AT2G21530

domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0013459
Physical Location & Seq
Forward (+)
9219252 .. 9220608
1357 bp
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UTR
Exon/CDS
Intron
AT2G21530.1

Sequence Viewer

Length: 630 bp
ATGGAGATAACAATATCCCAATTGTGCTCTCGAGTTAAGTTTTCATCATCATCATCATCATCATCACTTTTCTGTTTCTATGGAAACAGTAATAGAAACAACCTAAATGGAGCTGTTTCAGTAAACTGCTTGAGAGAGAATGAGCAGATCAGTAATATTAGCTTCATTGCGAAGAAACCAAGAAGTCATGCACCGGTGGTGAGAACAACACGCGCCTCCTTGGATGAGAATCAATCCCCAACTTCCGGAGGAGAACGGTGGCTTCTCAAACCAGTTGGCGATGGAGACACAAGACACATTGGTTACAAAGTGGCAATGCCTGCTCCTTTTGAGATCTCCTCTGGCCAAGTTACCATCGGACGGCTACCGGAAAAGGCTGACGTCGTGATTCCTGTTGCCACCGTGTCAGGAGTCCATGCGACAATCAATACGAATGAAAAGAACCTTCTTGTGACGGATATGAACAGCACCAACGGTACATTCATCGAAGACAAACGTCTAATTCCTGGTGTTGCTGCTCCTGCCTTTCCCGGAACACGAATCACCTTTGGAGATACAAATCTAGCAATTTTCCGTGTTTTCAAGCTCCAAGATAGTGAAGAATCCATAGAGAAACCAACTACAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FHA

Protein Analysis

209

Amino Acids

22.68

Weight (kDa)

7.73

Isoelectric Point (pI)

55.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yop-YscD_cpl PF16697 110 - 190 5e-08 Inner membrane component of T3SS, cytoplasmic domain
FHA PF00498 117 - 184 2.3e-14 FHA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013459)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21530
fragaria_vesca FvH4_3g27180 FvH4_3g27180 FvH4_3g27180
malus_domestica MD11G1181300.v1.1
prunus_persica Prupe.6G154100_v2.0.a1
pyrus_communis pycom11g15640
rosa_chinensis RchiOBHm_Chr5g0049851
rosa_laevigata RLG00000034657
rosa_multiflora Rmu_sc0001991.1_g000006
rosa_roxburghii Rroxscaffold_1G00031920
rosa_rugosa Rorug05G0253000 Rorug05G0253100
rosa_samantha Rh5AG328200 Rh5BG338700 Rh5CG363900 Rh5DG351100
rosa_wichuraiana Rw5G030990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 384
AccII CGCG 1 cut(s) 213
AccIII TCCGGA 1 cut(s) 245
AcoI YGGCCR 1 cut(s) 343
AcyI GRCGYC 1 cut(s) 381
AfaI GTAC 1 cut(s) 478
AfiI CCNNNNNNNGG 2 cut(s) 245, 360
AgeI ACCGGT 1 cut(s) 193
AgsI TTSAA 1 cut(s) 583
AjnI CCWGG 1 cut(s) 505
AjuI GAANNNNNNNTTGG 2 cut(s) 464, 496
AluBI AGCT 3 cut(s) 113, 162, 586
AluI AGCT 3 cut(s) 113, 162, 586
Alw21I GWGCWC 1 cut(s) 29
Alw26I GTCTC 1 cut(s) 279
Ama87I CYCGRG 1 cut(s) 30
Aor13HI TCCGGA 1 cut(s) 245
AoxI GGCC 1 cut(s) 343
ApeKI GCWGC 1 cut(s) 515
AsiGI ACCGGT 1 cut(s) 193
AspLEI GCGC 1 cut(s) 215
AsuC2I CCSGG 1 cut(s) 531
AsuHPI GGTGA 2 cut(s) 211, 535
AvaI CYCGRG 1 cut(s) 30
BalI TGGCCA 1 cut(s) 345
BbsI GAAGAC 1 cut(s) 495
Bbv12I GWGCWC 1 cut(s) 29
BbvI GCAGC 1 cut(s) 502
BccI CCATC 2 cut(s) 275, 362
BceAI ACGGC 1 cut(s) 377
BciT130I CCWGG 1 cut(s) 507
BcnI CCSGG 1 cut(s) 531
BcoDI GTCTC 1 cut(s) 279
BfaI CTAG 1 cut(s) 563
BfmI CTRYAG 1 cut(s) 621
BglII AGATCT 1 cut(s) 333
BisI GCNGC 1 cut(s) 516
BlsI GCNGC 1 cut(s) 517
Bme1390I CCNGG 2 cut(s) 507, 531
BmeT110I CYCGRG 1 cut(s) 30
BmrFI CCNGG 2 cut(s) 507, 531
BoxI GACNNNNGTC 1 cut(s) 495
BpiI GAAGAC 1 cut(s) 495
BplI GAGNNNNNCTC 2 cut(s) 323, 355
BpuEI CTTGAG 1 cut(s) 151
BpuMI CCSGG 1 cut(s) 531
BsaBI GATNNNNATC 2 cut(s) 228, 558
BsaHI GRCGYC 1 cut(s) 381
BsaJI CCNNGG 1 cut(s) 219
BsaWI WCCGGW 3 cut(s) 193, 245, 367
Bsc4I CCNNNNNNNGG 2 cut(s) 245, 360
Bse118I RCCGGY 1 cut(s) 193
Bse1I ACTGG 1 cut(s) 272
Bse3DI GCAATG 2 cut(s) 165, 321
Bse8I GATNNNNATC 2 cut(s) 228, 558
BseAI TCCGGA 1 cut(s) 245
BseBI CCWGG 1 cut(s) 507
BseDI CCNNGG 1 cut(s) 219
BseGI GGATG 1 cut(s) 229
BseJI GATNNNNATC 2 cut(s) 228, 558
BseLI CCNNNNNNNGG 2 cut(s) 245, 360
BseMI GCAATG 2 cut(s) 165, 321
BseNI ACTGG 1 cut(s) 272
BseRI GAGGAG 2 cut(s) 264, 328
BseXI GCAGC 1 cut(s) 502
Bsh1236I CGCG 1 cut(s) 213
BshFI GGCC 1 cut(s) 345
BshTI ACCGGT 1 cut(s) 193
BsiHKAI GWGCWC 1 cut(s) 29
BsiHKCI CYCGRG 1 cut(s) 30
BsiSI CCGG 4 cut(s) 194, 246, 368, 531
BslI CCNNNNNNNGG 2 cut(s) 245, 360
BsmAI GTCTC 1 cut(s) 279
BsnI GGCC 1 cut(s) 345
BsoBI CYCGRG 1 cut(s) 30
Bsp1286I GDGCHC 1 cut(s) 29
Bsp13I TCCGGA 1 cut(s) 245
Bsp143I GATC 2 cut(s) 147, 333
BspANI GGCC 1 cut(s) 345
BspEI TCCGGA 1 cut(s) 245
BspFNI CGCG 1 cut(s) 213
BsrDI GCAATG 2 cut(s) 165, 321
BsrFI RCCGGY 1 cut(s) 193
BsrI ACTGG 1 cut(s) 272
BssAI RCCGGY 1 cut(s) 193
BssECI CCNNGG 1 cut(s) 219
BssMI GATC 2 cut(s) 147, 333
BssNI GRCGYC 1 cut(s) 381
BssT1I CCWWGG 1 cut(s) 219
Bst2UI CCWGG 1 cut(s) 507
Bst4CI ACNGT 4 cut(s) 89, 258, 403, 476
BstACI GRCGYC 1 cut(s) 381
BstAPI GCANNNNNTGC 1 cut(s) 320
BstC8I GCNNGC 1 cut(s) 321
BstF5I GGATG 1 cut(s) 229
BstFNI CGCG 1 cut(s) 213
BstHHI GCGC 1 cut(s) 215
BstKTI GATC 2 cut(s) 150, 336
BstMAI GTCTC 1 cut(s) 279
BstMBI GATC 2 cut(s) 147, 333
BstMWI GCNNNNNNNGC 2 cut(s) 320, 521
BstNI CCWGG 1 cut(s) 507
BstPAI GACNNNNGTC 1 cut(s) 495
BstSCI CCNGG 2 cut(s) 505, 529
BstSFI CTRYAG 1 cut(s) 621
BstUI CGCG 1 cut(s) 213
BstV1I GCAGC 1 cut(s) 502
BstV2I GAAGAC 1 cut(s) 495
BstX2I RGATCY 1 cut(s) 333
BstYI RGATCY 1 cut(s) 333
BsuRI GGCC 1 cut(s) 345
BtgZI GCGATG 1 cut(s) 294
BtsCI GGATG 1 cut(s) 229
Cac8I GCNNGC 1 cut(s) 321
CfoI GCGC 1 cut(s) 215
Cfr10I RCCGGY 1 cut(s) 193
Csp6I GTAC 1 cut(s) 477
CspAI ACCGGT 1 cut(s) 193
CviAII CATG 2 cut(s) 188, 416
CviJI RGCY 7 cut(s) 113, 162, 262, 345, 364, 377, 586
CviKI_1 RGCY 7 cut(s) 113, 162, 262, 345, 364, 377, 586
CviQI GTAC 1 cut(s) 477
DpnI GATC 2 cut(s) 149, 335
DpnII GATC 2 cut(s) 147, 333
EaeI YGGCCR 1 cut(s) 343
Eco130I CCWWGG 1 cut(s) 219
Eco88I CYCGRG 1 cut(s) 30
EcoRII CCWGG 1 cut(s) 505
EcoT14I CCWWGG 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 2 cut(s) 191, 419
FaiI YATR 5 cut(s) 81, 189, 417, 461, 608
FatI CATG 2 cut(s) 187, 415
Fnu4HI GCNGC 1 cut(s) 516
FokI GGATG 1 cut(s) 236
Fsp4HI GCNGC 1 cut(s) 516
FspBI CTAG 1 cut(s) 563
GlaI GCGC 1 cut(s) 214
GluI GCNGC 1 cut(s) 516
HaeIII GGCC 1 cut(s) 345
HapII CCGG 4 cut(s) 194, 246, 368, 531
HhaI GCGC 1 cut(s) 215
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 2 cut(s) 191, 419
Hin6I GCGC 1 cut(s) 213
HinP1I GCGC 1 cut(s) 213
HinfI GANTC 5 cut(s) 229, 388, 411, 540, 602
HpaII CCGG 4 cut(s) 194, 246, 368, 531
HphI GGTGA 2 cut(s) 211, 535
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 1 cut(s) 359
Hpy188III TCNNGA 4 cut(s) 30, 246, 385, 408
Hpy8I GTNNAC 1 cut(s) 124
Hpy99I CGWCG 1 cut(s) 386
HpyAV CCTTC 1 cut(s) 455
HpyCH4III ACNGT 4 cut(s) 89, 258, 403, 476
HpyCH4IV ACGT 2 cut(s) 381, 496
HpyCH4V TGCA 1 cut(s) 191
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 521
HpySE526I ACGT 2 cut(s) 381, 496
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 2 cut(s) 191, 419
HspAI GCGC 1 cut(s) 213
Kpn2I TCCGGA 1 cut(s) 245
Kzo9I GATC 2 cut(s) 147, 333
LmnI GCTCC 4 cut(s) 110, 328, 523, 591
Lsp1109I GCAGC 1 cut(s) 502
MaeI CTAG 1 cut(s) 563
MaeII ACGT 2 cut(s) 381, 496
MaeIII GTNAC 3 cut(s) 302, 349, 451
MalI GATC 2 cut(s) 149, 335
MboI GATC 2 cut(s) 147, 333
MboII GAAGA 3 cut(s) 184, 500, 611
MfeI CAATTG 1 cut(s) 20
MflI RGATCY 1 cut(s) 333
MhlI GDGCHC 1 cut(s) 29
MlsI TGGCCA 1 cut(s) 345
MluCI AATT 3 cut(s) 20, 501, 567
MluNI TGGCCA 1 cut(s) 345
MlyI GAGTC 1 cut(s) 420
MnlI CCTC 3 cut(s) 226, 242, 349
Mox20I TGGCCA 1 cut(s) 345
MroI TCCGGA 1 cut(s) 245
MscI TGGCCA 1 cut(s) 345
MseI TTAA 1 cut(s) 36
Msp20I TGGCCA 1 cut(s) 345
MspI CCGG 4 cut(s) 194, 246, 368, 531
MspR9I CCNGG 2 cut(s) 507, 531
MunI CAATTG 1 cut(s) 20
MvaI CCWGG 1 cut(s) 507
MvnI CGCG 1 cut(s) 213
MwoI GCNNNNNNNGC 2 cut(s) 320, 521
NciI CCSGG 1 cut(s) 531
NdeII GATC 2 cut(s) 147, 333
NlaIII CATG 2 cut(s) 191, 419
NmuCI GTSAC 1 cut(s) 451
PaeR7I CTCGAG 1 cut(s) 30
PfeI GAWTC 4 cut(s) 229, 388, 540, 602
PfoI TCCNGGA 1 cut(s) 529
PinAI ACCGGT 1 cut(s) 193
PkrI GCNGC 1 cut(s) 517
PleI GAGTC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 419
PshAI GACNNNNGTC 1 cut(s) 495
Psp6I CCWGG 1 cut(s) 505
PspGI CCWGG 1 cut(s) 505
PsuI RGATCY 1 cut(s) 333
RsaI GTAC 1 cut(s) 478
RsaNI GTAC 1 cut(s) 477
SaqAI TTAA 1 cut(s) 36
SatI GCNGC 1 cut(s) 516
Sau3AI GATC 2 cut(s) 147, 333
SchI GAGTC 1 cut(s) 420
ScrFI CCNGG 2 cut(s) 507, 531
SduI GDGCHC 1 cut(s) 29
SetI ASST 8 cut(s) 105, 115, 164, 384, 447, 499, 548, 588
SfcI CTRYAG 1 cut(s) 621
Sfr274I CTCGAG 1 cut(s) 30
SgrAI CRCCGGYG 1 cut(s) 193
SlaI CTCGAG 1 cut(s) 30
SmlI CTYRAG 2 cut(s) 30, 130
SmoI CTYRAG 2 cut(s) 30, 130
Sse9I AATT 3 cut(s) 20, 501, 567
SspI AATATT 1 cut(s) 157
SspMI CTAG 1 cut(s) 563
StyD4I CCNGG 2 cut(s) 505, 529
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 4 cut(s) 89, 258, 403, 476
TaiI ACGT 2 cut(s) 384, 499
TaqI TCGA 2 cut(s) 31, 486
TasI AATT 3 cut(s) 20, 501, 567
TfiI GAWTC 4 cut(s) 229, 388, 540, 602
Tru1I TTAA 1 cut(s) 36
Tru9I TTAA 1 cut(s) 36
TseFI GTSAC 1 cut(s) 451
TseI GCWGC 1 cut(s) 515
Tsp45I GTSAC 1 cut(s) 451
TspDTI ATGAA 5 cut(s) 33, 154, 450, 472, 476
TspGWI ACGGA 2 cut(s) 470, 563
XhoI CTCGAG 1 cut(s) 30
XspI CTAG 1 cut(s) 563
ZraI GACGTC 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.