Rh2CG262400

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0009975
Physical Location & Seq
Reverse (-)
28172992 .. 28179751
6760 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG262400.1

Sequence Viewer

Length: 696 bp
ATGGCGGATGAGTCTGACGCTTCGCCTCTCATCGCTCCGATCCCGATCACCGACCCCAGTGAGATCGACCTCGAAGCCGGTCCCGGCGAGCAAATCCAGTGCCGAATTTGCCTTGAAAGTGATGGTAGGGATTTTATTGCTCCTTGCAAATGCAAAGGGACTTCAAAGTATGTTCACAGGGAGTGTTTGGATCATTGGAGAGCTGTAAAGGAAGGTTTTGCATTTGCTCATTGCACAACCTGCAAGGCTCCTTACCATTTGAGAGTTCATCAAGCTGCCGATAGGAAATGGCGAACCTTGAAATTTCGATTCTTTGTGACCAGAGACATTATATTTATATTTCTCGCTGTCCAGCTTGTGATTGCTTCATTGGCATATTTGGTGTTTTTAATAGATGGTTTCCAGCAATTTTGGCTTCGTCTTGCATGGGGTTTTGATAGTGAACCAAGTTTCTACTATATTTGTGGAGCTTTGCTGTTTTTCGCTTTGCTTGGGTTATCGGGATGCTTCATTACTTGTTATGACCGAAGAGTGCGCAACGATTTGGCTCAGCCTTGTCGAGAGTTATGTCTTTGTTGCTGTCAGCCTGGGTATAAGTTAAATTTGGGAATATATGACCTAGTTGGTACAAATGATTTGGTTTACTGGCTGTTTAGCAAATCATCATTATCATCATATTTTCTACCTCTAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

231

Amino Acids

26.41

Weight (kDa)

6.91

Isoelectric Point (pI)

34.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 34 - 81 1.3e-10 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 536
Acc36I ACCTGC 1 cut(s) 248
AciI CCGC 1 cut(s) 5
AclWI GGATC 2 cut(s) 34, 198
AcsI RAATTY 3 cut(s) 105, 302, 601
AfaI GTAC 1 cut(s) 628
AgsI TTSAA 3 cut(s) 116, 165, 301
AjnI CCWGG 1 cut(s) 586
AluBI AGCT 4 cut(s) 203, 275, 355, 470
AluI AGCT 4 cut(s) 203, 275, 355, 470
Alw26I GTCTC 1 cut(s) 318
AlwI GGATC 2 cut(s) 34, 198
ApeKI GCWGC 1 cut(s) 275
ApoI RAATTY 3 cut(s) 105, 302, 601
AspLEI GCGC 1 cut(s) 537
AspS9I GGNCC 1 cut(s) 80
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 1 cut(s) 40
AvaII GGWCC 1 cut(s) 80
BbvI GCAGC 1 cut(s) 262
BccI CCATC 2 cut(s) 116, 389
BciT130I CCWGG 1 cut(s) 588
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 1 cut(s) 318
BfaI CTAG 1 cut(s) 620
BfuAI ACCTGC 1 cut(s) 248
BisI GCNGC 1 cut(s) 276
BlpI GCTNAGC 1 cut(s) 549
BlsI GCNGC 1 cut(s) 277
Bme1390I CCNGG 2 cut(s) 84, 588
Bme18I GGWCC 1 cut(s) 80
BmgT120I GGNCC 1 cut(s) 80
BmiI GGNNCC 2 cut(s) 82, 249
BmrFI CCNGG 2 cut(s) 84, 588
BmrI ACTGGG 1 cut(s) 51
BmsI GCATC 1 cut(s) 494
BmuI ACTGGG 1 cut(s) 51
Bpu1102I GCTNAGC 1 cut(s) 549
BpuMI CCSGG 1 cut(s) 84
BsaBI GATNNNNATC 1 cut(s) 44
BsaJI CCNNGG 1 cut(s) 587
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 3 cut(s) 57, 97, 650
Bse3DI GCAATG 1 cut(s) 229
Bse8I GATNNNNATC 1 cut(s) 44
BseBI CCWGG 1 cut(s) 588
BseDI CCNNGG 1 cut(s) 587
BseGI GGATG 2 cut(s) 13, 509
BseJI GATNNNNATC 1 cut(s) 44
BseMI GCAATG 1 cut(s) 229
BseMII CTCAG 1 cut(s) 563
BseNI ACTGG 3 cut(s) 57, 97, 650
BseXI GCAGC 1 cut(s) 262
BsiSI CCGG 2 cut(s) 78, 84
BslFI GGGAC 2 cut(s) 66, 172
BsmAI GTCTC 1 cut(s) 318
BsmFI GGGAC 2 cut(s) 66, 172
Bsp143I GATC 4 cut(s) 39, 45, 63, 190
Bsp1720I GCTNAGC 1 cut(s) 549
BspACI CCGC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 562
BspLI GGNNCC 2 cut(s) 82, 249
BspMI ACCTGC 1 cut(s) 248
BspPI GGATC 2 cut(s) 34, 198
BsrDI GCAATG 1 cut(s) 229
BsrFI RCCGGY 1 cut(s) 77
BsrI ACTGG 3 cut(s) 57, 97, 650
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 1 cut(s) 587
BssMI GATC 4 cut(s) 39, 45, 63, 190
Bst2UI CCWGG 1 cut(s) 588
Bst6I CTCTTC 1 cut(s) 523
BstAPI GCANNNNNTGC 1 cut(s) 240
BstC8I GCNNGC 1 cut(s) 89
BstDEI CTNAG 1 cut(s) 549
BstF5I GGATG 2 cut(s) 13, 509
BstHHI GCGC 1 cut(s) 537
BstKTI GATC 4 cut(s) 42, 48, 66, 193
BstMAI GTCTC 1 cut(s) 318
BstMBI GATC 4 cut(s) 39, 45, 63, 190
BstMWI GCNNNNNNNGC 4 cut(s) 108, 240, 371, 412
BstNI CCWGG 1 cut(s) 588
BstSCI CCNGG 2 cut(s) 82, 586
BstV1I GCAGC 1 cut(s) 262
BtgZI GCGATG 1 cut(s) 16
BtsCI GGATG 2 cut(s) 13, 509
BtsIMutI CAGTG 2 cut(s) 64, 104
BveI ACCTGC 1 cut(s) 248
Cac8I GCNNGC 1 cut(s) 89
CfoI GCGC 1 cut(s) 537
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 1 cut(s) 80
CseI GACGC 1 cut(s) 26
Csp6I GTAC 1 cut(s) 627
CviAII CATG 1 cut(s) 426
CviQI GTAC 1 cut(s) 627
DdeI CTNAG 1 cut(s) 549
DpnI GATC 4 cut(s) 41, 47, 65, 192
DpnII GATC 4 cut(s) 39, 45, 63, 190
Eam1104I CTCTTC 1 cut(s) 523
EarI CTCTTC 1 cut(s) 523
EciI GGCGGA 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 80
EcoRII CCWGG 1 cut(s) 586
FaeI CATG 1 cut(s) 429
FaqI GGGAC 2 cut(s) 66, 172
FatI CATG 1 cut(s) 425
Fnu4HI GCNGC 1 cut(s) 276
FokI GGATG 2 cut(s) 20, 516
Fsp4HI GCNGC 1 cut(s) 276
FspBI CTAG 1 cut(s) 620
FspI TGCGCA 1 cut(s) 536
GlaI GCGC 1 cut(s) 536
GluI GCNGC 1 cut(s) 276
HapII CCGG 2 cut(s) 78, 84
HgaI GACGC 1 cut(s) 26
HhaI GCGC 1 cut(s) 537
Hin1II CATG 1 cut(s) 429
Hin6I GCGC 1 cut(s) 535
HinP1I GCGC 1 cut(s) 535
HinfI GANTC 2 cut(s) 11, 309
HpaII CCGG 2 cut(s) 78, 84
HphI GGTGA 1 cut(s) 40
Hpy166II GTNNAC 3 cut(s) 175, 443, 643
Hpy188I TCNGA 2 cut(s) 16, 39
Hpy188III TCNNGA 3 cut(s) 43, 501, 560
Hpy8I GTNNAC 3 cut(s) 175, 443, 643
HpyAV CCTTC 1 cut(s) 206
HpyCH4V TGCA 6 cut(s) 147, 153, 221, 234, 243, 425
HpyF10VI GCNNNNNNNGC 4 cut(s) 108, 240, 371, 412
HpyF3I CTNAG 1 cut(s) 549
Hsp92II CATG 1 cut(s) 429
HspAI GCGC 1 cut(s) 535
Kzo9I GATC 4 cut(s) 39, 45, 63, 190
LmnI GCTCC 4 cut(s) 40, 145, 253, 467
Lsp1109I GCAGC 1 cut(s) 262
LweI GCATC 1 cut(s) 494
MaeI CTAG 1 cut(s) 620
MaeIII GTNAC 1 cut(s) 316
MalI GATC 4 cut(s) 41, 47, 65, 192
MboI GATC 4 cut(s) 39, 45, 63, 190
MboII GAAGA 1 cut(s) 540
MluCI AATT 4 cut(s) 105, 302, 407, 601
MlyI GAGTC 1 cut(s) 20
MnlI CCTC 3 cut(s) 36, 80, 696
MseI TTAA 2 cut(s) 389, 599
MspI CCGG 2 cut(s) 78, 84
MspR9I CCNGG 2 cut(s) 84, 588
MvaI CCWGG 1 cut(s) 588
MwoI GCNNNNNNNGC 4 cut(s) 108, 240, 371, 412
NciI CCSGG 1 cut(s) 84
NdeII GATC 4 cut(s) 39, 45, 63, 190
NlaIII CATG 1 cut(s) 429
NlaIV GGNNCC 2 cut(s) 82, 249
NmuCI GTSAC 1 cut(s) 316
NsbI TGCGCA 1 cut(s) 536
PfeI GAWTC 1 cut(s) 309
PkrI GCNGC 1 cut(s) 277
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
Psp6I CCWGG 1 cut(s) 586
PspGI CCWGG 1 cut(s) 586
PspN4I GGNNCC 2 cut(s) 82, 249
PspPI GGNCC 1 cut(s) 80
RsaI GTAC 1 cut(s) 628
RsaNI GTAC 1 cut(s) 627
SaqAI TTAA 2 cut(s) 389, 599
SatI GCNGC 1 cut(s) 276
Sau3AI GATC 4 cut(s) 39, 45, 63, 190
Sau96I GGNCC 1 cut(s) 80
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 2 cut(s) 84, 588
SfaNI GCATC 1 cut(s) 494
SinI GGWCC 1 cut(s) 80
Sse9I AATT 4 cut(s) 105, 302, 407, 601
SsiI CCGC 1 cut(s) 5
SspMI CTAG 1 cut(s) 620
StyD4I CCNGG 2 cut(s) 82, 586
TaqI TCGA 4 cut(s) 66, 72, 307, 559
TaqII GACCGA 1 cut(s) 540
TasI AATT 4 cut(s) 105, 302, 407, 601
TfiI GAWTC 1 cut(s) 309
Tru1I TTAA 2 cut(s) 389, 599
Tru9I TTAA 2 cut(s) 389, 599
TscAI CASTG 2 cut(s) 64, 104
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 1 cut(s) 275
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 3 cut(s) 257, 357, 499
TspRI CASTG 2 cut(s) 64, 104
VpaK11BI GGWCC 1 cut(s) 80
XapI RAATTY 3 cut(s) 105, 302, 601
XspI CTAG 1 cut(s) 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.