Rw2G020100

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0009975
Physical Location & Seq
Reverse (-)
27223667 .. 27230575
6909 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G020100.1

Sequence Viewer

Length: 870 bp
ATGGCGGATGAGTCTGACGCTTCGCCTCTCATCGCTCCGATCCCGATCACCGACCCCAGTGAGATCGACCTCGAAGCCGGTCCCGGCGAGCAAATCCAGTGCCGAATTTGCCTTGAAAGTGATGGTAGGGATTTTATTGCTCCTTGCAAATGCAAAGGGACTTCAAAGTATGTTCATAGGGAGTGTTTGGATCATTGGAGAGCTGTAAAGGAAGGTTTTGCATTTGCTCATTGCACAACCTGCAAGGCTCCTTACCATTTGAGAGTTCATCAAGCTGCCGATAGGAAATGGCGAACCTTGAAATTTCGATTCTTTGTGACCAGAGACATTATATTTATATTTCTCGCTGTCCAGCTTGTGATTGCTTCATTGGCATATTTGGTGTTTTTAATAGATGGTTTCCAGCAATTTTGGCTTCGTCTTGCATGGGGTTTTGATAGTGAACCAAGTTTCTACTATATTTGTGGAGCTTTGCTGTTTTTCGCTTTGCTTGGGTTATCTGGATGCTTCATTACTTGTTATGACCGAAGAGTGCGCAACGATTTGGCTCAGCCTTGTCGAGAGTTATGTCTTTGTTGCTGTCAGCCTGGTGTCTGCGCAGATTGTCATTTACCTGGCACTCTTTGTATGTGGACTGACTGTACCACATGCTGGGGAAGTTGTGCAAGTATGGCTGGAGAATGTGGTTGCTTGGGAGGTGCTGGTGAAGCAGGGCTTCCAATATTATTTATAATGGCTCTGATTGTGCTGGGGCTTTTCACAATTATTGGGATATTCTACAGTGTACTGGTAGCTACAATGGTTGGACAAAGGATTTGGCAGCGGCATTATCATATACTTGCAAAAAGGATGCTGACAAAAGTGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

289

Amino Acids

32.34

Weight (kDa)

7.0

Isoelectric Point (pI)

43.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 34 - 81 1.8e-10 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 731
Acc16I TGCGCA 2 cut(s) 536, 598
Acc36I ACCTGC 1 cut(s) 248
AccB7I CCANNNNNTGG 1 cut(s) 651
AciI CCGC 2 cut(s) 5, 823
AclWI GGATC 2 cut(s) 34, 198
AcsI RAATTY 2 cut(s) 105, 302
AfaI GTAC 2 cut(s) 643, 786
AfiI CCNNNNNNNGG 1 cut(s) 651
AgsI TTSAA 3 cut(s) 116, 165, 301
AjnI CCWGG 2 cut(s) 586, 613
AluBI AGCT 5 cut(s) 203, 275, 355, 470, 794
AluI AGCT 5 cut(s) 203, 275, 355, 470, 794
Alw26I GTCTC 1 cut(s) 318
AlwI GGATC 2 cut(s) 34, 198
ApeKI GCWGC 2 cut(s) 275, 820
ApoI RAATTY 2 cut(s) 105, 302
ArsI GACNNNNNNTTYG 2 cut(s) 798, 830
AspLEI GCGC 2 cut(s) 537, 599
AspS9I GGNCC 1 cut(s) 80
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 2 cut(s) 40, 716
AvaII GGWCC 1 cut(s) 80
BbvI GCAGC 2 cut(s) 262, 832
BccI CCATC 2 cut(s) 116, 389
BciT130I CCWGG 2 cut(s) 588, 615
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 1 cut(s) 318
BfmI CTRYAG 1 cut(s) 778
BfuAI ACCTGC 1 cut(s) 248
BisI GCNGC 3 cut(s) 276, 821, 824
BlpI GCTNAGC 1 cut(s) 549
BlsI GCNGC 3 cut(s) 277, 822, 825
Bme1390I CCNGG 3 cut(s) 84, 588, 615
Bme18I GGWCC 1 cut(s) 80
BmgT120I GGNCC 1 cut(s) 80
BmiI GGNNCC 2 cut(s) 82, 249
BmrFI CCNGG 3 cut(s) 84, 588, 615
BmrI ACTGGG 1 cut(s) 51
BmsI GCATC 2 cut(s) 494, 840
BmuI ACTGGG 1 cut(s) 51
BpmI CTGGAG 1 cut(s) 696
Bpu1102I GCTNAGC 1 cut(s) 549
BpuMI CCSGG 1 cut(s) 84
BsaBI GATNNNNATC 1 cut(s) 44
Bsc4I CCNNNNNNNGG 1 cut(s) 651
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 3 cut(s) 57, 97, 792
Bse3DI GCAATG 1 cut(s) 229
Bse8I GATNNNNATC 1 cut(s) 44
BseBI CCWGG 2 cut(s) 588, 615
BseGI GGATG 3 cut(s) 13, 509, 855
BseJI GATNNNNATC 1 cut(s) 44
BseLI CCNNNNNNNGG 1 cut(s) 651
BseMI GCAATG 1 cut(s) 229
BseMII CTCAG 1 cut(s) 563
BseNI ACTGG 3 cut(s) 57, 97, 792
BseXI GCAGC 2 cut(s) 262, 832
BseYI CCCAGC 2 cut(s) 651, 748
BsiSI CCGG 2 cut(s) 78, 84
BslFI GGGAC 2 cut(s) 66, 172
BslI CCNNNNNNNGG 1 cut(s) 651
BsmAI GTCTC 1 cut(s) 318
BsmFI GGGAC 2 cut(s) 66, 172
Bsp143I GATC 4 cut(s) 39, 45, 63, 190
Bsp1720I GCTNAGC 1 cut(s) 549
BspACI CCGC 2 cut(s) 5, 823
BspCNI CTCAG 1 cut(s) 562
BspLI GGNNCC 2 cut(s) 82, 249
BspMI ACCTGC 1 cut(s) 248
BspPI GGATC 2 cut(s) 34, 198
BsrDI GCAATG 1 cut(s) 229
BsrFI RCCGGY 1 cut(s) 77
BsrI ACTGG 3 cut(s) 57, 97, 792
BssAI RCCGGY 1 cut(s) 77
BssMI GATC 4 cut(s) 39, 45, 63, 190
Bst2UI CCWGG 2 cut(s) 588, 615
Bst4CI ACNGT 2 cut(s) 641, 782
Bst6I CTCTTC 1 cut(s) 523
BstAPI GCANNNNNTGC 1 cut(s) 240
BstC8I GCNNGC 1 cut(s) 89
BstDEI CTNAG 1 cut(s) 549
BstF5I GGATG 3 cut(s) 13, 509, 855
BstHHI GCGC 2 cut(s) 537, 599
BstKTI GATC 4 cut(s) 42, 48, 66, 193
BstMAI GTCTC 1 cut(s) 318
BstMBI GATC 4 cut(s) 39, 45, 63, 190
BstMWI GCNNNNNNNGC 6 cut(s) 108, 240, 371, 412, 671, 707
BstNI CCWGG 2 cut(s) 588, 615
BstNSI RCATGY 1 cut(s) 651
BstSCI CCNGG 3 cut(s) 82, 586, 613
BstSFI CTRYAG 1 cut(s) 778
BstV1I GCAGC 2 cut(s) 262, 832
BtgZI GCGATG 1 cut(s) 16
BtsCI GGATG 3 cut(s) 13, 509, 855
BtsIMutI CAGTG 3 cut(s) 64, 104, 787
BveI ACCTGC 1 cut(s) 248
Cac8I GCNNGC 1 cut(s) 89
CfoI GCGC 2 cut(s) 537, 599
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 1 cut(s) 80
CseI GACGC 1 cut(s) 26
Csp6I GTAC 2 cut(s) 642, 785
CviAII CATG 2 cut(s) 426, 648
CviQI GTAC 2 cut(s) 642, 785
DdeI CTNAG 1 cut(s) 549
DpnI GATC 4 cut(s) 41, 47, 65, 192
DpnII GATC 4 cut(s) 39, 45, 63, 190
Eam1104I CTCTTC 1 cut(s) 523
EarI CTCTTC 1 cut(s) 523
EciI GGCGGA 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 80
EcoRII CCWGG 2 cut(s) 586, 613
FaeI CATG 2 cut(s) 429, 651
FalI AAGNNNNNCTT 2 cut(s) 699, 731
FaqI GGGAC 2 cut(s) 66, 172
FatI CATG 2 cut(s) 425, 647
Fnu4HI GCNGC 3 cut(s) 276, 821, 824
FokI GGATG 3 cut(s) 20, 516, 862
Fsp4HI GCNGC 3 cut(s) 276, 821, 824
FspI TGCGCA 2 cut(s) 536, 598
GlaI GCGC 2 cut(s) 536, 598
GluI GCNGC 3 cut(s) 276, 821, 824
GsaI CCCAGC 2 cut(s) 655, 752
GsuI CTGGAG 1 cut(s) 696
HapII CCGG 2 cut(s) 78, 84
HgaI GACGC 1 cut(s) 26
HhaI GCGC 2 cut(s) 537, 599
Hin1II CATG 2 cut(s) 429, 651
Hin6I GCGC 2 cut(s) 535, 597
HinP1I GCGC 2 cut(s) 535, 597
HinfI GANTC 2 cut(s) 11, 309
HpaII CCGG 2 cut(s) 78, 84
HphI GGTGA 2 cut(s) 40, 716
Hpy166II GTNNAC 3 cut(s) 443, 633, 785
Hpy188I TCNGA 3 cut(s) 16, 39, 741
Hpy188III TCNNGA 3 cut(s) 43, 501, 560
Hpy8I GTNNAC 3 cut(s) 443, 633, 785
HpyAV CCTTC 1 cut(s) 206
HpyCH4III ACNGT 2 cut(s) 641, 782
HpyCH4V TGCA 8 cut(s) 147, 153, 221, 234, 243, 425, 665, 842
HpyF10VI GCNNNNNNNGC 6 cut(s) 108, 240, 371, 412, 671, 707
HpyF3I CTNAG 1 cut(s) 549
Hsp92II CATG 2 cut(s) 429, 651
HspAI GCGC 2 cut(s) 535, 597
Kzo9I GATC 4 cut(s) 39, 45, 63, 190
LmnI GCTCC 4 cut(s) 40, 145, 253, 467
Lsp1109I GCAGC 2 cut(s) 262, 832
LweI GCATC 2 cut(s) 494, 840
MaeIII GTNAC 1 cut(s) 316
MalI GATC 4 cut(s) 41, 47, 65, 192
MboI GATC 4 cut(s) 39, 45, 63, 190
MboII GAAGA 1 cut(s) 540
MluCI AATT 4 cut(s) 105, 302, 407, 762
MlyI GAGTC 1 cut(s) 20
MmeI TCCRAC 1 cut(s) 784
MnlI CCTC 3 cut(s) 36, 80, 689
MseI TTAA 1 cut(s) 389
MspA1I CMGCKG 1 cut(s) 823
MspI CCGG 2 cut(s) 78, 84
MspR9I CCNGG 3 cut(s) 84, 588, 615
MvaI CCWGG 2 cut(s) 588, 615
MwoI GCNNNNNNNGC 6 cut(s) 108, 240, 371, 412, 671, 707
NciI CCSGG 1 cut(s) 84
NdeII GATC 4 cut(s) 39, 45, 63, 190
NlaIII CATG 2 cut(s) 429, 651
NlaIV GGNNCC 2 cut(s) 82, 249
NmuCI GTSAC 1 cut(s) 316
NsbI TGCGCA 2 cut(s) 536, 598
NspI RCATGY 1 cut(s) 651
PfeI GAWTC 1 cut(s) 309
PflMI CCANNNNNTGG 1 cut(s) 651
PkrI GCNGC 3 cut(s) 277, 822, 825
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
PsiI TTATAA 1 cut(s) 731
Psp6I CCWGG 2 cut(s) 586, 613
PspFI CCCAGC 2 cut(s) 651, 748
PspGI CCWGG 2 cut(s) 586, 613
PspN4I GGNNCC 2 cut(s) 82, 249
PspPI GGNCC 1 cut(s) 80
RsaI GTAC 2 cut(s) 643, 786
RsaNI GTAC 2 cut(s) 642, 785
SaqAI TTAA 1 cut(s) 389
SatI GCNGC 3 cut(s) 276, 821, 824
Sau3AI GATC 4 cut(s) 39, 45, 63, 190
Sau96I GGNCC 1 cut(s) 80
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 3 cut(s) 84, 588, 615
SfaNI GCATC 2 cut(s) 494, 840
SfcI CTRYAG 1 cut(s) 778
SinI GGWCC 1 cut(s) 80
Sse9I AATT 4 cut(s) 105, 302, 407, 762
SsiI CCGC 2 cut(s) 5, 823
SspI AATATT 1 cut(s) 723
StyD4I CCNGG 3 cut(s) 82, 586, 613
TaaI ACNGT 2 cut(s) 641, 782
TaqI TCGA 4 cut(s) 66, 72, 307, 559
TaqII GACCGA 1 cut(s) 540
TasI AATT 4 cut(s) 105, 302, 407, 762
TatI WGTACW 1 cut(s) 784
TauI GCSGC 1 cut(s) 826
TfiI GAWTC 1 cut(s) 309
Tru1I TTAA 1 cut(s) 389
Tru9I TTAA 1 cut(s) 389
TscAI CASTG 3 cut(s) 64, 104, 787
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 2 cut(s) 275, 820
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 4 cut(s) 164, 257, 357, 499
TspRI CASTG 3 cut(s) 64, 104, 787
Van91I CCANNNNNTGG 1 cut(s) 651
VpaK11BI GGWCC 1 cut(s) 80
XapI RAATTY 2 cut(s) 105, 302
XceI RCATGY 1 cut(s) 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.