Rh6CG022700

Triosephosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0009314
Physical Location & Seq
Forward (+)
2043555 .. 2047587
4033 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG022700.1

Sequence Viewer

Length: 759 bp
ATGTGTCACTTGTACGGTATGTATATACATCATCTGTTTGTATCAAAACTTTATATAAGTGGTAGAATGTTTCTAACTGTTGATTTTCCTGTAAAATGTACAGATGTTGTTGTAGCACCACCATTTCTTTACTTGGATCAGGTGAAGAGCTCTCTAACAGATCGTATTGAGATATCTGGTCAAAATTCTTGGGTTGGAAAAGGTGGGGCTTTCACTGGGGAAATCAGTGTGGAACAATTGAAGGATATTGGCTGCAAATGGGTTATTCTTGGGCACTCGGAACGGAGACATGTAATTGGTGAAGATGATCAGTTTATAGGAAAGAAAGCTGCCTATGCCTTGAACGAGGGTCTGGGAGTAATTGCTTGCATTGGTGAGAAGTTAGAAGAAAGGGAAGCCGGGAAAACTTTTGACATCTGTTTCCAGCAACTGAAGGCTTTTGCAGATGCTGTACCTAGTTGGGAAAATATAGTTGTTGCTTATGAGCCTGTATGGGCCATTGGAACTGGTAAGGTGGCCAGTCCACAACAAGCTCAGGAAGTACATGTAGCAGTTCGTGATTGGCTCAAAAAGAATGTGTCGGCAGAAGTTGCATCTAAAACCAGAATTATTTATGGAGGATCTGTAAACGGAGGCAATTCTGCTGAGCTTGCAAAGGAGGAAGATATTGATGGGTTTCTAGTTGGTGGTGCTTCCTTGAAGGGTCCTGAATTTGCTACCATTGTTAATGCTGTAACATCCAAGAAAGTTGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003674 GO:0003824 GO:0004807 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005975 GO:0005996 GO:0006006 GO:0006066 GO:0006071 GO:0006081 GO:0006082 GO:0006090 GO:0006091 GO:0006094 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006642 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009657 GO:0009658 GO:0009941 GO:0009987 GO:0016043 GO:0016051 GO:0016052 GO:0016053 GO:0016310 GO:0016853 GO:0016860 GO:0016861 GO:0017144 GO:0018130 GO:0019318 GO:0019319 GO:0019359 GO:0019362 GO:0019363 GO:0019400 GO:0019405 GO:0019438 GO:0019439 GO:0019563 GO:0019637 GO:0019682 GO:0019693 GO:0019751 GO:0019752 GO:0022622 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044262 GO:0044270 GO:0044271 GO:0044275 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046164 GO:0046166 GO:0046174 GO:0046184 GO:0046364 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046486 GO:0046496 GO:0046700 GO:0046939 GO:0048046 GO:0048364 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0071840 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0080022 GO:0090407 GO:0099402 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901616
KEGG Pathways
Metabolic & Signaling
ko00010 ko00051 ko00562 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00051 map00562 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
TIM

Protein Analysis

252

Amino Acids

27.38

Weight (kDa)

5.9

Isoelectric Point (pI)

33.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIM PF00121 34 - 244 1.1e-83 Triosephosphate isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 144, 628
AcoI YGGCCR 1 cut(s) 516
AcsI RAATTY 2 cut(s) 184, 710
AcuI CTGAAG 1 cut(s) 452
AfaI GTAC 4 cut(s) 14, 100, 453, 543
AflIII ACRYGT 2 cut(s) 289, 544
AgsI TTSAA 3 cut(s) 241, 343, 700
AluBI AGCT 4 cut(s) 150, 329, 533, 649
AluI AGCT 4 cut(s) 150, 329, 533, 649
Alw21I GWGCWC 1 cut(s) 152
Alw26I GTCTC 1 cut(s) 280
AlwI GGATC 2 cut(s) 144, 628
AlwNI CAGNNNCTG 2 cut(s) 430, 449
AoxI GGCC 2 cut(s) 495, 516
ApeKI GCWGC 3 cut(s) 252, 329, 752
ApoI RAATTY 2 cut(s) 184, 710
AspS9I GGNCC 2 cut(s) 495, 704
AsuC2I CCSGG 1 cut(s) 400
AsuHPI GGTGA 3 cut(s) 154, 311, 386
AvaII GGWCC 1 cut(s) 704
BaeGI GKGCMC 1 cut(s) 276
BalI TGGCCA 1 cut(s) 518
BanII GRGCYC 1 cut(s) 152
Bbv12I GWGCWC 1 cut(s) 152
BbvI GCAGC 3 cut(s) 239, 316, 739
BccI CCATC 1 cut(s) 665
BclI TGATCA 1 cut(s) 307
BcnI CCSGG 1 cut(s) 400
BcoDI GTCTC 1 cut(s) 280
BfaI CTAG 2 cut(s) 456, 680
BisI GCNGC 3 cut(s) 253, 330, 753
BlpI GCTNAGC 1 cut(s) 645
BlsI GCNGC 3 cut(s) 254, 331, 754
Bme1390I CCNGG 1 cut(s) 400
Bme18I GGWCC 1 cut(s) 704
BmgT120I GGNCC 2 cut(s) 495, 704
BmiI GGNNCC 1 cut(s) 705
BmrFI CCNGG 1 cut(s) 400
BmrI ACTGGG 1 cut(s) 225
BmsI GCATC 2 cut(s) 436, 602
BmuI ACTGGG 1 cut(s) 225
Bpu10I CCTNAGC 1 cut(s) 534
Bpu1102I GCTNAGC 1 cut(s) 645
BpuMI CCSGG 1 cut(s) 400
BsaXI ACNNNNNCTCC 2 cut(s) 609, 639
Bse1I ACTGG 3 cut(s) 220, 511, 519
BseGI GGATG 1 cut(s) 737
BseMII CTCAG 2 cut(s) 548, 636
BseNI ACTGG 3 cut(s) 220, 511, 519
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 3 cut(s) 239, 316, 739
BshFI GGCC 2 cut(s) 497, 518
BsiHKAI GWGCWC 1 cut(s) 152
BsiSI CCGG 1 cut(s) 399
BsmAI GTCTC 1 cut(s) 280
BsnI GGCC 2 cut(s) 497, 518
Bsp1286I GDGCHC 2 cut(s) 152, 276
Bsp1407I TGTACA 1 cut(s) 98
Bsp143I GATC 4 cut(s) 136, 160, 307, 620
Bsp1720I GCTNAGC 1 cut(s) 645
BspANI GGCC 2 cut(s) 497, 518
BspCNI CTCAG 2 cut(s) 547, 637
BspLI GGNNCC 1 cut(s) 705
BspPI GGATC 2 cut(s) 144, 628
BspQI GCTCTTC 1 cut(s) 140
BsrGI TGTACA 1 cut(s) 98
BsrI ACTGG 3 cut(s) 220, 511, 519
BssMI GATC 4 cut(s) 136, 160, 307, 620
Bst4CI ACNGT 2 cut(s) 17, 79
Bst6I CTCTTC 1 cut(s) 140
BstAPI GCANNNNNTGC 1 cut(s) 590
BstAUI TGTACA 1 cut(s) 98
BstC8I GCNNGC 2 cut(s) 367, 651
BstDEI CTNAG 2 cut(s) 534, 645
BstF5I GGATG 1 cut(s) 737
BstKTI GATC 4 cut(s) 139, 163, 310, 623
BstMAI GTCTC 1 cut(s) 280
BstMBI GATC 4 cut(s) 136, 160, 307, 620
BstMWI GCNNNNNNNGC 3 cut(s) 335, 590, 650
BstNSI RCATGY 2 cut(s) 293, 548
BstSCI CCNGG 1 cut(s) 398
BstSLI GKGCMC 1 cut(s) 276
BstV1I GCAGC 3 cut(s) 239, 316, 739
BstX2I RGATCY 1 cut(s) 620
BstYI RGATCY 1 cut(s) 620
BsuRI GGCC 2 cut(s) 497, 518
BtsCI GGATG 1 cut(s) 737
BtsIMutI CAGTG 2 cut(s) 213, 232
Cac8I GCNNGC 2 cut(s) 367, 651
CaiI CAGNNNCTG 2 cut(s) 430, 449
Cfr13I GGNCC 2 cut(s) 495, 704
Csp6I GTAC 4 cut(s) 13, 99, 452, 542
CviAII CATG 2 cut(s) 290, 545
CviQI GTAC 4 cut(s) 13, 99, 452, 542
DdeI CTNAG 2 cut(s) 534, 645
DpnI GATC 4 cut(s) 138, 162, 309, 622
DpnII GATC 4 cut(s) 136, 160, 307, 620
EaeI YGGCCR 1 cut(s) 516
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
Ecl136II GAGCTC 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 152
Eco32I GATATC 1 cut(s) 174
Eco47I GGWCC 1 cut(s) 704
Eco53kI GAGCTC 1 cut(s) 150
Eco57I CTGAAG 1 cut(s) 452
EcoICRI GAGCTC 1 cut(s) 150
EcoO109I RGGNCCY 1 cut(s) 704
EcoRV GATATC 1 cut(s) 174
EcoT38I GRGCYC 1 cut(s) 152
FaeI CATG 2 cut(s) 293, 548
FatI CATG 2 cut(s) 289, 544
FbaI TGATCA 1 cut(s) 307
Fnu4HI GCNGC 3 cut(s) 253, 330, 753
FokI GGATG 1 cut(s) 724
FriOI GRGCYC 1 cut(s) 152
Fsp4HI GCNGC 3 cut(s) 253, 330, 753
FspBI CTAG 2 cut(s) 456, 680
GluI GCNGC 3 cut(s) 253, 330, 753
HaeIII GGCC 2 cut(s) 497, 518
HapII CCGG 1 cut(s) 399
Hin1II CATG 2 cut(s) 293, 548
HpaII CCGG 1 cut(s) 399
HphI GGTGA 3 cut(s) 154, 311, 386
Hpy166II GTNNAC 2 cut(s) 524, 628
Hpy188I TCNGA 1 cut(s) 280
Hpy188III TCNNGA 3 cut(s) 536, 557, 707
Hpy8I GTNNAC 2 cut(s) 524, 628
HpyAV CCTTC 3 cut(s) 235, 427, 694
HpyCH4III ACNGT 2 cut(s) 17, 79
HpyCH4V TGCA 5 cut(s) 255, 369, 443, 593, 653
HpyF10VI GCNNNNNNNGC 3 cut(s) 335, 590, 650
HpyF3I CTNAG 2 cut(s) 534, 645
Hsp92II CATG 2 cut(s) 293, 548
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 4 cut(s) 136, 160, 307, 620
LguI GCTCTTC 1 cut(s) 140
Lsp1109I GCAGC 3 cut(s) 239, 316, 739
LweI GCATC 2 cut(s) 436, 602
MaeI CTAG 2 cut(s) 456, 680
MaeIII GTNAC 2 cut(s) 5, 733
MalI GATC 4 cut(s) 138, 162, 309, 622
MboI GATC 4 cut(s) 136, 160, 307, 620
MboII GAAGA 4 cut(s) 157, 314, 398, 674
MfeI CAATTG 1 cut(s) 236
MflI RGATCY 1 cut(s) 620
MhlI GDGCHC 2 cut(s) 152, 276
MlsI TGGCCA 1 cut(s) 518
MluCI AATT 7 cut(s) 184, 236, 294, 360, 606, 637, 710
MluNI TGGCCA 1 cut(s) 518
MmeI TCCRAC 1 cut(s) 175
MnlI CCTC 4 cut(s) 340, 611, 626, 652
Mox20I TGGCCA 1 cut(s) 518
MscI TGGCCA 1 cut(s) 518
MseI TTAA 1 cut(s) 726
Msp20I TGGCCA 1 cut(s) 518
MspI CCGG 1 cut(s) 399
MspR9I CCNGG 1 cut(s) 400
MunI CAATTG 1 cut(s) 236
MwoI GCNNNNNNNGC 3 cut(s) 335, 590, 650
NciI CCSGG 1 cut(s) 400
NdeII GATC 4 cut(s) 136, 160, 307, 620
NlaIII CATG 2 cut(s) 293, 548
NlaIV GGNNCC 1 cut(s) 705
NmuCI GTSAC 1 cut(s) 5
NspI RCATGY 2 cut(s) 293, 548
PciI ACATGT 2 cut(s) 289, 544
PciSI GCTCTTC 1 cut(s) 140
PkrI GCNGC 3 cut(s) 254, 331, 754
PpuMI RGGWCCY 1 cut(s) 704
PscI ACATGT 2 cut(s) 289, 544
Psp124BI GAGCTC 1 cut(s) 152
Psp5II RGGWCCY 1 cut(s) 704
PspN4I GGNNCC 1 cut(s) 705
PspPI GGNCC 2 cut(s) 495, 704
PspPPI RGGWCCY 1 cut(s) 704
PstNI CAGNNNCTG 2 cut(s) 430, 449
PsuI RGATCY 1 cut(s) 620
RsaI GTAC 4 cut(s) 14, 100, 453, 543
RsaNI GTAC 4 cut(s) 13, 99, 452, 542
SacI GAGCTC 1 cut(s) 152
SapI GCTCTTC 1 cut(s) 140
SaqAI TTAA 1 cut(s) 726
SatI GCNGC 3 cut(s) 253, 330, 753
Sau3AI GATC 4 cut(s) 136, 160, 307, 620
Sau96I GGNCC 2 cut(s) 495, 704
ScrFI CCNGG 1 cut(s) 400
SduI GDGCHC 2 cut(s) 152, 276
SetI ASST 8 cut(s) 144, 152, 205, 331, 457, 516, 535, 651
SfaNI GCATC 2 cut(s) 436, 602
SinI GGWCC 1 cut(s) 704
Sse9I AATT 7 cut(s) 184, 236, 294, 360, 606, 637, 710
SspMI CTAG 2 cut(s) 456, 680
SstI GAGCTC 1 cut(s) 152
StyD4I CCNGG 1 cut(s) 398
TaaI ACNGT 2 cut(s) 17, 79
TasI AATT 7 cut(s) 184, 236, 294, 360, 606, 637, 710
TatI WGTACW 2 cut(s) 98, 541
Tru1I TTAA 1 cut(s) 726
Tru9I TTAA 1 cut(s) 726
TscAI CASTG 2 cut(s) 220, 232
TseFI GTSAC 1 cut(s) 5
TseI GCWGC 3 cut(s) 252, 329, 752
Tsp45I GTSAC 1 cut(s) 5
TspGWI ACGGA 2 cut(s) 298, 645
TspRI CASTG 2 cut(s) 220, 232
VpaK11BI GGWCC 1 cut(s) 704
XapI RAATTY 2 cut(s) 184, 710
XceI RCATGY 2 cut(s) 293, 548
XspI CTAG 2 cut(s) 456, 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.