FvH4_1g14780

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0015376
Physical Location & Seq
Forward (+)
8263335 .. 8265904
2570 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g14780.t1

Sequence Viewer

Length: 1338 bp
ATGTTGATGAACTACCTCATCAATATTCCCAACTCATCAACTAGCTCACGCTTGTACCACTATACAAAAAGGCAGTCATACCCAATATTACGAGACTTGGTTAACATAATCTTACAAGTTTCTCCAATTAATGGATGCTTTTGTGAACAAACATGGTGCTTATATACTTCTCAGTATGCTAAGTGTGAAGCAATCATGATCTTAGCCATGGCCATAACAAGATCTTATATCACTCCGGCCTTGGTAGGCTTGGTTCTTTTACTTATCAACAACCTGAGTGATTTTGCATATGCTGCTGAGAGTGAAGGGTTAGGAGCTTCTTTTATCTTTGGTGATTCCCTGGTTGATTCCGGAAACAATAACTACTTGCCGACACTGTCCAAGGCGAATGTCCGTCCTAATGGGATAGATTTCAAGGCATCCGGAGGAAATCCCACCGGACGGTACACCAATGGTAGAACTATTGGTGACATAGTTGGTGAAGAACTGGGACAACCAAATTACGCAGTCCCATATTTAGCTCCTAACGCTACAGGGAAAGCAATATTGTATGGGGTCAATTATGCTTCAGGAGGAGCAGGGATCATGAATGCAACTGGAAGAATATTTGTAAATAGGGTCGGAATGGATATCCAAATCGATTATTTCAACGAAACAAGGAAGCAGATTGATAAGCTACTGGGTCCATCAAAGGCAAAAGAGTACGTTTCGAAGAGGTCTATTTTCTCAATCACAGTGGGATCAAATGACTTTCTGAACAATTATATGCTACCAGTCATCTCCATTGGAGCAAGAATTTCTCAGACCCCAGATGCATTCGTTGATGATTTGATCACTCACTTCAGGGCTCAACTCACTAGACTTTATCAATTGGGTGCTCGAAAATTTGTGATAGGAAATGTTGGACCTATAGGTTGCATACCGTATCAGAAGACCATCAATCAGCTCAACCAAGACCAATGTGTGGAGTTGCCTAATAAGCTTGCGGTGAAATACAATGGCAGATTGAAAGACTTGCTTGCTGAGCTGAATGACAACCTCCCCGGATCAACATTTGTTTACGCAAATGTGTATGATCTAGTGTCGGAGCTCATTACTAACTACGATAAATATGGATTTACAACTGCAAGTAGAGCTTGCTGTGGGAATGGTGGCCAATATGCAGGAATAGTTCCATGTGGTCCAACATCAAGTATGTGTTCTGATCGGTCCAAGCATGTATTCTGGGATCCCTACCATCCAAGTGAAGCTGCCAACCTTATACTTGCCAAGAAATTGGTTGATGGAGACACAAGAGTCATTTCTCCAATGAATCTCAGGCAACTTCGAGACCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

446

Amino Acids

48.95

Weight (kDa)

8.53

Isoelectric Point (pI)

33.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 108 - 426 3.2e-34 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23540
fragaria_vesca FvH4_1g14780
malus_domestica MD02G1161000.v1.1 MD15G1275100.v1.1
prunus_persica Prupe.7G142600_v2.0.a1
pyrus_communis pycom02g12710 pycom15g23820
rosa_chinensis RchiOBHm_Chr2g0103741
rosa_laevigata RLG00000017253
rosa_multiflora Rmu_sc0001852.1_g000002
rosa_roxburghii Rroxscaffold_2G00139440
rosa_rugosa Rorug02G0117000
rosa_samantha Rh2AG165800 Rh2BG173100 Rh2CG171800
rosa_wichuraiana Rw2G012990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1295
AccB7I CCANNNNNTGG 2 cut(s) 131, 964
AccIII TCCGGA 2 cut(s) 350, 422
AciI CCGC 1 cut(s) 986
AclWI GGATC 5 cut(s) 590, 748, 1054, 1223, 1236
AcoI YGGCCR 2 cut(s) 210, 1153
AcsI RAATTY 2 cut(s) 795, 884
AcuI CTGAAG 2 cut(s) 552, 826
AfaI GTAC 3 cut(s) 56, 446, 704
AfiI CCNNNNNNNGG 3 cut(s) 131, 441, 964
AgsI TTSAA 3 cut(s) 415, 649, 1009
AjnI CCWGG 1 cut(s) 339
AjuI GAANNNNNNNTTGG 2 cut(s) 1205, 1237
Alw21I GWGCWC 2 cut(s) 880, 1092
Alw26I GTCTC 3 cut(s) 87, 1281, 1323
AlwI GGATC 5 cut(s) 590, 748, 1054, 1223, 1236
Aor13HI TCCGGA 2 cut(s) 350, 422
AoxI GGCC 3 cut(s) 210, 237, 1153
ApeKI GCWGC 2 cut(s) 293, 1250
ApoI RAATTY 2 cut(s) 795, 884
ArsI GACNNNNNNTTYG 2 cut(s) 59, 91
AseI ATTAAT 1 cut(s) 129
AspS9I GGNCC 4 cut(s) 683, 905, 1181, 1209
AsuC2I CCSGG 1 cut(s) 1044
AsuHPI GGTGA 4 cut(s) 344, 479, 491, 1000
AsuII TTCGAA 1 cut(s) 710
AvaII GGWCC 4 cut(s) 683, 905, 1181, 1209
BaeI ACNNNNGTAYC 2 cut(s) 38, 71
BalI TGGCCA 2 cut(s) 212, 1155
BamHI GGATCC 1 cut(s) 1228
BanII GRGCYC 2 cut(s) 850, 1092
BbsI GAAGAC 1 cut(s) 938
Bbv12I GWGCWC 2 cut(s) 880, 1092
BbvI GCAGC 2 cut(s) 280, 1237
BccI CCATC 4 cut(s) 694, 944, 1245, 1277
BciT130I CCWGG 1 cut(s) 341
BclI TGATCA 1 cut(s) 831
BcnI CCSGG 1 cut(s) 1044
BcoDI GTCTC 3 cut(s) 87, 1281, 1323
BfaI CTAG 3 cut(s) 42, 858, 1079
BfmI CTRYAG 2 cut(s) 531, 909
BglII AGATCT 1 cut(s) 221
BisI GCNGC 2 cut(s) 294, 1251
BlpI GCTNAGC 1 cut(s) 1023
BlsI GCNGC 2 cut(s) 295, 1252
Bme1390I CCNGG 2 cut(s) 341, 1044
Bme18I GGWCC 4 cut(s) 683, 905, 1181, 1209
BmgT120I GGNCC 4 cut(s) 683, 905, 1181, 1209
BmiI GGNNCC 2 cut(s) 684, 1230
BmrFI CCNGG 2 cut(s) 341, 1044
BmrI ACTGGG 2 cut(s) 497, 689
BmsI GCATC 3 cut(s) 125, 428, 802
BmuI ACTGGG 2 cut(s) 497, 689
BpiI GAAGAC 1 cut(s) 938
Bpu1102I GCTNAGC 1 cut(s) 1023
Bpu14I TTCGAA 1 cut(s) 710
BpuMI CCSGG 1 cut(s) 1044
Bsa29I ATCGAT 1 cut(s) 639
BsaI GGTCTC 1 cut(s) 1323
BsaJI CCNNGG 5 cut(s) 207, 240, 339, 381, 1042
BsaWI WCCGGW 3 cut(s) 350, 422, 437
Bsc4I CCNNNNNNNGG 3 cut(s) 131, 441, 964
Bse1I ACTGG 4 cut(s) 492, 601, 684, 773
BseAI TCCGGA 2 cut(s) 350, 422
BseBI CCWGG 1 cut(s) 341
BseCI ATCGAT 1 cut(s) 639
BseDI CCNNGG 5 cut(s) 207, 240, 339, 381, 1042
BseGI GGATG 3 cut(s) 140, 419, 1237
BseLI CCNNNNNNNGG 3 cut(s) 131, 441, 964
BseMII CTCAG 6 cut(s) 185, 266, 288, 815, 1014, 1330
BseNI ACTGG 4 cut(s) 492, 601, 684, 773
BseRI GAGGAG 1 cut(s) 588
BseXI GCAGC 2 cut(s) 280, 1237
BshFI GGCC 3 cut(s) 212, 239, 1155
BshVI ATCGAT 1 cut(s) 639
BsiHKAI GWGCWC 2 cut(s) 880, 1092
BsiSI CCGG 5 cut(s) 236, 351, 423, 438, 1044
BslFI GGGAC 2 cut(s) 494, 504
BslI CCNNNNNNNGG 3 cut(s) 131, 441, 964
BsmAI GTCTC 3 cut(s) 87, 1281, 1323
BsmFI GGGAC 2 cut(s) 494, 504
BsmI GAATGC 2 cut(s) 595, 815
BsnI GGCC 3 cut(s) 212, 239, 1155
Bso31I GGTCTC 1 cut(s) 1323
Bsp119I TTCGAA 1 cut(s) 710
Bsp1286I GDGCHC 3 cut(s) 850, 880, 1092
Bsp13I TCCGGA 2 cut(s) 350, 422
Bsp143I GATC 9 cut(s) 198, 221, 582, 740, 831, 1046, 1075, 1204, 1228
Bsp1720I GCTNAGC 1 cut(s) 1023
Bsp19I CCATGG 1 cut(s) 207
BspACI CCGC 1 cut(s) 986
BspANI GGCC 3 cut(s) 212, 239, 1155
BspCNI CTCAG 6 cut(s) 184, 267, 289, 814, 1015, 1329
BspDI ATCGAT 1 cut(s) 639
BspEI TCCGGA 2 cut(s) 350, 422
BspHI TCATGA 2 cut(s) 195, 585
BspLI GGNNCC 2 cut(s) 684, 1230
BspPI GGATC 5 cut(s) 590, 748, 1054, 1223, 1236
BspT104I TTCGAA 1 cut(s) 710
BspTNI GGTCTC 1 cut(s) 1323
BsrI ACTGG 4 cut(s) 492, 601, 684, 773
BssECI CCNNGG 5 cut(s) 207, 240, 339, 381, 1042
BssMI GATC 9 cut(s) 198, 221, 582, 740, 831, 1046, 1075, 1204, 1228
BssT1I CCWWGG 3 cut(s) 207, 240, 381
Bst2UI CCWGG 1 cut(s) 341
Bst4CI ACNGT 4 cut(s) 378, 444, 736, 924
Bst6I CTCTTC 1 cut(s) 707
BstAPI GCANNNNNTGC 1 cut(s) 293
BstBI TTCGAA 1 cut(s) 710
BstC8I GCNNGC 3 cut(s) 984, 1020, 1138
BstDEI CTNAG 8 cut(s) 171, 180, 202, 275, 297, 801, 1023, 1316
BstDSI CCRYGG 1 cut(s) 207
BstF5I GGATG 3 cut(s) 140, 419, 1237
BstKTI GATC 9 cut(s) 201, 224, 585, 743, 834, 1049, 1078, 1207, 1231
BstMAI GTCTC 3 cut(s) 87, 1281, 1323
BstMBI GATC 9 cut(s) 198, 221, 582, 740, 831, 1046, 1075, 1204, 1228
BstMWI GCNNNNNNNGC 5 cut(s) 293, 527, 979, 1024, 1133
BstNI CCWGG 1 cut(s) 341
BstNSI RCATGY 1 cut(s) 1220
BstSCI CCNGG 2 cut(s) 339, 1042
BstSFI CTRYAG 2 cut(s) 531, 909
BstV1I GCAGC 2 cut(s) 280, 1237
BstV2I GAAGAC 1 cut(s) 938
BstX2I RGATCY 2 cut(s) 221, 1228
BstXI CCANNNNNNTGG 1 cut(s) 1276
BstYI RGATCY 2 cut(s) 221, 1228
Bsu15I ATCGAT 1 cut(s) 639
BsuRI GGCC 3 cut(s) 212, 239, 1155
BsuTUI ATCGAT 1 cut(s) 639
BtgI CCRYGG 1 cut(s) 207
BtsCI GGATG 3 cut(s) 140, 419, 1237
BtsIMutI CAGTG 2 cut(s) 374, 741
Cac8I GCNNGC 3 cut(s) 984, 1020, 1138
CciI TCATGA 2 cut(s) 195, 585
Cfr13I GGNCC 4 cut(s) 683, 905, 1181, 1209
ClaI ATCGAT 1 cut(s) 639
Csp6I GTAC 3 cut(s) 55, 445, 703
CspCI CAANNNNNGTGG 2 cut(s) 717, 752
CviAII CATG 6 cut(s) 153, 196, 208, 586, 1176, 1217
CviQI GTAC 3 cut(s) 55, 445, 703
DdeI CTNAG 8 cut(s) 171, 180, 202, 275, 297, 801, 1023, 1316
DpnI GATC 9 cut(s) 200, 223, 584, 742, 833, 1048, 1077, 1206, 1230
DpnII GATC 9 cut(s) 198, 221, 582, 740, 831, 1046, 1075, 1204, 1228
DrdI GACNNNNNNGTC 1 cut(s) 1295
DseDI GACNNNNNNGTC 1 cut(s) 1295
EaeI YGGCCR 2 cut(s) 210, 1153
Eam1104I CTCTTC 1 cut(s) 707
EarI CTCTTC 1 cut(s) 707
Ecl136II GAGCTC 1 cut(s) 1090
Eco130I CCWWGG 3 cut(s) 207, 240, 381
Eco24I GRGCYC 2 cut(s) 850, 1092
Eco31I GGTCTC 1 cut(s) 1323
Eco32I GATATC 1 cut(s) 631
Eco47I GGWCC 4 cut(s) 683, 905, 1181, 1209
Eco53kI GAGCTC 1 cut(s) 1090
Eco57I CTGAAG 2 cut(s) 552, 826
EcoICRI GAGCTC 1 cut(s) 1090
EcoRII CCWGG 1 cut(s) 339
EcoRV GATATC 1 cut(s) 631
EcoT14I CCWWGG 3 cut(s) 207, 240, 381
EcoT22I ATGCAT 1 cut(s) 817
EcoT38I GRGCYC 2 cut(s) 850, 1092
ErhI CCWWGG 3 cut(s) 207, 240, 381
FaeI CATG 6 cut(s) 156, 199, 211, 589, 1179, 1220
FalI AAGNNNNNCTT 4 cut(s) 1002, 1034, 1120, 1152
FaqI GGGAC 2 cut(s) 494, 504
FatI CATG 6 cut(s) 152, 195, 207, 585, 1175, 1216
FauNDI CATATG 1 cut(s) 289
FbaI TGATCA 1 cut(s) 831
Fnu4HI GCNGC 2 cut(s) 294, 1251
FokI GGATG 3 cut(s) 147, 406, 1224
FriOI GRGCYC 2 cut(s) 850, 1092
Fsp4HI GCNGC 2 cut(s) 294, 1251
FspBI CTAG 3 cut(s) 42, 858, 1079
GluI GCNGC 2 cut(s) 294, 1251
HaeIII GGCC 3 cut(s) 212, 239, 1155
HapII CCGG 5 cut(s) 236, 351, 423, 438, 1044
Hin1II CATG 6 cut(s) 156, 199, 211, 589, 1179, 1220
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HindIII AAGCTT 1 cut(s) 980
HinfI GANTC 4 cut(s) 335, 347, 1296, 1312
HpaI GTTAAC 1 cut(s) 103
HpaII CCGG 5 cut(s) 236, 351, 423, 438, 1044
HphI GGTGA 4 cut(s) 344, 479, 491, 1000
Hpy166II GTNNAC 4 cut(s) 103, 146, 447, 1060
Hpy188I TCNGA 6 cut(s) 623, 756, 804, 930, 1087, 1204
Hpy188III TCNNGA 6 cut(s) 196, 351, 423, 570, 586, 1328
Hpy8I GTNNAC 4 cut(s) 103, 146, 447, 1060
HpyAV CCTTC 1 cut(s) 299
HpyCH4III ACNGT 4 cut(s) 378, 444, 736, 924
HpyCH4IV ACGT 1 cut(s) 705
HpyCH4V TGCA 6 cut(s) 287, 593, 815, 918, 1127, 1163
HpyF10VI GCNNNNNNNGC 5 cut(s) 293, 527, 979, 1024, 1133
HpyF3I CTNAG 8 cut(s) 171, 180, 202, 275, 297, 801, 1023, 1316
HpySE526I ACGT 1 cut(s) 705
Hsp92II CATG 6 cut(s) 156, 199, 211, 589, 1179, 1220
Kpn2I TCCGGA 2 cut(s) 350, 422
Ksp22I TGATCA 1 cut(s) 831
KspAI GTTAAC 1 cut(s) 103
Kzo9I GATC 9 cut(s) 198, 221, 582, 740, 831, 1046, 1075, 1204, 1228
LmnI GCTCC 5 cut(s) 314, 526, 575, 788, 1087
Lsp1109I GCAGC 2 cut(s) 280, 1237
LweI GCATC 3 cut(s) 125, 428, 802
MaeI CTAG 3 cut(s) 42, 858, 1079
MaeII ACGT 1 cut(s) 705
MaeIII GTNAC 1 cut(s) 467
MalI GATC 9 cut(s) 200, 223, 584, 742, 833, 1048, 1077, 1206, 1230
MboI GATC 9 cut(s) 198, 221, 582, 740, 831, 1046, 1075, 1204, 1228
MboII GAAGA 4 cut(s) 494, 612, 724, 943
MfeI CAATTG 1 cut(s) 869
MflI RGATCY 2 cut(s) 221, 1228
MhlI GDGCHC 3 cut(s) 850, 880, 1092
MlsI TGGCCA 2 cut(s) 212, 1155
MluCI AATT 8 cut(s) 126, 499, 559, 760, 795, 869, 884, 1274
MluNI TGGCCA 2 cut(s) 212, 1155
MlyI GAGTC 1 cut(s) 1305
MmeI TCCRAC 4 cut(s) 601, 883, 1065, 1208
MnlI CCTC 5 cut(s) 26, 419, 566, 708, 1049
Mox20I TGGCCA 2 cut(s) 212, 1155
Mph1103I ATGCAT 1 cut(s) 817
MroI TCCGGA 2 cut(s) 350, 422
MscI TGGCCA 2 cut(s) 212, 1155
MseI TTAA 3 cut(s) 102, 129, 1336
MslI CAYNNNNRTG 1 cut(s) 1242
Msp20I TGGCCA 2 cut(s) 212, 1155
MspI CCGG 5 cut(s) 236, 351, 423, 438, 1044
MspR9I CCNGG 2 cut(s) 341, 1044
MunI CAATTG 1 cut(s) 869
Mva1269I GAATGC 2 cut(s) 595, 815
MvaI CCWGG 1 cut(s) 341
MwoI GCNNNNNNNGC 5 cut(s) 293, 527, 979, 1024, 1133
NciI CCSGG 1 cut(s) 1044
NcoI CCATGG 1 cut(s) 207
NdeI CATATG 1 cut(s) 289
NdeII GATC 9 cut(s) 198, 221, 582, 740, 831, 1046, 1075, 1204, 1228
NlaIII CATG 6 cut(s) 156, 199, 211, 589, 1179, 1220
NlaIV GGNNCC 2 cut(s) 684, 1230
NmuCI GTSAC 1 cut(s) 467
NsiI ATGCAT 1 cut(s) 817
NspI RCATGY 1 cut(s) 1220
NspV TTCGAA 1 cut(s) 710
PagI TCATGA 2 cut(s) 195, 585
PctI GAATGC 2 cut(s) 595, 815
PfeI GAWTC 3 cut(s) 335, 347, 1312
PflFI GACNNNGTC 1 cut(s) 376
PflMI CCANNNNNTGG 2 cut(s) 131, 964
PkrI GCNGC 2 cut(s) 295, 1252
PleI GAGTC 1 cut(s) 1304
PpsI GAGTC 1 cut(s) 1304
PshBI ATTAAT 1 cut(s) 129
Psp124BI GAGCTC 1 cut(s) 1092
Psp6I CCWGG 1 cut(s) 339
PspGI CCWGG 1 cut(s) 339
PspN4I GGNNCC 2 cut(s) 684, 1230
PspPI GGNCC 4 cut(s) 683, 905, 1181, 1209
PsrI GAACNNNNNNTAC 2 cut(s) 237, 269
PsuI RGATCY 2 cut(s) 221, 1228
PsyI GACNNNGTC 1 cut(s) 376
RsaI GTAC 3 cut(s) 56, 446, 704
RsaNI GTAC 3 cut(s) 55, 445, 703
RseI CAYNNNNRTG 1 cut(s) 1242
SacI GAGCTC 1 cut(s) 1092
SaqAI TTAA 3 cut(s) 102, 129, 1336
SatI GCNGC 2 cut(s) 294, 1251
Sau3AI GATC 9 cut(s) 198, 221, 582, 740, 831, 1046, 1075, 1204, 1228
Sau96I GGNCC 4 cut(s) 683, 905, 1181, 1209
SchI GAGTC 1 cut(s) 1305
ScrFI CCNGG 2 cut(s) 341, 1044
SduI GDGCHC 3 cut(s) 850, 880, 1092
SfaNI GCATC 3 cut(s) 125, 428, 802
SfcI CTRYAG 2 cut(s) 531, 909
SfuI TTCGAA 1 cut(s) 710
SinI GGWCC 4 cut(s) 683, 905, 1181, 1209
SmiMI CAYNNNNRTG 1 cut(s) 1242
Sse9I AATT 8 cut(s) 126, 499, 559, 760, 795, 869, 884, 1274
SsiI CCGC 1 cut(s) 986
SspI AATATT 4 cut(s) 25, 87, 546, 606
SspMI CTAG 3 cut(s) 42, 858, 1079
SstI GAGCTC 1 cut(s) 1092
StyD4I CCNGG 2 cut(s) 339, 1042
StyI CCWWGG 3 cut(s) 207, 240, 381
TaaI ACNGT 4 cut(s) 378, 444, 736, 924
TaiI ACGT 1 cut(s) 708
TaqI TCGA 4 cut(s) 639, 710, 880, 1327
TaqII GACCGA 1 cut(s) 1197
TasI AATT 8 cut(s) 126, 499, 559, 760, 795, 869, 884, 1274
TfiI GAWTC 3 cut(s) 335, 347, 1312
Tru1I TTAA 3 cut(s) 102, 129, 1336
Tru9I TTAA 3 cut(s) 102, 129, 1336
TscAI CASTG 2 cut(s) 381, 741
TseFI GTSAC 1 cut(s) 467
TseI GCWGC 2 cut(s) 293, 1250
Tsp45I GTSAC 1 cut(s) 467
TspDTI ATGAA 3 cut(s) 23, 602, 1325
TspGWI ACGGA 1 cut(s) 383
TspRI CASTG 2 cut(s) 381, 741
Tth111I GACNNNGTC 1 cut(s) 376
Van91I CCANNNNNTGG 2 cut(s) 131, 964
VpaK11BI GGWCC 4 cut(s) 683, 905, 1181, 1209
VspI ATTAAT 1 cut(s) 129
XapI RAATTY 2 cut(s) 795, 884
XceI RCATGY 1 cut(s) 1220
XspI CTAG 3 cut(s) 42, 858, 1079
Zsp2I ATGCAT 1 cut(s) 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.