Rw2G012990

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0015376
Physical Location & Seq
Forward (+)
13556531 .. 13558692
2162 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G012990.1

Sequence Viewer

Length: 933 bp
ATGGATTTCCAGCCATCTGGAGGAAATCCCACCGGGCGGTTCACCAATGGTAGAACCATTGGTGATATAATTGGTGAAGAATTGGGACAACCAAACTATGCAGTTCCATTTTTAGCTCCTAACGCTACAGGGAAAGCAATATTGTATGGGGTGAATTATGCTTCAGGAGGAGCAGGGATCATGAATGCAACAGGAAGAATATTTGTAAATAGGGTGGGGATGGATATCCAAATCGATTATTTCAACGAGACAAGGAAGCAGATTGAAAAGCTATTGGGTCCATCAAAGGCAAAAGAGTACATTTCGAAAAGGTCTATTTTCTCGATCACAGTTGGAGCAAATGACTTTCTGAACAATTACCTACTACCAGTCCTATCCATTGGAGCAAGAATTTCTGAGACCCCAGATGCATTCATTGATGATATGATCACTCACTTCAGGGCTCAACTTACTAGACTTTATCAATTGGGTGCTCGGAAATTTGTGATAGGAAATGTTGGACCAATAGGTTGCATACCTTACCAGAAGACTATCAATCAGCTTGACCAAGACCAATGTGTGGAGTTGCCTAATAAGCTTGCGCTGCAGTACAATGGCAGATTGAAGGACTTGGTTGCTGAGCTGAATGATAACCTCCCTGGATCAACATTTGTTTATGCAAACGTGTATGATCTAGTGTCGGACCTCATTACCAACTACGAAAAATATGGATTTTCAACAGCAAGTAGAGCTTGTTGTGGAAATGGTGGCCAATATGCAGGAATAGTTCCATGTGGTCCAACATCAAGTCTGTGTACTGATCGGTCCAAGCATGTATTCTGGGATCCCTACCATCCAAGTGAAGCTGCCAACATTATACTCGCCAAAAAATTGCTTGATGGAAACACAAGAGTTATTTCTCCAATGAATCTCAGGCAACTTCGAGACCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

310

Amino Acids

34.04

Weight (kDa)

8.13

Isoelectric Point (pI)

30.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 9 - 291 1.4e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23540
fragaria_vesca FvH4_1g14780
malus_domestica MD02G1161000.v1.1 MD15G1275100.v1.1
prunus_persica Prupe.7G142600_v2.0.a1
pyrus_communis pycom02g12710 pycom15g23820
rosa_chinensis RchiOBHm_Chr2g0103741
rosa_laevigata RLG00000017253
rosa_multiflora Rmu_sc0001852.1_g000002
rosa_roxburghii Rroxscaffold_2G00139440
rosa_rugosa Rorug02G0117000
rosa_samantha Rh2AG165800 Rh2BG173100 Rh2CG171800
rosa_wichuraiana Rw2G012990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 559
AciI CCGC 1 cut(s) 37
AclWI GGATC 4 cut(s) 185, 649, 818, 831
AcoI YGGCCR 1 cut(s) 748
AcsI RAATTY 2 cut(s) 390, 479
AcuI CTGAAG 2 cut(s) 147, 421
AfaI GTAC 3 cut(s) 299, 590, 796
AfiI CCNNNNNNNGG 3 cut(s) 20, 36, 559
AflIII ACRYGT 1 cut(s) 663
AgsI TTSAA 4 cut(s) 244, 266, 604, 717
AjnI CCWGG 1 cut(s) 637
AjuI GAANNNNNNNTTGG 2 cut(s) 800, 832
AluBI AGCT 7 cut(s) 116, 271, 541, 577, 622, 731, 845
AluI AGCT 7 cut(s) 116, 271, 541, 577, 622, 731, 845
Alw21I GWGCWC 1 cut(s) 475
Alw26I GTCTC 3 cut(s) 242, 392, 918
AlwI GGATC 4 cut(s) 185, 649, 818, 831
AoxI GGCC 1 cut(s) 748
ApeKI GCWGC 2 cut(s) 583, 845
ApoI RAATTY 2 cut(s) 390, 479
AspLEI GCGC 1 cut(s) 583
AspS9I GGNCC 5 cut(s) 278, 500, 682, 776, 804
AsuC2I CCSGG 1 cut(s) 34
AsuHPI GGTGA 4 cut(s) 34, 74, 86, 163
AsuII TTCGAA 1 cut(s) 305
AvaII GGWCC 5 cut(s) 278, 500, 682, 776, 804
BalI TGGCCA 1 cut(s) 750
BamHI GGATCC 1 cut(s) 823
BanII GRGCYC 1 cut(s) 445
BbsI GAAGAC 1 cut(s) 533
Bbv12I GWGCWC 1 cut(s) 475
BbvI GCAGC 2 cut(s) 570, 832
BccI CCATC 5 cut(s) 22, 214, 289, 840, 872
BciT130I CCWGG 1 cut(s) 639
BclI TGATCA 1 cut(s) 426
BcnI CCSGG 1 cut(s) 34
BcoDI GTCTC 3 cut(s) 242, 392, 918
BfaI CTAG 2 cut(s) 453, 674
BfmI CTRYAG 2 cut(s) 126, 584
BisI GCNGC 2 cut(s) 584, 846
BlpI GCTNAGC 1 cut(s) 618
BlsI GCNGC 2 cut(s) 585, 847
Bme1390I CCNGG 2 cut(s) 34, 639
Bme18I GGWCC 5 cut(s) 278, 500, 682, 776, 804
BmgT120I GGNCC 5 cut(s) 278, 500, 682, 776, 804
BmiI GGNNCC 2 cut(s) 279, 825
BmrFI CCNGG 2 cut(s) 34, 639
BmsI GCATC 1 cut(s) 397
BpiI GAAGAC 1 cut(s) 533
BpmI CTGGAG 1 cut(s) 39
Bpu1102I GCTNAGC 1 cut(s) 618
Bpu14I TTCGAA 1 cut(s) 305
BpuMI CCSGG 1 cut(s) 34
Bsa29I ATCGAT 1 cut(s) 234
BsaBI GATNNNNATC 1 cut(s) 224
BsaI GGTCTC 2 cut(s) 392, 918
BsaJI CCNNGG 1 cut(s) 637
Bsc4I CCNNNNNNNGG 3 cut(s) 20, 36, 559
Bse1I ACTGG 1 cut(s) 368
Bse8I GATNNNNATC 1 cut(s) 224
BseBI CCWGG 1 cut(s) 639
BseCI ATCGAT 1 cut(s) 234
BseDI CCNNGG 1 cut(s) 637
BseGI GGATG 2 cut(s) 225, 832
BseJI GATNNNNATC 1 cut(s) 224
BseLI CCNNNNNNNGG 3 cut(s) 20, 36, 559
BseMII CTCAG 3 cut(s) 387, 609, 925
BseNI ACTGG 1 cut(s) 368
BseRI GAGGAG 1 cut(s) 183
BseXI GCAGC 2 cut(s) 570, 832
BshFI GGCC 1 cut(s) 750
BshVI ATCGAT 1 cut(s) 234
BsiHKAI GWGCWC 1 cut(s) 475
BsiSI CCGG 1 cut(s) 33
BslFI GGGAC 1 cut(s) 99
BslI CCNNNNNNNGG 3 cut(s) 20, 36, 559
BsmAI GTCTC 3 cut(s) 242, 392, 918
BsmFI GGGAC 1 cut(s) 99
BsmI GAATGC 2 cut(s) 190, 410
BsnI GGCC 1 cut(s) 750
Bso31I GGTCTC 2 cut(s) 392, 918
Bsp119I TTCGAA 1 cut(s) 305
Bsp1286I GDGCHC 2 cut(s) 445, 475
Bsp143I GATC 7 cut(s) 177, 324, 426, 641, 670, 799, 823
Bsp1720I GCTNAGC 1 cut(s) 618
BspACI CCGC 1 cut(s) 37
BspANI GGCC 1 cut(s) 750
BspCNI CTCAG 3 cut(s) 388, 610, 924
BspDI ATCGAT 1 cut(s) 234
BspHI TCATGA 1 cut(s) 180
BspLI GGNNCC 2 cut(s) 279, 825
BspMAI CTGCAG 1 cut(s) 588
BspPI GGATC 4 cut(s) 185, 649, 818, 831
BspT104I TTCGAA 1 cut(s) 305
BspTNI GGTCTC 2 cut(s) 392, 918
BsrI ACTGG 1 cut(s) 368
BssECI CCNNGG 1 cut(s) 637
BssMI GATC 7 cut(s) 177, 324, 426, 641, 670, 799, 823
Bst2UI CCWGG 1 cut(s) 639
Bst4CI ACNGT 1 cut(s) 331
BstBI TTCGAA 1 cut(s) 305
BstC8I GCNNGC 1 cut(s) 579
BstDEI CTNAG 3 cut(s) 396, 618, 911
BstF5I GGATG 2 cut(s) 225, 832
BstHHI GCGC 1 cut(s) 583
BstKTI GATC 7 cut(s) 180, 327, 429, 644, 673, 802, 826
BstMAI GTCTC 3 cut(s) 242, 392, 918
BstMBI GATC 7 cut(s) 177, 324, 426, 641, 670, 799, 823
BstMWI GCNNNNNNNGC 4 cut(s) 122, 574, 583, 728
BstNI CCWGG 1 cut(s) 639
BstNSI RCATGY 1 cut(s) 815
BstSCI CCNGG 2 cut(s) 32, 637
BstSFI CTRYAG 2 cut(s) 126, 584
BstV1I GCAGC 2 cut(s) 570, 832
BstV2I GAAGAC 1 cut(s) 533
BstX2I RGATCY 1 cut(s) 823
BstXI CCANNNNNNTGG 1 cut(s) 17
BstYI RGATCY 1 cut(s) 823
Bsu15I ATCGAT 1 cut(s) 234
BsuRI GGCC 1 cut(s) 750
BsuTUI ATCGAT 1 cut(s) 234
BtsCI GGATG 2 cut(s) 225, 832
Cac8I GCNNGC 1 cut(s) 579
CciI TCATGA 1 cut(s) 180
CfoI GCGC 1 cut(s) 583
Cfr13I GGNCC 5 cut(s) 278, 500, 682, 776, 804
ClaI ATCGAT 1 cut(s) 234
Csp6I GTAC 3 cut(s) 298, 589, 795
CviAII CATG 3 cut(s) 181, 771, 812
CviQI GTAC 3 cut(s) 298, 589, 795
DdeI CTNAG 3 cut(s) 396, 618, 911
DpnI GATC 7 cut(s) 179, 326, 428, 643, 672, 801, 825
DpnII GATC 7 cut(s) 177, 324, 426, 641, 670, 799, 823
EaeI YGGCCR 1 cut(s) 748
Eco24I GRGCYC 1 cut(s) 445
Eco31I GGTCTC 2 cut(s) 392, 918
Eco32I GATATC 1 cut(s) 226
Eco47I GGWCC 5 cut(s) 278, 500, 682, 776, 804
Eco57I CTGAAG 2 cut(s) 147, 421
EcoRII CCWGG 1 cut(s) 637
EcoRV GATATC 1 cut(s) 226
EcoT22I ATGCAT 1 cut(s) 412
EcoT38I GRGCYC 1 cut(s) 445
FaeI CATG 3 cut(s) 184, 774, 815
FalI AAGNNNNNCTT 2 cut(s) 715, 747
FaqI GGGAC 1 cut(s) 99
FatI CATG 3 cut(s) 180, 770, 811
FbaI TGATCA 1 cut(s) 426
Fnu4HI GCNGC 2 cut(s) 584, 846
FokI GGATG 2 cut(s) 232, 819
FriOI GRGCYC 1 cut(s) 445
Fsp4HI GCNGC 2 cut(s) 584, 846
FspBI CTAG 2 cut(s) 453, 674
GlaI GCGC 1 cut(s) 582
GluI GCNGC 2 cut(s) 584, 846
GsuI CTGGAG 1 cut(s) 39
HaeIII GGCC 1 cut(s) 750
HapII CCGG 1 cut(s) 33
HhaI GCGC 1 cut(s) 583
Hin1II CATG 3 cut(s) 184, 774, 815
Hin6I GCGC 1 cut(s) 581
HinP1I GCGC 1 cut(s) 581
HindIII AAGCTT 1 cut(s) 575
HinfI GANTC 1 cut(s) 907
HpaII CCGG 1 cut(s) 33
HphI GGTGA 4 cut(s) 34, 74, 86, 163
Hpy166II GTNNAC 2 cut(s) 42, 795
Hpy188I TCNGA 4 cut(s) 351, 397, 477, 682
Hpy188III TCNNGA 5 cut(s) 18, 165, 181, 322, 923
Hpy8I GTNNAC 2 cut(s) 42, 795
HpyAV CCTTC 1 cut(s) 598
HpyCH4III ACNGT 1 cut(s) 331
HpyCH4IV ACGT 1 cut(s) 663
HpyCH4V TGCA 7 cut(s) 101, 188, 410, 513, 586, 659, 758
HpyF10VI GCNNNNNNNGC 4 cut(s) 122, 574, 583, 728
HpyF3I CTNAG 3 cut(s) 396, 618, 911
HpySE526I ACGT 1 cut(s) 663
Hsp92II CATG 3 cut(s) 184, 774, 815
HspAI GCGC 1 cut(s) 581
Ksp22I TGATCA 1 cut(s) 426
Kzo9I GATC 7 cut(s) 177, 324, 426, 641, 670, 799, 823
LmnI GCTCC 4 cut(s) 121, 170, 335, 383
Lsp1109I GCAGC 2 cut(s) 570, 832
LweI GCATC 1 cut(s) 397
MaeI CTAG 2 cut(s) 453, 674
MaeII ACGT 1 cut(s) 663
MalI GATC 7 cut(s) 179, 326, 428, 643, 672, 801, 825
MboI GATC 7 cut(s) 177, 324, 426, 641, 670, 799, 823
MboII GAAGA 3 cut(s) 89, 207, 538
MfeI CAATTG 1 cut(s) 464
MflI RGATCY 1 cut(s) 823
MhlI GDGCHC 2 cut(s) 445, 475
MlsI TGGCCA 1 cut(s) 750
MluCI AATT 8 cut(s) 69, 80, 154, 355, 390, 464, 479, 869
MluNI TGGCCA 1 cut(s) 750
MmeI TCCRAC 4 cut(s) 313, 478, 660, 803
MnlI CCTC 4 cut(s) 14, 161, 644, 695
Mox20I TGGCCA 1 cut(s) 750
Mph1103I ATGCAT 1 cut(s) 412
MscI TGGCCA 1 cut(s) 750
MseI TTAA 1 cut(s) 931
MslI CAYNNNNRTG 1 cut(s) 837
Msp20I TGGCCA 1 cut(s) 750
MspI CCGG 1 cut(s) 33
MspR9I CCNGG 2 cut(s) 34, 639
MunI CAATTG 1 cut(s) 464
Mva1269I GAATGC 2 cut(s) 190, 410
MvaI CCWGG 1 cut(s) 639
MwoI GCNNNNNNNGC 4 cut(s) 122, 574, 583, 728
NciI CCSGG 1 cut(s) 34
NdeII GATC 7 cut(s) 177, 324, 426, 641, 670, 799, 823
NlaIII CATG 3 cut(s) 184, 774, 815
NlaIV GGNNCC 2 cut(s) 279, 825
NsiI ATGCAT 1 cut(s) 412
NspI RCATGY 1 cut(s) 815
NspV TTCGAA 1 cut(s) 305
PagI TCATGA 1 cut(s) 180
PctI GAATGC 2 cut(s) 190, 410
PfeI GAWTC 1 cut(s) 907
PflMI CCANNNNNTGG 1 cut(s) 559
PkrI GCNGC 2 cut(s) 585, 847
Psp6I CCWGG 1 cut(s) 637
PspGI CCWGG 1 cut(s) 637
PspN4I GGNNCC 2 cut(s) 279, 825
PspPI GGNCC 5 cut(s) 278, 500, 682, 776, 804
PstI CTGCAG 1 cut(s) 588
PsuI RGATCY 1 cut(s) 823
RsaI GTAC 3 cut(s) 299, 590, 796
RsaNI GTAC 3 cut(s) 298, 589, 795
RseI CAYNNNNRTG 1 cut(s) 837
SaqAI TTAA 1 cut(s) 931
SatI GCNGC 2 cut(s) 584, 846
Sau3AI GATC 7 cut(s) 177, 324, 426, 641, 670, 799, 823
Sau96I GGNCC 5 cut(s) 278, 500, 682, 776, 804
ScrFI CCNGG 2 cut(s) 34, 639
SduI GDGCHC 2 cut(s) 445, 475
SfaNI GCATC 1 cut(s) 397
SfcI CTRYAG 2 cut(s) 126, 584
SfuI TTCGAA 1 cut(s) 305
SinI GGWCC 5 cut(s) 278, 500, 682, 776, 804
SmiMI CAYNNNNRTG 1 cut(s) 837
Sse9I AATT 8 cut(s) 69, 80, 154, 355, 390, 464, 479, 869
SsiI CCGC 1 cut(s) 37
SspI AATATT 2 cut(s) 141, 201
SspMI CTAG 2 cut(s) 453, 674
StyD4I CCNGG 2 cut(s) 32, 637
TaaI ACNGT 1 cut(s) 331
TaiI ACGT 1 cut(s) 666
TaqI TCGA 4 cut(s) 234, 305, 323, 922
TaqII GACCGA 1 cut(s) 792
TasI AATT 8 cut(s) 69, 80, 154, 355, 390, 464, 479, 869
TatI WGTACW 3 cut(s) 297, 588, 794
TfiI GAWTC 1 cut(s) 907
Tru1I TTAA 1 cut(s) 931
Tru9I TTAA 1 cut(s) 931
TseI GCWGC 2 cut(s) 583, 845
TspDTI ATGAA 3 cut(s) 197, 403, 920
Van91I CCANNNNNTGG 1 cut(s) 559
VpaK11BI GGWCC 5 cut(s) 278, 500, 682, 776, 804
XapI RAATTY 2 cut(s) 390, 479
XceI RCATGY 1 cut(s) 815
XspI CTAG 2 cut(s) 453, 674
Zsp2I ATGCAT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.