MD01G1021800.v1.1

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009975
Physical Location & Seq
Forward (+)
8674367 .. 8680244
5878 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1021800.v1.1.491

Sequence Viewer

Length: 969 bp
ATGGCCGATCACTCAGACGCTTCGCCTCTCATCCCTCCGAAGCAGATCACCGAGCCTAGCGAGATCGACCTCGAGGCTGGCCCTGGCGAACAAATCCAGTGCCGTATTTGCCTTGAAAGTGATGGCAGGGACTTCATTGCTCCGTGCAAGTGCAAGGGGACTTCCAAGTATGTCCACCGGGAGTGTTTGGATCATTGGAGAGCTATAAAGGAAGGGTTTGCATTTGCTCATTGTACAACCTGCAAGGCTCCTTATCACTTGAGAGTTCATGTTGTTGCTGACAGAAAATGGCGAACCTTGAAATTCCGATTCTTTGTGACGAGGGACATTATATTTATATTTCTGGCCGTTCAGCTTGTGATTACTTCATTGGCCTATTTGGCTTATTCAATAGATGGTTTCCAGCAATTCTGGCTTCATCTTGCATGGGGTTTTGACAGCAAGCTTAGTTTCTACTATATATGCGGAGCTTTGTTGTTTTTCGCTTTGCTTGGGTTGTCTGGCTGCTTCATTACTTGTTATGACCGTAGAGTGCGCAATGATTTGGCTCAGCCATGTCGTGAATTATGCCTTTGTTGCTGTCAGCCTGGTGTGTGCGCAGACTGTCATTTACCTGGAACACTTTGTATGTGGACTGACTGTACTACATGCTGGGGAAGCTGTGCAAGTATGGCAGGTGAATGTGGTTGCTTGGGAGGCGCTGGTGAAGCAGGGCTACCAATATTATTTATAATGGCTCTGATTGTACTGGGACTTTTTACCGTGATTGGGATATTCTACAGTGTCCTGGTAGCTACAATGGTTGGACAACGGATTTGGCAGCGGCATTATCATATACTTGCAAAAAGGATGCTGACAAAAGAATATGTTGTCGAGGATGTTGATGGTGAGATGACAGGATCTGATTGGTCTGCCCCTCCTCTCCCACCTGAGCATATTCAGCAGCTGAAAGCGCTCGGCCTATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

323

Amino Acids

36.06

Weight (kDa)

6.4

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 34 - 81 1.2e-10 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 731
AarI CACCTGC 1 cut(s) 665
AbsI CCTCGAGG 1 cut(s) 71
Acc16I TGCGCA 2 cut(s) 536, 598
Acc36I ACCTGC 2 cut(s) 248, 665
AciI CCGC 2 cut(s) 465, 823
AclWI GGATC 2 cut(s) 198, 907
AcoI YGGCCR 2 cut(s) 3, 345
AcsI RAATTY 1 cut(s) 302
AfaI GTAC 3 cut(s) 235, 643, 747
AfeI AGCGCT 1 cut(s) 954
AfiI CCNNNNNNNGG 1 cut(s) 768
AgsI TTSAA 3 cut(s) 116, 301, 390
AjnI CCWGG 4 cut(s) 82, 586, 613, 786
AluBI AGCT 7 cut(s) 203, 355, 445, 470, 660, 794, 946
AluI AGCT 7 cut(s) 203, 355, 445, 470, 660, 794, 946
AlwI GGATC 2 cut(s) 198, 907
AlwNI CAGNNNCTG 2 cut(s) 902, 946
Ama87I CYCGRG 1 cut(s) 71
Aor51HI AGCGCT 1 cut(s) 954
AoxI GGCC 5 cut(s) 3, 79, 345, 372, 958
ApeKI GCWGC 3 cut(s) 504, 820, 943
ApoI RAATTY 1 cut(s) 302
ArsI GACNNNNNNTTYG 2 cut(s) 798, 830
AspLEI GCGC 4 cut(s) 537, 599, 701, 955
AspS9I GGNCC 1 cut(s) 80
AsuC2I CCSGG 1 cut(s) 179
AsuHPI GGTGA 4 cut(s) 40, 689, 716, 899
AvaI CYCGRG 1 cut(s) 71
BarI GAAGNNNNNNTAC 2 cut(s) 699, 731
BbvI GCAGC 3 cut(s) 491, 832, 955
BccI CCATC 3 cut(s) 116, 389, 878
BceAI ACGGC 2 cut(s) 87, 332
BciT130I CCWGG 4 cut(s) 84, 588, 615, 788
BcnI CCSGG 1 cut(s) 179
BfaI CTAG 1 cut(s) 57
BfmI CTRYAG 1 cut(s) 778
BfoI RGCGCY 2 cut(s) 702, 956
BfuAI ACCTGC 2 cut(s) 248, 665
BglI GCCNNNNNGGC 1 cut(s) 380
BisI GCNGC 4 cut(s) 505, 821, 824, 944
BlpI GCTNAGC 1 cut(s) 549
BlsI GCNGC 4 cut(s) 506, 822, 825, 945
Bme1390I CCNGG 5 cut(s) 84, 179, 588, 615, 788
BmeT110I CYCGRG 1 cut(s) 71
BmgT120I GGNCC 1 cut(s) 80
BmiI GGNNCC 1 cut(s) 249
BmrFI CCNGG 5 cut(s) 84, 179, 588, 615, 788
BmrI ACTGGG 1 cut(s) 758
BmsI GCATC 1 cut(s) 840
BmuI ACTGGG 1 cut(s) 758
Bpu10I CCTNAGC 1 cut(s) 930
Bpu1102I GCTNAGC 1 cut(s) 549
BpuEI CTTGAG 1 cut(s) 280
BpuMI CCSGG 1 cut(s) 179
BsaJI CCNNGG 1 cut(s) 82
Bsc4I CCNNNNNNNGG 1 cut(s) 768
Bse1I ACTGG 2 cut(s) 97, 753
Bse3DI GCAATG 2 cut(s) 135, 544
BseBI CCWGG 4 cut(s) 84, 588, 615, 788
BseDI CCNNGG 1 cut(s) 82
BseGI GGATG 3 cut(s) 30, 855, 883
BseLI CCNNNNNNNGG 1 cut(s) 768
BseMI GCAATG 2 cut(s) 135, 544
BseMII CTCAG 3 cut(s) 27, 563, 921
BseNI ACTGG 2 cut(s) 97, 753
BseRI GAGGAG 1 cut(s) 909
BseXI GCAGC 3 cut(s) 491, 832, 955
BseYI CCCAGC 1 cut(s) 651
BshFI GGCC 5 cut(s) 5, 81, 347, 374, 960
BsiHKCI CYCGRG 1 cut(s) 71
BsiSI CCGG 1 cut(s) 178
BslFI GGGAC 4 cut(s) 143, 172, 338, 765
BslI CCNNNNNNNGG 1 cut(s) 768
BsmFI GGGAC 4 cut(s) 143, 172, 338, 765
BsnI GGCC 5 cut(s) 5, 81, 347, 374, 960
BsoBI CYCGRG 1 cut(s) 71
Bsp1407I TGTACA 1 cut(s) 233
Bsp143I GATC 5 cut(s) 7, 45, 63, 190, 899
Bsp1720I GCTNAGC 1 cut(s) 549
BspACI CCGC 2 cut(s) 465, 823
BspANI GGCC 5 cut(s) 5, 81, 347, 374, 960
BspCNI CTCAG 3 cut(s) 26, 562, 922
BspLI GGNNCC 1 cut(s) 249
BspMI ACCTGC 2 cut(s) 248, 665
BspPI GGATC 2 cut(s) 198, 907
BsrDI GCAATG 2 cut(s) 135, 544
BsrGI TGTACA 1 cut(s) 233
BsrI ACTGG 2 cut(s) 97, 753
BssECI CCNNGG 1 cut(s) 82
BssMI GATC 5 cut(s) 7, 45, 63, 190, 899
Bst2UI CCWGG 4 cut(s) 84, 588, 615, 788
Bst4CI ACNGT 5 cut(s) 527, 605, 641, 763, 782
BstAUI TGTACA 1 cut(s) 233
BstC8I GCNNGC 2 cut(s) 79, 443
BstDEI CTNAG 4 cut(s) 13, 446, 549, 930
BstF5I GGATG 3 cut(s) 30, 855, 883
BstH2I RGCGCY 2 cut(s) 702, 956
BstHHI GCGC 4 cut(s) 537, 599, 701, 955
BstKTI GATC 5 cut(s) 10, 48, 66, 193, 902
BstMBI GATC 5 cut(s) 7, 45, 63, 190, 899
BstNI CCWGG 4 cut(s) 84, 588, 615, 788
BstNSI RCATGY 1 cut(s) 651
BstSCI CCNGG 5 cut(s) 82, 177, 586, 613, 786
BstSFI CTRYAG 1 cut(s) 778
BstV1I GCAGC 3 cut(s) 491, 832, 955
BstX2I RGATCY 1 cut(s) 899
BstYI RGATCY 1 cut(s) 899
BsuRI GGCC 5 cut(s) 5, 81, 347, 374, 960
BtsCI GGATG 3 cut(s) 30, 855, 883
BtsIMutI CAGTG 2 cut(s) 104, 787
BveI ACCTGC 2 cut(s) 248, 665
Cac8I GCNNGC 2 cut(s) 79, 443
CaiI CAGNNNCTG 2 cut(s) 902, 946
CfoI GCGC 4 cut(s) 537, 599, 701, 955
Cfr13I GGNCC 1 cut(s) 80
CseI GACGC 1 cut(s) 26
Csp6I GTAC 3 cut(s) 234, 642, 746
CviAII CATG 4 cut(s) 269, 426, 555, 648
CviQI GTAC 3 cut(s) 234, 642, 746
DdeI CTNAG 4 cut(s) 13, 446, 549, 930
DpnI GATC 5 cut(s) 9, 47, 65, 192, 901
DpnII GATC 5 cut(s) 7, 45, 63, 190, 899
EaeI YGGCCR 2 cut(s) 3, 345
Eco47III AGCGCT 1 cut(s) 954
Eco88I CYCGRG 1 cut(s) 71
EcoRII CCWGG 4 cut(s) 82, 586, 613, 786
FaeI CATG 4 cut(s) 272, 429, 558, 651
FaqI GGGAC 4 cut(s) 143, 172, 338, 765
FatI CATG 4 cut(s) 268, 425, 554, 647
Fnu4HI GCNGC 4 cut(s) 505, 821, 824, 944
FokI GGATG 3 cut(s) 17, 862, 890
Fsp4HI GCNGC 4 cut(s) 505, 821, 824, 944
FspBI CTAG 1 cut(s) 57
FspI TGCGCA 2 cut(s) 536, 598
GlaI GCGC 4 cut(s) 536, 598, 700, 954
GluI GCNGC 4 cut(s) 505, 821, 824, 944
GsaI CCCAGC 1 cut(s) 655
HaeII RGCGCY 2 cut(s) 702, 956
HaeIII GGCC 5 cut(s) 5, 81, 347, 374, 960
HapII CCGG 1 cut(s) 178
HgaI GACGC 1 cut(s) 26
HhaI GCGC 4 cut(s) 537, 599, 701, 955
Hin1II CATG 4 cut(s) 272, 429, 558, 651
Hin6I GCGC 4 cut(s) 535, 597, 699, 953
HinP1I GCGC 4 cut(s) 535, 597, 699, 953
HindIII AAGCTT 1 cut(s) 443
HinfI GANTC 1 cut(s) 309
HpaII CCGG 1 cut(s) 178
HphI GGTGA 4 cut(s) 40, 689, 716, 899
Hpy166II GTNNAC 2 cut(s) 175, 633
Hpy188I TCNGA 5 cut(s) 16, 39, 308, 741, 904
Hpy188III TCNNGA 1 cut(s) 560
Hpy8I GTNNAC 2 cut(s) 175, 633
HpyAV CCTTC 1 cut(s) 206
HpyCH4III ACNGT 5 cut(s) 527, 605, 641, 763, 782
HpyCH4V TGCA 7 cut(s) 147, 153, 221, 243, 425, 665, 842
HpyF3I CTNAG 4 cut(s) 13, 446, 549, 930
Hsp92II CATG 4 cut(s) 272, 429, 558, 651
HspAI GCGC 4 cut(s) 535, 597, 699, 953
Kzo9I GATC 5 cut(s) 7, 45, 63, 190, 899
LmnI GCTCC 3 cut(s) 145, 253, 467
Lsp1109I GCAGC 3 cut(s) 491, 832, 955
LweI GCATC 1 cut(s) 840
MaeI CTAG 1 cut(s) 57
MaeIII GTNAC 1 cut(s) 316
MalI GATC 5 cut(s) 9, 47, 65, 192, 901
MboI GATC 5 cut(s) 7, 45, 63, 190, 899
MflI RGATCY 1 cut(s) 899
MluCI AATT 3 cut(s) 302, 407, 563
MmeI TCCRAC 1 cut(s) 784
MnlI CCTC 9 cut(s) 36, 45, 67, 80, 315, 689, 868, 927, 930
MspA1I CMGCKG 2 cut(s) 823, 946
MspI CCGG 1 cut(s) 178
MspR9I CCNGG 5 cut(s) 84, 179, 588, 615, 788
MvaI CCWGG 4 cut(s) 84, 588, 615, 788
NciI CCSGG 1 cut(s) 179
NdeII GATC 5 cut(s) 7, 45, 63, 190, 899
NlaIII CATG 4 cut(s) 272, 429, 558, 651
NlaIV GGNNCC 1 cut(s) 249
NmeAIII GCCGAG 1 cut(s) 936
NmuCI GTSAC 1 cut(s) 316
NsbI TGCGCA 2 cut(s) 536, 598
NspI RCATGY 1 cut(s) 651
PaeR7I CTCGAG 1 cut(s) 71
PaqCI CACCTGC 1 cut(s) 665
PfeI GAWTC 1 cut(s) 309
PkrI GCNGC 4 cut(s) 506, 822, 825, 945
PsiI TTATAA 1 cut(s) 731
Psp6I CCWGG 4 cut(s) 82, 586, 613, 786
PspFI CCCAGC 1 cut(s) 651
PspGI CCWGG 4 cut(s) 82, 586, 613, 786
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 1 cut(s) 80
PspXI VCTCGAGB 1 cut(s) 71
PstNI CAGNNNCTG 2 cut(s) 902, 946
PsuI RGATCY 1 cut(s) 899
PvuII CAGCTG 1 cut(s) 946
RsaI GTAC 3 cut(s) 235, 643, 747
RsaNI GTAC 3 cut(s) 234, 642, 746
SatI GCNGC 4 cut(s) 505, 821, 824, 944
Sau3AI GATC 5 cut(s) 7, 45, 63, 190, 899
Sau96I GGNCC 1 cut(s) 80
ScrFI CCNGG 5 cut(s) 84, 179, 588, 615, 788
SfaNI GCATC 1 cut(s) 840
SfcI CTRYAG 1 cut(s) 778
Sfr274I CTCGAG 1 cut(s) 71
SlaI CTCGAG 1 cut(s) 71
SmlI CTYRAG 2 cut(s) 71, 259
SmoI CTYRAG 2 cut(s) 71, 259
Sse9I AATT 3 cut(s) 302, 407, 563
SsiI CCGC 2 cut(s) 465, 823
SspI AATATT 1 cut(s) 723
SspMI CTAG 1 cut(s) 57
StyD4I CCNGG 5 cut(s) 82, 177, 586, 613, 786
TaaI ACNGT 5 cut(s) 527, 605, 641, 763, 782
TaqI TCGA 3 cut(s) 66, 72, 873
TasI AATT 3 cut(s) 302, 407, 563
TatI WGTACW 3 cut(s) 233, 641, 745
TauI GCSGC 1 cut(s) 826
TfiI GAWTC 1 cut(s) 309
TscAI CASTG 2 cut(s) 104, 787
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 3 cut(s) 504, 820, 943
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 5 cut(s) 124, 257, 357, 407, 499
TspGWI ACGGA 2 cut(s) 132, 826
TspRI CASTG 2 cut(s) 104, 787
XapI RAATTY 1 cut(s) 302
XceI RCATGY 1 cut(s) 651
XhoI CTCGAG 1 cut(s) 71
XspI CTAG 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.