Prupe.6G177400_v2.0.a1

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0009975
Physical Location & Seq
Forward (+)
18250446 .. 18257573
7128 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G177400.1

Sequence Viewer

Length: 969 bp
ATGGTGGATCACTCAGACGCTTCGCCTCTCATCGCTCCGGCGCCGGTCGCCGAGCCCAGCGAGATCGACCTGGAAGCGGGCCCTGGTGAACAAATCCAATGCCGAATTTGCCTTGAAAGTGATGGTAGGGACTTCATTGCTCCTTGCAAATGCAAAGGGACTTCAAAGTATGTTCACCGGGAATGTTTGGATCATTGGAGAGCTGTAAAGGAAGGGTTTGCATTTGCTCATTGCACAACCTGCAAGGCTCCTTACCATTTGAGAGTTCATGTTATTGCTGATAGAAAATGGCGAACCTTGAAATTCCGATTCTTTGTCACTAGAGACATTATTTTTATATTTCTGGCCGTTCAGCTTGTGATTGCTTCATTGGCATATTTGGTGTATCTAATAGATGGTTTCCAGCAATTCTGGCTTCGTCTTGCATGGGGCTTTGACAGTGAGCTTAGTTTCTACTATATATCTGGAGCTCTGTTGTTTTTCGCTTTGCTTGGGTTGTCTGGGTGCTTCATTACTTGTTATGACCGAAGAGTGCGCAATGATTTGGCTCAGCCTTGTCGAGAATTATGCCTATGTTGCTGTCAGCCTGGTGTATGCGCAGACTGTCATTTACCAGGCACACTTTGTATGTGGACTGACTGTACCACGTGCTGGGGTAGCTGTGCAAGTATGGCAGGTGAATGTGGTTGCTTGGGAGGTGCTGGTGAAGCAGGGCTGCCAATACTATTTATAATGGCTTTGATTGTGCTGGGACTTTTTACAGTGATTGGCATATTCTACAGTGTGCTGGTAGCTACAATGGTTGGACAACGGATTTGGCAGCGGCATTACCATATACTTGCAAAAAGGATGCTGACAAAAGAATATGTTGTCGAGGATGTTGATGGTGAGATGACTGGATCTGATTGGTCTCCACCACCTCTCCCACCTGAGCATATTCAGCAGCTGAAAACGCTTGGCCTGCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

323

Amino Acids

36.01

Weight (kDa)

6.05

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 731
AarI CACCTGC 1 cut(s) 665
Acc16I TGCGCA 2 cut(s) 536, 598
Acc36I ACCTGC 2 cut(s) 248, 665
AccB1I GGYRCC 1 cut(s) 40
AccB7I CCANNNNNTGG 1 cut(s) 651
AciI CCGC 2 cut(s) 77, 823
AclWI GGATC 3 cut(s) 15, 198, 907
AcoI YGGCCR 1 cut(s) 345
AcsI RAATTY 2 cut(s) 105, 302
AcvI CACGTG 1 cut(s) 648
AcyI GRCGYC 1 cut(s) 41
AfaI GTAC 1 cut(s) 643
AfiI CCNNNNNNNGG 2 cut(s) 76, 651
AgsI TTSAA 3 cut(s) 116, 165, 301
AjnI CCWGG 4 cut(s) 69, 82, 586, 613
AluBI AGCT 7 cut(s) 203, 355, 445, 470, 660, 794, 946
AluI AGCT 7 cut(s) 203, 355, 445, 470, 660, 794, 946
Alw21I GWGCWC 1 cut(s) 472
Alw26I GTCTC 2 cut(s) 318, 915
AlwI GGATC 3 cut(s) 15, 198, 907
AlwNI CAGNNNCTG 1 cut(s) 946
AoxI GGCC 3 cut(s) 79, 345, 958
ApaI GGGCCC 1 cut(s) 83
ApeKI GCWGC 3 cut(s) 715, 820, 943
ApoI RAATTY 2 cut(s) 105, 302
ArsI GACNNNNNNTTYG 2 cut(s) 798, 830
AspLEI GCGC 3 cut(s) 43, 537, 599
AspS9I GGNCC 2 cut(s) 79, 80
AsuC2I CCSGG 1 cut(s) 179
AsuHPI GGTGA 5 cut(s) 98, 167, 689, 716, 899
BaeGI GKGCMC 1 cut(s) 83
BanI GGYRCC 1 cut(s) 40
BanII GRGCYC 3 cut(s) 57, 83, 472
BbrPI CACGTG 1 cut(s) 648
Bbv12I GWGCWC 1 cut(s) 472
BbvI GCAGC 3 cut(s) 702, 832, 955
BccI CCATC 3 cut(s) 116, 389, 878
BceAI ACGGC 1 cut(s) 332
BcgI CGANNNNNNTGC 2 cut(s) 549, 583
BciT130I CCWGG 4 cut(s) 71, 84, 588, 615
BcnI CCSGG 1 cut(s) 179
BcoDI GTCTC 2 cut(s) 318, 915
BfaI CTAG 1 cut(s) 321
BfmI CTRYAG 2 cut(s) 778, 965
BfoI RGCGCY 1 cut(s) 44
BfuAI ACCTGC 2 cut(s) 248, 665
BisI GCNGC 4 cut(s) 716, 821, 824, 944
BlpI GCTNAGC 1 cut(s) 549
BlsI GCNGC 4 cut(s) 717, 822, 825, 945
Bme1390I CCNGG 5 cut(s) 71, 84, 179, 588, 615
BmgT120I GGNCC 2 cut(s) 79, 80
BmiI GGNNCC 3 cut(s) 42, 81, 249
BmrFI CCNGG 5 cut(s) 71, 84, 179, 588, 615
BmsI GCATC 1 cut(s) 840
BpmI CTGGAG 1 cut(s) 486
Bpu10I CCTNAGC 1 cut(s) 930
Bpu1102I GCTNAGC 1 cut(s) 549
BpuMI CCSGG 1 cut(s) 179
BsaAI YACGTR 1 cut(s) 648
BsaHI GRCGYC 1 cut(s) 41
BsaI GGTCTC 1 cut(s) 915
BsaJI CCNNGG 1 cut(s) 82
BsaXI ACNNNNNCTCC 2 cut(s) 906, 936
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 651
Bse118I RCCGGY 1 cut(s) 43
Bse1I ACTGG 1 cut(s) 901
Bse3DI GCAATG 3 cut(s) 135, 229, 544
BseBI CCWGG 4 cut(s) 71, 84, 588, 615
BseDI CCNNGG 1 cut(s) 82
BseGI GGATG 2 cut(s) 855, 883
BseLI CCNNNNNNNGG 2 cut(s) 76, 651
BseMI GCAATG 3 cut(s) 135, 229, 544
BseMII CTCAG 3 cut(s) 27, 563, 921
BseNI ACTGG 1 cut(s) 901
BseSI GKGCMC 1 cut(s) 83
BseXI GCAGC 3 cut(s) 702, 832, 955
BseYI CCCAGC 3 cut(s) 56, 651, 748
Bsh1285I CGRYCG 1 cut(s) 48
BshFI GGCC 3 cut(s) 81, 347, 960
BshNI GGYRCC 1 cut(s) 40
BsiEI CGRYCG 1 cut(s) 48
BsiHKAI GWGCWC 1 cut(s) 472
BsiSI CCGG 3 cut(s) 38, 44, 178
BslFI GGGAC 3 cut(s) 143, 172, 765
BslI CCNNNNNNNGG 2 cut(s) 76, 651
BsmAI GTCTC 2 cut(s) 318, 915
BsmFI GGGAC 3 cut(s) 143, 172, 765
BsnI GGCC 3 cut(s) 81, 347, 960
Bso31I GGTCTC 1 cut(s) 915
Bsp120I GGGCCC 1 cut(s) 79
Bsp1286I GDGCHC 3 cut(s) 57, 83, 472
Bsp143I GATC 4 cut(s) 7, 63, 190, 899
Bsp1720I GCTNAGC 1 cut(s) 549
BspACI CCGC 2 cut(s) 77, 823
BspANI GGCC 3 cut(s) 81, 347, 960
BspCNI CTCAG 3 cut(s) 26, 562, 922
BspLI GGNNCC 3 cut(s) 42, 81, 249
BspMI ACCTGC 2 cut(s) 248, 665
BspPI GGATC 3 cut(s) 15, 198, 907
BspT107I GGYRCC 1 cut(s) 40
BspTNI GGTCTC 1 cut(s) 915
BsrDI GCAATG 3 cut(s) 135, 229, 544
BsrFI RCCGGY 1 cut(s) 43
BsrI ACTGG 1 cut(s) 901
BssAI RCCGGY 1 cut(s) 43
BssECI CCNNGG 1 cut(s) 82
BssMI GATC 4 cut(s) 7, 63, 190, 899
BssNI GRCGYC 1 cut(s) 41
Bst2UI CCWGG 4 cut(s) 71, 84, 588, 615
Bst4CI ACNGT 5 cut(s) 440, 605, 641, 763, 782
Bst6I CTCTTC 1 cut(s) 523
BstACI GRCGYC 1 cut(s) 41
BstAPI GCANNNNNTGC 1 cut(s) 240
BstBAI YACGTR 1 cut(s) 648
BstC8I GCNNGC 2 cut(s) 79, 962
BstDEI CTNAG 4 cut(s) 13, 446, 549, 930
BstF5I GGATG 2 cut(s) 855, 883
BstH2I RGCGCY 1 cut(s) 44
BstHHI GCGC 3 cut(s) 43, 537, 599
BstKTI GATC 4 cut(s) 10, 66, 193, 902
BstMAI GTCTC 2 cut(s) 318, 915
BstMBI GATC 4 cut(s) 7, 63, 190, 899
BstMCI CGRYCG 1 cut(s) 48
BstNI CCWGG 4 cut(s) 71, 84, 588, 615
BstSCI CCNGG 5 cut(s) 69, 82, 177, 586, 613
BstSFI CTRYAG 2 cut(s) 778, 965
BstSLI GKGCMC 1 cut(s) 83
BstV1I GCAGC 3 cut(s) 702, 832, 955
BstX2I RGATCY 1 cut(s) 899
BstYI RGATCY 1 cut(s) 899
BsuRI GGCC 3 cut(s) 81, 347, 960
BtgZI GCGATG 1 cut(s) 16
BtsCI GGATG 2 cut(s) 855, 883
BtsIMutI CAGTG 3 cut(s) 445, 768, 787
BveI ACCTGC 2 cut(s) 248, 665
Cac8I GCNNGC 2 cut(s) 79, 962
CaiI CAGNNNCTG 1 cut(s) 946
CfoI GCGC 3 cut(s) 43, 537, 599
Cfr10I RCCGGY 1 cut(s) 43
Cfr13I GGNCC 2 cut(s) 79, 80
CseI GACGC 1 cut(s) 26
Csp6I GTAC 1 cut(s) 642
CviAII CATG 2 cut(s) 269, 426
CviQI GTAC 1 cut(s) 642
DdeI CTNAG 4 cut(s) 13, 446, 549, 930
DinI GGCGCC 1 cut(s) 42
DpnI GATC 4 cut(s) 9, 65, 192, 901
DpnII GATC 4 cut(s) 7, 63, 190, 899
EaeI YGGCCR 1 cut(s) 345
Eam1104I CTCTTC 1 cut(s) 523
EarI CTCTTC 1 cut(s) 523
Ecl136II GAGCTC 1 cut(s) 470
Eco24I GRGCYC 3 cut(s) 57, 83, 472
Eco31I GGTCTC 1 cut(s) 915
Eco53kI GAGCTC 1 cut(s) 470
Eco72I CACGTG 1 cut(s) 648
EcoICRI GAGCTC 1 cut(s) 470
EcoO109I RGGNCCY 1 cut(s) 80
EcoRII CCWGG 4 cut(s) 69, 82, 586, 613
EcoT38I GRGCYC 3 cut(s) 57, 83, 472
EgeI GGCGCC 1 cut(s) 42
EheI GGCGCC 1 cut(s) 42
FaeI CATG 2 cut(s) 272, 429
FaqI GGGAC 3 cut(s) 143, 172, 765
FatI CATG 2 cut(s) 268, 425
FauI CCCGC 1 cut(s) 70
Fnu4HI GCNGC 4 cut(s) 716, 821, 824, 944
FokI GGATG 2 cut(s) 862, 890
FriOI GRGCYC 3 cut(s) 57, 83, 472
Fsp4HI GCNGC 4 cut(s) 716, 821, 824, 944
FspBI CTAG 1 cut(s) 321
FspI TGCGCA 2 cut(s) 536, 598
GlaI GCGC 3 cut(s) 42, 536, 598
GluI GCNGC 4 cut(s) 716, 821, 824, 944
GsaI CCCAGC 3 cut(s) 60, 655, 752
GsuI CTGGAG 1 cut(s) 486
HaeII RGCGCY 1 cut(s) 44
HaeIII GGCC 3 cut(s) 81, 347, 960
HapII CCGG 3 cut(s) 38, 44, 178
HgaI GACGC 1 cut(s) 26
HhaI GCGC 3 cut(s) 43, 537, 599
Hin1I GRCGYC 1 cut(s) 41
Hin1II CATG 2 cut(s) 272, 429
Hin6I GCGC 3 cut(s) 41, 535, 597
HinP1I GCGC 3 cut(s) 41, 535, 597
HinfI GANTC 1 cut(s) 309
HpaII CCGG 3 cut(s) 38, 44, 178
HphI GGTGA 5 cut(s) 98, 167, 689, 716, 899
Hpy166II GTNNAC 3 cut(s) 89, 175, 633
Hpy188I TCNGA 3 cut(s) 16, 308, 904
Hpy188III TCNNGA 2 cut(s) 465, 560
Hpy8I GTNNAC 3 cut(s) 89, 175, 633
HpyAV CCTTC 1 cut(s) 206
HpyCH4III ACNGT 5 cut(s) 440, 605, 641, 763, 782
HpyCH4IV ACGT 1 cut(s) 647
HpyCH4V TGCA 8 cut(s) 147, 153, 221, 234, 243, 425, 665, 842
HpyF3I CTNAG 4 cut(s) 13, 446, 549, 930
HpySE526I ACGT 1 cut(s) 647
Hsp92I GRCGYC 1 cut(s) 41
Hsp92II CATG 2 cut(s) 272, 429
HspAI GCGC 3 cut(s) 41, 535, 597
KasI GGCGCC 1 cut(s) 40
Kzo9I GATC 4 cut(s) 7, 63, 190, 899
LmnI GCTCC 4 cut(s) 40, 145, 253, 467
Lsp1109I GCAGC 3 cut(s) 702, 832, 955
LweI GCATC 1 cut(s) 840
MaeI CTAG 1 cut(s) 321
MaeII ACGT 1 cut(s) 647
MaeIII GTNAC 1 cut(s) 316
MalI GATC 4 cut(s) 9, 65, 192, 901
MboI GATC 4 cut(s) 7, 63, 190, 899
MboII GAAGA 1 cut(s) 540
MflI RGATCY 1 cut(s) 899
MhlI GDGCHC 3 cut(s) 57, 83, 472
MluCI AATT 4 cut(s) 105, 302, 407, 563
Mly113I GGCGCC 1 cut(s) 41
MmeI TCCRAC 1 cut(s) 784
MnlI CCTC 4 cut(s) 36, 689, 868, 930
MspA1I CMGCKG 2 cut(s) 823, 946
MspI CCGG 3 cut(s) 38, 44, 178
MspR9I CCNGG 5 cut(s) 71, 84, 179, 588, 615
MvaI CCWGG 4 cut(s) 71, 84, 588, 615
NarI GGCGCC 1 cut(s) 41
NciI CCSGG 1 cut(s) 179
NdeII GATC 4 cut(s) 7, 63, 190, 899
NlaIII CATG 2 cut(s) 272, 429
NlaIV GGNNCC 3 cut(s) 42, 81, 249
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 1 cut(s) 316
NsbI TGCGCA 2 cut(s) 536, 598
PaqCI CACCTGC 1 cut(s) 665
PfeI GAWTC 1 cut(s) 309
PflMI CCANNNNNTGG 1 cut(s) 651
PkrI GCNGC 4 cut(s) 717, 822, 825, 945
PluTI GGCGCC 1 cut(s) 44
PmaCI CACGTG 1 cut(s) 648
PmlI CACGTG 1 cut(s) 648
Ppu21I YACGTR 1 cut(s) 648
PsiI TTATAA 1 cut(s) 731
Psp124BI GAGCTC 1 cut(s) 472
Psp6I CCWGG 4 cut(s) 69, 82, 586, 613
PspCI CACGTG 1 cut(s) 648
PspFI CCCAGC 3 cut(s) 56, 651, 748
PspGI CCWGG 4 cut(s) 69, 82, 586, 613
PspN4I GGNNCC 3 cut(s) 42, 81, 249
PspOMI GGGCCC 1 cut(s) 79
PspPI GGNCC 2 cut(s) 79, 80
PstNI CAGNNNCTG 1 cut(s) 946
PsuI RGATCY 1 cut(s) 899
PvuII CAGCTG 1 cut(s) 946
RsaI GTAC 1 cut(s) 643
RsaNI GTAC 1 cut(s) 642
SacI GAGCTC 1 cut(s) 472
SatI GCNGC 4 cut(s) 716, 821, 824, 944
Sau3AI GATC 4 cut(s) 7, 63, 190, 899
Sau96I GGNCC 2 cut(s) 79, 80
ScrFI CCNGG 5 cut(s) 71, 84, 179, 588, 615
SduI GDGCHC 3 cut(s) 57, 83, 472
SfaNI GCATC 1 cut(s) 840
SfcI CTRYAG 2 cut(s) 778, 965
SfoI GGCGCC 1 cut(s) 42
Sse9I AATT 4 cut(s) 105, 302, 407, 563
SsiI CCGC 2 cut(s) 77, 823
SspDI GGCGCC 1 cut(s) 40
SspMI CTAG 1 cut(s) 321
SstI GAGCTC 1 cut(s) 472
StyD4I CCNGG 5 cut(s) 69, 82, 177, 586, 613
TaaI ACNGT 5 cut(s) 440, 605, 641, 763, 782
TaiI ACGT 1 cut(s) 650
TaqI TCGA 3 cut(s) 66, 559, 873
TaqII GACCGA 1 cut(s) 540
TasI AATT 4 cut(s) 105, 302, 407, 563
TauI GCSGC 1 cut(s) 826
TfiI GAWTC 1 cut(s) 309
TscAI CASTG 3 cut(s) 445, 768, 787
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 3 cut(s) 715, 820, 943
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 4 cut(s) 124, 257, 357, 499
TspGWI ACGGA 1 cut(s) 826
TspRI CASTG 3 cut(s) 445, 768, 787
Van91I CCANNNNNTGG 1 cut(s) 651
XapI RAATTY 2 cut(s) 105, 302
XspI CTAG 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.