FvH4_2g02030

Triosephosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009314
Physical Location & Seq
Forward (+)
1705935 .. 1709581
3647 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g02030.t1

Sequence Viewer

Length: 957 bp
ATGGCGGTGGCCTCCACATCTCTCGCCTCCCAACTCTCCGGCCCTAAATCCCTGTCGCAGTCCTACTCCGGCCTCCGACCATCCTGCCCCAAACTCGACCAGTCTCTTTCCCAAACTCACTCCTCCCTCTTCCAACACCTCAGCCTCTCCTCCTCCTCCCGCAAGGCCTCCAGAGCCGTCGTCGCCATGGCCGGAACCGGAAAGTTTTTTGTTGGTGGAAACTGGAAATGTAATGGCACAAAAGACTCGATCAGCAAGCTAGTGTCAGACTTAAACAGCGCAAAATTGGAACCTGATGTTGATGTTGTTGTAGCACCACCATTTCTTTACTTGGATCAGGTGAAAAGCTCACTAACAGATCGCATTGAGATATCCGGTCAAAATTCTTGGGTCGGAAAAGGTGGGGCTTTCACAGGGGAAATCAGTGTGGAACAATTGAAGGATATTGGCTGCAAATGGGTTATTCTTGGGCACTCTGAACGGAGACATGTAATTGGTGAAGATGATCAGTTTATAGGAAAGAAAGCTGCCTATGCCTTGAATGAGGGTCTGGGAGTAATTGCTTGCATTGGTGAGAAGTTAGAAGAAAGGGAAGCAGGGAAAACTTTTGACGTATGTTATGAGCAACTGAAGGCTTTTGCAGATGCTGTACCTAGCTGGGAAAATATAGTTGTTGCTTACGAGCCTGTATGGGCCATTGGAACTGGTAAGGTGGCCAGTCCACAACAAGCTCAGGAAGTACATGTAGCAGTTCGTGAGTGGCTCAAAAAGAATGTGTCAGCAGAGGTGGCATCTAAAACCAGAATTATTTATGGAGGATCTGTAAACGGAGGCAACTCTGCTGAGCTTGCAAAGGAGGAAGATATTGATGGGTTTCTAGTTGGTGGTGCTTCCTTGAAGGGACCTGAATTTGCTACCATTGTCAATGCTGTAACATCCAAGAAAGTTGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003674 GO:0003824 GO:0004807 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005975 GO:0005996 GO:0006006 GO:0006066 GO:0006071 GO:0006081 GO:0006082 GO:0006090 GO:0006091 GO:0006094 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006642 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009657 GO:0009658 GO:0009941 GO:0009987 GO:0016043 GO:0016051 GO:0016052 GO:0016053 GO:0016310 GO:0016853 GO:0016860 GO:0016861 GO:0017144 GO:0018130 GO:0019318 GO:0019319 GO:0019359 GO:0019362 GO:0019363 GO:0019400 GO:0019405 GO:0019438 GO:0019439 GO:0019563 GO:0019637 GO:0019682 GO:0019693 GO:0019751 GO:0019752 GO:0022622 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044262 GO:0044270 GO:0044271 GO:0044275 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046164 GO:0046166 GO:0046174 GO:0046184 GO:0046364 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046486 GO:0046496 GO:0046700 GO:0046939 GO:0048046 GO:0048364 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0071840 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0080022 GO:0090407 GO:0099402 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901616
KEGG Pathways
Metabolic & Signaling
ko00010 ko00051 ko00562 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00051 map00562 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
TIM

Protein Analysis

319

Amino Acids

33.79

Weight (kDa)

6.54

Isoelectric Point (pI)

34.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIM PF00121 70 - 310 4.6e-92 Triosephosphate isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 5, 160
AclWI GGATC 2 cut(s) 342, 826
AcoI YGGCCR 2 cut(s) 189, 714
AcsI RAATTY 2 cut(s) 382, 908
AcuI CTGAAG 1 cut(s) 650
AfaI GTAC 2 cut(s) 651, 741
AflIII ACRYGT 2 cut(s) 487, 742
AgsI TTSAA 3 cut(s) 439, 541, 898
AluBI AGCT 6 cut(s) 259, 348, 527, 657, 731, 847
AluI AGCT 6 cut(s) 259, 348, 527, 657, 731, 847
Alw26I GTCTC 2 cut(s) 108, 478
AlwI GGATC 2 cut(s) 342, 826
AlwNI CAGNNNCTG 1 cut(s) 647
AoxI GGCC 7 cut(s) 9, 40, 70, 165, 189, 693, 714
ApeKI GCWGC 3 cut(s) 450, 527, 950
ApoI RAATTY 2 cut(s) 382, 908
AspLEI GCGC 1 cut(s) 281
AspS9I GGNCC 3 cut(s) 41, 693, 902
AsuHPI GGTGA 3 cut(s) 352, 509, 584
AvaII GGWCC 1 cut(s) 902
BaeGI GKGCMC 1 cut(s) 474
BalI TGGCCA 1 cut(s) 716
BbvCI CCTCAGC 1 cut(s) 140
BbvI GCAGC 3 cut(s) 437, 514, 937
BccI CCATC 2 cut(s) 88, 863
BceAI ACGGC 1 cut(s) 161
BcgI CGANNNNNNTGC 2 cut(s) 66, 100
BclI TGATCA 1 cut(s) 505
BcoDI GTCTC 2 cut(s) 108, 478
BfaI CTAG 3 cut(s) 260, 654, 878
BisI GCNGC 3 cut(s) 451, 528, 951
BlpI GCTNAGC 1 cut(s) 843
BlsI GCNGC 3 cut(s) 452, 529, 952
Bme18I GGWCC 1 cut(s) 902
BmgT120I GGNCC 3 cut(s) 41, 693, 902
BmiI GGNNCC 3 cut(s) 196, 291, 903
BmsI GCATC 2 cut(s) 634, 800
BpmI CTGGAG 1 cut(s) 154
Bpu10I CCTNAGC 2 cut(s) 140, 732
Bpu1102I GCTNAGC 1 cut(s) 843
BsaJI CCNNGG 1 cut(s) 186
BsaWI WCCGGW 2 cut(s) 197, 374
BsaXI ACNNNNNCTCC 2 cut(s) 807, 837
Bse1I ACTGG 4 cut(s) 100, 227, 709, 717
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 2 cut(s) 80, 935
BseMII CTCAG 3 cut(s) 154, 746, 834
BseNI ACTGG 4 cut(s) 100, 227, 709, 717
BseRI GAGGAG 4 cut(s) 112, 139, 142, 145
BseSI GKGCMC 1 cut(s) 474
BseXI GCAGC 3 cut(s) 437, 514, 937
BseYI CCCAGC 1 cut(s) 657
BshFI GGCC 7 cut(s) 11, 42, 72, 167, 191, 695, 716
BsiSI CCGG 5 cut(s) 39, 69, 192, 198, 375
BslFI GGGAC 1 cut(s) 915
BsmAI GTCTC 2 cut(s) 108, 478
BsmFI GGGAC 1 cut(s) 915
BsnI GGCC 7 cut(s) 11, 42, 72, 167, 191, 695, 716
Bsp1286I GDGCHC 1 cut(s) 474
Bsp143I GATC 5 cut(s) 249, 334, 358, 505, 818
Bsp1720I GCTNAGC 1 cut(s) 843
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 2 cut(s) 5, 160
BspANI GGCC 7 cut(s) 11, 42, 72, 167, 191, 695, 716
BspCNI CTCAG 3 cut(s) 153, 745, 835
BspLI GGNNCC 3 cut(s) 196, 291, 903
BspPI GGATC 2 cut(s) 342, 826
BsrI ACTGG 4 cut(s) 100, 227, 709, 717
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 5 cut(s) 249, 334, 358, 505, 818
BssT1I CCWWGG 1 cut(s) 186
Bst6I CTCTTC 1 cut(s) 134
BstC8I GCNNGC 3 cut(s) 257, 565, 849
BstDEI CTNAG 3 cut(s) 140, 732, 843
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 2 cut(s) 80, 935
BstHHI GCGC 1 cut(s) 281
BstKTI GATC 5 cut(s) 252, 337, 361, 508, 821
BstMAI GTCTC 2 cut(s) 108, 478
BstMBI GATC 5 cut(s) 249, 334, 358, 505, 818
BstMWI GCNNNNNNNGC 5 cut(s) 173, 182, 533, 788, 848
BstNSI RCATGY 2 cut(s) 491, 746
BstSLI GKGCMC 1 cut(s) 474
BstV1I GCAGC 3 cut(s) 437, 514, 937
BstX2I RGATCY 1 cut(s) 818
BstYI RGATCY 1 cut(s) 818
BsuRI GGCC 7 cut(s) 11, 42, 72, 167, 191, 695, 716
BtgI CCRYGG 1 cut(s) 186
BtsCI GGATG 2 cut(s) 80, 935
BtsIMutI CAGTG 1 cut(s) 430
Cac8I GCNNGC 3 cut(s) 257, 565, 849
CaiI CAGNNNCTG 1 cut(s) 647
CfoI GCGC 1 cut(s) 281
Cfr13I GGNCC 3 cut(s) 41, 693, 902
Csp6I GTAC 2 cut(s) 650, 740
CviAII CATG 3 cut(s) 187, 488, 743
CviQI GTAC 2 cut(s) 650, 740
DdeI CTNAG 3 cut(s) 140, 732, 843
DpnI GATC 5 cut(s) 251, 336, 360, 507, 820
DpnII GATC 5 cut(s) 249, 334, 358, 505, 818
EaeI YGGCCR 2 cut(s) 189, 714
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
Eco130I CCWWGG 1 cut(s) 186
Eco147I AGGCCT 1 cut(s) 167
Eco32I GATATC 1 cut(s) 372
Eco47I GGWCC 1 cut(s) 902
Eco57I CTGAAG 1 cut(s) 650
EcoO109I RGGNCCY 1 cut(s) 902
EcoRV GATATC 1 cut(s) 372
EcoT14I CCWWGG 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 3 cut(s) 190, 491, 746
FaqI GGGAC 1 cut(s) 915
FatI CATG 3 cut(s) 186, 487, 742
FauI CCCGC 1 cut(s) 167
FbaI TGATCA 1 cut(s) 505
Fnu4HI GCNGC 3 cut(s) 451, 528, 951
FokI GGATG 2 cut(s) 67, 922
Fsp4HI GCNGC 3 cut(s) 451, 528, 951
FspBI CTAG 3 cut(s) 260, 654, 878
GlaI GCGC 1 cut(s) 280
GluI GCNGC 3 cut(s) 451, 528, 951
GsaI CCCAGC 1 cut(s) 661
GsuI CTGGAG 1 cut(s) 154
HaeIII GGCC 7 cut(s) 11, 42, 72, 167, 191, 695, 716
HapII CCGG 5 cut(s) 39, 69, 192, 198, 375
HhaI GCGC 1 cut(s) 281
Hin1II CATG 3 cut(s) 190, 491, 746
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HinfI GANTC 1 cut(s) 245
HpaII CCGG 5 cut(s) 39, 69, 192, 198, 375
HphI GGTGA 3 cut(s) 352, 509, 584
Hpy166II GTNNAC 2 cut(s) 722, 826
Hpy188I TCNGA 4 cut(s) 77, 268, 395, 478
Hpy188III TCNNGA 3 cut(s) 171, 734, 755
Hpy8I GTNNAC 2 cut(s) 722, 826
Hpy99I CGWCG 2 cut(s) 182, 185
HpyAV CCTTC 3 cut(s) 433, 625, 892
HpyCH4IV ACGT 1 cut(s) 612
HpyCH4V TGCA 4 cut(s) 453, 567, 641, 851
HpyF10VI GCNNNNNNNGC 5 cut(s) 173, 182, 533, 788, 848
HpyF3I CTNAG 3 cut(s) 140, 732, 843
HpySE526I ACGT 1 cut(s) 612
Hsp92II CATG 3 cut(s) 190, 491, 746
HspAI GCGC 1 cut(s) 279
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 5 cut(s) 249, 334, 358, 505, 818
Lsp1109I GCAGC 3 cut(s) 437, 514, 937
LweI GCATC 2 cut(s) 634, 800
MaeI CTAG 3 cut(s) 260, 654, 878
MaeII ACGT 1 cut(s) 612
MaeIII GTNAC 1 cut(s) 931
MalI GATC 5 cut(s) 251, 336, 360, 507, 820
MboI GATC 5 cut(s) 249, 334, 358, 505, 818
MboII GAAGA 4 cut(s) 121, 512, 596, 872
MfeI CAATTG 1 cut(s) 434
MflI RGATCY 1 cut(s) 818
MhlI GDGCHC 1 cut(s) 474
MlsI TGGCCA 1 cut(s) 716
MluCI AATT 7 cut(s) 284, 382, 434, 492, 558, 804, 908
MluNI TGGCCA 1 cut(s) 716
MlyI GAGTC 1 cut(s) 239
MmeI TCCRAC 3 cut(s) 100, 157, 373
Mox20I TGGCCA 1 cut(s) 716
MscI TGGCCA 1 cut(s) 716
MseI TTAA 1 cut(s) 272
Msp20I TGGCCA 1 cut(s) 716
MspI CCGG 5 cut(s) 39, 69, 192, 198, 375
MunI CAATTG 1 cut(s) 434
MwoI GCNNNNNNNGC 5 cut(s) 173, 182, 533, 788, 848
NcoI CCATGG 1 cut(s) 186
NdeII GATC 5 cut(s) 249, 334, 358, 505, 818
NlaIII CATG 3 cut(s) 190, 491, 746
NlaIV GGNNCC 3 cut(s) 196, 291, 903
NspI RCATGY 2 cut(s) 491, 746
PceI AGGCCT 1 cut(s) 167
PciI ACATGT 2 cut(s) 487, 742
PkrI GCNGC 3 cut(s) 452, 529, 952
PleI GAGTC 1 cut(s) 239
PpsI GAGTC 1 cut(s) 239
PpuMI RGGWCCY 1 cut(s) 902
PscI ACATGT 2 cut(s) 487, 742
Psp5II RGGWCCY 1 cut(s) 902
PspFI CCCAGC 1 cut(s) 657
PspN4I GGNNCC 3 cut(s) 196, 291, 903
PspPI GGNCC 3 cut(s) 41, 693, 902
PspPPI RGGWCCY 1 cut(s) 902
PstNI CAGNNNCTG 1 cut(s) 647
PsuI RGATCY 1 cut(s) 818
RsaI GTAC 2 cut(s) 651, 741
RsaNI GTAC 2 cut(s) 650, 740
SaqAI TTAA 1 cut(s) 272
SatI GCNGC 3 cut(s) 451, 528, 951
Sau3AI GATC 5 cut(s) 249, 334, 358, 505, 818
Sau96I GGNCC 3 cut(s) 41, 693, 902
SchI GAGTC 1 cut(s) 239
SduI GDGCHC 1 cut(s) 474
SfaNI GCATC 2 cut(s) 634, 800
SinI GGWCC 1 cut(s) 902
Sse9I AATT 7 cut(s) 284, 382, 434, 492, 558, 804, 908
SseBI AGGCCT 1 cut(s) 167
SsiI CCGC 2 cut(s) 5, 160
SspMI CTAG 3 cut(s) 260, 654, 878
StuI AGGCCT 1 cut(s) 167
StyI CCWWGG 1 cut(s) 186
TaiI ACGT 1 cut(s) 615
TaqI TCGA 2 cut(s) 96, 248
TasI AATT 7 cut(s) 284, 382, 434, 492, 558, 804, 908
TatI WGTACW 1 cut(s) 739
Tru1I TTAA 1 cut(s) 272
Tru9I TTAA 1 cut(s) 272
TscAI CASTG 1 cut(s) 430
TseI GCWGC 3 cut(s) 450, 527, 950
TspGWI ACGGA 2 cut(s) 496, 843
TspRI CASTG 1 cut(s) 430
VpaK11BI GGWCC 1 cut(s) 902
XapI RAATTY 2 cut(s) 382, 908
XceI RCATGY 2 cut(s) 491, 746
XspI CTAG 3 cut(s) 260, 654, 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.