MD11G1181300.v1.1

Inner membrane component of T3SS, cytoplasmic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0013459
Physical Location & Seq
Forward (+)
22260388 .. 22263134
2747 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1181300.v1.1.491

Sequence Viewer

Length: 672 bp
ATGGAAGTCAGTGCTCACTGCCACTGCTACAAGTCTCTTGTTCTTCCCAAGCTTCCTCCTCATGCAGCTCATGCTGCTCTGAGTCACGTTGTTGCTTTTCGGCAGCGCTCCTCCAGTATTAATAGTACAAGTCCACAGCTCCTCCGACTCCAACGCCAACGCCAACCGCTACTTTTTAGTATCAAACCAAAATCAAAAAACCACCGAACAATTTATGCCGCCTCCTCTTCCTCCTCGGTTCAGACTCCACCAGATAAATGGCTTCTGGAGCCCGTTGGCGATGGAGATTCAAGGCATATAGGTTTCAAGGTTCCGATGCCAAATGCATTTGAAATTTCTTCTAATGAGGTGACTGTTGGCCGCCTCCCGGAAAAAGCTGATATGGTGATTCCAGTTGCAACAGTATCTGGCGTGCATGCTCGCATCCAAAAGAAAGAAGGGAAGCTATTAGTAACAGATTTGGACAGCACAAATGGGACGTTCATTGACGACAAACGGTTGAGCGCAGGAGTAGTTGCCACAGTACCACCTGGGTCTTGTCTTACGTTTGGAGATGAGCACTTGGCTGTGTTTCGCGTCTCAAAACTGGAGAATGTGGAAGCTGCAAGCAATCCAGATCCTTCTCAAGATAAAGTAGAGACTGATATCCCAATTGAAAGTACAAGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FHA

Protein Analysis

224

Amino Acids

24.25

Weight (kDa)

8.78

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yop-YscD_cpl PF16697 109 - 190 1e-08 Inner membrane component of T3SS, cytoplasmic domain
FHA PF00498 117 - 184 5.4e-14 FHA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013459)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21530
fragaria_vesca FvH4_3g27180 FvH4_3g27180 FvH4_3g27180
malus_domestica MD11G1181300.v1.1
prunus_persica Prupe.6G154100_v2.0.a1
pyrus_communis pycom11g15640
rosa_chinensis RchiOBHm_Chr5g0049851
rosa_laevigata RLG00000034657
rosa_multiflora Rmu_sc0001991.1_g000006
rosa_roxburghii Rroxscaffold_1G00031920
rosa_rugosa Rorug05G0253000 Rorug05G0253100
rosa_samantha Rh5AG328200 Rh5BG338700 Rh5CG363900 Rh5DG351100
rosa_wichuraiana Rw5G030990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 576
AciI CCGC 3 cut(s) 167, 219, 361
AclWI GGATC 1 cut(s) 611
AcoI YGGCCR 1 cut(s) 358
AcsI RAATTY 1 cut(s) 333
AfaI GTAC 3 cut(s) 127, 525, 661
AfeI AGCGCT 1 cut(s) 107
AfiI CCNNNNNNNGG 1 cut(s) 367
AgsI TTSAA 4 cut(s) 291, 307, 332, 656
AjnI CCWGG 1 cut(s) 529
AluBI AGCT 6 cut(s) 52, 68, 139, 377, 445, 602
AluI AGCT 6 cut(s) 52, 68, 139, 377, 445, 602
Alw21I GWGCWC 2 cut(s) 16, 561
Alw26I GTCTC 3 cut(s) 39, 583, 632
AlwI GGATC 1 cut(s) 611
AlwNI CAGNNNCTG 1 cut(s) 407
Aor51HI AGCGCT 1 cut(s) 107
AoxI GGCC 1 cut(s) 358
ApeKI GCWGC 4 cut(s) 65, 74, 103, 602
ApoI RAATTY 1 cut(s) 333
AseI ATTAAT 1 cut(s) 120
AspLEI GCGC 2 cut(s) 108, 506
AsuC2I CCSGG 1 cut(s) 368
AsuHPI GGTGA 2 cut(s) 361, 397
BanII GRGCYC 1 cut(s) 273
Bbv12I GWGCWC 2 cut(s) 16, 561
BbvI GCAGC 4 cut(s) 61, 77, 115, 589
BccI CCATC 1 cut(s) 275
BciT130I CCWGG 1 cut(s) 531
BcnI CCSGG 1 cut(s) 368
BcoDI GTCTC 3 cut(s) 39, 583, 632
BfoI RGCGCY 1 cut(s) 109
BisI GCNGC 6 cut(s) 66, 75, 104, 219, 361, 603
BlsI GCNGC 6 cut(s) 67, 76, 105, 220, 362, 604
Bme1390I CCNGG 2 cut(s) 368, 531
BmiI GGNNCC 2 cut(s) 270, 312
BmrFI CCNGG 2 cut(s) 368, 531
BmsI GCATC 2 cut(s) 306, 432
BpmI CTGGAG 3 cut(s) 97, 287, 608
BpuEI CTTGAG 1 cut(s) 609
BpuMI CCSGG 1 cut(s) 368
BsaJI CCNNGG 2 cut(s) 234, 530
BsaXI ACNNNNNCTCC 2 cut(s) 126, 156
Bsc4I CCNNNNNNNGG 1 cut(s) 367
Bse1I ACTGG 3 cut(s) 114, 392, 591
BseBI CCWGG 1 cut(s) 531
BseDI CCNNGG 2 cut(s) 234, 530
BseGI GGATG 1 cut(s) 423
BseLI CCNNNNNNNGG 1 cut(s) 367
BseMII CTCAG 1 cut(s) 71
BseNI ACTGG 3 cut(s) 114, 392, 591
BseRI GAGGAG 5 cut(s) 48, 100, 131, 214, 223
BseXI GCAGC 4 cut(s) 61, 77, 115, 589
Bsh1236I CGCG 1 cut(s) 576
BshFI GGCC 1 cut(s) 360
BsiHKAI GWGCWC 2 cut(s) 16, 561
BsiSI CCGG 1 cut(s) 368
BslFI GGGAC 1 cut(s) 490
BslI CCNNNNNNNGG 1 cut(s) 367
BsmAI GTCTC 3 cut(s) 39, 583, 632
BsmBI CGTCTC 1 cut(s) 583
BsmFI GGGAC 1 cut(s) 490
BsnI GGCC 1 cut(s) 360
Bsp1286I GDGCHC 3 cut(s) 16, 273, 561
Bsp143I GATC 1 cut(s) 616
BspACI CCGC 3 cut(s) 167, 219, 361
BspANI GGCC 1 cut(s) 360
BspCNI CTCAG 1 cut(s) 72
BspFNI CGCG 1 cut(s) 576
BspLI GGNNCC 2 cut(s) 270, 312
BspPI GGATC 1 cut(s) 611
BsrI ACTGG 3 cut(s) 114, 392, 591
BssECI CCNNGG 2 cut(s) 234, 530
BssMI GATC 1 cut(s) 616
Bst2UI CCWGG 1 cut(s) 531
Bst4CI ACNGT 4 cut(s) 355, 403, 498, 523
Bst6I CTCTTC 1 cut(s) 232
BstAPI GCANNNNNTGC 1 cut(s) 71
BstC8I GCNNGC 4 cut(s) 413, 417, 421, 607
BstDEI CTNAG 1 cut(s) 80
BstF5I GGATG 1 cut(s) 423
BstFNI CGCG 1 cut(s) 576
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 2 cut(s) 108, 506
BstKTI GATC 1 cut(s) 619
BstMAI GTCTC 3 cut(s) 39, 583, 632
BstMBI GATC 1 cut(s) 616
BstMWI GCNNNNNNNGC 3 cut(s) 71, 74, 268
BstNI CCWGG 1 cut(s) 531
BstNSI RCATGY 1 cut(s) 419
BstSCI CCNGG 2 cut(s) 366, 529
BstUI CGCG 1 cut(s) 576
BstV1I GCAGC 4 cut(s) 61, 77, 115, 589
BstX2I RGATCY 1 cut(s) 616
BstXI CCANNNNNNTGG 1 cut(s) 258
BstYI RGATCY 1 cut(s) 616
BsuRI GGCC 1 cut(s) 360
BtgZI GCGATG 1 cut(s) 294
BtsCI GGATG 1 cut(s) 423
BtsI GCAGTG 2 cut(s) 16, 22
BtsIMutI CAGTG 3 cut(s) 16, 16, 22
Cac8I GCNNGC 4 cut(s) 413, 417, 421, 607
CaiI CAGNNNCTG 1 cut(s) 407
CfoI GCGC 2 cut(s) 108, 506
CseI GACGC 1 cut(s) 565
Csp6I GTAC 3 cut(s) 126, 524, 660
CviAII CATG 3 cut(s) 62, 71, 416
CviQI GTAC 3 cut(s) 126, 524, 660
DdeI CTNAG 1 cut(s) 80
DpnI GATC 1 cut(s) 618
DpnII GATC 1 cut(s) 616
EaeI YGGCCR 1 cut(s) 358
Eam1104I CTCTTC 1 cut(s) 232
EarI CTCTTC 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 273
Eco32I GATATC 1 cut(s) 646
Eco47III AGCGCT 1 cut(s) 107
EcoRII CCWGG 1 cut(s) 529
EcoRV GATATC 1 cut(s) 646
EcoT22I ATGCAT 1 cut(s) 328
EcoT38I GRGCYC 1 cut(s) 273
Esp3I CGTCTC 1 cut(s) 583
FaeI CATG 3 cut(s) 65, 74, 419
FaiI YATR 7 cut(s) 63, 72, 216, 297, 299, 383, 417
FaqI GGGAC 1 cut(s) 490
FatI CATG 3 cut(s) 61, 70, 415
Fnu4HI GCNGC 6 cut(s) 66, 75, 104, 219, 361, 603
FokI GGATG 1 cut(s) 410
FriOI GRGCYC 1 cut(s) 273
Fsp4HI GCNGC 6 cut(s) 66, 75, 104, 219, 361, 603
GlaI GCGC 2 cut(s) 107, 505
GluI GCNGC 6 cut(s) 66, 75, 104, 219, 361, 603
GsuI CTGGAG 3 cut(s) 97, 287, 608
HaeII RGCGCY 1 cut(s) 109
HaeIII GGCC 1 cut(s) 360
HapII CCGG 1 cut(s) 368
HgaI GACGC 1 cut(s) 565
HhaI GCGC 2 cut(s) 108, 506
Hin1II CATG 3 cut(s) 65, 74, 419
Hin6I GCGC 2 cut(s) 106, 504
HinP1I GCGC 2 cut(s) 106, 504
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 5 cut(s) 82, 147, 244, 287, 388
HpaII CCGG 1 cut(s) 368
HphI GGTGA 2 cut(s) 361, 397
Hpy166II GTNNAC 1 cut(s) 134
Hpy188I TCNGA 4 cut(s) 81, 146, 243, 315
Hpy188III TCNNGA 3 cut(s) 266, 614, 626
Hpy8I GTNNAC 1 cut(s) 134
HpyAV CCTTC 2 cut(s) 431, 630
HpyCH4III ACNGT 4 cut(s) 355, 403, 498, 523
HpyCH4IV ACGT 3 cut(s) 87, 479, 545
HpyCH4V TGCA 5 cut(s) 65, 326, 398, 415, 605
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 74, 268
HpyF3I CTNAG 1 cut(s) 80
HpySE526I ACGT 3 cut(s) 87, 479, 545
Hsp92II CATG 3 cut(s) 65, 74, 419
HspAI GCGC 2 cut(s) 106, 504
Kzo9I GATC 1 cut(s) 616
LmnI GCTCC 3 cut(s) 113, 144, 268
Lsp1109I GCAGC 4 cut(s) 61, 77, 115, 589
LweI GCATC 2 cut(s) 306, 432
MaeII ACGT 3 cut(s) 87, 479, 545
MaeIII GTNAC 3 cut(s) 83, 349, 451
MalI GATC 1 cut(s) 618
MboI GATC 1 cut(s) 616
MboII GAAGA 3 cut(s) 35, 219, 330
MfeI CAATTG 1 cut(s) 651
MflI RGATCY 1 cut(s) 616
MhlI GDGCHC 3 cut(s) 16, 273, 561
MluCI AATT 3 cut(s) 210, 333, 651
MlyI GAGTC 3 cut(s) 91, 141, 238
MmeI TCCRAC 2 cut(s) 169, 175
Mph1103I ATGCAT 1 cut(s) 328
MseI TTAA 1 cut(s) 120
MspI CCGG 1 cut(s) 368
MspR9I CCNGG 2 cut(s) 368, 531
MunI CAATTG 1 cut(s) 651
MvaI CCWGG 1 cut(s) 531
MvnI CGCG 1 cut(s) 576
MwoI GCNNNNNNNGC 3 cut(s) 71, 74, 268
NciI CCSGG 1 cut(s) 368
NdeII GATC 1 cut(s) 616
NlaIII CATG 3 cut(s) 65, 74, 419
NlaIV GGNNCC 2 cut(s) 270, 312
NmuCI GTSAC 2 cut(s) 83, 349
NsiI ATGCAT 1 cut(s) 328
NspI RCATGY 1 cut(s) 419
PaeI GCATGC 1 cut(s) 419
PfeI GAWTC 2 cut(s) 287, 388
PfoI TCCNGGA 1 cut(s) 366
PkrI GCNGC 6 cut(s) 67, 76, 105, 220, 362, 604
PleI GAGTC 3 cut(s) 90, 141, 238
PpsI GAGTC 3 cut(s) 90, 141, 238
PshBI ATTAAT 1 cut(s) 120
Psp6I CCWGG 1 cut(s) 529
PspGI CCWGG 1 cut(s) 529
PspN4I GGNNCC 2 cut(s) 270, 312
PstNI CAGNNNCTG 1 cut(s) 407
PsuI RGATCY 1 cut(s) 616
RsaI GTAC 3 cut(s) 127, 525, 661
RsaNI GTAC 3 cut(s) 126, 524, 660
SaqAI TTAA 1 cut(s) 120
SatI GCNGC 6 cut(s) 66, 75, 104, 219, 361, 603
Sau3AI GATC 1 cut(s) 616
SchI GAGTC 3 cut(s) 91, 141, 238
ScrFI CCNGG 2 cut(s) 368, 531
SduI GDGCHC 3 cut(s) 16, 273, 561
SfaNI GCATC 2 cut(s) 306, 432
SmlI CTYRAG 1 cut(s) 624
SmoI CTYRAG 1 cut(s) 624
SphI GCATGC 1 cut(s) 419
Sse9I AATT 3 cut(s) 210, 333, 651
SsiI CCGC 3 cut(s) 167, 219, 361
StyD4I CCNGG 2 cut(s) 366, 529
TaaI ACNGT 4 cut(s) 355, 403, 498, 523
TaiI ACGT 3 cut(s) 90, 482, 548
TasI AATT 3 cut(s) 210, 333, 651
TatI WGTACW 2 cut(s) 125, 659
TauI GCSGC 2 cut(s) 221, 363
TfiI GAWTC 2 cut(s) 287, 388
Tru1I TTAA 1 cut(s) 120
Tru9I TTAA 1 cut(s) 120
TscAI CASTG 3 cut(s) 16, 23, 29
TseFI GTSAC 2 cut(s) 83, 349
TseI GCWGC 4 cut(s) 65, 74, 103, 602
Tsp45I GTSAC 2 cut(s) 83, 349
TspDTI ATGAA 1 cut(s) 472
TspRI CASTG 3 cut(s) 16, 23, 29
VspI ATTAAT 1 cut(s) 120
XapI RAATTY 1 cut(s) 333
XceI RCATGY 1 cut(s) 419
XcmI CCANNNNNNNNNTGG 1 cut(s) 255
Zsp2I ATGCAT 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.