AT2G22120

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0009975
Physical Location & Seq
Forward (+)
9393791 .. 9397098
3308 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G22120.1

Sequence Viewer

Length: 975 bp
ATGGCGGACGAACTGGAATTGTCGCCGTTGGTTCCACCGTCGCCGATGGTCGAGCCTTCCGAGATCGATCTCGAAGCTGGAGGACCTGGTGAACAAATTCAGTGCCGAATCTGCCTTGAAACTGATGGTAGGGATTTCATTGCCCCATGCAAATGCAAAGGAACATCAAAGTATGTACATCGTGATTGCTTGGATCATTGGAGAGCTATAAAGGAAGGTTTCGCGTTTGCGCACTGCACAACTTGCAAGGCTCCATATTATCTGAGAGTTCACAGTGCTGGAGATAGGAAATGGCGGACATTGAAGTTTCGCTTCTTTGTTACTCGAGACATTTTATCCATATTTCTTGCTGTTCAGCTTGTGATTGCTGCATTGGCCTACATGGTCTATTTCATAGATAGTTACCAGCAATCATGGCTTCGTCATATATGGGGTTTTGACAGTGAGGTCACGTTTTATTACATGTGTGGAGCTTTATTATTCTTTGCTCTGCTGGGTCTATCTGGATGCGTCATAACCTGCTATGATCGAAGGGTTCGCAATGACTTAGCACAGCCTTGCCGTGAATTGTGTCTCTGTTGTTGCCAGCCTGGGATATGCACAGACTGCCATCTACCAGGAACTATTTGTATGTGGGCAGATTGCACCGCATGTACTGAAGGCTGTGCAAGTGCAGTTAGTGAGTGTGGAGGTTGCCTTGGAGGTGCAGGGGAAGCCGGATTGCCATTACTCTTCATAACGGCATTAGTAATTCTCGGTCTATTTACAGTTATCGGTATATTCTATAGCGTATTGGTTGCAACCATGGTTGGACAGCGCATTTGGCAGCGCCATTACCACATTCTCGCCAAAAGAATGCTAACAAAGGAGTATGTGGTCGAGGATGTAGATGGCGAAATGACGGGGTCAGAATGGTCTCCCCCGGCTTTACCAACTGAGCATGTCCAGCAGCTGAAGACGCTTGGCCTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

324

Amino Acids

36.13

Weight (kDa)

5.72

Isoelectric Point (pI)

53.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 35 - 82 8.3e-11 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 446
Acc16I TGCGCA 1 cut(s) 231
Acc36I ACCTGC 1 cut(s) 527
AccII CGCG 1 cut(s) 224
AciI CCGC 3 cut(s) 5, 295, 648
AclWI GGATC 1 cut(s) 201
AcsI RAATTY 1 cut(s) 96
AcuI CTGAAG 2 cut(s) 678, 974
AfaI GTAC 2 cut(s) 177, 655
AflIII ACRYGT 1 cut(s) 462
AgsI TTSAA 2 cut(s) 119, 304
AjnI CCWGG 3 cut(s) 85, 589, 616
AluBI AGCT 5 cut(s) 77, 206, 358, 473, 952
AluI AGCT 5 cut(s) 77, 206, 358, 473, 952
Alw26I GTCTC 3 cut(s) 321, 578, 921
AlwI GGATC 1 cut(s) 201
AlwNI CAGNNNCTG 1 cut(s) 952
Ama87I CYCGRG 1 cut(s) 324
AoxI GGCC 2 cut(s) 375, 964
ApeKI GCWGC 3 cut(s) 368, 826, 949
ApoI RAATTY 1 cut(s) 96
ArsI GACNNNNNNTTYG 2 cut(s) 804, 836
Asp700I GAANNNNTTC 1 cut(s) 96
AspLEI GCGC 3 cut(s) 232, 819, 831
AspS9I GGNCC 1 cut(s) 83
AsuC2I CCSGG 1 cut(s) 923
AsuHPI GGTGA 1 cut(s) 101
AvaI CYCGRG 1 cut(s) 324
AvaII GGWCC 1 cut(s) 83
BbsI GAAGAC 1 cut(s) 962
BbvI GCAGC 3 cut(s) 355, 838, 961
BccI CCATC 4 cut(s) 40, 119, 618, 884
BceAI ACGGC 3 cut(s) 10, 546, 756
BciT130I CCWGG 3 cut(s) 87, 591, 618
BcnI CCSGG 1 cut(s) 923
BcoDI GTCTC 3 cut(s) 321, 578, 921
BfmI CTRYAG 1 cut(s) 784
BfoI RGCGCY 1 cut(s) 832
BfuAI ACCTGC 1 cut(s) 527
BisI GCNGC 3 cut(s) 369, 827, 950
BlsI GCNGC 3 cut(s) 370, 828, 951
Bme1390I CCNGG 4 cut(s) 87, 591, 618, 923
Bme18I GGWCC 1 cut(s) 83
BmeT110I CYCGRG 1 cut(s) 324
BmgT120I GGNCC 1 cut(s) 83
BmiI GGNNCC 2 cut(s) 33, 252
BmrFI CCNGG 4 cut(s) 87, 591, 618, 923
BmsI GCATC 1 cut(s) 497
BpiI GAAGAC 1 cut(s) 962
BpmI CTGGAG 2 cut(s) 99, 300
BpuMI CCSGG 1 cut(s) 923
Bsa29I ATCGAT 1 cut(s) 66
BsaI GGTCTC 1 cut(s) 921
BsaJI CCNNGG 4 cut(s) 590, 697, 804, 921
Bse1I ACTGG 1 cut(s) 18
Bse3DI GCAATG 2 cut(s) 138, 547
BseBI CCWGG 3 cut(s) 87, 591, 618
BseCI ATCGAT 1 cut(s) 66
BseDI CCNNGG 4 cut(s) 590, 697, 804, 921
BseGI GGATG 2 cut(s) 512, 889
BseMI GCAATG 2 cut(s) 138, 547
BseMII CTCAG 2 cut(s) 254, 927
BseNI ACTGG 1 cut(s) 18
BseXI GCAGC 3 cut(s) 355, 838, 961
BseYI CCCAGC 1 cut(s) 493
BsgI GTGCAG 3 cut(s) 220, 693, 726
Bsh1236I CGCG 1 cut(s) 224
BshFI GGCC 2 cut(s) 377, 966
BshVI ATCGAT 1 cut(s) 66
BsiHKCI CYCGRG 1 cut(s) 324
BsiSI CCGG 2 cut(s) 717, 923
BsmAI GTCTC 3 cut(s) 321, 578, 921
BsmI GAATGC 1 cut(s) 861
BsnI GGCC 2 cut(s) 377, 966
Bso31I GGTCTC 1 cut(s) 921
BsoBI CYCGRG 1 cut(s) 324
Bsp1407I TGTACA 1 cut(s) 175
Bsp143I GATC 4 cut(s) 63, 67, 193, 526
Bsp19I CCATGG 1 cut(s) 804
BspACI CCGC 3 cut(s) 5, 295, 648
BspANI GGCC 2 cut(s) 377, 966
BspCNI CTCAG 2 cut(s) 255, 928
BspDI ATCGAT 1 cut(s) 66
BspFNI CGCG 1 cut(s) 224
BspLI GGNNCC 2 cut(s) 33, 252
BspMI ACCTGC 1 cut(s) 527
BspPI GGATC 1 cut(s) 201
BspTNI GGTCTC 1 cut(s) 921
BsrDI GCAATG 2 cut(s) 138, 547
BsrGI TGTACA 1 cut(s) 175
BsrI ACTGG 1 cut(s) 18
BssECI CCNNGG 4 cut(s) 590, 697, 804, 921
BssMI GATC 4 cut(s) 63, 67, 193, 526
BssT1I CCWWGG 2 cut(s) 697, 804
Bst2UI CCWGG 3 cut(s) 87, 591, 618
Bst4CI ACNGT 4 cut(s) 39, 275, 443, 769
Bst6I CTCTTC 1 cut(s) 737
BstAPI GCANNNNNTGC 2 cut(s) 243, 606
BstAUI TGTACA 1 cut(s) 175
BstC8I GCNNGC 1 cut(s) 587
BstDEI CTNAG 3 cut(s) 263, 547, 936
BstDSI CCRYGG 1 cut(s) 804
BstF5I GGATG 2 cut(s) 512, 889
BstFNI CGCG 1 cut(s) 224
BstH2I RGCGCY 1 cut(s) 832
BstHHI GCGC 3 cut(s) 232, 819, 831
BstKTI GATC 4 cut(s) 66, 70, 196, 529
BstMAI GTCTC 3 cut(s) 321, 578, 921
BstMBI GATC 4 cut(s) 63, 67, 193, 526
BstMWI GCNNNNNNNGC 9 cut(s) 111, 243, 374, 415, 606, 713, 823, 946, 958
BstNI CCWGG 3 cut(s) 87, 591, 618
BstNSI RCATGY 3 cut(s) 466, 654, 944
BstSCI CCNGG 4 cut(s) 85, 589, 616, 921
BstSFI CTRYAG 1 cut(s) 784
BstUI CGCG 1 cut(s) 224
BstV1I GCAGC 3 cut(s) 355, 838, 961
BstV2I GAAGAC 1 cut(s) 962
Bsu15I ATCGAT 1 cut(s) 66
BsuRI GGCC 2 cut(s) 377, 966
BsuTUI ATCGAT 1 cut(s) 66
BtgI CCRYGG 1 cut(s) 804
BtsCI GGATG 2 cut(s) 512, 889
BtsI GCAGTG 1 cut(s) 232
BtsIMutI CAGTG 4 cut(s) 107, 232, 280, 448
BveI ACCTGC 1 cut(s) 527
Cac8I GCNNGC 1 cut(s) 587
CaiI CAGNNNCTG 1 cut(s) 952
CfoI GCGC 3 cut(s) 232, 819, 831
Cfr13I GGNCC 1 cut(s) 83
ClaI ATCGAT 1 cut(s) 66
CseI GACGC 2 cut(s) 499, 967
CsiI ACCWGGT 1 cut(s) 85
Csp6I GTAC 2 cut(s) 176, 654
CviAII CATG 7 cut(s) 147, 382, 414, 463, 651, 805, 941
CviQI GTAC 2 cut(s) 176, 654
DdeI CTNAG 3 cut(s) 263, 547, 936
DpnI GATC 4 cut(s) 65, 69, 195, 528
DpnII GATC 4 cut(s) 63, 67, 193, 526
DrdI GACNNNNNNGTC 1 cut(s) 446
DseDI GACNNNNNNGTC 1 cut(s) 446
Eam1104I CTCTTC 1 cut(s) 737
EarI CTCTTC 1 cut(s) 737
EciI GGCGGA 2 cut(s) 20, 310
Eco130I CCWWGG 2 cut(s) 697, 804
Eco31I GGTCTC 1 cut(s) 921
Eco47I GGWCC 1 cut(s) 83
Eco57I CTGAAG 2 cut(s) 678, 974
Eco88I CYCGRG 1 cut(s) 324
EcoO109I RGGNCCY 1 cut(s) 83
EcoRII CCWGG 3 cut(s) 85, 589, 616
EcoT14I CCWWGG 2 cut(s) 697, 804
ErhI CCWWGG 2 cut(s) 697, 804
FaeI CATG 7 cut(s) 150, 385, 417, 466, 654, 808, 944
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FatI CATG 7 cut(s) 146, 381, 413, 462, 650, 804, 940
Fnu4HI GCNGC 3 cut(s) 369, 827, 950
FokI GGATG 2 cut(s) 519, 896
Fsp4HI GCNGC 3 cut(s) 369, 827, 950
FspI TGCGCA 1 cut(s) 231
GlaI GCGC 3 cut(s) 231, 818, 830
GluI GCNGC 3 cut(s) 369, 827, 950
GsaI CCCAGC 1 cut(s) 497
GsuI CTGGAG 2 cut(s) 99, 300
HaeII RGCGCY 1 cut(s) 832
HaeIII GGCC 2 cut(s) 377, 966
HapII CCGG 2 cut(s) 717, 923
HgaI GACGC 2 cut(s) 499, 967
HhaI GCGC 3 cut(s) 232, 819, 831
Hin1II CATG 7 cut(s) 150, 385, 417, 466, 654, 808, 944
Hin6I GCGC 3 cut(s) 230, 817, 829
HinP1I GCGC 3 cut(s) 230, 817, 829
HinfI GANTC 1 cut(s) 108
HpaII CCGG 2 cut(s) 717, 923
HphI GGTGA 1 cut(s) 101
Hpy166II GTNNAC 2 cut(s) 92, 271
Hpy188I TCNGA 3 cut(s) 61, 264, 910
Hpy188III TCNNGA 4 cut(s) 71, 182, 326, 504
Hpy8I GTNNAC 2 cut(s) 92, 271
Hpy99I CGWCG 1 cut(s) 43
HpyAV CCTTC 4 cut(s) 66, 209, 525, 653
HpyCH4III ACNGT 4 cut(s) 39, 275, 443, 769
HpyCH4IV ACGT 1 cut(s) 452
HpyF10VI GCNNNNNNNGC 9 cut(s) 111, 243, 374, 415, 606, 713, 823, 946, 958
HpyF3I CTNAG 3 cut(s) 263, 547, 936
HpySE526I ACGT 1 cut(s) 452
Hsp92II CATG 7 cut(s) 150, 385, 417, 466, 654, 808, 944
HspAI GCGC 3 cut(s) 230, 817, 829
Kzo9I GATC 4 cut(s) 63, 67, 193, 526
LmnI GCTCC 2 cut(s) 256, 470
Lsp1109I GCAGC 3 cut(s) 355, 838, 961
LweI GCATC 1 cut(s) 497
MabI ACCWGGT 1 cut(s) 85
MaeII ACGT 1 cut(s) 452
MaeIII GTNAC 3 cut(s) 319, 401, 448
MalI GATC 4 cut(s) 65, 69, 195, 528
MboI GATC 4 cut(s) 63, 67, 193, 526
MboII GAAGA 2 cut(s) 724, 967
MluCI AATT 4 cut(s) 17, 96, 566, 750
MmeI TCCRAC 1 cut(s) 790
MnlI CCTC 5 cut(s) 74, 439, 683, 695, 874
MroXI GAANNNNTTC 1 cut(s) 96
MslI CAYNNNNRTG 1 cut(s) 151
MspA1I CMGCKG 1 cut(s) 952
MspI CCGG 2 cut(s) 717, 923
MspR9I CCNGG 4 cut(s) 87, 591, 618, 923
Mva1269I GAATGC 1 cut(s) 861
MvaI CCWGG 3 cut(s) 87, 591, 618
MvnI CGCG 1 cut(s) 224
MwoI GCNNNNNNNGC 9 cut(s) 111, 243, 374, 415, 606, 713, 823, 946, 958
NciI CCSGG 1 cut(s) 923
NcoI CCATGG 1 cut(s) 804
NdeII GATC 4 cut(s) 63, 67, 193, 526
NlaIII CATG 7 cut(s) 150, 385, 417, 466, 654, 808, 944
NlaIV GGNNCC 2 cut(s) 33, 252
NmuCI GTSAC 1 cut(s) 448
NsbI TGCGCA 1 cut(s) 231
NspI RCATGY 3 cut(s) 466, 654, 944
PaeR7I CTCGAG 1 cut(s) 324
PciI ACATGT 1 cut(s) 462
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PctI GAATGC 1 cut(s) 861
PdmI GAANNNNTTC 1 cut(s) 96
PfeI GAWTC 1 cut(s) 108
PflFI GACNNNGTC 1 cut(s) 904
PkrI GCNGC 3 cut(s) 370, 828, 951
PpuMI RGGWCCY 1 cut(s) 83
PscI ACATGT 1 cut(s) 462
Psp5II RGGWCCY 1 cut(s) 83
Psp6I CCWGG 3 cut(s) 85, 589, 616
PspFI CCCAGC 1 cut(s) 493
PspGI CCWGG 3 cut(s) 85, 589, 616
PspN4I GGNNCC 2 cut(s) 33, 252
PspPI GGNCC 1 cut(s) 83
PspPPI RGGWCCY 1 cut(s) 83
PstNI CAGNNNCTG 1 cut(s) 952
PsyI GACNNNGTC 1 cut(s) 904
PvuII CAGCTG 1 cut(s) 952
RsaI GTAC 2 cut(s) 177, 655
RsaNI GTAC 2 cut(s) 176, 654
RseI CAYNNNNRTG 1 cut(s) 151
SatI GCNGC 3 cut(s) 369, 827, 950
Sau3AI GATC 4 cut(s) 63, 67, 193, 526
Sau96I GGNCC 1 cut(s) 83
ScrFI CCNGG 4 cut(s) 87, 591, 618, 923
SexAI ACCWGGT 1 cut(s) 85
SfaNI GCATC 1 cut(s) 497
SfcI CTRYAG 1 cut(s) 784
Sfr274I CTCGAG 1 cut(s) 324
SinI GGWCC 1 cut(s) 83
SlaI CTCGAG 1 cut(s) 324
SmiMI CAYNNNNRTG 1 cut(s) 151
SmlI CTYRAG 1 cut(s) 324
SmoI CTYRAG 1 cut(s) 324
Sse9I AATT 4 cut(s) 17, 96, 566, 750
SsiI CCGC 3 cut(s) 5, 295, 648
StyD4I CCNGG 4 cut(s) 85, 589, 616, 921
StyI CCWWGG 2 cut(s) 697, 804
TaaI ACNGT 4 cut(s) 39, 275, 443, 769
TaiI ACGT 1 cut(s) 455
TaqI TCGA 6 cut(s) 51, 66, 72, 325, 529, 879
TaqII GACCGA 1 cut(s) 746
TasI AATT 4 cut(s) 17, 96, 566, 750
TatI WGTACW 2 cut(s) 175, 653
TfiI GAWTC 1 cut(s) 108
TscAI CASTG 4 cut(s) 107, 239, 280, 448
TseFI GTSAC 1 cut(s) 448
TseI GCWGC 3 cut(s) 368, 826, 949
Tsp45I GTSAC 1 cut(s) 448
TspDTI ATGAA 3 cut(s) 127, 382, 724
TspRI CASTG 4 cut(s) 107, 239, 280, 448
Tth111I GACNNNGTC 1 cut(s) 904
VpaK11BI GGWCC 1 cut(s) 83
XapI RAATTY 1 cut(s) 96
XceI RCATGY 3 cut(s) 466, 654, 944
XhoI CTCGAG 1 cut(s) 324
XmnI GAANNNNTTC 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.