MD15G1330300.v1.1

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009975
Physical Location & Seq
Reverse (-)
36394261 .. 36400407
6147 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1330300.v1.1.491

Sequence Viewer

Length: 969 bp
ATGGCCGATCACTCAGACGCTTCGCCTCTCATGCCTCCGAAGCAGATCACCGAGCCCAGCGAGATCGACCTCGAGGCGGGCCCTGGCGAACAAATCCAGTGCCGAATTTGCCTTGAAAGTGATGGTAGGGACTTCATTGCTCCCTGCAAGTGCAAAGGGACTTCCAAGTATGTCCACCGGGAGTGTTTGGATCATTGGAGAGCTGTAAAGGAAGGGTTTGCATTTTCTCATTGCACAACCTGCAAGGCTCCTTATCATTTGAGAGTTCATGTCATTGCTGATAGAAAATGGCGTACCTTGAAATTCAGATTCTTTGTGACGAGGGACATTATATTCATATTTCTGTCTGTTCAGCTTGTGATTGCTTCGTTGGCCTATTTGGTTTATTTAATAGATGCTTTACAGCAATTCTGGCTTCGTCTTGCATGGGGTTTTGACAGTGAGCTTAGCTTCTACTATATATGTGGAGCTCTATTGTTTTTCGCTTTGCTTGGGTTGTCTGGCTGCTTCATTACATGTTACGACCGTAGGGTGCGCAACGATTTGGCTCAGCCATGTCGTGAATTATGCCTTTGTTGCTGTCAACCTGGTGTGTGCGCAGACTGTCATTTACCTGGAACACTTTGTATGTGGACTGACTGTACTACATGCTGGGGAAGTTGTGCAAGTATGGCAGGTGAATGTGGTTGCTTGGGAGGCGCTGGTGAAGCAGGGCTACCGATATTATTTATAATGGCTCTGATTGTGCTGGGACTTTTCACCGTGATTGGGATATTCTATAGTGTCCTCGTAGCTACAATGGTTGGACAACGGATTTGGCAGCGGCATTATCATATACTTGCAAAAAGGATGCTGACAAAAGAATATGTTGTCGAGGATGTTGATGGTGAGATAACAGGATCTGATTGGTCTCCCCCACCTCTCCCAGCTGAGCATATTCAGCAGCTGAAAACGCTTGGCCTACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

323

Amino Acids

36.14

Weight (kDa)

6.2

Isoelectric Point (pI)

48.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 34 - 81 2e-10 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 731
AarI CACCTGC 1 cut(s) 665
AbsI CCTCGAGG 1 cut(s) 71
Acc16I TGCGCA 2 cut(s) 536, 598
Acc36I ACCTGC 2 cut(s) 248, 665
AciI CCGC 2 cut(s) 77, 823
AclWI GGATC 2 cut(s) 198, 907
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 105, 302
AfaI GTAC 2 cut(s) 295, 643
AfiI CCNNNNNNNGG 2 cut(s) 76, 768
AflIII ACRYGT 1 cut(s) 515
AgsI TTSAA 2 cut(s) 116, 301
AjnI CCWGG 3 cut(s) 82, 586, 613
AluBI AGCT 8 cut(s) 203, 355, 445, 450, 470, 794, 929, 946
AluI AGCT 8 cut(s) 203, 355, 445, 450, 470, 794, 929, 946
Alw21I GWGCWC 1 cut(s) 472
Alw26I GTCTC 1 cut(s) 915
AlwI GGATC 2 cut(s) 198, 907
AlwNI CAGNNNCTG 2 cut(s) 902, 946
Ama87I CYCGRG 1 cut(s) 71
AoxI GGCC 4 cut(s) 3, 79, 372, 958
ApaI GGGCCC 1 cut(s) 83
ApeKI GCWGC 3 cut(s) 504, 820, 943
ApoI RAATTY 2 cut(s) 105, 302
ArsI GACNNNNNNTTYG 2 cut(s) 798, 830
AspLEI GCGC 3 cut(s) 537, 599, 701
AspS9I GGNCC 2 cut(s) 79, 80
AsuC2I CCSGG 1 cut(s) 179
AsuHPI GGTGA 5 cut(s) 40, 689, 716, 751, 899
AvaI CYCGRG 1 cut(s) 71
BaeGI GKGCMC 1 cut(s) 83
BanII GRGCYC 3 cut(s) 57, 83, 472
BarI GAAGNNNNNNTAC 2 cut(s) 699, 731
Bbv12I GWGCWC 1 cut(s) 472
BbvI GCAGC 3 cut(s) 491, 832, 955
BccI CCATC 2 cut(s) 116, 878
BciT130I CCWGG 3 cut(s) 84, 588, 615
BcnI CCSGG 1 cut(s) 179
BcoDI GTCTC 1 cut(s) 915
BfmI CTRYAG 2 cut(s) 778, 965
BfoI RGCGCY 1 cut(s) 702
BfuAI ACCTGC 2 cut(s) 248, 665
BisI GCNGC 4 cut(s) 505, 821, 824, 944
BlpI GCTNAGC 3 cut(s) 446, 549, 930
BlsI GCNGC 4 cut(s) 506, 822, 825, 945
Bme1390I CCNGG 4 cut(s) 84, 179, 588, 615
BmeT110I CYCGRG 1 cut(s) 71
BmgT120I GGNCC 2 cut(s) 79, 80
BmiI GGNNCC 2 cut(s) 81, 249
BmrFI CCNGG 4 cut(s) 84, 179, 588, 615
BmsI GCATC 2 cut(s) 385, 840
Bpu1102I GCTNAGC 3 cut(s) 446, 549, 930
BpuMI CCSGG 1 cut(s) 179
BsaI GGTCTC 1 cut(s) 915
BsaJI CCNNGG 1 cut(s) 82
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 768
Bse1I ACTGG 1 cut(s) 97
Bse3DI GCAATG 3 cut(s) 135, 229, 273
BseBI CCWGG 3 cut(s) 84, 588, 615
BseDI CCNNGG 1 cut(s) 82
BseGI GGATG 2 cut(s) 855, 883
BseLI CCNNNNNNNGG 2 cut(s) 76, 768
BseMI GCAATG 3 cut(s) 135, 229, 273
BseMII CTCAG 3 cut(s) 27, 563, 921
BseNI ACTGG 1 cut(s) 97
BseSI GKGCMC 1 cut(s) 83
BseXI GCAGC 3 cut(s) 491, 832, 955
BseYI CCCAGC 4 cut(s) 56, 651, 748, 925
Bsh1285I CGRYCG 1 cut(s) 526
BshFI GGCC 4 cut(s) 5, 81, 374, 960
BsiEI CGRYCG 1 cut(s) 526
BsiHKAI GWGCWC 1 cut(s) 472
BsiHKCI CYCGRG 1 cut(s) 71
BsiSI CCGG 1 cut(s) 178
BslFI GGGAC 4 cut(s) 143, 172, 338, 765
BslI CCNNNNNNNGG 2 cut(s) 76, 768
BsmAI GTCTC 1 cut(s) 915
BsmFI GGGAC 4 cut(s) 143, 172, 338, 765
BsnI GGCC 4 cut(s) 5, 81, 374, 960
Bso31I GGTCTC 1 cut(s) 915
BsoBI CYCGRG 1 cut(s) 71
Bsp120I GGGCCC 1 cut(s) 79
Bsp1286I GDGCHC 3 cut(s) 57, 83, 472
Bsp143I GATC 5 cut(s) 7, 45, 63, 190, 899
Bsp1720I GCTNAGC 3 cut(s) 446, 549, 930
BspACI CCGC 2 cut(s) 77, 823
BspANI GGCC 4 cut(s) 5, 81, 374, 960
BspCNI CTCAG 3 cut(s) 26, 562, 922
BspLI GGNNCC 2 cut(s) 81, 249
BspMI ACCTGC 2 cut(s) 248, 665
BspPI GGATC 2 cut(s) 198, 907
BspTNI GGTCTC 1 cut(s) 915
BsrDI GCAATG 3 cut(s) 135, 229, 273
BsrI ACTGG 1 cut(s) 97
BssECI CCNNGG 1 cut(s) 82
BssMI GATC 5 cut(s) 7, 45, 63, 190, 899
Bst2UI CCWGG 3 cut(s) 84, 588, 615
Bst4CI ACNGT 5 cut(s) 440, 527, 605, 641, 763
BstAPI GCANNNNNTGC 1 cut(s) 240
BstC8I GCNNGC 1 cut(s) 79
BstDEI CTNAG 4 cut(s) 13, 446, 549, 930
BstF5I GGATG 2 cut(s) 855, 883
BstH2I RGCGCY 1 cut(s) 702
BstHHI GCGC 3 cut(s) 537, 599, 701
BstKTI GATC 5 cut(s) 10, 48, 66, 193, 902
BstMAI GTCTC 1 cut(s) 915
BstMBI GATC 5 cut(s) 7, 45, 63, 190, 899
BstMCI CGRYCG 1 cut(s) 526
BstNI CCWGG 3 cut(s) 84, 588, 615
BstNSI RCATGY 2 cut(s) 519, 651
BstSCI CCNGG 4 cut(s) 82, 177, 586, 613
BstSFI CTRYAG 2 cut(s) 778, 965
BstSLI GKGCMC 1 cut(s) 83
BstV1I GCAGC 3 cut(s) 491, 832, 955
BstX2I RGATCY 1 cut(s) 899
BstYI RGATCY 1 cut(s) 899
BsuRI GGCC 4 cut(s) 5, 81, 374, 960
BtsCI GGATG 2 cut(s) 855, 883
BtsIMutI CAGTG 2 cut(s) 104, 445
BveI ACCTGC 2 cut(s) 248, 665
Cac8I GCNNGC 1 cut(s) 79
CaiI CAGNNNCTG 2 cut(s) 902, 946
CfoI GCGC 3 cut(s) 537, 599, 701
Cfr13I GGNCC 2 cut(s) 79, 80
CseI GACGC 1 cut(s) 26
CsiI ACCWGGT 1 cut(s) 586
Csp6I GTAC 2 cut(s) 294, 642
CviAII CATG 6 cut(s) 31, 269, 426, 516, 555, 648
CviQI GTAC 2 cut(s) 294, 642
DdeI CTNAG 4 cut(s) 13, 446, 549, 930
DpnI GATC 5 cut(s) 9, 47, 65, 192, 901
DpnII GATC 5 cut(s) 7, 45, 63, 190, 899
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 470
Eco24I GRGCYC 3 cut(s) 57, 83, 472
Eco31I GGTCTC 1 cut(s) 915
Eco53kI GAGCTC 1 cut(s) 470
Eco88I CYCGRG 1 cut(s) 71
EcoICRI GAGCTC 1 cut(s) 470
EcoO109I RGGNCCY 1 cut(s) 80
EcoRII CCWGG 3 cut(s) 82, 586, 613
EcoT38I GRGCYC 3 cut(s) 57, 83, 472
FaeI CATG 6 cut(s) 34, 272, 429, 519, 558, 651
FaqI GGGAC 4 cut(s) 143, 172, 338, 765
FatI CATG 6 cut(s) 30, 268, 425, 515, 554, 647
FauI CCCGC 1 cut(s) 70
Fnu4HI GCNGC 4 cut(s) 505, 821, 824, 944
FokI GGATG 2 cut(s) 862, 890
FriOI GRGCYC 3 cut(s) 57, 83, 472
Fsp4HI GCNGC 4 cut(s) 505, 821, 824, 944
FspI TGCGCA 2 cut(s) 536, 598
GlaI GCGC 3 cut(s) 536, 598, 700
GluI GCNGC 4 cut(s) 505, 821, 824, 944
GsaI CCCAGC 4 cut(s) 60, 655, 752, 929
HaeII RGCGCY 1 cut(s) 702
HaeIII GGCC 4 cut(s) 5, 81, 374, 960
HapII CCGG 1 cut(s) 178
HgaI GACGC 1 cut(s) 26
HhaI GCGC 3 cut(s) 537, 599, 701
Hin1II CATG 6 cut(s) 34, 272, 429, 519, 558, 651
Hin6I GCGC 3 cut(s) 535, 597, 699
HinP1I GCGC 3 cut(s) 535, 597, 699
HincII GTYRAC 1 cut(s) 584
HindII GTYRAC 1 cut(s) 584
HinfI GANTC 1 cut(s) 309
HpaII CCGG 1 cut(s) 178
HphI GGTGA 5 cut(s) 40, 689, 716, 751, 899
Hpy166II GTNNAC 3 cut(s) 175, 584, 633
Hpy188I TCNGA 5 cut(s) 16, 39, 308, 741, 904
Hpy188III TCNNGA 1 cut(s) 560
Hpy8I GTNNAC 3 cut(s) 175, 584, 633
HpyAV CCTTC 1 cut(s) 206
HpyCH4III ACNGT 5 cut(s) 440, 527, 605, 641, 763
HpyCH4V TGCA 8 cut(s) 147, 153, 221, 234, 243, 425, 665, 842
HpyF3I CTNAG 4 cut(s) 13, 446, 549, 930
Hsp92II CATG 6 cut(s) 34, 272, 429, 519, 558, 651
HspAI GCGC 3 cut(s) 535, 597, 699
Kzo9I GATC 5 cut(s) 7, 45, 63, 190, 899
LmnI GCTCC 3 cut(s) 145, 253, 467
Lsp1109I GCAGC 3 cut(s) 491, 832, 955
LweI GCATC 2 cut(s) 385, 840
MabI ACCWGGT 1 cut(s) 586
MaeIII GTNAC 2 cut(s) 316, 518
MalI GATC 5 cut(s) 9, 47, 65, 192, 901
MboI GATC 5 cut(s) 7, 45, 63, 190, 899
MflI RGATCY 1 cut(s) 899
MhlI GDGCHC 3 cut(s) 57, 83, 472
MluCI AATT 4 cut(s) 105, 302, 407, 563
MmeI TCCRAC 1 cut(s) 784
MnlI CCTC 9 cut(s) 36, 45, 67, 80, 315, 689, 797, 868, 930
MseI TTAA 1 cut(s) 389
MspA1I CMGCKG 3 cut(s) 823, 929, 946
MspI CCGG 1 cut(s) 178
MspR9I CCNGG 4 cut(s) 84, 179, 588, 615
MvaI CCWGG 3 cut(s) 84, 588, 615
NciI CCSGG 1 cut(s) 179
NdeII GATC 5 cut(s) 7, 45, 63, 190, 899
NlaIII CATG 6 cut(s) 34, 272, 429, 519, 558, 651
NlaIV GGNNCC 2 cut(s) 81, 249
NmuCI GTSAC 1 cut(s) 316
NsbI TGCGCA 2 cut(s) 536, 598
NspI RCATGY 2 cut(s) 519, 651
PaeR7I CTCGAG 1 cut(s) 71
PaqCI CACCTGC 1 cut(s) 665
PciI ACATGT 1 cut(s) 515
PfeI GAWTC 1 cut(s) 309
PkrI GCNGC 4 cut(s) 506, 822, 825, 945
PscI ACATGT 1 cut(s) 515
PsiI TTATAA 1 cut(s) 731
Psp124BI GAGCTC 1 cut(s) 472
Psp6I CCWGG 3 cut(s) 82, 586, 613
PspFI CCCAGC 4 cut(s) 56, 651, 748, 925
PspGI CCWGG 3 cut(s) 82, 586, 613
PspN4I GGNNCC 2 cut(s) 81, 249
PspOMI GGGCCC 1 cut(s) 79
PspPI GGNCC 2 cut(s) 79, 80
PspXI VCTCGAGB 1 cut(s) 71
PstNI CAGNNNCTG 2 cut(s) 902, 946
PsuI RGATCY 1 cut(s) 899
PvuII CAGCTG 2 cut(s) 929, 946
RsaI GTAC 2 cut(s) 295, 643
RsaNI GTAC 2 cut(s) 294, 642
SacI GAGCTC 1 cut(s) 472
SaqAI TTAA 1 cut(s) 389
SatI GCNGC 4 cut(s) 505, 821, 824, 944
Sau3AI GATC 5 cut(s) 7, 45, 63, 190, 899
Sau96I GGNCC 2 cut(s) 79, 80
ScrFI CCNGG 4 cut(s) 84, 179, 588, 615
SduI GDGCHC 3 cut(s) 57, 83, 472
SexAI ACCWGGT 1 cut(s) 586
SfaNI GCATC 2 cut(s) 385, 840
SfcI CTRYAG 2 cut(s) 778, 965
Sfr274I CTCGAG 1 cut(s) 71
SlaI CTCGAG 1 cut(s) 71
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
Sse9I AATT 4 cut(s) 105, 302, 407, 563
SsiI CCGC 2 cut(s) 77, 823
SstI GAGCTC 1 cut(s) 472
StyD4I CCNGG 4 cut(s) 82, 177, 586, 613
TaaI ACNGT 5 cut(s) 440, 527, 605, 641, 763
TaqI TCGA 3 cut(s) 66, 72, 873
TasI AATT 4 cut(s) 105, 302, 407, 563
TatI WGTACW 1 cut(s) 641
TauI GCSGC 1 cut(s) 826
TfiI GAWTC 1 cut(s) 309
Tru1I TTAA 1 cut(s) 389
Tru9I TTAA 1 cut(s) 389
TscAI CASTG 2 cut(s) 104, 445
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 3 cut(s) 504, 820, 943
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 4 cut(s) 124, 257, 325, 499
TspGWI ACGGA 1 cut(s) 826
TspRI CASTG 2 cut(s) 104, 445
XapI RAATTY 2 cut(s) 105, 302
XceI RCATGY 2 cut(s) 519, 651
XhoI CTCGAG 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.