FvH4_1g21840

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0009975
Physical Location & Seq
Reverse (-)
13756511 .. 13763612
7102 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g21840.t1

Sequence Viewer

Length: 969 bp
ATGGCGGATGACTCCGACGCTTCTCCGCTCATCGCTCCGACCCCGATCACCGAGCCCAGTGAGATCGACCTCGAAGCCGGCCCCGGCGAGCAAATCCAGTGCCGAATTTGCCTCGAAAGTGATGGTAGGGATTTTATTGCTCCTTGCAAATGCAAAGGGACTTCGAAGTATGTTCACAGGGAATGTTTGGATCATTGGAGAGCTGTAAAGGAAGGTTTTGCATTTGCTCATTGCACAACCTGCAAGGCTCCTTACCATTTGAGAGTTCATCAAGCTGCCGATAGGAAATGGCGAACCTTGAAATTTCGGTTCTTCGTGACCAGAGACATTATATTCATATTTCTCGCTGTCCAGCTTGTGATTGCTTCATTGGCATATTTGGTGTTTCTAATAGATGGTTTCCAGCAATTTTGGCTTCGTCTTGCATGGGGTTTTGATACGGGACCAAGTTTTTACTATATATGTGGAGCTTTGCTGTTCTTCGCGTTGCTTGGATTATCTGGGTGCTTCATTACTTGTTATGACCGAAGAGTGCGCAACGATTTGGCTCAGCCTTGTCGAGAGTTATGTCTTTGTTGCTGTCAGCCTGGTGTCTGCGCAGATTGTCATTTACCTGGCACTCTTTGTATGTGGACTGACTGTACCACATGCTGGGGAAGTTGTGCAAGTATGGCCGGAGAATGTGGTTGCTTGGGAGGTGCTGGTGAAGCAGGGCTTCCAATCTTATTTATAATGGCTCTGATTGTGCTGGGGCTTTTTACAATTATTGGGATATTCTACAGTGTACTGGTAGCGACAATGGTTGGACAAAGGATTTGGCAGCGGCATTATCATATACTTGCAAAAAGGATGCTAACAAAAGAATACGTTGTTGAGGATGTTGATGGTGAGATGACAGGATCTGATTGGTCCGCCCCACCTCTCCCACCTGAGCATGTTCAGCAGCTGAAAACCCTTGGCCTCCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

323

Amino Acids

35.92

Weight (kDa)

5.98

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 34 - 81 2.1e-10 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 731
Acc16I TGCGCA 2 cut(s) 536, 598
Acc36I ACCTGC 1 cut(s) 248
AccB7I CCANNNNNTGG 1 cut(s) 651
AccBSI CCGCTC 1 cut(s) 28
AccII CGCG 1 cut(s) 485
AciI CCGC 4 cut(s) 5, 26, 823, 912
AclWI GGATC 2 cut(s) 198, 907
AcoI YGGCCR 1 cut(s) 672
AcsI RAATTY 2 cut(s) 105, 302
AfaI GTAC 2 cut(s) 643, 786
AfiI CCNNNNNNNGG 1 cut(s) 651
AgsI TTSAA 1 cut(s) 301
AjnI CCWGG 2 cut(s) 586, 613
AluBI AGCT 5 cut(s) 203, 275, 355, 470, 946
AluI AGCT 5 cut(s) 203, 275, 355, 470, 946
Alw26I GTCTC 1 cut(s) 318
AlwI GGATC 2 cut(s) 198, 907
AlwNI CAGNNNCTG 2 cut(s) 902, 946
AoxI GGCC 3 cut(s) 79, 672, 958
ApeKI GCWGC 3 cut(s) 275, 820, 943
ApoI RAATTY 2 cut(s) 105, 302
ArsI GACNNNNNNTTYG 2 cut(s) 798, 830
AspLEI GCGC 2 cut(s) 537, 599
AspS9I GGNCC 3 cut(s) 80, 443, 909
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 3 cut(s) 40, 716, 899
AsuII TTCGAA 1 cut(s) 164
AvaII GGWCC 2 cut(s) 443, 909
BanII GRGCYC 1 cut(s) 57
BbvI GCAGC 3 cut(s) 262, 832, 955
BccI CCATC 3 cut(s) 116, 389, 878
BciT130I CCWGG 2 cut(s) 588, 615
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 1 cut(s) 318
BfmI CTRYAG 2 cut(s) 778, 965
BfuAI ACCTGC 1 cut(s) 248
BisI GCNGC 4 cut(s) 276, 821, 824, 944
BlpI GCTNAGC 1 cut(s) 549
BlsI GCNGC 4 cut(s) 277, 822, 825, 945
Bme1390I CCNGG 3 cut(s) 84, 588, 615
Bme18I GGWCC 2 cut(s) 443, 909
BmgT120I GGNCC 3 cut(s) 80, 443, 909
BmiI GGNNCC 3 cut(s) 82, 249, 444
BmrFI CCNGG 3 cut(s) 84, 588, 615
BmrI ACTGGG 1 cut(s) 51
BmsI GCATC 1 cut(s) 840
BmuI ACTGGG 1 cut(s) 51
Bpu10I CCTNAGC 1 cut(s) 930
Bpu1102I GCTNAGC 1 cut(s) 549
Bpu14I TTCGAA 1 cut(s) 164
BpuMI CCSGG 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 82, 955
Bsc4I CCNNNNNNNGG 1 cut(s) 651
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 3 cut(s) 57, 97, 792
Bse3DI GCAATG 1 cut(s) 229
BseBI CCWGG 2 cut(s) 588, 615
BseDI CCNNGG 2 cut(s) 82, 955
BseGI GGATG 3 cut(s) 13, 855, 883
BseLI CCNNNNNNNGG 1 cut(s) 651
BseMI GCAATG 1 cut(s) 229
BseMII CTCAG 2 cut(s) 563, 921
BseNI ACTGG 3 cut(s) 57, 97, 792
BseXI GCAGC 3 cut(s) 262, 832, 955
BseYI CCCAGC 2 cut(s) 651, 748
Bsh1236I CGCG 1 cut(s) 485
BshFI GGCC 3 cut(s) 81, 674, 960
BsiSI CCGG 3 cut(s) 78, 84, 675
BslFI GGGAC 2 cut(s) 172, 456
BslI CCNNNNNNNGG 1 cut(s) 651
BsmAI GTCTC 1 cut(s) 318
BsmFI GGGAC 2 cut(s) 172, 456
BsnI GGCC 3 cut(s) 81, 674, 960
Bsp119I TTCGAA 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 57
Bsp143I GATC 4 cut(s) 45, 63, 190, 899
Bsp1720I GCTNAGC 1 cut(s) 549
BspACI CCGC 4 cut(s) 5, 26, 823, 912
BspANI GGCC 3 cut(s) 81, 674, 960
BspCNI CTCAG 2 cut(s) 562, 922
BspFNI CGCG 1 cut(s) 485
BspLI GGNNCC 3 cut(s) 82, 249, 444
BspMI ACCTGC 1 cut(s) 248
BspPI GGATC 2 cut(s) 198, 907
BspT104I TTCGAA 1 cut(s) 164
BsrBI CCGCTC 1 cut(s) 28
BsrDI GCAATG 1 cut(s) 229
BsrFI RCCGGY 1 cut(s) 77
BsrI ACTGG 3 cut(s) 57, 97, 792
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 2 cut(s) 82, 955
BssMI GATC 4 cut(s) 45, 63, 190, 899
BssT1I CCWWGG 1 cut(s) 955
Bst2UI CCWGG 2 cut(s) 588, 615
Bst4CI ACNGT 2 cut(s) 641, 782
Bst6I CTCTTC 1 cut(s) 523
BstAPI GCANNNNNTGC 1 cut(s) 240
BstBI TTCGAA 1 cut(s) 164
BstC8I GCNNGC 2 cut(s) 79, 89
BstDEI CTNAG 2 cut(s) 549, 930
BstF5I GGATG 3 cut(s) 13, 855, 883
BstFNI CGCG 1 cut(s) 485
BstHHI GCGC 2 cut(s) 537, 599
BstKTI GATC 4 cut(s) 48, 66, 193, 902
BstMAI GTCTC 1 cut(s) 318
BstMBI GATC 4 cut(s) 45, 63, 190, 899
BstMWI GCNNNNNNNGC 7 cut(s) 108, 240, 371, 412, 671, 707, 940
BstNI CCWGG 2 cut(s) 588, 615
BstNSI RCATGY 2 cut(s) 651, 938
BstSCI CCNGG 3 cut(s) 82, 586, 613
BstSFI CTRYAG 2 cut(s) 778, 965
BstUI CGCG 1 cut(s) 485
BstV1I GCAGC 3 cut(s) 262, 832, 955
BstX2I RGATCY 1 cut(s) 899
BstYI RGATCY 1 cut(s) 899
BsuRI GGCC 3 cut(s) 81, 674, 960
BtgZI GCGATG 1 cut(s) 16
BtsCI GGATG 3 cut(s) 13, 855, 883
BtsIMutI CAGTG 3 cut(s) 64, 104, 787
BveI ACCTGC 1 cut(s) 248
Cac8I GCNNGC 2 cut(s) 79, 89
CaiI CAGNNNCTG 2 cut(s) 902, 946
CfoI GCGC 2 cut(s) 537, 599
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 3 cut(s) 80, 443, 909
CseI GACGC 1 cut(s) 26
Csp6I GTAC 2 cut(s) 642, 785
CviAII CATG 3 cut(s) 426, 648, 935
CviQI GTAC 2 cut(s) 642, 785
DdeI CTNAG 2 cut(s) 549, 930
DpnI GATC 4 cut(s) 47, 65, 192, 901
DpnII GATC 4 cut(s) 45, 63, 190, 899
EaeI YGGCCR 1 cut(s) 672
Eam1104I CTCTTC 1 cut(s) 523
EarI CTCTTC 1 cut(s) 523
EciI GGCGGA 2 cut(s) 20, 901
Eco130I CCWWGG 1 cut(s) 955
Eco24I GRGCYC 1 cut(s) 57
Eco47I GGWCC 2 cut(s) 443, 909
EcoRII CCWGG 2 cut(s) 586, 613
EcoT14I CCWWGG 1 cut(s) 955
EcoT38I GRGCYC 1 cut(s) 57
ErhI CCWWGG 1 cut(s) 955
FaeI CATG 3 cut(s) 429, 651, 938
FalI AAGNNNNNCTT 2 cut(s) 699, 731
FaqI GGGAC 2 cut(s) 172, 456
FatI CATG 3 cut(s) 425, 647, 934
Fnu4HI GCNGC 4 cut(s) 276, 821, 824, 944
FokI GGATG 3 cut(s) 20, 862, 890
FriOI GRGCYC 1 cut(s) 57
Fsp4HI GCNGC 4 cut(s) 276, 821, 824, 944
FspI TGCGCA 2 cut(s) 536, 598
GlaI GCGC 2 cut(s) 536, 598
GluI GCNGC 4 cut(s) 276, 821, 824, 944
GsaI CCCAGC 2 cut(s) 655, 752
HaeIII GGCC 3 cut(s) 81, 674, 960
HapII CCGG 3 cut(s) 78, 84, 675
HgaI GACGC 1 cut(s) 26
HhaI GCGC 2 cut(s) 537, 599
Hin1II CATG 3 cut(s) 429, 651, 938
Hin6I GCGC 2 cut(s) 535, 597
HinP1I GCGC 2 cut(s) 535, 597
HinfI GANTC 1 cut(s) 11
HpaII CCGG 3 cut(s) 78, 84, 675
HphI GGTGA 3 cut(s) 40, 716, 899
Hpy166II GTNNAC 3 cut(s) 175, 633, 785
Hpy188I TCNGA 4 cut(s) 16, 39, 741, 904
Hpy188III TCNNGA 2 cut(s) 316, 560
Hpy8I GTNNAC 3 cut(s) 175, 633, 785
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 1 cut(s) 206
HpyCH4III ACNGT 2 cut(s) 641, 782
HpyCH4IV ACGT 1 cut(s) 867
HpyCH4V TGCA 8 cut(s) 147, 153, 221, 234, 243, 425, 665, 842
HpyF10VI GCNNNNNNNGC 7 cut(s) 108, 240, 371, 412, 671, 707, 940
HpyF3I CTNAG 2 cut(s) 549, 930
HpySE526I ACGT 1 cut(s) 867
Hsp92II CATG 3 cut(s) 429, 651, 938
HspAI GCGC 2 cut(s) 535, 597
KroI GCCGGC 1 cut(s) 77
KroNI GCCGGC 1 cut(s) 79
Kzo9I GATC 4 cut(s) 45, 63, 190, 899
LmnI GCTCC 4 cut(s) 40, 145, 253, 467
Lsp1109I GCAGC 3 cut(s) 262, 832, 955
LweI GCATC 1 cut(s) 840
MaeII ACGT 1 cut(s) 867
MaeIII GTNAC 1 cut(s) 316
MalI GATC 4 cut(s) 47, 65, 192, 901
MbiI CCGCTC 1 cut(s) 28
MboI GATC 4 cut(s) 45, 63, 190, 899
MboII GAAGA 3 cut(s) 304, 472, 540
MflI RGATCY 1 cut(s) 899
MhlI GDGCHC 1 cut(s) 57
MluCI AATT 4 cut(s) 105, 302, 407, 762
MlyI GAGTC 1 cut(s) 5
MmeI TCCRAC 3 cut(s) 39, 62, 784
MnlI CCTC 5 cut(s) 80, 122, 689, 868, 930
MroNI GCCGGC 1 cut(s) 77
MspA1I CMGCKG 2 cut(s) 823, 946
MspI CCGG 3 cut(s) 78, 84, 675
MspR9I CCNGG 3 cut(s) 84, 588, 615
MvaI CCWGG 2 cut(s) 588, 615
MvnI CGCG 1 cut(s) 485
MwoI GCNNNNNNNGC 7 cut(s) 108, 240, 371, 412, 671, 707, 940
NaeI GCCGGC 1 cut(s) 79
NciI CCSGG 1 cut(s) 84
NdeII GATC 4 cut(s) 45, 63, 190, 899
NgoMIV GCCGGC 1 cut(s) 77
NlaIII CATG 3 cut(s) 429, 651, 938
NlaIV GGNNCC 3 cut(s) 82, 249, 444
NmuCI GTSAC 1 cut(s) 316
NsbI TGCGCA 2 cut(s) 536, 598
NspI RCATGY 2 cut(s) 651, 938
NspV TTCGAA 1 cut(s) 164
PdiI GCCGGC 1 cut(s) 79
PflMI CCANNNNNTGG 1 cut(s) 651
PkrI GCNGC 4 cut(s) 277, 822, 825, 945
PleI GAGTC 1 cut(s) 5
PpsI GAGTC 1 cut(s) 5
PsiI TTATAA 1 cut(s) 731
Psp6I CCWGG 2 cut(s) 586, 613
PspFI CCCAGC 2 cut(s) 651, 748
PspGI CCWGG 2 cut(s) 586, 613
PspN4I GGNNCC 3 cut(s) 82, 249, 444
PspPI GGNCC 3 cut(s) 80, 443, 909
PstNI CAGNNNCTG 2 cut(s) 902, 946
PsuI RGATCY 1 cut(s) 899
PvuII CAGCTG 1 cut(s) 946
RsaI GTAC 2 cut(s) 643, 786
RsaNI GTAC 2 cut(s) 642, 785
SatI GCNGC 4 cut(s) 276, 821, 824, 944
Sau3AI GATC 4 cut(s) 45, 63, 190, 899
Sau96I GGNCC 3 cut(s) 80, 443, 909
SchI GAGTC 1 cut(s) 5
ScrFI CCNGG 3 cut(s) 84, 588, 615
SduI GDGCHC 1 cut(s) 57
SfaNI GCATC 1 cut(s) 840
SfcI CTRYAG 2 cut(s) 778, 965
SfuI TTCGAA 1 cut(s) 164
SinI GGWCC 2 cut(s) 443, 909
Sse9I AATT 4 cut(s) 105, 302, 407, 762
SsiI CCGC 4 cut(s) 5, 26, 823, 912
StyD4I CCNGG 3 cut(s) 82, 586, 613
StyI CCWWGG 1 cut(s) 955
TaaI ACNGT 2 cut(s) 641, 782
TaiI ACGT 1 cut(s) 870
TaqI TCGA 5 cut(s) 66, 72, 114, 164, 559
TaqII GACCGA 1 cut(s) 540
TasI AATT 4 cut(s) 105, 302, 407, 762
TatI WGTACW 1 cut(s) 784
TauI GCSGC 1 cut(s) 826
TscAI CASTG 3 cut(s) 64, 104, 787
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 3 cut(s) 275, 820, 943
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 4 cut(s) 257, 325, 357, 499
TspRI CASTG 3 cut(s) 64, 104, 787
Van91I CCANNNNNTGG 1 cut(s) 651
VpaK11BI GGWCC 2 cut(s) 443, 909
XapI RAATTY 2 cut(s) 105, 302
XceI RCATGY 2 cut(s) 651, 938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.