Rh6DG022700

Triosephosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0009314
Physical Location & Seq
Forward (+)
1963052 .. 1967227
4176 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG022700.1

Sequence Viewer

Length: 684 bp
ATGTGTCACTTGTACGGTATGTATATACATCATCTGTTTGTATCAAAACTTTATATAAGTGGTAGAATGTTTCTAACTGTTGATTTTCCTGTAAAATGTACAGATGTTGTTGTAGCACCACCATTTCTTTACTTGGATCAGGTGAAGAGCTCTCTAACAGATCGTATTGAGATATCTGGTCAAAATTCTTGGGTTGGAAAAGGTGGGGCTTTCACTGGGGAAATCAGTGTGGAACAATTGAAGGATATTGGCTGCAAATGGGTTATTCTTGGGCACTCGGAACGGAGACATGTAATTGGTGAAGATGATCAGTTTATAGGAAAGAAAGCTGCCTATGCCTTGAACGAGGGTCTGGGAGTAATTGCTTGCATTGGTGAGAAGTTAGAAGAAAGGGAAGCCGGGAAAACTTTTGACATCTGTTTCCAGCAACTGAAGGCTTTTGCAGATGCTGTACCTAGTTGGGAAAATATAGTTGTTGCTTATGAGCCTGTATGGGCCATTGGAACTGGTAAGGTGGCCAGTCCACAACAAGCTCAGGAAGTACATGTAGCAGTTCGTGATTGGCTCAAAAAGAATGTGTCGGCAGAAGTTGCATCTAAAACCAGAATTATTTATGGAGGTAGCTACTTTCTGCAACTTTTGTTTAATGGTGATCCATTTCTTACAAATTTCACCTCTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003674 GO:0003824 GO:0004807 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005975 GO:0005996 GO:0006006 GO:0006066 GO:0006071 GO:0006081 GO:0006082 GO:0006090 GO:0006091 GO:0006094 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006642 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009657 GO:0009658 GO:0009941 GO:0009987 GO:0016043 GO:0016051 GO:0016052 GO:0016053 GO:0016310 GO:0016853 GO:0016860 GO:0016861 GO:0017144 GO:0018130 GO:0019318 GO:0019319 GO:0019359 GO:0019362 GO:0019363 GO:0019400 GO:0019405 GO:0019438 GO:0019439 GO:0019563 GO:0019637 GO:0019682 GO:0019693 GO:0019751 GO:0019752 GO:0022622 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044262 GO:0044270 GO:0044271 GO:0044275 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046164 GO:0046166 GO:0046174 GO:0046184 GO:0046364 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046486 GO:0046496 GO:0046700 GO:0046939 GO:0048046 GO:0048364 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0071840 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0080022 GO:0090407 GO:0099402 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901616
KEGG Pathways
Metabolic & Signaling
ko00010 ko00051 ko00562 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00051 map00562 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
TIM

Protein Analysis

227

Amino Acids

25.26

Weight (kDa)

5.88

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIM PF00121 34 - 208 7.3e-68 Triosephosphate isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 144, 647
AcoI YGGCCR 1 cut(s) 516
AcsI RAATTY 2 cut(s) 184, 667
AcuI CTGAAG 1 cut(s) 452
AfaI GTAC 4 cut(s) 14, 100, 453, 543
AflIII ACRYGT 2 cut(s) 289, 544
AgsI TTSAA 2 cut(s) 241, 343
AluBI AGCT 4 cut(s) 150, 329, 533, 624
AluI AGCT 4 cut(s) 150, 329, 533, 624
Alw21I GWGCWC 1 cut(s) 152
Alw26I GTCTC 1 cut(s) 280
AlwI GGATC 2 cut(s) 144, 647
AlwNI CAGNNNCTG 2 cut(s) 430, 449
AoxI GGCC 2 cut(s) 495, 516
ApeKI GCWGC 2 cut(s) 252, 329
ApoI RAATTY 2 cut(s) 184, 667
AspS9I GGNCC 1 cut(s) 495
AsuC2I CCSGG 1 cut(s) 400
AsuHPI GGTGA 5 cut(s) 154, 311, 386, 662, 664
BaeGI GKGCMC 1 cut(s) 276
BalI TGGCCA 1 cut(s) 518
BanII GRGCYC 1 cut(s) 152
Bbv12I GWGCWC 1 cut(s) 152
BbvI GCAGC 2 cut(s) 239, 316
BclI TGATCA 1 cut(s) 307
BcnI CCSGG 1 cut(s) 400
BcoDI GTCTC 1 cut(s) 280
BfaI CTAG 1 cut(s) 456
BisI GCNGC 2 cut(s) 253, 330
BlsI GCNGC 2 cut(s) 254, 331
Bme1390I CCNGG 1 cut(s) 400
BmgT120I GGNCC 1 cut(s) 495
BmrFI CCNGG 1 cut(s) 400
BmrI ACTGGG 1 cut(s) 225
BmsI GCATC 2 cut(s) 436, 602
BmuI ACTGGG 1 cut(s) 225
Bpu10I CCTNAGC 1 cut(s) 534
BpuMI CCSGG 1 cut(s) 400
Bse1I ACTGG 3 cut(s) 220, 511, 519
BseMII CTCAG 1 cut(s) 548
BseNI ACTGG 3 cut(s) 220, 511, 519
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 2 cut(s) 239, 316
BshFI GGCC 2 cut(s) 497, 518
BsiHKAI GWGCWC 1 cut(s) 152
BsiSI CCGG 1 cut(s) 399
BsmAI GTCTC 1 cut(s) 280
BsnI GGCC 2 cut(s) 497, 518
Bsp1286I GDGCHC 2 cut(s) 152, 276
Bsp1407I TGTACA 1 cut(s) 98
Bsp143I GATC 4 cut(s) 136, 160, 307, 652
BspANI GGCC 2 cut(s) 497, 518
BspCNI CTCAG 1 cut(s) 547
BspPI GGATC 2 cut(s) 144, 647
BspQI GCTCTTC 1 cut(s) 140
BsrGI TGTACA 1 cut(s) 98
BsrI ACTGG 3 cut(s) 220, 511, 519
BssMI GATC 4 cut(s) 136, 160, 307, 652
Bst4CI ACNGT 2 cut(s) 17, 79
Bst6I CTCTTC 1 cut(s) 140
BstAPI GCANNNNNTGC 1 cut(s) 590
BstAUI TGTACA 1 cut(s) 98
BstC8I GCNNGC 1 cut(s) 367
BstDEI CTNAG 1 cut(s) 534
BstKTI GATC 4 cut(s) 139, 163, 310, 655
BstMAI GTCTC 1 cut(s) 280
BstMBI GATC 4 cut(s) 136, 160, 307, 652
BstMWI GCNNNNNNNGC 2 cut(s) 335, 590
BstNSI RCATGY 2 cut(s) 293, 548
BstSCI CCNGG 1 cut(s) 398
BstSLI GKGCMC 1 cut(s) 276
BstV1I GCAGC 2 cut(s) 239, 316
BsuRI GGCC 2 cut(s) 497, 518
BtsIMutI CAGTG 2 cut(s) 213, 232
Cac8I GCNNGC 1 cut(s) 367
CaiI CAGNNNCTG 2 cut(s) 430, 449
Cfr13I GGNCC 1 cut(s) 495
Csp6I GTAC 4 cut(s) 13, 99, 452, 542
CviAII CATG 2 cut(s) 290, 545
CviQI GTAC 4 cut(s) 13, 99, 452, 542
DdeI CTNAG 1 cut(s) 534
DpnI GATC 4 cut(s) 138, 162, 309, 654
DpnII GATC 4 cut(s) 136, 160, 307, 652
EaeI YGGCCR 1 cut(s) 516
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
Ecl136II GAGCTC 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 152
Eco32I GATATC 1 cut(s) 174
Eco53kI GAGCTC 1 cut(s) 150
Eco57I CTGAAG 1 cut(s) 452
EcoICRI GAGCTC 1 cut(s) 150
EcoRV GATATC 1 cut(s) 174
EcoT38I GRGCYC 1 cut(s) 152
FaeI CATG 2 cut(s) 293, 548
FatI CATG 2 cut(s) 289, 544
FbaI TGATCA 1 cut(s) 307
Fnu4HI GCNGC 2 cut(s) 253, 330
FriOI GRGCYC 1 cut(s) 152
Fsp4HI GCNGC 2 cut(s) 253, 330
FspBI CTAG 1 cut(s) 456
GluI GCNGC 2 cut(s) 253, 330
HaeIII GGCC 2 cut(s) 497, 518
HapII CCGG 1 cut(s) 399
Hin1II CATG 2 cut(s) 293, 548
HpaII CCGG 1 cut(s) 399
HphI GGTGA 5 cut(s) 154, 311, 386, 662, 664
Hpy166II GTNNAC 1 cut(s) 524
Hpy188I TCNGA 2 cut(s) 280, 679
Hpy188III TCNNGA 2 cut(s) 536, 557
Hpy8I GTNNAC 1 cut(s) 524
HpyAV CCTTC 2 cut(s) 235, 427
HpyCH4III ACNGT 2 cut(s) 17, 79
HpyCH4V TGCA 5 cut(s) 255, 369, 443, 593, 634
HpyF10VI GCNNNNNNNGC 2 cut(s) 335, 590
HpyF3I CTNAG 1 cut(s) 534
Hsp92II CATG 2 cut(s) 293, 548
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 4 cut(s) 136, 160, 307, 652
LguI GCTCTTC 1 cut(s) 140
Lsp1109I GCAGC 2 cut(s) 239, 316
LweI GCATC 2 cut(s) 436, 602
MaeI CTAG 1 cut(s) 456
MaeIII GTNAC 1 cut(s) 5
MalI GATC 4 cut(s) 138, 162, 309, 654
MboI GATC 4 cut(s) 136, 160, 307, 652
MboII GAAGA 3 cut(s) 157, 314, 398
MfeI CAATTG 1 cut(s) 236
MhlI GDGCHC 2 cut(s) 152, 276
MlsI TGGCCA 1 cut(s) 518
MluCI AATT 6 cut(s) 184, 236, 294, 360, 606, 667
MluNI TGGCCA 1 cut(s) 518
MmeI TCCRAC 1 cut(s) 175
MnlI CCTC 2 cut(s) 340, 611
Mox20I TGGCCA 1 cut(s) 518
MscI TGGCCA 1 cut(s) 518
MseI TTAA 1 cut(s) 645
Msp20I TGGCCA 1 cut(s) 518
MspI CCGG 1 cut(s) 399
MspR9I CCNGG 1 cut(s) 400
MunI CAATTG 1 cut(s) 236
MwoI GCNNNNNNNGC 2 cut(s) 335, 590
NciI CCSGG 1 cut(s) 400
NdeII GATC 4 cut(s) 136, 160, 307, 652
NlaIII CATG 2 cut(s) 293, 548
NmuCI GTSAC 1 cut(s) 5
NspI RCATGY 2 cut(s) 293, 548
PciI ACATGT 2 cut(s) 289, 544
PciSI GCTCTTC 1 cut(s) 140
PkrI GCNGC 2 cut(s) 254, 331
PscI ACATGT 2 cut(s) 289, 544
Psp124BI GAGCTC 1 cut(s) 152
PspPI GGNCC 1 cut(s) 495
PstNI CAGNNNCTG 2 cut(s) 430, 449
RsaI GTAC 4 cut(s) 14, 100, 453, 543
RsaNI GTAC 4 cut(s) 13, 99, 452, 542
SacI GAGCTC 1 cut(s) 152
SapI GCTCTTC 1 cut(s) 140
SaqAI TTAA 1 cut(s) 645
SatI GCNGC 2 cut(s) 253, 330
Sau3AI GATC 4 cut(s) 136, 160, 307, 652
Sau96I GGNCC 1 cut(s) 495
ScrFI CCNGG 1 cut(s) 400
SduI GDGCHC 2 cut(s) 152, 276
SfaNI GCATC 2 cut(s) 436, 602
Sse9I AATT 6 cut(s) 184, 236, 294, 360, 606, 667
SspMI CTAG 1 cut(s) 456
SstI GAGCTC 1 cut(s) 152
StyD4I CCNGG 1 cut(s) 398
TaaI ACNGT 2 cut(s) 17, 79
TasI AATT 6 cut(s) 184, 236, 294, 360, 606, 667
TatI WGTACW 2 cut(s) 98, 541
Tru1I TTAA 1 cut(s) 645
Tru9I TTAA 1 cut(s) 645
TscAI CASTG 2 cut(s) 220, 232
TseFI GTSAC 1 cut(s) 5
TseI GCWGC 2 cut(s) 252, 329
Tsp45I GTSAC 1 cut(s) 5
TspGWI ACGGA 1 cut(s) 298
TspRI CASTG 2 cut(s) 220, 232
XapI RAATTY 2 cut(s) 184, 667
XceI RCATGY 2 cut(s) 293, 548
XspI CTAG 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.