pycom05g11000

Triosephosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009314
Physical Location & Seq
Reverse (-)
14472165 .. 14475683
3519 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g11000.1

Sequence Viewer

Length: 1095 bp
ATGAAGTTAAAACCTAACCACCACAAGTGGCGAAGCTTCTCTCGCTCACTGACTCACTACCTCACTCGCACCAAACAAGTAAAACACCCCCAACTCGGAAGCTCCCAACTCACTGAGTTAGCAGCCATGGCGGTGGCATCCACATCTCTAGCCTCCCAACTCTCAGGCCCTAAATCCCTCTCCTCTTACTCCGGCCTCCGGCCGTCGTGCTCCAAGCTCGAATCCTCTCACTCTCTCTCCGCTACCCAATCCCTCTTCCAGCACCTCCACTCCCACCTCCGCCTCTCCTCCTCCTCCCGCAAGGCCTCCAGAGGCGTCGTCGCCATGGCCGGCACTGGAAAGTTCTTTGTTGGTGGAAACTGGAAGTGTAATGGCACAAAAGACTCCATCAGCAAGCTAGTTGCTGACTTGAACAGCGCAAAACTGGAACCGGATGTCGATGTTGTTGTAGCACCACCATTTCTTTACTTAGATCAGGTGAAGAGCTCGTTAACAGATCGTATTGAGTTATCTGGTCAAAATTCCTGGGTTGGAAAAGGTGGGGCTTTCACAGGAGAAATCAGTGTGGAACAATTGAAGGATATTGGAGCCACTTGGGTTATTCTTGGGCACTCAGAACGGAGACATGTGATTGGTGAAGATGACCAGTTTATAGGAAAGAAAGCTGCCTATGCGTTGAATGAGGGTCTTGGAGTAATTGCTTGCATTGGTGAGAAGCTAGAAGAAAGGGAAGCAGGGAAAACTTTTGACGTCTGCTTTCAGCAGCTGAAGGCTTTTGCAGACGCAGTACCCAGCTGGGATAAGATAGTTGTTGCTTATGAGCCTGTATGGGCCATTGGGACTGGTAAGGTAGCCAGTCCAGAACAAGCTCAGGAAGTACATGTAGCTGTTCGTGATTGGCTGAAACAGAATGTGTCAGCAGAAGTTGCATCTAAAACAAGAATTATTTATGGAGGATCTGTAAATGGAGGCAATTCTGCTGAGCTTGCAAAGAAGGAAGATATTGATGGTTTTCTTGTTGGCGGTGCTTCCCTAAAGGGCCCTGAATTTGCTACTATTGTCAACGCTGTGACAGCCAAGAAAGTTGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003674 GO:0003824 GO:0004807 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005975 GO:0005996 GO:0006006 GO:0006066 GO:0006071 GO:0006081 GO:0006082 GO:0006090 GO:0006091 GO:0006094 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006642 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009657 GO:0009658 GO:0009941 GO:0009987 GO:0016043 GO:0016051 GO:0016052 GO:0016053 GO:0016310 GO:0016853 GO:0016860 GO:0016861 GO:0017144 GO:0018130 GO:0019318 GO:0019319 GO:0019359 GO:0019362 GO:0019363 GO:0019400 GO:0019405 GO:0019438 GO:0019439 GO:0019563 GO:0019637 GO:0019682 GO:0019693 GO:0019751 GO:0019752 GO:0022622 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044262 GO:0044270 GO:0044271 GO:0044275 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046164 GO:0046166 GO:0046174 GO:0046184 GO:0046364 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046486 GO:0046496 GO:0046700 GO:0046939 GO:0048046 GO:0048364 GO:0048731 GO:0048856 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0071840 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0080022 GO:0090407 GO:0099402 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901616
KEGG Pathways
Metabolic & Signaling
ko00010 ko00051 ko00562 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00051 map00562 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
TIM

Protein Analysis

365

Amino Acids

39.03

Weight (kDa)

9.17

Isoelectric Point (pI)

33.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIM PF00121 116 - 356 5.7e-91 Triosephosphate isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 753
AciI CCGC 5 cut(s) 131, 240, 280, 298, 1023
AclWI GGATC 1 cut(s) 964
AcoI YGGCCR 2 cut(s) 200, 327
AcsI RAATTY 2 cut(s) 520, 1046
AcuI CTGAAG 1 cut(s) 788
AcyI GRCGYC 2 cut(s) 315, 750
AfaI GTAC 2 cut(s) 789, 879
AfiI CCNNNNNNNGG 2 cut(s) 95, 198
AflIII ACRYGT 2 cut(s) 625, 880
AgsI TTSAA 3 cut(s) 412, 577, 679
AjnI CCWGG 1 cut(s) 524
Alw21I GWGCWC 2 cut(s) 212, 488
Alw26I GTCTC 1 cut(s) 616
AlwI GGATC 1 cut(s) 964
AlwNI CAGNNNCTG 1 cut(s) 766
AoxI GGCC 7 cut(s) 166, 193, 200, 303, 327, 831, 1039
ApaI GGGCCC 1 cut(s) 1043
ApeKI GCWGC 4 cut(s) 122, 665, 763, 1088
ApoI RAATTY 2 cut(s) 520, 1046
AspLEI GCGC 1 cut(s) 419
AspS9I GGNCC 4 cut(s) 167, 831, 1039, 1040
AsuHPI GGTGA 3 cut(s) 490, 647, 722
BaeGI GKGCMC 2 cut(s) 612, 1043
BanII GRGCYC 2 cut(s) 488, 1043
Bbv12I GWGCWC 2 cut(s) 212, 488
BbvI GCAGC 4 cut(s) 134, 652, 775, 1075
BccI CCATC 2 cut(s) 395, 1001
BceAI ACGGC 1 cut(s) 187
BciT130I CCWGG 1 cut(s) 526
BcoDI GTCTC 1 cut(s) 616
BfaI CTAG 3 cut(s) 149, 398, 719
BisI GCNGC 4 cut(s) 123, 666, 764, 1089
BlpI GCTNAGC 1 cut(s) 981
BlsI GCNGC 4 cut(s) 124, 667, 765, 1090
Bme1390I CCNGG 1 cut(s) 526
BmgT120I GGNCC 4 cut(s) 167, 831, 1039, 1040
BmiI GGNNCC 3 cut(s) 429, 589, 1041
BmrFI CCNGG 1 cut(s) 526
BmsI GCATC 2 cut(s) 146, 938
BpmI CTGGAG 1 cut(s) 292
Bpu10I CCTNAGC 1 cut(s) 870
Bpu1102I GCTNAGC 1 cut(s) 981
BsaHI GRCGYC 2 cut(s) 315, 750
BsaJI CCNNGG 3 cut(s) 126, 324, 525
BsaWI WCCGGW 1 cut(s) 430
BsaXI ACNNNNNCTCC 6 cut(s) 221, 251, 254, 284, 945, 975
Bsc4I CCNNNNNNNGG 2 cut(s) 95, 198
Bse118I RCCGGY 1 cut(s) 329
Bse1I ACTGG 6 cut(s) 340, 365, 429, 646, 847, 855
BseBI CCWGG 1 cut(s) 526
BseDI CCNNGG 3 cut(s) 126, 324, 525
BseGI GGATG 2 cut(s) 137, 439
BseLI CCNNNNNNNGG 2 cut(s) 95, 198
BseMII CTCAG 5 cut(s) 105, 177, 627, 884, 972
BseNI ACTGG 6 cut(s) 340, 365, 429, 646, 847, 855
BseRI GAGGAG 4 cut(s) 172, 277, 280, 283
BseSI GKGCMC 2 cut(s) 612, 1043
BseX3I CGGCCG 1 cut(s) 200
BseXI GCAGC 4 cut(s) 134, 652, 775, 1075
BseYI CCCAGC 2 cut(s) 791, 795
Bsh1285I CGRYCG 1 cut(s) 203
BshFI GGCC 7 cut(s) 168, 195, 202, 305, 329, 833, 1041
BsiEI CGRYCG 1 cut(s) 203
BsiHKAI GWGCWC 2 cut(s) 212, 488
BsiSI CCGG 4 cut(s) 192, 199, 330, 431
BslFI GGGAC 1 cut(s) 853
BslI CCNNNNNNNGG 2 cut(s) 95, 198
BsmAI GTCTC 1 cut(s) 616
BsmFI GGGAC 1 cut(s) 853
BsnI GGCC 7 cut(s) 168, 195, 202, 305, 329, 833, 1041
Bsp120I GGGCCC 1 cut(s) 1039
Bsp1286I GDGCHC 4 cut(s) 212, 488, 612, 1043
Bsp143I GATC 3 cut(s) 472, 496, 956
Bsp1720I GCTNAGC 1 cut(s) 981
Bsp19I CCATGG 2 cut(s) 126, 324
BspACI CCGC 5 cut(s) 131, 240, 280, 298, 1023
BspANI GGCC 7 cut(s) 168, 195, 202, 305, 329, 833, 1041
BspCNI CTCAG 5 cut(s) 106, 176, 626, 883, 973
BspLI GGNNCC 3 cut(s) 429, 589, 1041
BspPI GGATC 1 cut(s) 964
BspQI GCTCTTC 1 cut(s) 476
BsrFI RCCGGY 1 cut(s) 329
BsrI ACTGG 6 cut(s) 340, 365, 429, 646, 847, 855
BssAI RCCGGY 1 cut(s) 329
BssECI CCNNGG 3 cut(s) 126, 324, 525
BssMI GATC 3 cut(s) 472, 496, 956
BssNI GRCGYC 2 cut(s) 315, 750
BssT1I CCWWGG 2 cut(s) 126, 324
Bst2UI CCWGG 1 cut(s) 526
Bst6I CTCTTC 2 cut(s) 260, 476
BstACI GRCGYC 2 cut(s) 315, 750
BstAPI GCANNNNNTGC 1 cut(s) 926
BstC8I GCNNGC 4 cut(s) 331, 395, 703, 987
BstDEI CTNAG 6 cut(s) 114, 163, 469, 613, 870, 981
BstDSI CCRYGG 2 cut(s) 126, 324
BstF5I GGATG 2 cut(s) 137, 439
BstHHI GCGC 1 cut(s) 419
BstKTI GATC 3 cut(s) 475, 499, 959
BstMAI GTCTC 1 cut(s) 616
BstMBI GATC 3 cut(s) 472, 496, 956
BstMCI CGRYCG 1 cut(s) 203
BstMWI GCNNNNNNNGC 6 cut(s) 42, 128, 671, 926, 986, 1073
BstNI CCWGG 1 cut(s) 526
BstNSI RCATGY 2 cut(s) 629, 884
BstSCI CCNGG 1 cut(s) 524
BstSLI GKGCMC 2 cut(s) 612, 1043
BstV1I GCAGC 4 cut(s) 134, 652, 775, 1075
BstX2I RGATCY 1 cut(s) 956
BstXI CCANNNNNNTGG 1 cut(s) 133
BstYI RGATCY 1 cut(s) 956
BstZI CGGCCG 1 cut(s) 200
BsuRI GGCC 7 cut(s) 168, 195, 202, 305, 329, 833, 1041
BtgI CCRYGG 2 cut(s) 126, 324
BtsCI GGATG 2 cut(s) 137, 439
BtsIMutI CAGTG 4 cut(s) 47, 111, 333, 568
Cac8I GCNNGC 4 cut(s) 331, 395, 703, 987
CaiI CAGNNNCTG 1 cut(s) 766
CfoI GCGC 1 cut(s) 419
Cfr10I RCCGGY 1 cut(s) 329
Cfr13I GGNCC 4 cut(s) 167, 831, 1039, 1040
CseI GACGC 2 cut(s) 304, 791
Csp6I GTAC 2 cut(s) 788, 878
CviAII CATG 4 cut(s) 127, 325, 626, 881
CviQI GTAC 2 cut(s) 788, 878
DdeI CTNAG 6 cut(s) 114, 163, 469, 613, 870, 981
DpnI GATC 3 cut(s) 474, 498, 958
DpnII GATC 3 cut(s) 472, 496, 956
EaeI YGGCCR 2 cut(s) 200, 327
EagI CGGCCG 1 cut(s) 200
Eam1104I CTCTTC 2 cut(s) 260, 476
EarI CTCTTC 2 cut(s) 260, 476
EciI GGCGGA 1 cut(s) 269
Ecl136II GAGCTC 1 cut(s) 486
EclXI CGGCCG 1 cut(s) 200
Eco130I CCWWGG 2 cut(s) 126, 324
Eco147I AGGCCT 1 cut(s) 305
Eco24I GRGCYC 2 cut(s) 488, 1043
Eco52I CGGCCG 1 cut(s) 200
Eco53kI GAGCTC 1 cut(s) 486
Eco57I CTGAAG 1 cut(s) 788
EcoICRI GAGCTC 1 cut(s) 486
EcoO109I RGGNCCY 3 cut(s) 167, 1039, 1040
EcoRII CCWGG 1 cut(s) 524
EcoT14I CCWWGG 2 cut(s) 126, 324
EcoT38I GRGCYC 2 cut(s) 488, 1043
ErhI CCWWGG 2 cut(s) 126, 324
FaeI CATG 4 cut(s) 130, 328, 629, 884
FaiI YATR 9 cut(s) 128, 326, 627, 653, 672, 819, 829, 882, 951
FaqI GGGAC 1 cut(s) 853
FatI CATG 4 cut(s) 126, 324, 625, 880
FauI CCCGC 1 cut(s) 305
Fnu4HI GCNGC 4 cut(s) 123, 666, 764, 1089
FokI GGATG 2 cut(s) 124, 446
FriOI GRGCYC 2 cut(s) 488, 1043
Fsp4HI GCNGC 4 cut(s) 123, 666, 764, 1089
FspBI CTAG 3 cut(s) 149, 398, 719
GlaI GCGC 1 cut(s) 418
GluI GCNGC 4 cut(s) 123, 666, 764, 1089
GsaI CCCAGC 2 cut(s) 795, 799
GsuI CTGGAG 1 cut(s) 292
HaeIII GGCC 7 cut(s) 168, 195, 202, 305, 329, 833, 1041
HapII CCGG 4 cut(s) 192, 199, 330, 431
HgaI GACGC 2 cut(s) 304, 791
HhaI GCGC 1 cut(s) 419
Hin1I GRCGYC 2 cut(s) 315, 750
Hin1II CATG 4 cut(s) 130, 328, 629, 884
Hin6I GCGC 1 cut(s) 417
HinP1I GCGC 1 cut(s) 417
HincII GTYRAC 2 cut(s) 492, 1063
HindII GTYRAC 2 cut(s) 492, 1063
HindIII AAGCTT 1 cut(s) 34
HinfI GANTC 3 cut(s) 52, 221, 383
HpaI GTTAAC 1 cut(s) 492
HpaII CCGG 4 cut(s) 192, 199, 330, 431
HphI GGTGA 3 cut(s) 490, 647, 722
Hpy166II GTNNAC 2 cut(s) 492, 1063
Hpy188I TCNGA 2 cut(s) 98, 616
Hpy188III TCNNGA 4 cut(s) 309, 860, 872, 893
Hpy8I GTNNAC 2 cut(s) 492, 1063
Hpy99I CGWCG 3 cut(s) 208, 320, 323
HpyAV CCTTC 3 cut(s) 571, 763, 988
HpyCH4IV ACGT 1 cut(s) 750
HpyCH4V TGCA 4 cut(s) 705, 779, 929, 989
HpyF10VI GCNNNNNNNGC 6 cut(s) 42, 128, 671, 926, 986, 1073
HpyF3I CTNAG 6 cut(s) 114, 163, 469, 613, 870, 981
HpySE526I ACGT 1 cut(s) 750
Hsp92I GRCGYC 2 cut(s) 315, 750
Hsp92II CATG 4 cut(s) 130, 328, 629, 884
HspAI GCGC 1 cut(s) 417
KroI GCCGGC 1 cut(s) 329
KroNI GCCGGC 1 cut(s) 331
KspAI GTTAAC 1 cut(s) 492
Kzo9I GATC 3 cut(s) 472, 496, 956
LguI GCTCTTC 1 cut(s) 476
LmnI GCTCC 3 cut(s) 107, 215, 587
Lsp1109I GCAGC 4 cut(s) 134, 652, 775, 1075
LweI GCATC 2 cut(s) 146, 938
MaeI CTAG 3 cut(s) 149, 398, 719
MaeII ACGT 1 cut(s) 750
MaeIII GTNAC 1 cut(s) 1069
MalI GATC 3 cut(s) 474, 498, 958
MboI GATC 3 cut(s) 472, 496, 956
MboII GAAGA 5 cut(s) 247, 493, 650, 734, 1010
MfeI CAATTG 1 cut(s) 572
MflI RGATCY 1 cut(s) 956
MhlI GDGCHC 4 cut(s) 212, 488, 612, 1043
MluCI AATT 6 cut(s) 520, 572, 696, 942, 973, 1046
MlyI GAGTC 2 cut(s) 46, 377
MmeI TCCRAC 1 cut(s) 511
MroNI GCCGGC 1 cut(s) 329
MseI TTAA 2 cut(s) 8, 491
MslI CAYNNNNRTG 1 cut(s) 131
MspA1I CMGCKG 2 cut(s) 766, 795
MspI CCGG 4 cut(s) 192, 199, 330, 431
MspR9I CCNGG 1 cut(s) 526
MunI CAATTG 1 cut(s) 572
MvaI CCWGG 1 cut(s) 526
MwoI GCNNNNNNNGC 6 cut(s) 42, 128, 671, 926, 986, 1073
NaeI GCCGGC 1 cut(s) 331
NcoI CCATGG 2 cut(s) 126, 324
NdeII GATC 3 cut(s) 472, 496, 956
NgoMIV GCCGGC 1 cut(s) 329
NlaIII CATG 4 cut(s) 130, 328, 629, 884
NlaIV GGNNCC 3 cut(s) 429, 589, 1041
NmuCI GTSAC 1 cut(s) 1069
NspI RCATGY 2 cut(s) 629, 884
PceI AGGCCT 1 cut(s) 305
PciI ACATGT 2 cut(s) 625, 880
PciSI GCTCTTC 1 cut(s) 476
PdiI GCCGGC 1 cut(s) 331
PfeI GAWTC 1 cut(s) 221
PkrI GCNGC 4 cut(s) 124, 667, 765, 1090
PleI GAGTC 2 cut(s) 46, 377
PpsI GAGTC 2 cut(s) 46, 377
PscI ACATGT 2 cut(s) 625, 880
Psp124BI GAGCTC 1 cut(s) 488
Psp6I CCWGG 1 cut(s) 524
PspFI CCCAGC 2 cut(s) 791, 795
PspGI CCWGG 1 cut(s) 524
PspN4I GGNNCC 3 cut(s) 429, 589, 1041
PspOMI GGGCCC 1 cut(s) 1039
PspPI GGNCC 4 cut(s) 167, 831, 1039, 1040
PstNI CAGNNNCTG 1 cut(s) 766
PsuI RGATCY 1 cut(s) 956
PvuII CAGCTG 2 cut(s) 766, 795
RsaI GTAC 2 cut(s) 789, 879
RsaNI GTAC 2 cut(s) 788, 878
RseI CAYNNNNRTG 1 cut(s) 131
SacI GAGCTC 1 cut(s) 488
SapI GCTCTTC 1 cut(s) 476
SaqAI TTAA 2 cut(s) 8, 491
SatI GCNGC 4 cut(s) 123, 666, 764, 1089
Sau3AI GATC 3 cut(s) 472, 496, 956
Sau96I GGNCC 4 cut(s) 167, 831, 1039, 1040
SchI GAGTC 2 cut(s) 46, 377
ScrFI CCNGG 1 cut(s) 526
SduI GDGCHC 4 cut(s) 212, 488, 612, 1043
SfaNI GCATC 2 cut(s) 146, 938
SmiMI CAYNNNNRTG 1 cut(s) 131
Sse9I AATT 6 cut(s) 520, 572, 696, 942, 973, 1046
SseBI AGGCCT 1 cut(s) 305
SsiI CCGC 5 cut(s) 131, 240, 280, 298, 1023
SspMI CTAG 3 cut(s) 149, 398, 719
SstI GAGCTC 1 cut(s) 488
StuI AGGCCT 1 cut(s) 305
StyD4I CCNGG 1 cut(s) 524
StyI CCWWGG 2 cut(s) 126, 324
TaiI ACGT 1 cut(s) 753
TaqI TCGA 2 cut(s) 219, 438
TasI AATT 6 cut(s) 520, 572, 696, 942, 973, 1046
TatI WGTACW 1 cut(s) 877
TfiI GAWTC 1 cut(s) 221
Tru1I TTAA 2 cut(s) 8, 491
Tru9I TTAA 2 cut(s) 8, 491
TscAI CASTG 4 cut(s) 54, 118, 340, 568
TseFI GTSAC 1 cut(s) 1069
TseI GCWGC 4 cut(s) 122, 665, 763, 1088
Tsp45I GTSAC 1 cut(s) 1069
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 634
TspRI CASTG 4 cut(s) 54, 118, 340, 568
XapI RAATTY 2 cut(s) 520, 1046
XceI RCATGY 2 cut(s) 629, 884
XspI CTAG 3 cut(s) 149, 398, 719
ZraI GACGTC 1 cut(s) 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.