AT2G23540

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0015376
Physical Location & Seq
Forward (+)
10024150 .. 10026258
2109 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G23540.1

Sequence Viewer

Length: 1164 bp
ATGGCCACAAGAGCTTCTACATCTAGCAGAGTCTCTCCAGCTTTCACTTTCCTCGTCATCTTCTTCCTCTTATCTCTCACTGCTTCAGTAGAAGCTGCTGGTCGTGGAGTTAACAATGACAAGAAAGGCGGCGGATTAGGAGCTTCTTTCATATTCGGAGATTCTCTAGTCGATGCCGGAAATAATAATTATCTATCGACGTTGTCTAGGGCTAATATGAAGCCTAATGGTATTGATTTCAAAGCCTCCGGAGGAACTCCTACCGGCCGGTTCACCAATGGACGGACCATCGGTGATATCGTTGGGGAAGAACTCGGATCAGCGAACTACGCGATCCCGTTCTTGGCACCAGACGCGAAGGGAAAAGCTTTATTAGCCGGAGTGAACTATGCATCTGGAGGAGGAGGAATCATGAATGCCACCGGGAGAATCTTTGTGAATAGATTAGGTATGGATGTACAAGTTGATTTCTTCAACACTACACGGAAACAGTTTGATGATCTACTTGGAAAAGAGAAAGCAAAAGATTACATAGCCAAGAAATCGATATTCTCAATCACTATAGGAGCAAATGATTTCCTCAACAATTATCTATTCCCACTACTCTCGGTAGGAACACGGTTCACTCAAACTCCCGATGATTTTATTGGCGACATGCTTGAACATCTACGCGACCAACTAACCAGGCTGTATCAATTGGATGCGAGGAAGTTTGTGATCGGGAACGTTGGACCGATTGGATGCATACCGTACCAGAAAACAATCAATCAATTAGACGAAAACGAGTGTGTGGATTTGGCTAATAAACTAGCAAATCAATACAATGTTCGGTTAAAGAGTTTATTAGAGGAACTAAACAAGAAACTTCCCGGAGCAATGTTTGTCCATGCAAACGTCTACGATCTCGTCATGGAGCTCATTACTAACTACGATAAATACGGGTTCAAAAGTGCGACAAAGGCTTGTTGCGGTAATGGAGGACAATACGCGGGTATAATTCCGTGCGGACCGACTTCGAGTTTGTGTGAAGAGAGGGATAAATATGTGTTTTGGGATCCTTATCATCCGAGTGAAGCAGCAAATGTCATCATCGCGAAGCAATTGTTATATGGTGATGTTAAAGTTATATCTCCAGTTAATCTCAGCAAACTTAGAGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

387

Amino Acids

42.08

Weight (kDa)

8.37

Isoelectric Point (pI)

18.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 50 - 368 1.7e-34 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23540
fragaria_vesca FvH4_1g14780
malus_domestica MD02G1161000.v1.1 MD15G1275100.v1.1
prunus_persica Prupe.7G142600_v2.0.a1
pyrus_communis pycom02g12710 pycom15g23820
rosa_chinensis RchiOBHm_Chr2g0103741
rosa_laevigata RLG00000017253
rosa_multiflora Rmu_sc0001852.1_g000002
rosa_roxburghii Rroxscaffold_2G00139440
rosa_rugosa Rorug02G0117000
rosa_samantha Rh2AG165800 Rh2BG173100 Rh2CG171800
rosa_wichuraiana Rw2G012990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 346
AccI GTMKAC 1 cut(s) 897
AccII CGCG 5 cut(s) 332, 356, 672, 989, 1094
AccIII TCCGGA 1 cut(s) 248
AciI CCGC 5 cut(s) 129, 132, 969, 989, 1005
AclI AACGTT 1 cut(s) 726
AclWI GGATC 4 cut(s) 325, 328, 1049, 1062
AcoI YGGCCR 2 cut(s) 3, 265
AcuI CTGAAG 1 cut(s) 69
AfaI GTAC 2 cut(s) 459, 752
AfiI CCNNNNNNNGG 2 cut(s) 282, 343
AgsI TTSAA 4 cut(s) 241, 475, 662, 946
AjnI CCWGG 1 cut(s) 683
AluBI AGCT 6 cut(s) 14, 41, 95, 143, 368, 916
AluI AGCT 6 cut(s) 14, 41, 95, 143, 368, 916
Alw21I GWGCWC 1 cut(s) 918
Alw26I GTCTC 1 cut(s) 37
AlwI GGATC 4 cut(s) 325, 328, 1049, 1062
Aor13HI TCCGGA 1 cut(s) 248
AoxI GGCC 2 cut(s) 3, 265
ApeKI GCWGC 2 cut(s) 95, 1076
ArsI GACNNNNNNTTYG 2 cut(s) 1003, 1035
AspS9I GGNCC 3 cut(s) 285, 731, 1007
AsuC2I CCSGG 2 cut(s) 424, 870
AsuHPI GGTGA 3 cut(s) 265, 305, 1124
AvaII GGWCC 3 cut(s) 285, 731, 1007
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 1054
BanI GGYRCC 1 cut(s) 346
BanII GRGCYC 1 cut(s) 918
Bbv12I GWGCWC 1 cut(s) 918
BbvI GCAGC 2 cut(s) 82, 1088
BccI CCATC 1 cut(s) 296
BciT130I CCWGG 1 cut(s) 685
BcnI CCSGG 2 cut(s) 424, 870
BcoDI GTCTC 1 cut(s) 37
BfaI CTAG 4 cut(s) 24, 167, 207, 809
BfmI CTRYAG 1 cut(s) 561
BisI GCNGC 3 cut(s) 96, 130, 1077
BlsI GCNGC 3 cut(s) 97, 131, 1078
Bme1390I CCNGG 3 cut(s) 424, 685, 870
Bme18I GGWCC 3 cut(s) 285, 731, 1007
BmgT120I GGNCC 3 cut(s) 285, 731, 1007
BmiI GGNNCC 2 cut(s) 348, 1056
BmrFI CCNGG 3 cut(s) 424, 685, 870
BmsI GCATC 4 cut(s) 163, 401, 691, 731
BpmI CTGGAG 3 cut(s) 21, 417, 1116
BpuMI CCSGG 2 cut(s) 424, 870
Bsa29I ATCGAT 1 cut(s) 545
BsaBI GATNNNNATC 1 cut(s) 1059
BsaWI WCCGGW 1 cut(s) 248
BsaXI ACNNNNNCTCC 2 cut(s) 616, 646
Bsc4I CCNNNNNNNGG 2 cut(s) 282, 343
Bse118I RCCGGY 2 cut(s) 263, 267
Bse1I ACTGG 1 cut(s) 1133
Bse3DI GCAATG 1 cut(s) 882
Bse8I GATNNNNATC 1 cut(s) 1059
BseAI TCCGGA 1 cut(s) 248
BseBI CCWGG 1 cut(s) 685
BseCI ATCGAT 1 cut(s) 545
BseGI GGATG 4 cut(s) 460, 706, 746, 1063
BseJI GATNNNNATC 1 cut(s) 1059
BseLI CCNNNNNNNGG 2 cut(s) 282, 343
BseMI GCAATG 1 cut(s) 882
BseMII CTCAG 1 cut(s) 1156
BseNI ACTGG 1 cut(s) 1133
BseRI GAGGAG 2 cut(s) 414, 417
BseX3I CGGCCG 1 cut(s) 265
BseXI GCAGC 2 cut(s) 82, 1088
Bsh1236I CGCG 5 cut(s) 332, 356, 672, 989, 1094
Bsh1285I CGRYCG 1 cut(s) 268
BshFI GGCC 2 cut(s) 5, 267
BshNI GGYRCC 1 cut(s) 346
BshVI ATCGAT 1 cut(s) 545
BsiEI CGRYCG 1 cut(s) 268
BsiHKAI GWGCWC 1 cut(s) 918
BsiSI CCGG 7 cut(s) 177, 249, 264, 268, 378, 423, 870
BslI CCNNNNNNNGG 2 cut(s) 282, 343
BsmAI GTCTC 1 cut(s) 37
BsmI GAATGC 1 cut(s) 421
BsnI GGCC 2 cut(s) 5, 267
Bsp1286I GDGCHC 1 cut(s) 918
Bsp13I TCCGGA 1 cut(s) 248
Bsp1407I TGTACA 1 cut(s) 457
Bsp143I GATC 6 cut(s) 317, 333, 499, 717, 901, 1054
Bsp68I TCGCGA 1 cut(s) 1094
BspACI CCGC 5 cut(s) 129, 132, 969, 989, 1005
BspANI GGCC 2 cut(s) 5, 267
BspCNI CTCAG 1 cut(s) 1155
BspDI ATCGAT 1 cut(s) 545
BspEI TCCGGA 1 cut(s) 248
BspFNI CGCG 5 cut(s) 332, 356, 672, 989, 1094
BspHI TCATGA 1 cut(s) 411
BspLI GGNNCC 2 cut(s) 348, 1056
BspPI GGATC 4 cut(s) 325, 328, 1049, 1062
BspT107I GGYRCC 1 cut(s) 346
BsrDI GCAATG 1 cut(s) 882
BsrFI RCCGGY 2 cut(s) 263, 267
BsrGI TGTACA 1 cut(s) 457
BsrI ACTGG 1 cut(s) 1133
BssAI RCCGGY 2 cut(s) 263, 267
BssMI GATC 6 cut(s) 317, 333, 499, 717, 901, 1054
Bst2UI CCWGG 1 cut(s) 685
Bst4CI ACNGT 3 cut(s) 492, 621, 750
Bst6I CTCTTC 1 cut(s) 1023
BstAUI TGTACA 1 cut(s) 457
BstDEI CTNAG 2 cut(s) 1142, 1151
BstF5I GGATG 4 cut(s) 460, 706, 746, 1063
BstFNI CGCG 5 cut(s) 332, 356, 672, 989, 1094
BstKTI GATC 6 cut(s) 320, 336, 502, 720, 904, 1057
BstMAI GTCTC 1 cut(s) 37
BstMBI GATC 6 cut(s) 317, 333, 499, 717, 901, 1054
BstMCI CGRYCG 1 cut(s) 268
BstMWI GCNNNNNNNGC 5 cut(s) 11, 329, 353, 374, 959
BstNI CCWGG 1 cut(s) 685
BstNSI RCATGY 1 cut(s) 658
BstSCI CCNGG 3 cut(s) 422, 683, 868
BstSFI CTRYAG 1 cut(s) 561
BstUI CGCG 5 cut(s) 332, 356, 672, 989, 1094
BstV1I GCAGC 2 cut(s) 82, 1088
BstX2I RGATCY 1 cut(s) 1054
BstYI RGATCY 1 cut(s) 1054
BstZI CGGCCG 1 cut(s) 265
Bsu15I ATCGAT 1 cut(s) 545
BsuRI GGCC 2 cut(s) 5, 267
BsuTUI ATCGAT 1 cut(s) 545
BtgZI GCGATG 1 cut(s) 1075
BtsCI GGATG 4 cut(s) 460, 706, 746, 1063
BtsI GCAGTG 1 cut(s) 78
BtsIMutI CAGTG 1 cut(s) 78
BtuMI TCGCGA 1 cut(s) 1094
CciI TCATGA 1 cut(s) 411
Cfr10I RCCGGY 2 cut(s) 263, 267
Cfr13I GGNCC 3 cut(s) 285, 731, 1007
ClaI ATCGAT 1 cut(s) 545
CpoI CGGWCCG 1 cut(s) 1007
CseI GACGC 1 cut(s) 362
Csp6I GTAC 2 cut(s) 458, 751
CspI CGGWCCG 1 cut(s) 1007
CviAII CATG 4 cut(s) 412, 655, 887, 910
CviQI GTAC 2 cut(s) 458, 751
DdeI CTNAG 2 cut(s) 1142, 1151
DpnI GATC 6 cut(s) 319, 335, 501, 719, 903, 1056
DpnII GATC 6 cut(s) 317, 333, 499, 717, 901, 1054
EaeI YGGCCR 2 cut(s) 3, 265
EagI CGGCCG 1 cut(s) 265
Eam1104I CTCTTC 1 cut(s) 1023
EarI CTCTTC 1 cut(s) 1023
EciI GGCGGA 1 cut(s) 147
Ecl136II GAGCTC 1 cut(s) 916
EclXI CGGCCG 1 cut(s) 265
Eco24I GRGCYC 1 cut(s) 918
Eco32I GATATC 1 cut(s) 298
Eco47I GGWCC 3 cut(s) 285, 731, 1007
Eco52I CGGCCG 1 cut(s) 265
Eco53kI GAGCTC 1 cut(s) 916
Eco57I CTGAAG 1 cut(s) 69
EcoICRI GAGCTC 1 cut(s) 916
EcoRII CCWGG 1 cut(s) 683
EcoRV GATATC 1 cut(s) 298
EcoT22I ATGCAT 2 cut(s) 394, 746
EcoT38I GRGCYC 1 cut(s) 918
FaeI CATG 4 cut(s) 415, 658, 890, 913
FatI CATG 4 cut(s) 411, 654, 886, 909
FauI CCCGC 1 cut(s) 982
FblI GTMKAC 1 cut(s) 897
Fnu4HI GCNGC 3 cut(s) 96, 130, 1077
FokI GGATG 4 cut(s) 467, 713, 753, 1050
FriOI GRGCYC 1 cut(s) 918
Fsp4HI GCNGC 3 cut(s) 96, 130, 1077
FspBI CTAG 4 cut(s) 24, 167, 207, 809
GluI GCNGC 3 cut(s) 96, 130, 1077
GsuI CTGGAG 3 cut(s) 21, 417, 1116
HaeIII GGCC 2 cut(s) 5, 267
HapII CCGG 7 cut(s) 177, 249, 264, 268, 378, 423, 870
HgaI GACGC 1 cut(s) 362
Hin1II CATG 4 cut(s) 415, 658, 890, 913
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HindIII AAGCTT 1 cut(s) 366
HinfI GANTC 4 cut(s) 30, 161, 408, 429
HpaI GTTAAC 1 cut(s) 112
HpaII CCGG 7 cut(s) 177, 249, 264, 268, 378, 423, 870
HphI GGTGA 3 cut(s) 265, 305, 1124
Hpy166II GTNNAC 5 cut(s) 112, 273, 385, 624, 898
Hpy188I TCNGA 3 cut(s) 158, 317, 1068
Hpy188III TCNNGA 6 cut(s) 249, 396, 412, 635, 721, 1093
Hpy8I GTNNAC 5 cut(s) 112, 273, 385, 624, 898
Hpy99I CGWCG 1 cut(s) 202
HpyAV CCTTC 1 cut(s) 352
HpyCH4III ACNGT 3 cut(s) 492, 621, 750
HpyCH4IV ACGT 3 cut(s) 200, 726, 894
HpyCH4V TGCA 3 cut(s) 392, 744, 890
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 329, 353, 374, 959
HpyF3I CTNAG 2 cut(s) 1142, 1151
HpySE526I ACGT 3 cut(s) 200, 726, 894
Hsp92II CATG 4 cut(s) 415, 658, 890, 913
Kpn2I TCCGGA 1 cut(s) 248
KspAI GTTAAC 1 cut(s) 112
Kzo9I GATC 6 cut(s) 317, 333, 499, 717, 901, 1054
LmnI GCTCC 4 cut(s) 140, 566, 872, 913
Lsp1109I GCAGC 2 cut(s) 82, 1088
LweI GCATC 4 cut(s) 163, 401, 691, 731
MaeI CTAG 4 cut(s) 24, 167, 207, 809
MaeII ACGT 3 cut(s) 200, 726, 894
MalI GATC 6 cut(s) 319, 335, 501, 719, 903, 1056
MboI GATC 6 cut(s) 317, 333, 499, 717, 901, 1054
MboII GAAGA 5 cut(s) 52, 55, 320, 463, 1040
MfeI CAATTG 2 cut(s) 695, 1100
MflI RGATCY 1 cut(s) 1054
MhlI GDGCHC 1 cut(s) 918
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 187, 586, 695, 770, 996, 1100
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 39
MmeI TCCRAC 1 cut(s) 709
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 2 cut(s) 394, 746
MroI TCCGGA 1 cut(s) 248
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 111, 833, 1119, 1137
MslI CAYNNNNRTG 1 cut(s) 1068
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 7 cut(s) 177, 249, 264, 268, 378, 423, 870
MspR9I CCNGG 3 cut(s) 424, 685, 870
MunI CAATTG 2 cut(s) 695, 1100
Mva1269I GAATGC 1 cut(s) 421
MvaI CCWGG 1 cut(s) 685
MvnI CGCG 5 cut(s) 332, 356, 672, 989, 1094
MwoI GCNNNNNNNGC 5 cut(s) 11, 329, 353, 374, 959
NciI CCSGG 2 cut(s) 424, 870
NdeII GATC 6 cut(s) 317, 333, 499, 717, 901, 1054
NlaIII CATG 4 cut(s) 415, 658, 890, 913
NlaIV GGNNCC 2 cut(s) 348, 1056
NruI TCGCGA 1 cut(s) 1094
NsiI ATGCAT 2 cut(s) 394, 746
NspI RCATGY 1 cut(s) 658
PagI TCATGA 1 cut(s) 411
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PctI GAATGC 1 cut(s) 421
PfeI GAWTC 3 cut(s) 161, 408, 429
PflFI GACNNNGTC 1 cut(s) 202
PfoI TCCNGGA 1 cut(s) 868
PkrI GCNGC 3 cut(s) 97, 131, 1078
PleI GAGTC 1 cut(s) 38
PpsI GAGTC 1 cut(s) 38
Psp124BI GAGCTC 1 cut(s) 918
Psp1406I AACGTT 1 cut(s) 726
Psp6I CCWGG 1 cut(s) 683
PspGI CCWGG 1 cut(s) 683
PspN4I GGNNCC 2 cut(s) 348, 1056
PspPI GGNCC 3 cut(s) 285, 731, 1007
PsuI RGATCY 1 cut(s) 1054
PsyI GACNNNGTC 1 cut(s) 202
RruI TCGCGA 1 cut(s) 1094
RsaI GTAC 2 cut(s) 459, 752
RsaNI GTAC 2 cut(s) 458, 751
RseI CAYNNNNRTG 1 cut(s) 1068
Rsr2I CGGWCCG 1 cut(s) 1007
RsrII CGGWCCG 1 cut(s) 1007
SacI GAGCTC 1 cut(s) 918
SaqAI TTAA 4 cut(s) 111, 833, 1119, 1137
SatI GCNGC 3 cut(s) 96, 130, 1077
Sau3AI GATC 6 cut(s) 317, 333, 499, 717, 901, 1054
Sau96I GGNCC 3 cut(s) 285, 731, 1007
SchI GAGTC 1 cut(s) 39
ScrFI CCNGG 3 cut(s) 424, 685, 870
SduI GDGCHC 1 cut(s) 918
SfaNI GCATC 4 cut(s) 163, 401, 691, 731
SfcI CTRYAG 1 cut(s) 561
SinI GGWCC 3 cut(s) 285, 731, 1007
SmiMI CAYNNNNRTG 1 cut(s) 1068
Sse9I AATT 6 cut(s) 187, 586, 695, 770, 996, 1100
SsiI CCGC 5 cut(s) 129, 132, 969, 989, 1005
SspMI CTAG 4 cut(s) 24, 167, 207, 809
SstI GAGCTC 1 cut(s) 918
StyD4I CCNGG 3 cut(s) 422, 683, 868
TaaI ACNGT 3 cut(s) 492, 621, 750
TaiI ACGT 3 cut(s) 203, 729, 897
TaqI TCGA 4 cut(s) 171, 197, 545, 1016
TaqII GACCGA 2 cut(s) 748, 1024
TasI AATT 6 cut(s) 187, 586, 695, 770, 996, 1100
TatI WGTACW 1 cut(s) 457
TauI GCSGC 1 cut(s) 132
TfiI GAWTC 3 cut(s) 161, 408, 429
Tru1I TTAA 4 cut(s) 111, 833, 1119, 1137
Tru9I TTAA 4 cut(s) 111, 833, 1119, 1137
TscAI CASTG 1 cut(s) 85
TseI GCWGC 2 cut(s) 95, 1076
TspDTI ATGAA 3 cut(s) 139, 233, 428
TspGWI ACGGA 3 cut(s) 298, 499, 990
TspRI CASTG 1 cut(s) 85
Tth111I GACNNNGTC 1 cut(s) 202
VpaK11BI GGWCC 3 cut(s) 285, 731, 1007
XceI RCATGY 1 cut(s) 658
XmiI GTMKAC 1 cut(s) 897
XspI CTAG 4 cut(s) 24, 167, 207, 809
Zsp2I ATGCAT 2 cut(s) 394, 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.