTGAAG 2 cut(s) 470, 662
AcyI GRCGYC 2 cut(s) 189, 624
AfaI GTAC 2 cut(s) 663, 753
AfiI CCNNNNNNNGG 1 cut(s) 132
AflIII ACRYGT 3 cut(s) 499, 754, 786
AgsI TTSAA 2 cut(s) 286, 380
AluBI AGCT 8 cut(s) 271, 360, 539, 592, 669, 743, 761, 859
AluI AGCT 8 cut(s) 271, 360, 539, 592, 669, 743, 761, 859
Alw21I GWGCWC 2 cut(s) 362, 861
Alw26I GTCTC 1 cut(s) 490
AlwNI CAGNNNCTG 1 cut(s) 640
AoxI GGCC 9 cut(s) 9, 40, 67, 177, 201, 417, 559, 705, 726
ApeKI GCWGC 3 cut(s) 84, 539, 962
ApoI RAATTY 3 cut(s) 95, 394, 920
AspLEI GCGC 1 cut(s) 293
AspS9I GGNCC 5 cut(s) 41, 417, 559, 705, 914
AsuHPI GGTGA 3 cut(s) 364, 521, 596
AvaII GGWCC 1 cut(s) 914
BaeGI GKGCMC 1 cut(s) 486
BalI TGGCCA 1 cut(s) 728
BanI GGYRCC 2 cut(s) 135, 206
BanII GRGCYC 2 cut(s) 362, 861
BbsI GAAGAC 1 cut(s) 519
Bbv12I GWGCWC 2 cut(s) 362, 861
BbvI GCAGC 3 cut(s) 71, 526, 949
BccI CCATC 1 cut(s) 875
BcoDI GTCTC 1 cut(s) 490
BfaI CTAG 2 cut(s) 272, 593
BisI GCNGC 3 cut(s) 85, 540, 963
BlpI GCTNAGC 1 cut(s) 855
BlsI GCNGC 3 cut(s) 86, 541, 964
Bme18I GGWCC 1 cut(s) 914
BmgT120I GGNCC 5 cut(s) 41, 417, 559, 705, 914
BmiI GGNNCC 5 cut(s) 137, 208, 418, 463, 915
BmsI GCATC 3 cut(s) 588, 812, 911
BpiI GAAGAC 1 cut(s) 519
BpmI CTGGAG 1 cut(s) 166
Bpu10I CCTNAGC 1 cut(s) 744
Bpu1102I GCTNAGC 1 cut(s) 855
BpuEI CTTGAG 1 cut(s) 571
BsaHI GRCGYC 2 cut(s) 189, 624
BsaJI CCNNGG 2 cut(s) 198, 562
BsaWI WCCGGW 1 cut(s) 209
BsaXI ACNNNNNCTCC 6 cut(s) 95, 125, 128, 158, 819, 849
Bsc4I CCNNNNNNNGG 1 cut(s) 132
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 3 cut(s) 239, 721, 729
BseDI CCNNGG 2 cut(s) 198, 562
BseGI GGATG 2 cut(s) 313, 902
BseLI CCNNNNNNNGG 1 cut(s) 132
BseMII CTCAG 3 cut(s) 501, 758, 846
BseNI ACTGG 3 cut(s) 239, 721, 729
BseRI GAGGAG 5 cut(s) 46, 140, 151, 154, 157
BseSI GKGCMC 1 cut(s) 486
BseXI GCAGC 3 cut(s) 71, 526, 949
BseYI CCCAGC 1 cut(s) 669
Bsh1236I CGCG 2 cut(s) 768, 863
BshFI GGCC 9 cut(s) 11, 42, 69, 179, 203, 419, 561, 707, 728
BshNI GGYRCC 2 cut(s) 135, 206
BsiHKAI GWGCWC 2 cut(s) 362, 861
BsiSI CCGG 5 cut(s) 39, 66, 133, 204, 210
BslFI GGGAC 1 cut(s) 727
BslI CCNNNNNNNGG 1 cut(s) 132
BsmAI GTCTC 1 cut(s) 490
BsmFI GGGAC 1 cut(s) 727
BsnI GGCC 9 cut(s) 11, 42, 69, 179, 203, 419, 561, 707, 728
Bsp1286I GDGCHC 3 cut(s) 362, 486, 861
Bsp143I GATC 2 cut(s) 346, 517
Bsp1720I GCTNAGC 1 cut(s) 855
Bsp19I CCATGG 1 cut(s) 198
Bsp68I TCGCGA 2 cut(s) 768, 863
BspACI CCGC 6 cut(s) 5, 114, 154, 305, 608, 897
BspANI GGCC 9 cut(s) 11, 42, 69, 179, 203, 419, 561, 707, 728
BspCNI CTCAG 3 cut(s) 500, 757, 847
BspFNI CGCG 2 cut(s) 768, 863
BspLI GGNNCC 5 cut(s) 137, 208, 418, 463, 915
BspQI GCTCTTC 1 cut(s) 350
BspT107I GGYRCC 2 cut(s) 135, 206
BsrFI RCCGGY 1 cut(s) 203
BsrI ACTGG 3 cut(s) 239, 721, 729
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 2 cut(s) 198, 562
BssMI GATC 2 cut(s) 346, 517
BssNI GRCGYC 2 cut(s) 189, 624
BssT1I CCWWGG 2 cut(s) 198, 562
Bst6I CTCTTC 2 cut(s) 134, 350
BstACI GRCGYC 2 cut(s) 189, 624
BstAPI GCANNNNNTGC 1 cut(s) 800
BstC8I GCNNGC 3 cut(s) 205, 577, 861
BstDEI CTNAG 4 cut(s) 343, 487, 744, 855
BstDSI CCRYGG 1 cut(s) 198
BstF5I GGATG 2 cut(s) 313, 902
BstFNI CGCG 2 cut(s) 768, 863
BstHHI GCGC 1 cut(s) 293
BstKTI GATC 2 cut(s) 349, 520
BstMAI GTCTC 1 cut(s) 490
BstMBI GATC 2 cut(s) 346, 517
BstMWI GCNNNNNNNGC 3 cut(s) 545, 800, 860
BstNSI RCATGY 2 cut(s) 503, 758
BstSLI GKGCMC 1 cut(s) 486
BstUI CGCG 2 cut(s) 768, 863
BstV1I GCAGC 3 cut(s) 71, 526, 949
BstV2I GAAGAC 1 cut(s) 519
BsuRI GGCC 9 cut(s) 11, 42, 69, 179, 203, 419, 561, 707, 728
BtgI CCRYGG 1 cut(s) 198
BtsCI GGATG 2 cut(s) 313, 902
BtsIMutI CAGTG 1 cut(s) 442
BtuMI TCGCGA 2 cut(s) 768, 863
Cac8I GCNNGC 3 cut(s) 205, 577, 861
CaiI CAGNNNCTG 1 cut(s) 640
CfoI GCGC 1 cut(s) 293
Cfr10I RCCGGY 1 cut(s) 203
Cfr13I GGNCC 5 cut(s) 41, 417, 559, 705, 914
CseI GACGC 2 cut(s) 178, 665
Csp6I GTAC 2 cut(s) 662, 752
CviAII CATG 4 cut(s) 199, 468, 500, 755
CviQI GTAC 2 cut(s) 662, 752
DdeI CTNAG 4 cut(s) 343, 487, 744, 855
DpnI GATC 2 cut(s) 348, 519
DpnII GATC 2 cut(s) 346, 517
EaeI YGGCCR 2 cut(s) 201, 726
Eam1104I CTCTTC 2 cut(s) 134, 350
EarI CTCTTC 2 cut(s) 134, 350
EciI GGCGGA 2 cut(s) 103, 143
Ecl136II GAGCTC 2 cut(s) 360, 859
Eco130I CCWWGG 2 cut(s) 198, 562
Eco147I AGGCCT 1 cut(s) 179
Eco24I GRGCYC 2 cut(s) 362, 861
Eco47I GGWCC 1 cut(s) 914
Eco53kI GAGCTC 2 cut(s) 360, 859
Eco57I CTGAAG 2 cut(s) 470, 662
EcoICRI GAGCTC 2 cut(s) 360, 859
EcoO109I RGGNCCY 3 cut(s) 417, 559, 914
EcoRI GAATTC 2 cut(s) 95, 920
EcoT14I CCWWGG 2 cut(s) 198, 562
EcoT38I GRGCYC 2 cut(s) 362, 861
ErhI CCWWGG 2 cut(s) 198, 562
FaeI CATG 4 cut(s) 202, 471, 503, 758
FaqI GGGAC 1 cut(s) 727
FatI CATG 4 cut(s) 198, 467, 499, 754
FauI CCCGC 1 cut(s) 601
Fnu4HI GCNGC 3 cut(s) 85, 540, 963
FokI GGATG 2 cut(s) 320, 889
FriOI GRGCYC 2 cut(s) 362, 861
Fsp4HI GCNGC 3 cut(s) 85, 540, 963
FspBI CTAG 2 cut(s) 272, 593
GlaI GCGC 1 cut(s) 292
GluI GCNGC 3 cut(s) 85, 540, 963
GsaI CCCAGC 1 cut(s) 673
GsuI CTGGAG 1 cut(s) 166
HaeIII GGCC 9 cut(s) 11, 42, 69, 179, 203, 419, 561, 707, 728
HapII CCGG 5 cut(s) 39, 66, 133, 204, 210
HgaI GACGC 2 cut(s) 178, 665
HhaI GCGC 1 cut(s) 293
Hin1I GRCGYC 2 cut(s) 189, 624
Hin1II CATG 4 cut(s) 202, 471, 503, 758
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HincII GTYRAC 1 cut(s) 937
HindII GTYRAC 1 cut(s) 937
HinfI GANTC 1 cut(s) 257
HpaII CCGG 5 cut(s) 39, 66, 133, 204, 210
HphI GGTGA 3 cut(s) 364, 521, 596
Hpy166II GTNNAC 1 cut(s) 937
Hpy188I TCNGA 1 cut(s) 490
Hpy188III TCNNGA 6 cut(s) 183, 734, 746, 767, 862, 917
Hpy8I GTNNAC 1 cut(s) 937
Hpy99I CGWCG 3 cut(s) 82, 194, 197
HpyAV CCTTC 5 cut(s) 149, 430, 445, 637, 862
HpyCH4IV ACGT 2 cut(s) 624, 786
HpyCH4V TGCA 4 cut(s) 87, 579, 803, 902
HpyF10VI GCNNNNNNNGC 3 cut(s) 545, 800, 860
HpyF3I CTNAG 4 cut(s) 343, 487, 744, 855
HpySE526I ACGT 2 cut(s) 624, 786
Hsp92I GRCGYC 2 cut(s) 189, 624
Hsp92II CATG 4 cut(s) 202, 471, 503, 758
HspAI GCGC 1 cut(s) 291
KroI GCCGGC 1 cut(s) 203
KroNI GCCGGC 1 cut(s) 205
Kzo9I GATC 2 cut(s) 346, 517
LguI GCTCTTC 1 cut(s) 350
LmnI GCTCC 1 cut(s) 461
Lsp1109I GCAGC 3 cut(s) 71, 526, 949
LweI GCATC 3 cut(s) 588, 812, 911
MaeI CTAG 2 cut(s) 272, 593
MaeII ACGT 2 cut(s) 624, 786
MaeIII GTNAC 1 cut(s) 943
MalI GATC 2 cut(s) 348, 519
MboI GATC 2 cut(s) 346, 517
MboII GAAGA 6 cut(s) 121, 367, 524, 608, 877, 884
MhlI GDGCHC 3 cut(s) 362, 486, 861
MlsI TGGCCA 1 cut(s) 728
MluCI AATT 8 cut(s) 95, 296, 380, 394, 570, 816, 847, 920
MluNI TGGCCA 1 cut(s) 728
MlyI GAGTC 1 cut(s) 251
MmeI TCCRAC 1 cut(s) 385
Mox20I TGGCCA 1 cut(s) 728
MroNI GCCGGC 1 cut(s) 203
MscI TGGCCA 1 cut(s) 728
MseI TTAA 2 cut(s) 365, 908
Msp20I TGGCCA 1 cut(s) 728
MspI CCGG 5 cut(s) 39, 66, 133, 204, 210
MvnI CGCG 2 cut(s) 768, 863
MwoI GCNNNNNNNGC 3 cut(s) 545, 800, 860
NaeI GCCGGC 1 cut(s) 205
NcoI CCATGG 1 cut(s) 198
NdeII GATC 2 cut(s) 346, 517
NgoMIV GCCGGC 1 cut(s) 203
NlaIII CATG 4 cut(s) 202, 471, 503, 758
NlaIV GGNNCC 5 cut(s) 137, 208, 418, 463, 915
NmuCI GTSAC 1 cut(s) 943
NruI TCGCGA 2 cut(s) 768, 863
NspI RCATGY 2 cut(s) 503, 758
PceI AGGCCT 1 cut(s) 179
PciI ACATGT 2 cut(s) 499, 754
PciSI GCTCTTC 1 cut(s) 350
PcsI WCGNNNNNNNCGW 1 cut(s) 930
PdiI GCCGGC 1 cut(s) 205
PkrI GCNGC 3 cut(s) 86, 541, 964
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
PpuMI RGGWCCY 1 cut(s) 914
PscI ACATGT 2 cut(s) 499, 754
Psp124BI GAGCTC 2 cut(s) 362, 861
Psp5II RGGWCCY 1 cut(s) 914
PspFI CCCAGC 1 cut(s) 669
PspN4I GGNNCC 5 cut(s) 137, 208, 418, 463, 915
PspPI GGNCC 5 cut(s) 41, 417, 559, 705, 914
PspPPI RGGWCCY 1 cut(s) 914
PstNI CAGNNNCTG 1 cut(s) 640
RruI TCGCGA 2 cut(s) 768, 863
RsaI GTAC 2 cut(s) 663, 753
RsaNI GTAC 2 cut(s) 662, 752
SacI GAGCTC 2 cut(s) 362, 861
SapI GCTCTTC 1 cut(s) 350
SaqAI TTAA 2 cut(s) 365, 908
SatI GCNGC 3 cut(s) 85, 540, 963
Sau3AI GATC 2 cut(s) 346, 517
Sau96I GGNCC 5 cut(s) 41, 417, 559, 705, 914
SchI GAGTC 1 cut(s) 251
SduI GDGCHC 3 cut(s) 362, 486, 861
SfaNI GCATC 3 cut(s) 588, 812, 911
SinI GGWCC 1 cut(s) 914
SmlI CTYRAG 1 cut(s) 550
SmoI CTYRAG 1 cut(s) 550
Sse9I AATT 8 cut(s) 95, 296, 380, 394, 570, 816, 847, 920
SseBI AGGCCT 1 cut(s) 179
SsiI CCGC 6 cut(s) 5, 114, 154, 305, 608, 897
SspMI CTAG 2 cut(s) 272, 593
SstI GAGCTC 2 cut(s) 362, 861
StuI AGGCCT 1 cut(s) 179
StyI CCWWGG 2 cut(s) 198, 562
TaiI ACGT 2 cut(s) 627, 789
TaqI TCGA 1 cut(s) 93
TasI AATT 8 cut(s) 95, 296, 380, 394, 570, 816, 847, 920
TatI WGTACW 1 cut(s) 751
Tru1I TTAA 2 cut(s) 365, 908
Tru9I TTAA 2 cut(s) 365, 908
TscAI CASTG 1 cut(s) 442
TseFI GTSAC 1 cut(s) 943
TseI GCWGC 3 cut(s) 84, 539, 962
Tsp45I GTSAC 1 cut(s) 943
TspGWI ACGGA 3 cut(s) 62, 66, 508
TspRI CASTG 1 cut(s) 442
VpaK11BI GGWCC 1 cut(s) 914
XapI RAATTY 3 cut(s) 95, 394, 920
XceI RCATGY 2 cut(s) 503, 758
XspI CTAG 2 cut(s) 272, 593
ZraI GACGTC 1 cut(s) 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.